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Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex

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[View our MS interpretation guidelines.]
  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   gi|34069 gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] 37 242 66.1% 0.064 39219
[View Peptides]   KRT10 keratin 10 80 998 62.7% 0.159 59519
[View Peptides]   Contaminant_KERA... 42 211 59.0% 0.0423 51531
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 68 377 58.7% 0.0572 61987
[View Peptides]   KRT2 keratin 2 72 600 56.3% 0.0878 65865
[View Peptides]   Contaminant_KERA... 64 739 51.3% 0.109 65494
[View Peptides]   contaminant_KERA... 39 223 50.5% 0.0375 59822
[View Peptides]   KRT17 keratin 17 31 128 49.0% 0.028 47974
[View Peptides]   KRT5 keratin 5 46 275 48.3% 0.044 62461
[View Peptides]   contaminant_KERA... 30 160 48.0% 0.0322 50499
[View Peptides]   RPS29B Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Ap and has similari... 3 3 46.4% 0.00506 6728
[View Peptides]   UBB ubiquitin B 2 6 32.9% 0.00745 8565
[View Peptides]   RPL38 Protein component of the large (60S) ribosomal subunit, has similarity to rat L38 ribosomal protein 2 4 32.1% 0.00484 8827
[View Peptides]   RPS29A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps29Bp and has similari... 2 2 32.1% 0.00337 6661
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 16 47 30.0% 0.00784 63004
[View Peptides]   RPL17B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similari... 4 9 29.3% 0.00462 20551
[View Peptides]   RPL17A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similari... 4 11 29.3% 0.00564 20549
[View Peptides]   RPL20B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Ap and has similari... 5 9 28.7% 0.00488 20713
[View Peptides]   RPL20A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl20Bp and has similari... 5 9 28.1% 0.00477 21236
[View Peptides]   RPS13 Protein component of the small (40S) ribosomal subunit; has similarity to E. coli S15 and rat S13 ri... 4 6 27.2% 0.00375 17029
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 7 27 26.5% 0.00901 32498
[View Peptides]   RPL27A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Bp and has similari... 4 7 25.7% 0.00486 15531
[View Peptides]   RPL27B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Ap and has similari... 4 7 25.7% 0.00486 15505
[View Peptides]   RPL31B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Ap and has similari... 2 4 23.9% 0.00334 12967
[View Peptides]   RPL31A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl31Bp and has similari... 2 4 23.9% 0.00334 12953
[View Peptides]   RPL32 Protein component of the large (60S) ribosomal subunit, has similarity to rat L32 ribosomal protein;... 4 4 23.8% 0.00291 14771
[View Peptides]   SPA1_STAAU (P02976) Immunoglobulin G binding protein A precursor (IgG binding protein A) (Staphylococcal protei... 13 17 23.5% 0.00306 57320
[View Peptides]   TDH3 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... 5 6 23.2% 0.00171 35747
[View Peptides]   RPL28 Ribosomal protein of the large (60S) ribosomal subunit, has similarity to E. coli L15 and rat L27a r... 4 12 22.1% 0.0076 16722
[View Peptides]   contaminant_KERA... isb|A33652|A33652|probable keratin 16, type I, pseudogene - human 18 106 21.8% 0.0212 50915
[View Peptides]   RPS17A Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Bp and has similari... 2 3 20.6% 0.00208 15788
[View Peptides]   RPS17B Ribosomal protein 51 (rp51) of the small (40s) subunit; nearly identical to Rps17Ap and has similari... 2 3 20.6% 0.00208 15803
[View Peptides]   contaminant_TRYP... null 8 16 19.7% 0.0066 23993
[View Peptides]   RPL43B, RPL43A Protein component of the large (60S) ribosomal subunit, identical to Rpl43Ap and has similarity to r... 2 2 19.6% 0.00205 10091
[View Peptides]   RPL40B, RPL40A Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of ... 2 13 19.5% 0.00959 14554
[View Peptides]   RPS16B, RPS16A Protein component of the small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E... 2 3 18.9% 0.00198 15847
