DTASelect v2.0.37 /data/1/catclw/Projects/Jamie/Alpha_FT_4_01072010/splitted /garibaldi/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_06-30-2008_reversed.fasta SEQUEST 3.0 in SQT format. --trypstat --fp 0.1 -p 1 -e con true Use criteria 0.0 Minimum peptide confidence 0.1 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling con Exclude protein names matching 0 Minimum modified peptides per locus 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YIL149C 54 57 32.4% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * FT_4a_10.04644.04644.2 2.788 0.2258 100.0 1531.7711 1531.616 6618.7 5 4.602 54.2 1 K.EELNGLKDQLNEE.R * FT_4a_12.05442.05442.2 1.9136 0.0946 96.9 1455.8206 1453.5939 7039.0 1 4.037 60.0 1 R.VKEEYDIWQSR.D * FT_4a_20.08550.08550.2 1.5071 0.1435 92.6 1405.9607 1405.7131 5180.5 2 3.6 50.0 1 R.KLMEMENILQR.C * FT_4a_7.03752.03752.2 2.5889 0.3351 100.0 1172.5634 1172.2365 6099.7 1 6.362 77.8 1 K.SSHVENLEEK.L * FT_4a_11.05228.05228.2 2.6591 0.3297 100.0 1905.0134 1906.1455 6833.2 1 5.51 50.0 1 K.FLLNQNKQLSQSVEEK.V * FT_4a_7.03750.03750.2 1.58 0.1153 91.5 1104.5901 1105.2328 5415.0 1 4.377 55.6 1 K.NLKDTASVEK.A * FT_4a_18.07729.07729.3 4.0217 0.3892 100.0 2398.2397 2398.7334 7380.7 1 6.798 35.0 2 K.NLKDTASVEKAEFSKEMTLQK.N * FT_4a_11.05024.05024.1 1.9641 0.1791 92.9 875.4432 876.0179 2245.8 1 4.905 75.0 1 K.NMNDLLR.S * FT_4a_10.04904.04904.2 1.7852 0.283 100.0 1535.918 1537.6758 7245.2 3 4.798 45.8 1 R.SQLTSLEKDCSLR.A * FT_4a_20.08640.08640.3 4.25 0.4119 100.0 3043.4272 3043.1934 7272.9 1 6.664 28.0 1 R.AIEKNDDNSCRNPEHTDVIDELIDTK.L * FT_4a_15.06518.06518.2 3.2616 0.5167 100.0 1477.8348 1477.6978 6712.5 1 7.5 58.3 2 R.STELLETVSLTKR.K * FT_4a_18.07671.07671.2 4.7263 0.5931 100.0 2373.2297 2374.6099 9144.9 1 10.394 57.5 1 R.KILESSNIVNENDSQAIITER.L * FT_4a_19.08171.08171.2 3.9547 0.4525 100.0 2246.1216 2246.4358 7945.3 1 8.155 57.9 1 K.ILESSNIVNENDSQAIITER.L * FT_4a_17.07419.07419.2 3.8207 0.4391 100.0 1549.7959 1549.7203 6833.9 1 8.579 70.8 1 R.LVEFSNVNELQEK.N * FT_4a_22.09200.09200.1 2.7122 0.2266 100.0 1342.7257 1343.5194 6744.8 1 7.492 63.6 1 K.DAIIELENINAK.M * FT_4a_13.06036.06036.1 1.8188 0.1958 100.0 741.49945 741.9517 4033.4 5 4.648 70.0 1 R.INILLR.E * FT_4a_11.05309.05309.4 3.7405 0.2917 100.0 3116.5005 3117.371 5775.9 1 4.616 22.8 1 R.ERDSYKLLASTEENKANTNSVTSMEAAR.E * FT_4a_9.04530.04530.2 3.2141 0.4495 100.0 2337.1428 2338.5513 6225.8 1 7.193 38.1 1 K.LLASTEENKANTNSVTSMEAAR.E * FT_4a_12.05557.05557.2 2.0397 0.1307 98.4 1579.8376 1580.7777 