DTASelect v2.0.26 /data/1/catclw/Projects/Jamie/Jamie_June2008/Alpha_SPB_red/splitted/phospho_2 /scratch/yates/SGD_S-cerevisiae_na_07-09-2008_reversed.fasta SEQUEST 3.0 in SQT format. --trypstat --cmax 4 --fp 0.2 -p 1 true Use criteria 0.0 Minimum peptide confidence 0.2 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YIL002W-A 14 15 98.6% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * Jamie_Alpha_SPB_13.04464.04464.2 4.2535 0.3848 100.0 1447.5521 1448.5297 6514.1 1 7.528 76.9 1 M.TRDTPEDVSTAGAK.D * Jamie_Alpha_SPB_41.14755.14755.2 3.1587 0.3279 100.0 2117.372 2117.3176 7346.6 1 5.775 52.6 1 M.TRDTPEDVSTAGAKDILDVL.N * Jamie_Alpha_SPB_44.15644.15644.2 4.0437 0.3587 100.0 2197.4321 2197.3176 6346.3 1 7.578 55.3 1 M.TRDT*PEDVSTAGAKDILDVL.N * Jamie_Alpha_SPB_59.21142.21142.3 6.0139 0.4593 100.0 2584.4944 2585.9146 9218.5 1 8.07 50.0 1 M.TRDTPEDVSTAGAKDILDVLNLLK.G * Jamie_Alpha_SPB_30.10854.10854.3 3.0616 0.1708 98.4 2062.7043 2064.3125 8908.3 1 3.93 36.8 1 K.GGEEKISEVELKLDEMEK.K * Jamie_Alpha_SPB_31.10897.10897.2 5.644 0.4281 100.0 2063.7322 2064.3125 8727.9 1 9.702 73.5 2 K.GGEEKISEVELKLDEMEK.K * Jamie_Alpha_SPB_32.11360.11360.2 3.4046 0.3386 100.0 2192.5322 2192.4866 7341.3 1 6.899 47.2 1 K.GGEEKISEVELKLDEMEKK.M * Jamie_Alpha_SPB_31.11064.11064.3 4.5702 0.216 100.0 2192.5444 2192.4866 7007.3 1 6.338 38.9 1 K.GGEEKISEVELKLDEMEKK.M * Jamie_Alpha_SPB_26.09086.09086.2 2.4202 0.2235 99.4 1563.0322 1563.8036 6283.3 1 4.55 62.5 1 K.ISEVELKLDEMEK.K * Jamie_Alpha_SPB_37.13146.13146.3 2.7596 0.1494 93.8 1698.0543 1697.9904 4962.9 392 5.068 36.5 1 K.KMDSLLVQLEDLHR.D * Jamie_Alpha_SPB_37.13190.13190.2 2.8238 0.1016 99.0 1699.7722 1697.9904 6841.3 2 3.72 50.0 1 K.KMDSLLVQLEDLHR.D * Jamie_Alpha_SPB_37.13372.13372.3 3.3476 0.3246 100.0 2425.7344 2427.6917 8310.8 7 5.506 28.8 1 K.MDSLLVQLEDLHRDNNDLAKS.S * Jamie_Alpha_SPB_20.07207.07207.3 3.0036 0.254 100.0 1894.1344 1894.0946 5060.4 3 4.81 43.3 1 L.LVQLEDLHRDNNDLAK.S * Jamie_Alpha_SPB_14.05037.05037.2 1.902 0.2689 81.6 1957.4922 1958.0519 4515.7 4 4.151 37.5 1 L.EDLHRDNNDLAKSSSQK.- YIL149C 144 177 57.6% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_Alpha_SPB_37.13123.13123.3 6.5838 0.3824 100.0 3503.3044 3501.8296 9579.2 1 6.289 32.8 1 K.LKQLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Alpha_SPB_37.13194.13194.4 5.1427 0.3504 100.0 3503.4165 3501.8296 6506.6 1 5.78 24.1 3 K.LKQLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Alpha_SPB_38.13638.13638.3 3.4729 0.2508 100.0 3744.6243 3745.0952 10379.0 48 5.553 18.5 2 K.LKQLLDESSEQKNTAKEELNGLKDQLNEERSR.Y * Jamie_Alpha_SPB_38.13402.13402.4 3.174 0.1912 89.7 3746.9365 3745.0952 9700.7 1 4.175 20.4 1 K.LKQLLDESSEQKNTAKEELNGLKDQLNEERSR.Y * Jamie_Alpha_SPB_33.11850.11850.4 4.3804 0.2629 100.0 3262.2966 3260.496 6081.7 1 4.823 25.9 1 K.QLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Alpha_SPB_22.07740.07740.2 2.6435 0.3988 100.0 1687.6322 1687.804 5318.0 1 6.37 69.2 1 K.EELNGLKDQLNEER.S * Jamie_Alpha_SPB_27.09556.09556.3 3.9395 0.1884 98.2 3253.8542 3254.4075 8997.2 12 4.426 24.0 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Alpha_SPB_27.09657.09657.4 6.0683 0.3782 100.0 3256.7766 3254.4075 4320.0 1 5.941 28.8 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Alpha_SPB_36.12951.12951.3 3.9623 0.2857 100.0 3636.9243 3636.9138 7006.8 1 5.722 24.1 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENKLLR.R * Jamie_Alpha_SPB_23.08217.08217.2 4.0147 0.3301 100.0 1820.4922 1819.8363 7120.3 1 6.183 60.0 3 R.DQGNDSLNDDLNKENK.L * Jamie_Alpha_SPB_26.09138.09138.3 3.8136 0.3071 100.0 2358.7144 2358.5303 5536.6 2 5.16 31.6 1 R.DQGNDSLNDDLNKENKLLRR.K * Jamie_Alpha_SPB_32.11332.11332.2 2.2606 0.2806 100.0 2016.1522 2016.2324 9398.5 13 4.916 40.0 1 K.LYQMQSNYESVFTYNK.F * Jamie_Alpha_SPB_18.06235.06235.2 2.8025 0.313 100.0 1668.2722 1667.8564 6650.2 1 5.127 53.6 1 K.NLKDTASVEKAEFSK.E * Jamie_Alpha_SPB_36.12882.12882.4 6.9505 0.4131 100.0 3311.5366 3312.5408 8587.2 1 6.875 32.1 1 R.AIEKNDDNSCRNPEHTDVIDELIDTKLR.L * Jamie_Alpha_SPB_36.12933.12933.3 4.8495 0.3749 100.0 3313.3442 3312.5408 9869.0 1 6.226 31.5 1 R.AIEKNDDNSCRNPEHTDVIDELIDTKLR.L * Jamie_Alpha_SPB_40.14340.14340.4 5.3517 0.3441 100.0 3680.2566 3682.9897 9851.1 1 6.52 24.4 1 R.AIEKNDDNSCRNPEHTDVIDELIDTKLRLEK.S * Jamie_Alpha_SPB_40.14310.14310.3 3.5729 0.0668 82.5 3684.8044 3682.9897 8760.1 1 4.053 25.0 1 R.AIEKNDDNSCRNPEHTDVIDELIDTKLRLEK.S * Jamie_Alpha_SPB_32.11302.11302.3 3.326 0.2631 100.0 2600.6343 2601.666 7920.2 24 4.384 26.2 2 K.NDDNSCRNPEHTDVIDELIDTK.L * Jamie_Alpha_SPB_31.11136.11136.2 3.7136 0.3163 100.0 2601.392 2601.666 4606.7 1 6.0 45.2 1 K.NDDNSCRNPEHTDVIDELIDTK.L * Jamie_Alpha_SPB_33.11682.11682.3 4.652 0.2464 100.0 1740.3844 1739.8767 8883.6 1 5.215 50.0 1 R.NPEHTDVIDELIDTK.L * Jamie_Alpha_SPB_4.01306.01306.1 1.647 0.3164 100.0 759.3 758.8931 6619.1 1 5.539 71.4 1 S.AVSPTVGK.L * Jamie_Alpha_SPB_66.23682.23682.4 4.4868 0.2156 91.3 3717.3364 3717.2163 8063.4 1 5.27 22.4 1 R.NQKFQLQNQLEDFILELEHKTPELISFKER.T * Jamie_Alpha_SPB_60.21495.21495.2 2.1692 0.1924 99.5 2145.652 2145.4197 8096.2 5 4.585 37.5 1 K.FQLQNQLEDFILELEHK.T * Jamie_Alpha_SPB_20.07170.07170.2 3.3554 0.3068 100.0 1240.7922 1241.4331 7227.8 1 6.024 88.9 1 R.TKSLEHELKR.S * Jamie_Alpha_SPB_45.16029.16029.2 3.8612 0.3269 100.0 2581.1921 2580.8088 7695.1 1 6.948 36.4 1 L.LLNTSAIQETAS*PLSQDELISLR.K * Jamie_Alpha_SPB_30.10760.10760.2 3.1382 0.3921 100.0 2267.2122 2267.455 3684.7 1 6.598 44.7 1 T.SAIQETAS*PLSQDELISLRK.I * Jamie_Alpha_SPB_25.08868.08868.3 4.3079 0.3315 100.0 2374.6443 2374.6104 7673.3 1 5.754 41.2 1 R.KILESSNIVNENDSQAIITER.L * Jamie_Alpha_SPB_26.09093.09093.2 3.1842 0.2339 100.0 2374.672 2374.6104 8436.5 1 5.144 50.0 1 R.KILESSNIVNENDSQAIITER.L * Jamie_Alpha_SPB_28.10112.10112.2 2.8796 0.0115 93.2 2248.8123 2246.4363 8210.1 1 2.956 44.7 1 K.ILESSNIVNENDSQAIITER.L * Jamie_Alpha_SPB_30.10531.10531.2 2.9597 0.2786 100.0 2325.5322 2326.4363 7398.6 45 5.369 34.2 1 K.ILESSNIVNENDS*QAIITER.L * Jamie_Alpha_SPB_20.07035.07035.2 3.0161 0.2072 90.3 1805.8922 1803.9237 8135.4 2 4.536 50.0 1 S.SNIVNENDSQAIITER.L * Jamie_Alpha_SPB_24.08586.08586.2 3.1383 0.3266 100.0 1548.0322 1549.721 8030.6 1 6.496 75.0 2 R.LVEFSNVNELQEK.N * Jamie_Alpha_SPB_42.14880.14880.2 2.4218 0.0936 97.9 2661.2922 2661.9812 6667.8 1 4.144 42.9 1 R.LVEFSNVNELQEKNVELLNCIR.I * Jamie_Alpha_SPB_19.06658.06658.2 2.7946 0.1911 99.4 1765.1322 1764.9731 7111.5 1 4.307 53.6 1 R.ILADKLENYEGKQDK.T * Jamie_Alpha_SPB_45.15961.15961.2 2.6336 0.2585 91.7 2384.4321 2385.677 6776.5 1 5.477 45.0 1 V.ENQTIKEAKDAIIELENINAK.M * Jamie_Alpha_SPB_30.10668.10668.1 2.4189 0.1959 100.0 1342.37 1343.5199 6758.4 4 5.553 59.1 1 K.DAIIELENINAK.M * Jamie_Alpha_SPB_30.10638.10638.2 4.9988 0.276 100.0 1344.3922 1343.5199 8512.9 1 6.638 81.8 1 K.DAIIELENINAK.M * Jamie_Alpha_SPB_20.07114.07114.2 3.2016 0.4503 100.0 2083.7922 2085.2322 5690.8 1 7.781 47.2 1 R.DSYKLLASTEENKANTNSV.T * Jamie_Alpha_SPB_25.08728.08728.3 3.663 0.2489 100.0 2832.7144 2832.0686 10149.9 1 4.917 31.0 1 R.DSYKLLASTEENKANTNSVTSMEAAR.E * Jamie_Alpha_SPB_8.02747.02747.2 2.0155 0.2594 100.0 1003.6722 1005.11304 3712.2 1 5.129 87.5 1 K.LLASTEENK.A * Jamie_Alpha_SPB_8.02765.02765.1 1.7909 0.0684 95.2 1003.96 1005.11304 6377.5 9 3.085 62.5 1 K.LLASTEENK.A * Jamie_Alpha_SPB_22.07944.07944.1 1.5226 0.2937 100.0 1187.42 1188.3666 6317.9 16 4.924 44.4 1 K.KIRELEAELS.S * Jamie_Alpha_SPB_34.12255.12255.4 4.5023 0.2717 100.0 2742.1765 2743.133 9138.8 1 4.693 31.9 1 K.IRELEAELSSTKVENSAIIQNLRK.E * Jamie_Alpha_SPB_33.11599.11599.2 2.5096 0.1076 98.7 2345.5723 2345.612 6531.2 1 5.025 37.5 1 R.ELEAELSSTKVENSAIIQNLR.K * Jamie_Alpha_SPB_19.06817.06817.2 2.4318 0.2164 82.1 1701.0122 1701.9639 6862.0 1 4.362 57.1 1 S.STKVENSAIIQNLRK.E * Jamie_Alpha_SPB_30.10834.10834.2 2.871 0.3374 100.0 1242.3722 1244.3434 6422.2 1 5.977 83.3 1 K.TTLEDFENFK.G * Jamie_Alpha_SPB_33.11725.11725.2 2.8096 0.2839 100.0 1612.9922 1613.8076 7139.7 1 5.514 57.7 1 K.TTLEDFENFKGLAK.E * Jamie_Alpha_SPB_19.06582.06582.2 3.2752 0.3074 100.0 1339.6721 1339.5345 5186.8 1 6.052 75.0 1 I.DHLKAELEKQK.S * Jamie_Alpha_SPB_21.07612.07612.2 2.0388 0.0172 80.4 2123.6921 2124.33 5663.0 39 3.672 35.3 1 R.LKESEISHNENKMDFSSK.E * Jamie_Alpha_SPB_22.07884.07884.2 2.8823 0.2449 100.0 1457.6322 1457.67 7954.7 11 5.894 54.5 1 K.AKIKELENNLER.L * Jamie_Alpha_SPB_15.05354.05354.1 2.3273 0.1953 100.0 988.41 988.1289 4885.5 1 3.915 78.6 2 K.IQEIESIR.S * Jamie_Alpha_SPB_29.10304.10304.2 1.6888 0.1077 91.8 2024.4321 2024.2097 7656.4 24 3.585 37.5 1 K.DSQLKWAQNTIDDTEMK.M * Jamie_Alpha_SPB_33.11805.11805.2 2.8244 0.3776 100.0 2282.3123 2283.5764 7287.6 5 6.165 33.3 1 K.DSQLKWAQNTIDDTEMKMK.S * Jamie_Alpha_SPB_24.08623.08623.2 2.4459 0.0503 97.6 1007.0122 1005.15643 4644.5 7 3.844 81.2 1 K.SLLTELSNK.E * Jamie_Alpha_SPB_69.24691.24691.4 6.6569 0.4298 100.0 3234.4966 3234.6257 8704.7 1 6.62 35.8 3 K.SLLTELSNKETTIEKLSSEIENLDKELR.K * Jamie_Alpha_SPB_69.24558.24558.3 3.0528 0.1538 94.0 3237.4143 3234.6257 8726.3 22 3.921 22.2 1 K.SLLTELSNKETTIEKLSSEIENLDKELR.K * Jamie_Alpha_SPB_68.24405.24405.4 4.8545 0.2427 100.0 3364.6565 3362.7998 9077.9 1 4.942 26.2 1 K.SLLTELSNKETTIEKLSSEIENLDKELRK.T * Jamie_Alpha_SPB_17.05982.05982.2 3.1934 0.3133 100.0 1147.8922 1148.2554 6218.4 1 5.447 88.9 1 K.LSSEIENLDK.E * Jamie_Alpha_SPB_23.08191.08191.2 3.1921 0.1331 80.5 1434.5922 1433.5583 7478.0 1 4.334 81.8 1 L.SSEIENLDKELR.K * Jamie_Alpha_SPB_24.08545.08545.2 3.6091 0.1712 89.3 1562.5721 1561.7324 5675.9 1 4.343 75.0 1 L.SSEIENLDKELRK.T * Jamie_Alpha_SPB_30.10729.10729.3 2.4522 0.1105 80.6 2502.4443 2502.7864 9375.3 3 3.152 27.5 1 K.FQYKFLDQNSDASTLEPTLRK.E * Jamie_Alpha_SPB_29.10363.10363.2 2.6969 0.1099 98.6 1809.1122 1807.9548 7958.3 1 3.561 53.3 1 K.FLDQNSDASTLEPTLR.K * Jamie_Alpha_SPB_60.21589.21589.2 3.8896 0.2596 100.0 2695.7722 2693.9243 6814.1 1 4.789 41.3 1 K.DANSQIQAYEEIISSNENALIELK.N * Jamie_Alpha_SPB_42.15032.15032.2 3.2683 0.2687 100.0 2257.2322 2258.531 8149.1 2 6.058 42.1 1 Y.EEIISSNENALIELKNELAK.T * Jamie_Alpha_SPB_18.06261.06261.2 2.9244 0.314 100.0 1580.0322 1581.7631 6676.6 1 5.956 62.5 1 K.TKENYDAKIELEK.K * Jamie_Alpha_SPB_27.09496.09496.2 2.0055 0.0216 80.6 1967.7522 1967.2267 7723.4 21 3.663 36.7 1 K.TKENYDAKIELEKKEK.W * Jamie_Alpha_SPB_31.11004.11004.3 2.859 0.219 98.1 2160.2944 2160.435 7695.3 2 5.137 40.6 1 K.IELEKKEKWAREEDLSR.L * Jamie_Alpha_SPB_18.06457.06457.2 2.2761 0.0839 97.6 1419.0721 1419.537 4184.7 270 3.587 55.0 1 K.EKWAREEDLSR.L * Jamie_Alpha_SPB_18.06450.06450.3 2.0809 0.2112 98.4 1420.2244 1419.537 3659.1 3 4.19 42.5 1 K.EKWAREEDLSR.L * Jamie_Alpha_SPB_22.07770.07770.2 4.0576 0.3749 100.0 1613.5122 1614.8418 7589.3 1 6.76 69.2 2 K.LKEGALHFVQQSEK.L * Jamie_Alpha_SPB_11.03951.03951.2 2.7358 0.1797 100.0 917.2322 916.02496 5081.5 7 3.993 91.7 1 K.LRNEVER.I * Jamie_Alpha_SPB_30.10575.10575.1 2.1966 0.2748 100.0 945.34 945.1039 4752.7 6 4.489 71.4 1 K.DLSELVIR.L * Jamie_Alpha_SPB_30.10536.10536.2 2.5851 0.0219 96.9 946.83215 945.1039 5421.6 1 3.752 92.9 1 K.DLSELVIR.L * Jamie_Alpha_SPB_38.13681.13681.2 2.2318 0.0645 95.9 1314.2122 1315.5529 5132.6 90 3.974 60.0 1 K.DLSELVIRLEK.D * Jamie_Alpha_SPB_56.19915.19915.3 3.7155 0.3676 100.0 2517.1743 2518.79 8485.6 1 5.602 28.6 1 K.DLSELVIRLEKDAADCQAELTK.T * Jamie_Alpha_SPB_56.20040.20040.2 1.8862 0.1046 95.6 2519.892 2518.79 7782.2 2 3.859 31.0 1 K.DLSELVIRLEKDAADCQAELTK.T * Jamie_Alpha_SPB_14.04885.04885.2 2.7975 0.2385 100.0 1822.9922 1821.9885 6231.8 1 4.881 50.0 1 R.LEKDAADCQAELTKTK.S * Jamie_Alpha_SPB_23.08331.08331.2 2.5435 0.0231 90.9 1765.6522 1762.917 7929.1 1 3.291 64.3 1 K.SSLYSAQDLLDKHER.K * Jamie_Alpha_SPB_27.09684.09684.2 2.0995 0.0076 81.1 1531.4321 1532.6909 7731.8 22 3.016 50.0 1 R.ELISNIEQTESLR.V * Jamie_Alpha_SPB_12.04329.04329.2 2.8179 0.4185 100.0 1612.1322 1613.6812 6038.0 1 7.212 60.7 1 K.VDDTAANNGDKDHLK.L * Jamie_Alpha_SPB_30.10750.10750.2 1.6869 0.0506 85.6 1050.1122 1049.2584 4818.0 490 3.513 56.2 1 K.LVSLFSNLR.H * Jamie_Alpha_SPB_32.11437.11437.2 4.0443 0.2447 100.0 1603.3522 1602.7473 9051.6 1 5.943 79.2 1 K.EYQCSAVIIDEFK.D * Jamie_Alpha_SPB_25.08874.08874.3 3.0937 0.2796 100.0 2191.4043 2192.3965 5419.9 1 4.795 43.8 2 K.NREIYKQLNDRQEEISR.L * Jamie_Alpha_SPB_25.08844.08844.4 2.9592 0.2047 88.5 2192.9365 2192.3965 5538.2 3 3.819 30.2 1 K.NREIYKQLNDRQEEISR.L * Jamie_Alpha_SPB_17.05824.05824.2 3.097 0.3043 100.0 1717.9122 1717.917 6852.8 1 5.892 53.6 1 R.DLIQTKEQVSINSNK.I * Jamie_Alpha_SPB_23.07981.07981.2 2.6182 0.2633 100.0 1530.8322 1529.7114 6751.9 1 5.647 72.7 1 K.ILVYESEMEQCK.Q * Jamie_Alpha_SPB_19.06722.06722.2 2.3697 0.2642 100.0 1813.7722 1814.0297 8375.7 1 4.758 61.5 1 K.ILVYESEMEQCKQR.Y * Jamie_Alpha_SPB_15.05263.05263.2 1.8725 0.0585 87.8 2192.652 2194.4062 7529.7 9 3.859 35.3 1 R.YQDLSQQQKDAQKKDIEK.L * Jamie_Alpha_SPB_40.14250.14250.3 4.8309 0.3762 100.0 3394.8542 3393.776 9691.9 1 6.243 27.7 1 R.YQDLSQQQKDAQKKDIEKLTNEISDLKGK.L * Jamie_Alpha_SPB_36.12940.12940.3 4.4106 0.2617 100.0 2273.6343 2274.5767 7473.8 1 5.043 36.8 1 K.DAQKKDIEKLTNEISDLKGK.L * Jamie_Alpha_SPB_37.13040.13040.2 5.2738 0.4275 100.0 2274.4922 2274.5767 7968.7 1 7.816 57.9 1 K.DAQKKDIEKLTNEISDLKGK.L * Jamie_Alpha_SPB_17.05994.05994.3 3.9208 0.324 100.0 2842.1943 2845.0093 7348.2 1 5.15 25.0 1 N.EIS*DLKGKLSSAENANADLENKFNR.L * Jamie_Alpha_SPB_17.05994.05994.2 4.1568 0.4103 100.0 1895.1322 1894.0079 7348.2 1 7.173 62.5 2 K.LSSAENANADLENKFNR.L * Jamie_Alpha_SPB_22.07900.07900.2 2.3784 0.0257 90.3 1602.4722 1600.8125 6954.4 1 2.936 50.0 1 K.KQQAALTNELNELK.A * Jamie_Alpha_SPB_30.10711.10711.2 2.8388 0.3053 100.0 1898.5521 1900.1417 8551.6 1 7.48 53.3 1 K.AIKDKLEQDLHFENAK.V * Jamie_Alpha_SPB_30.10549.10549.3 3.389 0.1581 98.4 1899.8944 1900.1417 9380.5 1 4.592 41.7 1 K.AIKDKLEQDLHFENAK.V * Jamie_Alpha_SPB_38.13630.13630.2 2.5975 0.2758 100.0 2684.652 2685.0496 7963.7 1 5.69 36.4 1 K.AIKDKLEQDLHFENAKVIDLDTK.L * Jamie_Alpha_SPB_20.07260.07260.2 3.1385 0.3144 100.0 1586.6721 1587.7294 6830.9 1 6.339 66.7 2 K.DKLEQDLHFENAK.V * Jamie_Alpha_SPB_21.07548.07548.3 4.039 0.2223 100.0 1589.7244 1587.7294 5590.3 34 4.378 47.9 1 K.DKLEQDLHFENAK.V * Jamie_Alpha_SPB_33.11811.11811.2 2.2401 0.0322 89.0 2370.9922 2372.6372 6213.5 40 3.312 28.9 1 K.DKLEQDLHFENAKVIDLDTK.L * Jamie_Alpha_SPB_17.05932.05932.1 2.5559 0.2745 100.0 1045.4 1045.2645 6000.0 1 4.648 68.8 1 K.VIDLDTKLK.A * Jamie_Alpha_SPB_13.04492.04492.1 2.8007 0.4557 100.0 1271.41 1271.3292 4716.4 1 7.943 70.0 1 K.AHELQSEDVSR.D * Jamie_Alpha_SPB_13.04566.04566.2 2.3911 0.0567 97.0 1271.7922 1271.3292 5280.9 1 4.506 85.0 1 K.AHELQSEDVSR.D * Jamie_Alpha_SPB_20.07108.07108.2 2.3009 0.1756 99.5 2315.7122 2316.4055 8362.9 1 4.611 41.7 1 K.AHELQSEDVSRDHEKDTYR.T * Jamie_Alpha_SPB_7.02319.02319.2 1.9765 0.1926 99.5 1156.0922 1157.1791 4987.3 1 5.561 65.0 1 K.TANSSSDAFEK.L * Jamie_Alpha_SPB_51.18094.18094.3 2.8369 0.2066 98.3 4335.054 4336.7505 10471.2 3 3.836 18.6 1 K.LQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLR.R * Jamie_Alpha_SPB_52.18451.18451.4 5.7754 0.2724 100.0 4493.4565 4492.938 9265.5 1 5.28 21.8 1 K.LQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Alpha_SPB_50.17907.17907.4 5.4501 0.2717 100.0 3666.5366 3665.9897 7044.8 1 6.329 27.6 2 K.STSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Alpha_SPB_51.18213.18213.3 3.9288 0.3371 100.0 3666.6843 3665.9897 7487.9 1 5.905 29.3 1 K.STSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Alpha_SPB_28.10011.10011.2 2.874 0.2708 100.0 1613.1322 1611.7484 4690.1 1 5.185 77.3 1 K.EWLKEYEDETLR.R * Jamie_Alpha_SPB_22.07632.07632.3 3.4294 0.2577 100.0 1515.2943 1514.7684 5114.9 3 4.939 54.5 1 R.RIKEAEENLKKR.I * Jamie_Alpha_SPB_22.07779.07779.2 2.8323 0.3073 100.0 1515.4521 1514.7684 5424.9 1 5.775 59.1 1 R.RIKEAEENLKKR.I * Jamie_Alpha_SPB_13.04450.04450.1 2.2392 0.1286 100.0 1073.53 1074.2194 5033.6 5 4.724 68.8 1 R.IKEAEENLK.K * Jamie_Alpha_SPB_15.05418.05418.3 2.9557 0.0616 81.7 1357.3744 1358.5809 3537.4 37 3.84 42.5 1 R.IKEAEENLKKR.I * Jamie_Alpha_SPB_16.05478.05478.2 3.1957 0.2672 100.0 1359.0521 1358.5809 7002.2 2 5.246 75.0 1 R.IKEAEENLKKR.I * Jamie_Alpha_SPB_34.11949.11949.3 2.8594 0.19 98.3 2063.6042 2063.405 7880.1 5 4.094 40.0 1 K.IISKRKEELEEEFRKK.L * Jamie_Alpha_SPB_23.08154.08154.2 3.4749 0.4087 100.0 1365.3322 1365.4857 5432.1 