Bait Protein: | VAM3 |
Organism: | Saccharomyces cerevisiae |
Publication: | Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press] |
Comments: | sample: hx4 |
Raw Data: |
Download DTASelect Filter text Download DTASelect HTML file |
UNFILTERED Run Results:
[filter] [View our MS interpretation guidelines.] |
|||||||
Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
KRT10 | keratin 10 | 40 | 100 | 51.4% | 0.0679 | 59519 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 4 | 5 | 48.1% | 0.019 | 10746 |
[ |
gi|2119236, gi|4... | sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... | 26 | 43 | 41.5% | 0.0279 | 61987 |
[ |
Contaminant_KERA... | 33 | 102 | 39.5% | 0.0639 | 65494 | |
[ |
Contaminant_KERA... | 23 | 33 | 38.2% | 0.0282 | 51531 | |
[ |
VAM3 | Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... | 10 | 36 | 36.7% | 0.0513 | 32498 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 4 | 6 | 36.4% | 0.022 | 11050 |
[ |
gi|34069 | gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] | 18 | 46 | 35.6% | 0.0519 | 39219 |
[ |
SEC17 | Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... | 10 | 21 | 34.9% | 0.029 | 32803 |
[ |
RPL30 | Protein component of the large (60S) ribosomal subunit, has similarity to rat L30 ribosomal protein;... | 2 | 2 | 34.3% | 0.00767 | 11415 |
[ |
contaminant_KERA... | 19 | 28 | 33.7% | 0.0241 | 50499 | |
[ |
KRT17 | keratin 17 | 19 | 27 | 32.9% | 0.0252 | 47974 |
[ |
UBB | ubiquitin B | 2 | 5 | 32.9% | 0.0265 | 8565 |
[ |
VTI1 | Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... | 5 | 15 | 32.3% | 0.0279 | 24668 |
[ |
KRT2 | keratin 2 | 28 | 71 | 31.0% | 0.0444 | 65865 |
[ |
RPL4A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 7 | 11 | 27.1% | 0.0122 | 39092 |
[ |
RPL4B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... | 7 | 11 | 27.1% | 0.0122 | 39062 |
[ |
contaminant_KERA... | 19 | 43 | 24.4% | 0.0308 | 59822 | |
[ |
VAM7 | Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... | 8 | 14 | 23.4% | 0.0179 | 36711 |
[ |
RPL33B | Ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Ap and has simi... | 2 | 2 | 22.4% | 0.00753 | 12168 |
[ |
RPL33A | N-terminally acetylated ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical... | 2 | 4 | 22.4% | 0.0151 | 12154 |
[ |
KRT5 | keratin 5 | 15 | 35 | 22.0% | 0.0239 | 62461 |
[ |
RPL40B, RPL40A | Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of ... | 2 | 3 | 19.5% | 0.00944 | 14554 |
[ |
RPL36A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 3 | 3 | 19.0% | 0.0121 | 11124 |
[ |
RPL36B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl36Ap and has similari... | 3 | 3 | 19.0% | 0.0121 | 11135 |
[ |
RPL13B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... | 2 | 3 | 17.6% | 0.00607 | 22525 |
[ |
RPL13A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Bp; not essential f... | 2 | 3 | 17.6% | 0.00607 | 22554 |
[ |
LYSC_LYSEN | Lysyl endopeptidase OS=Lysobacter enzymogenes PE=1 SV=1 | 15 | 26 | 16.7% | 0.0389 | 27961 |
[ |
RPS31 | Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... | 2 | 3 | 16.4% | 0.00795 | 17216 |
[ |
RPL8B | Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Ap and has simila... | 4 | 6 | 16.4% | 0.00944 | 28112 |
[ |
RPL8A | Ribosomal protein L4 of the large (60S) ribosomal subunit, nearly identical to Rpl8Bp and has simila... | 4 | 6 | 16.4% | 0.00944 | 28125 |
[ |
RPL28 | Ribosomal protein of the large (60S) ribosomal subunit, has similarity to E. coli L15 and rat L27a r... | 2 | 4 | 16.1% | 0.0108 | 16722 |
[ |
contaminant_KERA... | isb|A33652|A33652|probable keratin 16, type I, pseudogene - human | 12 | 18 | 16.1% | 0.0153 | 50915 |
[ |
RPL17B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Ap and has similari... | 2 | 2 | 15.8% | 0.00438 | 20551 |
[ |
RPL17A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similari... | 2 | 2 | 15.8% | 0.00438 | 20549 |
[ |
RPL25 | Primary rRNA-binding ribosomal protein component of the large (60S) ribosomal subunit, has similarit... | 2 | 3 | 15.5% | 0.00851 | 15758 |
[ |
IVY1 | Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... | 4 | 6 | 15.0% | 0.00534 | 49976 |
[ |
RPL6B | Protein component of the large (60S) ribosomal subunit, has similarity to Rpl6Ap and to rat L6 ribos... | 3 | 3 | 14.8% | 0.00687 | 19986 |
[ |
RPL6A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, has similarity to Rp... | 3 | 3 | 14.8% | 0.00687 | 19961 |
[ |
KRT19 | keratin 19 | 10 | 15 | 14.5% | 0.0151 | 44106 |
[ |
RPL14B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similari... | 2 | 2 | 13.8% | 0.00584 | 15153 |
[ |
RPL14A | N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... | 2 | 2 | 13.8% | 0.00584 | 15167 |
