DTASelect v2.0.26 /data/1/catclw/Projects/Jamie/Jamie_June2008/Ashy_SPB_blue/splitted /scratch/yates/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. --trypstat --cmax 4 --fp 0.9 true Use criteria 0.0 Minimum peptide confidence 0.9 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 2 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YIL002W-A 31 32 92.8% 69 7729 4.7 U YIL002W-A SGDID:S000028835, Chr IX from 350298-350507, Uncharacterized ORF, "Identified by expression profiling and mass spectrometry" * Jamie_Ashy_SPB_7.02400.02400.2 5.0695 0.3674 100.0 1447.5322 1448.5297 6364.6 1 8.028 84.6 2 M.TRDTPEDVSTAGAK.D * Jamie_Ashy_SPB_56.19986.19986.4 4.1336 0.3239 100.0 2584.5764 2585.9146 6741.9 1 5.517 33.3 1 M.TRDTPEDVSTAGAKDILDVLNLLK.G * Jamie_Ashy_SPB_56.19902.19902.3 7.2008 0.3752 100.0 2585.9644 2585.9146 8525.6 1 7.655 51.1 1 M.TRDTPEDVSTAGAKDILDVLNLLK.G * Jamie_Ashy_SPB_53.18826.18826.2 4.1252 0.421 100.0 3086.5723 3086.4233 6868.4 1 7.955 42.9 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEK.I * Jamie_Ashy_SPB_64.22810.22810.3 4.5258 0.2854 100.0 3757.5544 3757.184 9767.4 1 5.443 23.5 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEKISEVEL.K * Jamie_Ashy_SPB_59.21097.21097.4 7.2162 0.4872 100.0 3885.8965 3885.3582 10855.2 1 8.007 29.0 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEKISEVELK.L * Jamie_Ashy_SPB_66.23624.23624.3 5.7175 0.5377 100.0 4631.3945 4631.2036 8480.0 1 9.026 24.4 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEKISEVELKLDEMEK.K * Jamie_Ashy_SPB_66.23635.23635.4 6.7827 0.4277 100.0 4633.5366 4631.2036 11659.5 1 7.706 24.4 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEKISEVELKLDEMEK.K * Jamie_Ashy_SPB_63.22647.22647.3 6.4663 0.5472 100.0 4759.974 4759.378 8717.9 1 9.534 23.2 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEKISEVELKLDEMEKK.M * Jamie_Ashy_SPB_64.22884.22884.4 3.7577 0.1394 30.7 4763.4165 4759.378 7471.1 26 4.162 15.1 1 M.TRDTPEDVSTAGAKDILDVLNLLKGGEEKISEVELKLDEMEKK.M * Jamie_Ashy_SPB_6.01855.01855.2 3.0157 0.3381 100.0 1347.4722 1347.4246 6381.1 1 6.188 66.7 1 T.RDTPEDVSTAGAK.D * Jamie_Ashy_SPB_64.22994.22994.2 2.9677 0.3086 100.0 2327.9321 2328.622 9849.6 1 6.412 45.2 1 R.DTPEDVSTAGAKDILDVLNLLK.G * Jamie_Ashy_SPB_51.18132.18132.2 2.1827 0.1725 61.7 1412.7922 1412.7129 7070.8 1 4.889 66.7 1 A.GAKDILDVLNLLK.G * Jamie_Ashy_SPB_45.16164.16164.3 3.0505 0.2716 96.4 1914.5343 1913.2218 5437.1 1 4.478 36.8 1 A.GAKDILDVLNLLKGGEEK.I * Jamie_Ashy_SPB_68.24486.24486.3 5.3133 0.4274 100.0 3199.7644 3201.6973 9613.8 1 7.409 28.7 1 K.DILDVLNLLKGGEEKISEVELKLDEMEK.K * Jamie_Ashy_SPB_69.24534.24534.4 3.9868 0.2924 100.0 3200.9766 3201.6973 4996.6 1 6.033 27.2 1 K.DILDVLNLLKGGEEKISEVELKLDEMEK.K * Jamie_Ashy_SPB_66.23624.23624.2 1.9723 0.1036 20.3 3087.9321 3086.6086 8480.0 2 4.335 28.8 1 D.ILDVLNLLKGGEEKISEVELKLDEMEK.K * Jamie_Ashy_SPB_38.13675.13675.4 3.2185 0.0582 11.4 2532.3364 2532.9092 7567.4 16 4.292 26.2 1 L.NLLKGGEEKISEVELKLDEMEK.K * Jamie_Ashy_SPB_36.12769.12769.3 3.7449 0.2761 95.0 2660.1243 2661.0833 7172.8 33 5.618 27.3 1 L.NLLKGGEEKISEVELKLDEMEKK.M * Jamie_Ashy_SPB_38.13712.13712.3 4.085 0.2424 45.5 2768.1843 2767.1794 8519.5 1 5.093 35.9 1 L.KGGEEKISEVELKLDEMEKKMDSL.L * Jamie_Ashy_SPB_14.04976.04976.3 2.9365 0.0799 88.5 1319.4543 1318.4668 4468.4 264 3.193 38.6 1 K.GGEEKISEVELK.L * Jamie_Ashy_SPB_28.09841.09841.3 4.0345 0.1549 98.6 2064.7444 2064.3125 9498.3 1 5.198 41.2 1 K.GGEEKISEVELKLDEMEK.K * Jamie_Ashy_SPB_25.08772.08772.3 4.8864 0.344 100.0 2192.0044 2192.4866 9704.6 1 7.43 48.6 1 K.GGEEKISEVELKLDEMEKK.M * Jamie_Ashy_SPB_25.08689.08689.2 5.2802 0.4939 100.0 2192.7322 2192.4866 8059.6 1 8.675 63.9 1 K.GGEEKISEVELKLDEMEKK.M * Jamie_Ashy_SPB_42.14844.14844.4 6.063 0.3684 100.0 2638.6165 2639.0054 8727.7 1 7.397 31.1 1 K.GGEEKISEVELKLDEMEKKMDSL.L * Jamie_Ashy_SPB_55.19706.19706.4 6.2783 0.4619 100.0 4514.1367 4514.0767 7903.4 1 7.22 20.2 1 K.GGEEKISEVELKLDEMEKKMDSLLVQLEDLHRDNNDLAK.S * Jamie_Ashy_SPB_40.14304.14304.2 3.3032 0.2768 100.0 2139.4321 2138.4963 9473.7 3 4.909 41.2 1 K.ISEVELKLDEMEKKMDSL.L * Jamie_Ashy_SPB_55.19539.19539.3 5.1071 0.3405 100.0 4015.4343 4013.5679 10259.3 1 6.849 25.0 1 K.ISEVELKLDEMEKKMDSLLVQLEDLHRDNNDLAK.S * Jamie_Ashy_SPB_32.11400.11400.4 4.8012 0.2697 100.0 2469.8965 2468.7876 6853.5 1 5.734 35.8 1 K.KMDSLLVQLEDLHRDNNDLAK.S * Jamie_Ashy_SPB_36.12678.12678.4 3.2171 0.0468 79.9 2343.8564 2340.6135 4225.3 1 3.629 36.8 1 K.MDSLLVQLEDLHRDNNDLAK.S * Jamie_Ashy_SPB_36.12765.12765.2 3.6856 0.4149 100.0 2427.3323 2427.6917 7775.5 1 7.524 42.5 1 K.MDSLLVQLEDLHRDNNDLAKS.S YBR109C 12 13 85.0% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * Jamie_Ashy_SPB_28.09859.09859.2 3.9527 0.3263 100.0 1682.5322 1681.8151 8684.2 1 7.817 63.3 1 K.DNNGSISSSELATVMR.S * Jamie_Ashy_SPB_68.24268.24268.3 3.8119 0.4381 100.0 4108.1045 4109.526 7828.1 1 7.16 18.8 1 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * Jamie_Ashy_SPB_18.06492.06492.2 2.5355 0.2397 92.1 1603.4922 1604.7112 5703.9 12 4.6 57.7 1 R.QLKSNDSEQELLEA.F * Jamie_Ashy_SPB_36.12816.12816.2 2.4945 0.2096 83.0 1750.9122 1751.8877 9298.2 1 4.598 53.6 1 R.QLKSNDSEQELLEAF.K * Jamie_Ashy_SPB_34.12084.12084.2 3.1139 0.3245 100.0 1509.3121 1510.5975 8745.2 1 6.127 75.0 1 K.SNDSEQELLEAFK.V * Jamie_Ashy_SPB_34.12024.12024.1 2.6421 0.1438 100.0 1511.54 1510.5975 6462.1 1 4.318 54.2 2 K.SNDSEQELLEAFK.V * Jamie_Ashy_SPB_53.19063.19063.3 4.2221 0.2717 100.0 3169.4644 3169.4692 9991.3 1 4.946 32.1 1 K.SNDSEQELLEAFKVFDKNGDGLISAAELK.H * Jamie_Ashy_SPB_25.08971.08971.1 2.4469 0.3227 100.0 1189.54 1188.3231 6254.8 1 5.384 59.1 1 K.NGDGLISAAELK.H * Jamie_Ashy_SPB_53.18950.18950.3 4.4868 0.3813 100.0 3841.6743 3843.252 8410.5 1 6.677 25.0 1 A.ELKHVLTSIGEKLTDAEVDDMLREVSDGSGEINIQ.Q * Jamie_Ashy_SPB_62.22009.22009.3 4.1452 0.2418 95.8 3883.5842 3881.3008 8738.7 1 4.523 22.1 1 T.SIGEKLTDAEVDDMLREVSDGSGEINIQQFAALLSK.- * Jamie_Ashy_SPB_26.09160.09160.2 3.4138 0.2126 100.0 1280.2322 1278.4198 8043.7 1 4.973 85.0 1 K.LTDAEVDDMLR.E * Jamie_Ashy_SPB_51.18397.18397.2 3.9763 0.3928 100.0 2106.152 2107.325 10017.0 1 7.385 52.6 1 R.EVSDGSGEINIQQFAALLSK.- YIL149C 257 294 84.2% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_Ashy_SPB_55.19632.19632.2 3.9755 0.434 100.0 2409.2322 2409.7876 5498.9 1 7.751 42.5 1 K.ISEFLNVPFESLQGVTYPVLR.K * Jamie_Ashy_SPB_42.14888.14888.4 6.7275 0.4123 100.0 3377.8564 3375.8035 8512.9 1 6.918 26.5 1 K.IAKFERSEEEVTKLNVLVDEIKSQYYSR.I * Jamie_Ashy_SPB_34.12054.12054.2 4.5038 0.3755 100.0 1846.3522 1846.085 9346.6 1 7.018 70.0 1 R.SEEEVTKLNVLVDEIK.S * Jamie_Ashy_SPB_18.06427.06427.2 2.1928 0.1788 99.2 1746.4521 1746.9988 6635.0 50 4.169 39.3 1 R.ISKLKQLLDESSEQK.N * Jamie_Ashy_SPB_26.09150.09150.4 5.3681 0.4069 100.0 3827.9365 3830.2415 8553.1 2 6.662 21.4 1 R.ISKLKQLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Ashy_SPB_27.09726.09726.3 6.1068 0.3898 100.0 3501.2043 3501.8296 12353.4 1 8.411 31.9 3 K.LKQLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Ashy_SPB_20.07130.07130.4 4.4711 0.2323 100.0 3503.5366 3501.8296 8610.6 1 5.243 23.6 4 K.LKQLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Ashy_SPB_28.09862.09862.3 2.6711 0.0622 67.2 3744.8342 3745.0952 7833.8 17 3.394 20.2 1 K.LKQLLDESSEQKNTAKEELNGLKDQLNEERSR.Y * Jamie_Ashy_SPB_25.09001.09001.4 3.4795 0.0382 78.9 3745.1365 3745.0952 9663.8 20 4.061 18.8 1 K.LKQLLDESSEQKNTAKEELNGLKDQLNEERSR.Y * Jamie_Ashy_SPB_28.09889.09889.4 3.4068 0.2035 98.7 3260.2966 3260.496 7156.4 1 4.276 27.8 2 K.QLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Ashy_SPB_28.09804.09804.3 2.5465 0.044 54.0 3263.3643 3260.496 5249.4 3 3.073 23.1 1 K.QLLDESSEQKNTAKEELNGLKDQLNEER.S * Jamie_Ashy_SPB_19.06570.06570.4 3.4529 0.1129 91.2 3505.0166 3503.7617 7848.3 1 4.032 25.3 1 K.QLLDESSEQKNTAKEELNGLKDQLNEERSR.Y * Jamie_Ashy_SPB_20.07243.07243.2 3.7562 0.4253 100.0 2100.4922 2102.2656 6921.7 1 7.563 52.9 1 K.NTAKEELNGLKDQLNEER.S * Jamie_Ashy_SPB_27.09624.09624.3 1.9216 0.0035 33.5 2101.8843 2102.2656 5585.2 120 2.882 29.4 1 K.NTAKEELNGLKDQLNEER.S * Jamie_Ashy_SPB_11.03930.03930.2 2.3464 0.2401 100.0 1292.1721 1292.524 4212.8 2 4.857 77.8 1 R.YRREIDALKK.Q * Jamie_Ashy_SPB_18.06313.06313.4 3.9673 0.2395 99.2 2877.4966 2877.248 7006.0 1 4.812 27.5 1 R.EIDALKKQLHVSHEAMREVNDEKR.V * Jamie_Ashy_SPB_5.01836.01836.2 2.0209 0.2934 100.0 1208.0922 1208.3806 1745.7 1 6.085 88.9 1 K.QLHVSHEAMR.E * Jamie_Ashy_SPB_24.08616.08616.3 2.0245 0.0425 38.3 3512.6343 3513.8694 6894.9 108 3.442 18.5 1 K.QLHVSHEAMREVNDEKRVKEEYDIWQSR.D * Jamie_Ashy_SPB_1.00374.00374.2 3.1371 0.0633 61.4 1602.3121 1601.7344 5202.2 1 4.08 66.7 1 V.SHEAMREVNDEKR.V * Jamie_Ashy_SPB_23.08220.08220.4 4.2995 0.1683 98.6 4122.0566 4125.325 7409.5 49 4.204 17.2 1 R.EVNDEKRVKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Ashy_SPB_29.10224.10224.4 3.8347 0.1207 93.7 4506.8564 4507.8315 8886.5 212 4.546 16.7 1 R.EVNDEKRVKEEYDIWQSRDQGNDSLNDDLNKENKLLR.R * Jamie_Ashy_SPB_19.06885.06885.4 5.7482 0.3418 100.0 3411.7766 3410.595 8210.5 1 5.429 25.9 1 K.RVKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Ashy_SPB_19.06746.06746.1 2.3541 0.3452 100.0 1452.66 1453.5944 4737.7 1 6.345 60.0 1 R.VKEEYDIWQSR.D * Jamie_Ashy_SPB_21.07578.07578.2 2.9059 0.2627 100.0 3253.7522 3254.4075 6565.0 1 6.389 30.8 1 R.VKEEYDIWQSRDQGNDSLNDDLNKENK.L * Jamie_Ashy_SPB_19.06774.06774.2 2.1265 0.0921 94.1 2201.9922 2202.3428 5844.1 4 3.782 41.7 1 R.DQGNDSLNDDLNKENKLLR.R * Jamie_Ashy_SPB_30.10602.10602.2 1.7911 0.0229 75.3 1277.0322 1277.5397 5888.1 21 3.828 66.7 1 K.LMEMENILQR.C * Jamie_Ashy_SPB_33.11610.11610.2 3.2666 0.3552 100.0 2015.9521 2016.2324 8683.2 1 6.343 46.7 1 K.LYQMQSNYESVFTYNK.F * Jamie_Ashy_SPB_35.12301.12301.4 2.9929 0.1428 92.5 2506.2166 2506.9202 5671.5 45 3.756 27.5 1 K.FLLNQNKQLSQSVEEKVLEMK.N * Jamie_Ashy_SPB_35.12291.12291.3 4.3703 0.3357 100.0 2506.8245 2506.9202 7451.8 1 5.971 35.0 1 K.FLLNQNKQLSQSVEEKVLEMK.N * Jamie_Ashy_SPB_5.01539.01539.1 2.0954 0.052 93.9 1105.63 1105.2334 3985.5 13 3.187 61.1 1 K.NLKDTASVEK.A * Jamie_Ashy_SPB_11.03894.03894.2 3.2331 0.351 100.0 1177.2922 1176.3121 4578.2 1 5.913 80.0 1 K.NLKDTASVEKA.E * Jamie_Ashy_SPB_22.07848.07848.3 5.0358 0.4759 100.0 2396.9944 2398.734 7002.8 1 7.895 43.8 1 K.NLKDTASVEKAEFSKEMTLQK.N * Jamie_Ashy_SPB_13.04671.04671.1 2.0688 0.0496 93.8 906.52 906.02386 5302.3 3 3.531 71.4 1 R.SQLTSLEK.D * Jamie_Ashy_SPB_17.05854.05854.1 2.2922 0.3006 100.0 1536.56 1537.6764 3819.1 2 5.898 50.0 1 R.SQLTSLEKDCSLR.A * Jamie_Ashy_SPB_16.05804.05804.2 3.8817 0.3079 100.0 1539.4122 1537.6764 6365.7 1 5.824 62.5 1 R.SQLTSLEKDCSLR.A * Jamie_Ashy_SPB_27.09709.09709.4 3.8039 0.0247 80.2 3047.4966 3043.1938 8266.1 2 3.408 24.7 1 R.AIEKNDDNSCRNPEHTDVIDELIDTK.L * Jamie_Ashy_SPB_33.11767.11767.4 3.9961 0.1156 94.2 3684.9365 3682.9897 7070.9 48 3.363 18.9 1 R.AIEKNDDNSCRNPEHTDVIDELIDTKLRLEK.S * Jamie_Ashy_SPB_29.10332.10332.3 5.2202 0.373 100.0 2603.6042 2601.666 8222.5 1 7.251 40.5 1 K.NDDNSCRNPEHTDVIDELIDTK.L * Jamie_Ashy_SPB_32.11280.11280.2 4.4084 0.474 100.0 1738.8522 1739.8767 8541.2 1 8.255 82.1 2 R.NPEHTDVIDELIDTK.L * Jamie_Ashy_SPB_4.01206.01206.1 1.8949 0.2408 100.0 846.48 845.9713 4970.6 1 4.757 68.8 1 T.SAVSPTVGK.L * Jamie_Ashy_SPB_61.22002.22002.3 5.1491 0.4296 100.0 3067.9443 3069.4846 8556.2 1 7.326 32.3 2 R.NQKFQLQNQLEDFILELEHKTPELI.S * Jamie_Ashy_SPB_61.21956.21956.2 2.7243 0.174 80.0 3068.652 3069.4846 9093.0 1 5.973 31.2 1 R.NQKFQLQNQLEDFILELEHKTPELI.S * Jamie_Ashy_SPB_60.21444.21444.3 5.7713 0.4506 100.0 3432.2344 3431.9133 8644.4 1 7.958 25.0 1 R.NQKFQLQNQLEDFILELEHKTPELISFK.E * Jamie_Ashy_SPB_56.20174.20174.3 6.2599 0.5371 100.0 3715.9443 3717.2163 8725.1 1 8.816 33.6 1 R.NQKFQLQNQLEDFILELEHKTPELISFKER.T * Jamie_Ashy_SPB_56.20154.20154.4 6.5284 0.3997 100.0 3718.5366 3717.2163 7863.1 1 6.995 28.2 1 R.NQKFQLQNQLEDFILELEHKTPELISFKER.T * Jamie_Ashy_SPB_59.21013.21013.2 4.5319 0.5054 100.0 2144.392 2145.4197 8464.7 1 8.632 65.6 2 K.FQLQNQLEDFILELEHK.T * Jamie_Ashy_SPB_62.22110.22110.2 4.3144 0.3984 100.0 2181.912 2182.4785 9674.0 1 7.09 55.9 1 Q.NQLEDFILELEHKTPELI.S * Jamie_Ashy_SPB_56.20174.20174.2 2.0498 0.0878 17.0 2477.632 2474.8162 8725.1 51 3.269 26.3 1 L.EDFILELEHKTPELISFKER.T * Jamie_Ashy_SPB_4.01158.01158.2 2.5299 0.1269 100.0 1085.2522 1085.2456 4984.3 2 4.149 75.0 1 R.TKSLEHELK.R * Jamie_Ashy_SPB_5.01722.01722.2 2.9954 0.3692 100.0 1242.0922 1241.4331 5632.2 1 6.619 88.9 3 R.TKSLEHELKR.S * Jamie_Ashy_SPB_7.02281.02281.2 2.1592 0.1154 98.5 1013.27216 1012.1539 7131.4 5 4.207 78.6 3 K.SLEHELKR.S * Jamie_Ashy_SPB_21.07340.07340.2 2.7472 0.1079 99.2 1477.0721 1477.6982 5225.8 2 4.503 66.7 1 K.RSTELLETVSLTK.R * Jamie_Ashy_SPB_8.02874.02874.2 2.4286 0.2112 100.0 1260.1921 1260.4362 5229.2 1 5.164 83.3 1 R.KQEREITSLR.Q * Jamie_Ashy_SPB_15.05372.05372.2 3.3389 0.3265 100.0 1454.8522 1455.6201 4883.0 1 5.878 70.8 1 K.INGCEANIHSLVK.Q * Jamie_Ashy_SPB_23.08125.08125.3 3.3214 0.2093 98.7 2374.4944 2374.6104 7940.3 1 4.791 30.0 1 R.KILESSNIVNENDSQAIITER.L * Jamie_Ashy_SPB_24.08421.08421.1 2.2325 0.122 100.0 1550.09 1549.721 5557.9 1 4.492 50.0 1 R.LVEFSNVNELQEK.N * Jamie_Ashy_SPB_41.14508.14508.2 4.9193 0.3296 100.0 2661.9521 2661.9812 9465.3 1 7.052 52.4 1 R.LVEFSNVNELQEKNVELLNCIR.I * Jamie_Ashy_SPB_41.14480.14480.3 4.1872 0.2398 100.0 2664.4443 2661.9812 6241.5 1 5.691 39.3 1 R.LVEFSNVNELQEKNVELLNCIR.I * Jamie_Ashy_SPB_14.05046.05046.3 2.5725 0.0502 69.8 1766.0343 1764.9731 7133.1 7 4.17 39.3 1 R.ILADKLENYEGKQDK.T * Jamie_Ashy_SPB_15.05361.05361.2 3.2627 0.1134 100.0 2236.632 2235.5425 7258.5 2 4.992 38.9 1 R.ILADKLENYEGKQDKTLQK.V * Jamie_Ashy_SPB_52.18427.18427.4 4.5734 0.0725 93.8 4705.4565 4701.3286 10121.0 2 3.451 18.3 1 R.ILADKLENYEGKQDKTLQKVENQTIKEAKDAIIELENINAK.M * Jamie_Ashy_SPB_7.02238.02238.3 3.3926 0.1692 62.5 1538.5743 1538.6543 8767.9 1 4.552 47.9 1 L.ADKLENYEGKQDK.T * Jamie_Ashy_SPB_47.16702.16702.2 2.8858 0.0975 99.2 2955.4521 2955.3787 7651.9 1 4.419 36.0 1 K.TLQKVENQTIKEAKDAIIELENINAK.M * Jamie_Ashy_SPB_32.11364.11364.2 2.2857 0.0968 96.1 2188.7322 2189.4888 5675.6 18 4.082 30.6 1 K.EAKDAIIELENINAKMETR.I * Jamie_Ashy_SPB_29.10444.10444.1 2.8622 0.1818 100.0 1342.26 1343.5199 5821.9 1 5.048 63.6 1 K.DAIIELENINAK.M * Jamie_Ashy_SPB_34.12066.12066.2 