1 6.458 88.9 8 K.RKEELEEEFR.K * Jamie_Alpha_SPB_23.08082.08082.3 2.9219 0.2509 100.0 1366.4343 1365.4857 8039.2 4 4.471 47.2 1 K.RKEELEEEFR.K * Jamie_Alpha_SPB_24.08700.08700.3 4.8193 0.2472 100.0 1493.5144 1493.6598 8303.6 1 5.359 55.0 1 K.RKEELEEEFRK.K * Jamie_Alpha_SPB_25.09045.09045.2 2.6011 0.3441 100.0 1494.5322 1493.6598 4142.0 5 4.784 65.0 2 K.RKEELEEEFRK.K * Jamie_Alpha_SPB_30.10611.10611.2 3.9865 0.3077 100.0 1621.0721 1621.8339 6008.0 2 6.156 68.2 3 K.RKEELEEEFRKK.L * Jamie_Alpha_SPB_28.10086.10086.3 3.8514 0.2298 100.0 1621.4644 1621.8339 5378.8 5 5.245 52.3 2 K.RKEELEEEFRKK.L * Jamie_Alpha_SPB_21.07315.07315.2 3.9583 0.2918 100.0 1465.8722 1465.6464 5117.5 1 5.013 90.0 1 R.KEELEEEFRKK.L * Jamie_Alpha_SPB_35.12358.12358.3 4.7592 0.4388 100.0 4164.8345 4165.43 7666.8 1 7.737 21.7 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSKGNSERPSAV.A * Jamie_Alpha_SPB_34.12199.12199.4 5.2167 0.423 100.0 4247.4565 4245.43 6796.8 2 6.121 19.7 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPS*KGNSERPSAV.A * Jamie_Alpha_SPB_39.13940.13940.4 7.1948 0.3147 100.0 4925.7764 4924.3057 7581.2 1 6.784 18.1 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSKGNSERPSAVAGFINQK.N * Jamie_Alpha_SPB_40.14185.14185.2 1.86 0.167 98.7 3106.2122 3106.1536 4504.8 1 4.599 25.9 1 K.ENAGSLTFLDNKGS*GEDAEEELWNSPSK.G * Jamie_Alpha_SPB_27.09577.09577.3 3.7721 0.2875 83.3 3402.2043 3402.4827 4579.3 21 5.206 21.7 1 T.FLDNKGSGEDAEEELWNS*PSKGNSERPSAVA.G * Jamie_Alpha_SPB_22.07737.07737.1 1.3601 0.1679 94.1 1734.52 1735.7582 10285.9 14 3.94 36.7 1 K.GSGEDAEEELWNSPSK.G * Jamie_Alpha_SPB_22.07752.07752.3 2.4034 0.1381 89.0 1736.3644 1735.7582 6184.4 72 3.321 36.7 1 K.GSGEDAEEELWNSPSK.G * Jamie_Alpha_SPB_22.07848.07848.2 5.0391 0.3027 100.0 1737.0922 1735.7582 10255.3 1 6.589 73.3 2 K.GSGEDAEEELWNSPSK.G * Jamie_Alpha_SPB_27.09475.09475.2 2.7132 0.1047 98.6 1815.9922 1815.7582 6950.4 18 3.752 43.3 1 K.GS*GEDAEEELWNSPSK.G * Jamie_Alpha_SPB_24.08358.08358.2 4.2441 0.3206 100.0 1816.1522 1815.7582 10210.6 2 6.386 60.0 3 K.GSGEDAEEELWNSPS*K.G * Jamie_Alpha_SPB_22.07735.07735.2 3.444 0.3605 100.0 1757.2522 1758.7063 5927.4 1 5.7 71.4 1 G.S*GEDAEEELWNSPSK.G * Jamie_Alpha_SPB_18.06499.06499.2 3.8032 0.2731 100.0 1676.2122 1675.8418 7050.9 1 5.915 60.0 1 K.GNSERPSAVAGFINQK.N * Jamie_Alpha_SPB_17.06127.06127.2 4.6654 0.5032 100.0 1686.8722 1686.7906 8433.1 1 8.651 85.7 3 K.NDVSFNDSQSMVTNK.E * Jamie_Alpha_SPB_13.04462.04462.2 1.9978 0.0165 80.5 1320.1721 1319.3708 5352.0 10 2.791 58.3 1 K.ENNIVDSSAAGNK.A * Jamie_Alpha_SPB_31.10890.10890.2 2.6609 0.2381 100.0 2052.612 2054.1777 9552.3 19 5.124 34.2 1 K.AAINFSNVTDLTNNSTDGAK.I * Jamie_Alpha_SPB_28.10087.10087.2 3.8453 0.4922 100.0 1911.9922 1912.0203 8072.3 1 9.349 58.8 1 A.INFSNVTDLTNNSTDGAK.I * Jamie_Alpha_SPB_20.07034.07034.2 3.3023 0.2004 88.9 1799.4321 1798.8608 6323.8 1 4.912 43.8 1 I.NFSNVTDLTNNSTDGAK.I * Jamie_Alpha_SPB_9.03051.03051.1 1.6668 0.3104 100.0 935.39 936.0501 4869.5 1 6.726 62.5 1 K.ITEIGSTSK.R * Jamie_Alpha_SPB_9.02985.02985.2 2.7607 0.2496 100.0 1492.0521 1490.5669 3041.0 1 4.858 76.9 3 K.RPIESGTSSDPDTK.K * Jamie_Alpha_SPB_12.04356.04356.3 3.5621 0.196 100.0 1619.8744 1618.741 7616.4 2 5.471 41.1 1 K.RPIESGTSSDPDTKK.V * Jamie_Alpha_SPB_14.04938.04938.2 2.2096 0.0704 94.9 1846.4122 1846.0476 5934.0 4 3.5 46.9 1 K.RPIESGTSSDPDTKKVK.E YOR257W 10 10 51.6% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * Jamie_Alpha_SPB_25.08899.08899.2 4.1644 0.41 100.0 2210.3123 2210.3196 3631.8 1 6.532 61.1 1 K.NRS*SLQSGPLNSELLEEQK.Q * Jamie_Alpha_SPB_31.11208.11208.2 2.3748 0.0029 85.4 1940.6322 1940.0282 6457.3 9 3.335 40.6 1 R.S*SLQSGPLNSELLEEQK.Q * Jamie_Alpha_SPB_47.16772.16772.2 4.2156 0.3812 100.0 2740.7922 2740.9807 9261.4 1 7.083 37.0 1 R.SSLQSGPLNSELLEEQKQEIYEAF.S * Jamie_Alpha_SPB_38.13616.13616.2 4.1004 0.3206 100.0 1825.3121 1823.9542 6743.0 1 6.232 85.7 1 K.REILDLIDEYDSEGR.H * Jamie_Alpha_SPB_43.15285.15285.2 4.5057 0.5254 100.0 1666.9922 1667.7667 6595.3 1 9.583 80.8 1 R.EILDLIDEYDSEGR.H * Jamie_Alpha_SPB_34.12040.12040.1 1.7823 0.0876 95.0 1381.16 1380.5549 8721.3 1 3.852 55.0 1 K.YDDFYIVMGEK.I * Jamie_Alpha_SPB_22.07854.07854.2 2.7326 0.2817 100.0 1393.8121 1394.4833 6977.0 1 5.837 68.2 1 R.AFQLFDDDHTGK.I * Jamie_Alpha_SPB_29.10305.10305.2 2.3092 0.0226 88.2 1836.0922 1836.0544 9003.4 72 2.636 43.3 1 R.AFQLFDDDHTGKISIK.N * Jamie_Alpha_SPB_22.07938.07938.2 2.4638 0.1121 98.7 1701.3121 1703.8871 6472.1 1 4.128 60.7 1 R.VAKELGETLTDEELR.A * Jamie_Alpha_SPB_23.07990.07990.1 1.7511 0.2329 100.0 1404.55 1405.5016 2340.7 1 4.667 54.5 1 K.ELGETLTDEELR.A YNL225C 20 20 45.6% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * Jamie_Alpha_SPB_35.12648.12648.3 3.9997 0.2967 100.0 3377.3342 3377.4758 4565.9 1 6.155 26.8 1 R.LDS*PVS*ENGEIKDGEPIPQNWLNENHVGK.S * Jamie_Alpha_SPB_17.05972.05972.2 3.8677 0.3578 100.0 2165.392 2166.2983 6682.8 1 6.556 55.9 1 R.DSYEENKSPSMDQMNYAR.N * Jamie_Alpha_SPB_8.02562.02562.2 2.0486 0.2251 99.4 1276.6322 1277.3335 4262.7 466 4.218 50.0 1 K.DKSPIGT*DVHK.K * Jamie_Alpha_SPB_21.07261.07261.2 2.22 0.2754 100.0 1364.2722 1363.4294 5306.0 2 4.123 65.0 1 K.DVPNFIHST*PR.E * Jamie_Alpha_SPB_17.05851.05851.2 1.4799 0.0881 83.5 2966.0923 2966.9226 6465.2 57 2.996 23.1 1 R.ANEQASAQPT*DEHTSPDISIEDCNGAK.I * Jamie_Alpha_SPB_19.06835.06835.2 1.8116 0.0722 91.8 1050.2522 1050.2432 5733.2 6 3.956 68.8 1 K.IFLQNSLSK.E * Jamie_Alpha_SPB_38.13682.13682.2 3.7813 0.4288 100.0 2803.0322 2803.1826 4843.5 1 7.877 37.0 1 K.SNDKTLDNKKKIEEQTVLIENLTK.D * Jamie_Alpha_SPB_30.10845.10845.2 4.1447 0.134 100.0 1788.8722 1787.1069 9027.5 1 5.369 71.4 1 K.KKIEEQTVLIENLTK.D * Jamie_Alpha_SPB_27.09571.09571.2 4.019 0.1741 100.0 1658.8322 1658.9329 8402.9 1 6.072 69.2 1 K.KIEEQTVLIENLTK.D * Jamie_Alpha_SPB_29.10176.10176.2 2.2781 0.1297 98.6 2193.0522 2192.4895 5200.7 3 3.643 38.9 1 K.DLSLNKEMLEKANDTIQTK.H * Jamie_Alpha_SPB_74.26420.26420.2 1.3648 0.0994 80.9 2327.0723 2327.6433 6472.5 21 3.196 27.5 1 K.ANDTIQTKHTALLSLTDSLRK.A * Jamie_Alpha_SPB_78.27948.27948.2 2.6855 0.2666 100.0 2776.892 2778.0845 9219.0 1 5.338 39.1 1 K.AELFEIPIGILFFDLYDSEENSSK.L * Jamie_Alpha_SPB_33.11671.11671.2 2.8131 0.1094 99.0 2120.392 2120.327 7183.2 1 5.032 47.1 1 R.FKNAKAEAEDLRNELENK.K * Jamie_Alpha_SPB_19.06790.06790.2 3.0797 0.1952 90.6 1659.7722 1659.7936 5690.2 2 4.318 57.7 1 A.KAEAEDLRNELENK.K * Jamie_Alpha_SPB_18.06312.06312.2 3.1527 0.288 100.0 1530.3322 1531.6195 6266.6 1 5.416 75.0 1 K.AEAEDLRNELENK.K * Jamie_Alpha_SPB_31.10987.10987.3 3.7517 0.2352 100.0 2531.4844 2531.8438 7916.5 1 4.608 30.0 1 K.AEAEDLRNELENKKIEIQTMR.E * Jamie_Alpha_SPB_12.04191.04191.1 2.0355 0.3242 100.0 974.05 975.0898 3379.1 2 5.864 68.8 1 K.NNTLIGTNK.T * Jamie_Alpha_SPB_31.11088.11088.3 3.5873 0.1228 94.3 2416.6143 2414.7178 7129.0 1 3.907 34.2 1 R.LGSWEKSKEKYETSLKDKEK.M * Jamie_Alpha_SPB_14.04851.04851.1 1.6402 0.1815 100.0 1224.1 1224.2725 4315.8 3 3.824 50.0 1 R.FGNLEGNTSER.I * Jamie_Alpha_SPB_14.04747.04747.2 1.7235 0.1214 95.6 1853.5521 1855.0789 5185.8 23 3.306 42.3 1 R.LHRQVY*SQEMNLKK.F YDR356W 50 58 45.3% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Jamie_Alpha_SPB_17.06039.06039.2 2.2238 0.0489 90.9 2922.9722 2920.033 5624.5 11 3.132 28.8 1 R.NTTQTQVVSPT*KVPNANNGDENEGPVK.K * Jamie_Alpha_SPB_15.05208.05208.3 4.0506 0.0651 85.4 3205.3743 3204.3945 3505.4 1 3.976 29.5 1 R.NTTQTQVVS*PTKVPNANNGDENEGPVKKR.Q * Jamie_Alpha_SPB_39.13938.13938.2 3.64 0.3384 100.0 1862.4321 1861.0148 8514.6 1 5.591 66.7 1 R.LFSEASQFDDSFPEIK.A * Jamie_Alpha_SPB_43.15466.15466.2 1.6812 0.1026 91.0 2773.7122 2773.9727 5045.6 168 3.154 23.9 1 R.LFSEASQFDDSFPEIKANIPPS*PR.S * Jamie_Alpha_SPB_14.04836.04836.2 1.9647 0.0922 96.9 931.77216 931.981 2357.8 271 4.357 57.1 1 K.ANIPPS*PR.S * Jamie_Alpha_SPB_16.05762.05762.2 1.9254 0.1441 98.3 1656.1122 1656.8944 7919.1 7 4.014 53.8 1 K.DVPMSQPLKEQEVR.E * Jamie_Alpha_SPB_28.09938.09938.2 3.0483 0.3001 100.0 2367.2122 2366.633 8202.1 1 6.14 52.6 1 R.HITYANSDISNKELYINEIK.S * Jamie_Alpha_SPB_27.09632.09632.2 5.0779 0.4818 100.0 2400.412 2400.4688 6244.2 1 8.361 63.2 2 K.EKNDTLNNYDTLEEETDDLK.N * Jamie_Alpha_SPB_26.09254.09254.2 3.8243 0.5131 100.0 2669.8123 2670.76 4931.5 1 8.278 52.4 2 K.EKNDTLNNYDTLEEETDDLKNR.L * Jamie_Alpha_SPB_36.12750.12750.2 3.0888 0.3616 100.0 2143.3523 2143.1792 8213.5 1 6.717 44.1 2 K.NDTLNNYDTLEEETDDLK.N * Jamie_Alpha_SPB_29.10142.10142.2 2.6019 0.1191 99.1 2412.8523 2413.4705 8131.4 1 3.717 39.5 2 K.NDTLNNYDTLEEETDDLKNR.L * Jamie_Alpha_SPB_28.09930.09930.2 3.8765 0.1097 99.5 1832.8922 1831.9744 9257.7 1 5.097 73.3 2 K.DQVLELENNSDVQSLK.L * Jamie_Alpha_SPB_19.06579.06579.2 2.66 0.3195 100.0 1079.5721 1079.2103 4868.3 1 6.11 81.2 1 K.SDEMDLQLK.Q * Jamie_Alpha_SPB_25.08883.08883.2 3.399 0.3124 100.0 1487.8121 1488.6812 6446.0 3 6.245 59.1 1 K.RLKDELNELETK.F * Jamie_Alpha_SPB_18.06355.06355.1 1.9382 0.0465 96.0 1333.54 1332.4937 6628.6 1 3.142 55.0 1 R.LKDELNELETK.F * Jamie_Alpha_SPB_31.11078.11078.2 2.4346 0.2431 100.0 3069.5322 3069.3062 6766.4 1 5.282 28.8 1 R.LKDELNELETKFSENGSQSSAKENELK.M * Jamie_Alpha_SPB_31.11184.11184.4 3.8141 0.2423 95.0 3070.4165 3069.3062 5160.2 2 4.366 23.7 1 R.LKDELNELETKFSENGSQSSAKENELK.M * Jamie_Alpha_SPB_33.11596.11596.3 5.0317 0.1564 98.2 3071.9343 3069.3062 7863.7 1 4.369 33.7 2 R.LKDELNELETKFSENGSQSSAKENELK.M * Jamie_Alpha_SPB_29.10213.10213.2 3.3828 0.2866 100.0 1506.3722 1504.6775 6423.5 1 5.103 75.0 2 K.NKIAELEEEISTK.N * Jamie_Alpha_SPB_35.12649.12649.4 3.7397 0.259 94.4 2573.9766 2573.9036 8985.2 1 4.815 29.5 1 K.NKIAELEEEISTKNSQLIAKEGK.L * Jamie_Alpha_SPB_22.07861.07861.3 4.8269 0.2966 100.0 2348.8145 2347.5876 8463.0 1 5.604 39.5 1 R.DSQLGSREEELKKTNDKLQK.D * Jamie_Alpha_SPB_34.12247.12247.3 4.068 0.2526 100.0 2731.4043 2732.0232 6409.8 1 4.105 33.0 1 R.DSQLGSREEELKKTNDKLQKDIR.I * Jamie_Alpha_SPB_27.09573.09573.2 2.8797 0.3456 100.0 2416.9521 2417.6758 7992.5 35 6.043 30.0 1 K.THSESKTITDNELESKDKLIK.I * Jamie_Alpha_SPB_35.12390.12390.4 3.7898 0.21 92.0 3202.8965 3202.5425 8087.1 1 4.058 30.7 1 K.ELKEREFNYKISESKLEDEKTTLNEK.I * Jamie_Alpha_SPB_27.09631.09631.3 3.0733 0.0858 83.5 2546.7544 2546.7905 7744.6 1 3.31 30.0 1 R.EFNYKISESKLEDEKTTLNEK.I * Jamie_Alpha_SPB_16.05682.05682.2 5.2884 0.4166 100.0 1864.1522 1865.0447 7266.4 1 8.566 80.0 1 K.ISESKLEDEKTTLNEK.I * Jamie_Alpha_SPB_40.14102.14102.4 3.8594 0.2997 100.0 2951.5366 2953.2761 6117.9 8 4.926 24.6 1 L.RKDIEEYKESAKDSEDKIEELKIR.I * Jamie_Alpha_SPB_34.12276.12276.3 4.1457 0.3411 100.0 2794.9744 2797.0886 8857.6 450 5.34 23.9 1 R.KDIEEYKESAKDSEDKIEELKIR.I * Jamie_Alpha_SPB_31.11074.11074.4 3.9666 0.2103 91.7 2665.7366 2668.9146 7405.4 1 5.899 34.9 1 K.DIEEYKESAKDSEDKIEELKIR.I * Jamie_Alpha_SPB_31.11059.11059.2 4.6175 0.4054 100.0 2668.5723 2668.9146 6579.4 1 7.848 47.6 1 K.DIEEYKESAKDSEDKIEELKIR.I * Jamie_Alpha_SPB_32.11282.11282.3 4.7231 0.318 100.0 2670.1443 2668.9146 10351.1 1 5.528 33.3 1 K.DIEEYKESAKDSEDKIEELKIR.I * Jamie_Alpha_SPB_19.06900.06900.3 2.8757 0.2051 98.1 1475.4543 1475.639 6284.7 1 5.565 56.8 1 K.DSEDKIEELKIR.I * Jamie_Alpha_SPB_41.14760.14760.4 5.4862 0.2895 100.0 3374.5366 3375.7551 8719.0 1 5.077 30.4 1 R.SKDIKQKDEQISDLTQNLKLQEDEISSLK.S * Jamie_Alpha_SPB_24.08400.08400.2 2.6291 0.1274 99.0 2018.3522 2017.2438 7947.4 1 4.261 50.0 1 K.DIKQKDEQISDLTQNLK.L * Jamie_Alpha_SPB_21.07326.07326.2 2.8044 0.2795 100.0 1659.7122 1660.8217 3399.9 1 5.425 57.7 1 K.QKDEQISDLTQNLK.L * Jamie_Alpha_SPB_42.15081.15081.2 3.0081 0.3429 100.0 1875.4922 1875.0831 9543.4 1 6.71 50.0 1 L.NLENKLIESEDELKSL.R * Jamie_Alpha_SPB_37.13344.13344.3 3.2973 0.272 100.0 2028.7743 2031.2706 6696.2 1 5.303 40.6 1 L.NLENKLIESEDELKSLR.D * Jamie_Alpha_SPB_15.05156.05156.1 2.6152 6.0E-4 95.7 1077.3 1076.1888 9652.1 2 3.122 75.0 1 K.LIESEDELK.S * Jamie_Alpha_SPB_19.06823.06823.2 2.342 0.0652 96.9 1390.5322 1390.5358 5453.1 40 3.956 60.0 1 R.DSQKIEIENWK.R * Jamie_Alpha_SPB_19.06561.06561.3 3.1951 0.1652 98.3 1547.7544 1546.7233 6131.4 115 4.253 40.9 1 R.DSQKIEIENWKR.K * Jamie_Alpha_SPB_15.05396.05396.2 3.9507 0.3691 100.0 1367.5721 1366.4734 7206.5 1 6.093 90.0 1 R.KYNNLSLENDR.L * Jamie_Alpha_SPB_34.12295.12295.4 3.8175 0.2373 95.2 3552.8164 3551.8926 7655.7 1 3.935 22.4 1 K.YNNLSLENDRLLTEKESASDKEREISILNR.K * Jamie_Alpha_SPB_27.09558.09558.3 4.1308 0.3214 100.0 2279.8145 2279.57 7908.5 1 5.835 36.8 1 R.KLDEMDKEKWNLQESKEK.Y * Jamie_Alpha_SPB_27.09502.09502.2 4.0835 0.2726 100.0 2280.3123 2279.57 9285.5 1 4.775 58.8 1 R.KLDEMDKEKWNLQESKEK.Y * Jamie_Alpha_SPB_15.05244.05244.2 3.157 0.2473 100.0 1745.5922 1745.8467 4102.9 1 4.803 65.4 1 R.REKEELNENSNNIR.I * Jamie_Alpha_SPB_28.10002.10002.3 2.5438 0.1026 82.3 2053.5544 2054.1777 6576.4 2 3.717 35.3 1 R.KGEHSLNISLPDDDELDR.D * Jamie_Alpha_SPB_28.10042.10042.2 4.8793 0.2031 100.0 2056.6921 2054.1777 10562.6 1 5.393 67.6 2 R.KGEHSLNISLPDDDELDR.D * Jamie_Alpha_SPB_37.13332.13332.4 4.865 0.2912 100.0 3352.0964 3353.5425 6570.8 1 5.047 32.7 1 R.KGEHSLNISLPDDDELDRDYYNSHVYTR.Y * Jamie_Alpha_SPB_37.13221.13221.3 5.3488 0.3941 100.0 3353.6343 3353.5425 9871.4 1 8.383 29.6 1 R.KGEHSLNISLPDDDELDRDYYNSHVYTR.Y * Jamie_Alpha_SPB_28.10050.10050.2 2.2285 0.2525 99.4 1924.9321 1926.0038 8075.1 14 4.421 37.5 1 K.GEHSLNISLPDDDELDR.D YDR382W 2 2 43.6% 110 11050 4.1 U RPP2B SGDID:S000002790, Chr IV from 1239482-1239814, Verified ORF, "Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * Jamie_Alpha_SPB_45.16066.16066.3 3.2722 0.1455 93.9 4690.344 4689.7534 6171.3 2 4.229 15.4 1 K.FATVPTGGASSAAAGAAGAAAGGDAAEEEKEEEAKEES*DDDMGFGLFD.- * Jamie_Alpha_SPB_40.14220.14220.3 3.7803 0.3111 100.0 3643.6143 3642.621 6709.8 4 6.044 20.0 1 A.AAGAAGAAAGGDAAEEEKEEEAKEES*DDDMGFGLFD.- YKL042W 19 21 42.1% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * Jamie_Alpha_SPB_35.12553.12553.3 3.3625 0.3635 100.0 3435.2043 3435.6619 4656.1 38 5.647 22.2 1 R.LYDDYYNIPYQYSNPTPMNRDYNDVGSR.I * Jamie_Alpha_SPB_27.09620.09620.3 3.8306 0.222 100.0 2482.9443 2482.7124 9852.7 1 5.04 32.5 1 R.DYNDVGSRINADKLVPEEYKR.N * Jamie_Alpha_SPB_28.09883.09883.3 3.8648 0.2113 100.0 2517.7444 2518.7546 8509.4 2 5.112 32.1 1 K.VKDPMVDDDPVSENYDQINVPK.H * Jamie_Alpha_SPB_28.09960.09960.2 4.8879 0.4315 100.0 2518.392 2518.7546 5792.1 1 7.598 57.1 1 K.VKDPMVDDDPVSENYDQINVPK.H * Jamie_Alpha_SPB_47.16729.16729.2 1.9686 0.3541 100.0 1376.6522 1377.5554 7645.8 1 5.937 65.0 1 R.TLS*VLTNYVMR.S * Jamie_Alpha_SPB_41.14731.14731.3 3.7748 0.2921 100.0 2216.5444 2216.4966 5845.9 3 4.906 38.2 1 Y.ESAELKRVEEEIEELKRK.I * Jamie_Alpha_SPB_33.11905.11905.3 3.7804 0.3579 100.0 1800.7743 1801.05 6211.1 1 5.551 44.2 1 A.ELKRVEEEIEELKR.K * Jamie_Alpha_SPB_34.12024.12024.2 2.7675 0.3626 100.0 1801.0721 1801.05 6826.9 5 5.315 46.2 1 A.ELKRVEEEIEELKR.K * Jamie_Alpha_SPB_19.06828.06828.2 3.7017 0.3516 100.0 1430.0122 1430.6011 5375.1 1 7.365 85.0 1 K.RVEEEIEELKR.K * Jamie_Alpha_SPB_16.05665.05665.1 2.9629 0.0598 93.8 1118.3 1118.2262 9274.7 7 4.428 75.0 1 R.VEEEIEELK.R * Jamie_Alpha_SPB_41.14809.14809.2 2.5182 0.2661 91.3 1726.2322 1726.8993 7903.3 1 5.567 61.5 1 R.KLS*LNNQLQELQSM.M * Jamie_Alpha_SPB_20.07252.07252.3 3.3072 0.3342 100.0 3371.8442 3374.3179 3822.0 23 5.172 22.2 1 R.HSSQSSRDYS*PSSDACLECSNDLYEKNR.V * Jamie_Alpha_SPB_26.09211.09211.2 4.2085 0.3091 100.0 2256.7122 2254.2546 7940.0 1 5.364 66.7 1 R.DYSPSSDACLECSNDLYEK.N * Jamie_Alpha_SPB_30.10543.10543.2 1.9127 0.1341 97.6 2333.0923 2334.2546 8502.6 38 3.623 27.8 1 R.DYS*PSSDACLECSNDLYEK.N * Jamie_Alpha_SPB_26.09378.09378.2 4.0396 0.4705 100.0 2334.7322 2334.2546 7768.2 1 7.27 55.6 2 R.DY*SPSSDACLECSNDLYEK.N * Jamie_Alpha_SPB_32.11294.11294.2 2.9151 0.0926 98.6 2412.872 2414.2546 5807.1 1 5.945 50.0 2 R.DYS*PSS*DACLECSNDLYEK.N * Jamie_Alpha_SPB_21.07280.07280.2 2.6864 0.2988 100.0 2523.2122 2524.546 5771.9 1 5.688 37.5 1 R.DYSPSSDACLECSNDLYEKNR.V * Jamie_Alpha_SPB_17.06050.06050.2 2.5445 0.0991 98.3 2149.0522 2148.355 6293.1 1 3.704 52.8 1 R.VKPENNMSETFATPTPNNR.- * Jamie_Alpha_SPB_17.06051.06051.2 2.723 0.3527 100.0 2226.9321 2228.355 5431.6 1 5.794 47.2 1 R.VKPENNMSETFATPT*PNNR.