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Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: sample: hx3

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UNFILTERED Run Results: [filter]

[View our MS interpretation guidelines.]
  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   KRT10 keratin 10 45 127 56.5% 0.092 59519
[View Peptides]   SEC17 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... 15 26 56.2% 0.0382 32803
[View Peptides]   NYV1 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... 9 12 52.2% 0.0204 28964
[View Peptides]   gi|2119236, gi|4... sp|P35527|K1CI_HUMAN Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9), gi|545257|gb|AAC60... 33 57 49.0% 0.0394 61987
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 14 30 44.6% 0.0408 36711
[View Peptides]   gi|34069 gi|34069|emb|CAA24760.1| unnamed protein product [Homo sapiens] 14 41 38.9% 0.0493 39219
[View Peptides]   KRT17 keratin 17 17 19 37.6% 0.0189 47974
[View Peptides]   KRT2 keratin 2 31 72 36.4% 0.0479 65865
[View Peptides]   Contaminant_KERA... 28 71 33.3% 0.0474 65494
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 12 36 32.9% 0.0546 32498
[View Peptides]   KRT5 keratin 5 21 43 32.7% 0.0313 62461
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 8 20 32.3% 0.0396 24668
[View Peptides]   contaminant_KERA... 17 43 31.3% 0.0329 59822
[View Peptides]   Contaminant_KERA... 18 19 27.6% 0.0173 51531
[View Peptides]   SSA2 ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... 12 27 26.9% 0.0181 69470
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 2 2 26.4% 0.00781 11050
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 11 26 24.3% 0.0174 69768
[View Peptides]   RPL12B, RPL12A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl12Ap; rpl12a rpl12b d... 2 2 23.0% 0.00521 17823
[View Peptides]   VMA6 Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an elect... 4 6 21.4% 0.00747 39791
[View Peptides]   contaminant_KERA... 14 16 21.3% 0.0146 50499
[View Peptides]   LYSC_LYSEN Lysyl endopeptidase OS=Lysobacter enzymogenes PE=1 SV=1 25 65 20.1% 0.104 27961
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 7 8 17.0% 0.00607 63004
[View Peptides]   contaminant_KERA... isb|A33652|A33652|probable keratin 16, type I, pseudogene - human 11 17 15.2% 0.0154 50915
[View Peptides]   SEC18 ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... 6 7 13.6% 0.00397 84056
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 7 17 13.3% 0.0107 74468
[View Peptides]   PDB1 E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... 3 4 12.3% 0.00469 40054
[View Peptides]   YKT6 Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... 2 2 12.0% 0.00429 22707
[View Peptides]   SHM2 Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrof... 4 8 11.7% 0.00732 52219
[View Peptides]   IVY1 Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... 3 8 11.7% 0.00758 49976
[View Peptides]   TDH2 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tet... 3 5 11.7% 0.00647 35847
[View Peptides]   TDH3 Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... 3 5 11.7% 0.00647 35747
[View Peptides]   VPH1 Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... 7 11 11.5% 0.00562 95529
[View Peptides]   YNL058C Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... 2 3 11.4% 0.00408 35046
[View Peptides]   RPL7B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Ap and has similarit... 2 2 11.1% 0.00352 27696
[View Peptides]   RPL7A Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarit... 2 2 11.1% 0.00352 27638
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 3 3 10.6% 0.00258 54545
[View Peptides]   VBA4 Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GF... 5 6 10.4% 0.00335 85683
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 4 12 10.1% 0.00878 64382
[View Peptides]   LAT1 Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... 3 4 9.8% 0.00356 51818
[View Peptides]   gi|34033, gi|71526 pir||KRHU3 keratin 13, type I, cytoskeletal, long splice form - human, gi|34033|emb|CAA32786.1| ker... 6 6 9.6% 0.00563 49644
[View Peptides]   RPL4B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarit... 2 2 9.4% 0.00237 39062
[View Peptides]   RPL4A N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to ... 2 2 9.4% 0.00237 39092
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 5 10 9.3% 0.00494 98712
[View Peptides]   PDI1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... 3 3 9.0% 0.00247 58227
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 11 15 9.0% 0.00343 214849
[View Peptides]   PDX1 Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogena... 2 2 9.0% 0.00209 45362
[View Peptides]   SPA1_STAAU (P02976) Immunoglobulin G binding protein A precursor (IgG binding protein A) (Staphylococcal protei... 4 5 9.0% 0.0041 57320
[View Peptides]   YNL217W Hypothetical protein 2 2 8.9% 0.00263 37151
[View Peptides]   PPN1 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer 5 6 8.5% 0.00382 78344
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 3 5 8.2% 0.0035 66602
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 3 5 8.2% 0.0035 66595
[View Peptides]   PRM5 Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrit... 2 3 7.9% 0.00405 34651
[View Peptides]   NOP56 Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... 2 2 7.7% 0.0017 56864
[View Peptides]   INT-STD1, Contam... 1 4 5 7.4% 0.00354 69271
[View Peptides]   FRA1 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... 4 7 7.3% 0.00401 84924
[View Peptides]   VAM6 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... 5 7 7.2% 0.00287 122881
[View Peptides]   KRT3 keratin 3 6 15 7.2% 0.0102 64511
[View Peptides]   contaminant_TRYP... null 7 13 7.0% 0.0244 23993
[View Peptides]   DAP2 Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membra... 4 4 6.5% 0.0021 93404
[View Peptides]   CCC1 Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack ... 2 3 6.5% 0.004 34250
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 2 6 6.4% 0.0059 46914
[View Peptides]   KRT76 keratin 76 5 9 6.3% 0.00606 65871
[View Peptides]   VPS33 ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essen... 3 3 5.5% 0.00186 79271
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 2 2 5.4% 0.00149 64597
[View Peptides]   PEP3 Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... 4 4 5.3% 0.00187 107398
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 6 6 5.1% 0.00175 166749
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 5 8 4.8% 0.00227 171120
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 2 3 4.1% 0.00281 50033
[View Peptides]   Contaminant_KERA... 3 7 3.9% 0.00563 57265
[View Peptides]   LYP1 Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of c... 2 2 3.8% 0.00141 68090
[View Peptides]   PHO91 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... 3 3 3.6% 0.00144 99490
[View Peptides]   FAA4 Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... 2 2 3.6% 0.00124 77267
[View Peptides]   contaminant_NRL_... owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... 2 2 3.5% 0.00201 46852
[View Peptides]   MNN1 Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... 2 2 3.5% 0.00113 88529
[View Peptides]   VPS16 Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; par... 3 3 3.5% 0.00161 92741
[View Peptides]   VPS41 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... 3 4 3.5% 0.00173 113412
[View Peptides]   PEP5 Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein tra... 3 3 3.0% 0.00125 117477
[View Peptides]   VTC3 Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... 2 2 2.9% 0.00103 96553
[View Peptides]   YLR241W Putative protein of unknown function, may be involved in detoxification 2 3 2.8% 0.00165 89344
[View Peptides]   PHO90 Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthet... 2 2 2.7% 9.75E-4 97689
[View Peptides]   PMC1 Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activ... 2 2 2.0% 7.32E-4 130861
[View Peptides]   PEP1 Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golg... 2 2 1.9% 5.44E-4 177777
[View Peptides]   YBT1 Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mam... 2 4 1.8% 0.00103 189161
[View Peptides]   MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... 2 2 1.5% 8.86E-4 109736
[View Peptides]   Reverse_YPL084W BRO1 SGDID:S000006005, Chr XVI from 394035-396569, Verified ORF, "Cytoplasmic class E vacuolar prote... 2 3 1.3% 0.00152 97276
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