3.7011 0.5139 100.0 1859.5922 1861.1205 9286.0 1 8.944 73.3 1 K.DAIIELENINAKMETR.I * Jamie_Ashy_SPB_22.07933.07933.3 3.0581 0.1136 88.1 2832.5044 2832.0686 8607.1 2 3.813 25.0 1 R.DSYKLLASTEENKANTNSVTSMEAAR.E * Jamie_Ashy_SPB_22.07646.07646.2 5.1821 0.456 100.0 2832.612 2832.0686 5687.7 1 8.735 52.0 1 R.DSYKLLASTEENKANTNSVTSMEAAR.E * Jamie_Ashy_SPB_19.06801.06801.3 4.2574 0.4592 100.0 3086.6343 3089.3582 9143.0 1 7.076 25.9 1 R.DSYKLLASTEENKANTNSVTSMEAAREK.K * Jamie_Ashy_SPB_19.06786.06786.4 4.5057 0.1505 98.6 3092.6565 3089.3582 8182.1 2 4.918 24.7 1 R.DSYKLLASTEENKANTNSVTSMEAAREK.K * Jamie_Ashy_SPB_16.05578.05578.2 2.4883 0.3063 100.0 2337.9922 2338.5518 6584.7 1 6.042 33.3 1 K.LLASTEENKANTNSVTSMEAAR.E * Jamie_Ashy_SPB_30.10651.10651.3 3.096 0.1971 96.0 3127.7644 3128.5967 6825.0 453 4.116 19.2 1 R.EKKIRELEAELSSTKVENSAIIQNLRK.E * Jamie_Ashy_SPB_16.05569.05569.2 2.1111 0.1745 61.2 1188.6122 1188.3666 4687.1 16 3.847 61.1 1 K.KIRELEAELS.S * Jamie_Ashy_SPB_31.10954.10954.3 3.1786 0.1208 89.8 2740.5544 2743.133 7385.7 17 3.887 23.9 1 K.KIRELEAELSSTKVENSAIIQNLR.K * Jamie_Ashy_SPB_18.06457.06457.2 2.4967 0.1743 72.7 1807.4722 1805.9799 7889.8 1 4.182 53.3 1 K.IRELEAELSSTKVENS.A * Jamie_Ashy_SPB_14.04992.04992.1 2.8942 0.3159 100.0 1107.47 1107.2029 2812.4 1 5.972 72.2 1 R.ELEAELSSTK.V * Jamie_Ashy_SPB_13.04435.04435.2 2.8607 0.135 100.0 1409.9122 1410.663 4084.9 3 4.026 80.0 1 K.ELLIYKKSQCK.K * Jamie_Ashy_SPB_21.07267.07267.4 3.353 0.1964 98.8 3047.1765 3044.4443 5902.6 29 4.259 21.5 1 K.KSQCKKKTTLEDFENFKGLAKEKER.M * Jamie_Ashy_SPB_19.06579.06579.2 1.6372 0.0111 63.8 2413.5322 2412.7483 7307.3 38 2.845 31.6 1 K.KKTTLEDFENFKGLAKEKER.M * Jamie_Ashy_SPB_26.09309.09309.2 2.3941 0.0103 85.6 1743.5922 1741.9817 7839.8 65 2.937 42.9 1 K.KTTLEDFENFKGLAK.E * Jamie_Ashy_SPB_21.07266.07266.4 3.4008 0.1963 98.6 2284.7766 2284.5742 6527.5 6 4.513 32.4 1 K.KTTLEDFENFKGLAKEKER.M * Jamie_Ashy_SPB_21.07252.07252.2 2.7205 0.0689 97.1 2285.2522 2284.5742 6777.6 1 3.573 44.4 1 K.KTTLEDFENFKGLAKEKER.M * Jamie_Ashy_SPB_21.07267.07267.3 4.684 0.3376 100.0 2285.6343 2284.5742 5902.6 1 6.56 43.1 1 K.KTTLEDFENFKGLAKEKER.M * Jamie_Ashy_SPB_30.10586.10586.1 2.404 0.2329 100.0 1244.41 1244.3434 5419.4 2 5.295 66.7 1 K.TTLEDFENFK.G * Jamie_Ashy_SPB_24.08640.08640.4 2.5957 0.2096 94.1 2156.3765 2156.4001 3777.8 2 3.899 39.2 1 K.TTLEDFENFKGLAKEKER.M * Jamie_Ashy_SPB_25.08724.08724.3 3.4149 0.1809 98.7 2157.3843 2156.4001 5108.2 5 5.075 30.9 1 K.TTLEDFENFKGLAKEKER.M * Jamie_Ashy_SPB_28.10033.10033.2 3.2395 0.3332 100.0 1512.3722 1512.7026 7926.9 1 6.026 70.8 1 T.TLEDFENFKGLAK.E * Jamie_Ashy_SPB_21.07532.07532.2 2.4394 0.2885 97.0 2055.6921 2055.2952 8270.8 1 5.309 40.6 1 T.TLEDFENFKGLAKEKER.M * Jamie_Ashy_SPB_38.13476.13476.2 4.6216 0.4083 100.0 1770.1522 1770.0509 9227.1 1 8.009 71.4 1 R.MLEEAIDHLKAELEK.Q * Jamie_Ashy_SPB_35.12534.12534.4 2.8642 0.0827 82.1 2025.0165 2026.3557 4185.3 3 5.05 36.5 1 R.MLEEAIDHLKAELEKQK.S * Jamie_Ashy_SPB_7.02538.02538.2 2.0351 0.0966 29.1 1082.4321 1083.2297 2997.6 21 3.617 62.5 1 I.DHLKAELEK.Q * Jamie_Ashy_SPB_8.02566.02566.1 2.5471 0.2211 100.0 1083.16 1083.2297 4771.7 5 5.189 62.5 1 I.DHLKAELEK.Q * Jamie_Ashy_SPB_8.02892.02892.2 3.4047 0.2316 100.0 1340.3322 1339.5345 4397.8 1 4.831 80.0 2 I.DHLKAELEKQK.S * Jamie_Ashy_SPB_20.06951.06951.2 3.5785 0.4102 100.0 1631.7322 1630.8436 4603.0 1 7.733 75.0 1 K.SWVPSYIHVEKER.A * Jamie_Ashy_SPB_3.00817.00817.2 3.1039 0.2913 100.0 1264.3121 1264.3378 3468.8 1 6.452 75.0 1 K.ERASTELSQSR.I * Jamie_Ashy_SPB_18.06246.06246.2 3.6237 0.2737 100.0 1451.1322 1451.7466 4890.6 3 5.518 68.2 1 R.IKIKSLEYEISK.L * Jamie_Ashy_SPB_3.00883.00883.2 3.0441 0.1905 100.0 1428.5322 1428.5419 6614.7 1 4.88 72.7 1 R.LKESEISHNENK.M * Jamie_Ashy_SPB_13.04650.04650.3 3.7605 0.1183 95.5 2124.0244 2124.33 5299.5 2 3.935 41.2 1 R.LKESEISHNENKMDFSSK.E * Jamie_Ashy_SPB_14.04773.04773.3 5.7682 0.4266 100.0 2729.6042 2729.9783 8648.5 1 7.493 40.9 1 R.LKESEISHNENKMDFSSKEGQYK.A * Jamie_Ashy_SPB_10.03493.03493.1 1.5492 0.0691 88.6 714.56 714.8114 4186.1 14 4.534 70.0 1 K.MDFSSK.E * Jamie_Ashy_SPB_13.04674.04674.1 2.6766 0.1028 100.0 1258.47 1258.4172 3845.8 1 4.572 66.7 1 K.IKELENNLER.L * Jamie_Ashy_SPB_16.05749.05749.2 2.3695 0.0217 90.0 1527.1122 1527.7642 5839.1 11 4.192 59.1 1 K.IKELENNLERLR.S * Jamie_Ashy_SPB_13.04488.04488.2 1.8591 0.0018 77.7 1016.97217 1017.0837 5925.6 7 3.283 85.7 1 K.ELENNLER.L * Jamie_Ashy_SPB_32.11466.11466.2 3.2826 0.3081 100.0 1648.0521 1646.8381 9030.0 1 6.249 69.2 1 R.SDLQSKIQEIESIR.S * Jamie_Ashy_SPB_23.08035.08035.2 3.744 0.2654 100.0 2400.7522 2399.6008 9386.0 1 6.113 52.6 1 R.SCKDSQLKWAQNTIDDTEMK.M * Jamie_Ashy_SPB_17.06060.06060.1 2.4412 0.2858 100.0 1451.36 1452.5787 5278.0 1 5.607 63.6 1 K.WAQNTIDDTEMK.M * Jamie_Ashy_SPB_52.18457.18457.3 3.5189 0.1237 93.7 3494.4543 3493.9922 7362.7 1 4.275 26.7 1 K.MKSLLTELSNKETTIEKLSSEIENLDKELR.K * Jamie_Ashy_SPB_27.09480.09480.2 3.9217 0.2854 100.0 1706.5721 1706.931 9632.5 1 6.837 67.9 1 K.SLLTELSNKETTIEK.L * Jamie_Ashy_SPB_55.19594.19594.2 4.0195 0.4449 100.0 3233.6921 3234.6257 6549.2 1 8.275 37.0 1 K.SLLTELSNKETTIEKLSSEIENLDKELR.K * Jamie_Ashy_SPB_51.18384.18384.4 6.7279 0.306 100.0 3362.3364 3362.7998 8136.1 1 7.724 32.1 1 K.SLLTELSNKETTIEKLSSEIENLDKELRK.T * Jamie_Ashy_SPB_52.18652.18652.3 5.491 0.3659 100.0 3465.8342 3463.9048 7789.1 1 6.747 26.7 1 K.SLLTELSNKETTIEKLSSEIENLDKELRKT.K * Jamie_Ashy_SPB_16.05766.05766.2 2.7375 0.2542 100.0 1147.4321 1148.2554 5303.5 1 5.626 88.9 1 K.LSSEIENLDK.E * Jamie_Ashy_SPB_18.06240.06240.3 2.6329 0.1745 23.5 1562.0944 1561.7324 4626.2 1 3.753 56.2 1 L.SSEIENLDKELRK.T * Jamie_Ashy_SPB_25.08733.08733.3 3.9113 0.2017 98.6 2731.7043 2732.0654 6248.2 1 4.341 33.0 1 K.TKFQYKFLDQNSDASTLEPTLRK.E * Jamie_Ashy_SPB_28.09888.09888.3 4.1052 0.3552 100.0 2502.0544 2502.7864 8777.6 1 6.459 42.5 1 K.FQYKFLDQNSDASTLEPTLRK.E * Jamie_Ashy_SPB_23.08034.08034.2 2.0674 0.1381 30.8 1934.8722 1936.1289 7230.8 20 4.019 37.5 1 Y.KFLDQNSDASTLEPTLR.K * Jamie_Ashy_SPB_72.25905.25905.4 5.0194 0.2945 100.0 6378.4966 6376.07 7458.8 1 4.809 14.5 1 K.FLDQNSDASTLEPTLRKELEQIQVQLKDANSQIQAYEEIISSNENALIELKNELAK.T * Jamie_Ashy_SPB_64.22868.22868.2 2.9296 0.3319 100.0 3247.912 3249.556 8582.0 1 7.286 32.1 1 K.DANSQIQAYEEIISSNENALIELKNELAK.T * Jamie_Ashy_SPB_64.22788.22788.3 3.9089 0.2737 100.0 3251.7244 3249.556 7813.4 1 4.839 27.7 1 K.DANSQIQAYEEIISSNENALIELKNELAK.T * Jamie_Ashy_SPB_17.06132.06132.4 2.7582 0.1311 18.1 4145.3364 4149.691 8659.4 25 3.265 15.7 1 L.IELKNELAKTKENYDAKIELEKKEKWAREEDLSR.L * Jamie_Ashy_SPB_13.04422.04422.4 3.5391 0.2086 99.2 1708.3765 1709.9371 5099.0 16 4.993 34.6 1 K.TKENYDAKIELEKK.E * Jamie_Ashy_SPB_13.04522.04522.2 2.9002 0.0733 97.7 1711.8121 1709.9371 6384.6 1 3.65 76.9 2 K.TKENYDAKIELEKK.E * Jamie_Ashy_SPB_118.42520.42520.3 2.5195 0.098 79.7 1966.9744 1967.2267 8035.5 22 3.45 35.0 1 K.TKENYDAKIELEKKEK.W * Jamie_Ashy_SPB_13.04696.04696.2 2.808 0.214 100.0 1967.6322 1967.2267 5749.3 1 4.965 53.3 1 K.TKENYDAKIELEKKEK.W * Jamie_Ashy_SPB_14.04872.04872.3 2.588 0.2114 94.1 2382.9243 2380.7063 7778.8 8 4.167 30.6 1 K.TKENYDAKIELEKKEKWAR.E * Jamie_Ashy_SPB_18.06235.06235.4 4.1266 0.2521 95.2 2957.0166 2954.2634 7055.0 1 4.977 26.8 1 K.TKENYDAKIELEKKEKWAREEDLS.R * Jamie_Ashy_SPB_17.06132.06132.3 3.7516 0.3305 100.0 3109.2544 3110.451 8659.4 1 6.045 31.2 1 K.TKENYDAKIELEKKEKWAREEDLSR.L * Jamie_Ashy_SPB_13.04532.04532.1 1.5927 0.0406 85.5 1418.81 1419.537 2971.3 21 3.808 55.0 1 K.EKWAREEDLSR.L * Jamie_Ashy_SPB_18.06452.06452.2 2.1773 0.0819 94.0 1581.3722 1580.871 5889.5 1 3.372 53.8 1 R.LRGELGEIRALQPK.L * Jamie_Ashy_SPB_22.07884.07884.3 4.9296 0.4396 100.0 2510.3342 2511.8435 6769.8 1 6.699 37.5 1 K.LKEGALHFVQQSEKLRNEVER.I * Jamie_Ashy_SPB_22.07890.07890.2 2.9702 0.289 100.0 2511.2322 2511.8435 5731.6 4 5.017 37.5 1 K.LKEGALHFVQQSEKLRNEVER.I * Jamie_Ashy_SPB_22.07857.07857.4 2.3941 0.0845 67.7 2516.6165 2511.8435 3287.2 25 3.272 26.7 1 K.LKEGALHFVQQSEKLRNEVER.I * Jamie_Ashy_SPB_27.09572.09572.3 2.717 0.0087 48.3 2881.6743 2881.3079 7902.0 120 3.232 22.8 1 K.LKEGALHFVQQSEKLRNEVERIQK.M * Jamie_Ashy_SPB_27.09741.09741.4 3.2437 0.0713 82.0 2884.4165 2881.3079 4991.7 17 3.486 22.5 1 K.LKEGALHFVQQSEKLRNEVERIQK.M * Jamie_Ashy_SPB_22.07746.07746.3 2.401 0.1873 90.0 2269.7944 2270.51 5622.4 1 4.259 30.6 1 K.EGALHFVQQSEKLRNEVER.I * Jamie_Ashy_SPB_2.00762.00762.2 2.1762 0.0415 94.2 1016.8122 1016.1423 3819.2 97 2.965 71.4 1 R.NEVERIQK.M * Jamie_Ashy_SPB_6.02154.02154.2 2.4381 2.0E-4 95.1 892.4322 891.1138 4317.7 19 2.675 100.0 1 K.MIEKIEK.M * Jamie_Ashy_SPB_18.06349.06349.2 2.5444 0.2527 100.0 1081.5322 1080.2941 5472.3 18 4.87 75.0 1 K.MSTIVQLCK.K * Jamie_Ashy_SPB_45.16147.16147.3 4.6648 0.3025 100.0 3020.7544 3021.376 8016.6 1 6.116 27.0 1 T.MKENKDLSELVIRLEKDAADCQAELT.K * Jamie_Ashy_SPB_57.20502.20502.2 2.2957 0.1192 33.6 2391.5322 2390.616 10421.0 2 3.661 32.5 1 K.DLSELVIRLEKDAADCQAELT.K * Jamie_Ashy_SPB_13.04494.04494.3 3.2082 0.328 100.0 1595.0643 1592.7095 6111.7 1 5.563 51.9 1 R.LEKDAADCQAELTK.T * Jamie_Ashy_SPB_18.06190.06190.1 1.2501 0.1057 86.7 1222.25 1222.2604 5836.7 55 3.786 45.0 1 K.DAADCQAELTK.T * Jamie_Ashy_SPB_25.08838.08838.2 2.2098 0.1305 97.7 1223.3922 1222.2604 6135.2 12 4.5 65.0 3 K.DAADCQAELTK.T * Jamie_Ashy_SPB_16.05630.05630.3 4.0846 0.4276 100.0 1994.8744 1992.196 4151.0 1 7.128 45.3 1 K.TKSSLYSAQDLLDKHER.K * Jamie_Ashy_SPB_16.05623.05623.4 3.3986 0.2066 81.6 1893.8165 1891.0911 7048.7 1 4.457 40.0 1 T.KSSLYSAQDLLDKHER.K * Jamie_Ashy_SPB_19.06664.06664.4 2.4828 0.1281 87.4 1763.0165 1762.917 4662.0 4 3.684 31.0 1 K.SSLYSAQDLLDKHER.K * Jamie_Ashy_SPB_23.08205.08205.2 2.1678 0.1235 28.2 1841.4122 1841.0887 6773.2 7 3.365 46.2 1 R.KWMEEKADYERELI.S * Jamie_Ashy_SPB_27.09692.09692.2 2.6089 0.2043 82.5 1711.6921 1712.9147 6599.4 1 4.336 58.3 1 K.WMEEKADYERELI.S * Jamie_Ashy_SPB_1.00379.00379.2 3.6239 0.3299 100.0 1612.7922 1613.6812 5828.1 1 6.503 57.1 2 K.VDDTAANNGDKDHLK.L * Jamie_Ashy_SPB_20.06980.06980.2 2.9137 0.3217 100.0 1470.8722 1471.7024 4967.2 3 5.325 59.1 1 K.LVSLFSNLRHER.N * Jamie_Ashy_SPB_29.10423.10423.3 4.5623 0.1215 98.7 2491.4343 2490.819 7225.5 1 5.58 40.0 1 R.ELAFVKQKNDSLEKTINDLQR.T * Jamie_Ashy_SPB_17.06156.06156.2 2.3174 0.1815 99.6 1802.4122 1802.9823 3897.8 108 5.123 42.9 1 K.QKNDSLEKTINDLQR.T * Jamie_Ashy_SPB_43.15309.15309.3 3.5311 0.1321 93.9 2846.9343 2848.1426 6943.7 1 4.233 28.3 1 R.TQTLSEKEYQCSAVIIDEFKDITK.E * Jamie_Ashy_SPB_58.20599.20599.3 5.6155 0.3415 100.0 3405.1143 3404.759 8638.1 1 6.317 27.7 1 R.TQTLSEKEYQCSAVIIDEFKDITKEVTQV.N * Jamie_Ashy_SPB_32.11244.11244.2 3.4108 0.2428 100.0 1604.6721 1602.7473 9035.2 3 4.933 54.2 1 K.EYQCSAVIIDEFK.D * Jamie_Ashy_SPB_1.00385.00385.1 2.0789 0.0775 96.4 918.52 919.066 3332.4 2 4.414 64.3 1 K.SLKNVTEK.N * Jamie_Ashy_SPB_18.06414.06414.3 4.1669 0.392 100.0 3091.3442 3092.4392 5871.9 1 6.265 30.2 1 K.SLKNVTEKNREIYKQLNDRQEEISR.L * Jamie_Ashy_SPB_16.05490.05490.3 4.29 0.3357 100.0 2607.8643 2607.8398 5587.6 4 5.729 31.2 1 K.NVTEKNREIYKQLNDRQEEIS.R * Jamie_Ashy_SPB_14.04866.04866.2 1.9229 0.1819 97.8 2190.892 2192.3965 2585.7 6 3.99 46.9 1 K.NREIYKQLNDRQEEISR.L * Jamie_Ashy_SPB_14.04825.04825.4 2.8541 0.0296 69.7 2195.9365 2192.3965 5074.6 20 3.501 32.3 1 K.NREIYKQLNDRQEEISR.L * Jamie_Ashy_SPB_16.05709.05709.2 2.595 0.1156 98.8 1717.4922 1717.917 7014.7 28 4.77 42.9 1 R.DLIQTKEQVSINSNK.I * Jamie_Ashy_SPB_27.09724.09724.3 2.5791 0.0531 61.3 2814.6243 2814.106 6781.2 5 3.153 25.0 1 K.EQVSINSNKILVYESEMEQCKQR.Y * Jamie_Ashy_SPB_18.06291.06291.3 3.6648 0.2734 94.7 2256.2344 2257.4895 7698.1 1 5.418 36.8 1 I.NSNKILVYESEMEQCKQR.Y * Jamie_Ashy_SPB_20.07123.07123.1 2.3593 0.3873 100.0 1528.84 1529.7114 7628.4 1 7.127 59.1 2 K.ILVYESEMEQCK.Q * Jamie_Ashy_SPB_20.07088.07088.2 4.3523 0.4368 100.0 1530.8922 1529.7114 7864.9 1 7.976 90.9 2 K.ILVYESEMEQCK.Q * Jamie_Ashy_SPB_17.05810.05810.2 2.8677 0.4172 100.0 1813.5322 1814.0297 6210.2 1 7.137 53.8 2 K.ILVYESEMEQCKQR.Y * Jamie_Ashy_SPB_10.03403.03403.2 1.8591 0.0641 88.6 1140.0322 1138.2224 6748.1 21 3.166 62.5 1 R.YQDLSQQQK.D * Jamie_Ashy_SPB_7.02455.02455.2 3.978 0.4269 100.0 1579.5122 1580.6946 6206.9 1 7.698 83.3 1 R.YQDLSQQQKDAQK.K * Jamie_Ashy_SPB_3.00847.00847.2 4.061 0.2092 100.0 1709.3922 1708.8687 6647.4 1 5.277 65.4 1 R.YQDLSQQQKDAQKK.D * Jamie_Ashy_SPB_28.09834.09834.4 4.5747 0.3382 100.0 3390.9365 3393.776 8162.4 1 6.126 28.0 2 R.YQDLSQQQKDAQKKDIEKLTNEISDLKGK.L * Jamie_Ashy_SPB_38.13663.13663.3 3.0972 0.2602 82.5 4066.7344 4066.4685 9427.7 1 5.016 23.6 1 R.YQDLSQQQKDAQKKDIEKLTNEISDLKGKLSSAENA.N * Jamie_Ashy_SPB_40.14440.14440.3 3.1549 0.3306 100.0 5267.4243 5268.7607 6945.2 1 6.271 16.7 1 R.YQDLSQQQKDAQKKDIEKLTNEISDLKGKLSSAENANADLENKFNR.L * Jamie_Ashy_SPB_19.06534.06534.2 2.6747 0.0787 40.2 1645.1122 1645.8503 6082.0 17 3.902 50.0 1 K.DAQKKDIEKLTNEI.S * Jamie_Ashy_SPB_39.13849.13849.3 5.7812 0.5119 100.0 4151.1846 4149.5615 10138.7 1 9.531 27.1 1 K.DAQKKDIEKLTNEISDLKGKLSSAENANADLENKFNR.L * Jamie_Ashy_SPB_18.06456.06456.2 5.0555 0.4384 100.0 1832.6921 1832.1045 6875.6 1 7.352 70.0 1 K.KDIEKLTNEISDLKGK.L * Jamie_Ashy_SPB_36.12808.12808.3 2.6514 0.1663 89.5 3708.1443 3707.089 7099.4 7 3.994 19.5 1 K.KDIEKLTNEISDLKGKLSSAENANADLENKFNR.L * Jamie_Ashy_SPB_14.04794.04794.2 3.697 0.2972 100.0 1218.4722 1218.3928 6412.6 1 5.706 90.0 1 K.LTNEISDLKGK.L * Jamie_Ashy_SPB_32.11340.11340.4 3.3303 0.2633 98.5 3095.8965 3093.3774 7863.3 1 4.209 24.7 1 K.LTNEISDLKGKLSSAENANADLENKFNR.L * Jamie_Ashy_SPB_30.10644.10644.3 4.9081 0.4365 100.0 2980.4944 2980.218 11508.1 1 7.105 29.8 1 L.TNEISDLKGKLSSAENANADLENKFNR.L * Jamie_Ashy_SPB_15.05328.05328.3 2.6839 0.258 98.7 2079.2644 2079.234 6390.6 1 3.979 38.9 1 K.GKLSSAENANADLENKFNR.L * Jamie_Ashy_SPB_13.04575.04575.1 2.9597 0.4104 100.0 1475.54 1476.54 4523.3 1 7.332 61.5 1 K.LSSAENANADLENK.F * Jamie_Ashy_SPB_13.04651.04651.2 3.3446 0.2207 100.0 1477.1122 1476.54 5546.7 1 4.445 76.9 1 K.LSSAENANADLENK.F * Jamie_Ashy_SPB_16.05584.05584.2 2.8809 0.4511 100.0 1891.9122 1894.0079 6850.9 1 7.46 56.2 1 K.LSSAENANADLENKFNR.L * Jamie_Ashy_SPB_16.05635.05635.3 2.458 0.1499 87.9 1892.9944 1894.0079 6118.9 3 4.091 35.9 1 K.LSSAENANADLENKFNR.L * Jamie_Ashy_SPB_1.00331.00331.2 4.2757 0.4621 100.0 1496.3322 1496.7501 5531.0 1 8.417 87.5 1 R.LKKQAHEKLDASK.K * Jamie_Ashy_SPB_1.00328.00328.2 3.6528 0.3654 100.0 1625.2322 1624.9242 5711.1 1 6.691 73.1 1 R.LKKQAHEKLDASKK.Q * Jamie_Ashy_SPB_1.00298.00298.1 2.8364 0.3745 100.0 1254.43 1255.4166 3648.7 1 6.726 65.0 1 K.KQAHEKLDASK.K * Jamie_Ashy_SPB_1.00292.00292.2 4.1409 0.3235 100.0 1383.3722 1383.5907 4272.9 1 7.837 90.9 1 K.KQAHEKLDASKK.Q * Jamie_Ashy_SPB_44.15758.15758.3 2.332 0.1281 76.4 4589.2744 4590.1504 5778.4 16 3.837 14.7 1 K.QAHEKLDASKKQQAALTNELNELKAIKDKLEQDLHFENAK.V * Jamie_Ashy_SPB_47.16892.16892.3 4.7554 0.4037 100.0 3483.3843 3481.931 8295.9 1 6.92 27.6 2 K.KQQAALTNELNELKAIKDKLEQDLHFENAK.V * Jamie_Ashy_SPB_44.15548.15548.3 5.9184 0.3683 100.0 2742.1143 2741.0732 9104.4 1 7.124 35.2 1 T.NELNELKAIKDKLEQDLHFENAK.V * Jamie_Ashy_SPB_43.15403.15403.4 3.4957 0.166 40.7 2743.8965 2741.0732 4387.7 14 4.813 25.0 1 T.NELNELKAIKDKLEQDLHFENAK.V * Jamie_Ashy_SPB_21.07368.07368.3 3.1632 0.1332 93.8 1899.5944 1900.1417 6344.8 1 4.199 40.0 1 K.AIKDKLEQDLHFENAK.V * Jamie_Ashy_SPB_16.05767.05767.2 4.2861 0.4313 100.0 1586.1122 1587.7294 9311.9 1 7.858 79.2 1 K.DKLEQDLHFENAK.V * Jamie_Ashy_SPB_16.05769.05769.1 3.2931 0.4081 100.0 1587.53 1587.7294 4720.4 1 6.76 62.5 1 K.DKLEQDLHFENAK.V * Jamie_Ashy_SPB_16.05466.05466.2 2.7464 0.1795 100.0 1046.0122 1045.2645 3460.0 1 4.476 87.5 1 K.VIDLDTKLK.A * Jamie_Ashy_SPB_16.05768.05768.3 3.4451 0.2956 100.0 3339.5942 3342.647 8642.2 1 5.414 24.1 1 K.VIDLDTKLKAHELQSEDVSRDHEKDTYR.T * Jamie_Ashy_SPB_37.13099.13099.3 2.9634 0.3368 100.0 4644.2046 4645.196 8047.1 43 5.362 14.5 1 K.VIDLDTKLKAHELQSEDVSRDHEKDTYRTLMEEIESLKK.E * Jamie_Ashy_SPB_32.11344.11344.4 3.6359 0.174 98.2 3858.4966 3860.2878 7962.5 4 4.083 19.9 1 K.LKAHELQSEDVSRDHEKDTYRTLMEEIESLKK.E * Jamie_Ashy_SPB_7.02358.02358.2 3.6878 0.3504 100.0 1271.9922 1271.3292 6100.5 1 6.696 85.0 3 K.AHELQSEDVSR.D * Jamie_Ashy_SPB_6.02031.02031.1 1.9915 0.0576 89.6 1272.56 1271.3292 5529.1 1 3.991 60.0 2 K.AHELQSEDVSR.D * Jamie_Ashy_SPB_3.00939.00939.2 2.569 0.4094 100.0 1780.4321 1780.8485 6507.7 1 6.833 53.6 1 K.AHELQSEDVSRDHEK.D * Jamie_Ashy_SPB_12.04327.04327.3 2.5258 0.0705 70.5 2317.0144 2316.4055 5425.0 1 3.388 38.9 1 K.AHELQSEDVSRDHEKDTYR.T * Jamie_Ashy_SPB_12.04233.04233.2 2.9864 0.0913 99.2 2318.132 2316.4055 6732.3 1 3.474 52.8 1 K.AHELQSEDVSRDHEKDTYR.T * Jamie_Ashy_SPB_31.10850.10850.2 2.4018 0.0238 92.3 1323.3121 1321.572 6309.9 1 2.807 80.0 1 R.TLMEEIESLKK.E * Jamie_Ashy_SPB_54.19452.19452.3 2.5624 0.1604 90.1 2080.4043 2080.4878 4366.1 53 4.016 31.2 1 R.TLMEEIESLKKELQIFK.T * Jamie_Ashy_SPB_14.04863.04863.4 2.9861 0.0708 80.8 2155.3364 2157.4282 4195.5 464 2.939 26.9 1 K.ELQIFKTANSSSDAFEKLK.V * Jamie_Ashy_SPB_31.11196.11196.3 3.1513 0.3202 95.5 2982.7744 2983.3232 3739.5 5 4.996 25.0 1 A.NSSSDAFEKLKVNMEKEKDRIIDER.T * Jamie_Ashy_SPB_16.05773.05773.3 4.3107 0.3015 100.0 2537.8145 2538.8787 7538.7 1 5.146 38.2 1 K.VNMEKEKDRIIDERTKEFEK.K * Jamie_Ashy_SPB_15.05172.05172.3 3.8036 0.159 66.1 2440.7344 2439.746 6010.4 3 4.279 33.3 1 V.NMEKEKDRIIDERTKEFEK.K * Jamie_Ashy_SPB_15.05371.05371.3 3.3091 0.0937 88.0 2875.5244 2872.1992 10783.8 6 3.558 21.9 1 K.KLQETLNKSTSSEAEYSKDIETLKK.E * Jamie_Ashy_SPB_39.13845.13845.3 5.4091 0.4686 100.0 4463.3643 4464.9243 10771.4 1 7.94 19.4 1 K.KLQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLR.R * Jamie_Ashy_SPB_39.13779.13779.4 4.2929 0.2407 100.0 4466.9365 4464.9243 9324.7 1 5.0 21.3 1 K.KLQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLR.R * Jamie_Ashy_SPB_37.13263.13263.3 5.5358 0.4695 100.0 4620.8345 4621.112 8860.0 1 7.92 26.4 1 K.KLQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Ashy_SPB_37.13230.13230.4 5.3017 0.346 100.0 4621.0166 4621.112 10004.5 2 5.853 19.4 1 K.KLQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Ashy_SPB_18.06355.06355.3 5.0166 0.4553 100.0 2743.1943 2744.0251 9074.5 1 8.222 37.0 1 K.LQETLNKSTSSEAEYSKDIETLKK.E * Jamie_Ashy_SPB_18.06346.06346.2 3.9164 0.3769 100.0 2745.2722 2744.0251 6188.3 1 7.244 41.3 1 K.LQETLNKSTSSEAEYSKDIETLKK.E * Jamie_Ashy_SPB_38.13622.13622.4 6.1739 0.4193 100.0 4492.8965 4492.938 9898.5 1 6.838 21.8 1 K.LQETLNKSTSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Ashy_SPB_15.05371.05371.2 4.5291 0.2267 100.0 1917.3522 1917.0771 10783.8 1 6.662 59.4 1 K.STSSEAEYSKDIETLKK.E * Jamie_Ashy_SPB_39.13896.13896.4 5.7241 0.4914 100.0 3663.6965 3665.9897 7701.1 1 8.811 28.2 2 K.STSSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Ashy_SPB_39.13856.13856.3 2.6696 0.1259 17.1 3477.3542 3477.8064 9901.5 2 3.724 21.3 1 T.SSEAEYSKDIETLKKEWLKEYEDETLRR.I * Jamie_Ashy_SPB_19.06643.06643.2 2.627 0.2165 84.6 1868.0122 1868.0966 6712.4 5 4.819 50.0 1 L.KKEWLKEYEDETLR.R * Jamie_Ashy_SPB_23.08065.08065.3 2.418 0.2355 96.4 1765.9443 1767.9359 6657.5 24 5.088 37.5 1 K.EWLKEYEDETLRR.I * Jamie_Ashy_SPB_7.02226.02226.3 4.9589 0.3533 100.0 1513.9443 1514.7684 6155.1 1 6.176 65.9 4 R.RIKEAEENLKKR.I * Jamie_Ashy_SPB_7.02208.02208.2 4.2712 0.3124 100.0 1514.1122 1514.7684 5315.8 1 7.055 90.9 4 R.RIKEAEENLKKR.I * Jamie_Ashy_SPB_4.01370.01370.1 2.0171 3.0E-4 86.2 1074.27 1074.2194 4508.6 6 2.869 68.8 1 R.IKEAEENLK.K * Jamie_Ashy_SPB_4.01154.01154.3 2.4406 0.1316 88.4 1357.4644 1358.5809 4395.6 9 4.018 50.0 1 R.IKEAEENLKKR.I * Jamie_Ashy_SPB_1.00375.00375.1 1.687 0.2466 100.0 1357.62 1358.5809 4440.0 5 4.812 45.0 1 R.IKEAEENLKKR.I * Jamie_Ashy_SPB_1.00367.00367.2 3.6265 0.2069 100.0 1359.0922 1358.5809 7234.8 1 5.366 85.0 5 R.IKEAEENLKKR.I * Jamie_Ashy_SPB_22.07639.07639.4 2.7226 0.0806 81.1 1813.4165 1811.1783 5237.5 6 3.741 35.7 1 R.IRLPSEERIQKIISK.R * Jamie_Ashy_SPB_13.04657.04657.1 2.3152 0.296 100.0 1365.5 1365.4857 2240.9 1 6.354 77.8 1 K.RKEELEEEFR.K * Jamie_Ashy_SPB_13.04396.04396.1 1.684 0.233 100.0 1492.53 1493.6598 3288.2 1 4.763 55.0 1 K.RKEELEEEFRK.K * Jamie_Ashy_SPB_13.04645.04645.2 2.0461 0.0079 80.4 1492.8922 1493.6598 7237.5 14 3.128 55.0 1 K.RKEELEEEFRK.K * Jamie_Ashy_SPB_13.04434.04434.3 3.3771 0.2325 100.0 1493.0944 1493.6598 6946.8 19 4.358 45.0 1 K.RKEELEEEFRK.K * Jamie_Ashy_SPB_37.13214.13214.4 2.7907 0.2009 20.6 5768.5366 5768.2407 6065.0 47 4.174 13.7 1 K.RKEELEEEFRKKLKENAGSLTFLDNKGSGEDAEEELWNSPSKGNSERPSAV.A * Jamie_Ashy_SPB_13.04618.04618.2 3.0308 0.1797 100.0 1338.0322 1337.4723 7001.2 17 4.077 61.1 1 R.KEELEEEFRK.K * Jamie_Ashy_SPB_20.07024.07024.2 4.0058 0.3168 100.0 1550.8322 1550.7521 7870.5 1 6.355 76.9 1 K.LKENAGSLTFLDNK.G * Jamie_Ashy_SPB_32.11461.11461.3 3.8804 0.257 100.0 3266.8442 3267.487 7701.4 1 5.098 25.9 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSK.G * Jamie_Ashy_SPB_30.10821.10821.3 4.776 0.5068 100.0 4164.2046 4165.43 8051.3 1 8.348 23.7 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSKGNSERPSAV.A * Jamie_Ashy_SPB_34.12181.12181.3 4.066 0.4305 100.0 4921.974 4924.3057 8354.0 1 7.516 17.2 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSKGNSERPSAVAGFINQK.N * Jamie_Ashy_SPB_34.12198.12198.4 7.476 0.5172 100.0 4924.2563 4924.3057 8241.7 1 8.117 21.1 1 K.LKENAGSLTFLDNKGSGEDAEEELWNSPSKGNSERPSAVAGFINQK.N * Jamie_Ashy_SPB_26.09081.09081.2 2.0869 0.152 39.8 2354.3123 2353.4607 6814.6 10 4.597 32.5 1 T.FLDNKGSGEDAEEELWNSPSK.G * Jamie_Ashy_SPB_21.07477.07477.1 2.4702 0.3334 100.0 1734.66 1735.7582 5208.1 1 6.343 50.0 1 K.GSGEDAEEELWNSPSK.G * Jamie_Ashy_SPB_21.07438.07438.2 5.0494 0.3931 100.0 1735.4321 1735.7582 11416.1 1 8.002 73.3 1 K.GSGEDAEEELWNSPSK.G * Jamie_Ashy_SPB_8.02764.02764.1 1.6144 0.1399 96.4 1097.38 1098.2877 4483.0 27 3.486 62.5 1 K.NLKPQEQLK.N * Jamie_Ashy_SPB_8.02868.02868.2 2.3082 0.0152 91.1 1098.4722 1098.2877 3469.4 43 3.384 75.0 1 K.NLKPQEQLK.N * Jamie_Ashy_SPB_24.08448.08448.4 4.6233 0.3258 100.0 4407.3765 4407.813 6669.7 1 5.384 17.9 1 K.NLKPQEQLKNVKNDVSFNDSQSMVTNKENNIVDSSAAGNK.A * Jamie_Ashy_SPB_17.05949.05949.1 2.4496 0.2414 100.0 1686.34 1686.7906 4742.2 1 4.68 53.6 1 K.NDVSFNDSQSMVTNK.E * Jamie_Ashy_SPB_22.07914.07914.2 3.3285 0.4448 100.0 2986.5723 2987.1382 8364.9 1 7.731 29.6 1 K.NDVSFNDSQSMVTNKENNIVDSSAAGNK.A * Jamie_Ashy_SPB_12.04243.04243.1 2.1682 0.3731 100.0 1318.44 1319.3708 3047.9 1 6.46 54.2 1 K.ENNIVDSSAAGNK.A * Jamie_Ashy_SPB_52.18658.18658.3 6.8163 0.5751 100.0 4354.1943 4353.7944 8243.6 1 10.277 26.3 1 K.AIPTFSFGKPFFSSNTSSLQSFQNPFTASQSNINTNAPLR.T * Jamie_Ashy_SPB_52.18658.18658.4 3.5121 0.2389 88.6 5805.2563 5802.52 8243.6 124 4.256 11.3 1 K.AIPTFSFGKPFFSSNTSSLQSFQNPFTASQSNINTNAPLRTLNIQPEVAVKAAI.N * Jamie_Ashy_SPB_29.10408.10408.2 4.6168 0.3366 100.0 1984.2722 1983.099 8369.1 1 6.759 63.9 1 A.AINFSNVTDLTNNSTDGAK.I * Jamie_Ashy_SPB_6.02028.02028.1 1.85 0.1348 98.0 935.43 936.0501 4724.1 1 3.879 75.0 1 K.ITEIGSTSK.R * Jamie_Ashy_SPB_6.02184.02184.2 2.3946 0.1245 99.6 935.9322 936.0501 4408.7 12 3.724 75.0 1 K.ITEIGSTSK.R * Jamie_Ashy_SPB_12.04360.04360.3 3.0636 0.1847 95.6 2535.2043 2535.7678 4220.2 1 4.304 32.6 1 K.ITEIGSTSKRPIESGTSSDPDTKK.V * Jamie_Ashy_SPB_1.00368.00368.2 2.9898 0.2275 100.0 1618.1522 1618.741 3481.0 6 5.348 60.7 1 K.RPIESGTSSDPDTKK.V YKL042W 51 60 81.0% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * Jamie_Ashy_SPB_22.07928.07928.4 2.4306 0.0568 57.2 2479.7366 2482.7124 5996.0 9 3.469 24.2 1 R.DYNDVGSRINADKLVPEEYKR.N * Jamie_Ashy_SPB_18.06307.06307.2 2.1977 0.0123 82.8 1576.8722 1575.8052 7084.9 4 3.493 54.2 1 R.INADKLVPEEYKR.N * Jamie_Ashy_SPB_13.04615.04615.2 2.9676 0.235 96.8 1463.2122 1462.6458 4960.4 1 5.102 72.7 1 I.NADKLVPEEYKR.N * Jamie_Ashy_SPB_14.04784.04784.2 4.2421 0.357 100.0 1532.5721 1534.7123 5304.6 1 6.221 83.3 1 R.NTEFINKAVQQNK.E * Jamie_Ashy_SPB_21.07392.07392.3 4.6831 0.4076 100.0 2165.7844 2166.4417 6576.0 1 7.416 42.6 1 R.NTEFINKAVQQNKELNFK.L * Jamie_Ashy_SPB_24.08538.08538.3 5.7118 0.3635 100.0 2435.8442 2435.7886 6218.3 1 6.458 43.4 1 R.NTEFINKAVQQNKELNFKLR.E * Jamie_Ashy_SPB_17.06054.06054.1 2.7057 0.03 96.7 1278.58 1278.4479 4213.5 22 3.84 66.7 1 R.EKQNEIFELK.K * Jamie_Ashy_SPB_14.04915.04915.1 2.9146 0.02 96.5 1406.79 1406.622 4206.4 1 3.307 60.0 1 R.EKQNEIFELKK.I * Jamie_Ashy_SPB_32.11410.11410.2 3.7243 0.3639 100.0 2763.392 2762.2192 8107.0 1 6.658 38.6 1 R.SKLEKYVDITKKLEDQNLNLQIK.I * Jamie_Ashy_SPB_6.02023.02023.1 1.7684 0.0377 89.0 867.42 867.033 5919.8 47 3.222 75.0 1 K.YVDITKK.L * Jamie_Ashy_SPB_15.05211.05211.2 4.3276 0.3109 100.0 1584.8322 1584.8564 5535.3 1 5.772 87.5 1 T.KKLEDQNLNLQIK.I * Jamie_Ashy_SPB_18.06200.06200.1 2.0811 0.0328 88.9 1455.75 1456.6824 5039.0 22 3.555 50.0 1 K.KLEDQNLNLQIK.I * Jamie_Ashy_SPB_30.10497.10497.3 2.6826 0.0582 73.7 2142.9243 2142.4575 6701.6 13 3.227 32.4 1 K.KLEDQNLNLQIKISDLEK.K * Jamie_Ashy_SPB_21.07274.07274.1 2.1935 0.0589 96.9 1328.42 1328.5083 9092.2 74 3.021 50.0 1 K.LEDQNLNLQIK.I * Jamie_Ashy_SPB_14.04743.04743.1 2.9644 0.3615 100.0 1409.08 1408.5052 4904.3 1 5.739 50.0 1 I.SDLEKKLSDANST.F * Jamie_Ashy_SPB_30.10530.10530.2 4.3306 0.5144 100.0 2078.9722 2080.2275 8905.7 1 10.38 64.7 1 K.VKDPMVDDDPVSENYDQI.N * Jamie_Ashy_SPB_20.07098.07098.2 3.0899 0.4552 100.0 2811.152 2812.0833 5648.9 1 7.721 41.3 1 K.VKDPMVDDDPVSENYDQINVPKHR.A * Jamie_Ashy_SPB_19.06649.06649.4 4.1318 0.4392 100.0 3689.4966 3692.0112 7242.1 1 6.519 21.9 1 K.VKDPMVDDDPVSENYDQINVPKHRAPDATGNPR.T * Jamie_Ashy_SPB_19.06756.06756.3 3.9329 0.3008 100.0 3692.3643 3692.0112 6603.0 1 6.939 21.9 1 K.VKDPMVDDDPVSENYDQINVPKHRAPDATGNPR.T * Jamie_Ashy_SPB_1.00361.00361.2 2.7682 0.0926 47.6 1630.4922 1630.8068 4461.6 8 3.514 50.0 1 I.NVPKHRAPDATGNPR.T * Jamie_Ashy_SPB_2.00663.00663.2 2.1296 0.1879 99.6 1191.1921 1192.2797 4407.4 1 4.734 65.0 1 K.HRAPDATGNPR.T * Jamie_Ashy_SPB_1.00310.00310.1 1.4337 0.2673 100.0 1191.51 1192.2797 2351.8 15 4.941 50.0 1 K.HRAPDATGNPR.T * Jamie_Ashy_SPB_1.00308.00308.2 3.6451 0.4672 100.0 1552.6322 1553.5828 6390.0 1 8.078 80.8 7 R.TTNKVSNTSDQDSR.L * Jamie_Ashy_SPB_1.00381.00381.2 3.2703 0.3337 100.0 1794.2922 1794.9163 4711.1 1 5.963 56.7 2 R.TTNKVSNTSDQDSRLK.A * Jamie_Ashy_SPB_13.04496.04496.3 3.4627 0.2123 96.3 1421.4243 1418.5497 6608.1 4 4.775 52.3 1 T.SDQDSRLKAIER.T * Jamie_Ashy_SPB_32.11520.11520.2 3.889 0.3999 100.0 1298.2322 1297.5554 8368.0 1 7.366 85.0 1 R.TLSVLTNYVMR.S * Jamie_Ashy_SPB_41.14574.14574.3 2.9901 0.1299 88.4 2764.2544 2766.058 7806.1 255 4.153 21.9 1 R.SEDGNNDRMSPLPSPLNTILPINNR.L * Jamie_Ashy_SPB_13.04557.04557.1 1.461 0.0998 88.4 1030.98 1032.1875 3699.6 133 3.34 57.1 1 R.LNFQEPKR.Y * Jamie_Ashy_SPB_50.17812.17812.4 4.1064 0.0926 93.5 4073.1765 4072.5483 9232.3 1 3.339 22.2 1 K.RYNPTVKVNPSDDDIMMYESAELKRVEEEIEELK.R * Jamie_Ashy_SPB_49.17468.17468.3 2.5775 0.0543 59.2 4071.2043 4072.5483 9622.6 1 3.543 19.7 1 R.YNPTVKVNPSDDDIMMYESAELKRVEEEIEELKR.K * Jamie_Ashy_SPB_51.18182.18182.3 3.2985 0.4116 100.0 3369.2944 3369.7402 4531.8 4 6.883 25.0 1 K.VNPSDDDIMMYESAELKRVEEEIEELKR.K * Jamie_Ashy_SPB_33.11594.11594.2 2.9796 0.3307 100.0 1743.8322 1744.9259 7417.7 3 5.613 50.0 1 N.PSDDDIMMYESAELK.R * Jamie_Ashy_SPB_33.11838.11838.2 2.5509 0.119 49.0 2087.7122 2088.3225 6403.8 3 3.834 46.9 1 Y.ESAELKRVEEEIEELKR.K * Jamie_Ashy_SPB_22.07773.07773.3 2.8741 0.2385 82.9 1798.6144 1801.05 5899.7 30 4.748 36.5 1 A.ELKRVEEEIEELKR.K * Jamie_Ashy_SPB_22.07824.07824.2 3.5579 0.1749 97.1 1802.7322 1801.05 4783.7 1 4.57 69.2 1 A.ELKRVEEEIEELKR.K * Jamie_Ashy_SPB_22.07764.07764.4 2.8684 0.0802 17.9 1805.4565 1801.05 6640.1 9 3.671 35.9 1 A.ELKRVEEEIEELKR.K * Jamie_Ashy_SPB_20.07146.07146.3 4.4198 0.1487 96.6 1930.1044 1929.2241 4530.8 1 4.127 53.6 1 A.ELKRVEEEIEELKRK.I * Jamie_Ashy_SPB_14.05020.05020.1 2.0738 0.2836 100.0 1429.5 1430.6011 2550.7 1 5.636 55.0 1 K.RVEEEIEELKR.K * Jamie_Ashy_SPB_16.05714.05714.2 2.8115 0.1698 100.0 1119.2922 1118.2262 5572.7 1 4.456 87.5 2 R.VEEEIEELK.R * Jamie_Ashy_SPB_14.05004.05004.3 2.3053 0.0506 67.4 1276.1643 1274.4136 2830.5 221 3.789 44.4 1 R.VEEEIEELKR.K * Jamie_Ashy_SPB_13.04626.04626.2 3.3828 0.1265 100.0 1402.1522 1402.5876 5655.8 1 4.93 80.0 1 R.VEEEIEELKRK.I * Jamie_Ashy_SPB_50.18036.18036.3 2.2693 0.1485 79.3 3921.1443 3923.5847 3760.1 430 4.003 16.4 1 R.KILVRKKHDLRKLSLNNQLQELQSMMDGDDNIK.L * Jamie_Ashy_SPB_1.00303.00303.1 1.3588 0.2447 100.0 924.61 925.1221 4166.2 5 4.198 66.7 1 R.KKHDLRK.L * Jamie_Ashy_SPB_44.15848.15848.2 4.0222 0.3679 100.0 3193.6921 3192.5837 6364.7 1 6.316 31.5 1 R.KLSLNNQLQELQSMMDGDDNIKLDNVSK.H * Jamie_Ashy_SPB_51.18378.18378.2 4.0082 0.1989 100.0 2408.892 2407.6729 9521.5 1 5.643 50.0 1 K.LSLNNQLQELQSMMDGDDNIK.L * Jamie_Ashy_SPB_50.17784.17784.3 3.7219 0.2735 100.0 3065.4543 3064.4097 8694.3 1 5.171 26.9 1 K.LSLNNQLQELQSMMDGDDNIKLDNVSK.H * Jamie_Ashy_SPB_15.05256.05256.2 4.0456 0.1737 96.4 1563.9922 1564.7075 7128.3 1 7.592 76.9 1 M.MDGDDNIKLDNVSK.H * Jamie_Ashy_SPB_1.00305.00305.2 2.9897 0.3369 100.0 1528.7322 1529.6583 4262.5 2 6.366 58.3 1 K.LDNVSKHNHATHR.H * Jamie_Ashy_SPB_16.05726.05726.3 3.3799 0.3249 100.0 3024.1143 3024.0266 6991.5 1 5.28 27.0 1 R.HSSQSSRDYSPSSDACLECSNDLYEK.N * Jamie_Ashy_SPB_26.09097.09097.2 3.8857 0.381 100.0 2255.2922 2254.2546 5288.7 1 6.637 69.4 1 R.DYSPSSDACLECSNDLYEK.N * Jamie_Ashy_SPB_20.07086.07086.2 3.1044 0.359 100.0 2522.672 2524.546 5375.8 1 6.819 50.0 2 R.DYSPSSDACLECSNDLYEKNR.V YDR356W 137 152 78.2% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Jamie_Ashy_SPB_8.02737.02737.2 2.5359 0.2089 100.0 1303.1921 1304.4429 5419.6 14 4.708 68.2 1 R.NTTQTQVVSPTK.V * Jamie_Ashy_SPB_15.05247.05247.2 2.5335 0.1233 98.8 2841.372 2840.033 7296.4 1 4.585 32.7 1 R.NTTQTQVVSPTKVPNANNGDENEGPVK.K * Jamie_Ashy_SPB_13.04632.04632.3 4.5654 0.2981 100.0 3124.8245 3124.3945 10486.6 1 6.346 27.7 1 R.NTTQTQVVSPTKVPNANNGDENEGPVKKR.Q * Jamie_Ashy_SPB_1.00392.00392.2 2.0723 0.0162 13.1 1967.4122 1967.1489 4790.1 1 2.923 47.1 1 T.KVPNANNGDENEGPVKKR.Q * Jamie_Ashy_SPB_51.18219.18219.2 3.1794 0.247 100.0 2966.652 2965.1536 9476.8 1 6.194 38.0 1 R.SIDDTIDSTRLFSEASQFDDSFPEIK.A * Jamie_Ashy_SPB_39.13788.13788.2 5.0129 0.4754 100.0 1860.4122 1861.0148 7666.4 1 8.547 76.7 1 R.LFSEASQFDDSFPEIK.A * Jamie_Ashy_SPB_35.12529.12529.3 6.1536 0.4615 100.0 2597.0942 2597.002 9282.9 1 8.548 41.7 1 R.NLIDDLKKDVPMSQPLKEQEVR.E * Jamie_Ashy_SPB_25.08998.08998.2 1.7855 0.087 17.9 999.5522 999.15515 3691.4 4 3.303 78.6 1 Q.PLKEQEVR.E * Jamie_Ashy_SPB_13.04561.04561.2 2.357 0.1722 100.0 1254.7922 1253.4874 5612.0 1 4.842 83.3 1 K.SLKHEIKELR.K * Jamie_Ashy_SPB_34.12163.12163.4 5.5394 0.3278 100.0 4034.2166 4033.3984 8180.6 1 5.198 22.4 1 K.SLKHEIKELRKEKNDTLNNYDTLEEETDDLKNR.L * Jamie_Ashy_SPB_21.07322.07322.3 2.8653 0.0765 76.7 3198.3245 3197.3965 6976.2 13 4.417 25.0 1 K.ELRKEKNDTLNNYDTLEEETDDLKNR.L * Jamie_Ashy_SPB_48.17078.17078.4 3.4197 0.0595 82.2 4439.3765 4436.831 7873.0 487 3.263 15.3 1 K.ELRKEKNDTLNNYDTLEEETDDLKNRLQALEKELDAK.N * Jamie_Ashy_SPB_26.09207.09207.2 3.6008 0.4337 100.0 2399.5322 2400.4688 7389.9 1 7.62 47.4 2 K.EKNDTLNNYDTLEEETDDLK.N * Jamie_Ashy_SPB_26.09366.09366.3 5.095 0.3123 100.0 2400.3542 2400.4688 9288.9 1 5.94 38.2 2 K.EKNDTLNNYDTLEEETDDLK.N * Jamie_Ashy_SPB_24.08508.08508.2 4.0622 0.4464 100.0 2671.632 2670.76 3913.6 1 7.613 57.1 2 K.EKNDTLNNYDTLEEETDDLKNR.L * Jamie_Ashy_SPB_52.18492.18492.4 5.1131 0.3011 100.0 3912.4565 3910.1943 6731.3 1 5.252 20.3 1 K.EKNDTLNNYDTLEEETDDLKNRLQALEKELDAK.N * Jamie_Ashy_SPB_48.17172.17172.4 4.7812 0.365 100.0 4151.5767 4152.472 6797.9 5 5.495 17.2 1 K.EKNDTLNNYDTLEEETDDLKNRLQALEKELDAKNK.I * Jamie_Ashy_SPB_48.17218.17218.3 3.5794 0.2462 98.5 4152.6543 4152.472 7477.9 382 4.577 15.4 1 K.EKNDTLNNYDTLEEETDDLKNRLQALEKELDAKNK.I * Jamie_Ashy_SPB_47.16804.16804.4 5.1068 0.4403 100.0 4720.7764 4722.134 6617.4 1 6.704 18.4 1 K.EKNDTLNNYDTLEEETDDLKNRLQALEKELDAKNKIVNSR.K * Jamie_Ashy_SPB_30.10764.10764.2 6.0929 0.4664 100.0 2144.2922 2143.1792 10348.9 1 8.588 70.6 2 K.NDTLNNYDTLEEETDDLK.N * Jamie_Ashy_SPB_54.19482.19482.3 4.9203 0.3441 100.0 3652.8542 3652.9048 9593.4 1 6.583 34.2 1 K.NDTLNNYDTLEEETDDLKNRLQALEKELDAK.N * Jamie_Ashy_SPB_49.17535.17535.4 3.8754 0.2638 100.0 4464.0967 4464.844 7014.3 61 4.479 15.8 1 K.NDTLNNYDTLEEETDDLKNRLQALEKELDAKNKIVNSR.K * Jamie_Ashy_SPB_15.05367.05367.1 2.4841 0.0796 98.1 1258.27 1258.4575 6221.7 3 4.565 60.0 1 R.LQALEKELDAK.N * Jamie_Ashy_SPB_12.04141.04141.2 3.0513 0.1434 100.0 1387.2922 1387.5791 3770.5 1 4.575 81.8 1 K.ELDAKNKIVNSR.K * Jamie_Ashy_SPB_4.01216.01216.1 1.0567 0.2908 100.0 1444.62 1445.495 3359.2 1 4.815 27.3 1 R.KVDDHSGCIEER.E * Jamie_Ashy_SPB_4.01386.01386.2 3.1572 0.366 100.0 1444.9521 1445.495 4926.3 1 6.97 77.3 1 R.KVDDHSGCIEER.E * Jamie_Ashy_SPB_4.01134.01134.3 3.2255 0.3582 100.0 1447.5844 1445.495 5229.8 1 5.97 52.3 1 R.KVDDHSGCIEER.E * Jamie_Ashy_SPB_13.04554.04554.2 2.6816 0.2488 100.0 2119.672 2119.2366 3901.2 1 4.849 59.4 1 R.KVDDHSGCIEEREQMER.K * Jamie_Ashy_SPB_9.03030.03030.1 2.1649 0.0409 96.8 859.57 859.0134 3541.8 2 3.441 83.3 1 R.KLAELER.K * Jamie_Ashy_SPB_29.10348.10348.3 6.9087 0.558 100.0 2799.2043 2799.2407 8255.5 1 9.407 42.4 1 R.KLKTVKDQVLELENNSDVQSLKLR.S * Jamie_Ashy_SPB_26.09057.09057.2 2.3196 0.0673 94.1 2160.5322 2160.3862 7755.0 1 3.608 47.2 1 K.TVKDQVLELENNSDVQSLK.L * Jamie_Ashy_SPB_42.14985.14985.2 3.1969 0.2191 100.0 2047.4521 2048.316 7109.8 35 5.654 37.5 1 R.SKEDELKNLMNELNELK.S * Jamie_Ashy_SPB_41.14476.14476.3 2.6229 0.1535 88.0 4207.6743 4209.6724 6994.9 17 4.054 18.4 1 R.SKEDELKNLMNELNELKSNAEEKDTQLEFKKNELR.K * Jamie_Ashy_SPB_41.14670.14670.4 4.8318 0.1511 98.6 4214.0967 4209.6724 9213.4 1 3.735 20.6 1 R.SKEDELKNLMNELNELKSNAEEKDTQLEFKKNELR.K * Jamie_Ashy_SPB_16.05754.05754.3 3.2399 0.036 18.3 1591.4944 1588.8596 6875.4 205 2.732 45.8 1 D.ELKNLMNELNELK.S * Jamie_Ashy_SPB_23.07981.07981.4 3.7925 0.2546 96.2 3151.7766 3152.5034 8044.4 2 5.737 24.7 1 L.MNELNELKSNAEEKDTQLEFKKNELR.K * Jamie_Ashy_SPB_14.04750.04750.3 3.2607 0.2447 98.5 2181.6843 2180.3794 5844.3 1 4.497 42.6 1 K.SNAEEKDTQLEFKKNELR.K * Jamie_Ashy_SPB_15.05341.05341.1 2.2951 0.1247 100.0 881.35 880.9732 4910.8 6 4.065 75.0 1 K.DTQLEFK.K * Jamie_Ashy_SPB_25.08937.08937.3 2.9455 0.0309 69.2 2418.7444 2418.768 8076.3 1 3.473 31.6 1 K.RTNELNELKIKSDEMDLQLK.Q * Jamie_Ashy_SPB_29.10404.10404.3 3.7164 0.3046 100.0 2262.9844 2262.5806 8921.1 1 5.49 37.5 1 R.TNELNELKIKSDEMDLQLK.Q * Jamie_Ashy_SPB_30.10680.10680.3 2.9829 0.1617 92.7 3223.8245 3225.4937 9306.1 27 4.368 21.3 1 K.RLKDELNELETKFSENGSQSSAKENELK.M * Jamie_Ashy_SPB_30.10610.10610.4 3.4119 0.1394 93.6 3227.2566 3225.4937 9249.8 11 3.609 22.2 1 K.RLKDELNELETKFSENGSQSSAKENELK.M * Jamie_Ashy_SPB_29.10405.10405.2 2.3037 0.1596 62.0 1479.7522 1479.6702 4513.9 4 3.702 63.6 1 R.LKDELNELETKF.S * Jamie_Ashy_SPB_25.09043.09043.2 4.5702 0.4899 100.0 3068.372 3069.3062 5640.1 1 8.813 40.4 2 R.LKDELNELETKFSENGSQSSAKENELK.M * Jamie_Ashy_SPB_42.14985.14985.3 3.706 0.0585 87.6 3070.6743 3069.3062 7109.8 1 3.784 28.8 2 R.LKDELNELETKFSENGSQSSAKENELK.M * Jamie_Ashy_SPB_31.10938.10938.4 5.1363 0.2956 100.0 3445.4966 3441.8323 5462.7 7 5.02 22.4 1 R.LKDELNELETKFSENGSQSSAKENELKMLK.N * Jamie_Ashy_SPB_25.08970.08970.1 2.3872 0.2542 100.0 1503.68 1504.6775 4839.6 1 5.293 54.2 1 K.NKIAELEEEISTK.N * Jamie_Ashy_SPB_31.10956.10956.3 2.922 0.0754 80.4 2263.3145 2259.562 6883.4 7 3.402 30.3 1 K.NKIAELEEEISTKNSQLIAK.E * Jamie_Ashy_SPB_30.10590.10590.2 3.5704 0.4214 100.0 2572.632 2573.9036 6245.8 1 8.125 40.9 1 K.NKIAELEEEISTKNSQLIAKEGK.L * Jamie_Ashy_SPB_19.06757.06757.2 3.1711 0.3157 100.0 1263.0521 1262.3997 6551.7 1 5.943 75.0 1 K.IAELEEEISTK.N * Jamie_Ashy_SPB_4.01304.01304.2 2.3049 0.0046 88.8 1088.3121 1088.2493 4055.0 98 2.632 61.1 1 K.NSQLIAKEGK.L * Jamie_Ashy_SPB_48.17242.17242.2 4.3787 0.4346 100.0 2044.6721 2045.4052 6504.1 1 7.991 61.8 1 K.LASLMAQLTQLESKLNQR.D * Jamie_Ashy_SPB_13.04653.04653.3 2.5944 0.2681 98.7 2858.1843 2859.1692 6092.4 1 4.755 26.1 1 K.LNQRDSQLGSREEELKKTNDKLQK.D * Jamie_Ashy_SPB_16.05732.05732.4 2.7835 0.0272 55.7 3242.8564 3243.6047 4739.6 163 3.155 20.5 1 K.LNQRDSQLGSREEELKKTNDKLQKDIR.I * Jamie_Ashy_SPB_13.04692.04692.3 4.7149 0.3189 100.0 2346.2644 2347.5876 8838.1 1 6.02 39.5 2 R.DSQLGSREEELKKTNDKLQK.D * Jamie_Ashy_SPB_17.05914.05914.2 3.5265 0.2974 100.0 2731.132 2732.0232 5197.5 1 6.767 43.2 1 R.DSQLGSREEELKKTNDKLQKDIR.I * Jamie_Ashy_SPB_17.05910.05910.3 5.0337 0.3431 100.0 2731.4343 2732.0232 3811.4 1 6.671 38.6 1 R.DSQLGSREEELKKTNDKLQKDIR.I * Jamie_Ashy_SPB_17.06012.06012.4 3.3013 0.0089 69.1 2735.4565 2732.0232 4386.2 1 4.061 31.1 1 R.DSQLGSREEELKKTNDKLQKDIR.I * Jamie_Ashy_SPB_3.00777.00777.2 2.439 0.1072 99.2 1230.9122 1231.3945 5040.9 66 3.977 66.7 1 K.TNDKLQKDIR.I * Jamie_Ashy_SPB_3.00840.00840.2 2.5965 0.1474 100.0 1433.3522 1433.5614 4124.4 1 4.819 68.2 2 R.IAREETVSKDER.I * Jamie_Ashy_SPB_14.05011.05011.4 3.8587 0.2333 99.2 2500.0166 2499.9138 8301.4 49 4.529 28.3 1 R.IAREETVSKDERIIDLQKKVK.Q * Jamie_Ashy_SPB_26.09067.09067.2 2.1299 0.0666 92.6 1446.4722 1446.73 6450.1 12 3.3 54.5 1 K.VKQLENDLFVIK.K * Jamie_Ashy_SPB_11.03967.03967.3 2.6871 0.1788 93.5 1947.4744 1948.0941 5518.4 4 4.435 35.9 1 K.KTHSESKTITDNELESK.D * Jamie_Ashy_SPB_14.04804.04804.2 2.7221 0.2839 96.6 2317.3523 2316.5706 4498.5 21 6.154 34.2 1 T.HSESKTITDNELESKDKLIK.I * Jamie_Ashy_SPB_43.15268.15268.3 2.9267 0.1258 87.9 3131.6343 3129.5754 8213.3 50 3.672 22.1 1 K.TITDNELESKDKLIKILENDLKVAQEK.Y * Jamie_Ashy_SPB_17.05937.05937.2 4.8935 0.4211 100.0 1779.9321 1779.0435 6541.7 1 7.147 75.0 1 K.ILENDLKVAQEKYSK.M * Jamie_Ashy_SPB_1.00319.00319.1 2.2333 0.1547 100.0 953.48 953.0831 4793.7 1 4.419 71.4 1 K.VAQEKYSK.M * Jamie_Ashy_SPB_13.04527.04527.3 2.9047 0.1773 95.6 1998.5643 1997.3171 7372.4 1 3.891 43.3 1 K.VAQEKYSKMEKELKER.E * Jamie_Ashy_SPB_17.06106.06106.2 1.7119 3.0E-4 63.4 2678.7922 2679.063 7700.6 1 4.129 32.5 1 K.VAQEKYSKMEKELKEREFNYK.I * Jamie_Ashy_SPB_10.03480.03480.2 2.8519 0.2195 100.0 1440.5721 1441.6855 3001.0 1 6.337 70.0 2 K.YSKMEKELKER.E * Jamie_Ashy_SPB_10.03396.03396.3 2.9692 0.0825 89.8 1444.0443 1441.6855 5855.6 2 4.008 47.5 1 K.YSKMEKELKER.E * Jamie_Ashy_SPB_15.05251.05251.2 2.0807 0.1309 96.8 2124.0122 2123.4314 7145.8 3 4.013 43.3 1 K.YSKMEKELKEREFNYK.I * Jamie_Ashy_SPB_23.08019.08019.3 3.4057 0.1682 95.5 3969.3542 3969.453 6718.2 1 4.124 21.8 1 K.YSKMEKELKEREFNYKISESKLEDEKTTLNEK.I * Jamie_Ashy_SPB_2.00597.00597.2 2.0093 0.0768 94.8 1063.1921 1063.2573 4169.5 4 3.914 78.6 1 K.MEKELKER.E * Jamie_Ashy_SPB_20.06968.06968.4 3.8763 0.0091 78.3 3589.8965 3591.0247 7892.7 71 3.512 19.6 1 K.MEKELKEREFNYKISESKLEDEKTTLNEK.I * Jamie_Ashy_SPB_34.11944.11944.3 2.9905 0.2374 98.7 4471.4043 4471.963 6933.6 1 4.47 18.9 1 K.ELKEREFNYKISESKLEDEKTTLNEKISNLAAENSQLK.N * Jamie_Ashy_SPB_18.06314.06314.2 2.4948 0.3992 100.0 2830.7922 2832.0935 4830.8 1 6.693 40.9 1 K.EREFNYKISESKLEDEKTTLNEK.I * Jamie_Ashy_SPB_20.06993.06993.3 5.1449 0.157 99.2 2549.7544 2546.7905 6350.6 1 4.753 45.0 1 R.EFNYKISESKLEDEKTTLNEK.I * Jamie_Ashy_SPB_13.04664.04664.2 4.5938 0.2898 100.0 1866.4521 1865.0447 8450.2 1 5.721 70.0 1 K.ISESKLEDEKTTLNEK.I * Jamie_Ashy_SPB_29.10434.10434.4 4.0192 0.2214 99.2 3137.2166 3134.465 8083.2 1 4.436 24.7 1 K.ISESKLEDEKTTLNEKISNLAAENSQLK.N * Jamie_Ashy_SPB_15.05199.05199.2 2.7169 0.0429 97.1 1289.3121 1288.4435 5475.0 3 3.804 68.2 1 K.ISNLAAENSQLK.N * Jamie_Ashy_SPB_17.06031.06031.3 3.2444 0.2174 63.8 2501.4243 2499.697 6988.6 6 5.132 25.0 1 K.ISNLAAENSQLKNKIEDNSTATH.H * Jamie_Ashy_SPB_11.03943.03943.3 2.2443 0.0195 43.1 2048.7544 2048.1914 4838.8 39 2.932 35.9 1 K.IEDNSTATHHMKENYEK.Q * Jamie_Ashy_SPB_33.11592.11592.3 3.5947 0.2795 100.0 3253.3743 3254.5725 5942.7 3 5.531 26.0 1 K.QLESLRKDIEEYKESAKDSEDKIEELK.I * Jamie_Ashy_SPB_20.07207.07207.3 3.8535 0.4775 100.0 2951.5144 2953.2761 4064.0 1 7.142 33.7 1 L.RKDIEEYKESAKDSEDKIEELKIR.I * Jamie_Ashy_SPB_6.01854.01854.2 3.8525 0.2802 100.0 1341.4722 1340.4729 4642.9 3 5.952 75.0 1 R.KDIEEYKESAK.D * Jamie_Ashy_SPB_18.06469.06469.3 4.8285 0.2966 100.0 2527.4043 2527.7417 9053.4 1 6.254 43.8 1 R.KDIEEYKESAKDSEDKIEELK.I * Jamie_Ashy_SPB_33.11592.11592.2 1.7152 0.1221 14.3 2169.2522 2171.3196 5942.7 466 3.547 26.5 1 I.EEYKESAKDSEDKIEELK.I * Jamie_Ashy_SPB_16.05688.05688.3 3.2053 0.2536 100.0 1475.4844 1475.639 5324.2 1 5.472 54.5 1 K.DSEDKIEELKIR.I * Jamie_Ashy_SPB_15.05120.05120.2 2.8806 0.2066 97.2 1361.1921 1360.5504 5710.2 1 5.013 80.0 1 D.SEDKIEELKIR.I * Jamie_Ashy_SPB_2.00595.00595.3 2.3955 0.131 88.1 1333.8243 1332.4996 4218.2 52 3.908 40.9 1 R.IAENSAKVSEKR.S * Jamie_Ashy_SPB_3.00858.00858.2 1.9652 0.0536 86.5 1333.9521 1332.4996 6188.3 1 2.996 68.2 1 R.IAENSAKVSEKR.S * Jamie_Ashy_SPB_42.14923.14923.4 6.7477 0.4506 100.0 3959.4565 3960.4282 8726.9 1 8.014 28.8 1 R.SKDIKQKDEQISDLTQNLKLQEDEISSLKSIIDR.Y * Jamie_Ashy_SPB_42.14988.14988.3 4.7725 0.4183 100.0 3960.0244 3960.4282 9009.9 1 6.821 22.7 1 R.SKDIKQKDEQISDLTQNLKLQEDEISSLKSIIDR.Y * Jamie_Ashy_SPB_46.16483.16483.4 5.1714 0.3015 100.0 3745.7766 3745.176 7224.6 2 5.748 20.4 1 K.DIKQKDEQISDLTQNLKLQEDEISSLKSIIDR.Y * Jamie_Ashy_SPB_16.05790.05790.2 3.1549 0.2377 100.0 1163.5721 1162.2823 4087.6 2 4.665 88.9 1 K.LQEDEISSLK.S * Jamie_Ashy_SPB_61.21739.21739.4 3.7473 0.1576 96.4 4154.0166 4154.577 7856.5 6 4.11 17.6 1 K.DFNQLKSEQSNIQHDLNLQILNLENKLIESEDELK.S * Jamie_Ashy_SPB_59.21031.21031.4 3.0409 0.1177 87.8 6037.9766 6038.7026 9766.7 18 3.445 11.9 1 K.DFNQLKSEQSNIQHDLNLQILNLENKLIESEDELKSLRDSQKIEIENWKR.K * Jamie_Ashy_SPB_38.13652.13652.2 5.5289 0.4378 100.0 2350.5522 2351.5786 9329.2 1 8.001 60.5 1 K.SEQSNIQHDLNLQILNLENK.L * Jamie_Ashy_SPB_58.20730.20730.4 5.0953 0.3479 100.0 5136.8564 5136.6816 10398.5 12 5.395 15.1 1 K.SEQSNIQHDLNLQILNLENKLIESEDELKSLRDSQKIEIENWK.R * Jamie_Ashy_SPB_18.06494.06494.3 2.5834 0.1894 17.7 2147.5745 2144.4302 8323.7 17 4.576 30.9 1 I.LNLENKLIESEDELKSLR.D * Jamie_Ashy_SPB_25.08995.08995.2 3.8508 0.3121 100.0 1674.5922 1674.8456 7638.1 1 5.575 65.4 1 L.NLENKLIESEDELK.S * Jamie_Ashy_SPB_33.11871.11871.2 3.1408 0.3426 100.0 2029.4321 2031.2706 6273.8 1 6.321 46.9 1 L.NLENKLIESEDELKSLR.D * Jamie_Ashy_SPB_33.11899.11899.3 3.6838 0.0953 19.3 2033.2144 2031.2706 5135.7 1 4.325 39.1 1 L.NLENKLIESEDELKSLR.D * Jamie_Ashy_SPB_31.10974.10974.3 4.3341 0.1739 91.2 2491.4343 2489.7422 8285.4 3 4.483 35.0 1 L.NLENKLIESEDELKSLRDSQK.I * Jamie_Ashy_SPB_37.13351.13351.3 2.903 0.1344 14.6 3557.3044 3558.9707 8917.5 1 3.766 23.2 1 L.NLENKLIESEDELKSLRDSQKIEIENWKR.K * Jamie_Ashy_SPB_18.06494.06494.2 3.2354 0.3077 100.0 1432.0521 1432.614 8323.7 1 7.194 86.4 1 K.LIESEDELKSLR.D * Jamie_Ashy_SPB_31.10896.10896.3 5.9431 0.4237 100.0 2802.2043 2804.1265 7999.9 1 7.149 42.0 1 K.LIESEDELKSLRDSQKIEIENWK.R * Jamie_Ashy_SPB_28.09836.09836.4 3.5045 0.2228 98.6 2958.1365 2960.314 4573.8 7 4.706 25.4 1 K.LIESEDELKSLRDSQKIEIENWKR.K * Jamie_Ashy_SPB_18.06416.06416.2 2.637 0.1563 100.0 1390.5122 1390.5358 5403.0 1 4.366 75.0 1 R.DSQKIEIENWK.R * Jamie_Ashy_SPB_13.04483.04483.2 2.2731 0.2049 100.0 1521.5122 1522.6609 4547.6 20 4.49 59.1 1 K.RKYNNLSLENDR.L * Jamie_Ashy_SPB_23.08280.08280.4 2.7937 0.0913 78.1 3835.5366 3836.2542 9098.7 14 3.263 18.3 1 K.RKYNNLSLENDRLLTEKESASDKEREISILNR.K * Jamie_Ashy_SPB_22.07892.07892.2 2.89 0.0929 99.2 1823.3522 1823.0128 6644.1 211 3.671 39.3 1 K.YNNLSLENDRLLTEK.E * Jamie_Ashy_SPB_26.09390.09390.4 4.1022 0.3527 100.0 3553.4165 3551.8926 6742.0 1 6.09 23.6 1 K.YNNLSLENDRLLTEKESASDKEREISILNR.K * Jamie_Ashy_SPB_27.09414.09414.3 3.1255 0.1145 88.5 3553.5842 3551.8926 6111.1 11 3.825 20.7 1 K.YNNLSLENDRLLTEKESASDKEREISILNR.K * Jamie_Ashy_SPB_14.04884.04884.2 4.5178 0.4668 100.0 2278.132 2279.57 7275.7 1 8.126 67.6 1 R.KLDEMDKEKWNLQESKEK.Y * Jamie_Ashy_SPB_14.05060.05060.3 3.5074 0.0925 92.3 2280.2344 2279.57 4957.9 99 3.6 29.4 1 R.KLDEMDKEKWNLQESKEK.Y * Jamie_Ashy_SPB_7.02430.02430.3 1.6602 0.0351 35.1 1214.3344 1214.4135 2678.5 229 3.249 36.1 1 K.VITANDRLRR.E * Jamie_Ashy_SPB_7.02550.02550.2 3.907 0.4133 100.0 1743.3722 1745.8467 4562.0 1 7.509 76.9 2 R.REKEELNENSNNIR.I * Jamie_Ashy_SPB_7.02361.02361.3 3.8175 0.1634 98.6 1747.8544 1745.8467 6930.7 1 4.022 50.0 2 R.REKEELNENSNNIR.I * Jamie_Ashy_SPB_12.04308.04308.2 3.1548 0.2989 100.0 1588.6522 1589.6592 3878.6 1 6.235 83.3 3 R.EKEELNENSNNIR.I * Jamie_Ashy_SPB_12.04074.04074.3 3.4747 0.2294 99.2 1589.7843 1589.6592 3763.3 2 4.767 47.9 1 R.EKEELNENSNNIR.I * Jamie_Ashy_SPB_15.05294.05294.2 2.6353 0.3045 100.0 2205.3123 2206.3894 4355.8 8 5.506 41.2 1 R.EKEELNENSNNIRIMEDK.M * Jamie_Ashy_SPB_8.02754.02754.2 1.4527 0.0324 65.9 1025.0922 1024.2385 3577.4 74 3.443 71.4 1 R.IMEDKMTR.I * Jamie_Ashy_SPB_28.09816.09816.2 3.7765 0.3766 100.0 1824.8322 1824.9854 7413.7 1 6.713 67.9 1 K.KNYLSEITSLQEENR.R * Jamie_Ashy_SPB_34.12074.12074.2 4.2443 0.2942 100.0 1698.5721 1696.8113 8783.8 1 6.724 76.9 1 K.NYLSEITSLQEENR.R * Jamie_Ashy_SPB_31.11192.11192.3 3.4852 0.2583 99.2 2382.7744 2380.5767 6349.4 4 4.775 33.3 1 K.NYLSEITSLQEENRRLEER.L * Jamie_Ashy_SPB_43.15421.15421.2 4.3025 0.4694 100.0 1920.3322 1921.0858 8552.0 1 8.208 66.7 1 K.DNDSTMQLNDIISYYK.L * Jamie_Ashy_SPB_1.00337.00337.1 1.489 0.1188 96.6 790.5 790.854 3603.7 2 4.366 70.0 1 K.YHSEVR.H * Jamie_Ashy_SPB_23.08088.08088.2 5.884 0.4145 100.0 2055.4722 2054.1777 9345.5 1 8.292 76.5 2 R.KGEHSLNISLPDDDELDR.D * Jamie_Ashy_SPB_28.09890.09890.2 3.9716 0.4253 100.0 3352.872 3353.5425 5971.8 1 8.143 25.9 1 R.KGEHSLNISLPDDDELDRDYYNSHVYTR.Y * Jamie_Ashy_SPB_28.09822.09822.4 4.9683 0.1391 98.6 3357.4966 3353.5425 4863.8 1 4.143 30.2 1 R.KGEHSLNISLPDDDELDRDYYNSHVYTR.Y * Jamie_Ashy_SPB_27.09528.09528.2 3.7535 0.4423 100.0 1926.5721 1926.0038 7627.4 1 7.3 65.6 1 K.GEHSLNISLPDDDELDR.D * Jamie_Ashy_SPB_27.09487.09487.2 2.9321 0.3511 100.0 2318.2922 2318.3716 6258.8 1 5.963 38.9 1 I.SLPDDDELDRDYYNSHVYT.R * Jamie_Ashy_SPB_23.08144.08144.2 2.0566 0.1465 59.6 977.39215 974.9567 4348.0 1 3.982 92.9 1 L.PDDDELDR.D * Jamie_Ashy_SPB_14.04861.04861.1 2.2496 0.3181 100.0 1317.81 1318.388 4123.1 1 5.916 61.1 1 R.DYYNSHVYTR.Y * Jamie_Ashy_SPB_16.05605.05605.2 2.4367 0.1204 99.6 1258.3522 1256.3599 5168.2 6 4.261 68.8 2 R.YHDYEYPLR.F YOR257W 17 18 76.4% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * Jamie_Ashy_SPB_26.09255.09255.2 4.2585 0.2974 100.0 1861.6721 1860.0282 8664.2 1 5.965 68.8 1 R.SSLQSGPLNSELLEEQK.Q * Jamie_Ashy_SPB_56.19861.19861.2 3.0876 0.2166 100.0 2940.4321 2940.21 6592.8 1 5.669 34.8 1 K.QEIYEAFSLFDMNNDGFLDYHELK.V * Jamie_Ashy_SPB_56.19867.19867.3 3.6117 0.3704 100.0 2941.6443 2940.21 7542.7 1 5.908 27.2 1 K.QEIYEAFSLFDMNNDGFLDYHELK.V * Jamie_Ashy_SPB_46.16558.16558.2 2.4942 0.2258 18.2 2229.4922 2229.4688 8103.2 22 4.278 33.3 1 F.SLFDMNNDGFLDYHELKVA.M * Jamie_Ashy_SPB_59.21217.21217.4 7.0722 0.5503 100.0 4549.3364 4551.1855 10019.1 1 8.754 25.7 1 K.ALGFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEK.I * Jamie_Ashy_SPB_59.21243.21243.3 2.4579 0.0776 65.7 4550.304 4551.1855 11037.1 20 3.002 16.2 1 K.ALGFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEK.I * Jamie_Ashy_SPB_57.20410.20410.4 5.6399 0.1617 96.0 4367.5767 4366.9473 8117.2 1 5.145 25.2 1 L.GFELPKREILDLIDEYDSEGRHLMKYDDFYIVMGEK.I * Jamie_Ashy_SPB_37.13143.13143.3 3.7555 0.3159 100.0 1825.5243 1823.9542 5026.0 5 5.869 39.3 1 K.REILDLIDEYDSEGR.H * Jamie_Ashy_SPB_43.15199.15199.1 1.3664 0.264 100.0 1666.77 1667.7667 2706.5 1 4.61 42.3 1 R.EILDLIDEYDSEGR.H * Jamie_Ashy_SPB_33.11787.11787.1 2.3095 0.3144 100.0 1380.46 1380.5549 7896.5 1 5.988 60.0 1 K.YDDFYIVMGEK.I * Jamie_Ashy_SPB_30.10770.10770.2 2.4244 0.3247 100.0 1219.0322 1217.379 6969.1 1 5.246 77.8 1 Y.DDFYIVMGEK.I * Jamie_Ashy_SPB_18.06370.06370.3 3.3503 0.1682 98.8 1863.6543 1860.0746 7110.2 1 4.605 41.7 1 R.RVAKELGETLTDEELR.A * Jamie_Ashy_SPB_66.23773.23773.3 2.2234 0.133 75.6 4791.1143 4790.1406 9561.8 83 4.198 13.4 1 R.RVAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTDS.- * Jamie_Ashy_SPB_20.07104.07104.2 4.7947 0.533 100.0 1703.3322 1703.8871 7494.4 1 9.224 82.1 1 R.VAKELGETLTDEELR.A * Jamie_Ashy_SPB_65.23203.23203.3 3.6411 0.2121 74.4 4169.9346 4170.5425 8011.5 1 5.315 19.4 1 R.VAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAI.C * Jamie_Ashy_SPB_68.24290.24290.3 2.9922 0.2047 96.0 4635.3545 4633.953 5863.4 1 4.782 19.4 2 R.VAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTDS.- * Jamie_Ashy_SPB_22.07741.07741.1 2.0867 0.2523 100.0 1405.74 1405.5016 2030.8 2 4.772 50.0 1 K.ELGETLTDEELR.A YPL124W 18 19 74.3% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Jamie_Ashy_SPB_2.00592.00592.2 1.9182 0.1356 96.8 1367.6522 1367.5913 7439.1 1 3.751 70.0 1 R.VRKEHEEALKK.L * Jamie_Ashy_SPB_7.02421.02421.2 2.0539 0.1628 98.8 1382.3722 1381.6182 6688.5 55 4.089 55.0 1 R.KEHEEALKKLR.E * Jamie_Ashy_SPB_13.04578.04578.2 3.2214 0.3654 100.0 1684.3922 1684.757 4654.9 1 6.132 67.9 1 R.EENFSSNTSELGNKK.H * Jamie_Ashy_SPB_78.28093.28093.3 2.054 0.0253 36.5 3078.2043 3082.4702 7132.1 122 2.747 19.0 1 K.KHYRAQERMSSPLHRLSPTGKSDDRK.V * Jamie_Ashy_SPB_25.08814.08814.3 2.8523 0.1592 91.2 2712.7144 2714.074 6256.6 306 3.571 22.8 1 R.QLREGNTRLPPPPFSSYGMPPTNR.S * Jamie_Ashy_SPB_21.07364.07364.2 3.0331 0.2888 100.0 2748.8123 2749.0532 6275.5 17 5.941 28.3 1 R.TSSPVRTDKFASQNVIDDQRLEIK.Y * Jamie_Ashy_SPB_21.07393.07393.4 3.6195 0.2742 100.0 2749.5764 2749.0532 7452.0 6 5.007 24.6 1 R.TSSPVRTDKFASQNVIDDQRLEIK.Y * Jamie_Ashy_SPB_20.07206.07206.3 3.4571 0.0311 81.3 2122.1643 2121.355 6610.5 1 3.532 38.2 1 R.TDKFASQNVIDDQRLEIK.Y * Jamie_Ashy_SPB_30.10584.10584.3 3.1157 0.0413 77.5 2341.4944 2338.6255 6852.9 1 3.767 36.1 2 K.FASQNVIDDQRLEIKYLER.I * Jamie_Ashy_SPB_18.06408.06408.2 2.3181 0.1109 99.2 1063.4922 1064.27 5682.9 1 3.717 85.7 1 R.LEIKYLER.I * Jamie_Ashy_SPB_30.10512.10512.2 5.1229 0.5163 100.0 1694.1721 1694.8821 7409.7 1 9.793 82.1 1 R.IVYDQGTVIDNLTSR.I * Jamie_Ashy_SPB_24.08678.08678.2 2.3383 0.0865 31.7 1320.1522 1319.4142 6806.5 1 4.684 68.2 1 Y.DQGTVIDNLTSR.I * Jamie_Ashy_SPB_40.14133.14133.2 2.3105 0.0249 87.1 2065.4521 2065.3342 5625.9 20 3.516 35.3 1 R.ITRLESFILNSISDRGDK.N * Jamie_Ashy_SPB_41.14467.14467.3 3.5774 0.1686 95.9 3108.4744 3107.4531 7633.9 1 5.902 27.9 1 R.ITRLESFILNSISDRGDKNFASLEHSR.S * Jamie_Ashy_SPB_41.14511.14511.4 4.0402 0.1301 96.0 3110.9365 3107.4531 7066.1 1 4.189 29.5 1 R.ITRLESFILNSISDRGDKNFASLEHSR.S * Jamie_Ashy_SPB_34.12234.12234.2 3.9034 0.2305 100.0 1394.9922 1394.5675 7969.1 1 6.663 81.8 1 R.LESFILNSISDR.G * Jamie_Ashy_SPB_35.12391.12391.3 2.6312 0.1211 81.0 3129.9543 3131.5073 9320.3 4 3.58 25.0 1 R.SFSGFPTNKTYGLQMGGLYENDMPYRR.S * Jamie_Ashy_SPB_1.00327.00327.2 2.0463 0.3057 100.0 1747.2322 1747.8221 2555.2 1 5.027 60.7 1 R.RSSDNINKEGAREDR.S YNL225C 66 72 66.6% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * Jamie_Ashy_SPB_55.19572.19572.2 3.7912 0.4594 100.0 2418.632 2420.6936 9768.0 1 8.157 42.5 1 K.SILPLFVNPEDVINCNFSNAR.D * Jamie_Ashy_SPB_16.05724.05724.3 5.322 0.4552 100.0 2165.6042 2166.2983 9828.8 1 8.653 50.0 1 R.DSYEENKSPSMDQMNYAR.N * Jamie_Ashy_SPB_17.06069.06069.2 2.8092 0.1726 100.0 2167.2122 2166.2983 7759.9 2 5.244 47.1 1 R.DSYEENKSPSMDQMNYAR.N * Jamie_Ashy_SPB_13.04720.04720.1 1.0542 0.123 86.4 1508.79 1509.5724 3059.4 2 3.396 41.7 1 R.NTSYQESPGLQER.P * Jamie_Ashy_SPB_13.04705.04705.2 4.0317 0.4611 100.0 1509.7322 1509.5724 7088.3 1 7.803 79.2 1 R.NTSYQESPGLQER.P * Jamie_Ashy_SPB_14.04782.04782.2 2.4093 0.2329 100.0 1734.7722 1734.8632 6647.5 33 4.93 42.9 1 R.NTSYQESPGLQERPK.N * Jamie_Ashy_SPB_13.04375.04375.3 3.9442 0.2925 100.0 3197.2144 3197.532 6756.6 1 6.107 27.8 1 T.SYQESPGLQERPKNEKDKSPIGTDVHKK.D * Jamie_Ashy_SPB_1.00370.00370.2 4.4919 0.4461 100.0 1696.6522 1696.901 6110.7 1 7.258 82.1 1 K.NEKDKSPIGTDVHKK.D * Jamie_Ashy_SPB_5.01698.01698.2 3.0924 0.2512 100.0 1198.0721 1197.3335 4963.2 9 4.996 65.0 1 K.DKSPIGTDVHK.K * Jamie_Ashy_SPB_5.01627.01627.3 3.0477 0.1777 98.7 1198.9443 1197.3335 4855.3 3 4.19 52.5 1 K.DKSPIGTDVHK.K * Jamie_Ashy_SPB_3.00828.00828.2 2.2249 0.2008 100.0 1324.9922 1325.5076 4590.5 3 4.504 68.2 1 K.DKSPIGTDVHKK.D * Jamie_Ashy_SPB_15.05193.05193.2 3.2112 0.3193 100.0 1828.4321 1828.9792 5945.6 12 5.892 50.0 1 K.DVPNFIHSTPRENSSK.H * Jamie_Ashy_SPB_18.06228.06228.2 1.9195 0.1615 48.1 1068.1921 1069.2083 4857.9 1 5.151 81.2 1 V.PNFIHSTPR.E * Jamie_Ashy_SPB_15.05354.05354.3 3.2195 0.067 12.1 4331.9644 4328.4917 9435.7 1 3.68 17.1 1 S.TPRENSSKHFTRANEQASAQPTDEHTSPDISIEDCNGAK.I * Jamie_Ashy_SPB_1.00325.00325.1 1.7184 0.255 100.0 1105.71 1106.1832 2450.2 3 5.243 56.2 1 R.ENSSKHFTR.A * Jamie_Ashy_SPB_15.05354.05354.2 5.1856 0.47 100.0 2888.3123 2886.9226 9435.7 1 7.963 42.3 1 R.ANEQASAQPTDEHTSPDISIEDCNGAK.I * Jamie_Ashy_SPB_36.12982.12982.3 4.295 0.3637 100.0 4466.574 4465.711 8218.2 1 6.726 19.9 1 R.ANEQASAQPTDEHTSPDISIEDCNGAKIFLQNSLSKEDFR.M * Jamie_Ashy_SPB_16.05554.05554.2 2.7299 0.3781 100.0 2086.0723 2087.1272 6119.9 13 6.223 36.1 1 Q.PTDEHTSPDISIEDCNGAK.I * Jamie_Ashy_SPB_24.08420.08420.2 2.684 0.3792 100.0 1597.6921 1597.8113 5767.9 1 5.64 66.7 1 K.IFLQNSLSKEDFR.M * Jamie_Ashy_SPB_24.08496.08496.3 2.3431 0.1996 93.5 1600.9443 1597.8113 3899.5 2 4.875 50.0 1 K.IFLQNSLSKEDFR.M * Jamie_Ashy_SPB_18.06386.06386.2 1.8215 0.0579 12.4 1482.6522 1484.6519 6339.0 11 3.183 54.5 1 I.FLQNSLSKEDFR.M * Jamie_Ashy_SPB_19.06810.06810.4 3.238 0.1987 98.8 2419.9766 2419.6511 5318.1 1 4.481 28.6 1 R.ANSLQKELEAATKSNDKTLDNK.K * Jamie_Ashy_SPB_34.12003.12003.3 2.4999 0.1583 87.5 4186.5244 4187.735 8550.1 322 3.938 14.6 1 R.ANSLQKELEAATKSNDKTLDNKKKIEEQTVLIENLTK.D * Jamie_Ashy_SPB_18.06218.06218.2 2.7537 0.3367 100.0 1331.4722 1332.4967 4147.1 1 6.0 68.2 1 A.NSLQKELEAATK.S * Jamie_Ashy_SPB_24.08391.08391.2 4.0558 0.4674 100.0 2802.0923 2803.1826 4809.6 1 7.86 41.3 1 K.SNDKTLDNKKKIEEQTVLIENLTK.D * Jamie_Ashy_SPB_24.08522.08522.4 4.7765 0.2605 100.0 2806.0566 2803.1826 7550.2 1 4.388 29.7 1 K.SNDKTLDNKKKIEEQTVLIENLTK.D * Jamie_Ashy_SPB_20.07108.07108.2 4.0786 0.3995 100.0 1785.8121 1787.1069 5759.8 1 6.979 71.4 1 K.KKIEEQTVLIENLTK.D * Jamie_Ashy_SPB_44.15591.15591.4 3.3709 0.0423 78.2 2963.3364 2960.4536 7677.0 1 3.005 27.1 1 K.KIEEQTVLIENLTKDLSLNKEMLEK.A * Jamie_Ashy_SPB_19.06852.06852.2 3.4622 0.1868 100.0 1321.3322 1320.5438 5911.8 5 5.176 70.0 1 K.DLSLNKEMLEK.A * Jamie_Ashy_SPB_30.10701.10701.2 3.4192 0.336 100.0 2062.2322 2064.3154 8058.0 1 6.725 52.9 1 K.DLSLNKEMLEKANDTIQT.K * Jamie_Ashy_SPB_27.09409.09409.2 3.5939 0.2503 100.0 2192.6921 2192.4895 8494.8 2 5.687 41.7 1 K.DLSLNKEMLEKANDTIQTK.H * Jamie_Ashy_SPB_26.09121.09121.2 3.4229 0.3043 100.0 1326.7922 1327.5236 3103.2 1 7.045 86.4 1 K.HTALLSLTDSLR.K * Jamie_Ashy_SPB_23.08108.08108.2 1.9152 0.0707 15.9 1527.6721 1526.7764 5164.6 191 3.419 46.2 1 K.HTALLSLTDSLRKA.E * Jamie_Ashy_SPB_69.24856.24856.2 3.559 0.4465 100.0 2905.5122 2906.2585 6209.3 1 7.261 33.3 1 R.KAELFEIPIGILFFDLYDSEENSSK.L * Jamie_Ashy_SPB_62.22280.22280.4 5.1268 0.3618 100.0 4497.2163 4499.1167 8053.0 2 6.299 15.8 1 R.KAELFEIPIGILFFDLYDSEENSSKLDHILQEKYPNIK.G * Jamie_Ashy_SPB_19.06571.06571.2 3.1284 0.2457 100.0 1436.0721 1434.4558 6385.1 1 5.586 81.8 1 F.FDLYDSEENSSK.L * Jamie_Ashy_SPB_74.26534.26534.4 2.977 0.1059 18.7 3814.4165 3818.2908 6013.0 134 3.374 18.8 1 S.SKLDHILQEKYPNIKGFLCASQQEELSRISQR.F * Jamie_Ashy_SPB_20.06946.06946.1 1.4605 0.0429 84.9 1525.63 1525.6245 3733.9 245 3.972 33.3 1 K.GFLCASQQEELSR.I * Jamie_Ashy_SPB_27.09582.09582.4 4.3662 0.4648 100.0 3377.1765 3377.8406 6849.5 1 6.732 25.9 1 R.FKNAKAEAEDLRNELENKKIEIQTMREK.N * Jamie_Ashy_SPB_27.09675.09675.3 3.6563 0.1238 93.4 3380.2144 3377.8406 8766.9 1 4.243 28.7 1 R.FKNAKAEAEDLRNELENKKIEIQTMREK.N * Jamie_Ashy_SPB_27.09558.09558.3 5.0084 0.2469 100.0 2845.7344 2845.2004 10204.6 1 5.479 31.5 1 K.NAKAEAEDLRNELENKKIEIQTMR.E * Jamie_Ashy_SPB_15.05112.05112.2 3.9248 0.2612 100.0 1660.7122 1659.7936 5378.8 1 5.086 69.2 1 A.KAEAEDLRNELENK.K * Jamie_Ashy_SPB_23.08124.08124.4 3.5325 0.2024 65.2 2659.9365 2660.0178 6322.7 1 4.316 28.6 1 A.KAEAEDLRNELENKKIEIQTMR.E * Jamie_Ashy_SPB_21.07447.07447.4 3.5026 0.1403 20.5 2917.2166 2917.3074 4472.0 1 4.523 28.3 1 A.KAEAEDLRNELENKKIEIQTMREK.N * Jamie_Ashy_SPB_17.05887.05887.1 2.0923 0.1436 100.0 1530.54 1531.6195 5269.0 12 4.499 54.2 1 K.AEAEDLRNELENK.K * Jamie_Ashy_SPB_17.05928.05928.2 3.4544 0.2283 100.0 1530.8121 1531.6195 6745.6 2 5.043 70.8 1 K.AEAEDLRNELENK.K * Jamie_Ashy_SPB_25.08748.08748.3 5.1362 0.2558 100.0 2143.4644 2143.3586 4896.1 1 5.621 51.5 1 K.AEAEDLRNELENKKIEIQ.T * Jamie_Ashy_SPB_23.08306.08306.4 4.3079 0.3409 100.0 2791.6565 2789.1333 6075.6 1 5.461 31.8 1 K.AEAEDLRNELENKKIEIQTMREK.N * Jamie_Ashy_SPB_7.02346.02346.2 1.839 0.023 76.3 1232.2522 1232.3794 3338.7 133 3.392 60.0 1 R.EKNNTLIGTNK.T * Jamie_Ashy_SPB_11.03700.03700.2 2.5263 0.2334 100.0 2032.8522 2032.3048 5413.5 1 4.844 44.1 1 R.EKNNTLIGTNKTLSKQNK.I * Jamie_Ashy_SPB_10.03319.03319.1 2.2145 0.0168 88.7 976.47 975.0898 3131.1 35 3.129 62.5 1 K.NNTLIGTNK.T * Jamie_Ashy_SPB_12.04218.04218.2 3.9959 0.4256 100.0 1774.3322 1775.0153 5432.8 1 7.52 63.3 1 K.NNTLIGTNKTLSKQNK.I * Jamie_Ashy_SPB_14.04848.04848.3 5.0984 0.3944 100.0 2416.0144 2414.7178 8355.6 1 6.847 44.7 1 R.LGSWEKSKEKYETSLKDKEK.M * Jamie_Ashy_SPB_14.04867.04867.2 4.1723 0.412 100.0 2416.0723 2414.7178 7092.6 1 6.946 57.9 2 R.LGSWEKSKEKYETSLKDKEK.M * Jamie_Ashy_SPB_3.00846.00846.2 4.5622 0.3742 100.0 1712.7522 1713.9255 7069.7 1 6.613 76.9 6 K.SKEKYETSLKDKEK.M * Jamie_Ashy_SPB_3.00774.00774.3 4.1514 0.3998 100.0 1713.3844 1713.9255 8347.2 3 6.328 42.3 1 K.SKEKYETSLKDKEK.M * Jamie_Ashy_SPB_2.00643.00643.2 2.4628 0.2958 100.0 1239.9321 1241.3837 4346.1 2 5.353 83.3 1 K.YETSLKDKEK.M * Jamie_Ashy_SPB_24.08359.08359.2 2.003 0.2689 100.0 2289.2722 2291.625 8420.5 1 5.465 42.1 1 K.MLADAEKKTNTLSKELDNLR.S * Jamie_Ashy_SPB_20.07042.07042.1 1.6728 0.1452 96.3 1404.03 1404.5632 4714.3 1 3.772 54.5 1 K.TNTLSKELDNLR.S * Jamie_Ashy_SPB_14.04740.04740.1 2.2969 0.2086 100.0 1224.64 1224.2725 3080.3 1 5.13 65.0 1 R.FGNLEGNTSER.I * Jamie_Ashy_SPB_18.06396.06396.2 2.8761 0.1653 100.0 1680.5122 1679.8705 6237.4 92 3.911 39.3 1 R.FGNLEGNTSERITIK.N * Jamie_Ashy_SPB_36.12709.12709.2 1.7003 0.0379 73.5 1299.1921 1299.5963 5315.3 49 3.055 59.1 1 K.EIVLAVGIPYPK.L * Jamie_Ashy_SPB_12.04333.04333.3 2.795 0.0625 15.3 1567.9443 1565.7501 5367.9 1 3.793 47.7 1 T.RRMDSIDHHLER.C * Jamie_Ashy_SPB_13.04410.04410.2 2.328 0.3289 100.0 1251.6721 1253.3751 4341.9 3 5.954 72.2 1 R.MDSIDHHLER.C * Jamie_Ashy_SPB_13.04405.04405.3 2.4512 0.1721 93.8 1255.3744 1253.3751 3984.1 132 4.628 44.4 1 R.MDSIDHHLER.C * Jamie_Ashy_SPB_22.07962.07962.3 3.7801 0.0862 95.9 1560.1144 1556.7246 6655.1 1 4.106 65.9 1 R.CLDHLYDHILEK.M contaminant_KERATIN02 30 46 58.4% 622 61987 5.2 U no description * Jamie_Ashy_SPB_12.04158.04158.1 1.8666 0.236 100.0 1233.68 1233.2833 3506.4 1 4.263 43.3 1 T.SGGGGGGGLGSGGSIR.S * Jamie_Ashy_SPB_99.35524.35524.2 2.6809 0.1712 76.9 1234.5721 1233.2833 3856.8 46 4.755 50.0 8 T.SGGGGGGGLGSGGSIR.S * Jamie_Ashy_SPB_10.03492.03492.2 3.5172 0.4663 100.0 1236.0922 1236.2401 6631.7 1 7.933 83.3 2 R.FSSSSGYGGGSSR.V * Jamie_Ashy_SPB_35.12568.12568.2 2.1461 0.2358 100.0 2707.392 2706.7605 8744.7 2 5.48 22.6 1 R.GGGGSFGYSYGGGSGGGFSASSLGGGFGGGSR.G * Jamie_Ashy_SPB_27.09733.09733.4 3.5657 0.0555 17.4 3004.1365 3008.1467 5526.7 221 3.773 15.7 1 G.SGGGFGGGYGSGFGGLGGFGGGAGGGDGGILTANEK.S * Jamie_Ashy_SPB_46.16567.16567.3 2.9221 0.248 98.7 4120.344 4121.5933 8351.6 2 4.394 17.9 1 R.LASYLDKVQALEEANNDLENKIQDWYDKKGPAAIQK.N * Jamie_Ashy_SPB_61.21756.21756.4 2.932 0.2681 98.6 4825.177 4825.315 8009.2 15 4.098 14.6 1 K.NYSPYYNTIDDLKDQIVDLTVGNNKTLLDIDNTRMTLDDFR.I * Jamie_Ashy_SPB_61.21835.21835.3 2.7558 0.1968 93.4 4826.3345 4825.315 9980.3 2 3.796 16.2 1 K.NYSPYYNTIDDLKDQIVDLTVGNNKTLLDIDNTRMTLDDFR.I * Jamie_Ashy_SPB_21.07390.07390.2 2.9179 0.2328 100.0 1316.7722 1316.4539 5600.9 1 6.052 72.7 1 K.DQIVDLTVGNNK.T * Jamie_Ashy_SPB_18.06488.06488.1 2.3622 0.2477 100.0 1307.56 1308.5383 4464.2 2 5.251 55.6 1 R.IKFEMEQNLR.Q * Jamie_Ashy_SPB_18.06510.06510.3 3.1022 0.0736 92.4 1310.6943 1308.5383 5118.3 15 3.641 50.0 1 R.IKFEMEQNLR.Q * Jamie_Ashy_SPB_34.11946.11946.2 2.6752 0.2058 100.0 1852.1921 1853.1003 9260.2 3 4.304 50.0 1 K.TLNDMRQEYEQLIAK.N * Jamie_Ashy_SPB_37.13068.13068.3 6.0335 0.5831 100.0 3662.7844 3664.8784 7974.3 1 9.419 30.6 1 K.NRKDIENQYETQITQIEHEVSSSGQEVQSSAK.E * Jamie_Ashy_SPB_44.15648.15648.4 5.2322 0.4118 100.0 4389.1367 4391.7095 11687.8 27 6.807 17.1 1 K.NRKDIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLR.H * Jamie_Ashy_SPB_44.15724.15724.3 6.5654 0.5204 100.0 4392.804 4391.7095 9045.9 1 8.738 27.0 1 K.NRKDIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLR.H * Jamie_Ashy_SPB_46.16396.16396.4 5.5329 0.2673 100.0 4123.7764 4121.418 10053.8 1 4.644 20.0 1 R.KDIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLR.H * Jamie_Ashy_SPB_46.16275.16275.2 5.0389 0.5562 100.0 3265.8123 3266.413 7005.6 1 9.695 37.5 1 K.DIENQYETQITQIEHEVSSSGQEVQSSAK.E * Jamie_Ashy_SPB_51.18352.18352.3 3.1365 0.0054 59.6 3995.0044 3993.244 10887.0 2 3.414 19.1 1 K.DIENQYETQITQIEHEVSSSGQEVQSSAKEVTQLR.H * Jamie_Ashy_SPB_43.15388.15388.3 4.0987 0.2263 99.2 2568.3542 2565.8865 9948.5 6 5.12 28.6 1 K.EVTQLRHGVQELEIELQSQLSK.K * Jamie_Ashy_SPB_35.12612.12612.2 3.7846 0.5458 100.0 1838.8722 1839.0557 8180.2 1 8.338 66.7 1 R.HGVQELEIELQSQLSK.K * Jamie_Ashy_SPB_32.11266.11266.3 2.7802 0.1391 90.1 1968.2043 1967.2297 5524.0 40 3.907 31.2 1 R.HGVQELEIELQSQLSKK.A * Jamie_Ashy_SPB_36.12928.12928.2 4.0144 0.5452 100.0 3167.9722 3169.4468 6488.7 1 9.72 40.7 1 K.MRLEKEIETYHNLLEGGQEDFESSGAGK.I * Jamie_Ashy_SPB_36.12912.12912.3 6.002 0.4172 100.0 3169.6743 3169.4468 9004.1 1 7.737 38.0 1 K.MRLEKEIETYHNLLEGGQEDFESSGAGK.I * Jamie_Ashy_SPB_29.10350.10350.2 4.4539 0.4331 100.0 2512.2922 2511.6177 7315.7 1 7.12 54.5 1 K.EIETYHNLLEGGQEDFESSGAGK.I * Jamie_Ashy_SPB_119.42829.42829.2 2.0168 0.0763 89.2 1351.5922 1351.4647 7905.7 28 3.979 50.0 1 K.IGLGGRGGSGGSYGR.G * Jamie_Ashy_SPB_15.05244.05244.3 2.8622 0.1657 12.7 2245.0144 2249.2375 5307.3 43 4.069 23.1 1 G.RGSRGGSGGSYGGGGSGGGYGGGSGSR.G * Jamie_Ashy_SPB_119.42811.42811.3 2.0293 0.1058 54.9 2091.5645 2093.05 7836.9 269 4.128 22.0 1 R.GSRGGSGGSYGGGGSGGGYGGGSGSR.G * Jamie_Ashy_SPB_4.01234.01234.2 5.8286 0.334 100.0 2093.5923 2093.05 5070.9 1 10.953 60.0 1 R.GSRGGSGGSYGGGGSGGGYGGGSGSR.G * Jamie_Ashy_SPB_12.04048.04048.2 5.0865 0.3253 100.0 1793.5322 1792.7324 6439.8 1 8.973 50.0 8 R.GGSGGSYGGGGSGGGYGGGSGSR.G * Jamie_Ashy_SPB_119.42613.42613.3 4.249 0.3641 100.0 3225.5645 3225.1118 9070.9 1 5.867 22.4 2 R.GGSGGSHGGGSGFGGESGGSYGGGEEASGSGGGYGGGSGK.S YOR180C 3 3 44.6% 271 30058 8.6 U DCI1 SGDID:S000005706, Chr XV from 675167-674352, reverse complement, Verified ORF, "Peroxisomal delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, involved in fatty acid metabolism, contains peroxisome targeting signals at amino and carboxy termini" * Jamie_Ashy_SPB_72.25648.25648.4 2.4478 0.1628 17.0 5307.8164 5306.199 8396.0 16 4.077 13.6 1 -.MSSRVCYHINGPFFIIKLIDPKHLNSLTFEDFVYIALLLHKANDI.D * Jamie_Ashy_SPB_31.11044.11044.4 2.8374 0.1028 14.7 6147.177 6149.0503 5432.5 34 3.528 11.0 1 K.KVLVCCLNGPAIGLSASLVALCDIVYSQNDSVFLLFPFSNLGFVAEVGTSVTLTQKL.G * Jamie_Ashy_SPB_45.15980.15980.2 2.0141 0.1013 17.5 2199.2922 2200.4937 7929.3 2 4.182 36.1 1 K.ELIGTIITKNYQLTNTETF.N YDR298C 3 3 44.3% 212 22814 9.6 U ATP5 SGDID:S000002706, Chr IV from 1058808-1058170, reverse complement, Verified ORF, "Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein)" * Jamie_Ashy_SPB_56.20013.20013.4 2.6832 0.09 75.5 5162.8564 5162.8613 7068.7 236 3.202 13.1 1 R.LGCFEKIASDFGVLNDAHNGLLKGTVTSAEPLDPKSFKRIEKALSASK.L * Jamie_Ashy_SPB_56.20199.20199.4 2.8928 0.1159 18.4 2604.0566 2599.0923 5408.7 129 4.132 25.4 1 E.PLDPKSFKRIEKALSASKLVGQGK.S * Jamie_Ashy_SPB_83.29612.29612.4 2.9174 0.0957 14.7 4368.6567 4365.1084 9371.4 307 3.648 15.4 1 K.LENVVKPEIKGGLIVELGDKTVDLSISTKIQKLNKVLEDS.I YJR053W 20 23 41.5% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * Jamie_Ashy_SPB_44.15830.15830.3 3.4358 0.1148 10.4 4794.9844 4794.9414 9939.6 30 4.489 12.5 1 T.GTVFSNSTSAFWSNKQADDDQDMEVDQDDEFLNDFQEFQNK.K * Jamie_Ashy_SPB_47.16630.16630.2 3.2677 0.1643 100.0 3182.912 3180.2068 10981.5 1 4.892 30.0 1 K.QADDDQDMEVDQDDEFLNDFQEFQNK.K * Jamie_Ashy_SPB_29.10272.10272.2 3.4067 0.3294 100.0 1256.9122 1256.3141 4655.5 1 7.243 88.9 2 K.NDIFAEEFDR.K * Jamie_Ashy_SPB_8.02746.02746.2 2.5099 0.2498 100.0 1085.7522 1086.2767 4903.5 2 5.147 75.0 2 R.KLSLEDKPR.L * Jamie_Ashy_SPB_31.10992.10992.3 2.1213 0.1895 87.3 2444.2744 2442.66 3802.8 1 4.339 32.5 1 K.SMPNLALVNPAIREEEEDEER.E * Jamie_Ashy_SPB_57.20412.20412.3 4.0933 0.3621 100.0 4089.8342 4091.1682 8745.5 1 7.509 18.1 1 K.FSSDDEGDFLTGFEELEGEAIDETISSNDKESADHPR.F * Jamie_Ashy_SPB_61.21912.21912.2 2.4462 0.1621 60.6 2172.7322 2172.2268 8377.8 1 5.185 36.8 1 E.GEAIDETISSNDKESADHPR.F * Jamie_Ashy_SPB_16.05784.05784.3 3.7756 0.1159 96.0 2211.6843 2209.383 6127.0 1 3.959 43.8 1 R.QRDWNTQQELDSFKEKR.S * Jamie_Ashy_SPB_30.10587.10587.2 4.3408 0.3703 100.0 1512.9521 1511.5878 6353.9 1 6.856 81.8 2 R.DWNTQQELDSFK.E * Jamie_Ashy_SPB_30.10587.10587.3 2.617 0.1022 80.9 2268.9243 2267.4631 6353.9 4 3.75 27.9 1 R.DWNTQQELDSFKEKRSVR.H * Jamie_Ashy_SPB_6.02001.02001.2 2.0675 0.235 100.0 1152.0922 1153.237 5160.4 1 4.374 72.2 1 K.FSDVDTANRK.A * Jamie_Ashy_SPB_13.04513.04513.3 3.029 0.2489 100.0 1199.1843 1197.51 3154.2 3 4.426 50.0 1 K.ALLIKNKLQR.D * Jamie_Ashy_SPB_4.01302.01302.3 4.2691 0.3708 100.0 1890.8344 1891.0507 8166.2 1 7.498 50.0 1 R.DADSKKQKYSDLQHAR.A * Jamie_Ashy_SPB_4.01230.01230.4 2.4815 0.0278 54.2 1894.1765 1891.0507 6261.1 151 3.503 30.0 1 R.DADSKKQKYSDLQHAR.A * Jamie_Ashy_SPB_39.13953.13953.3 1.8248 0.0030 33.4 1661.4243 1661.8179 5354.6 444 2.978 32.7 1 K.QKYSDLQHARATSR.N * Jamie_Ashy_SPB_29.10218.10218.2 4.1704 0.3516 100.0 1516.8722 1515.768 7623.6 1 6.282 83.3 1 K.VIGNMILDEQNLR.W * Jamie_Ashy_SPB_20.07242.07242.2 3.2165 0.3313 100.0 2288.652 2288.3892 8518.2 1 7.151 42.5 1 R.SKSQVNTPFVSNDNDGVYQST.A * Jamie_Ashy_SPB_18.06501.06501.3 5.0948 0.5079 100.0 2784.5942 2785.9438 8043.3 1 9.114 35.0 1 R.SKSQVNTPFVSNDNDGVYQSTAAQAR.L * Jamie_Ashy_SPB_22.07946.07946.2 3.0637 0.4462 100.0 2569.6921 2570.6917 8538.7 1 7.831 37.0 1 K.SQVNTPFVSNDNDGVYQSTAAQAR.L * Jamie_Ashy_SPB_41.14611.14611.2 4.8947 0.4893 100.0 2356.892 2356.5146 8421.3 1 9.93 60.0 1 R.DETIISVDEETIMDESTVNSK.R Reverse_YDR493W 3 3 35.8% 123 13927 9.9 U YDR493W SGDID:S000002901, Chr IV from 1436207-1436578, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * Jamie_Ashy_SPB_23.08310.08310.2 2.25 0.0638 18.3 1654.1122 1656.8326 6510.1 2 3.136 50.0 1 R.LFVAVDELHQIQDE.T * Jamie_Ashy_SPB_117.42008.42008.2 1.5426 0.1055 11.7 2317.5923 2318.6748 7140.9 80 3.393 21.1 1 Q.IQDETTLKPDPPCLMGSRMK.A * Jamie_Ashy_SPB_103.36949.36949.2 1.608 0.0965 12.7 1632.4722 1632.863 4456.7 444 3.04 34.6 1 L.LVEADNRFAIRTAR.L YPR020W 2 2 34.8% 115 12921 9.8 U ATP20 SGDID:S000006224, Chr XVI from 599867-600214, Verified ORF, "Subunit g of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase" * Jamie_Ashy_SPB_32.11456.11456.2 1.7988 0.0377 10.1 2001.3922 2000.3458 6586.3 206 2.545 27.8 1 T.SGLVSKANLLSSKALYYGK.V * Jamie_Ashy_SPB_66.23750.23750.2 1.6891 0.1119 15.5 2399.9922 2397.8691 8605.4 49 3.694 22.5 1 I.QLIGFYSVGEIIGRRKLVGYK.H YOR373W 28 30 34.2% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Jamie_Ashy_SPB_33.11707.11707.2 3.7986 0.5618 100.0 3073.7322 3074.1975 6536.8 1 9.533 34.6 1 K.EYESNHDFQDSNFTSQVVEPAISDSVK.K * Jamie_Ashy_SPB_46.16322.16322.2 2.2658 0.3017 66.7 2033.8121 2035.1777 6121.6 166 4.577 25.0 1 P.GIGSGGGTNFGGAVGTANKVPES.D * Jamie_Ashy_SPB_1.00349.00349.1 2.2345 0.1569 100.0 885.39 886.0569 3288.3 1 4.084 83.3 1 R.HLDMKNK.T * Jamie_Ashy_SPB_12.04254.04254.2 2.054 0.0172 78.4 2411.9521 2411.6086 6276.5 1 3.099 42.5 1 K.NKTTTMQTHENHDTISISHSK.D * Jamie_Ashy_SPB_31.11019.11019.2 1.7904 0.0941 15.5 1668.6122 1668.8467 6890.7 2 3.418 43.3 1 S.GPAAEAHDVFDGILQK.Q * Jamie_Ashy_SPB_27.09488.09488.2 3.0084 0.2099 97.3 1613.6721 1611.7948 7930.4 1 4.9 50.0 1 G.PAAEAHDVFDGILQK.Q * Jamie_Ashy_SPB_26.09163.09163.2 1.8951 0.0738 15.9 1443.3121 1443.5992 6678.6 1 3.146 66.7 1 A.AEAHDVFDGILQK.Q * Jamie_Ashy_SPB_15.05302.05302.3 5.377 0.4741 100.0 4254.9243 4254.3257 6254.4 1 6.962 19.6 1 K.SNYLVGSYPSNSNNKNNNNNNNNNNNNSININNKDNAR.T * Jamie_Ashy_SPB_11.03828.03828.2 4.7787 0.3079 100.0 2171.2122 2172.152 6865.0 1 7.78 72.2 1 K.NNNNNNNNNNNNSININNK.D * Jamie_Ashy_SPB_118.42194.42194.3 2.974 0.1226 87.8 3294.1743 3293.2776 8633.9 4 4.319 21.6 1 R.TKEEDEEDTSNSFEFSSSSSMSSSQTQSGR.K * Jamie_Ashy_SPB_20.07238.07238.2 2.0431 0.0951 18.9 1606.7722 1605.9615 5365.5 157 3.869 39.3 1 K.VLKKPPLNTISPGQL.G * Jamie_Ashy_SPB_9.03072.03072.1 2.364 0.1112 100.0 872.59 872.05255 3925.3 3 3.842 78.6 1 K.APSILDKK.A * Jamie_Ashy_SPB_4.01408.01408.2 2.4935 0.0533 30.3 1082.1522 1080.1857 3804.9 3 4.425 66.7 1 T.STKPGDVGYR.Q * Jamie_Ashy_SPB_17.06037.06037.2 4.4261 0.4617 100.0 2257.2122 2258.401 8520.7 1 8.584 57.9 2 K.KIQEEENLANSDDTPLDTPK.F * Jamie_Ashy_SPB_34.12140.12140.2 4.3018 0.502 100.0 3124.5122 3124.385 7593.9 1 7.608 38.5 1 K.KIQEEENLANSDDTPLDTPKFNDLFTK.N * Jamie_Ashy_SPB_31.11078.11078.3 2.2685 0.0302 42.7 3553.7344 3552.8333 9274.5 14 3.334 18.3 1 K.KIQEEENLANSDDTPLDTPKFNDLFTKNGTR.A * Jamie_Ashy_SPB_31.11088.11088.4 4.2895 0.2488 100.0 3556.4966 3552.8333 8195.4 1 4.518 27.2 2 K.KIQEEENLANSDDTPLDTPKFNDLFTKNGTR.A * Jamie_Ashy_SPB_39.13778.13778.3 3.5424 0.241 98.5 2995.9443 2996.211 7815.0 1 4.35 27.0 1 K.IQEEENLANSDDTPLDTPKFNDLFTK.N * Jamie_Ashy_SPB_38.13502.13502.2 4.8335 0.5179 100.0 2996.4521 2996.211 7734.7 1 8.536 40.0 1 K.IQEEENLANSDDTPLDTPKFNDLFTK.N * Jamie_Ashy_SPB_20.06921.06921.2 2.617 0.1315 61.8 1520.9521 1519.738 6919.6 6 4.189 54.2 1 K.LKTFPAERVEDIT.S * Jamie_Ashy_SPB_35.12634.12634.2 2.3866 0.3366 100.0 2843.0723 2845.046 6706.0 1 6.154 33.3 1 K.TFPAERVEDITSISEVNTSFNETEK.Q * Jamie_Ashy_SPB_18.06208.06208.1 2.6756 0.4035 100.0 1470.53 1471.52 5260.7 1 6.852 62.5 1 K.LSGSPSYDSDWEK.I * Jamie_Ashy_SPB_40.14230.14230.2 2.58 0.0431 94.3 2397.672 2396.6592 7857.9 1 4.096 37.5 1 K.LSGSPSYDSDWEKILKVDLSR.G * Jamie_Ashy_SPB_11.03744.03744.2 1.8111 0.0667 89.3 912.0522 913.02136 4246.7 14 4.518 78.6 1 R.NNPITTPR.H * Jamie_Ashy_SPB_11.03660.03660.1 1.4927 0.1722 98.0 912.69 913.02136 3598.4 28 4.064 64.3 1 R.NNPITTPR.H * Jamie_Ashy_SPB_3.00816.00816.2 2.5675 0.2075 100.0 1321.0521 1321.4385 3744.5 4 4.694 70.0 1 R.NNPITTPRHDR.A * Jamie_Ashy_SPB_4.01473.01473.1 1.5966 0.1963 100.0 934.68 935.02747 3937.1 25 5.081 64.3 1 R.ASTSLHYR.Q * Jamie_Ashy_SPB_5.01665.01665.2 1.6223 0.0048 68.8 936.4522 935.02747 3354.6 161 2.891 64.3 1 R.ASTSLHYR.Q YNL038W 2 2 34.1% 229 26817 10.1 U GPI15 SGDID:S000004983, Chr XIV from 557021-557611,557686-557784, Verified ORF, "Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein" * Jamie_Ashy_SPB_70.25215.25215.4 3.1607 0.1464 11.5 3584.4966 3580.365 9008.6 236 3.429 20.8 1 N.MGIQYHQIVLILLLNILFYVICLRSRFLE.H * Jamie_Ashy_SPB_48.17198.17198.4 2.0687 0.0189 35.5 5399.3765 5395.496 6282.4 95 2.758 13.2 1 R.TFEVTIARSFQILIIMGLFALGTIILVRGPSVETVTIFKESGLQLSRVK.G Reverse_YGR135W 2 2 32.6% 258 28714 5.2 U PRE9 SGDID:S000003367, Chr VII from 761397-762173, Verified ORF, "20S proteasome beta-type subunit; the only nonessential 20S subunit" * Jamie_Ashy_SPB_118.42238.42238.3 1.8568 0.0026 33.2 4788.9844 