Bait Protein: | VAM3 |
Organism: | Saccharomyces cerevisiae |
Publication: | Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press] |
Comments: | Sample: HX13 - trans-SNARE complex forms but fusion is blocked. |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
gi|34069 | gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] | 41 | 308 | 58.0% | 0.0768 | 39219 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 85 | 708 | 53.2% | 0.101 | 61987 |
[ |
VAM3 | Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... | 13 | 116 | 52.3% | 0.0365 | 32498 |
[ |
KRT17 | keratin 17 | 30 | 249 | 50.8% | 0.0515 | 47974 |
[ |
Contaminant_KERA... | 36 | 380 | 50.5% | 0.0719 | 51531 | |
[ |
contaminant_KERA... | 36 | 404 | 47.5% | 0.0767 | 50499 | |
[ |
VAM7 | Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... | 14 | 77 | 46.5% | 0.0217 | 36711 |
[ |
KRT10 | keratin 10 | 41 | 371 | 42.8% | 0.0557 | 59519 |
[ |
contaminant_KERA... | 42 | 339 | 42.3% | 0.0537 | 59822 | |
[ |
SEC17 | Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... | 11 | 37 | 42.1% | 0.0113 | 32803 |
[ |
KRT5 | keratin 5 | 41 | 242 | 40.5% | 0.0365 | 62461 |
[ |
Contaminant_KERA... | 48 | 577 | 36.1% | 0.0799 | 65494 | |
[ |
NYV1 | v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... | 7 | 11 | 35.2% | 0.00387 | 28964 |
[ |
KRT2 | keratin 2 | 34 | 301 | 31.9% | 0.0416 | 65865 |
[ |
VTI1 | Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... | 6 | 28 | 30.0% | 0.0115 | 24668 |
[ |
contaminant_KERA... | isb|A33652|A33652|probable keratin 16, type I, pseudogene - human | 20 | 278 | 24.9% | 0.0523 | 50915 |
[ |
PDB1 | E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... | 5 | 9 | 21.9% | 0.00219 | 40054 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 8 | 17 | 21.9% | 0.00237 | 69470 |
[ |
PHO8 | Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... | 6 | 17 | 18.7% | 0.00267 | 63004 |
[ |
KRT15 | keratin 15 | 14 | 218 | 16.9% | 0.0426 | 49168 |
[ |
PDX1 | Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogena... | 3 | 6 | 16.8% | 0.0013 | 45362 |
[ |
LAT1 | Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... | 8 | 18 | 16.4% | 0.00333 | 51818 |
[ |
KRT19 | keratin 19 | 12 | 169 | 15.2% | 0.0376 | 44106 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 6 | 12 | 15.1% | 0.00166 | 69768 |
[ |
SPA_STAAU | Immunoglobulin G-binding protein A OS=Staphylococcus aureus GN=spa PE=1 SV=1 | 4 | 6 | 14.8% | 0.00105 | 55439 |
[ |
KRT3 | keratin 3 | 14 | 147 | 14.5% | 0.0208 | 64511 |
[ |
SPA1_STAAU | (P02976) Immunoglobulin G binding protein A precursor (IgG binding protein A) (Staphylococcal protei... | 4 | 6 | 14.3% | 0.00102 | 57320 |
[ |
HYP2 | Translation elongation factor eIF-5A, previously thought to function in translation initiation; simi... | 2 | 2 | 14.0% | 0.00113 | 17114 |
[ |
SEC18 | ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... | 6 | 13 | 13.9% | 0.00153 | 84056 |
[ |
HOC1 | Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localize... | 2 | 3 | 13.6% | 6.75E-4 | 46297 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 6 | 18 | 13.3% | 0.00235 | 74468 |
[ |
YPK9 | Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... | 12 | 19 | 12.6% | 0.00115 | 166749 |
[ |
KTR4 | Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltran... | 3 | 3 | 12.5% | 5.76E-4 | 54556 |
[ |
PRM5 | Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrit... | 2 | 2 | 11.9% | 5.6E-4 | 34651 |
[ |
gi|34033, gi|71526 | pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... | 9 | 109 | 11.6% | 0.0212 | 49644 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 3 | 3 | 11.4% | 5.34E-4 | 54545 |
[ |
gi|105815 | pir||A34720 keratin 8, type II cytoskeletal - human | 10 | 45 | 11.2% | 0.0083 | 53748 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 4 | 5 | 10.8% | 7.26E-4 | 66595 |
[ |
PCL1 | Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved... | 2 | 2 | 10.4% | 6.38E-4 | 32095 |
[ |
VPH1 | Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... | 5 | 6 | 9.5% | 6.36E-4 | 95529 |
[ |
CPS1 | Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions | 3 | 6 | 9.4% | 9.28E-4 | 64597 |
[ |
PDA1 | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... | 3 | 7 | 9.3% | 0.00148 | 46343 |
[ |
ENA5 | Protein with similarity to P-type ATPase sodium pumps, member of the Na+ efflux ATPase family | 5 | 6 | 9.2% | 4.9E-4 | 120296 |
[ |
ENA2 | P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li... | 5 | 6 | 9.2% | 4.9E-4 | 120317 |
[ |
ENA1 | P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance | 5 | 6 | 9.2% | 4.9E-4 | 120357 |
[ |
VPS16 | Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; par... | 4 | 4 | 9.0% | 4.46E-4 | 92741 |