[View Peptides]   MAM3 Protein required for normal mitochondrial morphology, has similarity to hemolysins 9 26 17.7% 0.00348 77712
[View Peptides]   SEH1 Nuclear pore protein that is part of the evolutionarily conserved Nup84p complex (Nup84p, Nup85p, Nu... 4 4 17.5% 0.00108 39123
[View Peptides]   RPL25 Primary rRNA-binding ribosomal protein component of the large (60S) ribosomal subunit, has similarit... 2 2 16.9% 0.00133 15758
[View Peptides]   KRT19 keratin 19 14 33 16.8% 0.00779 44106
[View Peptides]   KRT3 keratin 3 13 293 16.5% 0.044 64511
[View Peptides]   RPS31 Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... 2 13 16.4% 0.00808 17216
[View Peptides]   KRT15 keratin 15 16 78 16.2% 0.0162 49168
[View Peptides]   contaminant_INT-... 2 4 20 16.1% 0.00491 42750
[View Peptides]   contaminant_NRL_... owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... 7 13 15.9% 0.00287 46852
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 6 11 15.8% 0.00208 54545
[View Peptides]   RPL13B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... 3 3 15.1% 0.00142 22525
[View Peptides]   RPL13A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Bp; not essential f... 3 3 15.1% 0.00142 22554
[View Peptides]   TSA1 Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... 2 2 14.8% 9.64E-4 21590
[View Peptides]   RPL18A, RPL18B Protein component of the large (60S) ribosomal subunit, identical to Rpl18Bp and has similarity to r... 2 8 14.5% 0.00406 20563
[View Peptides]   NYV1 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... 3 3 14.2% 0.00112 28964
[View Peptides]   RPL19B, RPL19A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similari... 2 2 13.8% 9.99E-4 21704
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 2 4 13.4% 0.00174 24668
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 15 23 13.0% 0.00148 166749
[View Peptides]   RPL8B Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has simila... 3 4 12.1% 0.00148 28112
[View Peptides]   YBL104C Putative protein of unknown function, promoter contains multiple GCN4 binding sites 8 10 12.0% 9.1E-4 117636
[View Peptides]   PPN1 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer 6 9 11.9% 0.00126 78344
[View Peptides]   gi|105815 pir||A34720 keratin 8, type II cytoskeletal - human 9 39 11.8% 0.00762 53748
[View Peptides]   SRO9 Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulat... 3 4 11.8% 8.7E-4 48060
[View Peptides]   RPL2A, RPL2B Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E.... 4 9 11.8% 0.00335 27408
[View Peptides]   INT-STD1, Contam... 1 6 16 11.4% 0.00249 69271
[View Peptides]   RPL4A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... 3 4 11.3% 0.00104 39092
[View Peptides]   RPL4B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... 3 4 11.3% 0.00104 39062
[View Peptides]   GPM1 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... 2 2 10.9% 7.65E-4 27609
[View Peptides]   FRA1 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... 6 8 10.4% 0.00101 84924
[View Peptides]   COT1 Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistanc... 3 3 10.0% 6.45E-4 48155
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 4 8 9.8% 0.00165 50033
[View Peptides]   KRT76 keratin 76 9 116 9.6% 0.0172 65871
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 4 7 9.4% 0.00113 64382
[View Peptides]   gi|34033, gi|71526 pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... 10 77 9.2% 0.0159 49644
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 3 6 8.8% 0.00151 41690
[View Peptides]   YNL217W Hypothetical protein 3 3 8.6% 8.69E-4 37151
[View Peptides]   Contaminant_KERA... 8 83 8.4% 0.0147 57265
[View Peptides]   RPS4B, RPS4A Protein component of the small (40S) ribosomal subunit; identical to Rps4Ap and has similarity to ra... 2 2 8.4% 7.24E-4 29410
[View Peptides]   Reverse_YPL112C PEX25 SGDID:S000006033, Chr XVI from 338619-337435, reverse complement, Verified ORF, "Peripheral pe... 2 3 7.4% 7.17E-4 44911
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 2 13 6.6% 0.00322 42826