[ |
TSA1 | Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... | 2 | 3 | 12.8% | 0.00617 | 21590 |
[ |
PDB1 | E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... | 3 | 4 | 12.3% | 0.0044 | 40054 |
[ |
VMA6 | Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an elect... | 2 | 2 | 11.6% | 0.00234 | 39791 |
[ |
RPL7B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarit... | 2 | 3 | 11.1% | 0.00495 | 27696 |
[ |
RPL7A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarit... | 2 | 3 | 11.1% | 0.00495 | 27638 |
[ |
YKT6 | Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... | 2 | 2 | 11.0% | 0.00403 | 22707 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 5 | 5 | 10.5% | 0.00315 | 69470 |
[ |
INT-STD1, Contam... | 1 | 6 | 9 | 10.2% | 0.00597 | 69271 |
[ |
CPS1 | Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions | 3 | 3 | 9.5% | 0.0021 | 64597 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 4 | 7 | 9.1% | 0.00414 | 74468 |
[ |
CIC1 | Essential protein that interacts with proteasome components and has a potential role in proteasome s... | 2 | 2 | 9.0% | 0.00214 | 42530 |
[ |
PIL1 | Primary component of eisosomes, which are large immobile cell cortex structures associated with endo... | 2 | 2 | 8.0% | 0.00238 | 38349 |
[ |
PHO8 | Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... | 3 | 7 | 8.0% | 0.00498 | 63004 |
[ |
gi|34033, gi|71526 | pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... | 6 | 10 | 7.6% | 0.0088 | 49644 |
[ |
RPP0 | Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... | 3 | 5 | 7.4% | 0.00646 | 33717 |
[ |
KRT3 | keratin 3 | 6 | 33 | 7.3% | 0.0211 | 64511 |
[ |
TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... | 2 | 2 | 7.2% | 0.00243 | 35747 |
[ |
TDH2 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... | 2 | 2 | 7.2% | 0.00243 | 35847 |
[ |
VPH1 | Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... | 4 | 5 | 7.1% | 0.0024 | 95529 |
[ |
SEC18 | ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... | 3 | 3 | 6.6% | 0.00159 | 84056 |
[ |
RPL27B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Ap and has similari... | 2 | 2 | 6.6% | 0.00593 | 15505 |
[ |
RPL27A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl27Bp and has similari... | 2 | 2 | 6.6% | 0.00593 | 15531 |
[ |
UBI4 | Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... | 2 | 15 | 6.6% | 0.0159 | 42826 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 2 | 2 | 6.4% | 0.00161 | 54545 |
[ |
FRA1 | Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... | 3 | 3 | 6.3% | 0.00161 | 84924 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 3 | 3 | 5.7% | 0.00264 | 50033 |
[ |
PPN1 | Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer | 3 | 3 | 5.5% | 0.00179 | 78344 |
[ |
Contaminant_KERA... | 3 | 13 | 5.2% | 0.00981 | 57265 | |
[ |
PHO84 | High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... | 2 | 2 | 4.9% | 0.00137 | 64382 |
[ |
LYP1 | Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of c... | 2 | 3 | 4.4% | 0.00198 | 68090 |
[ |
PEP3 | Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... | 3 | 3 | 4.2% | 0.00132 | 107398 |
[ |
FAA4 | Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... | 2 | 2 | 4.0% | 0.00116 | 77267 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 4 | 5 | 3.9% | 0.00107 | 214849 |
[ |
YDR089W | Protein of unknown function; deletion confers resistance to Nickel | 2 | 2 | 3.3% | 9.27E-4 | 98712 |
[ |
YPK9 | Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... | 4 | 5 | 3.2% | 0.00137 | 166749 |
[ |
VAM6 | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... | 2 | 2 | 3.1% | 7.68E-4 | 122881 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 3 | 5 | 3.0% | 0.00133 | 171120 |
[ |
VTC3 | Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... | 2 | 2 | 2.9% | 9.65E-4 | 96553 |
[ |
PHO91 | Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... | 2 | 2 | 2.7% | 9.01E-4 | 99490 |
[ |
YDR261C-D | Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional | 3 | 4 | 2.7% | 0.001 | 181660 |
[ |
YDR316W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198526 |
[ |
YER160C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198568 |
[ |
YDR365W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198658 |
[ |
YER138C | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198561 |
[ |
YDR098C-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198594 |
[ |
YBR012W-B | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198868 |
[ |
YDR210C-D | Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... | 3 | 4 | 2.5% | 9.18E-4 | 198838 |