| * | STY | 80.0 |
| # | X | 0.0 |
| @ | X | 0.0 |
| Static | C | 57.0 |
| true | Use criteria |
| 0.0 | Minimum peptide confidence |
| 0.05 | Peptide false positive rate |
| 0.0 | Minimum protein confidence |
| 1.0 | Protein false positive rate |
| 1 | Minimum charge state |
| 9 | Maximum charge state |
| 0.0 | Minimum ion proportion |
| 1000 | Maximum Sp rank |
| -1.0 | Minimum Sp score |
| Include | Modified peptide inclusion |
| Any | Tryptic status requirement |
| false | Multiple, ambiguous IDs allowed |
| Ignore | Peptide validation handling |
| XCorr | Purge duplicate peptides by protein |
| false | Include only loci with unique peptide |
| true | Remove subset proteins |
| Ignore | Locus validation handling |
| 0 | Minimum modified peptides per locus |
| 1000 | Minimum redundancy for low coverage loci |
| 2 | Minimum peptides per locus |
| Validation Status | Locus | Sequence Count | Spectrum Count | Sequence Coverage | Length | MolWt | pI | Descriptive Name |
| Locus | # of identical peptides | # of differing peptides |
| U | YKL089W | 64 | 224 | 52.8% | 549 | 62473 | 4.8 | MIF2 SGDID:S000001572, Chr XI from 273038-274687, Verified ORF, "Kinetochore protein with homology to human CENP-C, required for structural integrity of the spindle during anaphase spindle elongation, interacts with histones H2A, H2B, and H4, phosphorylated by Ipl1p" |
| U | YBL003C | 2 | 5 | 22.0% | 132 | 13989 | 10.7 | HTA2 SGDID:S000000099, Chr II from 235795-235397, reverse complement, Verified ORF, "One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " |
| U | YDR225W | 2 | 5 | 22.0% | 132 | 13989 | 10.7 | HTA1 SGDID:S000002633, Chr IV from 915524-915922, Verified ORF, "One of two nearly identical (see also HTA2) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " |
| Filename | XCorr | DeltCN | Conf% | ObsM+H+ | CalcM+H+ | SpR | ZScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mif2_01_itms.35487.35487.2 | 5.6406 | 0.4463 | 100.0% | 2946.7122 | 2947.4014 | 1 | 8.404 | 46.4% | 1 | R.IGSGAPVYLTAVLEYLAAEILELAGNAAR.D | 2 | |
| Mif2_01_itms.35576.35576.3 | 6.2866 | 0.3901 | 100.0% | 2948.4543 | 2947.4014 | 1 | 7.353 | 33.9% | 4 | R.IGSGAPVYLTAVLEYLAAEILELAGNAAR.D | 3 |
| U | contaminant_KERATIN02 | 6 | 17 | 17.0% | 622 | 61987 | 5.2 | no description |
| Filename | XCorr | DeltCN | Conf% | ObsM+H+ | CalcM+H+ | SpR | ZScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| * | Mif2_01_itms.01166.01166.2 | 4.7644 | 0.5181 | 100.0% | 1232.3322 | 1233.2833 | 1 | 9.541 | 86.7% | 2 | T.SGGGGGGGLGSGGSIR.S | 2 |
| * | Mif2_01_itms.01304.01304.2 | 3.9885 | 0.3855 | 100.0% | 1146.0922 | 1146.2052 | 1 | 7.246 | 82.1% | 3 | S.GGGGGGGLGSGGSIR.S | 2 |
| * | Mif2_01_itms.00524.00524.2 | 3.3562 | 0.3088 | 100.0% | 1236.8322 | 1236.2401 | 1 | 6.42 | 75.0% | 6 | R.FSSSSGYGGGSSR.V | 2 |
| * | Mif2_01_itms.12879.12879.3 | 3.9039 | 0.3297 | 100.0% | 2905.4043 | 2904.1597 | 1 | 5.849 | 34.4% | 1 | K.NYSPYYNTIDDLKDQIVDLTVGNNK.T | 3 |
| * | Mif2_01_itms.08972.08972.3 | 6.4742 | 0.4088 | 100.0% | 3266.5144 | 3266.413 | 1 | 7.717 | 33.9% | 4 | K.DIENQYETQITQIEHEVSSSGQEVQSSAK.E | 3 |
| * | Mif2_01_itms.00305.00305.2 | 5.127 | 0.2947 | 100.0% | 1794.4521 | 1792.7324 | 1 | 8.268 | 54.5% | 1 | R.GGSGGSYGGGGSGGGYGGGSGSR.G | 2 |
| U | YKL049C | 3 | 9 | 13.5% | 229 | 26841 | 9.3 | CSE4 SGDID:S000001532, Chr XI from 346408-345719, reverse complement, Verified ORF, "Centromere protein that resembles histones, required for proper kinetochore function; homolog of human CENP-A" |
| Filename | XCorr | DeltCN | Conf% | ObsM+H+ | CalcM+H+ | SpR | ZScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| * | Mif2_01_itms.03731.03731.3 | 4.645 | 0.3276 | 100.0% | 1990.4944 | 1988.1245 | 1 | 5.863 | 51.5% | 2 | R.SLSNVNRLAGDQQSINDR.A | 3 |
| * | Mif2_01_itms.00812.00812.2 | 3.6642 | 0.3161 | 100.0% | 1217.1522 | 1217.281 | 1 | 6.395 | 85.0% | 6 | R.LAGDQQSINDR.A | 2 |
| * | Mif2_01_itms.05877.05877.2 | 3.3138 | 0.3615 | 100.0% | 1463.6721 | 1461.7446 | 4 | 5.969 | 62.5% | 1 | R.STDLLISKIPFAR.L | 2 |
| U | contaminant_gi|67568|pir||ELPG | 4 | 11 | 12.8% | 266 | 28821 | 8.1 | pancreatic elastase (EC 3.4.21.36) I precursor - pig |
| Filename | XCorr | DeltCN | Conf% | ObsM+H+ | CalcM+H+ | SpR | ZScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| * | Mif2_01_itms.06416.06416.2 | 4.3422 | 0.2418 | 100.0% | 2122.652 | 2120.3257 | 1 | 5.879 | 66.7% | 5 | K.IVVHPYWNTDDVAAGYDIA.L | 2 |
| * | Mif2_01_itms.05744.05744.2 | 3.8963 | 0.2433 | 100.0% | 2007.5122 | 2007.1663 | 1 | 5.893 | 55.9% | 2 | I.VVHPYWNTDDVAAGYDIA.L | 2 |
| * | Mif2_01_itms.12246.12246.2 | 3.1636 | 0.3351 | 100.0% | 1539.1921 | 1537.7557 | 1 | 6.548 | 65.4% | 1 | R.VSAYISWINNVIAS.N | 2 |
| * | Mif2_01_itms.12131.12131.2 | 4.7547 | 0.3144 | 100.0% | 1652.7322 | 1651.8595 | 1 | 7.123 | 75.0% | 3 | R.VSAYISWINNVIASN.- | 2 |
| U | contaminant_gi|135017|sp|P07518|SUBT_BAC | 4 | 8 | 7.6% | 275 | 27656 | 6.8 | SUBTILISIN (ALKALINE MESENTERICOPEPTIDASE) |
| Filename | XCorr | DeltCN | Conf% | ObsM+H+ | CalcM+H+ | SpR | ZScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| * | Mif2_01_itms.06428.06428.2 | 4.5495 | 0.4445 | 100.0% | 2045.4722 | 2045.3867 | 1 | 7.27 | 55.0% | 3 | A.ALNNSIGVLGVAPSSALYAVK.V | 2 |
| * | Mif2_01_itms.05606.05606.2 | 4.1908 | 0.3692 | 100.0% | 1862.3322 | 1861.1484 | 1 | 5.995 | 58.3% | 2 | L.NNSIGVLGVAPSSALYAVK.V | 2 |
| * | Mif2_01_itms.05638.05638.3 | 4.543 | 0.3064 | 100.0% | 1863.3243 | 1861.1484 | 1 | 5.154 | 44.4% | 2 | L.NNSIGVLGVAPSSALYAVK.V | 3 |
| * | Mif2_01_itms.03398.03398.2 | 2.9009 | 0.3841 | 100.0% | 1165.2122 | 1163.3593 | 1 | 5.827 | 68.2% | 1 | L.GVAPSSALYAVK.V | 2 |
| U | contaminant_KERATIN03 | 2 | 3 | 6.9% | 593 | 59519 | 5.2 | no description |
| Filename | XCorr | DeltCN | Conf% | ObsM+H+ | CalcM+H+ | SpR | ZScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| * | Mif2_01_itms.11823.11823.3 | 4.9663 | 0.3124 | 100.0% | 2875.3442 | 2874.2134 | 1 | 5.777 | 30.8% | 2 | R.NVSTGDVNVEMNAAPGVDLTQLLNNMR.S | 3 |
| * | Mif2_01_itms.02176.02176.2 | 3.2067 | 0.2006 | 96.6% | 1263.2922 | 1263.3066 | 2 | 5.242 | 69.2% | 1 | R.SLLEGEGSSGGGGR.G | 2 |
| U | contaminant_KERATIN18 | 2 | 10 | 3.7% | 562 | 59822 | 8.0 | no description |
| U | contaminant_KERATIN22 | 2 | 10 | 3.3% | 645 | 65865 | 8.0 | no description |
| U | contaminant_KERATIN21 | 2 | 10 | 5.9% | 357 | 39219 | 5.2 | no description |
| Filename | XCorr | DeltCN | Conf% | ObsM+H+ | CalcM+H+ | SpR | ZScore | Ion% | # | Sequence | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mif2_01_itms.09075.09075.2 | 4.354 | 0.141 | 100.0% | 1332.1721 | 1330.5211 | 1 | 5.25 | 81.8% | 2 | R.NLDLDSIIAEVK.A | 2 | |
| Mif2_01_itms.21614.21614.3 | 6.0683 | 0.3998 | 100.0% | 2421.8342 | 2419.694 | 1 | 7.834 | 46.2% | 8 | R.NLDLDSIIAEVKAQYEEIAQR.S | 3 |
| Proteins | Peptide IDs | Spectra | |
| Unfiltered | 9654 | 40539 | 44076 |
| Filtered | 8 | 87 | 287 |
| Forward matches | 8 | 87 | 287 |
| Decoy matches | 0 | 0 | 0 |
| Forward FP rate | 0.0% | 0.0% | 0.0% |