- contaminant_KERATIN02 12 14 33.0% 622 61987 5.2 U no description * Jamie_Alpha_SPB_15.05204.05204.3 3.0229 0.1382 93.1 2757.9543 2761.8628 6360.1 38 3.47 23.1 1 -.MSCRQFSSSY*LTSGGGGGGGLGSGGSIR.S * Jamie_Alpha_SPB_13.04435.04435.1 2.3569 0.4119 100.0 1233.48 1233.2833 4788.0 1 6.688 50.0 1 T.SGGGGGGGLGSGGSIR.S * Jamie_Alpha_SPB_34.12118.12118.2 3.4216 0.2232 100.0 2707.7722 2706.7605 6954.4 1 5.597 33.9 1 R.GGGGSFGYSYGGGSGGGFSASSLGGGFGGGSR.G * Jamie_Alpha_SPB_17.06153.06153.2 2.341 0.0987 98.3 1587.2122 1587.6836 6128.0 2 3.358 65.4 1 K.VQALEEANNDLENK.I * Jamie_Alpha_SPB_60.21416.21416.3 3.9468 0.2657 100.0 3948.3542 3946.3167 8312.5 1 5.338 25.0 1 K.NYSPYYNTIDDLKDQIVDLTVGNNKTLLDIDNTR.M * Jamie_Alpha_SPB_46.16420.16420.2 2.942 0.3669 100.0 3264.8323 3266.413 6492.8 1 6.47 30.4 1 K.DIENQYETQITQIEHEVSSSGQEVQSSAK.E * Jamie_Alpha_SPB_36.12664.12664.2 5.1275 0.3914 100.0 1839.2922 1839.0557 9822.8 1 8.622 73.3 1 R.HGVQELEIELQSQLSK.K * Jamie_Alpha_SPB_33.11920.11920.2 2.7169 0.3832 100.0 1967.2522 1967.2297 6600.8 1 7.261 53.1 1 R.HGVQELEIELQSQLSKK.A * Jamie_Alpha_SPB_46.16327.16327.4 3.6555 0.1797 89.3 3169.7366 3169.4468 8544.4 1 4.049 24.1 1 K.MRLEKEIETYHNLLEGGQEDFESSGAGK.I * Jamie_Alpha_SPB_33.11588.11588.2 2.825 0.1601 99.5 2511.9521 2511.6177 5787.6 1 5.783 40.9 2 K.EIETYHNLLEGGQEDFESSGAGK.I * Jamie_Alpha_SPB_12.04194.04194.2 5.2402 0.3321 100.0 1792.4321 1792.7324 7701.8 1 9.652 59.1 2 R.GGSGGSYGGGGSGGGYGGGSGSR.G * Jamie_Alpha_SPB_12.04196.04196.2 3.6318 0.1911 92.0 1735.4922 1735.6805 7757.4 1 8.699 50.0 1 G.GSGGSYGGGGSGGGYGGGSGSR.G YLR212C 6 6 31.1% 473 52628 4.7 U TUB4 SGDID:S000004202, Chr XII from 566283-564862, reverse complement, Verified ORF, "Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body" * Jamie_Alpha_SPB_31.10992.10992.3 3.1933 0.2288 100.0 3136.1343 3136.3152 7035.0 1 5.376 25.0 1 K.EHAIGTDGLSQLPDSSTERDDDTKPFFR.E * Jamie_Alpha_SPB_58.20594.20594.3 4.9575 0.4624 100.0 4137.4146 4138.457 7775.1 1 8.019 25.7 1 K.IDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDR.Y * Jamie_Alpha_SPB_46.16614.16614.2 5.1096 0.5838 100.0 2321.9722 2322.5742 6314.2 1 10.646 64.3 1 R.LIEDSDATVVFDNASLLNISGK.V * Jamie_Alpha_SPB_46.16564.16564.3 3.2253 0.1137 89.6 2323.3145 2322.5742 8497.7 1 3.817 32.1 1 R.LIEDSDATVVFDNASLLNISGK.V * Jamie_Alpha_SPB_78.28110.28110.4 3.9732 0.336 100.0 6613.4966 6610.9644 10602.2 1 6.327 12.9 1 F.QSMQNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHK.L * Jamie_Alpha_SPB_78.28110.28110.3 4.1816 0.3982 100.0 4960.3745 4960.224 10602.2 1 7.449 19.2 1 R.EVVQSLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHK.L YPL131W 7 9 25.3% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_Alpha_SPB_36.12933.12933.2 1.9714 0.0368 85.4 2209.2322 2208.297 9869.0 126 2.943 28.9 1 K.GVEEVEGEYELTEAVEDGPR.P * Jamie_Alpha_SPB_40.14186.14186.2 3.9616 0.503 100.0 2579.1921 2580.7644 6618.0 1 8.671 50.0 3 K.GVEEVEGEYELTEAVEDGPRPFK.V * Jamie_Alpha_SPB_45.15920.15920.2 2.1651 0.0493 90.5 2589.2522 2591.7217 10213.1 41 3.483 28.6 1 R.SYIFGGHVSQYMEELADDDEER.F * Jamie_Alpha_SPB_24.08401.08401.2 3.4796 0.4369 100.0 1829.2722 1829.8867 7629.4 1 6.919 50.0 1 H.VSQYMEELADDDEER.F * Jamie_Alpha_SPB_47.16766.16766.3 3.4315 0.2466 100.0 3982.0144 3981.1023 7616.4 1 4.531 18.8 1 D.EERFS*ELFKGY*LADDIDADSLEDIYTSAHEAIR.A * Jamie_Alpha_SPB_47.16766.16766.2 4.9026 0.4969 100.0 2655.0122 2654.8035 7616.4 1 8.418 50.0 1 K.GYLADDIDADSLEDIYTSAHEAIR.A * Jamie_Alpha_SPB_47.16792.16792.3 3.6145 0.2345 100.0 2655.6243 2654.8035 5586.0 1 4.837 37.0 1 K.GYLADDIDADSLEDIYTSAHEAIR.A YPL255W 6 6 20.0% 385 45384 6.5 U BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p" * Jamie_Alpha_SPB_37.13350.13350.2 2.0818 0.0532 91.1 1463.2922 1462.5714 7522.6 2 2.669 58.3 1 K.DALFGTDISPS*MK.Y * Jamie_Alpha_SPB_16.05646.05646.2 2.1036 0.2838 100.0 1273.2722 1273.4348 3423.5 4 4.918 65.0 1 R.SLRS*PAPIVPR.E * Jamie_Alpha_SPB_33.11835.11835.2 2.3936 0.2497 90.9 2395.0522 2394.5415 5412.0 2 4.361 38.9 1 Q.MDLNSRDLEDLCEDVREQR.E * Jamie_Alpha_SPB_21.07272.07272.2 2.0423 0.1238 98.0 1851.5521 1850.9371 3955.2 9 3.572 46.7 1 K.VLDQTISHEAELATS*R.E * Jamie_Alpha_SPB_17.05892.05892.2 2.3705 0.1722 99.5 1926.6522 1926.9945 5656.3 56 4.025 41.2 1 R.NNERKDTSAGSNIFSTGQ.- * Jamie_Alpha_SPB_17.05888.05888.2 1.4892 0.1496 94.9 2008.1522 2006.9945 5074.7 86 3.817 35.3 1 R.NNERKDT*SAGSNIFSTGQ.- YPL124W 7 8 18.6% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Jamie_Alpha_SPB_15.05366.05366.2 3.2849 0.348 100.0 1904.3922 1905.9298 4081.4 1 6.151 63.3 1 K.LREENFSSNT*SELGNK.K * Jamie_Alpha_SPB_15.05167.05167.2 2.3338 0.1136 98.3 2035.5922 2034.1039 3319.2 6 3.492 46.9 1 K.LREENFSS*NTSELGNKK.H * Jamie_Alpha_SPB_22.07977.07977.2 1.9031 0.0766 91.0 1637.4521 1636.5829 6363.9 330 3.48 38.5 1 R.EENFSSNT*SELGNK.K * Jamie_Alpha_SPB_18.06391.06391.3 2.4867 0.1263 89.9 1536.8944 1535.7439 3674.4 105 3.473 38.6 1 R.KVKS*PLDDKLRR.Q * Jamie_Alpha_SPB_21.07458.07458.2 4.4266 0.4436 100.0 1923.2322 1923.9855 4705.2 1 7.457 70.0 2 R.SSQIHIENEST*EDILK.I * Jamie_Alpha_SPB_31.11080.11080.2 2.4934 0.2476 90.0 2037.2522 2037.1449 7449.8 1 5.029 46.9 1 R.SSQIHIENES*TEDILKI.L * Jamie_Alpha_SPB_43.15348.15348.2 2.6631 0.2983 100.0 2148.652 2150.3042 6359.3 1 6.399 47.1 1 R.SSQIHIENEST*EDILKIL.S YOL039W 1 1 17.0% 106 10746 4.0 U RPP2A SGDID:S000005399, Chr XV from 254295-254615, Verified ORF, "Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * Jamie_Alpha_SPB_24.08500.08500.2 2.2862 0.2699 89.7 1919.2722 1920.0807 6032.6 2 5.276 41.2 1 L.SALEGKSVDELITEGNEK.L YHL043W 1 1 16.5% 170 19812 8.7 U ECM34 SGDID:S000001035, Chr VIII from 14899-15411, Verified ORF, "Non-essential protein of unknown function" * Jamie_Alpha_SPB_76.27110.27110.3 2.506 0.2458 90.0 3081.3245 3085.4216 8483.1 24 4.315 21.3 1 L.TLIVGWKVFSGIGGRRPSDS*NMDGPQTK.H YLR457C 3 3 16.3% 319 37354 10.2 U NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition" * Jamie_Alpha_SPB_35.12525.12525.2 1.8969 0.1748 98.6 2192.8523 2192.4397 8594.3 1 4.122 37.5 1 R.S*DAFKRKISELKYNKQR.I * Jamie_Alpha_SPB_26.09100.09100.2 2.7783 0.3256 100.0 1637.9722 1637.7869 8767.0 6 5.555 50.0 1 K.LQSLQEALNYSNEK.Y * Jamie_Alpha_SPB_56.20203.20203.2 1.6166 0.0632 80.4 2536.0723 2537.5176 11094.9 317 3.711 25.0 1 R.ETEKEDETLS*PISVDFS*SYLS.- contaminant_KERATIN22 4 4 15.2% 645 65865 8.0 U no description * Jamie_Alpha_SPB_15.05124.05124.1 1.3164 0.2308 100.0 831.53 831.9878 4153.7 126 4.586 43.8 1 R.SLVGLGGTK.S Jamie_Alpha_SPB_18.06228.06228.2 2.771 0.1729 99.4 1476.0922 1476.6726 7594.0 2 5.131 72.7 1 R.FLEQQNQVLQTK.W * Jamie_Alpha_SPB_15.05330.05330.3 4.0457 0.3205 100.0 1392.6244 1392.5083 3202.6 2 6.707 52.3 1 R.SKEEAEALYHSK.Y * Jamie_Alpha_SPB_84.29950.29950.4 2.4895 0.3996 100.0 5755.6567 5749.6694 6744.0 45 2.7 12.0 1 S.VTSSTISSNVASKAAFGGS*GGRGS*SSGGGYSSGSSSYGSGGRQSGSRGGSGGGGSISGGGYGSGG.G YAL038W 6 6 14.6% 500 54545 7.7 U CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration" * Jamie_Alpha_SPB_37.13117.13117.2 3.4525 0.4055 100.0 1726.2722 1725.9365 6038.3 1 7.47 56.2 1 K.GVNLPGTDVDLPALSEK.D * Jamie_Alpha_SPB_17.05980.05980.2 1.6603 0.1195 95.9 1003.0722 1003.14355 4177.6 2 3.966 81.2 1 R.TANDVLTIR.E * Jamie_Alpha_SPB_49.17568.17568.2 2.0529 0.1466 98.7 2523.7122 2523.7378 4239.6 40 3.624 27.1 1 R.AEVSDVGNAILDGADCVMLSGETAK.G * Jamie_Alpha_SPB_48.17322.17322.2 3.2774 0.3666 100.0 2569.132 2570.8174 7012.8 1 8.548 50.0 1 R.GVFPFVFEKEPVSDWTDDVEAR.I * Jamie_Alpha_SPB_48.17318.17318.3 5.0563 0.3395 100.0 2570.5745 2570.8174 7326.9 1 7.772 47.6 1 R.GVFPFVFEKEPVSDWTDDVEAR.I * Jamie_Alpha_SPB_24.08364.08364.2 3.6174 0.4332 100.0 1520.0521 1519.5646 8108.9 1 7.251 66.7 1 K.EPVSDWTDDVEAR.I contaminant_KERATIN03 7 10 13.5% 593 59519 5.2 U no description * Jamie_Alpha_SPB_24.08361.08361.2 2.0037 0.2442 99.4 2210.412 2211.3525 7097.5 159 4.452 26.1 1 R.ISSSKGSLGGGFSSGGFSGGSFSR.G * Jamie_Alpha_SPB_26.09201.09201.2 5.0675 0.5554 100.0 1709.4521 1708.7844 8129.2 1 9.779 66.7 4 K.GSLGGGFSSGGFSGGSFSR.G * Jamie_Alpha_SPB_20.07126.07126.2 2.939 0.415 100.0 1451.4122 1451.4949 5733.4 1 7.469 56.7 1 L.GGGFSSGGFSGGSFSR.G * Jamie_Alpha_SPB_16.05664.05664.1 3.1105 0.2831 100.0 1381.44 1382.4668 7991.8 1 6.351 63.6 1 R.ALEESNYELEGK.I * Jamie_Alpha_SPB_39.13939.13939.3 3.0904 0.2087 98.2 3459.9243 3459.713 9069.6 1 4.359 23.2 1 R.ALEESNYELEGKIKEWYEKHGNSHQGEPR.D * Jamie_Alpha_SPB_35.12561.12561.2 2.8557 0.2942 100.0 2213.0322 2213.4033 7795.1 1 5.524 41.7 1 K.SKELTTEIDNNIEQISSYK.S * Jamie_Alpha_SPB_41.14791.14791.4 6.0372 0.3272 100.0 3197.6565 3198.5115 6565.7 1 6.554 35.9 1 K.SKELTTEIDNNIEQISSYKSEITELRR.N YBR133C 8 8 13.2% 827 95153 6.4 U HSL7 SGDID:S000000337, Chr II from 504281-501798, reverse complement, Verified ORF, "Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p" * Jamie_Alpha_SPB_49.17467.17467.2 2.0997 0.1413 98.7 3170.5923 3170.4595 5950.7 2 4.117 26.9 1 R.FLENVSSHS*PELPSNYDYVLLPITTPR.Y * Jamie_Alpha_SPB_47.16681.16681.2 2.7008 0.3872 100.0 2580.9922 2581.6624 9258.2 1 6.27 35.7 1 K.DTVQDEDDFTVEFSQSSLNEFK.I * Jamie_Alpha_SPB_20.07200.07200.4 2.3831 0.3229 87.5 2959.6565 2965.0752 3395.9 284 4.594 24.6 1 M.S*RIHSDTEQKVWYEWS*LESFIY.L * Jamie_Alpha_SPB_17.05850.05850.2 1.8558 0.078 90.8 1572.6522 1571.7306 6614.7 338 3.123 46.2 1 R.SIVTDDTKTLAHNR.H * Jamie_Alpha_SPB_30.10597.10597.2 3.2996 0.3226 100.0 2809.7922 2811.0374 5745.6 1 6.144 45.7 1 K.ALEPSNELPRHEDLEEDVPEVHVR.V * Jamie_Alpha_SPB_30.10602.10602.3 3.5847 0.2132 100.0 2812.8245 2811.0374 6367.8 1 5.0 32.6 1 K.ALEPSNELPRHEDLEEDVPEVHVR.V * Jamie_Alpha_SPB_23.08203.08203.2 4.835 0.5192 100.0 1704.7522 1703.8058 5965.7 1 9.289 80.8 1 R.HEDLEEDVPEVHVR.V * Jamie_Alpha_SPB_23.08188.08188.3 3.1947 0.212 100.0 1705.3444 1703.8058 6332.8 5 4.697 46.2 1 R.HEDLEEDVPEVHVR.V Reverse_YBR048W 1 1 12.8% 156 17749 10.8 U RPS11B SGDID:S000000252, Chr II from 332829-332873,333385-333810, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps11Ap and has similarity to E. coli S17 and rat S11 ribosomal proteins" Reverse_YDR025W 1 1 12.8% 156 17749 10.8 U RPS11A SGDID:S000002432, Chr IV from 491511-491555,491895-492320, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps11Bp and has similarity to E. coli S17 and rat S11 ribosomal proteins" Jamie_Alpha_SPB_38.13558.13558.2 1.4808 0.099 86.5 2549.7322 2550.8423 4887.2 257 3.067 23.7 1 R.FAPS*VHVPVNKHRKEYRNYK.P YBR191W 1 1 12.5% 160 18242 10.4 U RPL21A SGDID:S000000395, Chr II from 606265-606275,606664-607135, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Bp and has similarity to rat L21 ribosomal protein" YPL079W 1 1 12.5% 160 18274 10.4 U RPL21B SGDID:S000006000, Chr XVI from 406633-406643,407065-407536, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Ap and has similarity to rat L21 ribosomal protein" Jamie_Alpha_SPB_31.11061.11061.3 2.6922 0.3271 80.0 2269.7043 2273.462 5161.7 8 5.553 31.6 1 F.Y*QGKT*GVVYNVTKSSVGVII.N YNL188W 3 3 12.2% 433 50653 9.3 U KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p" * Jamie_Alpha_SPB_34.11948.11948.2 3.0076 0.2639 100.0 1805.0922 1805.936 7498.7 5 5.022 53.6 1 K.IDEDNIENELQFTPK.I * Jamie_Alpha_SPB_69.24633.24633.2 1.6327 0.0773 85.4 2547.672 2547.8342 7280.0 17 3.273 26.2 1 K.EASILRSSLLGQRNVLNT*RNPK.S * Jamie_Alpha_SPB_41.14528.14528.2 2.2907 0.104 98.0 1849.2122 1848.101 6196.0 23 3.652 40.0 1 K.KLDTIIELLKDDTDSK.E YGL075C 4 4 12.1% 387 44585 8.3 U MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p" * Jamie_Alpha_SPB_11.03866.03866.2 2.4351 0.2908 100.0 1424.6122 1425.4551 7989.4 1 5.519 58.3 1 K.NKNTEGAGIST*PR.K * Jamie_Alpha_SPB_12.04335.04335.2 1.6828 0.1569 98.3 995.0722 996.1057 4418.8 28 4.413 71.4 1 K.KLTES*PIK.L * Jamie_Alpha_SPB_23.08010.08010.2 2.9008 0.2211 100.0 1595.5322 1595.6604 7978.2 184 4.656 39.3 1 R.TSDPGS*PLVTGIDQK.A * Jamie_Alpha_SPB_12.04377.04377.1 1.8421 0.2967 100.0 1212.07 1212.2181 4600.0 2 4.993 55.0 1 R.SNANVDDAYSR.V YJR053W 4 4 11.7% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * Jamie_Alpha_SPB_22.07813.07813.2 3.1543 0.2996 100.0 2012.4722 2013.1687 6348.1 1 5.892 46.9 1 N.LALVNPAIREEEEDEER.E * Jamie_Alpha_SPB_21.07287.07287.2 2.6216 0.2476 88.5 2672.9922 2672.7327 4901.1 2 5.035 34.8 1 F.EELEGEAIDETISSNDKESADHPR.F * Jamie_Alpha_SPB_11.03841.03841.2 2.2014 0.1912 99.5 1153.0922 1153.237 6966.4 1 4.684 77.8 1 K.FSDVDTANRK.A * Jamie_Alpha_SPB_20.07246.07246.2 2.4615 0.2915 90.9 1785.1122 1784.8867 7566.3 4 5.076 43.3 1 I.SVDEETIMDESTVNSK.R contaminant_KERATIN07 2 2 11.6% 473 50915 5.5 U no description * Jamie_Alpha_SPB_98.34990.34990.3 2.3048 0.2777 92.3 4080.2043 4078.333 8346.3 72 4.839 13.4 1 G.GGYGGGLGAGVGGGLGAGLGGGFAGGDGLLVGSEKVT*MQHLS*DR.L Jamie_Alpha_SPB_21.07568.07568.2 2.1971 0.1142 98.3 1380.6921 1380.5437 6393.5 38 4.173 55.0 1 K.TRLEQEIATYR.R Reverse_YJR144W 1 1 11.5% 269 30090 9.3 U MGM101 SGDID:S000003905, Chr X from 700797-701606, Verified ORF, "Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage" * Jamie_Alpha_SPB_18.06190.06190.3 1.9102 0.1491 80.4 3579.2644 3579.0566 5161.0 207 3.756 17.5 1 R.TVLKST*VIT*QSRPVLGWGGAGFAKNLIRRYK.I YHR172W 5 14 11.1% 823 96825 6.4 U SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * Jamie_Alpha_SPB_33.11618.11618.2 3.1042 0.246 100.0 2093.612 2092.2227 8231.0 1 4.977 50.0 1 R.YFNDYEPSDPETPIEFK.I * Jamie_Alpha_SPB_23.08314.08314.2 2.3227 0.0941 97.9 1612.9922 1613.6844 6397.1 68 4.558 41.7 1 R.DCDSEEDKNLLFK.M * Jamie_Alpha_SPB_61.21984.21984.3 4.1366 0.401 100.0 2470.0144 2470.8035 7067.9 1 6.627 36.4 1 R.NPTLAVAIQNELEGGLTNIMTNR.C * Jamie_Alpha_SPB_35.12384.12384.2 4.3514 0.1501 100.0 2395.4321 2393.5261 6111.3 1 5.017 54.8 7 R.TNNGGQEDVGYQEDAALELIQK.L * Jamie_Alpha_SPB_33.11888.11888.2 4.1373 0.4199 100.0 1780.7522 1779.8572 7273.4 1 8.075 73.3 4 K.FDFYDSSSVDAAGIER.V YDL038C 1 1 11.1% 583 58523 4.4 U YDL038C SGDID:S000002196, Chr IV from 384078-382327, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_62.22298.22298.4 2.0777 0.3464 83.3 6327.4165 6327.6685 10005.7 15 1.968 10.7 1 P.ASSVVSLTSSYPT*SSSTVTISANSNGSATLAAQTTSIDPVSSIVSSSGATTIIS*SASIDPASSVV.S YOR373W 7 7 10.8% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Jamie_Alpha_SPB_17.05847.05847.2 2.743 0.2463 100.0 2021.8522 2020.1655 7554.5 1 4.92 47.2 1 K.VPSGFSGTTATSHQEAQWK.Q * Jamie_Alpha_SPB_26.09400.09400.2 1.7897 0.1213 95.9 1479.8322 1480.661 6610.5 312 3.737 45.8 1 K.DLSGVLETNTFKR.H * Jamie_Alpha_SPB_13.04540.04540.2 2.7044 8.0E-4 90.0 2172.3523 2172.152 6368.2 15 3.546 41.7 1 K.NNNNNNNNNNNNSININNK.D * Jamie_Alpha_SPB_19.06622.06622.2 1.7745 0.0891 90.5 2337.112 2338.401 4787.7 80 3.38 31.6 1 K.KIQEEENLANSDDTPLDT*PK.F * Jamie_Alpha_SPB_22.07786.07786.2 2.5562 0.163 99.5 2210.132 2210.2268 7178.7 8 4.382 36.1 1 K.IQEEENLANSDDT*PLDTPK.F * Jamie_Alpha_SPB_21.07410.07410.2 3.455 0.3303 100.0 1552.4722 1551.52 5838.6 2 6.827 62.5 1 K.LSGS*PSYDSDWEK.I * Jamie_Alpha_SPB_12.04279.04279.2 1.644 0.1222 95.8 913.1922 913.02136 4221.8 8 4.225 64.3 1 R.NNPITTPR.H YDL014W 2 2 10.4% 327 34465 10.2 U NOP1 SGDID:S000002172, Chr IV from 427361-428344, Verified ORF, "Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin" * Jamie_Alpha_SPB_18.06462.06462.2 2.452 0.1475 99.0 2260.912 2260.5059 7081.4 1 3.732 36.8 1 R.ISVEEPSKEDGVPPTKVEYR.V * Jamie_Alpha_SPB_30.10669.10669.2 3.8203 0.3765 100.0 1729.9722 1730.0142 7784.4 1 6.897 73.1 1 R.IKPLEQLTLEPYER.D YKL054C 6 7 9.8% 738 83973 5.0 U DEF1 SGDID:S000001537, Chr XI from 338397-336181, reverse complement, Verified ORF, "RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII" * Jamie_Alpha_SPB_27.09583.09583.2 1.9222 0.1253 96.9 1770.3922 1770.8943 6847.6 36 3.296 39.3 1 K.QIEQPS*LSSKKTTSR.T * Jamie_Alpha_SPB_36.12891.12891.2 1.9844 0.2553 82.5 3092.892 3093.427 10063.9 1 4.952 30.0 1 A.HFYQQSFPYGQPQYGVAGQYPYQLPK.N * Jamie_Alpha_SPB_16.05524.05524.2 2.4593 0.3288 100.0 2811.5723 2811.8572 8451.1 1 5.892 43.5 1 K.NNYNYYQTQNGQEQQSPNQGVAQH.S * Jamie_Alpha_SPB_17.06138.06138.2 3.2081 0.3233 100.0 2893.5322 2891.8572 8006.1 2 5.57 34.8 1 K.NNYNYYQTQNGQEQQS*PNQGVAQH.S * Jamie_Alpha_SPB_14.05048.05048.3 4.7458 0.3899 100.0 3615.9844 3614.653 9819.2 1 6.66 28.3 1 K.NNYNYYQTQNGQEQQSPNQGVAQHSEDSQQK.Q * Jamie_Alpha_SPB_15.05190.05190.3 5.6796 0.3188 100.0 3695.6643 3694.653 7818.8 1 7.238 30.0 2 K.NNYNYYQTQNGQEQQS*PNQGVAQHSEDSQQK.Q Reverse_YHR003C 1 1 9.8% 429 48883 6.8 U YHR003C SGDID:S000001045, Chr VIII from 111312-110023, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_101.36303.36303.4 2.3327 0.2171 86.2 5218.5366 5216.8037 7370.9 161 4.619 13.0 1 R.FMEEVIY*GVEELGLLSSREKNENLRAMQKQFT*ELISDYFKLR.N YLR153C 4 4 9.2% 683 75492 6.7 U ACS2 SGDID:S000004143, Chr XII from 447576-445525, reverse complement, Verified ORF, "Acetyl-coA synthetase isoform, required for growth on glucose; expressed under anaerobic conditions" * Jamie_Alpha_SPB_24.08398.08398.2 4.0879 0.584 100.0 2460.2522 2461.6038 8921.3 1 10.372 42.9 1 R.HAFANPDKPALIYEADDESDNK.I * Jamie_Alpha_SPB_23.08274.08274.2 2.3022 0.2701 100.0 1334.9722 1335.4178 6323.8 1 6.317 72.2 1 R.DYWWHEEAAK.Q * Jamie_Alpha_SPB_43.15184.15184.2 2.5443 0.0381 93.9 2341.9922 2342.6128 7427.9 2 4.866 31.6 1 R.VLGSVGEPISPDLWEWYHEK.V * Jamie_Alpha_SPB_14.04741.04741.2 2.6746 0.2408 100.0 1196.7922 1197.2932 3541.9 2 4.941 75.0 1 R.VDDVVNVSGHR.L Reverse_YIL022W 2 2 9.0% 431 48854 9.4 U TIM44 SGDID:S000001284, Chr IX from 311163-312458, Verified ORF, "Peripheral mitochondrial membrane protein involved in mitochondrial protein import, tethers essential chaperone Ssc1p to the translocon channel at the matrix side of the inner membrane" * Jamie_Alpha_SPB_29.10494.10494.3 3.109 0.1508 95.3 2274.4143 2271.582 7862.0 88 4.055 31.6 1 K.LTKGVITSGRQAKLY*AERAK.K * Jamie_Alpha_SPB_65.23330.23330.3 2.0974 0.1351 81.3 2275.0144 2271.3835 5919.4 420 2.987 26.4 1 R.ATLTRSSTGSTRIFT*S*RHM.