[ |
RPL4A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 2 | 2 | 8.6% | 4.92E-4 | 39092 |
[ |
RPL4B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... | 2 | 2 | 8.6% | 4.92E-4 | 39062 |
[ |
ZRT3 | Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm whe... | 2 | 2 | 8.2% | 3.54E-4 | 55403 |
[ |
VBA4 | Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GF... | 3 | 5 | 8.1% | 5.8E-4 | 85683 |
[ |
VPS41 | Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... | 5 | 6 | 7.8% | 5.39E-4 | 113412 |
[ |
YCK3 | Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fus... | 2 | 3 | 7.8% | 5.1E-4 | 60261 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 7 | 13 | 7.7% | 6.17E-4 | 214849 |
[ |
KRT76 | keratin 76 | 9 | 114 | 7.1% | 0.0159 | 65871 |
[ |
IVY1 | Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... | 2 | 3 | 6.8% | 5.9E-4 | 49976 |
[ |
contaminant_NRL_... | owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... | 2 | 9 | 6.8% | 0.00187 | 46852 |
[ |
VTC3 | Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... | 4 | 17 | 6.6% | 0.00181 | 96553 |
[ |
VAM6 | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... | 4 | 6 | 6.5% | 5.09E-4 | 122881 |
[ |
PHO84 | High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... | 3 | 6 | 6.5% | 9.1E-4 | 64382 |
[ |
PDI1 | Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... | 2 | 5 | 6.5% | 8.53E-4 | 58227 |
[ |
PEP5 | Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein tra... | 4 | 6 | 6.4% | 5.19E-4 | 117477 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 2 | 3 | 6.4% | 7.12E-4 | 41690 |
[ |
KRT20 | keratin 20 | 3 | 35 | 6.1% | 0.00735 | 48487 |
[ |
YDR089W | Protein of unknown function; deletion confers resistance to Nickel | 3 | 7 | 6.0% | 7.17E-4 | 98712 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 7 | 10 | 5.6% | 5.88E-4 | 171120 |
[ |
DAP2 | Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membra... | 3 | 4 | 5.3% | 4.36E-4 | 93404 |
[ |
HXT3 | Low affinity glucose transporter of the major facilitator superfamily, expression is induced in low ... | 2 | 2 | 5.3% | 3.14E-4 | 62558 |
[ |
gi|1143492, gi|8... | pir||A29821 dnaK-type molecular chaperone HSPA5 precursor - human, gi|386758|gb|AAA52614.1| GRP78 p... | 2 | 2 | 5.2% | 2.73E-4 | 72116 |
[ |
Contaminant_KERA... | 4 | 82 | 5.2% | 0.0137 | 57265 | |
[ |
KRE6 | Protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; appears functional... | 2 | 2 | 5.0% | 2.47E-4 | 80123 |
[ |
SHM2 | Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrof... | 4 | 9 | 4.9% | 0.00171 | 52219 |
[ |
HMG1 | One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, whi... | 3 | 3 | 4.3% | 2.53E-4 | 115625 |
[ |
TFP1 | Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein prec... | 2 | 2 | 4.0% | 1.66E-4 | 118637 |
[ |
FAA4 | Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... | 2 | 3 | 4.0% | 3.85E-4 | 77267 |
[ |
Reverse_YGR092W | DBF2 SGDID:S000003324, Chr VII from 668193-669911, Verified ORF, "Ser/Thr kinase involved in transcr... | 2 | 2 | 3.7% | 3.11E-4 | 66148 |
[ |
YBT1 | Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mam... | 3 | 4 | 3.3% | 2.14E-4 | 189161 |
[ |
MNR2 | Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... | 3 | 4 | 3.2% | 3.68E-4 | 109736 |
[ |
YGR125W | Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal ... | 2 | 2 | 3.0% | 1.72E-4 | 116971 |
[ |
PUF2 | Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that ... | 2 | 2 | 2.6% | 1.66E-4 | 119508 |
[ |
PEP3 | Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... | 2 | 2 | 2.6% | 1.94E-4 | 107398 |
[ |
SEC27 | Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport... | 2 | 2 | 2.6% | 2.0E-4 | 99445 |
[ |
Reverse_YMR231W | PEP5 SGDID:S000004844, Chr XIII from 733544-736633, Verified ORF, "Peripheral vacuolar membrane prot... | 3 | 3 | 2.3% | 2.59E-4 | 117477 |
[ |
VTH2 | Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in ... | 2 | 2 | 2.1% | 1.15E-4 | 174401 |
[ |
Reverse_YER173W | RAD24 SGDID:S000000975, Chr V from 536295-538274, Verified ORF, "Checkpoint protein, involved in the... | 2 | 2 | 2.1% | 2.7E-4 | 75727 |
[ |
VTH1 | Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in ... | 2 | 2 | 2.1% | 1.15E-4 | 174427 |
[ |
Reverse_YOR086C | TCB1 SGDID:S000005612, Chr XV from 486780-483220, reverse complement, Verified ORF, "Contains three ... | 2 | 2 | 2.0% | 1.5E-4 | 133576 |
[ |
VAC7 | Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p k... | 2 | 2 | 1.6% | 1.53E-4 | 128140 |
[ |
Reverse_YDR457W | TOM1 SGDID:S000002865, Chr IV from 1369780-1379586, Verified ORF, "E3 ubiquitin ligase of the hect-d... | 2 | 3 | 0.4% | 8.17E-5 | 374185 |