4840.8 1 3.583 57.7 1 K.ETASFIPTKESLTR.D * FT_4a_8.04161.04161.2 1.7632 0.1162 97.0 1259.6648 1258.4166 5649.7 8 3.677 55.6 1 K.IKELENNLER.L * FT_4a_9.04431.04431.2 3.0458 0.2956 100.0 988.5511 988.12836 3132.3 1 5.667 92.9 1 K.IQEIESIR.S * FT_4a_10.04798.04798.1 1.9174 0.381 100.0 1451.76 1452.5781 6119.2 3 6.392 45.5 1 K.WAQNTIDDTEMK.M * FT_4a_10.04806.04806.2 2.9869 0.4029 100.0 1451.6489 1452.5781 5457.5 1 6.997 77.3 1 K.WAQNTIDDTEMK.M * FT_4a_7.03794.03794.2 2.0488 0.1697 100.0 814.4459 814.95917 3062.1 2 4.565 90.0 1 K.TKFQYK.F * FT_4a_20.08357.08357.2 4.0984 0.5695 100.0 1806.8907 1807.9543 7035.9 1 8.964 60.0 1 K.FLDQNSDASTLEPTLR.K * FT_4a_8.03904.03904.3 3.7362 0.3469 100.0 1710.9199 1709.9366 6838.6 1 7.37 46.2 1 K.TKENYDAKIELEKK.E * FT_4a_8.03882.03882.2 3.5139 0.5987 100.0 1591.7677 1592.7089 4592.8 1 9.187 76.9 1 R.LEKDAADCQAELTK.T * FT_4a_13.06028.06028.2 1.8362 0.2914 100.0 1470.8215 1471.7019 5954.3 102 4.581 36.4 1 K.LVSLFSNLRHER.N * FT_4a_10.04753.04753.1 1.755 0.2392 100.0 706.4158 706.8596 3816.1 1 6.26 80.0 1 R.ELAFVK.Q * FT_4a_8.03892.03892.1 1.6223 0.3098 100.0 859.4652 859.9573 4494.6 1 4.986 66.7 1 K.TINDLQR.T * FT_4a_18.07811.07811.2 2.5325 0.2826 100.0 1955.0928 1955.2615 8873.4 1 5.017 40.6 1 K.EVTQVNILKENNAILQK.S * FT_4a_7.03719.03719.2 1.9727 0.1574 100.0 1393.7485 1394.57 6266.0 1 5.379 55.0 1 K.NVTEKNREIYK.Q * FT_4a_20.08480.08480.3 4.7934 0.4061 100.0 2813.3682 2814.1055 8070.1 1 7.612 39.8 1 K.EQVSINSNKILVYESEMEQCKQR.Y * FT_4a_7.03762.03762.2 1.9157 0.3156 100.0 1137.5531 1138.2218 6400.8 1 4.558 68.8 1 R.YQDLSQQQK.D * FT_4a_9.04523.04523.4 4.0237 0.3429 100.0 3784.8125 3787.0886 7230.3 1 5.192 17.7 1 K.KDIEKLTNEISDLKGKLS*SAENANADLENKFNR.L * FT_4a_23.09513.09513.3 3.0209 0.166 100.0 3092.6619 3093.377 9648.3 14 4.561 19.4 1 K.LTNEISDLKGKLSSAENANADLENKFNR.L * FT_4a_9.04523.04523.2 4.3003 0.6106 100.0 1892.9098 1894.0074 7230.3 1 10.463 59.4 1 K.LSSAENANADLENKFNR.L * FT_4a_10.04697.04697.2 3.8506 0.4238 100.0 1586.7867 1587.7289 7229.2 1 7.031 83.3 1 K.DKLEQDLHFENAK.V * FT_4a_7.03784.03784.3 4.4469 0.3749 100.0 2557.251 2557.7385 8407.8 1 6.873 38.8 1 K.LKAHELQSEDVSRDHEKDTYR.T * FT_4a_7.03632.03632.2 3.0098 0.2475 100.0 1360.7909 1358.5803 7292.6 1 5.154 80.0 1 R.IKEAEENLKKR.I * FT_4a_7.03826.03826.2 2.2679 0.3558 100.0 1098.6345 1098.2871 3392.0 1 6.211 75.0 1 K.NLKPQEQLK.N * FT_4a_12.05585.05585.1 1.581 0.3001 100.0 1211.7026 1212.4314 7088.1 1 5.041 50.0 1 R.TLNIQPEVAVK.A * FT_4a_12.05574.05574.2 2.4602 0.3661 100.0 