| Bait Protein: | ASF1 |
| Hit Protein: |
|
| Protein Desc: | Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autopho... |
| Sequence Coverage: | 75.8% |
| Organism: | Saccharomyces cerevisiae |
| MS Run: | View Run |
| Sequence Coverage: |
|
| Hit Protein Sequence: [NCBI BLAST] [ProtParam] |
1 11 21 31 41 51
| | | | | |
1 MENITQPTQQ STQATQRFLI EKFSQEQIGE NIVCRVICTT GQIPIRDLSA DISQVLKEKR 60
61 SIKKVWTFGR NPACDYHLGN ISRLSNKHFQ ILLGEDGNLL LNDISTNGTW LNGQKVEKNS 120
121 NQLLSQGDEI TVGVGVESDI LSLVIFINDK FKQCLEQNKV DRIRSNLKNT SKIASPGLTS 180
181 STASSMVANK TGIFKDFSII DEVVGQGAFA TVKKAIERTT GKTFAVKIIS KRKVIGNMDG 240
241 VTRELEVLQK LNHPRIVRLK GFYEDTESYY MVMEFVSGGD LMDFVAAHGA VGEDAGREIS 300
301 RQILTAIKYI HSMGISHRDL KPDNILIEQD DPVLVKITDF GLAKVQGNGS FMKTFCGTLA 360
361 YVAPEVIRGK DTSVSPDEYE ERNEYSSLVD MWSMGCLVYV ILTGHLPFSG STQDQLYKQI 420
421 GRGSYHEGPL KDFRISEEAR DFIDSLLQVD PNNRSTAAKA LNHPWIKMSP LGSQSYGDFS 480
481 QISLSQSLSQ QKLLENMDDA QYEFVKAQRK LQMEQQLQEQ DQEDQDGKIQ GFKIPAHAPI 540
541 RYTQPKSIEA ETREQKLLHS NNTENVKSSK KKGNGRFLTL KPLPDSIIQE SLEIQQGVNP 600
601 FFIGRSEDCN CKIEDNRLSR VHCFIFKKRH AVGKSMYESP AQGLDDIWYC HTGTNVSYLN 660
661 NNRMIQGTKF LLQDGDEIKI IWDKNNKFVI GFKVEINDTT GLFNEGLGML QEQRVVLKQT 720
721 AEEKDLVKKL TQMMAAQRAN QPSASSSSMS AKKPPVSDTN NNGNNSVLND LVESPINANT 780
781 GNILKRIHSV SLSQSQIDPS KKVKRAKLDQ TSKGPENLQF S |
| Run Result Peptides: |
|||||||||
| XCorr | Delta CN | M+H+ | Total Intensity |
Sp Rank |
Ion Prop. |
Red. | Sequence | ||
| [No Spectrum] | 3.8744 | 0.2599 | 3767.45 | 9566.9 | 1 | 25.0 | 1 | I.TQPTQQSTQATQRFLIEKFSQEQIGENIVCRV.I | |
| [No Spectrum] | 4.6293 | 0.3198 | 3338.63 | 11082.3 | 1 | 28.7 | 1 | T.QQSTQATQRFLIEKFSQEQIGENIVCRV.I | |
| [No Spectrum] | 3.8871 | 0.1858 | 3453.59 | 10442.0 | 1 | 26.8 | 1 | T.QQSTQATQRFLIEKFSQEQIGENIVCRVI.C | |
| [No Spectrum] | 4.3579 | 0.3043 | 3714.23 | 9767.3 | 1 | 19.2 | 1 | T.QQSTQATQRFLIEKFSQEQIGENIVCRVICT.T | |
| [No Spectrum] | 5.6431 | 0.4962 | 3321.53 | 7959.2 | 1 | 32.0 | 1 | Q.S*T*QAT*QRFLIEKFSQEQIGENIVCRV.I | |