- YBR109C 2 2 8.8% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * Jamie_Alpha_SPB_34.12208.12208.1 2.3532 0.2804 100.0 1509.6 1510.5975 6784.4 1 6.108 54.2 1 K.SNDSEQELLEAFK.V * Jamie_Alpha_SPB_34.12186.12186.2 4.0794 0.4153 100.0 1511.0322 1510.5975 10248.5 1 8.401 79.2 1 K.SNDSEQELLEAFK.V YDR432W 2 2 8.7% 414 45407 5.5 U NPL3 SGDID:S000002840, Chr IV from 1328773-1330017, Verified ORF, "RNA-binding protein that carries poly(A)+ mRNA from the nucleus into the cytoplasm; phosphorylation by Sky1p in the cytoplasm may promote release of mRNAs" * Jamie_Alpha_SPB_24.08371.08371.2 2.9636 0.2979 100.0 1586.4922 1585.6677 6232.7 1 6.048 53.8 1 R.ENSLETTFSSVNTR.D * Jamie_Alpha_SPB_64.22836.22836.2 4.3244 0.4805 100.0 2438.3123 2438.6501 6144.1 1 8.918 54.8 1 R.DFDGTGALEFPSEEILVEALER.L Reverse_YGR159C 1 1 8.7% 414 44535 4.9 U NSR1 SGDID:S000003391, Chr VII from 807661-806417, reverse complement, Verified ORF, "Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis" * Jamie_Alpha_SPB_69.24646.24646.3 1.9455 0.1572 83.3 3634.4043 3630.7603 9749.4 381 3.305 15.7 1 R.GGFGRNGGRGGGRGGFGRS*GGRGGDNNPRPSSFDLR.V contaminant_SPA2_STAAU 4 6 8.5% 508 55439 5.7 U owl|P38507| IMMUNOGLOBULIN G BINDING PROTEIN A PRECURSOR (PROTEIN A). - STAPHYLOCOCCUS... * Jamie_Alpha_SPB_20.07154.07154.1 1.7139 0.0951 94.7 1460.34 1459.5529 3502.7 6 3.248 46.2 2 K.DDPSQSANLLAEAK.K * Jamie_Alpha_SPB_20.07224.07224.2 4.1179 0.3058 100.0 1461.0721 1459.5529 6302.9 1 6.088 84.6 1 K.DDPSQSANLLAEAK.K * Jamie_Alpha_SPB_46.16352.16352.2 3.992 0.4728 100.0 2485.9922 2486.7031 6098.7 1 8.081 44.7 2 K.EQQNAFYEILHLPNLNEEQR.N * Jamie_Alpha_SPB_7.02234.02234.2 3.0985 9.0E-4 98.0 985.09216 985.12823 4596.4 2 4.858 87.5 1 K.KLNDAQAPK.A YLR150W 1 1 8.1% 273 29995 9.7 U STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations" * Jamie_Alpha_SPB_30.10627.10627.3 3.072 0.1311 93.2 2493.5344 2491.801 6970.6 7 4.331 29.8 1 K.KVELDAERIETAEKEAYVPATK.V YGR159C 1 1 8.0% 414 44535 4.9 U NSR1 SGDID:S000003391, Chr VII from 807661-806417, reverse complement, Verified ORF, "Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis" * Jamie_Alpha_SPB_26.09151.09151.3 3.853 0.3459 100.0 3813.0544 3813.9324 8031.4 1 6.15 26.6 1 R.KSEDAEEEEDEESSNKKQKNEETEEPATIFVGR.L YNL126W 3 7 7.8% 846 98227 7.5 U SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * Jamie_Alpha_SPB_63.22402.22402.2 3.6427 0.3538 100.0 2496.5923 2496.8193 9040.7 1 7.898 50.0 4 R.FTEHLEELSGDTFLIELNIFK.S * Jamie_Alpha_SPB_56.20058.20058.3 5.0916 0.4346 100.0 3886.3743 3885.1047 8742.8 1 7.31 29.7 2 R.ATYGEFFIAENTDTNGTDDDFIYHIPIEFNQER.V * Jamie_Alpha_SPB_33.11744.11744.2 2.8344 0.212 100.0 1379.6522 1380.4943 7715.9 1 5.157 63.6 1 K.NDGDEELFLLSK.S Reverse_YPL131W 1 1 7.7% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_Alpha_SPB_95.33940.33940.2 1.2996 0.1741 94.6 2992.4521 2994.3433 7917.6 11 3.442 22.7 1 K.NTFRVVLRYKPTNY*KAKHQT*VLR.K Reverse_YLR150W 1 1 7.7% 273 29995 9.7 U STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex and purine motif triplex nucleic acid; acts with Cdc13p to maintain telomere structure; interacts with ribosomes and subtelomeric Y' DNA; multicopy suppressor of tom1 and pop2 mutations" * Jamie_Alpha_SPB_80.28623.28623.2 1.3465 0.1195 87.4 2589.112 2588.641 8718.9 106 3.696 25.0 1 R.GGRRNNFNNRS*NNNRDGFNKR.T YOR310C 2 2 7.6% 511 56956 8.9 U NOP58 SGDID:S000005837, Chr XV from 898355-896820, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" * Jamie_Alpha_SPB_34.12008.12008.2 3.381 0.3953 100.0 2174.412 2176.339 8641.7 1 7.666 44.4 1 R.SKASETDLSEILPEEIEER.V * Jamie_Alpha_SPB_22.07802.07802.3 3.622 0.281 100.0 2255.5144 2254.3318 6428.3 1 5.94 40.8 1 L.RYDALAEDRDDSGDIGLESR.A Reverse_YOR310C 1 1 7.4% 511 56956 8.9 U NOP58 SGDID:S000005837, Chr XV from 898355-896820, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" * Jamie_Alpha_SPB_16.05798.05798.4 3.6147 0.2683 94.1 4382.0166 4383.39 7514.4 29 4.004 18.9 1 K.KEKKEEEEDDSDS*ESDSAAKATDADANYARAET*MEVKK.A contaminant_KERATIN13 6 6 7.2% 643 65494 6.6 U no description Jamie_Alpha_SPB_18.06228.06228.2 2.771 0.1729 99.4 1476.0922 1476.6726 7594.0 2 5.131 72.7 1 R.FLEQQNQVLQTK.W * Jamie_Alpha_SPB_28.10000.10000.2 3.914 0.4784 100.0 2501.392 2502.7405 8045.9 1 8.382 45.2 1 K.SKAEAESLYQSKYEELQITAGR.H * Jamie_Alpha_SPB_29.10159.10159.3 3.8691 0.2333 100.0 2502.5942 2502.7405 7982.3 1 5.738 33.3 1 K.SKAEAESLYQSKYEELQITAGR.H * Jamie_Alpha_SPB_27.09769.09769.2 2.008 0.1215 97.6 2285.672 2287.4883 5371.1 129 3.615 34.2 1 K.AEAESLYQSKYEELQITAGR.H * Jamie_Alpha_SPB_17.06165.06165.2 2.8747 0.0648 98.6 1180.6921 1180.303 6790.9 1 6.261 94.4 1 K.YEELQITAGR.H * Jamie_Alpha_SPB_19.06643.06643.2 2.7065 0.185 80.6 1454.5322 1454.5797 7029.3 1 5.436 63.6 1 A.TYRTLLEGEESR.M Reverse_YPR116W 1 1 7.2% 277 31133 9.2 U YPR116W SGDID:S000006320, Chr XVI from 758646-759479, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_41.14767.14767.2 1.6975 0.0717 86.2 2609.8323 2607.843 7187.8 93 2.973 26.3 1 K.KRKDSWKEFNT*ILPS*VDKVR.Q YDL224C 1 1 7.1% 649 70690 7.0 U WHI4 SGDID:S000002383, Chr IV from 56347-54398, reverse complement, Verified ORF, "Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division" * Jamie_Alpha_SPB_90.32180.32180.4 2.3015 0.3246 90.0 5070.3364 5074.337 9945.4 253 5.165 13.7 1 G.DELVNDVGNS*SFGASLQEPPMS*STPVMEWNASSTANIPLFQLSSQE.N contaminant_KERATIN05 2 2 7.0% 471 51531 5.2 U no description Jamie_Alpha_SPB_21.07568.07568.2 2.1971 0.1142 98.3 1380.6921 1380.5437 6393.5 38 4.173 55.0 1 K.TRLEQEIATYR.R * Jamie_Alpha_SPB_19.06598.06598.2 2.4297 0.213 99.4 2309.7522 2310.396 8202.8 1 4.154 40.5 1 R.LLEGEDAHLSSSQFSSGSQSSR.D YDL229W 2 2 6.9% 613 66602 5.4 U SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p" YNL209W 2 2 6.9% 613 66595 5.5 U SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; homolog of SSB1" Jamie_Alpha_SPB_15.05212.05212.2 3.3107 0.3762 100.0 1552.3722 1552.7739 5921.6 1 6.691 64.3 1 R.LIGDAAKNQAALNPR.N Jamie_Alpha_SPB_57.20500.20500.2 2.0072 0.2253 99.5 2964.7322 2965.1511 7271.5 4 5.167 25.0 1 R.TLSSVTQTTVEVDSLFDGEDFESSLTR.A Reverse_YLR457C 1 1 6.9% 319 37354 10.2 U NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition" * Jamie_Alpha_SPB_102.36519.36519.2 1.5983 0.1162 91.2 2557.7922 2560.5674 7185.5 96 4.306 26.2 1 R.DKGSSGDSSGSRLES*IRQKNY*K.L YGR192C 2 2 6.9% 332 35747 7.0 U TDH3 SGDID:S000003424, Chr VII from 883815-882817, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " * Jamie_Alpha_SPB_19.06633.06633.2 3.7367 0.4322 100.0 1884.0721 1884.0557 6757.7 1 7.234 56.2 1 R.YAGEVSHDDKHIIVDGK.K * Jamie_Alpha_SPB_5.01811.01811.1 1.1912 0.1142 96.2 725.87 726.80457 7008.9 143 3.764 60.0 1 K.YTSDLK.I YOR204W 3 3 6.6% 604 65553 7.8 U DED1 SGDID:S000005730, Chr XV from 722911-724725, Verified ORF, "ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all yeast mRNAs; mutations in human DEAD-box DBY are a frequent cause of male infertility" * Jamie_Alpha_SPB_16.05668.05668.2 2.5886 0.3446 100.0 1659.4321 1658.7896 5698.3 10 5.757 46.2 1 R.HIVEDCDMTPVGER.Q * Jamie_Alpha_SPB_16.05730.05730.3 2.482 0.1175 89.2 1326.1444 1324.2578 2513.3 1 3.184 45.0 1 R.VGST*SENIT*QK.V * Jamie_Alpha_SPB_61.21866.21866.2 1.8156 0.0607 87.7 1555.9922 1557.576 5046.2 142 3.37 42.9 1 R.DNSFRGGSGWGSDSK.S contaminant_KERATIN18 2 2 6.2% 562 59822 8.0 U no description Jamie_Alpha_SPB_23.08334.08334.2 2.8885 0.1126 99.5 1359.5521 1358.5345 6738.7 4 3.528 63.6 1 K.NKLEGLEDALQK.A * Jamie_Alpha_SPB_65.23408.23408.3 3.6266 0.2754 85.7 2102.6343 2103.0374 5588.7 17 5.333 28.4 1 G.SGLGVGGGFSSSSGRAT*GGGLS*S.V YML048W 1 1 6.2% 403 45870 7.1 U GSF2 SGDID:S000004511, Chr XIII from 178426-179637, Verified ORF, "ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression" * Jamie_Alpha_SPB_23.08026.08026.2 1.5454 0.0744 81.0 2614.3323 2614.6592 7282.5 12 3.335 25.0 1 R.DLTSPSANGAKNS*GGNNNTTDLKTR.L Reverse_YEL015W 1 1 6.0% 551 61340 6.4 U EDC3 SGDID:S000000741, Chr V from 126629-128284, Verified ORF, "Non-essential conserved protein of unknown function, plays a role in mRNA decapping by specifically affecting the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies" * Jamie_Alpha_SPB_35.12634.12634.3 2.913 0.2201 81.0 3658.1343 3654.1736 8341.5 2 4.204 22.7 1 N.LTLGKS*T*AKAIKGQILKGDKLEVQVGFGVFQSM.- YKL104C 2 3 5.9% 717 80047 6.4 U GFA1 SGDID:S000001587, Chr XI from 245017-242864, reverse complement, Verified ORF, "Glutamine-fructose-6-phosphate amidotransferase, catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis" * Jamie_Alpha_SPB_44.15847.15847.2 2.5382 0.0194 91.1 2375.7722 2373.6213 8055.6 1 2.94 40.0 2 K.VDFVDVEFPEENAGQPEIPLK.S * Jamie_Alpha_SPB_44.15772.15772.2 1.7096 0.0651 85.5 2519.9922 2521.7917 6891.6 30 3.597 27.5 1 K.VLFLEDDDLAHIYDGELHIHR.S YJL034W 2 2 5.9% 682 74468 4.9 U KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p" contaminant_GR78_YEAST 2 2 5.9% 682 74468 4.9 U owl|P16474| 78 KD GLUCOSE REGULATED PROTEIN HOMOLOG PRECURSOR (GRP 78) (IMMUNOGLOBULIN... Jamie_Alpha_SPB_12.04044.04044.2 1.5757 0.0893 88.4 1098.7322 1099.1454 6030.1 29 4.238 61.1 1 K.HGIDVSDNNK.A Jamie_Alpha_SPB_37.13340.13340.2 3.5103 0.4938 100.0 3271.0122 3272.1096 8816.3 1 9.1 36.2 1 K.LYGGADGSGAADYDDEDEDDDGDYFEHDEL.- Reverse_YNL182C 1 1 5.8% 555 61773 5.7 U IPI3 SGDID:S000005126, Chr XIV from 297629-295962, reverse complement, Uncharacterized ORF, "Protein required for cell viability; computational analysis of large-scale protein-protein interaction data suggests a possible role in assembly of the ribosomal large subunit" * Jamie_Alpha_SPB_36.12758.12758.4 3.8395 0.277 80.0 3617.8564 3623.0574 6552.7 9 4.142 22.6 1 G.ILLLTGDMSIELCSVNSNLVNECVLQGMAY*LA.D Reverse_YDR375C 1 1 5.7% 456 51108 8.8 U BCS1 SGDID:S000002783, Chr IV from 1226526-1225156, reverse complement, Verified ORF, "Protein of the mitochondrial inner membrane that functions as an ATP-dependent chaperone, required for the assembly of the cytochrome bc(1) complex from the Rip1p and Qcr10p proteins; member of the CDC48/PAS1/SEC18 ATPase family" * Jamie_Alpha_SPB_78.27813.27813.4 3.3926 0.1813 82.8 3098.4165 3103.388 5206.8 446 3.412 22.0 1 R.YLT*TLT*VTEFPSGNAIDIMKASRERK.I YNL175C 1 1 5.5% 403 45667 9.2 U NOP13 SGDID:S000005119, Chr XIV from 308614-307403, reverse complement, Verified ORF, "Protein of unknown function, localizes to the nucleolus and nucleoplasm; contains an RNA recognition motif (RRM) and has similarity to Nop12p, which is required for processing of pre-18S rRNA" * Jamie_Alpha_SPB_26.09151.09151.2 1.7918 0.1031 92.9 2542.372 2539.7712 8031.4 14 3.514 28.6 1 -.MSETELSKEDAVTKKDNEEQVK.K YGL008C 5 6 5.4% 918 99619 5.1 U PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases" * Jamie_Alpha_SPB_45.15922.15922.2 3.631 0.3679 100.0 2653.5322 2653.9092 9294.1 1 6.757 37.0 1 R.IVTEDCFLQIDQSAITGESLAVDK.H * Jamie_Alpha_SPB_16.05724.05724.3 5.0986 0.424 100.0 2294.4243 2295.3806 8398.3 1 8.187 50.0 1 K.TVEEDHPIPEDVHENYENK.V * Jamie_Alpha_SPB_16.05791.05791.2 3.3333 0.471 100.0 2294.4521 2295.3806 7919.0 1 7.675 55.6 2 K.TVEEDHPIPEDVHENYENK.V * Jamie_Alpha_SPB_28.09818.09818.2 3.4377 0.3616 100.0 2778.7922 2778.9458 8430.1 1 6.927 43.5 1 K.TVEEDHPIPEDVHENYENKVAELA.S * Jamie_Alpha_SPB_28.10128.10128.3 5.8041 0.4472 100.0 3020.9644 3022.2114 6785.5 1 8.071 37.0 1 K.TVEEDHPIPEDVHENYENKVAELASR.G Reverse_YAL028W 2 2 5.3% 528 58750 8.8 U FRT2 SGDID:S000000026, Chr I from 92901-94487, Verified ORF, "Tail-anchored endoplasmic reticulum membrane protein, interacts with homolog Frt1p but is not a substrate of calcineurin (unlike Frt1p), promotes growth in conditions of high Na+, alkaline pH, or cell wall stress; potential Cdc28p substrate" * Jamie_Alpha_SPB_39.14040.14040.2 2.0426 0.1881 99.0 1775.3322 1776.0272 6572.1 1 4.366 50.0 1 K.SIMLS*NELSLLSEMK.R * Jamie_Alpha_SPB_21.07484.07484.2 1.7362 0.0468 80.9 1400.8121 1399.2816 6241.5 4 3.161 50.0 1 R.DT*GDIGSGKSS*SK.I YMR024W 1 1 5.1% 390 44000 9.4 U MRPL3 SGDID:S000004626, Chr XIII from 321874-323046, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" * Jamie_Alpha_SPB_21.07426.07426.2 1.5236 0.155 95.9 2328.5923 2330.5596 7093.1 215 3.4 26.3 1 R.S*IIAAIWAVTEQKDSQAVYR.F YBR039W 1 1 5.1% 311 34351 9.3 U ATP3 SGDID:S000000243, Chr II from 315575-316510, Verified ORF, "Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis" * Jamie_Alpha_SPB_71.25291.25291.2 1.4729 0.0845 81.2 2069.0322 2069.211 6949.6 151 3.387 33.3 1 K.T*NPVRTY*ATLKEVEMR.L Reverse_YER014W 1 1 5.0% 539 59703 9.3 U HEM14 SGDID:S000000816, Chr V from 182599-184218, Verified ORF, "Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX" * Jamie_Alpha_SPB_21.07302.07302.3 2.5565 0.2488 98.1 2896.6743 2897.4863 5887.2 166 4.427 22.1 1 R.LKSLFYTFCLGSVGGGVVAVKARPKLK.T YNR015W 1 1 4.9% 384 42816 8.8 U SMM1 SGDID:S000005298, Chr XIV from 653389-654543, Verified ORF, "Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs" * Jamie_Alpha_SPB_31.11116.11116.3 2.9458 0.2241 89.5 2094.2344 2096.2856 6477.7 2 4.389 34.7 1 K.NTNIGGMIAECAERDPTVF.D YNL003C 1 1 4.9% 284 31027 9.6 U PET8 SGDID:S000004948, Chr XIV from 625831-624977, reverse complement, Verified ORF, "S-adenosylmethionine transporter of the mitochondrial inner membrane, member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth" * Jamie_Alpha_SPB_63.22464.22464.2 1.8623 0.1657 98.6 1538.0721 1538.6598 5130.3 222 3.728 42.3 1 R.LQAKGGFFANGGY*K.G YDR097C 2 2 4.8% 1242 140080 5.8 U MSH6 SGDID:S000002504, Chr IV from 643832-640104, reverse complement, Verified ORF, "Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p" * Jamie_Alpha_SPB_101.36136.36136.2 1.4832 0.1173 89.5 2247.1921 2249.2314 10172.8 11 3.896 32.4 1 K.FSYNTSHS*SSPFT*RNISR.D * Jamie_Alpha_SPB_81.29118.29118.3 1.2693 0.2054 81.9 4745.844 4745.419 3302.5 45 3.589 6.1 1 R.PLKMSILVDEATRNVTFLYKMLEGQS*EGSFGMHVASMCGISK.E Reverse_YLR399C 1 1 4.8% 686 76978 6.0 U BDF1 SGDID:S000004391, Chr XII from 921596-919536, reverse complement, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p" * Jamie_Alpha_SPB_101.36314.36314.4 3.629 0.2369 90.5 3189.3765 3191.2627 5988.2 251 4.16 22.4 1 R.SGASTPSLSSADRTVGSTGDLGNSAGSSSEYQR.F Reverse_YKL148C 1 1 4.8% 640 70229 7.5 U SDH1 SGDID:S000001631, Chr XI from 171134-169212, reverse complement, Verified ORF, "Flavoprotein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone" * Jamie_Alpha_SPB_59.21087.21087.3 2.3913 0.2714 85.7 3624.3542 3624.699 7718.3 11 5.08 20.0 1 G.IVVCDY*EHDIIHYKGDASRSAS*GNVSGQTQR.V YAL005C 3 3 4.8% 642 69768 5.1 U SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall" Jamie_Alpha_SPB_15.05358.05358.2 2.1745 0.1566 99.1 1677.4722 1676.6964 5051.9 1 4.293 53.3 1 K.ATAGDTHLGGEDFDNR.L Jamie_Alpha_SPB_14.05055.05055.2 3.3353 0.3562 100.0 1505.2322 1504.5126 5409.1 1 6.779 61.5 1 T.AGDTHLGGEDFDNR.L * Jamie_Alpha_SPB_17.06144.06144.2 4.3317 0.3253 100.0 1618.8922 1619.7258 6484.0 1 7.722 67.9 1 K.NTISEAGDKLEQADK.D contaminant_INT-STD1 2 2 4.8% 607 69271 6.1 U BSA * Jamie_Alpha_SPB_20.07080.07080.2 1.9132 0.067 89.5 1901.7922 1903.0216 11002.7 55 3.034 36.7 1 R.NECFLSHKDDSPDLPK.L * Jamie_Alpha_SPB_50.17702.17702.2 3.124 0.1754 99.4 1570.8121 1568.7258 9202.7 1 4.621 66.7 1 K.DAFLGSFLYEYSR.R contaminant_KERATIN15 4 5 4.8% 629 64511 6.5 U no description * Jamie_Alpha_SPB_14.04822.04822.1 1.0278 0.1049 92.6 1257.59 1258.1173 5488.0 208 3.169 31.8 1 K.T*SGGGSQGFS*GR.S * Jamie_Alpha_SPB_46.16401.16401.1 2.4567 0.2222 100.0 1302.58 1303.4521 11490.9 2 6.05 54.2 1 R.SLDLDSIIAEVGA.Q * Jamie_Alpha_SPB_44.15810.15810.2 3.8114 0.3863 100.0 1304.5922 1303.4521 7050.9 1 7.554 79.2 2 R.SLDLDSIIAEVGA.Q * Jamie_Alpha_SPB_44.15810.15810.3 2.9312 0.2347 92.9 1956.3844 1952.1223 7050.9 4 4.951 32.4 1 R.SLDLDSIIAEVGAQYEDI.A YOL156W 1 1 4.8% 567 62733 8.5 U HXT11 SGDID:S000005516, Chr XV from 25272-26975, Verified ORF, "Putative hexose transporter that is nearly identical to Hxt9p, has similarity to major facilitator superfamily (MFS) transporters and is involved in pleiotropic drug resistance" * Jamie_Alpha_SPB_44.15756.15756.2 1.4702 0.0857 81.6 2732.0122 2729.3403 8293.4 1 3.495 26.9 1 R.IISGLGVGGIAVLSPMLISEVAPKHIR.G Reverse_YMR169C 1 1 4.7% 506 55385 5.7 U ALD3 SGDID:S000004779, Chr XIII from 600871-599351, reverse complement, Verified ORF, "Cytoplasmic aldehyde dehydrogenase, involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose" * Jamie_Alpha_SPB_49.17362.17362.3 3.2214 0.143 92.9 2745.1743 2741.8853 6206.3 1 4.33 28.3 1 K.DLS*LDVHVSKIQLYNDVGTDGSER.G YKR095W 5 5 4.6% 1875 218454 5.2 U MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs" * Jamie_Alpha_SPB_16.05798.05798.2 2.7342 0.1598 99.5 2191.5122 2191.3599 7514.4 1 4.84 44.1 1 R.KLDDLTEEKKETQSNQQR.T * Jamie_Alpha_SPB_15.05204.05204.2 3.261 0.3193 100.0 1838.9722 1838.8334 6360.1 1 5.619 50.0 1 S.SITDEADEDNENLSAKS.S * Jamie_Alpha_SPB_17.05919.05919.2 2.2042 0.1406 99.0 1418.9922 1418.5468 4328.3 49 3.68 55.0 1 K.EREDLLEETRK.S * Jamie_Alpha_SPB_33.11664.11664.2 2.6888 0.1337 99.5 1817.0322 1817.9879 5389.3 5 3.859 46.4 1 K.LESEIENLKEELENK.E * Jamie_Alpha_SPB_22.07879.07879.4 3.8605 0.3515 100.0 2891.1365 2890.0442 10434.2 1 5.932 29.2 1 K.RPIDEVGELKNDEDDTTENINESKK.I YMR055C 1 1 4.6% 306 35028 8.6 U BUB2 SGDID:S000004659, Chr XIII from 387020-386100, reverse complement, Verified ORF, "Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage" * Jamie_Alpha_SPB_37.13161.13161.2 2.7264 0.1752 99.5 1577.7322 1577.8151 7686.3 1 4.143 65.4 1 R.YLILSEGLPISEDK.Q YHR203C 1 1 4.6% 261 29410 10.1 U RPS4B SGDID:S000001246, Chr VIII from 505530-505517,505247-504476, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" YJR145C 1 1 4.6% 261 29410 10.1 U RPS4A SGDID:S000003906, Chr X from 702983-702970,702713-701942, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; identical to Rps4Bp and has similarity to rat S4 ribosomal protein" Jamie_Alpha_SPB_14.04987.04987.2 3.1326 0.2973 100.0 1355.2922 1354.4998 6651.7 1 6.636 63.6 1 R.ITDEEASYKLGK.V YKR055W 1 1 4.5% 291 32186 6.7 U RHO4 SGDID:S000001763, Chr XI from 547858-548733, Verified ORF, "Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely to be involved in the establishment of cell polarity" * Jamie_Alpha_SPB_59.21283.21283.2 1.7425 0.0586 85.5 1618.5521 1620.7748 6916.7 152 3.169 41.7 1 R.SLST*IPSYEQMKR.T YOR313C 1 1 4.4% 338 38591 9.6 U SPS4 SGDID:S000005840, Chr XV from 902869-901853, reverse complement, Verified ORF, "Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage" * Jamie_Alpha_SPB_32.11368.11368.2 1.8136 0.0743 89.0 1739.9122 1740.956 8936.9 12 3.073 42.9 1 K.LPIAYGYHTNSVYNK.N YER069W 1 1 4.3% 863 94869 8.3 U ARG5,6 SGDID:S000000871, Chr V from 295408-297999, Verified ORF, "Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase, which catalyze the 2nd and 3rd steps in arginine biosynthesis; enzymes form a complex with Arg2p" * Jamie_Alpha_SPB_64.22887.22887.3 2.1906 0.2592 85.7 3940.5544 3938.5083 5544.5 8 4.976 16.7 1 K.DKYKLVGNIKSVTKEPIEAS*IKAGALPILTSLAETAS.G Reverse_YMR001C 2 2 4.3% 705 81031 9.0 U CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate" * Jamie_Alpha_SPB_73.26055.26055.2 1.5176 0.1743 96.8 2414.7922 2412.8914 10070.0 7 4.3 30.0 1 K.GRTKILSPPTKCLASLKERKK.K * Jamie_Alpha_SPB_26.09303.09303.2 1.4043 0.1099 88.2 1132.4122 1131.2772 4397.4 285 3.359 50.0 1 K.IPTHVLKS*R.T YIL036W 1 1 4.3% 587 65264 8.0 U CST6 SGDID:S000001298, Chr IX from 285665-287428, Verified ORF, "Basic leucine zipper (bZIP) transcription factor of the ATF/CREB family, activates transcription of genes involved in utilization of non-optimal carbon sources" * Jamie_Alpha_SPB_89.31833.31833.2 1.4955 0.0883 84.4 2523.5723 2521.7185 5947.6 29 3.18 25.0 1 K.IPHSVSDTSAAATTTSTMNNSALSR.S YAR002W 1 1 4.3% 539 59039 9.3 U NUP60 SGDID:S000000063, Chr I from 152259-153878, Verified ORF, "Subunit of the nuclear pore complex (NPC), functions to anchor Nup2p to the NPC in a dynamic process that is controlled by the nucleoplasmic concentration of Gsp1p-GTP; potential Cdc28p substrate" * Jamie_Alpha_SPB_39.13850.13850.2 1.7108 0.0775 87.7 2880.392 2877.862 6546.2 205 3.406 22.7 1 K.EEEY*DET*HETNISNAKLANFFSK.K YAL012W 1 1 4.1% 394 42542 6.5 U CYS3 SGDID:S000000010, Chr I from 130802-131986, Verified ORF, "Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine;" * Jamie_Alpha_SPB_44.15795.15795.2 3.8457 0.4441 100.0 1775.2322 1775.9476 8683.3 1 8.091 66.7 1 R.ISVGIEDTDDLLEDIK.Q Reverse_YPL075W 1 1 3.8% 785 87729 8.3 U GCR1 SGDID:S000005996, Chr XVI from 412251-412258,413010-415359, Verified ORF, "Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p" * Jamie_Alpha_SPB_65.23178.23178.3 2.5337 0.1607 91.8 3144.2944 3147.3826 6949.4 235 4.423 19.8 1 R.KSYPSEGVPSPS*FPSGTAASAIS*GPLPLKR.K YAL047C 1 1 3.7% 622 72105 5.1 U SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization" * Jamie_Alpha_SPB_36.12802.12802.2 4.8728 0.3326 100.0 2616.172 2614.823 10167.1 1 7.177 52.3 1 K.LNEQSHLLDSLELEENSSSVIEK.Q Reverse_YDL224C 2 2 3.7% 649 70690 7.0 U WHI4 SGDID:S000002383, Chr IV from 56347-54398, reverse complement, Verified ORF, "Putative RNA binding protein and partially redundant Whi3p homolog that regulates the cell size requirement for passage through Start and commitment to cell division" * Jamie_Alpha_SPB_81.28981.28981.2 2.0857 0.1615 98.6 1700.3121 1697.6788 6942.8 15 4.105 42.9 1 K.SFASASATS*SCEKQR.S * Jamie_Alpha_SPB_12.04359.04359.2 1.7247 0.0475 85.6 1049.0721 1049.1289 5471.7 1 3.493 81.2 1 R.KINTESNSR.V YJR002W 1 1 3.7% 593 66953 4.7 U MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p" * Jamie_Alpha_SPB_32.11367.11367.2 2.7109 0.1152 99.0 2352.9922 2351.396 7945.2 8 4.649 35.7 1 K.LDLFADEEDEPNAEGVGEASDK.N YKL085W 1 1 3.6% 334 35650 8.5 U MDH1 SGDID:S000001568, Chr XI from 278767-279771, Verified ORF, "Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle" * Jamie_Alpha_SPB_47.16873.16873.2 1.5476 0.0886 87.7 1419.4122 1421.5094 5974.2 224 3.152 40.9 1 K.RAFSST*VANPYK.V YLR442C 1 1 3.5% 978 111360 6.4 U SIR3 SGDID:S000004434, Chr XII from 1022247-1019311, reverse complement, Verified ORF, "Silencing protein that interacts with Sir2p and Sir4p, and histone H3 and H4 tails, to establish a transcriptionally silent chromatin state; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p" * Jamie_Alpha_SPB_49.17348.17348.3 2.507 0.1154 81.5 4109.5444 4107.526 7290.2 75 3.298 18.9 1 K.QSDEYLKRVS*VPVSGQKTNRQVMHKMGVERS*SKR.L YNL233W 1 1 3.5% 892 100590 5.1 U BNI4 SGDID:S000005177, Chr XIV from 211923-214601, Verified ORF, "Targeting subunit for Glc7p protein phosphatase, localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p" * Jamie_Alpha_SPB_87.31303.31303.3 1.8783 0.1682 85.2 3535.1643 3530.828 8037.1 83 3.662 19.2 1 K.KQDTEQIIDAASVLST*ESPLLRKNHDDTPVK.I YOR273C 1 1 3.5% 659 73223 8.2 U TPO4 SGDID:S000005799, Chr XV from 836428-834449, reverse complement, Verified ORF, "Polyamine transport protein, recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane; member of the major facilitator superfamily" * Jamie_Alpha_SPB_31.11026.11026.2 1.536 0.0778 82.3 2424.5522 2424.3618 6801.3 13 3.361 29.5 1 K.NVTNSGNLDSTSS*STGSITEDEK.R YER111C 2 2 3.4% 1093 123806 8.8 U SWI4 SGDID:S000000913, Chr V from 385872-382591, reverse complement, Verified ORF, "DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair" * Jamie_Alpha_SPB_77.27765.27765.3 2.7842 0.1225 88.9 2133.9543 2131.3074 5389.4 152 3.581 30.6 1 K.T*SPGTKITSPSSYNKTPRK.K * Jamie_Alpha_SPB_28.09951.09951.3 2.3432 0.2751 100.0 1819.5543 1821.8528 4703.6 9 4.532 30.9 1 K.KNSSSSTSATTT*AANKKG.K YMR275C 1 1 3.4% 976 109174 8.7 U BUL1 SGDID:S000004888, Chr XIII from 818580-815650, reverse complement, Verified ORF, "Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases" * Jamie_Alpha_SPB_34.12158.12158.3 3.0908 0.1659 96.9 3902.3044 3904.239 6220.3 10 4.092 19.5 1 K.HAPIPHVKPEEES*ILKEY*TSGDLIHGFITIENK.S YKR090W 1 1 3.4% 706 79448 5.2 U PXL1 SGDID:S000001798, Chr XI from 608588-610708, Verified ORF, "LIM domain-containing protein that localizes to sites of polarized growth, required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; has similarity to metazoan paxillin" * Jamie_Alpha_SPB_20.07178.07178.3 2.6596 0.1331 89.3 2842.0444 2841.9187 8374.1 26 3.657 26.1 1 K.S*AYSSVKGESDIYPPPVLENS*ERR.S Reverse_YKR001C 1 1 3.4% 704 78737 7.9 U VPS1 SGDID:S000001709, Chr XI from 442365-440251, reverse complement, Verified ORF, "GTPase required for vacuolar protein sorting, functions in actin cytoskeleton organization via its interaction with Sla1p; required for late Golgi-retention of some proteins including Kex2p; involved in regulating peroxisome biogenesis" * Jamie_Alpha_SPB_76.27220.27220.3 2.4284 0.1349 86.0 2742.4443 2741.0894 7237.3 120 3.446 25.0 1 R.ETMQGT*AKLVPPPS*ELAALKKKNK.T Reverse_YHR091C 1 1 3.3% 643 73694 9.4 U MSR1 SGDID:S000001133, Chr VIII from 286772-284841, reverse complement, Verified ORF, "Mitochondrial arginyl-tRNA synthetase" * Jamie_Alpha_SPB_101.36420.36420.3 2.2423 0.1627 90.8 2464.2244 2463.638 5587.9 114 3.569 27.5 1 R.TVS*WGMAEYLNS*LFGGIITSR.L YHR154W 1 1 3.2% 1070 123017 7.8 U RTT107 SGDID:S000001197, Chr VIII from 402969-406181, Verified ORF, "Protein that interacts with Mms22p and is implicated in Mms22-dependent DNA repair during S phase, damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; has four BRCT domains; has a role in regulation of Ty1 transposition" * Jamie_Alpha_SPB_29.10478.10478.4 2.7059 0.1968 80.0 4021.4565 4017.4875 6914.8 343 3.833 16.7 1 K.ELTVAYTNYFGSQRFYIQRLVEILGGLSTPELT*R.K Reverse_YFR013W 1 1 3.2% 787 90897 7.8 U IOC3 SGDID:S000001909, Chr VI from 169914-172277, Verified ORF, "Member of a complex (Isw1a) with Isw1p that has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; has homology to Esc8p, which is involved in silencing" * Jamie_Alpha_SPB_32.11503.11503.3 2.3544 0.1218 81.4 2973.3245 2975.3745 6675.3 52 3.812 24.0 1 R.LRYLPNSFVKKS*SSKLNEWTTLPGK.K YER173W 1 1 3.2% 659 75727 6.4 U RAD24 SGDID:S000000975, Chr V from 536295-538274, Verified ORF, "Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein" * Jamie_Alpha_SPB_9.02976.02976.2 1.1841 0.2297 97.6 2304.112 2305.5063 3806.6 168 3.972 15.0 1 R.SAITTLQFWAT*SSGSLPISTR.E Reverse_YBR215W 1 1 3.2% 623 67517 9.2 U HPC2 SGDID:S000000419, Chr II from 653441-655312, Verified ORF, "Highly charged, basic protein required for normal cell-cycle regulation of histone gene transcription; mutants display strong synthetic defects with subunits of FACT, a complex that allows RNA Pol II to elongate through nucleosomes" * Jamie_Alpha_SPB_28.10029.10029.3 2.6039 0.2339 85.0 2311.9443 2307.6274 7533.5 208 3.85 28.9 1 A.IT*GDMNQTPLIVKPTLSKNR.P YLL003W 2 2 3.1% 946 112978 9.8 U SFI1 SGDID:S000003926, Chr XII from 143200-146040, Verified ORF, "Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C" * Jamie_Alpha_SPB_16.05624.05624.3 2.4786 0.1203 84.4 2396.4543 2400.5247 5186.6 15 3.3 30.0 1 K.QINLEST*T*GLNGSPTRGKPLR.Y * Jamie_Alpha_SPB_108.38664.38664.1 1.5774 0.188 85.7 971.42 972.17883 5086.6 158 4.671 57.1 1 D.KSPLSRKR.Q Reverse_YAR035W 1 1 3.1% 687 77766 6.9 U YAT1 SGDID:S000000080, Chr I from 190187-192250, Verified ORF, "Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix" * Jamie_Alpha_SPB_57.20319.20319.3 2.6352 0.1061 82.4 2265.3843 2269.3904 7362.8 131 3.429 28.8 1 K.RPITILNASHAAS*ALSSSS*PK.G Reverse_YML027W 1 1 3.1% 385 42739 10.3 U YOX1 SGDID:S000004489, Chr XIII from 221406-222563, Verified ORF, "Homeodomain-containing transcriptional repressor, binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; potential Cdc28p substrate" * Jamie_Alpha_SPB_29.10393.10393.2 1.4721 0.0825 82.7 1353.4122 1353.4722 5323.8 208 3.199 40.9 1 R.SNPST*KVPTLTK.K YAL035W 2 2 3.0% 1002 112268 5.8 U FUN12 SGDID:S000000033, Chr I from 76428-79436, Verified ORF, "GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2" * Jamie_Alpha_SPB_60.21583.21583.2 1.7282 0.0606 84.9 1655.7122 1657.9783 6541.3 7 3.314 42.9 1 R.IVLCGMNGPIVTNIR.A * Jamie_Alpha_SPB_16.05674.05674.2 3.1953 0.1498 99.4 1738.3121 1737.8608 6558.2 57 4.706 42.9 1 K.VDKEAEQYAEQEGIK.I Reverse_contaminant_KERATIN13 1 1 3.0% 643 65494 6.6 U no description * Jamie_Alpha_SPB_13.04452.04452.2 2.453 0.2299 87.9 1541.2922 1541.4911 6694.4 19 4.943 38.9 1 G.SSSGRGGSSGGSSGGGSGR.G Reverse_YNL119W 1 1 3.0% 493 56473 6.4 U NCS2 SGDID:S000005063, Chr XIV from 401042-402523, Uncharacterized ORF, "Protein with a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts" * Jamie_Alpha_SPB_32.11570.11570.2 1.8425 0.0507 85.3 1831.8322 1832.8076 7269.7 74 3.033 35.7 1 K.RS*EVT*APNRASAPCR.Q Reverse_YHR118C 1 1 3.0% 435 50296 7.9 U ORC6 SGDID:S000001160, Chr VIII from 345631-344324, reverse complement, Verified ORF, "Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p" * Jamie_Alpha_SPB_38.13570.13570.2 1.6204 0.1015 90.2 1357.7122 1357.5931 5305.8 397 3.483 41.7 1 K.ATKLESQPKGVAK.N Reverse_YOR296W 1 1 2.9% 1289 147426 8.6 U YOR296W SGDID:S000005822, Chr XV from 870199-874068, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_48.17215.17215.3 2.9011 0.1278 88.6 4275.0244 4271.2554 9893.7 253 4.009 16.7 1 R.SSRSKEHNVNANNLSLSSRT*DSS*ELSYSPEDGEQLDR.Q Reverse_YGR100W 1 1 2.9% 950 109259 5.4 U MDR1 SGDID:S000003332, Chr VII from 690249-693101, Verified ORF, "Cytoplasmic GTPase-activating protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function" * Jamie_Alpha_SPB_54.19354.19354.4 3.7043 0.1953 88.0 2983.3364 2983.0898 5039.6 9 3.862 25.9 1 K.TTISSAAATPT*S*EVSAISSRTAVSDVRR.R Reverse_Q0050 2 2 2.9% 834 96079 9.9 U AI1 SGDID:S000007261, Chr Mito from 13818-16322, Verified ORF, "Reverse transcriptase required for splicing of the COX1 pre-mRNA, encoded by a mobile group II intron within the mitochondrial COX1 gene" * Jamie_Alpha_SPB_35.12487.12487.2 1.6054 0.0939 87.9 1901.7922 1900.2646 7302.8 2 3.462 40.0 1 K.RNMTIMRGTIYDKTAK.L * Jamie_Alpha_SPB_36.12858.12858.2 1.4151 0.1374 93.2 921.4922 920.0074 3482.4 440 3.616 50.0 1 K.SLEINTDK.G Reverse_YEL023C 1 1 2.9% 682 78319 6.6 U YEL023C SGDID:S000000749, Chr V from 110552-108504, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_21.07419.07419.3 2.2676 0.2765 91.7 2085.0544 2081.2903 6036.5 263 4.446 27.6 1 R.SSTGSGQISQLQFIAEIGRT.S YKL103C 1 1 2.9% 514 57093 5.8 U LAP4 SGDID:S000001586, Chr XI from 247326-245782, reverse complement, Verified ORF, "Vacuolar aminopeptidase, often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway" * Jamie_Alpha_SPB_102.36652.36652.2 1.7802 0.0948 91.5 1817.4722 1815.953 5888.9 239 3.28 32.1 1 R.TIDLGIAQLS*MHS*IR.A YBR001C 1 1 2.8% 780 89679 6.5 U NTH2 SGDID:S000000205, Chr II from 241285-238943, reverse complement, Verified ORF, "Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses" * Jamie_Alpha_SPB_45.16076.16076.3 2.6656 0.1274 89.4 2541.2944 2539.86 6315.8 22 3.521 26.2 1 R.RALAACS*PPLPFFNSLKPSEKK.L Reverse_YLR345W 1 1 2.8% 509 58386 6.4 U YLR345W SGDID:S000004337, Chr XII from 820511-822040, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_117.41928.41928.2 1.7108 0.0669 85.9 1787.9521 1787.8004 7229.0 4 3.37 42.3 1 K.ST*PVVCFYDS*PINK.A YER052C 1 1 2.8% 527 58110 6.7 U HOM3 SGDID:S000000854, Chr V from 257957-256374, reverse complement, Verified ORF, "Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis" * Jamie_Alpha_SPB_94.33565.33565.2 1.9891 0.2689 88.2 1735.4722 1737.05 6889.9 13 4.488 39.3 1 K.LRILGSVDITKKLS*I.V Reverse_YJL036W 1 1 2.8% 423 49002 6.5 U SNX4 SGDID:S000003573, Chr X from 378741-380012, Verified ORF, "Sorting nexin, involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX domain; forms complex with Snx41p and Atg20p" * Jamie_Alpha_SPB_17.06081.06081.2 3.1642 0.1705 99.4 1386.3121 1385.474 9146.7 9 4.156 63.6 1 K.DKAKST*LDNKGK.D YJL130C 4 4 2.7% 2214 245123 5.9 U URA2 SGDID:S000003666, Chr X from 172285-165641, reverse complement, Verified ORF, "Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP" * Jamie_Alpha_SPB_48.17137.17137.2 2.7473 0.0266 94.9 2613.0322 2610.7502 7738.2 5 3.636 30.4 1 K.EDYDGLFISNGPGDPSVLDDLSQR.L * Jamie_Alpha_SPB_35.12352.12352.2 2.8184 0.436 100.0 1644.4122 1644.8627 6201.8 3 6.836 46.4 1 K.VLGTPIDTIITTEDR.E * Jamie_Alpha_SPB_5.01631.01631.2 2.8453 0.2127 100.0 965.53217 966.07886 6221.6 2 4.703 85.7 1 R.TFEEAIQK.A * Jamie_Alpha_SPB_17.05961.05961.2 2.509 0.2039 99.4 1646.4521 1646.7539 6107.2 1 5.56 62.5 1 R.VNEIKEEVDYDHR.A YBL066C 1 1 2.7% 1148 127992 7.6 U SEF1 SGDID:S000000162, Chr II from 100117-96671, reverse complement, Verified ORF, "Putative transcription factor, has homolog in Kluyveromyces lactis" * Jamie_Alpha_SPB_98.35154.35154.4 4.1338 0.2761 85.7 3423.6165 3420.5781 5893.6 37 5.36 22.2 1 S.MSHSRTPIASKSNNMTDLHSVVSDPGSSKS*T*.A YCL061C 2 2 2.7% 1096 124326 5.3 U MRC1 SGDID:S000000566, Chr III from 22106-18816, reverse complement, Verified ORF, "S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint" * Jamie_Alpha_SPB_56.20188.20188.2 1.6179 0.1936 98.7 2197.2322 2196.4714 8813.7 186 4.617 29.4 1 R.INEKRVPLLS*SYANNLKR.E * Jamie_Alpha_SPB_69.24825.24825.2 1.8375 0.1454 98.3 1318.4521 1316.4943 4550.2 29 3.884 45.5 1 K.DESVLPISQLSK.A YPL075W 1 1 2.7% 785 87729 8.3 U GCR1 SGDID:S000005996, Chr XVI from 412251-412258,413010-415359, Verified ORF, "Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p" * Jamie_Alpha_SPB_99.35374.35374.3 2.1985 0.1714 92.0 2455.0444 2459.3667 6057.6 52 3.786 27.5 1 K.PPESS*KSEPNDNSPES*KDPEK.N Reverse_YDR323C 1 1 2.7% 515 59443 8.4 U PEP7 SGDID:S000002731, Chr IV from 1114022-1112475, reverse complement, Verified ORF, "Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance" * Jamie_Alpha_SPB_40.14235.14235.2 1.8009 0.111 95.2 1821.3522 1820.947 7583.3 28 3.068 42.3 1 K.SMES*NMKLNHIIS*R.S Reverse_YER111C 1 1 2.6% 1093 123806 8.8 U SWI4 SGDID:S000000913, Chr V from 385872-382591, reverse complement, Verified ORF, "DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair" * Jamie_Alpha_SPB_75.26757.26757.2 1.1604 0.1794 90.9 3213.7322 3214.6096 8652.9 172 3.639 16.7 1 K.RPT*KNYSSPSTIKTGPSTKRLISNKSRK.P YDR285W 1 1 2.6% 875 100035 6.8 U ZIP1 SGDID:S000002693, Chr IV from 1032430-1035057, Verified ORF, "Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate" * Jamie_Alpha_SPB_70.25107.25107.2 1.5407 0.1747 97.6 2837.8123 2837.0789 9778.0 192 3.023 22.7 1 K.ELEEIKNHKRNEPSKKGTQNFT*K.P Reverse_YBL052C 1 1 2.6% 831 97582 6.5 U SAS3 SGDID:S000000148, Chr II from 121877-119382, reverse complement, Verified ORF, "Histone acetyltransferase catalytic subunit of NuA3 complex that acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; sas3 gcn5 double mutation confers lethality" * Jamie_Alpha_SPB_40.14149.14149.2 1.3692 0.0997 81.3 2483.6921 2480.7874 4260.4 111 3.047 26.2 1 K.SQQSILGKKIKNLGKDSRVS*GR.I YMR179W 1 1 2.6% 758 84697 6.0 U SPT21 SGDID:S000004791, Chr XIII from 619857-622133, Verified ORF, "Protein required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p" * Jamie_Alpha_SPB_32.11359.11359.2 1.867 0.1481 98.3 2101.0522 2100.4233 8484.9 133 3.891 23.7 1 K.VSPGIAT*IAKKPASININPK.Q YLR035C 1 1 2.6% 695 78248 8.1 U MLH2 SGDID:S000004025, Chr XII from 214457-212370, reverse complement, Verified ORF, "Protein required for DNA mismatch repair in mitosis and meiosis; involved in the repair of mutational intermediates; forms a complex with Pms1p and Msh1p to repair mismatched DNA" * Jamie_Alpha_SPB_18.06484.06484.2 1.7439 0.1631 98.3 1991.7522 1991.1682 7437.3 6 4.086 35.3 1 K.VFIDVDSTTGGCEYISVK.D YDR292C 1 1 2.6% 621 69278 7.2 U SRP101 SGDID:S000002700, Chr IV from 1045005-1043140, reverse complement, Verified ORF, "Signal recognition particle (SRP) receptor - alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with SRP102p" * Jamie_Alpha_SPB_21.07591.07591.2 1.425 0.1014 85.4 1711.4521 1711.9214 5752.5 258 2.93 33.3 1 K.CDPYVFSIVGVNGVGK.S YGR278W 1 1 2.6% 577 67294 6.9 U CWC22 SGDID:S000003510, Chr VII from 1046737-1048470, Verified ORF, "Essential protein, component of a complex containing Cef1p, has similarity to S. pombe Cwf22p; putative spliceosomal component, based on computational analysis of large-scale protein-protein interaction data" * Jamie_Alpha_SPB_41.14666.14666.2 3.0758 0.3343 100.0 1776.0922 1775.9043 7720.1 6 6.509 42.9 1 R.LTIIQEVEDAEEEEK.K Reverse_YKL215C 1 1 2.5% 1286 140427 6.8 U YKL215C SGDID:S000001698, Chr XI from 30688-26828, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * Jamie_Alpha_SPB_23.08257.08257.3 2.6953 0.1423 89.5 3338.9944 3337.7153 5861.3 135 3.798 19.4 1 K.LIVDT*VRQSTLVNGGVVAAGSRPS*LLSGAPIK.I YOR151C 1 1 2.5% 1224 138751 6.5 U RPB2 SGDID:S000005677, Chr XV from 616671-612997, reverse complement, Verified ORF, "RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit" * Jamie_Alpha_SPB_36.12793.12793.3 3.4874 0.091 88.4 3545.0344 3548.944 5740.6 226 3.871 19.0 1 R.ELKYELIAEES*EDDSESGKVFIGRLPIMLR.S YJL128C 1 1 2.5% 668 72720 9.0 U PBS2 SGDID:S000003664, Chr X from 180021-178015, reverse complement, Verified ORF, "MAP kinase kinase that plays a pivotal role in the osmosensing signal-transduction pathway, activated under severe osmotic stress" * Jamie_Alpha_SPB_82.29284.29284.2 1.6797 0.0841 88.0 1821.8522 1822.8833 6903.8 50 3.352 37.5 1 K.LS*LSSKGIDFSNGSSSR.I YLR187W 2 2 2.4% 1026 114111 8.3 U SKG3 SGDID:S000004177, Chr XII from 524867-527947, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Caf120p and Skg4p" * Jamie_Alpha_SPB_50.18010.18010.2 1.6371 0.0851 88.5 1381.5122 1379.5992 5317.7 10 3.044 50.0 1 K.S*PKLSAPPKVFK.N * Jamie_Alpha_SPB_72.25850.25850.2 1.7563 0.1002 91.9 1810.3722 1811.736 6406.1 295 3.476 37.5 1 K.DRS*KNENRS*PYER.Y Reverse_YDL031W 1 1 2.4% 995 113158 9.3 U DBP10 SGDID:S000002189, Chr IV from 394214-397201, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis" * Jamie_Alpha_SPB_81.28965.28965.3 2.8758 0.1827 96.8 2721.2944 2720.0166 6957.7 47 4.316 25.0 1 K.SWDDFRGSRFSAAIKQGSEGIIYK.K YCR057C 1 1 2.4% 923 103983 5.1 U PWP2 SGDID:S000000653, Chr III from 223223-220452, reverse complement, Verified ORF, "Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis" * Jamie_Alpha_SPB_37.13251.13251.2 1.7942 0.1185 95.9 2329.6921 2328.5068 7506.8 286 3.379 23.8 1 R.FLVSTDGSMEDGAADDDEVLLK.D Reverse_YBL097W 1 1 2.4% 754 86230 4.7 U BRN1 SGDID:S000000193, Chr II from 40828-43092, Verified ORF, "Essential protein required for chromosome condensation, likely to function as an intrinsic component of the condensation machinery, may influence multiple aspects of chromosome transmission and dynamics" * Jamie_Alpha_SPB_55.19520.19520.3 2.7246 0.1607 93.8 2167.7043 2170.538 5999.2 333 3.574 26.5 1 K.RNKIPMDIKTRGKS*AFVK.D YIL009W 1 1 2.4% 694 77947 9.0 U FAA3 SGDID:S000001271, Chr IX from 339341-341425, Verified ORF, "Long chain fatty acyl-CoA synthetase, has a preference for C16 and C18 fatty acids; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery" * Jamie_Alpha_SPB_51.18111.18111.3 2.6248 0.2444 87.5 1931.0944 1931.0636 3923.8 422 4.292 31.2 1 M.VETES*AAIFTDNQLLAK.M Reverse_YDR229W 1 1 2.4% 453 49976 5.0 U IVY1 SGDID:S000002637, Chr IV from 924779-926140, Verified ORF, "Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase" * Jamie_Alpha_SPB_43.15327.15327.2 1.5675 0.0642 81.5 1422.6122 1424.5944 6636.4 14 3.082 55.0 1 R.EQLKLKLS*QEK.S YGL241W 1 1 2.3% 1004 113921 4.7 U KAP114 SGDID:S000003210, Chr VII from 45445-48459, Verified ORF, "Karyopherin, responsible for nuclear import of Spt15p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear" * Jamie_Alpha_SPB_91.32589.32589.2 1.3216 0.1088 82.5 2694.0923 2696.9465 11052.3 257 3.57 20.5 1 K.TQNISTEQNLLSVLCFLTCNDPK.Q YHR027C 1 1 2.3% 993 109492 4.6 U RPN1 SGDID:S000001069, Chr VIII from 164704-161723, reverse complement, Verified ORF, "Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein?protein interactions" * Jamie_Alpha_SPB_36.12982.12982.2 2.9486 0.4362 100.0 2621.912 2622.7249 8591.5 1 6.822 36.4 1 K.NEEAEIEVDEMEVDAEGEEVEVK.A YDR258C 1 1 2.3% 811 91336 8.0 U HSP78 SGDID:S000002666, Chr IV from 974237-971802, reverse complement, Verified ORF, "Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded matrix proteins; component of the mitochondrial proteolysis system" * Jamie_Alpha_SPB_23.08284.08284.2 1.8419 0.0609 87.6 2150.672 2150.3472 9838.9 176 3.485 30.6 1 K.LVVLPNHEEGEVVEEEAEK.- YMR224C 1 1 2.3% 692 77650 5.9 U MRE11 SGDID:S000004837, Chr XIII from 720652-718574, reverse complement, Verified ORF, "Subunit of a complex with Rad50p and Xrs2p (RMX complex) that functions in repair of DNA double-strand breaks and in telomere stability, exhibits nuclease activity that appears to be required for RMX function; widely conserved" * Jamie_Alpha_SPB_73.26095.26095.2 1.9033 0.0462 85.5 1867.6322 1865.9553 8742.3 255 2.912 30.0 1 K.TKTKTRPAAS*TKT*ASR.R Reverse_YDR356W 1 1 2.2% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Jamie_Alpha_SPB_37.13094.13094.2 1.8793 0.0755 90.0 2332.7922 2331.7332 8535.8 350 3.558 25.0 1 R.QNLKSELQTLQAMLSALKGEK.A Reverse_YER047C 1 1 2.2% 897 100332 9.0 U SAP1 SGDID:S000000849, Chr V from 246502-243809, reverse complement, Verified ORF, "Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system" * Jamie_Alpha_SPB_99.35350.35350.2 1.6196 0.1637 97.2 2327.672 2330.3926 7393.1 175 3.665 26.3 1 K.SPKKNQSAGHS*YPKSNS*VPR.S Reverse_YER069W 1 1 2.2% 863 94869 8.3 U ARG5,6 SGDID:S000000871, Chr V from 295408-297999, Verified ORF, "Protein that is processed in the mitochondrion to yield acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase, which catalyze the 2nd and 3rd steps in arginine biosynthesis; enzymes form a complex with Arg2p" * Jamie_Alpha_SPB_60.21448.21448.2 1.3126 0.1129 83.6 2481.9722 2481.8088 8558.0 152 3.52 27.8 1 R.PLYDLLEKIERIKLKT*GY*K.V Reverse_YLR381W 1 1 2.2% 733 84252 8.7 U CTF3 SGDID:S000004373, Chr XII from 879723-881924, Verified ORF, "Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules" * Jamie_Alpha_SPB_105.37762.37762.2 1.5374 0.0776 82.0 1918.0122 1919.055 6525.0 27 3.188 33.3 1 K.FFDPS*VGFLS*KSSLVK.N Reverse_YDR440W 1 1 2.2% 582 66201 9.1 U DOT1 SGDID:S000002848, Chr IV from 1342483-1344231, Verified ORF, "Nucleosomal histone H3-Lys79 methylase, associates with transcriptionally active genes, functions in gene silencing at telomeres, most likely by directly modulating chromatin structure and Sir protein localization" * Jamie_Alpha_SPB_16.05696.05696.2 2.066 0.0939 95.8 1668.9922 1667.7739 6311.3 6 3.418 50.0 1 K.KVDKKT*TT*SSKKR.T Reverse_YOL055C 1 1 2.2% 551 61269 6.2 U THI20 SGDID:S000005416, Chr XV from 226073-224418, reverse complement, Verified ORF, "Hydroxymethylpyrimidine phosphate kinase, involved in the last steps in thiamine biosynthesis; member of a gene family with THI21 and THI22; functionally redundant with Thi21p" * Jamie_Alpha_SPB_60.21566.21566.2 1.5491 0.0848 86.8 1360.5521 1360.6456 5517.9 238 3.208 40.9 1 R.SYARLAPGRKIK.Q YBR118W 1 1 2.2% 458 50033 9.0 U TEF2 SGDID:S000000322, Chr II from 477665-479041, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" YPR080W 1 1 2.2% 458 50033 9.0 U TEF1 SGDID:S000006284, Chr XVI from 700592-701968, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" Jamie_Alpha_SPB_16.05600.05600.2 2.5346 0.0864 98.6 1280.3922 1279.3922 5968.7 1 3.59 77.8 1 R.FDELLEKNDR.R Reverse_YJL113W 2 2 2.1% 1803 207708 8.0 U YJL113W SGDID:S000003649, Chr X from 197834-198919,198921-203246, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" * Jamie_Alpha_SPB_6.02094.02094.3 1.4273 0.2037 84.9 2738.6042 2742.8755 5525.8 255 3.831 13.8 1 K.Y*EHKEKLDPNKS*IAENYYIAR.I * Jamie_Alpha_SPB_33.11872.11872.2 2.5291 0.0081 89.0 2017.0122 2017.1222 8168.2 29 2.894 37.5 1 K.NDTNNHGNKIKIY*GYGK.V Reverse_YCL061C 1 1 2.1% 1096 124326 5.3 U MRC1 SGDID:S000000566, Chr III from 22106-18816, reverse complement, Verified ORF, "S-phase checkpoint protein found at replication forks, required for DNA replication; also required for Rad53p activation during DNA replication stress, where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p; protein involved in replication checkpoint" * Jamie_Alpha_SPB_18.06501.06501.3 2.5023 0.1242 84.3 2708.2144 2712.0413 7622.4 3 3.859 27.3 1 R.S*AKRLTNLLVNHDVKPDKSKNPR.Q YDR430C 1 1 2.1% 989 112180 6.4 U CYM1 SGDID:S000002838, Chr IV from 1328460-1325491, reverse complement, Verified ORF, "Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology" * Jamie_Alpha_SPB_118.42454.42454.2 1.6475 0.1493 96.9 2693.0322 2693.8523 9127.1 273 3.495 25.0 1 R.KY*IVHKPCFTFSIQGS*EEFSK.S Reverse_YJL041W 1 1 2.1% 823 86516 6.3 U NSP1 SGDID:S000003577, Chr X from 365700-365700,365819-368289, Verified ORF, "Essential component of the nuclear pore complex, which mediates nuclear import and export" * Jamie_Alpha_SPB_25.09048.09048.2 1.7452 0.0926 90.5 2083.912 2081.0715 4331.0 5 3.332 37.5 1 K.ISNIQKELS*TSNDDLS*R.L Reverse_YKR090W 1 1 2.1% 706 79448 5.2 U PXL1 SGDID:S000001798, Chr XI from 608588-610708, Verified ORF, "LIM domain-containing protein that localizes to sites of polarized growth, required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; has similarity to metazoan paxillin" * Jamie_Alpha_SPB_23.08041.08041.2 1.7414 0.1075 91.9 1694.5922 1693.8754 6088.3 25 3.336 39.3 1 K.MT*VPVSPAASVNRQR.T contaminant_KERATIN16 1 1 2.1% 534 57265 6.6 U no description * Jamie_Alpha_SPB_16.05532.05532.2 1.6614 0.1496 97.6 1353.3121 1353.5216 3172.7 304 3.489 40.0 1 K.IIGT*TTLNKRR.- Reverse_YLR024C 1 1 2.0% 1872 216777 6.3 U UBR2 SGDID:S000004014, Chr XII from 193282-187664, reverse complement, Verified ORF, "Cytoplasmic ubiquitin-protein ligase (E3)" * Jamie_Alpha_SPB_41.14582.14582.3 2.925 0.1171 88.2 4704.474 4704.9077 9614.1 99 3.171 15.5 1 R.NNNNDGDGDDDY*QINFLLFTRRCFISLSELLLS*CIFDK.I YKR103W 1 1 2.0% 1218 137996 8.8 U NFT1 SGDID:S000001811, Chr XI from 652718-656374, Verified ORF, "Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds." * Jamie_Alpha_SPB_83.29750.29750.3 2.7685 0.2452 88.2 2901.5645 2898.2605 7980.8 3 4.359 26.1 1 F.VSSFLNFVPSLLMPRILS*Y*VNDPK.S YKL157W 1 1 2.0% 935 105585 7.9 U APE2 SGDID:S000001640, Chr XI from 154996-155276,155660-158186, Verified ORF, "Zinc-dependent metallopeptidase yscII, may have a role in obtaining leucine from dipeptide substrates; sequence coordinates have changed since RT-PCR analysis showed that the adjacent ORF YKL158W comprises the 5' exon of APE2/YKL157W" * Jamie_Alpha_SPB_71.25507.25507.3 2.9215 0.1503 94.2 2261.5745 2260.6135 6436.8 123 3.902 26.4 1 K.GASLLRMISKWLGEET*FIK.G Reverse_YBR237W 1 1 2.0% 849 96359 8.1 U PRP5 SGDID:S000000441, Chr II from 691964-694513, Verified ORF, "RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA" * Jamie_Alpha_SPB_59.21235.21235.2 1.5214 0.1204 90.8 2128.372 2127.3313 5932.5 6 3.038 37.5 1 R.DAEDMVVFT*IRKT*SLLK.G YLL008W 1 1 2.0% 752 84843 5.7 U DRS1 SGDID:S000003931, Chr XII from 131728-133986, Verified ORF, "Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles" * Jamie_Alpha_SPB_15.05199.05199.2 1.8406 0.067 88.2 1672.3522 1671.7576 6800.8 20 3.769 46.4 1 K.ADFYAPETEGDEAKK.Q YMR001C 1 1 2.0% 705 81031 9.0 U CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate" * Jamie_Alpha_SPB_31.10987.10987.2 1.7956 0.0445 82.3 1687.9922 1687.8627 7916.5 261 2.835 38.5 1 K.SLLLESMSS*DKIQR.Q Reverse_YDL074C 1 1 2.0% 700 80692 6.4 U BRE1 SGDID:S000002232, Chr IV from 326149-324047, reverse complement, Verified ORF, "E3 ubiquitin ligase for Rad6p, required for the ubiquitination of histone H2B, recruitment of Rad6p to promoter chromatin and subsequent methylation of histone H3 (on L4 and L79), contains RING finger domain" * Jamie_Alpha_SPB_29.10326.10326.2 1.851 0.1196 96.2 1604.1322 1603.8998 6920.4 126 3.59 38.5 1 K.SLTKIEILISDKSR.M YDR376W 1 1 2.0% 493 56237 8.9 U ARH1 SGDID:S000002784, Chr IV from 1226812-1228293, Verified ORF, "Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability" * Jamie_Alpha_SPB_13.04650.04650.2 1.7587 0.0387 83.4 1345.1921 1345.5382 4979.5 96 3.473 55.6 1 K.ELRELWELEK.Y YKL215C 1 1 1.9% 1286 140427 6.8 U YKL215C SGDID:S000001698, Chr XI from 30688-26828, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * Jamie_Alpha_SPB_57.20418.20418.4 3.6996 0.283 88.9 2805.3364 2799.9773 6591.7 88 4.408 22.9 1 L.LKEFS*IRRGSGGKGKY*TGGNGVVRD.V YDR040C 1 1 1.9% 1091 120357 5.6 U ENA1 SGDID:S000002447, Chr IV from 538463-535188, reverse complement, Verified ORF, "P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance" * Jamie_Alpha_SPB_73.26263.26263.2 1.5309 0.1101 89.5 2353.3523 2354.5427 7506.8 21 3.58 30.0 1 R.NGKSET*INSKDVVPGDICLVK.V YHR103W 1 1 1.9% 852 96398 8.0 U SBE22 SGDID:S000001145, Chr VIII from 320417-322975, Verified ORF, "Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth" * Jamie_Alpha_SPB_95.34104.34104.2 2.2699 0.0379 90.3 1751.8121 1749.98 4645.5 33 3.625 40.0 1 K.SMSVASLDQLGKNKDR.E YDR385W 1 1 1.9% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" YOR133W 1 1 1.9% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" Jamie_Alpha_SPB_30.10609.10609.2 3.6644 0.5086 100.0 1775.7722 1775.9221 5271.9 1 8.351 60.0 1 K.STAISLYSEMSDEDVK.E Reverse_YFL009W 1 1 1.9% 779 86090 7.1 U CDC4 SGDID:S000001885, Chr VI from 116139-118478, Verified ORF, "F-box protein required for G1/S and G2/M transition, associates with Skp1p and Cdc53p to form a complex, SCFCdc4, which acts as ubiquitin-protein ligase directing ubiquitination of the phosphorylated CDK inhibitor Sic1p" * Jamie_Alpha_SPB_28.10015.10015.2 1.7463 0.0811 88.4 1893.4321 1893.1985 8412.0 7 3.203 42.9 1 R.LTTRQPVFKPNYWNK.L YPL270W 1 1 1.9% 773 85137 9.1 U MDL2 SGDID:S000006191, Chr XVI from 30482-32803, Verified ORF, "Half-type ATP-binding cassette (ABC) transporter of the inner mitochondrial membrane" * Jamie_Alpha_SPB_61.21771.21771.2 1.8417 0.1069 94.9 1830.9722 1833.1625 7349.9 2 3.376 39.3 1 R.NMLS*RPRLAKLPSIR.F YCR091W 1 1 1.9% 720 81494 8.5 U KIN82 SGDID:S000000687, Chr III from 274400-276562, Verified ORF, "Putative serine/threonine protein kinase, most similar to cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily" * Jamie_Alpha_SPB_82.29331.29331.2 1.8095 0.1918 99.0 1463.9922 1466.4125 6021.7 351 4.069 42.3 1 R.SKSSGTTDAST*SEK.G YDR266C 1 1 1.9% 639 72756 9.0 U YDR266C SGDID:S000002674, Chr IV from 1002017-1000098, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_15.05227.05227.2 2.0128 0.0343 87.8 1277.3722 1277.4203 6619.4 10 3.076 63.6 1 R.ELNTTSGGNKKK.G YPL083C 1 1 1.9% 467 54649 9.1 U SEN54 SGDID:S000006004, Chr XVI from 398102-396699, reverse complement, Verified ORF, "Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p" * Jamie_Alpha_SPB_11.03915.03915.2 1.4384 0.1226 91.0 1166.3322 1169.2394 6831.5 28 3.121 56.2 1 R.S*NLQAITWR.L YER132C 2 2 1.8% 1753 195382 7.8 U PMD1 SGDID:S000000934, Chr V from 430445-425184, reverse complement, Verified ORF, "Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions" * Jamie_Alpha_SPB_56.20004.20004.3 2.145 0.1418 85.1 2220.2344 2220.3967 5558.1 130 3.382 28.1 1 R.VNIACTECS*ISMHRFWK.L * Jamie_Alpha_SPB_92.33021.33021.2 1.6516 0.1082 91.5 1620.7522 1623.6394 6307.2 36 3.47 38.5 1 R.DS*PVASRRSSHSTR.K Reverse_YJR091C 1 1 1.8% 1091 120374 7.0 U JSN1 SGDID:S000003851, Chr X from 598250-594975, reverse complement, Verified ORF, "Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells" * Jamie_Alpha_SPB_32.11474.11474.2 2.2328 0.0071 83.9 2399.3123 2397.579 6529.3 11 2.955 31.6 1 K.VAS*EVSSFEVLAMNLNRLT*R.A Reverse_YDL058W 2 2 1.7% 1790 206451 4.9 U USO1 SGDID:S000002216, Chr IV from 345665-351037, Verified ORF, "Essential protein involved in intracellular protein transport, coiled-coil protein necessary for transport from ER to Golgi; required for assembly of the ER-to-Golgi SNARE complex" * Jamie_Alpha_SPB_25.08862.08862.2 2.3437 0.2265 85.7 1634.1721 1633.8976 7057.5 54 4.376 42.3 1 D.LDTVLLMLDDIESR.D * Jamie_Alpha_SPB_53.18858.18858.2 1.7469 0.0578 84.6 1778.1122 1777.8828 9095.2 108 3.315 36.7 1 K.NVNEDNATTATELKEK.L YKL204W 1 1 1.7% 632 69762 9.5 U EAP1 SGDID:S000001687, Chr XI from 53705-55603, Verified ORF, "eIF4E-associated protein, binds eIF4E and inhibits cap-dependent translation, also functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade" * Jamie_Alpha_SPB_15.05424.05424.2 1.8602 0.1786 99.0 1088.7722 1087.2211 3549.4 95 4.33 55.0 1 K.GISNSTAIAPR.E YGL173C 1 1 1.6% 1528 175459 7.5 U KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonuclease involved in mRNA decay, evolutionarily conserved component of cytoplasmic processing (P) bodies, plays a role in microtubule-mediated processes, filamentous growth, and ribosomal RNA maturation" * Jamie_Alpha_SPB_85.30529.30529.3 2.4686 0.149 88.8 2955.3843 2958.0813 6914.7 64 3.826 22.9 1 K.VIFLGDYAYGGET*T*IDGYSSDRRLK.I YER164W 1 1 1.6% 1468 168240 6.5 U CHD1 SGDID:S000000966, Chr V from 505387-509793, Verified ORF, "Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SILK complexes" * Jamie_Alpha_SPB_22.07783.07783.3 2.8157 0.2453 86.7 2677.2244 2672.9976 3257.8 13 4.406 23.9 1 K.YLKNLINSNYKDDPLKFSLGNNT.P YMR089C 1 1 1.6% 825 93276 7.6 U YTA12 SGDID:S000004695, Chr XIII from 448085-445608, reverse complement, Verified ORF, "Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes" * Jamie_Alpha_SPB_74.26694.26694.2 1.6559 0.0803 87.7 1610.5922 1610.5938 6295.8 322 3.431 37.5 1 R.TKEQGY*FEGNNSR.N YPL085W 1 1 1.5% 2195 241694 5.4 U SEC16 SGDID:S000006006, Chr XVI from 387064-393651, Verified ORF, "COPII vesicle coat protein required for ER transport vesicle budding and autophagosome formation; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p" * Jamie_Alpha_SPB_42.15126.15126.4 4.0574 0.3159 100.0 3543.3364 3546.694 7494.0 69 4.672 18.2 1 S.LPSIENERSSEEQPENISKSAS*SAYLPSTGGLS.L YHR186C 1 1 1.5% 1557 177608 7.8 U KOG1 SGDID:S000001229, Chr VIII from 480672-475999, reverse complement, Verified ORF, "Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors" * Jamie_Alpha_SPB_12.04192.04192.2 1.3303 0.159 92.0 2879.892 2878.2224 1813.0 15 3.677 13.0 1 R.ILFHYNGHGVPKPT*KSGEIWVFNR.G Reverse_YDR464W 1 1 1.5% 1435 161596 8.9 U SPP41 SGDID:S000002872, Chr IV from 1388862-1393169, Verified ORF, "Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3" * Jamie_Alpha_SPB_43.15517.15517.2 1.7538 0.1333 96.6 2552.5122 2551.7817 8815.0 220 3.043 25.0 1 K.NKAWRKKRAVS*AAENAIS*VYK.K Reverse_YFR016C 1 1 1.5% 1233 137698 4.5 U YFR016C SGDID:S000001912, Chr VI from 180735-177034, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_78.27846.27846.2 1.543 0.0992 87.7 2087.5723 2088.1003 8102.0 1 3.601 38.2 1 K.T*EIDEKSTAAVEVDENEK.N YNL087W 1 1 1.5% 1178 132509 8.5 U TCB2 SGDID:S000005031, Chr XIV from 462413-465949, Verified ORF, "Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from the cell surface to intracellular vesicles near vacuole; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud via the mRNA transport system involving She2p" * Jamie_Alpha_SPB_20.07251.07251.3 2.9762 0.2238 88.9 2447.6343 2446.694 5429.8 60 3.906 29.4 1 K.ERIKKEEFRLTEKYDMYS*.Y Reverse_YNL163C 1 1 1.5% 1110 124463 5.1 U RIA1 SGDID:S000005107, Chr XIV from 330075-326743, reverse complement, Verified ORF, "Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongation factor 2 (Eft1p and Eft2p)" * Jamie_Alpha_SPB_16.05472.05472.2 1.8551 0.1023 93.9 2149.2922 2148.2542 7095.1 16 3.725 31.2 1 R.TS*T*ILRGSLDVIHQQYR.P Reverse_YBR203W 1 1 1.5% 924 104504 9.6 U COS111 SGDID:S000000407, Chr II from 629163-631937, Verified ORF, "Protein required for wild-type resistance to the antifungal drug ciclopirox olamine; not related to the COS family of subtelomerically-encoded proteins" * Jamie_Alpha_SPB_68.24445.24445.2 1.8052 0.0385 80.2 1717.6522 1719.9791 7443.1 96 3.293 34.6 1 K.SST*SILSKYKKKIR.S Reverse_YIL149C 1 1 1.4% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_Alpha_SPB_92.33082.33082.2 1.5221 0.1817 97.6 3014.2922 3014.205 5519.1 14 3.912 21.7 1 K.KEKCCQEFDRTLSEKTPIFS*ATEK.K YOR341W 1 1 1.4% 1664 186431 7.1 U RPA190 SGDID:S000005868, Chr XV from 960982-965976, Verified ORF, "RNA polymerase I subunit; largest subunit of RNA polymerase I" * Jamie_Alpha_SPB_83.29779.29779.2 1.3838 0.109 85.5 2640.5923 2638.9934 8545.7 247 3.079 22.7 1 K.QIITT*VLLNVT*PPDMPGINLISK.N Reverse_YDL223C 1 1 1.4% 1046 113616 6.4 U HBT1 SGDID:S000002382, Chr IV from 60406-57266, reverse complement, Verified ORF, "Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis" * Jamie_Alpha_SPB_87.31201.31201.2 2.1576 0.3206 95.2 1636.0122 1633.672 5055.7 6 5.098 42.9 1 K.VST*HGHTVSPNTKTS.S Reverse_YOL070C 1 1 1.4% 501 55923 5.8 U YOL070C SGDID:S000005431, Chr XV from 198724-197219, reverse complement, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, and bud neck; potential Cdc28p substrate" * Jamie_Alpha_SPB_13.04410.04410.2 1.6473 0.0364 82.4 896.9522 897.9639 3480.4 20 3.492 83.3 1 R.KLNS*TVR.S Reverse_YNL172W 1 1 1.3% 1748 196143 6.0 U APC1 SGDID:S000005116, Chr XIV from 310638-315884, Verified ORF, "Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition" * Jamie_Alpha_SPB_81.28918.28918.2 1.4383 0.0992 85.2 2265.9922 2263.5132 9563.7 184 3.632 26.2 1 K.QSNGAADLGTKKTSSITLEGKR.S Reverse_YPR184W 1 1 1.3% 1536 174971 5.8 U GDB1 SGDID:S000006388, Chr XVI from 902040-906650, Verified ORF, "Glycogen debranching enzyme containing glucanotranferase and alpha-1,6-amyloglucosidase activities, required for glycogen degradation" * Jamie_Alpha_SPB_71.25512.25512.3 2.39 0.1336 87.2 2356.4644 2355.745 5776.2 187 3.08 27.6 1 K.MRSVMQIS*TMSLSQVFLTSK.G YLR450W 1 1 1.3% 1045 115692 7.6 U HMG2 SGDID:S000004442, Chr XII from 1032623-1035760, Verified ORF, "One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks" * Jamie_Alpha_SPB_57.20269.20269.2 1.7066 0.1054 92.0 1535.7522 1538.7029 5987.5 130 3.394 38.5 1 R.T*TTGDAMGMNMISK.G Reverse_YOR132W 1 1 1.3% 551 63204 6.9 U VPS17 SGDID:S000005658, Chr XV from 573175-574830, Verified ORF, "Subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation" * Jamie_Alpha_SPB_24.08700.08700.2 1.8615 0.0084 84.1 996.0122 994.1386 8303.6 4 3.262 83.3 1 K.REYEIKR.L Reverse_YNL250W 1 1 1.2% 1312 152568 6.1 U RAD50 SGDID:S000005194, Chr XIV from 175411-179349, Verified ORF, "Subunit of MRX complex, with Mre11p and Xrs2p, involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining" * Jamie_Alpha_SPB_41.14750.14750.2 1.5737 0.1032 89.2 2135.8323 2136.089 9251.0 56 3.316 30.0 1 R.S*CLYCCS*DREAIELAK.R Reverse_YKL209C 1 1 1.2% 1290 144765 8.0 U STE6 SGDID:S000001692, Chr XI from 46296-42424, reverse complement, Verified ORF, "ATP-binding cassette (ABC) transporter required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport, contains 12 transmembrane domains and two ATP binding domains, expressed only in MATa cells" * Jamie_Alpha_SPB_15.05151.05151.2 1.7068 0.0863 88.7 2196.612 2199.3066 6319.9 33 3.146 35.7 1 R.Y*DNRINVYRFIHY*HK.T Reverse_YNL273W 1 1 1.2% 1238 141120 6.1 U TOF1 SGDID:S000005217, Chr XIV from 122883-126599, Verified ORF, "Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase" * Jamie_Alpha_SPB_59.21031.21031.2 1.7833 0.1551 97.9 1736.7722 1736.1075 7869.4 9 3.42 39.3 1 R.PISYGILLLLARYDK.D YML091C 1 1 1.2% 1202 139348 6.8 U RPM2 SGDID:S000004556, Chr XIII from 90731-87123, reverse complement, Verified ORF, "Protein component of mitochondrial RNase P, along with the mitochondrially-encoded RNA subunit RPM1; Rnase P removes 5' extensions from tRNA precursors; Rpm2p is also involved in maturation of RPM1 and in translation of mitochondrial mRNAs" * Jamie_Alpha_SPB_87.31359.31359.2 1.6729 0.0664 84.7 1824.0122 1823.9626 6281.6 56 3.258 35.7 1 K.SFIYSKGYHRS*AAQK.K Reverse_YBR059C 1 1 1.2% 1108 123989 7.7 U AKL1 SGDID:S000000263, Chr II from 360185-356859, reverse complement, Verified ORF, "Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization" * Jamie_Alpha_SPB_73.26301.26301.2 1.671 0.1116 92.3 1543.0122 1543.5039 4751.1 191 3.648 37.5 1 K.NET*IAT*SAHPSVR.Y Reverse_YER114C 1 1 1.2% 1040 115688 8.8 U BOI2 SGDID:S000000916, Chr V from 393708-390586, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" * Jamie_Alpha_SPB_29.10172.10172.2 1.6682 0.0632 84.9 1595.9321 1596.571 7156.8 28 3.145 45.8 1 R.S*AS*SSRKLSSNRR.S YPR160W 1 1 1.2% 902 103275 5.6 U GPH1 SGDID:S000006364, Chr XVI from 861302-864010, Verified ORF, "Non-essential glycogen phosphorylase required for the mobilization of glycogen, activity is regulated by cyclic AMP-mediated phosphorylation, expression is regulated by stress-response elements and by the HOG MAP kinase pathway" * Jamie_Alpha_SPB_13.04639.04639.2 1.9734 0.0709 92.9 1375.2122 1374.4906 4558.4 54 3.198 55.0 1 K.LTQLGKY*VEDK.E Reverse_YKR099W 1 1 1.2% 811 89604 8.2 U BAS1 SGDID:S000001807, Chr XI from 635489-637924, Verified ORF, "Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways" * Jamie_Alpha_SPB_109.39201.39201.1 1.4561 0.1955 92.9 1090.21 1091.2523 6684.8 1 4.405 55.6 1 K.SGPGLVEIYR.K YLL040C 1 1 1.1% 3144 357850 5.6 U VPS13 SGDID:S000003963, Chr XII from 63644-54210, reverse complement, Verified ORF, "Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention" * Jamie_Alpha_SPB_117.41900.41900.4 3.8443 0.3129 100.0 3930.0166 3928.2158 8022.9 28 4.635 20.1 1 N.S*SVTDGFVQKDEDEKVNTQIVVSFKGVGIS*LINGR.L YGR027W-B 2 2 1.1% 1755 198656 8.2 U YGR027W-B SGDID:S000007406, Chr VII from 536061-537365,537367-541329, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YPR158C-D 2 2 1.1% 1755 198373 7.9 U YPR158C-D SGDID:S000007362, Chr XVI from 856253-854949,854947-850985, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YJR029W 2 2 1.1% 1755 198616 8.3 U YJR029W SGDID:S000003790, Chr X from 478258-479558,479560-483526, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Alpha_SPB_13.04401.04401.2 5.1018 0.5488 100.0 2075.2122 2076.098 5108.7 1 11.353 65.8 1 R.AHNVSTSNNSPSTDNDSISK.S Jamie_Alpha_SPB_12.04334.04334.2 3.4241 0.2304 100.0 2157.0923 2156.098 4635.9 4 5.693 44.7 1 R.AHNVSTSNNSPST*DNDSISK.S Reverse_YHR164C 1 1 1.1% 1522 171694 6.5 U DNA2 SGDID:S000001207, Chr VIII from 429180-424612, reverse complement, Verified ORF, "Essential tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair pathways; potential Cdc28p substrate" * Jamie_Alpha_SPB_28.09873.09873.2 2.1029 0.0484 89.7 1878.7722 1878.9481 7670.4 143 2.906 33.3 1 K.EETKKAPSVSIS*AS*RK.N Reverse_YGR270W 1 1 1.1% 1379 157407 5.2 U YTA7 SGDID:S000003502, Chr VII from 1027376-1031515, Verified ORF, "Protein of unknown function, member of CDC48/PAS1/SEC18 family of ATPases, potentially phosphorylated by Cdc28p" * Jamie_Alpha_SPB_92.33000.33000.2 1.59 0.0669 80.3 2011.8322 2010.1343 7823.5 145 3.253 32.1 1 K.RKRLNRENTLEENT*R.A YNL091W 1 1 1.1% 1240 141514 5.4 U NST1 SGDID:S000005035, Chr XIV from 452410-456132, Verified ORF, "Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1" * Jamie_Alpha_SPB_16.05752.05752.2 2.0071 0.0411 87.6 1625.3121 1626.0391 6149.2 10 2.958 50.0 1 R.KLIQIAITKLLQSR.I Reverse_YAR042W 1 1 1.1% 1188 135070 5.6 U SWH1 SGDID:S000000081, Chr I from 192613-196179, Verified ORF, "Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction" * Jamie_Alpha_SPB_85.30447.30447.2 1.8024 0.2622 80.0 1464.2722 1466.5071 5877.5 122 4.858 37.5 1 R.SQY*AAIHLPTNGN.S YPL084W 1 1 1.1% 844 97276 5.4 U BRO1 SGDID:S000006005, Chr XVI from 394035-396569, Verified ORF, "Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes" * Jamie_Alpha_SPB_16.05515.05515.2 1.757 0.041 85.6 1130.3121 1131.2339 5805.7 99 3.316 56.2 1 R.RKAVENEER.E YLR106C 2 2 1.0% 4910 559316 5.0 U MDN1 SGDID:S000004096, Chr XII from 363739-349007, reverse complement, Verified ORF, "Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependent remodeling of 60S subunits and subsequent export from nucleoplasm to cytoplasm" * Jamie_Alpha_SPB_12.04135.04135.4 2.1885 0.3492 80.0 4506.6567 4507.0522 5433.7 124 4.969 9.5 1 P.ESLYLLS*SLRLLLNSLNALKLINEKST*HGKIDELTYIE.L * Jamie_Alpha_SPB_83.29834.29834.2 2.2153 0.2316 81.1 1577.1522 1577.6018 6056.2 135 4.518 41.7 1 K.NAKLTSFT*STIFS*.S YLR024C 1 1 1.0% 1872 216777 6.3 U UBR2 SGDID:S000004014, Chr XII from 193282-187664, reverse complement, Verified ORF, "Cytoplasmic ubiquitin-protein ligase (E3)" * Jamie_Alpha_SPB_56.20000.20000.2 1.3535 0.106 83.4 2096.912 2095.3135 7636.7 39 4.126 32.4 1 R.EQTTSYTPGILWPTKEDK.S YPL231W 1 1 1.0% 1887 206945 5.4 U FAS2 SGDID:S000006152, Chr XVI from 108652-114315, Verified ORF, "Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains beta-ketoacyl reductase and beta-ketoacyl synthase activities" * Jamie_Alpha_SPB_30.10815.10815.2 4.8329 0.4424 100.0 2290.3323 2290.4888 4095.8 1 6.972 63.9 1 L.KLEAEEIPSEDQNEFLLER.T Reverse_YHL035C 1 1 1.0% 1592 180925 8.5 U YHL035C SGDID:S000001027, Chr VIII from 32754-27976, reverse complement, Uncharacterized ORF, "Protein of unknown function, member of the ATP-binding cassette (ABC) family, potential Cdc28p substrate" * Jamie_Alpha_SPB_20.07178.07178.2 2.8533 0.0166 94.2 1895.0322 1895.1204 8374.1 29 3.812 43.3 1 R.RLTVLDIKS*IDQGDIK.I YGR109W-B 1 1 1.0% 1547 178419 8.8 U YGR109W-B SGDID:S000007347, Chr VII from 707614-708463,708465-712258, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YIL082W-A 1 1 1.0% 1498 172454 9.2 U YIL082W-A SGDID:S000003537, Chr IX from 205632-206482,206484-210129, transposable_element_gene, "Hypothetical protein" Jamie_Alpha_SPB_27.09552.09552.2 4.9241 0.5048 100.0 1729.7522 1729.8561 9930.2 1 8.069 78.6 1 K.DAMEEAYQTTALTER.F Reverse_YPL167C 1 1 1.0% 1504 172956 8.6 U REV3 SGDID:S000006088, Chr XVI from 237106-232592, reverse complement, Verified ORF, "Subunit of DNA polymerase zeta, which is involved in DNA repair; required for mutagenesis induced by DNA damage" * Jamie_Alpha_SPB_14.04858.04858.2 1.4309 0.0891 80.5 1842.2722 1843.9047 5284.8 119 3.038 32.1 1 R.QHRLT*STDTIQGDYK.I Reverse_YKL101W 1 1 1.0% 1518 169592 9.3 U HSL1 SGDID:S000001584, Chr XI from 248564-253120, Verified ORF, "Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p" * Jamie_Alpha_SPB_28.10040.10040.2 1.8754 0.0434 84.4 1828.9722 1831.0444 8028.1 71 2.913 35.7 1 R.RSRSSISTYSNRFIR.S Reverse_YIL126W 1 1 1.0% 1359 156742 6.7 U STH1 SGDID:S000001388, Chr IX from 117992-122071, Verified ORF, "ATPase component of the ATP-dependent RSC chromatin remodeling complex required for kinetochore function in chromosome segregation; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p" * Jamie_Alpha_SPB_82.29370.29370.2 1.655 0.1066 91.4 1553.5521 1550.7521 5092.3 16 3.366 45.8 1 K.DIKDFLIKEDASR.A YOR296W 1 1 1.0% 1289 147426 8.6 U YOR296W SGDID:S000005822, Chr XV from 870199-874068, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_51.18200.18200.2 1.4419 0.1182 89.6 1545.5122 1545.7968 6397.7 322 3.097 41.7 1 K.QPSFKVQDISHMK.Y Reverse_YOR227W 1 1 1.0% 1246 139457 8.9 U YOR227W SGDID:S000005753, Chr XV from 762825-766565, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_70.25075.25075.2 1.7633 0.0573 85.3 1631.1721 1632.6384 6898.0 26 3.46 45.8 1 K.S*QRTDIPPLETS*K.K Reverse_YER013W 1 1 1.0% 1145 130010 7.8 U PRP22 SGDID:S000000815, Chr V from 178840-182277, Verified ORF, "DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes" * Jamie_Alpha_SPB_21.07306.07306.2 2.0789 0.0106 83.3 1407.6721 1406.5382 7803.8 21 3.042 50.0 1 K.IRTDPGTVDEFR.I YAL019W 1 1 1.0% 1131 128507 5.4 U FUN30 SGDID:S000000017, Chr I from 114920-118315, Verified ORF, "Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p" * Jamie_Alpha_SPB_16.05539.05539.2 2.0544 0.0453 90.6 1515.5922 1515.49 5441.1 195 2.614 50.0 1 K.IEPYY*GS*LQER.E Reverse_YOR168W 1 1 1.0% 809 93133 8.4 U GLN4 SGDID:S000005694, Chr XV from 649303-651732, Verified ORF, "Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions" * Jamie_Alpha_SPB_12.04350.04350.2 1.8753 0.0428 89.4 904.7922 905.04156 3975.7 11 3.01 71.4 1 K.FSVTHSVK.I Reverse_YBR114W 1 1 1.0% 790 91430 8.3 U RAD16 SGDID:S000000318, Chr II from 467242-469614, Verified ORF, "Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4); member of the SWI/SNF family" * Jamie_Alpha_SPB_22.07653.07653.2 2.3091 0.2364 88.0 1081.2722 1080.0568 4357.9 15 4.368 71.4 1 Y.NVSKKT*S*R.T YBR156C 1 1 1.0% 698 79186 9.9 U SLI15 SGDID:S000000360, Chr II from 553194-551098, reverse complement, Verified ORF, "Subunit of the Ipl1p-Sli15p-Bir1p complex that regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; regulates the activity and localization of the Ipl1p aurora kinase" * Jamie_Alpha_SPB_15.05412.05412.2 1.8125 0.126 98.3 979.89215 978.94867 2990.1 9 3.685 75.0 1 K.VLRES*S*K.T Reverse_YBR275C 1 1 0.9% 1916 217959 6.6 U RIF1 SGDID:S000000479, Chr II from 757101-751351, reverse complement, Verified ORF, "Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation" * Jamie_Alpha_SPB_42.15159.15159.2 1.507 0.1314 91.7 2120.912 2121.4043 8488.8 32 3.783 34.4 1 K.GITKLT*ANYTRKDKFKR.N YML059C 1 1 0.9% 1679 187132 8.1 U NTE1 SGDID:S000004524, Chr XIII from 158258-153219, reverse complement, Verified ORF, "Serine esterase that deacylates exogenous lysophospholipids, homolog of human neuropathy target esterase (NTE); mammalian NTE1 deacylates phosphatidylcholine to glycerophosphocholine" * Jamie_Alpha_SPB_41.14790.14790.2 1.9615 0.0488 87.8 1735.1522 1734.0358 6918.7 162 3.207 32.1 1 R.MALVEAMLT*YLGVNK.S YJR031C 1 1 0.9% 1408 159437 5.0 U GEA1 SGDID:S000003792, Chr X from 490726-486500, reverse complement, Verified ORF, "Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea2p" * Jamie_Alpha_SPB_14.04914.04914.2 1.5754 0.105 90.0 1331.9922 1331.5956 5771.8 56 3.763 50.0 1 R.KTAEVTIAGITVK.L YMR129W 1 1 0.9% 1337 151652 6.7 U POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase;" * Jamie_Alpha_SPB_95.34218.34218.2 1.9576 0.2581 88.6 1508.1322 1505.6244 6105.0 2 3.916 50.0 1 K.YEFNGIPLKS*KE.R Reverse_YMR076C 1 1 0.9% 1277 147041 6.3 U PDS5 SGDID:S000004681, Chr XIII from 420028-416195, reverse complement, Verified ORF, "Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes in an interdependent manner, may function as a protein-protein interaction scaffold" * Jamie_Alpha_SPB_17.06019.06019.2 1.9903 0.0836 95.6 1489.3722 1489.5852 5219.8 39 3.55 50.0 1 K.KDFHS*LVT*LLR.H YBR059C 1 1 0.9% 1108 123989 7.7 U AKL1 SGDID:S000000263, Chr II from 360185-356859, reverse complement, Verified ORF, "Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization" * Jamie_Alpha_SPB_14.04906.04906.2 1.7969 0.0822 92.0 1149.2522 1148.9866 3771.6 304 3.962 55.6 1 K.STSS*YS*SGGR.S YBR073W 1 1 0.9% 924 104153 9.1 U RDH54 SGDID:S000000277, Chr II from 383209-385983, Verified ORF, "DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during mitosis and meiosis; proposed to be involved in crossover interference" * Jamie_Alpha_SPB_107.38570.38570.1 1.5187 0.2225 100.0 954.14 954.97876 5273.3 410 4.922 64.3 1 K.KVCNS*PGL.V Reverse_YLR452C 1 1 0.9% 698 79716 8.6 U SST2 SGDID:S000004444, Chr XII from 1041363-1039267, reverse complement, Verified ORF, "GTPase-activating protein for Gpa1p, regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family" * Jamie_Alpha_SPB_9.02918.02918.1 1.0616 0.0674 82.1 754.77 754.6885 5219.4 13 3.318 60.0 1 K.TDSS*HK.K YBR275C 1 1 0.8% 1916 217959 6.6 U RIF1 SGDID:S000000479, Chr II from 757101-751351, reverse complement, Verified ORF, "Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation" * Jamie_Alpha_SPB_24.08469.08469.2 1.8391 0.0449 83.5 1671.4922 1671.7178 6743.3 404 2.818 35.7 1 R.GFT*PGDLNGISVEER.R Reverse_YML103C 1 1 0.8% 1655 188576 5.6 U NUP188 SGDID:S000004571, Chr XIII from 67549-62582, reverse complement, Verified ORF, "Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with Pom152p and Nic96p" * Jamie_Alpha_SPB_78.27799.27799.2 2.0957 0.1382 98.3 1568.5122 1567.7367 7752.8 4 3.422 46.2 1 R.DGSSVLELLVLVS*R.L Reverse_YOR093C 1 1 0.8% 1648 186906 8.5 U YOR093C SGDID:S000005619, Chr XV from 502452-497506, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_88.31453.31453.2 1.6536 0.128 94.9 1742.8322 1739.8887 5638.3 181 3.586 38.5 1 K.SKRSNPSRLPT*YPR.M Reverse_YOR341W 1 1 0.8% 1664 186431 7.1 U RPA190 SGDID:S000005868, Chr XV from 960982-965976, Verified ORF, "RNA polymerase I subunit; largest subunit of RNA polymerase I" * Jamie_Alpha_SPB_20.07017.07017.2 1.8519 0.0399 83.0 1754.0721 1753.9126 4380.0 35 3.13 46.2 1 K.LIDT*RWKNGEATLR.L Reverse_YER166W 1 1 0.8% 1571 177797 6.0 U DNF1 SGDID:S000000968, Chr V from 512739-517454, Verified ORF, "Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport" * Jamie_Alpha_SPB_36.12920.12920.2 2.0158 0.252 80.0 1622.0322 1621.8695 7833.0 1 4.735 50.0 1 G.TFMVVGMAWKT*NR.L YPL058C 1 1 0.8% 1511 171064 6.8 U PDR12 SGDID:S000005979, Chr XVI from 450374-445839, reverse complement, Verified ORF, "Plasma membrane weak-acid-inducible ATP-binding cassette (ABC) transporter, required for weak organic acid resistance, strongly induced by sorbate and benzoate, regulated by War1p, mutants exhibit sorbate hypersensitivity" * Jamie_Alpha_SPB_50.17929.17929.2 1.6928 0.0813 89.3 1531.3322 1529.7794 4430.7 45 3.293 45.5 1 K.VWS*LKSVLNFKK.W Reverse_YMR190C 1 1 0.8% 1447 163837 6.3 U SGS1 SGDID:S000004802, Chr XIII from 645257-640914, reverse complement, Verified ORF, "Nucleolar DNA helicase of the RecQ family involved in maintenance of genome integrity, regulates chromosome synapsis and meiotic crossing over; has similarity to human BLM and WRN helicases implicated in Bloom and Werner syndromes" * Jamie_Alpha_SPB_33.11587.11587.2 1.6937 0.0481 82.2 1215.3121 1218.2664 4700.8 2 3.6 60.0 1 K.SSKSISRT*GSK.S Reverse_YDL145C 1 1 0.8% 1201 135607 6.0 U COP1 SGDID:S000002304, Chr IV from 198177-194572, reverse complement, Verified ORF, "Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway" * Jamie_Alpha_SPB_113.40677.40677.1 1.0432 0.2801 80.0 1203.14 1204.328 7557.5 10 4.379 44.4 1 R.WLKVQRDDSG.S Reverse_YBL076C 1 1 0.8% 1072 122983 6.1 U ILS1 SGDID:S000000172, Chr II from 84259-81041, reverse complement, Verified ORF, "Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A" * Jamie_Alpha_SPB_13.04593.04593.2 1.6753 0.0423 81.7 1323.9922 1324.3055 3594.0 1 2.961 81.2 1 K.Y*DNDFDIWR.G YKR086W 1 1 0.8% 1071 121652 8.2 U PRP16 SGDID:S000001794, Chr XI from 599499-602714, Verified ORF, "RNA helicase in the DEAH-box family involved in the second catalytic step of splicing, exhibits ATP-dependent RNA unwinding activity" * Jamie_Alpha_SPB_44.15867.15867.2 1.5924 0.118 95.0 1030.0521 1027.2552 3353.3 3 3.195 68.8 1 K.KIAPSINKR.K Reverse_YKL020C 1 1 0.8% 1082 121338 6.7 U SPT23 SGDID:S000001503, Chr XI from 401723-398475, reverse complement, Verified ORF, "ER membrane protein involved, with its homolog Mga2p, in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting" * Jamie_Alpha_SPB_15.05223.05223.2 1.5649 0.0559 80.7 1382.1322 1381.4259 5908.6 63 3.141 56.2 1 K.HHRCY*CVIR.T Reverse_YHR165C 1 1 0.7% 2413 279502 7.3 U PRP8 SGDID:S000001208, Chr VIII from 436950-429709, reverse complement, Verified ORF, "Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing" * Jamie_Alpha_SPB_27.09625.09625.2 2.3077 0.2313 82.9 2193.152 2193.548 4990.9 14 4.12 32.4 1 G.Y*IFAAVQIKVDSIEIFKK.L Reverse_YJR138W 1 1 0.7% 1584 181948 8.8 U IML1 SGDID:S000003899, Chr X from 684482-689236, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane" * Jamie_Alpha_SPB_45.15955.15955.2 1.9786 0.3231 90.5 1203.5721 1203.1644 5105.9 25 5.07 50.0 1 R.THSSGLYSSPS*.G YDR301W 1 1 0.7% 1357 153404 5.9 U CFT1 SGDID:S000002709, Chr IV from 1063346-1067419, Verified ORF, "RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF" * Jamie_Alpha_SPB_25.08709.08709.2 1.5145 0.1024 89.4 1189.8522 1189.308 6968.0 11 3.265 55.6 1 K.IESTT*IAFVK.E YDR104C 1 1 0.7% 1245 145181 9.1 U SPO71 SGDID:S000002511, Chr IV from 664905-661168, reverse complement, Verified ORF, "Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis" * Jamie_Alpha_SPB_31.10900.10900.2 1.7891 0.1194 96.9 1178.3322 1178.4197 5358.5 27 3.321 62.5 1 R.WKEYIVIAR.S Reverse_YAL063C 1 1 0.7% 1322 138072 4.3 U FLO9 SGDID:S000000059, Chr I from 27969-24001, reverse complement, Verified ORF, "Lectin-like protein with similarity to Flo1p, thought to be expressed and involved in flocculation" * Jamie_Alpha_SPB_26.09210.09210.2 1.6138 0.0707 87.5 1002.0722 1002.06665 4763.2 340 2.709 56.2 1 R.SISS*TVVTK.T Reverse_YLR096W 1 1 0.7% 1147 128338 9.4 U KIN2 SGDID:S000004086, Chr XII from 332591-336034, Verified ORF, "Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p" * Jamie_Alpha_SPB_22.07632.07632.2 1.6195 0.0478 83.2 1010.53217 1013.2747 5114.9 101 3.571 57.1 1 R.RQLKALKR.A YGR240C 1 1 0.7% 987 107970 6.4 U PFK1 SGDID:S000003472, Chr VII from 973739-970776, reverse complement, Verified ORF, "Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes" * Jamie_Alpha_SPB_13.04728.04728.1 2.2201 0.1198 83.3 867.18 866.9433 4116.4 15 3.778 75.0 1 R.DTEFIEL.Y YKL203C 1 1 0.6% 2474 281566 7.5 U TOR2 SGDID:S000001686, Chr XI from 63360-55936, reverse complement, Verified ORF, "PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis" * Jamie_Alpha_SPB_88.31640.31640.3 2.474 0.1009 82.1 1974.7743 1971.1802 3855.0 295 3.328 33.9 1 K.VLWLKSRSSET*WLER.R YER155C 1 1 0.6% 2167 245428 8.4 U BEM2 SGDID:S000000957, Chr V from 482843-476340, reverse complement, Verified ORF, "Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence" * Jamie_Alpha_SPB_16.05656.05656.2 1.868 0.1649 98.7 1622.2122 1622.7417 5325.7 251 3.254 42.3 1 K.RYVGAKSCS*VSISK.I YCR093W 1 1 0.6% 2108 240277 6.4 U CDC39 SGDID:S000000689, Chr III from 280113-286439, Verified ORF, "Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor" * Jamie_Alpha_SPB_65.23164.23164.2 1.814 0.0338 80.8 1501.6322 1501.4227 5667.7 88 2.589 40.9 1 K.DHS*QEGTTFNER.P Reverse_YPL085W 1 1 0.5% 2195 241694 5.4 U SEC16 SGDID:S000006006, Chr XVI from 387064-393651, Verified ORF, "COPII vesicle coat protein required for ER transport vesicle budding and autophagosome formation; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p" * Jamie_Alpha_SPB_47.16791.16791.2 1.686 0.0673 87.1 1562.6721 1561.6482 6410.0 182 3.972 40.9 1 K.EDYY*LTNKHGLK.A YGR032W 1 1 0.5% 1895 216988 7.0 U GSC2 SGDID:S000003264, Chr VII from 548268-553955, Verified ORF, "Catalytic subunit of 1,3-beta-glucan synthase, has similarity to an alternate catalytic subunit, Fks1p (Gsc1p); Rho1p encodes the regulatory subunit; involved in cell wall synthesis and maintenance" YLR342W 1 1 0.5% 1876 214849 7.2 U FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling" Jamie_Alpha_SPB_21.07453.07453.2 1.7423 0.0388 82.4 1193.3722 1193.493 4912.2 414 2.879 50.0 1 K.FLVSMQRLAK.F YOR093C 1 1 0.5% 1648 186906 8.5 U YOR093C SGDID:S000005619, Chr XV from 502452-497506, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_17.06014.06014.2 1.5167 0.0657 84.2 983.7322 984.011 4406.2 4 3.146 78.6 1 K.KENINS*AK.N YJL113W 1 1 0.4% 1803 207708 8.0 U YJL113W SGDID:S000003649, Chr X from 197834-198919,198921-203246, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" * Jamie_Alpha_SPB_15.05145.05145.1 1.4904 0.1059 94.4 946.67 947.03845 5267.6 26 3.751 66.7 1 K.YDEKVHR.I Reverse_YHR080C 1 1 0.4% 1345 149679 8.7 U YHR080C SGDID:S000001122, Chr VIII from 266840-262803, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Alpha_SPB_13.04460.04460.1 1.0423 0.0742 85.7 758.16 758.8094 4829.1 167 3.412 70.0 1 R.DKPENR.S YDR150W 1 1 0.3% 2748 313032 5.4 U NUM1 SGDID:S000002557, Chr IV from 755623-763869, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" * Jamie_Alpha_SPB_13.04651.04651.1 1.2003 0.0548 89.3 1022.02 1022.1864 4915.7 197 3.366 50.0 1 K.PTIDFLSTK.A Reverse_YKR054C 1 1 0.2% 4092 471351 6.3 U DYN1 SGDID:S000001762, Chr XI from 547567-535289, reverse complement, Verified ORF, "Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p" * Jamie_Alpha_SPB_7.02354.02354.2 1.6303 0.11 92.9 1125.6921 1125.2694 3854.0 109 3.6 50.0 1 R.LGDIFYGPSR.P Proteins Peptide IDs Spectra Unfiltered 1703 81080 86151 Filtered 262 650 721 Forward matches 158 539 610 Decoy matches 104 111 111 Forward FP rate 65.82 20.59 18.2 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0