1211.7003 1212.4314 6719.8 3 6.77 60.0 1 R.TLNIQPEVAVK.A * FT_4a_22.09216.09216.2 3.3324 0.3967 100.0 2053.9778 2054.1772 7884.7 1 7.188 47.4 2 K.AAINFSNVTDLTNNSTDGAK.I * FT_4a_24.10125.10125.3 3.3432 0.2693 100.0 2970.4856 2971.204 8114.8 2 4.868 24.1 1 K.AAINFSNVTDLTNNSTDGAKITEIGSTSK.R * FT_4a_22.09162.09162.4 3.0499 0.2157 100.0 4440.168 4442.748 8525.6 1 3.643 18.3 1 K.AAINFSNVTDLTNNSTDGAKITEIGSTSKRPIESGTSSDPDTK.K * FT_4a_21.08791.08791.4 3.22 0.1746 100.0 4571.3003 4570.922 8297.1 2 3.384 14.7 1 K.AAINFSNVTDLTNNSTDGAKITEIGSTSKRPIESGTSSDPDTKK.V * FT_4a_21.08719.08719.2 5.6657 0.6105 100.0 1911.9191 1912.0197 8061.4 1 10.273 64.7 1 A.INFSNVTDLTNNSTDGAK.I * FT_4a_7.03837.03837.1 1.8517 0.4658 100.0 935.5057 936.04956 4243.0 1 7.55 62.5 1 K.ITEIGSTSK.R * FT_4a_7.03828.03828.3 3.2169 0.2542 100.0 2535.3013 2535.7673 4681.3 1 4.676 35.9 1 K.ITEIGSTSKRPIESGTSSDPDTKK.V * FT_4a_7.03706.03706.2 2.9258 0.4012 100.0 2320.1675 2321.503 5027.8 1 5.737 35.7 1 T.EIGSTSKRPIESGTSSDPDTKK.V * FT_4a_7.03662.03662.2 2.8785 0.3707 100.0 1490.718 1490.5664 3433.0 1 5.996 76.9 1 K.RPIESGTSSDPDTK.K * FT_4a_7.03606.03606.2 2.7842 0.2602 100.0 1618.8145 1618.7405 3977.9 1 5.953 60.7 1 K.RPIESGTSSDPDTKK.V * FT_4a_7.03629.03629.2 2.7523 0.3082 100.0 1844.9757 1846.0471 4302.7 3 6.855 43.8 1 K.RPIESGTSSDPDTKKVK.E YIL002W-A 1 1 30.4% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * FT_4a_9.04420.04420.3 4.1282 0.4249 100.0 2410.2368 2411.6335 7823.1 1 6.67 38.8 1 L.LVQLEDLHRDNNDLAKSSSQK.- YLR150W 4 4 28.2% 273 29995 9.7 U STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations" * FT_4a_21.08753.08753.4 2.8713 0.3497 100.0 5023.565 5025.38 8185.1 111 5.38 14.0 1 K.KSNTRRATDRHSRTGKTDTKKKVNQGWGDDKKELS*AEKEAQADA.A * FT_4a_9.04360.04360.2 1.3552 0.1442 90.3 1076.9148 1079.0769 2606.5 36 3.773 64.3 1 R.RAT*DRHSR.T * FT_4a_25.10204.10204.3 3.2378 0.2812 100.0 2863.4526 2864.1436 8155.5 1 4.873 25.0 1 K.TAQLSLQDYLNQQANNQFNKVPEAK.K * FT_4a_47.18437.18437.1 1.0783 0.1969 94.1 958.6853 959.0001 5214.8 183 3.686 35.7 1 K.EAYVPAT*K.V YLR441C 3 3 17.3% 255 28743 10.0 U RPS1A SGDID:S000004433, Chr XII from 1018904-1018137, reverse complement, Verified ORF, "Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarity to rat S3a ribosomal protein" YML063W 3 3 17.3% 255 28812 10.0 U RPS1B SGDID:S000004528, Chr XIII from 146482-147249, Verified ORF, "Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarity to rat S3a ribosomal protein" FT_4a_21.08966.08966.2 1.7289 0.1103 92.8 1867.9684 1869.0862 8008.6 15 3.33 32.1 1 R.KEWFDIKAPSTFENR.N FT_4a_22.09086.09086.2 4.7605 0.5968 100.0 2061.9805 2062.2092 8454.8 1 10.758 67.6 1 R.VVEVCLADLQGSEDHSFR.K FT_4a_24.09850.09850.2 1.8737 0.2692 100.0 1351.7528 1352.5785 5837.1 1 6.308 60.0 1 K.DIFPLQNIHVR.K YDL229W 6 6 13.9% 613 66602 5.4 U SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p" * FT_4a_22.09245.09245.2 1.8082 0.2213 100.0 1479.9254 1480.6599 8813.2 1 5.391 54.2 1 R.VTPSFVAFTPEER.L FT_4a_24.09960.09960.1 2.2491 0.3197 100.0 1167.6095 1168.3342 8951.1 1 5.966 72.2 1 R.FEDLNAALFK.S FT_4a_21.08886.08886.2 2.5796 0.3252 100.0 1556.8563 1557.7838 5649.0 1 7.057 57.7 1 K.STLEPVEQVLKDAK.I * FT_4a_21.08986.08986.2 4.7016 0.5144 100.0 2464.1301 2464.616 6747.7 1 8.884 45.0 1 R.TFTTCADNQTTVQFPVYQGER.V FT_4a_24.09933.09933.2 4.1763 0.4251 100.0 1779.8964 1780.9813 7240.3 1 8.401 75.0 1 R.VNCKENTLLGEFDLK.N FT_4a_9.04456.04456.1 1.9575 0.3733 100.0 1218.6475 1219.3397 4246.8 1 6.49 63.6 1 K.SSNITISNAVGR.L YIL148W 1 1 12.5% 128 14554 9.8 U RPL40A SGDID:S000001410, Chr IX from 68708-68715,69150-69528, Verified ORF, "Fusion protein, identical to Rpl40Bp, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes" YLR167W 1 1 10.5% 152 17216 9.9 U RPS31 SGDID:S000004157, Chr XII from 498949-499407, Verified ORF, "Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B" YLL039C 1 1 4.2% 381 42826 7.6 U UBI4 SGDID:S000003962, Chr XII from 65206-64061, reverse complement, Verified ORF, "Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response" YKR094C 1 1 12.5% 128 14554 9.8 U RPL40B SGDID:S000001802, Chr XI from 618392-618385,618016-617638, reverse complement, Verified ORF, "Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of the large (60S) ribosomal subunit with similarity to rat L40; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes" FT_4a_20.08464.08464.2 3.788 0.5213 100.0 1763.8989 1764.9238 7850.7 1 9.13 63.3 1 K.TITLEVESSDTIDNVK.S YNL209W 5 5 11.7% 613 66595 5.5 U SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; homolog of SSB1" FT_4a_24.09960.09960.1 2.2491 0.3197 100.0 1167.6095 1168.3342 8951.1 1 5.966 72.2 1 R.FEDLNAALFK.S FT_4a_21.08886.08886.2 2.5796 0.3252 100.0 1556.8563 1557.7838 5649.0 1 7.057 57.7 1 K.STLEPVEQVLKDAK.I * FT_4a_22.09407.09407.2 2.3457 0.2448 100.0 2418.165 2419.6091 7524.3 1 4.891 32.5 1 R.TFTTVSDNQTTVQFPVYQGER.V FT_4a_24.09933.09933.2 4.1763 0.4251 100.0 1779.8964 1780.9813 7240.3 1 8.401 75.0 1 R.VNCKENTLLGEFDLK.N FT_4a_9.04456.04456.1 1.9575 0.3733 100.0 1218.6475 1219.3397 4246.8 1 6.49 63.6 1 K.SSNITISNAVGR.L YLR044C 3 3 11.0% 563 61495 6.2 U PDC1 SGDID:S000004034, Chr XII from 234082-232391, reverse complement, Verified ORF, "Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism" * FT_4a_19.08266.08266.2 4.9465 0.5483 100.0 1998.9149 1999.1057 9276.1 1 9.878 61.1 1 R.WAGNANELNAAYAADGYAR.I * FT_4a_22.09056.09056.3 2.475 0.1706 100.0 2108.0923 2106.394 6158.5 8 3.439 30.9 1 K.QLLLHHTLGNGDFTVFHR.M * FT_4a_33.13378.13378.2 2.0274 0.3411 100.0 2743.715 2744.206 7035.3 1 5.584 29.2 1 R.TTYVTQRPVYLGLPANLVDLNVPAK.L YPL255W 2 2 10.6% 385 45384 6.5 U BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p" * FT_4a_21.08710.08710.4 3.0658 0.1584 100.0 3118.815 3123.1484 4418.1 345 3.977 22.9 1 K.Y*ELLPEYNENGFNS*IVNGDHHSKER.I * FT_4a_24.09826.09826.2 3.1992 0.2138 100.0 2014.0547 2012.2665 9014.3 1 5.649 53.3 1 R.LYQEEDLKNFEIQTLK.Q YMR142C 1 1 10.6% 199 22525 11.1 U RPL13B SGDID:S000004750, Chr XIII from 551206-551203,550800-550205, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential for viability; has similarity to rat L13 ribosomal protein" * FT_4a_23.09416.09416.3 3.3133 0.2564 100.0 2096.3813 2095.4087 7407.8 1 5.42 32.5 1 R.AGRGFTLAEVKAAGLTAAYAR.T YHR174W 3 4 9.4% 437 46914 6.0 U ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" FT_4a_21.08946.08946.3 6.0554 0.4373 100.0 2585.3164 2584.845 8777.1 1 7.747 43.2 1 R.SVYDSRGNPTVEVELTTEKGVFR.S FT_4a_21.08681.08681.2 3.6743 0.4638 100.0 1878.9945 1877.104 7850.7 1 7.32 59.4 1 R.GNPTVEVELTTEKGVFR.S * FT_4a_25.10412.10412.2 5.0751 0.5021 100.0 1858.0001 1856.0856 9584.8 1 8.376 73.5 2 K.TAGIQIVADDLTVTNPAR.I YDR356W 6 6 9.0% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * FT_4a_7.03815.03815.3 2.9424 0.151 100.0 2118.942 2119.236 6042.3 1 5.406 35.9 1 R.KVDDHSGCIEEREQMER.K * FT_4a_21.08697.08697.3 3.282 0.2069 100.0 3239.689 3241.7092 9651.0 2 4.08 19.4 1 K.LAELERKLKTVKDQVLELENNSDVQSLK.L * FT_4a_21.08697.08697.2 5.1655 0.5321 100.0 2160.1284 2160.3855 9651.0 1 9.595 63.9 1 K.TVKDQVLELENNSDVQSLK.L * FT_4a_10.04734.04734.3 2.0574 0.1956 96.0 1777.9755 1779.0428 4523.8 84 3.712 32.1 1 K.ILENDLKVAQEKYSK.M * FT_4a_9.04248.04248.1 1.704 0.2635 100.0 1287.6935 1288.443 5103.3 1 5.521 54.5 1 K.ISNLAAENSQLK.N * FT_4a_7.03807.03807.2 3.5838 0.4346 100.0 1588.7615 1589.6586 4615.2 1 7.841 79.2 1 R.EKEELNENSNNIR.I YDR385W 4 4 8.0% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" YOR133W 4 4 8.0% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" FT_4a_31.12646.12646.2 