| [No Spectrum] | 5.2573 | 0.4601 | 3698.03 | 8104.5 | 1 | 22.3 | 2 | Q.S*T*QAT*QRFLIEKFSQEQIGENIVCRVICT.T | |
| [No Spectrum] | 4.3797 | 0.5633 | 1580.45 | 5862.7 | 1 | 70.8 | 2 | K.FSQEQIGENIVCR.V | |
| [No Spectrum] | 2.585 | 0.1361 | 1908.25 | 7663.6 | 1 | 40.0 | 1 | F.SQEQIGENIVCRVICT.T | |
| [No Spectrum] | 4.3515 | 0.507 | 1189.23 | 6802.1 | 1 | 85.0 | 2 | R.DLSADISQVLK.E | |
| [No Spectrum] | 2.6336 | 0.4309 | 1189.54 | 9063.6 | 1 | 70.0 | 1 | R.DLSADISQVLK.E | |
| [No Spectrum] | 1.8284 | 0.3811 | 960.62 | 8207.7 | 4 | 56.2 | 1 | L.SADISQVLK.E | |
| [No Spectrum] | 3.5816 | 0.6213 | 1517.83 | 10759.9 | 1 | 70.8 | 1 | R.NPACDYHLGNISR.L | |
| [No Spectrum] | 5.1851 | 0.4575 | 3556.94 | 9168.9 | 1 | 29.0 | 1 | R.LSNKHFQILLGEDGNLLLNDISTNGTWLNGQK.V | |
| [No Spectrum] | 6.103 | 0.4466 | 3110.99 | 10027.5 | 1 | 46.3 | 3 | K.HFQILLGEDGNLLLNDISTNGTWLNGQK.V | |
| [No Spectrum] | 7.1838 | 0.5212 | 3112.46 | 10185.6 | 1 | 36.1 | 63 | K.HFQILLGEDGNLLLNDISTNGTWLNGQK.V | |
| [No Spectrum] | 3.5988 | 0.2777 | 3418.22 | 9335.4 | 17 | 19.4 | 1 | K.NSNQLLSQGDEITVGVGVESDILSLVIFINDK.F | |
| [No Spectrum] | 3.4016 | 0.3015 | 1505.71 | 4878.4 | 1 | 60.7 | 6 | Q.GDEITVGVGVESDIL.S | |
| [No Spectrum] | 2.852 | 0.2037 | 1708.55 | 5488.5 | 1 | 50.0 | 7 | D.EITVGVGVES*DILSLV.I | |
| [No Spectrum] | 4.9458 | 0.5192 | 2408.77 | 10677.8 | 1 | 50.0 | 1 | V.IFINDKFKQCLEQNKVDRI.R | |
| [No Spectrum] | 5.9862 | 0.4192 | 2410.7 | 8143.1 | 1 | 45.8 | 2 | V.IFINDKFKQCLEQNKVDRI.R | |
| [No Spectrum] | 2.6377 | 0.2065 | 2197.25 | 5689.8 | 1 | 30.0 | 1 | N.T*S*KIASPGLTSSTASSMVANK.T | |
| [No Spectrum] | 5.7954 | 0.6371 | 1722.25 | 6746.9 | 1 | 73.5 | 8 | K.IASPGLTSSTASSMVANK.T | |
| [No Spectrum] | 3.7595 | 0.2957 | 1802.71 | 7200.4 | 1 | 64.7 | 1 | K.IAS*PGLTSSTASSMVANK.T | |
| [No Spectrum] | 6.2294 | 0.5344 | 2444.07 | 10003.6 | 1 | 59.1 | 3 | K.TGIFKDFSIIDEVVGQGAFATVK.K | |
| [No Spectrum] | 5.9584 | 0.4967 | 2444.63 | 6400.7 | 1 | 38.6 | 2 | K.TGIFKDFSIIDEVVGQGAFATVK.K | |
| [No Spectrum] | 3.935 | 0.4882 | 2014.81 | 8043.4 | 1 | 44.4 | 1 | T.GIFKDFSIIDEVVGQGAFA.T | |
| [No Spectrum] | 4.5012 | 0.4726 | 2114.41 | 7561.9 | 1 | 52.6 | 2 | T.GIFKDFSIIDEVVGQGAFAT.V | |
| [No Spectrum] | 3.5334 | 0.4951 | 2216.21 | 6527.3 | 1 | 40.0 | 1 | T.GIFKDFSIIDEVVGQGAFATV.K | |
| [No Spectrum] | 6.6717 | 0.6889 | 1896.21 | 8748.2 | 1 | 79.4 | 17 | K.DFSIIDEVVGQGAFATVK.K | |
| [No Spectrum] | 5.0524 | 0.5257 | 2024.65 | 7558.7 | 1 | 63.9 | 5 | K.DFSIIDEVVGQGAFATVKK.A | |
| [No Spectrum] | 2.5776 | 0.3821 | 1320.53 | 7326.6 | 1 | 50.0 | 2 | I.DEVVGQGAFATVK.K | |
| [No Spectrum] | 6.1439 | 0.4348 | 4024.31 | 8900.6 | 1 | 26.4 | 21 | K.GFYEDTESYYMVMEFVSGGDLMDFVAAHGAVGEDAGR.E | |
| [No Spectrum] | 4.8193 | 0.6361 | 2408.63 | 9071.1 | 1 | 50.0 | 2 | M.EFVSGGDLMDFVAAHGAVGEDAGR.E | |
| [No Spectrum] | 3.6941 | 0.2777 | 2409.77 | 5490.8 | 1 | 30.4 | 1 | M.EFVSGGDLMDFVAAHGAVGEDAGR.E | |
| [No Spectrum] | 3.1797 | 0.3665 | 2052.05 | 6551.2 | 1 | 36.8 | 1 | V.AAHGAVGEDAGREISRQILT.A | |
| [No Spectrum] | 3.4389 | 0.4814 | 2235.73 | 5323.9 | 1 | 40.5 | 1 | V.AAHGAVGEDAGREISRQILTAI.K | |
| [No Spectrum] | 2.9197 | 0.3394 | 1808.23 | 7077.8 | 1 | 46.9 | 1 | A.HGAVGEDAGREISRQIL.T | |
| [No Spectrum] | 2.7977 | 0.4315 | 1909.99 | 6107.1 | 2 | 38.2 | 1 | A.HGAVGEDAGREISRQILT.A | |
| [No Spectrum] | 4.027 | 0.5946 | 2096.27 | 4710.7 | 1 | 39.5 | 1 | A.HGAVGEDAGREISRQILTAI.K | |
| [No Spectrum] | 2.0318 | 0.4402 | 1200.47 | 6202.9 | 1 | 61.1 | 1 | K.YIHSMGISHR.D | |
| [No Spectrum] | 2.9813 | 0.4248 | 1721.13 | 6331.3 | 1 | 53.6 | 1 | M.GISHRDLKPDNILIE.Q | |
| [No Spectrum] | 4.6043 | 0.3809 | 2615.84 | 7497.0 | 1 | 36.4 | 1 | M.GISHRDLKPDNILIEQDDPVLVK.I | |
| [No Spectrum] | 4.0454 | 0.5717 | 2316.95 | 7399.9 | 1 | 50.0 | 4 | I.SHRDLKPDNILIEQDDPVLV.K | |
| [No Spectrum] | 3.7123 | 0.4765 | 2317.52 | 3623.4 | 1 | 34.2 | 1 | I.SHRDLKPDNILIEQDDPVLV.K | |
| [No Spectrum] | 5.1847 | 0.4284 | 2067.49 | 7522.3 | 1 | 67.6 | 2 | R.DLKPDNILIEQDDPVLVK.I | |
| [No Spectrum] | 4.5202 | 0.485 | 1697.67 | 7990.2 | 1 | 64.3 | 4 | K.TFCGTLAYVAPEVIR.G | |
| [No Spectrum] | 3.538 | 0.3957 | 3267.92 | 7066.6 | 1 | 22.2 | 2 | I.RGKDTSVSPDEYEERNEYSSLVDMWSMG.C | |
| [No Spectrum] | 4.2965 | 0.4079 | 3541.34 | 6541.8 | 1 | 25.0 | 2 | I.RGKDTSVSPDEYEERNEYSSLVDMWSMGCL.V | |
| [No Spectrum] | 4.3955 | 0.414 | 3640.61 | 7250.4 | 1 | 25.8 | 2 | I.RGKDTSVSPDEYEERNEYSSLVDMWSMGCLV.Y | |
| [No Spectrum] | 3.8435 | 0.0417 | 3646.19 | 7092.4 | 6 | 22.4 | 1 | R.GKDTS*VS*PDEYEERNEYSSLVDMWSMGCLV.Y | |
| [No Spectrum] | 4.9347 | 0.551 | 2121.55 | 8073.9 | 1 | 52.9 | 1 | K.DTSVSPDEYEERNEYSSL.V | |
| [No Spectrum] | 3.5686 | 0.4328 | 1406.67 | 4632.3 | 1 | 72.7 | 1 | R.GSYHEGPLKDFR.I | |
| [No Spectrum] | 3.9973 | 0.1619 | 3993.41 | 4872.6 | 1 | 22.8 | 1 | G.SYHEGPLKDFRISEEARDFIDSLLQVDPNNRSTAA.K | |
| [No Spectrum] | 4.4749 | 0.5034 | 1646.51 | 5496.6 | 1 | 84.6 | 5 | R.DFIDSLLQVDPNNR.S | |
| [No Spectrum] | 4.4636 | 0.4979 | 1834.55 | 6050.5 | 1 | 66.7 | 2 | R.DFIDSLLQVDPNNRST.A | |
| [No Spectrum] | 4.2277 | 0.5529 | 1976.61 | 5094.6 | 1 | 64.7 | 3 | R.DFIDSLLQVDPNNRSTAA.K | |
| [No Spectrum] | 1.9143 | 0.3482 | 980.08 | 5467.3 | 3 | 57.1 | 1 | K.ALNHPWIK.M | |
| [No Spectrum] | 4.7851 | 0.5483 | 2704.11 | 7657.2 | 1 | 47.9 | 12 | K.MSPLGSQSYGDFSQISLSQSLSQQK.L | |
| [No Spectrum] | 4.6613 | 0.4363 | 2704.73 | 12257.6 | 1 | 32.3 | 5 | K.MSPLGSQSYGDFSQISLSQSLSQQK.L | |
| [No Spectrum] | 5.151 | 0.0435 | 2785.07 | 12168.9 | 2 | 33.3 | 1 | K.MSPLGSQSY*GDFSQISLSQSLSQQK.L | |
| [No Spectrum] | 4.6246 | 0.1174 | 2785.7 | 10143.6 | 1 | 31.2 | 1 | K.MSPLGSQSYGDFSQISLS*QSLSQQK.L | |
| [No Spectrum] | 3.8037 | 0.3347 | 2574.87 | 7265.7 | 1 | 37.0 | 1 | M.SPLGSQSYGDFSQISLSQSLSQQK.L | |
| [No Spectrum] | 3.0353 | 0.0475 | 2471.89 | 9347.5 | 1 | 33.3 | 1 | P.LGSQS*YGDFSQISLSQSLSQQK.L | |
| [No Spectrum] | 4.6679 | 0.5999 | 2275.93 | 6029.2 | 1 | 50.0 | 1 | L.GSQSYGDFSQISLSQSLSQQK.L | |
| [No Spectrum] | 4.6949 | 0.5884 | 2004.77 | 5623.5 | 1 | 61.8 | 2 | Q.SYGDFSQISLSQSLSQQK.L | |
| [No Spectrum] | 2.0366 | 0.2642 | 1105.59 | 7773.4 | 4 | 55.6 | 1 | I.SLSQSLSQQK.L | |
| [No Spectrum] | 3.9967 | 0.4857 | 2674.45 | 7701.3 | 1 | 45.5 | 1 | I.SLSQSLSQQKLLENMDDAQYEFV.K | |
| [No Spectrum] | 2.1501 | 0.3752 | 905.63 | 6291.4 | 1 | 78.6 | 1 | L.SQSLSQQK.L | |
| [No Spectrum] | 4.1622 | 0.4904 | 2387.49 | 12137.7 | 1 | 47.4 | 2 | S.QSLSQQKLLENMDDAQYEFV.K | |
| [No Spectrum] | 2.967 | 0.4337 | 1715.59 | 8256.9 | 1 | 42.3 | 1 | Q.KLLENMDDAQYEFV.K | |
| [No Spectrum] | 5.5531 | 0.6021 | 1843.95 | 10098.5 | 1 | 82.1 | 3 | Q.KLLENMDDAQYEFVK.A | |
| [No Spectrum] | 3.7663 | 0.5882 | 1914.67 | 8890.0 | 1 | 53.3 | 1 | Q.KLLENMDDAQYEFVKA.Q | |
| [No Spectrum] | 5.3169 | 0.5079 | 1716.97 | 7331.7 | 1 | 80.8 | 5 | K.LLENMDDAQYEFVK.A | |
| [No Spectrum] | 4.848 | 0.1118 | 3048.8 | 10267.2 | 3 | 26.0 | 1 | A.QRKLQMEQQLQEQDQEDQDGKIQGF.K | |
| [No Spectrum] | 3.6762 | 0.3045 | 2717.21 | 9850.2 | 1 | 26.2 | 1 | Q.RKLQMEQQLQEQDQEDQDGKIQ.G | |
| [No Spectrum] | 5.5356 | 0.5286 | 2318.91 | 9986.2 | 1 | 61.1 | 8 | R.KLQMEQQLQEQDQEDQDGK.I | |
| [No Spectrum] | 7.0357 | 0.5092 | 2894.03 | 11999.6 | 1 | 38.0 | 2 | R.KLQMEQQLQEQDQEDQDGKIQGFK.I | |
| [No Spectrum] | 6.4883 | 0.3638 | 2190.15 | 9823.6 | 1 | 67.6 | 10 | K.LQMEQQLQEQDQEDQDGK.I | |
| [No Spectrum] | 5.7185 | 0.1994 | 2191.79 | 10788.2 | 1 | 39.7 | 1 | K.LQMEQQLQEQDQEDQDGK.I | |
| [No Spectrum] | 3.1073 | 0.4086 | 2320.69 | 12010.0 | 1 | 44.4 | 1 | L.QMEQQLQEQDQEDQDGKIQ.G | |
| [No Spectrum] | 3.4874 | 0.5445 | 1654.63 | 8190.0 | 1 | 65.4 | 1 | R.EQKLLHSNNTENVK.S | |
| [No Spectrum] | 2.8732 | 0.5864 | 1269.19 | 5252.2 | 1 | 75.0 | 1 | K.LLHSNNTENVK.S | |
| [No Spectrum] | 3.7087 | 0.4405 | 1571.31 | 8428.0 | 1 | 65.4 | 1 | K.LLHSNNTENVKSSK.K | |
| [No Spectrum] | 5.0714 | 0.4795 | 1908.02 | 8668.0 | 1 | 46.9 | 2 | N.NTENVKSSKKKGNGRFL.T | |
| [No Spectrum] | 4.0577 | 0.5796 | 1908.07 | 9606.4 | 1 | 68.8 | 6 | N.NTENVKSSKKKGNGRFL.T | |
| [No Spectrum] | 4.0273 | 0.3452 | 2828.97 | 9194.0 | 1 | 35.4 | 2 | R.FLTLKPLPDSIIQESLEIQQGVNPF.F | |
| [No Spectrum] | 4.8767 | 0.5427 | 3301.61 | 6795.1 | 1 | 25.9 | 4 | R.FLTLKPLPDSIIQESLEIQQGVNPFFIGR.S | |
| [No Spectrum] | 5.023 | 0.4746 | 3407.12 | 6033.2 | 1 | 30.4 | 13 | K.SMYESPAQGLDDIWYCHTGTNVSYLNNNR.M | |