4787.177 8835.7 215 2.628 12.8 1 K.WGTYNGSPNSTYLQYGYRDDYGAYIFSVGFPRLGGHQTYGQK.I * Jamie_Ashy_SPB_108.38661.38661.4 1.9391 0.0465 37.5 4521.8164 4520.1777 7226.4 71 3.0 15.4 1 R.ATNILIEADATLGAVAVAIKDNLKYLKETSTDQELLTSTVKR.E contaminant_KERATIN13 14 16 31.4% 643 65494 6.6 U no description * Jamie_Ashy_SPB_33.11571.11571.3 3.7539 0.2676 100.0 2427.5344 2423.7341 7321.3 1 5.12 34.2 1 R.EREQIKSLNNQFASFIDKVR.F * Jamie_Ashy_SPB_35.12300.12300.3 2.9542 0.2774 99.2 2137.8542 2138.4312 5435.2 2 5.312 33.8 1 R.EQIKSLNNQFASFIDKVR.F * Jamie_Ashy_SPB_35.12302.12302.2 3.3914 0.3403 100.0 1640.4122 1639.8516 8280.8 1 6.303 61.5 1 K.SLNNQFASFIDKVR.F Jamie_Ashy_SPB_17.05989.05989.1 1.78 0.2137 100.0 1476.17 1476.6726 6479.7 2 4.401 59.1 1 R.FLEQQNQVLQTK.W * Jamie_Ashy_SPB_40.14223.14223.3 2.6607 0.1217 82.1 2932.4343 2934.2786 7767.2 4 3.677 26.1 1 R.FLEQQNQVLQTKWELLQQVDTSTR.T * Jamie_Ashy_SPB_40.14120.14120.4 5.0106 0.2451 100.0 3278.1765 3277.605 8806.6 2 5.217 26.0 1 R.LDSELKNMQDMVEDYRNKYEDEINKR.T Jamie_Ashy_SPB_12.04278.04278.3 2.709 0.1939 98.8 1309.1943 1309.4215 4923.1 113 4.503 47.2 1 R.NKYEDEINKR.T Jamie_Ashy_SPB_5.01832.01832.2 2.1983 0.1982 81.2 1194.6921 1195.3176 4523.2 20 4.61 75.0 1 N.KYEDEINKR.T * Jamie_Ashy_SPB_8.02905.02905.2 2.0218 0.0838 94.3 1126.3121 1128.2854 5959.5 18 3.762 61.1 1 K.KDVDGAYMTK.V * Jamie_Ashy_SPB_82.29332.29332.3 3.7521 0.3943 100.0 5185.1343 5184.7993 11027.4 1 6.564 16.5 1 K.VDLQAKLDNLQQEIDFLTALYQAELSQMQTQISETNVILSMDNNR.Q * Jamie_Ashy_SPB_23.08268.08268.3 6.6053 0.4873 100.0 2504.5144 2502.7405 5321.7 1 9.515 50.0 1 K.SKAEAESLYQSKYEELQITAGR.H * Jamie_Ashy_SPB_5.01705.01705.2 1.6624 0.0232 70.2 1201.1921 1202.3964 5265.7 203 3.02 55.6 1 R.LRSEIDNVKK.Q * Jamie_Ashy_SPB_119.42633.42633.2 3.0179 0.348 100.0 2386.7722 2385.298 8613.5 1 7.16 30.0 3 R.GGGGGGYGSGGSSYGSGGGSYGSGGGGGGGR.G * Jamie_Ashy_SPB_120.43170.43170.2 1.1651 0.121 67.5 1005.53217 1006.9645 6945.7 39 3.721 46.2 1 R.GGSGGGGGGSSGGR.G contaminant_KERATIN03 12 14 31.4% 593 59519 5.2 U no description * Jamie_Ashy_SPB_13.04494.04494.2 1.679 0.0681 11.7 1063.7122 1064.1094 6111.7 4 3.586 59.1 1 G.GCGGGGGVSSLR.I * Jamie_Ashy_SPB_22.07873.07873.2 2.6132 0.222 100.0 2212.7322 2211.3525 8778.8 4 5.34 32.6 1 R.ISSSKGSLGGGFSSGGFSGGSFSR.G * Jamie_Ashy_SPB_21.07401.07401.2 4.9148 0.5482 100.0 2097.372 2098.193 7635.5 1 10.163 61.4 1 I.SSSKGSLGGGFSSGGFSGGSFSR.G * Jamie_Ashy_SPB_26.09120.09120.2 3.88 0.3925 100.0 1710.5122 1708.7844 6783.1 1 6.966 41.7 3 K.GSLGGGFSSGGFSGGSFSR.G * Jamie_Ashy_SPB_27.09422.09422.2 2.1372 0.1703 51.0 1652.7122 1651.7324 7789.8 42 3.648 38.2 1 G.SLGGGFSSGGFSGGSFSR.G * Jamie_Ashy_SPB_16.05521.05521.1 2.8703 0.2903 100.0 1381.73 1382.4668 7180.9 1 5.705 68.2 1 R.ALEESNYELEGK.I * Jamie_Ashy_SPB_15.05187.05187.2 3.0213 0.3474 100.0 1236.1921 1235.4258 3436.4 1 5.621 88.9 1 R.LKYENEVALR.Q * Jamie_Ashy_SPB_21.07414.07414.3 1.4302 0.0996 33.8 4284.2344 4284.7812 4996.3 114 3.132 10.1 1 K.KNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMR.S * Jamie_Ashy_SPB_30.10663.10663.2 1.7059 0.0862 82.7 2585.3523 2585.749 9142.8 57 3.47 27.5 1 R.SQYEQLAEQNRKDAEAWFNEK.S * Jamie_Ashy_SPB_18.06241.06241.2 1.9284 0.1169 95.1 1325.0721 1325.4203 6147.1 11 3.953 60.0 1 K.DAEAWFNEKSK.E * Jamie_Ashy_SPB_83.29809.29809.4 3.8053 0.2371 13.0 3965.9766 3964.7305 7260.1 4 4.584 16.3 1 G.GGSSGGGYGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGGSSSGGH.G * Jamie_Ashy_SPB_121.43446.43446.2 2.1662 0.1875 63.6 1340.8322 1340.3434 5807.8 1 4.427 57.1 1 K.SSSSGSVGESSSKGP.R Reverse_YER014C-A 3 3 30.7% 153 18006 7.4 U BUD25 SGDID:S000007590, Chr V from 184699-184677,184168-183730, reverse complement, Uncharacterized ORF, "Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern" * Jamie_Ashy_SPB_22.07862.07862.3 2.0435 0.0050 33.7 2827.4343 2824.298 5937.8 163 2.59 21.7 1 -.LAKSVQNGFGILIWPFQKTYNEIR.N * Jamie_Ashy_SPB_28.10056.10056.4 2.9095 0.0528 71.6 4130.8164 4129.7964 6498.9 26 2.982 17.7 1 -.LAKSVQNGFGILIWPFQKTYNEIRNDFPIEFWKK.V * Jamie_Ashy_SPB_56.20152.20152.2 1.5021 0.0924 11.7 1613.5922 1611.8065 6645.8 315 3.316 33.3 1 H.WGPLYGNICDFIR.Q Reverse_YIL027C 2 2 30.5% 141 15907 8.9 U KRE27 SGDID:S000001289, Chr IX from 304102-303677, reverse complement, Verified ORF, "Protein of unknown function; null mutant shows K1 killer toxin resistance" * Jamie_Ashy_SPB_54.19353.19353.2 2.0635 0.0723 13.0 2449.372 2449.7227 5226.8 372 3.559 25.0 1 V.FSPTFIIEGNPNSGILNDVNTAK.N * Jamie_Ashy_SPB_117.42122.42122.2 1.75 0.2432 62.5 2158.5122 2156.5486 7452.0 42 4.695 28.9 1 Y.MIDKPLKSIAGQANNLSNLK.L YGL031C 2 2 29.7% 155 17614 11.3 U RPL24A SGDID:S000002999, Chr VII from 437939-437472, reverse complement, Verified ORF, "Ribosomal protein L30 of the large (60S) ribosomal subunit, nearly identical to Rpl24Bp and has similarity to rat L24 ribosomal protein; not essential for translation but may be required for normal translation rate" Jamie_Ashy_SPB_54.19285.19285.2 1.7228 0.129 90.5 1632.8722 1630.9315 8014.8 187 3.835 34.6 1 K.KGITEEVAKKRSRK.T * Jamie_Ashy_SPB_102.36439.36439.4 2.782 0.1271 17.6 3403.6165 3397.8623 7771.4 17 3.548 20.4 1 K.KAEKAARKAEKAKSAGTQSSKFSKQQAKGAFQ.K YBL002W 2 2 28.2% 131 14237 10.1 U HTB2 SGDID:S000000098, Chr II from 236495-236890, Verified ORF, "One of two nearly identical (see HTB1) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation" YDR224C 2 2 28.2% 131 14252 10.1 U HTB1 SGDID:S000002632, Chr IV from 914706-914311, reverse complement, Verified ORF, "One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation " Jamie_Ashy_SPB_52.18565.18565.4 3.2387 0.2017 53.9 3212.3765 3207.6577 7819.8 93 4.071 20.2 1 F.ERIATEASKLAAYNKKSTISAREIQTAVR.L Jamie_Ashy_SPB_118.42402.42402.2 1.5154 0.0891 10.4 2236.7722 2239.6206 6681.8 20 3.586 27.5 1 K.STISAREIQTAVRLILPGELA.K Reverse_YLR192C 4 4 27.9% 265 29564 5.2 U HCR1 SGDID:S000004182, Chr XII from 539591-538794, reverse complement, Verified ORF, "Dual function protein involved in translation initiation as a substoichiometric component of eukaryotic translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA" * Jamie_Ashy_SPB_47.16916.16916.3 2.6969 0.0795 74.3 3404.5144 3401.5955 6497.6 109 3.074 18.1 1 -.MFDDDGFEFDDPGDLDFDQDKKFAGGLNTK.G * Jamie_Ashy_SPB_15.05106.05106.3 2.7095 0.1343 14.0 1976.8444 1976.3732 4181.8 88 3.771 30.9 1 D.QDKKFAGGLNTKGKVKKK.G * Jamie_Ashy_SPB_15.05212.05212.2 1.6725 0.1017 15.2 1147.8522 1146.347 5797.6 11 3.533 61.1 1 S.EISMPKAVDR.I * Jamie_Ashy_SPB_63.22381.22381.4 3.554 0.134 17.2 3076.7366 3078.4116 5826.3 33 3.206 23.5 1 K.LAQEEQEKQLARARPHEEAVGLGAFLDA.A Reverse_YLR029C 3 3 27.5% 204 24422 11.4 U RPL15A SGDID:S000004019, Chr XII from 202591-201977, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Bp and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA" Reverse_YMR121C 3 3 27.5% 204 24422 11.4 U RPL15B SGDID:S000004728, Chr XIII from 510347-509733, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl15Ap and has similarity to rat L15 ribosomal protein; binds to 5.8 S rRNA" Jamie_Ashy_SPB_7.02283.02283.2 1.756 0.0076 69.1 1158.8522 1160.3187 5038.0 35 2.523 60.0 1 K.KGTATLGRAER.H Jamie_Ashy_SPB_15.05211.05211.4 3.5406 0.0678 17.5 3168.6565 3167.5088 5535.3 472 3.62 20.4 1 R.GVREEATARLSRQYKLENVGQNTPKGYT.A Jamie_Ashy_SPB_27.09543.09543.4 2.6844 0.1688 15.4 2111.5764 2111.547 4287.8 171 3.76 30.2 1 V.RVRYIVFGQKAKYGLRR.A YHR044C 2 2 26.8% 246 27100 5.4 U DOG1 SGDID:S000001086, Chr VIII from 194801-194061, reverse complement, Verified ORF, "2-deoxyglucose-6-phosphate phosphatase, similar to Dog2p, member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed, in vivo substrate has not yet been identified" * Jamie_Ashy_SPB_60.21332.21332.4 2.6763 0.139 17.1 4751.2964 4757.257 7120.7 66 3.424 14.0 1 K.LDDTDNKGVLALEKDIAHSYLDTVSLIPGAENLLLSLDVDTETQ.K Jamie_Ashy_SPB_54.19414.19414.3 3.1485 0.1408 10.9 2565.2944 2562.848 7452.4 14 3.811 28.6 1 G.FDVKNGKPDPEGYSRARDLLRQ.D Reverse_YCR063W 2 2 26.1% 157 18447 9.4 U BUD31 SGDID:S000000659, Chr III from 228313-228786, Verified ORF, "Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern" * Jamie_Ashy_SPB_61.21846.21846.2 1.678 0.1813 29.5 1768.4321 1768.0292 6707.1 29 3.817 42.9 1 T.GKKRAEEELQARPVR.C * Jamie_Ashy_SPB_117.42085.42085.3 2.8863 0.1065 17.2 3048.0244 3045.5552 6395.4 33 3.899 21.0 1 Q.IEFDTLTPKIKEFGDPAPKSRRTKIR.P Reverse_YPL127C 2 2 26.0% 258 27804 10.2 U HHO1 SGDID:S000006048, Chr XVI from 309603-308827, reverse complement, Verified ORF, "Histone H1, a linker histone required for nucleosome packaging at restricted sites; suppresses DNA repair involving homologous recombination; not required for telomeric silencing, basal transcriptional repression, or efficient sporulation" * Jamie_Ashy_SPB_38.13620.13620.3 1.9837 0.0703 43.0 3597.9844 3600.151 6772.1 9 3.514 17.6 1 K.TSAAKAKSATASVSTAAQKPKPSVEKEKKVEPSKK.K * Jamie_Ashy_SPB_85.30369.30369.3 2.9203 0.1366 88.7 3336.4143 3335.8699 7126.6 1 3.865 22.6 1 K.SSAEEKKATTKKIAAKSTSKEKGKSTAPKKGK.S Reverse_YFR031C-A 2 2 26.0% 254 27408 11.1 U RPL2A SGDID:S000002104, Chr VI from 221406-221403,221255-220495, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E. coli L2 and rat L8 ribosomal proteins" Reverse_YIL018W 2 2 26.0% 254 27408 11.1 U RPL2B SGDID:S000001280, Chr IX from 316766-316769,317170-317930, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures" Jamie_Ashy_SPB_53.18769.18769.4 3.0431 0.0261 66.6 4440.177 4442.0244 7255.0 176 2.867 14.0 1 R.RAAILGAKQGSVAGRSITSAKGIHQHNGGGHPHDVPNMAVGRTK.P Jamie_Ashy_SPB_24.08377.08377.3 2.245 0.0227 41.2 2416.2244 2413.7078 6425.9 28 2.533 25.0 1 K.AAGQRLRTHSTFISGAGKRQNR.I YPR143W 2 2 25.6% 250 28212 5.7 U RRP15 SGDID:S000006347, Chr XVI from 818319-819071, Verified ORF, "Nucleolar protein, constituent of pre-60S ribosomal particles; required for processing of the 27S pre-rRNA at the A2 site to yield 5.8S and 25S rRNA" * Jamie_Ashy_SPB_80.28531.28531.2 1.5412 0.111 13.1 1736.8722 1735.9436 6514.5 6 3.443 42.3 1 K.DKKRTRKNAEFGRE.K * Jamie_Ashy_SPB_95.33963.33963.4 1.9146 0.0988 48.3 5680.9365 5682.7144 8548.2 156 3.348 12.2 1 K.KVLKQSESEKLEYKAKKALLAEKKKLLGKARKTDIIPIASGEDRSENIRK.V Reverse_YPL267W 2 2 25.4% 209 23870 9.6 U YPL267W SGDID:S000006188, Chr XVI from 38169-38798, Uncharacterized ORF, "Protein of unknown function, potential Cdc28p substrate" * Jamie_Ashy_SPB_26.09216.09216.4 2.9037 0.1779 17.6 4190.9365 4186.922 6143.0 123 3.8 15.3 1 K.STFFISIKSNRPPTIFSPLSIKKDGLPLTLPITEDTLP.D * Jamie_Ashy_SPB_75.26850.26850.2 1.757 0.1554 22.9 1640.2522 1641.7349 6991.5 1 4.626 46.4 1 K.APSLNEKVQSPNNEN.S Reverse_YKL093W 2 2 25.1% 339 36935 6.4 U MBR1 SGDID:S000001576, Chr XI from 264433-265452, Verified ORF, "Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants" * Jamie_Ashy_SPB_45.16084.16084.4 2.7487 0.2068 93.7 6262.8965 6259.912 6373.2 143 4.222 10.7 1 K.KKTCAGSFLEPISQRSSSSSRGNVLLGNINTNCKKLPNRMTTNNSSSSTFYSTTKAK.R * Jamie_Ashy_SPB_51.18304.18304.3 2.7413 0.0225 52.1 3270.9844 3270.5244 6773.0 282 3.021 19.4 1 K.CLPNLTQQCKANCPSSDPQFNFSSLKAR.L Reverse_YMR311C 2 2 24.9% 229 26649 4.7 U GLC8 SGDID:S000004928, Chr XIII from 897602-896913, reverse complement, Verified ORF, "Regulatory subunit of protein phosphatase 1 (Glc7p), involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2" * Jamie_Ashy_SPB_50.17995.17995.2 2.1115 0.2467 15.0 2462.8523 2465.6758 7347.7 1 5.124 32.5 1 D.NTEFDPQKNEKIEFEPEGLSL.D * Jamie_Ashy_SPB_68.24514.24514.4 3.3549 0.1309 10.6 4207.9365 4213.307 8130.0 2 3.603 21.0 1 A.VQCPKKDSNDEDEDDDVRYYEGHPDVAGQYPTKPED.I Reverse_YNR007C 2 2 24.2% 310 35887 4.7 U ATG3 SGDID:S000005290, Chr XIV from 640116-639184, reverse complement, Verified ORF, "Protein involved in autophagy; E2-like enzyme that plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy" * Jamie_Ashy_SPB_103.36951.36951.3 1.4811 0.0148 33.2 4764.6543 4763.698 5250.1 5 3.528 10.0 1 R.QRVVRVKDLLIKMVNAHKCPHISVSLVSNKYFPLKEITATK.T * Jamie_Ashy_SPB_83.29784.29784.4 3.1769 0.0919 17.6 3834.9766 3832.2478 5708.1 284 3.261 16.7 1 R.YDASIDEFMQEPSLPSGNSNFGVIYMKPVRYSTS.Y YLR074C 2 2 24.1% 166 18517 8.9 U BUD20 SGDID:S000004064, Chr XII from 282457-281957, reverse complement, Verified ORF, "Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern" * Jamie_Ashy_SPB_106.37924.37924.2 1.7215 0.0937 14.6 2093.7322 2095.3545 4219.9 459 3.494 26.5 1 R.DLDLIYNDLSTKESVQKL.L * Jamie_Ashy_SPB_38.13604.13604.3 2.3146 0.1919 13.0 2036.0643 2040.0588 6145.8 299 4.264 23.8 1 A.AADAEANTAAVTEAESTASAST.- contaminant_KERATIN22 10 12 24.0% 645 65865 8.0 U no description * Jamie_Ashy_SPB_32.11455.11455.2 2.5215 0.246 100.0 1839.5322 1840.0055 7019.0 1 5.533 38.1 3 K.SISISVAGGGGGFGAAGGFGGR.G Jamie_Ashy_SPB_17.05989.05989.1 1.78 0.2137 100.0 1476.17 1476.6726 6479.7 2 4.401 59.1 1 R.FLEQQNQVLQTK.W * Jamie_Ashy_SPB_18.06217.06217.2 2.4304 0.302 100.0 1037.6322 1038.1454 5393.1 6 6.189 75.0 1 R.YLDGLTAER.T Jamie_Ashy_SPB_5.01832.01832.2 2.1983 0.1982 81.2 1194.6921 1195.3176 4523.2 20 4.61 75.0 1 K.KYEDEINKR.T * Jamie_Ashy_SPB_27.09613.09613.4 4.5001 0.3724 100.0 1983.1365 1982.2444 3923.4 2 6.068 35.4 1 R.HGDSLKEIKIEISELNR.V * Jamie_Ashy_SPB_27.09696.09696.3 3.9898 0.2697 100.0 1984.4644 1982.2444 6762.9 1 5.473 43.8 1 R.HGDSLKEIKIEISELNR.V * Jamie_Ashy_SPB_3.00826.00826.1 1.6716 0.1524 98.1 779.41 779.83075 4227.9 58 4.81 56.2 1 K.AAFGGSGGR.G * Jamie_Ashy_SPB_12.04282.04282.2 2.2328 0.0709 92.6 1741.1921 1742.716 5090.0 1 4.243 38.6 1 R.GGSGGGGSISGGGYGSGGGSGGR.Y * Jamie_Ashy_SPB_68.24325.24325.2 1.64 0.0799 13.6 2397.152 2397.4397 8880.1 448 3.731 19.6 1 G.SGGGSKGGSISGGGYGSGGGKHSSGGGSR.G * Jamie_Ashy_SPB_5.01548.01548.2 2.7907 0.3559 100.0 2006.2322 2006.9481 6339.7 1 5.808 33.3 1 R.GGSSSGGGYGSGGGGSSSVKGSSGE.A YPL172C 2 2 23.8% 462 52148 9.9 U COX10 SGDID:S000006093, Chr XVI from 225740-224352, reverse complement, Verified ORF, "Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders" * Jamie_Ashy_SPB_48.17114.17114.4 1.7141 0.0828 39.2 6920.3364 6919.101 4856.9 371 2.99 8.2 1 R.QMVRTQARPVVRGDVTPTQAFEFAALIGTLGVSILYFGVNPTVAILGASNIALYGWAYTSMKRK.H * Jamie_Ashy_SPB_97.34736.34736.4 2.6123 0.1611 14.3 5060.8164 5058.888 8791.1 5 3.96 13.7 1 G.ALVGMVPPLMGWAAASPLSHPGSWCLAGLLFAWQFPHFNTLSHNIR.N Reverse_YKL196C 2 2 23.0% 200 22707 5.7 U YKT6 SGDID:S000001679, Chr XI from 75539-74937, reverse complement, Verified ORF, "Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and within the Golgi, endocytic trafficking to the vacuole, and vacuolar fusion; membrane localization due to prenylation at the carboxy-terminus" * Jamie_Ashy_SPB_118.42438.42438.2 1.673 0.1058 15.6 1495.1921 1495.6904 9848.4 181 3.494 33.3 1 V.KMIADAQSPDQYK.S * Jamie_Ashy_SPB_76.27056.27056.4 3.1156 0.0915 19.5 3788.2966 3784.2727 6834.5 254 3.269 18.2 1 K.NLLTYAPRVPYEKDTILVGCIGESRAYVHGIYN.G YEL066W 2 2 22.9% 179 20699 7.6 U HPA3 SGDID:S000000792, Chr V from 26667-27206, Verified ORF, "D-Amino acid N-acetyltransferase, catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; similar to Hpa2p, acetylates histones weakly in vitro" * Jamie_Ashy_SPB_58.20653.20653.2 2.0636 0.0216 11.7 2076.912 2076.3906 7153.8 260 3.567 29.4 1 V.GMSNEEPEKMVNDRIVVK.A * Jamie_Ashy_SPB_78.27931.27931.2 1.3244 0.1769 15.8 2792.2522 2793.2847 9506.5 100 3.592 22.7 1 H.RAQLLYTKVAYKTDKVLYKRNGY.