1.6427 0.3422 100.0 2124.3264 2125.429 7695.0 2 5.247 29.4 1 R.ALLELQVSKEDLYQTFAR.T FT_4a_19.08154.08154.3 2.5383 0.184 100.0 2478.283 2479.746 4145.4 17 3.707 28.4 1 K.ISPPVVAYRETVESESSQTALSK.S FT_4a_21.09020.09020.2 2.9292 0.4184 100.0 1627.9982 1628.7529 5970.1 1 6.493 61.5 1 R.IMADDYGWDVTDAR.K FT_4a_24.09840.09840.2 3.0814 0.3387 100.0 1268.683 1267.4668 6824.5 1 6.786 68.2 1 R.ATYAGFLLADPK.I YAL005C 3 3 4.7% 642 69768 5.1 U SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall" FT_4a_22.09383.09383.2 2.9901 0.3787 100.0 1199.6766 1200.38 5580.0 1 7.408 86.4 1 K.DAGTIAGLNVLR.I * FT_4a_14.06405.06405.2 1.4797 0.1187 90.4 1275.7854 1275.4924 5294.5 143 3.553 44.4 1 R.LVNHFIQEFK.R * FT_4a_7.03771.03771.2 2.2092 0.2162 100.0 961.5208 962.08746 5252.3 2 5.256 78.6 1 R.LSKEDIEK.M YMR186W 2 2 4.1% 705 80900 4.8 U HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels that HSP82 and induced 2-3 fold by heat shock" * FT_4a_20.08452.08452.2 4.2884 0.4856 100.0 1818.8224 1819.9214 8137.1 1 8.323 75.0 1 R.NPSDITQEEYNAFYK.S * FT_4a_28.11587.11587.2 2.1991 0.2912 100.0 1600.1431 1599.7344 7527.8 4 4.992 50.0 1 K.SVDELTSLTDYVTR.M YGL008C 3 3 4.0% 918 99619 5.1 U PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases" * FT_4a_18.07680.07680.3 5.9324 0.5013 100.0 3022.4358 3022.211 6929.8 1 8.349 38.0 1 K.TVEEDHPIPEDVHENYENKVAELASR.G * FT_4a_18.07682.07682.4 4.3028 0.4668 100.0 3024.4482 3022.211 7861.3 1 7.431 26.7 1 K.TVEEDHPIPEDVHENYENKVAELASR.G * FT_4a_25.10184.10184.2 2.2269 0.3171 100.0 1284.5759 1285.475 3806.4 1 5.075 70.0 1 R.SVEDFMAAMQR.V Reverse_YGR254W 1 1 3.4% 437 46816 6.6 U ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose" Reverse_YHR174W 1 1 3.4% 437 46914 6.0 U ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" FT_4a_11.05212.05212.2 1.6333 0.1615 95.5 1673.6837 1674.8134 5873.7 129 3.361 32.1 1 K.GDKFFESSACDLGIK.I Reverse_YNL225C 1 1 2.9% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * FT_4a_27.10897.10897.2 1.7114 0.1346 93.9 2029.3892 2031.149 6936.5 28 3.449 28.1 1 K.IT*IRESTNGELNGFRSR.L YNL188W 1 1 2.8% 433 50653 9.3 U KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p" * FT_4a_17.07279.07279.2 2.1731 0.1065 98.4 1545.9508 1545.6428 8238.3 5 3.664 54.5 1 R.KEEVFT*DEVLQK.K YDR172W 1 1 2.5% 685 76551 7.0 U SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a dominant cytoplasmically inherited protein aggregate that alters translational fidelity and creates a nonsense suppressor phenotype" * FT_4a_23.09656.09656.2 3.5406 0.4248 100.0 1813.9375 1813.0784 9129.5 1 6.324 53.1 1 K.STMGGNLLYLTGSVDKR.T Reverse_YGL173C 2 2 2.3% 1528 175459 7.5 U KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonuclease involved in mRNA decay, evolutionarily conserved component of cytoplasmic processing (P) bodies, plays a role in microtubule-mediated processes, filamentous growth, and ribosomal RNA maturation" * FT_4a_22.09309.09309.2 2.2717 0.1168 98.4 2016.0653 2016.2675 8108.7 1 3.447 46.9 1 K.TNNNRNNSQKKKELAKK.S * FT_4a_23.09695.09695.2 1.8365 0.1075 92.5 2054.044 2055.2458 7460.0 15 3.776 29.4 1 K.T*LSDSELSVAIFKSSVYK.L Reverse_YNL188W 1 1 1.8% 433 50653 9.3 U KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p" * FT_4a_12.05469.05469.2 1.5693 0.1478 96.9 1018.49274 1017.93536 4292.6 208 3.422 57.1 1 K.EKSDT*DDK.L Reverse_YJR053W 1 1 1.7% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * FT_4a_21.08717.08717.2 1.5054 0.1356 91.3 1163.7886 1165.3372 4437.7 1 4.103 50.0 1 K.SRLFPSSSRK.K Reverse_YKR095W 1 1 1.3% 1875 218455 5.2 U MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs" * FT_4a_15.06447.06447.3 2.2348 0.1731 92.0 2816.5154 2818.1938 6889.5 193 3.226 18.8 1 K.ESKLTIIAEKAENVTESEIKQLSQK.S YHR172W 2 2 1.3% 823 96825 6.4 U SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * FT_4a_41.16067.16067.1 1.1707 0.1795 94.7 715.5051 715.87354 4919.7 9 4.055 60.0 1 R.IVRATR.V * FT_4a_50.19580.19580.1 1.12 0.182 95.2 894.64386 896.08 5318.6 1 3.953 57.1 1 R.ATRVLHAK.M Reverse_YHR172W 1 1 1.2% 823 96825 6.4 U SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * FT_4a_31.12576.12576.1 1.7688 0.1199 90.5 1284.25 1283.3147 6926.7 14 3.313 44.4 1 R.MLNNPNES*QR.E YMR129W 1 1 1.1% 1337 151652 6.7 U POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase;" * FT_4a_9.04247.04247.2 2.4711 0.3363 100.0 1691.8118 1691.8839 5782.9 3 5.495 46.4 1 S.KERSSQFVRLASEPG.I Reverse_YLR342W 1 1 0.8% 1876 214849 7.2 U FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling" * FT_4a_8.04047.04047.4 2.1363 0.1907 95.8 1793.8676 1797.0885 4735.7 443 3.829 29.8 1 K.AKRAKRS*LKGLSMNR.F YLR342W 1 1 0.4% 1876 214849 7.2 U FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling" * FT_4a_47.18662.18662.1 1.2832 0.2488 100.0 793.4348 792.95605 4384.8 6 5.107 58.3 1 R.ARITGFK.R Proteins Peptide IDs Spectra Unfiltered 136 14821 17227 Filtered 29 112 116 Forward matches 21 103 107 Decoy matches 8 9 9 Forward FP rate 38.1 8.74 8.41 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0