| [No Spectrum] | 3.4302 | 0.3645 | 1178.47 | 4423.7 | 1 | 88.9 | 1 | K.FLLQDGDEIK.I | |
| [No Spectrum] | 3.3635 | 0.4912 | 1368.19 | 7787.6 | 1 | 75.0 | 2 | I.WDKNNKFVIGF.K | |
| [No Spectrum] | 5.8996 | 0.5826 | 2364.91 | 8799.5 | 1 | 67.5 | 4 | K.VEINDTTGLFNEGLGMLQEQR.V | |
| [No Spectrum] | 4.3077 | 0.458 | 1744.15 | 7860.5 | 1 | 71.4 | 1 | V.VLKQTAEEKDLVKKL.T | |
| [No Spectrum] | 4.145 | 0.3818 | 1645.11 | 7678.4 | 1 | 65.4 | 1 | V.LKQTAEEKDLVKKL.T | |
| [No Spectrum] | 6.0134 | 0.4901 | 4412.51 | 6729.6 | 1 | 22.6 | 1 | S.SSSMSAKKPPVSDTNNNGNNSVLNDLVESPINANTGNILKRI.H | |
| [No Spectrum] | 5.006 | 0.4349 | 3465.29 | 7419.0 | 1 | 22.7 | 3 | K.KPPVSDTNNNGNNSVLNDLVESPINANTGNILK.R | |
| [No Spectrum] | 5.0544 | 0.1 | 3544.76 | 9796.6 | 1 | 28.9 | 2 | K.KPPVSDTNNNGNNSVLNDLVES*PINANTGNILK.R | |
| [No Spectrum] | 5.3604 | 0.4088 | 3620.48 | 8947.3 | 1 | 28.8 | 1 | K.KPPVSDTNNNGNNSVLNDLVESPINANTGNILKR.I | |
| [No Spectrum] | 3.7392 | 0.2371 | 3699.56 | 5101.1 | 3 | 18.9 | 1 | K.KPPVSDTNNNGNNSVLNDLVES*PINANTGNILKR.I | |
| [No Spectrum] | 4.8381 | 0.5014 | 3310.67 | 11012.6 | 1 | 25.0 | 1 | V.SDTNNNGNNSVLNDLVESPINANTGNILKRI.H | |
| [No Spectrum] | 3.643 | 0.0912 | 3116.27 | 11744.7 | 1 | 28.7 | 1 | S.DTNNNGNNS*VLNDLVESPINANT*GNILK.R | |
| [No Spectrum] | 3.1938 | 0.4091 | 1910.85 | 3309.1 | 2 | 56.2 | 1 | K.RIHSVSLSQSQIDPSKK.V | |
| [No Spectrum] | 4.5975 | 0.5998 | 1626.61 | 7962.4 | 1 | 82.1 | 1 | R.IHSVSLSQSQIDPSK.K | |
| [No Spectrum] | 4.844 | 0.6729 | 1754.83 | 5810.6 | 1 | 76.7 | 1 | R.IHSVSLSQSQIDPSKK.V | |
| [No Spectrum] | 4.3208 | 0.6103 | 2096.17 | 4046.3 | 1 | 55.6 | 2 | I.HSVSLSQSQIDPSKKVKRA.K | |
| [No Spectrum] | 3.8561 | 0.4513 | 1772.61 | 3460.5 | 1 | 53.3 | 1 | V.SLSQSQIDPSKKVKRA.K | |
| [No Spectrum] | 3.6589 | 0.4936 | 2884.83 | 4854.8 | 1 | 34.0 | 1 | L.SQSQIDPSKKVKRAKLDQTSKGPENL.Q | |
| [No Spectrum] | 3.145 | 0.4395 | 1485.67 | 4682.2 | 1 | 62.5 | 1 | S.QSQIDPSKKVKRA.K | |
| [No Spectrum] | 3.087 | 0.3776 | 1357.75 | 5238.7 | 1 | 77.3 | 1 | Q.SQIDPSKKVKRA.K | |