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 2 2 6.5% 4.56E-4 45407
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 2 3 6.4% 7.89E-4 39621
[View Peptides]   ZRC1 Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; a... 2 13 6.3% 0.00278 48345
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 2 2 6.3% 5.98E-4 36711
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 2 2 6.0% 5.43E-4 36849
[View Peptides]   FAA4 Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... 3 4 5.8% 5.44E-4 77267
[View Peptides]   ULA1 Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylat... 2 2 5.8% 4.09E-4 52853
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 2 2 5.7% 3.28E-4 64597
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 2 4 5.7% 0.00127 33715
[View Peptides]   gi|1143492, gi|8... pir||A29821 dnaK-type molecular chaperone HSPA5 precursor - human, gi|386758|gb|AAA52614.1| GRP78 p... 2 2 5.1% 2.89E-4 72116
[View Peptides]   gi|109893 pir||A37048 dnaK-type molecular chaperone grp78 precursor - mouse 2 2 5.0% 2.88E-4 72421
[View Peptides]   GRP78_RAT 78 kDa glucose-regulated protein OS=Rattus norvegicus GN=Hspa5 PE=1 SV=1 2 2 5.0% 2.89E-4 72347
[View Peptides]   GRP78_MESAU 78 kDa glucose-regulated protein OS=Mesocricetus auratus GN=HSPA5 PE=1 SV=1 2 2 5.0% 2.89E-4 72379
[View Peptides]   ADE5,7 Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... 3 3 4.6% 3.53E-4 86068
[View Peptides]   YIL108W Putative metalloprotease 2 2 4.6% 2.71E-4 77414
[View Peptides]   SLM1 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in ... 2 2 4.5% 2.75E-4 77995
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 2 2 4.5% 2.94E-4 69768
[View Peptides]   LRO1 Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that c... 2 3 4.4% 4.29E-4 75393
[View Peptides]   VAM6 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... 3 4 4.2% 3.6E-4 122881
[View Peptides]   MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... 4 7 4.2% 6.82E-4 109736
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 2 3 3.7% 3.26E-4 98712
[View Peptides]   UTP25 Nucleolar protein that cosediments with the 40S ribosomal subunit and is required for its biogenesis... 2 2 3.7% 2.62E-4 83991
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 4 6 3.4% 3.74E-4 171120
[View Peptides]   UBP3 Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde tran... 2 3 3.4% 3.11E-4 101917
[View Peptides]   MNN1 Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... 2 2 3.3% 2.48E-4 88529
[View Peptides]   PHO90 Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthet... 2 2 3.2% 2.14E-4 97689
[View Peptides]   VPH1 Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... 2 2 3.1% 2.25E-4 95529
[View Peptides]   VPS41 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... 2 2 3.0% 1.9E-4 113412
[View Peptides]   VAC7 Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p k... 2 3 2.7% 2.43E-4 128140
[View Peptides]   Reverse_YBR097W VPS15 SGDID:S000000301, Chr II from 436945-441309, Verified ORF, "Myristoylated serine/threonine pro... 2 2 2.5% 1.3E-4 166358
[View Peptides]   PHO91 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... 2 3 2.5% 3.17E-4 99490
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 3 3 2.1% 1.28E-4 245123
[View Peptides]   Reverse_YDL140C RPO21 SGDID:S000002299, Chr IV from 210562-205361, reverse complement, Verified ORF, "RNA polymerase... 2 2 2.0% 1.09E-4 191610
[View Peptides]   Reverse_YPL084W BRO1 SGDID:S000006005, Chr XVI from 394035-396569, Verified ORF, "Cytoplasmic class E vacuolar prote... 2 3 1.3% 3.35E-4 97276
[View Peptides]   HSL1 Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates wit... 2 6 1.2% 3.73E-4 169592
[View Peptides]   Reverse_YDR457W TOM1 SGDID:S000002865, Chr IV from 1369780-1379586, Verified ORF, "E3 ubiquitin ligase of the hect-d... 2 2 0.9% 5.78E-5 374185
[View Peptides]   Reverse_YMR231W PEP5 SGDID:S000004844, Chr XIII from 733544-736633, Verified ORF, "Peripheral vacuolar membrane prot... 2 7 0.8% 6.42E-4 117477
YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle