Bait Protein: | ASF1 |
Organism: | Saccharomyces cerevisiae |
Publication: | Green EM, Antczak AJ, Bailey AO, Franco AA, Wu KJ, Yates JR 3rd, Kaufman PD. Replication-independent histone deposition by the HIR complex and Asf1. Curr Biol. 2005 Nov 22;15(22):2044-9. |
Comments: | 3912: TAP-tagged, strain background includes hpc2(delta) |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
ASF1 | Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein t... | 78 | 1394 | 70.3% | 0.716 | 31603 |
[ |
RAD53 | Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho... | 90 | 362 | 63.9% | 0.0632 | 91962 |
[ |
gi|7463016|pir||... | lysyl endopeptidase (EC 3.4.21.50) - Lysobacter enzymogenes | 26 | 96 | 61.7% | 0.0512 | 27961 |
[ |
RPS1B | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... | 12 | 28 | 39.6% | 0.0157 | 28812 |
[ |
RPS1A | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... | 11 | 24 | 36.5% | 0.0135 | 28743 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 15 | 24 | 34.3% | 0.00538 | 69470 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 16 | 20 | 34.1% | 0.00468 | 66595 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 16 | 20 | 32.8% | 0.00468 | 66602 |
[ |
contaminant_NRL_... | owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... | 7 | 7 | 28.3% | 0.00234 | 46852 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 13 | 20 | 27.7% | 0.00447 | 69768 |
[ |
SHM1 | Mitochondrial serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahy... | 8 | 8 | 26.1% | 0.00234 | 53686 |
[ |
GPM1 | Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... | 3 | 3 | 25.9% | 0.00174 | 27609 |
[ |
RPL31A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similari... | 2 | 2 | 23.9% | 0.00254 | 12953 |
[ |
RPL31B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similari... | 2 | 2 | 23.9% | 0.00254 | 12967 |
[ |
TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... | 5 | 8 | 22.3% | 0.00345 | 35747 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 9 | 11 | 21.7% | 0.00254 | 61987 |
[ |
RPS15 | Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S19 and rat S15 ri... | 3 | 4 | 21.1% | 0.00404 | 16002 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 9 | 16 | 20.8% | 0.00407 | 61495 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 6 | 7 | 20.6% | 0.0023 | 46914 |
[ |
HHF1, HHF2 | Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two... | 3 | 7 | 18.4% | 0.00974 | 11368 |
[ |
RPL4B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... | 3 | 4 | 17.1% | 0.00158 | 39062 |
[ |
RPL4A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 3 | 4 | 17.1% | 0.00158 | 39092 |
[ |
KRT10 | keratin 10 | 6 | 10 | 17.0% | 0.00242 | 59519 |
[ |
KRT2 | keratin 2 | 5 | 6 | 16.4% | 0.00133 | 65865 |
[ |
RPS4B, RPS4A | Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to ra... | 2 | 4 | 16.1% | 0.0022 | 29410 |
[ |
RPL8B | Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has simila... | 3 | 3 | 14.8% | 0.00168 | 28112 |
[ |
RPL8A | Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has simila... | 3 | 3 | 14.8% | 0.00168 | 28125 |
[ |
PGK1 | 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... | 4 | 4 | 14.4% | 0.00138 | 44738 |
[ |
SSA3 | ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotrans... | 8 | 10 | 13.9% | 0.00221 | 70547 |
[ |
YDJ1 | Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... | 2 | 2 | 13.4% | 7.01E-4 | 44671 |
[ |
ENO1 | Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... | 4 | 5 | 12.8% | 0.00164 | 46816 |
[ |
RPL11B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Ap; involved in rib... | 2 | 2 | 12.6% | 0.00165 | 19750 |
[ |
RPL11A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl11Bp; involved in rib... | 2 | 2 | 12.6% | 0.00165 | 19720 |
[ |
Contaminant_KERA... | 9 | 13 | 11.2% | 0.0029 | 65494 | |
[ |
IST1 | Protein with a positive role in the multivesicular body sorting pathway; functions and forms a compl... | 2 | 2 | 10.1% | 9.62E-4 | 34496 |
[ |
gi|105815 | pir||A34720 keratin 8, type II cytoskeletal - human | 4 | 5 | 8.7% | 0.00148 | 53748 |
[ |
CYS4 | Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the ... | 2 | 2 | 8.5% | 5.66E-4 | 56022 |
[ |
SAM1 | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... | 2 | 2 | 8.4% | 7.51E-4 | 41818 |
[ |
SAM2 | S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom ... | 2 | 2 | 8.3% | 7.47E-4 | 42256 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 4 | 5 | 8.2% | 0.00143 | 54545 |
[ |
YHR054C | Putative protein of unknown function | 2 | 2 | 8.2% | 8.1E-4 | 41061 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 2 | 2 | 8.1% | 6.26E-4 | 50033 |
[ |
TDH2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... | 3 | 5 | 8.1% | 0.00216 | 35847 |
[ |
SSA4 | Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational... | 6 | 9 | 7.8% | 0.00201 | 69651 |
[ |
KRT5 | keratin 5 | 4 | 5 | 7.6% | 0.00121 | 62461 |
[ |
gi|34069 | gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] | 2 | 3 | 7.6% | 0.0012 | 39219 |
[ |
GLO4 | Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and... | 2 | 2 | 7.4% | 0.00101 | 32339 |
[ |
TCF3 | transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) | 2 | 2 | 7.2% | 4.38E-4 | 67600 |
[ |
PAP2 | Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP co... | 2 | 2 | 6.7% | 4.91E-4 | 66031 |
[ |
CYS3 | Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathw... | 2 | 2 | 6.6% | 7.28E-4 | 42542 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 2 | 2 | 6.6% | 8.24E-4 | 36849 |
[ |
contaminant_KERA... | 3 | 4 | 6.4% | 0.00102 | 59822 | |
[ |
SPG5 | Protein required for survival at high temperature during stationary phase; not required for growth o... | 2 | 2 | 6.4% | 7.69E-4 | 42163 |
[ |
HSP60 | Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... | 2 | 2 | 5.4% | 5.01E-4 | 60752 |
[ |
RPL3 | Protein component of the large (60S) ribosomal subunit, has similarity to E. coli L3 and rat L3 ribo... | 2 | 2 | 5.4% | 7.41E-4 | 43758 |
[ |
contaminant_GR78... | 4 | 5 | 5.3% | 0.00108 | 73235 | |
[ |
I50731, Contamin... | owl|I50731| immunoglobulin heavy chain - nurse shark | 2 | 2 | 5.2% | 4.97E-4 | 63529 |
[ |
ADP1 | Putative ATP-dependent permease of the ABC transporter family of proteins | 2 | 3 | 4.9% | 4.1E-4 | 117231 |
[ |
KRT76 | keratin 76 | 2 | 2 | 4.9% | 4.49E-4 | 65871 |
[ |
MSC6 | Protein of unknown function; mutant is defective in directing meiotic recombination events to homolo... | 2 | 2 | 4.6% | 4.14E-4 | 79947 |
[ |
contaminant_KERA... | 2 | 3 | 4.3% | 9.19E-4 | 51203 | |
[ |
DSL1 | Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; forms a complex with Sec39p a... | 3 | 4 | 4.2% | 7.61E-4 | 88072 |
[ |
PDC5 | Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruva... | 2 | 3 | 3.9% | 7.64E-4 | 61912 |
[ |
VPS34 | Phosphatidylinositol 3-kinase responsible for the synthesis of phosphatidylinositol 3-phosphate; for... | 2 | 2 | 3.8% | 3.28E-4 | 100921 |
[ |
GR78_PLAFO, cont... | 2 | 4 | 3.8% | 8.75E-4 | 72776 | |
[ |
PIB2 | Protein binding phosphatidylinositol 3-phosphate, involved in telomere-proximal repression of gene e... | 2 | 2 | 3.8% | 4.52E-4 | 70617 |
[ |
YBP2 | Central kinetochore associated protein that mediates mitotic progression; interacts with several cen... | 2 | 3 | 3.7% | 6.71E-4 | 73102 |
[ |
Contaminant_KERA... | 2 | 3 | 3.7% | 8.05E-4 | 57265 | |
[ |
TIF4631 | Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that al... | 2 | 2 | 3.6% | 3.01E-4 | 107101 |
[ |
LHS1 | Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and fo... | 2 | 2 | 3.6% | 3.25E-4 | 99572 |
[ |
GR78_MAIZE, cont... | 2 | 3 | 3.6% | 9.21E-4 | 51689 | |
[ |
RSC30 | Component of the RSC chromatin remodeling complex; non-essential gene required for regulation of rib... | 2 | 2 | 3.5% | 3.45E-4 | 95520 |
[ |
KRT3 | keratin 3 | 2 | 5 | 3.5% | 0.00114 | 64511 |
[ |
SFB2 | Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat, required for cargo selection du... | 2 | 8 | 3.3% | 0.00131 | 98943 |
[ |
ECM10 | Heat shock protein of the Hsp70 family, localized in mitochondrial nucleoids, plays a role in protei... | 2 | 3 | 3.3% | 6.68E-4 | 70085 |
[ |
contaminant_GR78... | 2 | 3 | 3.2% | 6.58E-4 | 72379 | |
[ |
gi|109893 | pir||A37048 dnaK-type molecular chaperone grp78 precursor - mouse | 2 | 3 | 3.2% | 6.57E-4 | 72421 |
[ |
GRP78_RAT | 78 kDa glucose-regulated protein OS=Rattus norvegicus GN=Hspa5 PE=1 SV=1 | 2 | 3 | 3.2% | 6.58E-4 | 72347 |
[ |
GR78_SCHPO, cont... | 2 | 3 | 3.2% | 6.49E-4 | 73081 | |
[ |
gi|1143492, gi|8... | pir||A29821 dnaK-type molecular chaperone HSPA5 precursor - human, gi|386758|gb|AAA52614.1| GRP78 p... | 2 | 3 | 3.2% | 6.59E-4 | 72116 |
[ |
SSC1 | Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... | 2 | 2 | 3.2% | 4.38E-4 | 70628 |
[ |
contaminant_GR78... | 2 | 3 | 3.1% | 6.33E-4 | 74448 | |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 2 | 3 | 3.1% | 6.31E-4 | 74468 |
[ |
APL2 | Beta-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; invol... | 2 | 4 | 2.9% | 7.9E-4 | 81869 |
[ |
SSM4 | Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear en... | 2 | 4 | 2.6% | 4.35E-4 | 151454 |
[ |
GDE1 | Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphospha... | 2 | 20 | 2.3% | 0.00234 | 138014 |
[ |
GLT1 | NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglut... | 2 | 3 | 2.2% | 2.0E-4 | 238100 |
[ |
YLR001C | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... | 3 | 23 | 2.2% | 0.00383 | 97618 |
[ |
NUP159 | Nucleoporin, subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, ... | 2 | 2 | 2.1% | 1.96E-4 | 158907 |
[ |
YBR235W | Putative ion transporter, similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family; Y... | 2 | 2 | 2.1% | 2.56E-4 | 123998 |
[ |
MUC1 | GPI-anchored cell surface glycoprotein (flocculin) required for pseudohyphal formation, invasive gro... | 2 | 4 | 1.7% | 4.19E-4 | 136110 |
[ |
ERT1 | Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cyto... | 1 | 16 | 1.5% | 0.00434 | 60311 |
[ |
TOR2 | PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth i... | 2 | 2 | 1.4% | 1.16E-4 | 281509 |
[ |
URA2 | Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... | 2 | 2 | 1.4% | 1.3E-4 | 245123 |
[ |
MDM20 | Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of pro... | 2 | 2 | 1.1% | 3.6E-4 | 92809 |
[ |
TOM1 | E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may reg... | 2 | 4 | 1.0% | 1.75E-4 | 374186 |
[ |
BLM10 | Proteasome activator subunit; found in association with core particles, with and without the 19S reg... | 2 | 3 | 1.0% | 2.01E-4 | 245993 |
[ |
SIR1 | Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitm... | 1 | 15 | 1.0% | 0.00317 | 79929 |
[ |
RIM15 | Glucose-repressible protein kinase involved in signal transduction during cell proliferation in resp... | 1 | 13 | 0.8% | 0.00105 | 196530 |
[ |
RPA135 | RNA polymerase I second largest subunit A135 | 1 | 10 | 0.7% | 0.00119 | 135742 |
[ |
CSF1 | Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected ... | 1 | 12 | 0.4% | 5.82E-4 | 338258 |