- contaminant_KERATIN19 3 3 22.6% 468 51203 5.5 U no description * Jamie_Ashy_SPB_68.24414.24414.2 1.4809 0.1132 11.8 1757.0122 1759.0183 5951.3 187 3.518 29.4 1 R.VAVRSAYGGPVGAGIREV.T * Jamie_Ashy_SPB_69.24795.24795.4 3.5514 0.1705 47.0 4469.6567 4469.097 8431.4 264 4.038 16.2 1 R.QEESEQIKALNNKFASFIDKVRFLEQQNKLLETKWTL.L * Jamie_Ashy_SPB_46.16248.16248.4 2.5013 0.1834 12.6 4744.2163 4748.3374 6895.3 18 3.665 13.0 1 G.VGAVNISVMNSTGGSSSGGGIGLTLGGTMGSNALSFSSSAGPGLLKAYSIR.T YKL180W 2 2 21.7% 184 20549 10.9 U RPL17A SGDID:S000001663, Chr XI from 109274-109582,109889-110134, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similarity to E. coli L22 and rat L17 ribosomal proteins; copurifies with the components of the outer kinetochore DASH complex" * Jamie_Ashy_SPB_39.13765.13765.2 1.6633 0.0836 12.8 2907.3323 2905.2917 5912.2 56 3.569 23.9 1 K.YLEQVLDHQRAIPFRRFNSSIGRT.A Jamie_Ashy_SPB_26.09316.09316.2 2.0022 0.114 94.2 1646.0721 1645.8558 6955.6 11 3.674 46.7 1 K.FVQGLLQNAAANAEAK.G Reverse_YDL013W 4 4 21.2% 619 71071 6.7 U HEX3 SGDID:S000002171, Chr IV from 429064-430923, Verified ORF, "Ring finger protein involved in the DNA damage response with possible recombination role; genetically identified by synthetic lethality with SGS1 (DNA helicase) and TOP3 (DNA topoisomerase); sporulation role; interacts with Slx8p and Lin1p" * Jamie_Ashy_SPB_52.18627.18627.4 2.5928 0.115 79.2 5980.5767 5979.622 8136.2 2 3.426 12.8 1 R.ALTQYPCHFKYKSVLGEFSVGRDKQSIGTFDDPIGVGLEAGCLCCVPIDELTK.A * Jamie_Ashy_SPB_19.06878.06878.3 3.064 0.0811 19.1 2233.3145 2233.4778 5112.4 210 3.701 27.6 1 D.ELTKALDDDTVIITEEKGNK.T * Jamie_Ashy_SPB_90.32155.32155.4 2.5529 0.0774 67.2 4923.4565 4927.4966 8078.3 20 3.357 15.4 1 K.NMLNKMVVDREREEREQIMDIINQTHREEDADSLAPGSWLR.S * Jamie_Ashy_SPB_16.05709.05709.3 2.7912 0.0724 76.1 2575.7344 2574.9053 7014.7 3 3.473 27.5 1 R.HFASRIDPTYSQIRERISQLR.I YBR057C 4 4 21.0% 366 41433 5.9 U MUM2 SGDID:S000000261, Chr II from 353291-352191, reverse complement, Verified ORF, "Cytoplasmic protein essential for meiotic DNA replication and sporulation; interacts with Orc2p, which is a component of the origin recognition complex" * Jamie_Ashy_SPB_63.22647.22647.4 2.8852 0.1416 13.8 6346.2964 6351.018 8717.9 321 3.366 10.6 1 V.NSNEMNSNKDDRMEALEVELQIKESQIESLENEIQRLKKIFNEGLNYKQNEHK.Y * Jamie_Ashy_SPB_23.08050.08050.3 3.1242 0.1483 25.0 2188.1343 2186.4705 7062.0 289 3.942 29.4 1 L.QIKESQIESLENEIQRLK.K * Jamie_Ashy_SPB_6.01951.01951.2 2.4226 0.0696 31.4 1359.9922 1359.4789 6093.4 15 3.511 70.0 1 S.QIESLENEIQR.L * Jamie_Ashy_SPB_81.29136.29136.4 2.4636 0.1369 15.0 2632.5764 2626.9268 4274.2 113 3.325 26.1 1 E.LREKIASLEAHLASKESSKEDVAN.- YKR085C 2 2 21.0% 195 22392 10.2 U MRPL20 SGDID:S000001793, Chr XI from 599323-598736, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the large subunit" * Jamie_Ashy_SPB_42.14902.14902.2 1.8096 0.0962 14.4 1441.2322 1440.6824 5272.5 1 3.446 59.1 1 G.FPKTQVTTIYNK.T * Jamie_Ashy_SPB_21.07256.07256.3 2.5389 0.1775 14.3 3174.4143 3170.6672 6423.0 74 3.978 19.6 1 P.RRGLDMPVQSTKAKQCPNVLVGKSTVNGK.T YKR053C 3 3 20.5% 404 46488 7.7 U YSR3 SGDID:S000001761, Chr XI from 534923-533709, reverse complement, Verified ORF, "Dihydrosphingosine 1-phosphate phosphatase, membrane protein involved in sphingolipid metabolism; has similarity to Lcb3p" * Jamie_Ashy_SPB_21.07569.07569.2 1.8582 0.0333 10.5 1645.8522 1647.8633 5675.2 163 3.191 35.7 1 I.IQTVTELGVTEDTIK.V * Jamie_Ashy_SPB_76.27136.27136.4 2.9554 0.1902 10.0 4627.6167 4633.281 7810.7 1 4.034 17.5 1 G.EHLWFPLLSVAWGLFILFNHVRPIDECPCFEDSVAFIGV.V * Jamie_Ashy_SPB_38.13660.13660.4 3.2601 0.0742 15.7 3423.2566 3428.6377 8038.3 390 3.628 18.5 1 V.VSGLDCSDWLTERYGWNLVCSRYASCGSK.V Reverse_YDL144C 2 2 20.5% 356 39783 6.7 U YDL144C SGDID:S000002303, Chr IV from 199733-198663, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_48.17070.17070.3 2.6587 0.0761 71.9 3135.5344 3136.6646 6167.3 1 3.054 23.1 1 R.IPNGLIVELEMLQGKECDVCMSPKFVK.K * Jamie_Ashy_SPB_92.32995.32995.4 2.3319 0.2161 11.5 5052.1367 5054.591 7109.0 7 4.169 14.1 1 V.PRIINSVRSNVPGDIINKTTVVIYNYGQNDAASAADEVSSYIHHPR.W YDR312W 2 2 20.3% 453 51856 9.0 U SSF2 SGDID:S000002720, Chr IV from 1087576-1088937, Verified ORF, "Protein required for ribosomal large subunit maturation, functionally redundant with Ssf1p; member of the Brix family" * Jamie_Ashy_SPB_42.14973.14973.4 2.114 0.0054 35.1 5421.7764 5416.328 6709.8 36 2.941 13.6 1 R.TSLNSIKRIFMINKDRETGEISMRHYFIDIREVEISRNLKRLYK.A * Jamie_Ashy_SPB_81.29148.29148.4 2.5518 0.165 88.1 5525.177 5523.1636 9320.6 3 4.361 14.2 1 K.TPVKMTDNEEREKGIEEEDVEMEEPKPSENSQPTPRKKAIKLTELGPR.L Reverse_YEL009C 2 2 20.3% 281 31310 5.2 U GCN4 SGDID:S000000735, Chr V from 139763-138918, reverse complement, Verified ORF, "Transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels" * Jamie_Ashy_SPB_23.08000.08000.2 1.6742 0.0649 13.0 1228.4521 1227.4001 3539.7 180 2.792 40.9 1 R.KLAAPDSSEPVI.P * Jamie_Ashy_SPB_64.22954.22954.4 3.2039 0.1613 21.5 4746.0566 4747.2124 6909.3 42 3.697 13.6 1 K.ADELVPTPLFSTTSVELNSPVLSVEETSEIAKDALSVDDTTVPID.N Reverse_contaminant_SPH_STRPY 2 2 20.2% 376 42501 9.0 U owl|P50470| IMMUNOGLOBULIN G BINDING PROTEIN H PRECURSOR (PROTEIN H). - STREPTOCOCCUS... * Jamie_Ashy_SPB_74.26448.26448.3 2.8597 0.1783 10.8 2999.4543 2998.3635 7423.7 9 4.116 21.3 1 T.DPTQSDSAKGARLKALEEAQKALQEKLA.K * Jamie_Ashy_SPB_64.22832.22832.4 2.5549 0.1298 14.9 5106.0166 5110.7734 6146.0 8 3.713 14.2 1 L.KANDEDLKKYEEQWDIKAGEAKVTTQNAFGAGLVTLAVAVSATGTKLK.R Reverse_YMR017W 3 3 19.9% 397 46622 9.2 U SPO20 SGDID:S000004619, Chr XIII from 307488-308681, Verified ORF, "Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog" * Jamie_Ashy_SPB_39.13936.13936.2 1.3677 0.0389 63.4 3100.892 3103.6365 9438.2 181 3.444 19.2 1 R.AEIAKELTRSTTKVSKLKTSRIESRLR.D * Jamie_Ashy_SPB_21.07592.07592.4 2.5696 0.1331 15.0 3611.4565 3616.9238 8434.5 311 3.211 17.2 1 R.NSKPFVEELSITALGDREPVEYSVCKDDSTKT.A * Jamie_Ashy_SPB_57.20208.20208.2 1.6705 0.0656 75.5 2327.2322 2326.6572 8922.2 297 3.294 26.3 1 R.FPSLYKTEHSGTINSILVYR.H YKL040C 3 3 19.9% 256 29174 5.0 U NFU1 SGDID:S000001523, Chr XI from 361471-360701, reverse complement, Verified ORF, "Protein involved in iron metabolism in mitochondria; similar to NifU, which is a protein required for the maturation of the Fe/S clusters of nitrogenase in nitrogen-fixing bacteria" * Jamie_Ashy_SPB_66.23502.23502.3 2.7357 0.12 17.2 2942.6343 2941.283 7486.9 7 3.17 22.1 1 K.TLTTPNENALKFLSTDGEMLQTRGSKS.I * Jamie_Ashy_SPB_27.09507.09507.2 1.7606 0.1209 19.6 1184.8922 1182.4639 5013.5 1 3.752 77.8 1 T.LKYGIESMLK.H * Jamie_Ashy_SPB_92.33012.33012.2 1.6622 0.0746 12.9 1629.6721 1627.9366 5535.5 3 3.616 42.3 1 E.VIQIMDPEQEIALK.E contaminant_NRL_1MCOH 3 3 19.4% 428 46852 8.9 U owl|| Immunoglobulin g1 (igg1) (mcg) with a hinge deletion, chain H... * Jamie_Ashy_SPB_29.10158.10158.4 3.2604 0.1194 10.9 3696.6565 3697.2817 5691.8 170 3.557 16.1 1 V.LHQNWLDGKEYKCKVSNKALPAPIEKTISKAK.G * Jamie_Ashy_SPB_57.20407.20407.4 3.7678 0.1684 58.1 4444.0166 4438.021 8290.7 110 4.305 15.4 1 K.GQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAV.E * Jamie_Ashy_SPB_4.01479.01479.2 2.135 0.0339 16.0 1511.7922 1509.6824 5622.5 1 3.305 68.2 1 V.MHEALHNHYTQK.S Reverse_YNR054C 2 2 19.3% 316 36408 8.0 U ESF2 SGDID:S000005337, Chr XIV from 724307-723357, reverse complement, Verified ORF, "Essential nucleolar protein involved in pre-18S rRNA processing; component of the small subunit (SSU) processome; has sequence similarity to mABT1, a mouse transcription activator" * Jamie_Ashy_SPB_87.31292.31292.3 2.5428 0.0361 50.4 3461.4844 3462.897 7594.9 153 3.687 18.3 1 K.VNLIDDHYFTGKKGGIINGNLTEACLKADRK.R * Jamie_Ashy_SPB_59.21279.21279.2 1.3946 0.0853 68.8 3377.4722 3375.848 10365.9 221 3.57 15.5 1 K.KAAKSKVLQRLKETKLDLSTTSSAQEEVER.D YFR028C 5 5 19.2% 551 61907 8.0 U CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit" * Jamie_Ashy_SPB_34.12070.12070.3 3.4518 0.1405 96.0 1973.3043 1972.2078 5907.2 7 3.597 42.2 1 R.FAVIFHEILNDPENANK.A * Jamie_Ashy_SPB_39.13788.13788.4 3.1447 0.0728 12.0 3719.8164 3716.1965 7666.4 38 3.446 18.3 1 K.SSHLNQPFKSVLNFFANNNVQLVVRLNSHLYN.K * Jamie_Ashy_SPB_13.04399.04399.2 2.5386 0.2872 100.0 2652.912 2652.7598 3956.0 35 5.203 28.3 1 R.KGQNGSNTIEDINNNRNPTSHANR.K * Jamie_Ashy_SPB_13.04480.04480.4 4.0746 0.2484 100.0 2656.0166 2652.7598 6620.3 1 4.91 29.0 1 R.KGQNGSNTIEDINNNRNPTSHANR.K * Jamie_Ashy_SPB_20.07231.07231.3 5.0275 0.2851 100.0 3566.8145 3566.7925 9579.4 1 6.079 30.5 1 R.KKNDISSASSSRMEDNEPSATNINNAADDTILR.Q Reverse_YNL292W 3 3 19.1% 403 45273 6.6 U PUS4 SGDID:S000005236, Chr XIV from 82806-84017, Verified ORF, "Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)" * Jamie_Ashy_SPB_10.03432.03432.2 1.9857 0.0406 14.5 1031.8121 1031.181 2987.9 2 3.473 78.6 1 L.KVMYCSSR.M * Jamie_Ashy_SPB_97.34701.34701.4 2.9161 0.1049 17.0 3481.8164 3478.875 4964.8 135 3.374 20.3 1 S.LIEGEVDGSTTSVGFLAESEYVKVTGSLYNALK.K * Jamie_Ashy_SPB_84.30170.30170.4 3.0411 0.0801 10.8 4194.5767 4195.859 6099.8 1 2.964 18.6 1 R.LKRKSAKKGTQKEYQQKREATARQIEKSFVQSKTLA.H Reverse_YPL170W 2 2 19.1% 152 16758 4.9 U DAP1 SGDID:S000006091, Chr XVI from 228313-228771, Verified ORF, "Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis" * Jamie_Ashy_SPB_82.29337.29337.2 1.7181 0.1027 16.1 2177.3123 2180.2036 9019.0 13 3.577 31.0 1 K.NSDNTNSAGNGTLGTPDESTKV.G * Jamie_Ashy_SPB_21.07351.07351.2 1.7485 0.0918 15.1 2050.0122 2049.2883 4691.3 324 3.874 28.9 1 G.NGTLGTPDESTKVGGFLLNK.I YDL044C 3 3 18.9% 440 51192 6.7 U MTF2 SGDID:S000002202, Chr IV from 375286-373964, reverse complement, Verified ORF, "Mitochondrial matrix protein that interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription" * Jamie_Ashy_SPB_18.06418.06418.3 2.5859 0.1473 16.6 2692.1643 2688.0337 5636.1 2 3.857 27.2 1 F.GELFEAGARVENMRHTNASKIIDK.Y * Jamie_Ashy_SPB_64.22777.22777.4 2.3905 0.0254 43.1 5856.2964 5857.5654 7068.5 438 2.495 11.9 1 R.HTNASKIIDKYYNGLQDNSEGTSVKKEKIVFNHSQRAQRKLPNKDHEFLK.E * Jamie_Ashy_SPB_15.05318.05318.2 1.582 0.0937 12.5 2266.0522 2266.4697 6307.6 4 3.584 38.9 1 R.TLLEKIFDEDNSINKSNRE.L Reverse_YBR143C 3 3 18.8% 437 49006 5.0 U SUP45 SGDID:S000000347, Chr II from 532176-530863, reverse complement, Verified ORF, "Polypeptide release factor involved in translation termination; mutant form acts as a recessive omnipotent suppressor" * Jamie_Ashy_SPB_104.37189.37189.4 3.0393 0.1565 11.2 3920.2566 3922.3374 6607.9 442 4.029 16.2 1 G.TAKDIAFSKDKAEPEAFKIVENDEADKFTYRITE.L * Jamie_Ashy_SPB_38.13629.13629.2 1.8211 0.0813 16.9 1871.4722 1874.1008 9872.0 32 3.467 36.7 1 F.KDDAQLLESLVETHFK.N * Jamie_Ashy_SPB_63.22578.22578.3 2.9029 0.1997 28.7 3504.4143 3508.1316 7744.4 321 3.86 16.9 1 T.GYEDTLMKQYLPIQGKPPIVLSIMSTGNGRAK.E YGR192C 3 3 18.7% 332 35747 7.0 U TDH3 SGDID:S000003424, Chr VII from 883815-882817, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " * Jamie_Ashy_SPB_22.07874.07874.4 2.8267 0.147 13.2 4460.2563 4466.1724 7424.6 35 3.537 15.8 1 R.IGRLVMRIALSRPNVEVVALNDPFITNDYAAYMFKYDST.H Jamie_Ashy_SPB_8.02916.02916.2 1.5385 0.0497 69.9 1298.0922 1298.4008 6768.7 202 3.407 55.0 1 K.TVDGPSHKDWR.G Jamie_Ashy_SPB_23.08323.08323.2 2.4064 0.2152 83.3 1542.6522 1541.5731 6480.9 1 4.131 68.2 1 V.SWYDNEYGYSTR.V YKR022C 2 2 18.3% 322 36647 5.7 U YKR022C SGDID:S000001730, Chr XI from 482782-481814, reverse complement, Uncharacterized ORF, "Protein required for spliceosome disassembly, forms a complex with the DExD/H-box RNA helicase Prp43p, which is known to govern the release of lariat intron from the spliceosome" * Jamie_Ashy_SPB_42.14919.14919.3 2.0477 0.0448 39.2 4125.5645 4127.3896 4240.5 20 2.736 17.6 1 K.RDITFDGEQAIKEDNSHYEDLYHSKKNTNASTRNK.D * Jamie_Ashy_SPB_21.07371.07371.2 2.1447 0.0347 15.2 2789.4922 2791.0874 7859.0 15 3.254 28.3 1 R.TNEEWETQLLSKGNINKSNEKIIT.P contaminant_KERATIN09 2 2 18.2% 429 47927 5.5 U no description * Jamie_Ashy_SPB_31.10935.10935.3 2.7471 0.1975 17.3 3058.8245 3056.3188 6843.0 37 4.025 18.8 1 R.SLGSVQAPSYGARPVSSAASVYAGAGGSGSRIS.V * Jamie_Ashy_SPB_85.30604.30604.4 2.5609 0.1404 19.0 5150.7764 5146.6514 6919.0 307 3.938 13.3 1 K.IMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAA.E YOL140W 2 2 18.2% 423 46681 7.5 U ARG8 SGDID:S000005500, Chr XV from 58758-60029, Verified ORF, "Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine" * Jamie_Ashy_SPB_9.03022.03022.4 2.9071 0.2982 96.7 3179.7766 3179.5132 5012.9 51 4.896 19.5 1 K.NAKLYDDVNGKEYIDFTAGIAVTALGHANP.K * Jamie_Ashy_SPB_60.21513.21513.4 2.8036 0.1681 12.0 5387.0566 5381.4326 8667.2 74 3.848 13.0 1 Q.VSKKSDILQKRLREIQAKYPNQIKTIRGKGLMLGAEFVEPPTEVIKK.A Reverse_YIL142W 3 3 18.0% 527 57203 6.1 U CCT2 SGDID:S000001404, Chr IX from 83302-84885, Verified ORF, "Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo" * Jamie_Ashy_SPB_65.23143.23143.4 3.0153 0.1644 16.4 3559.1765 3557.143 8135.1 60 3.656 19.4 1 K.MKEREAKELQALKATSDVKFKTGFIKVKDTD.L * Jamie_Ashy_SPB_91.32562.32562.4 3.0012 0.1292 14.7 4193.2964 4193.8794 7407.4 205 3.424 15.8 1 L.FSDSLKGGLIKIIQIHELNTSGKLRLIANTALEAFHDK.D * Jamie_Ashy_SPB_65.23160.23160.2 1.3213 0.0059 60.7 2612.912 2614.02 6172.8 435 3.308 18.0 1 K.PGLTSKVLDGVAIAGVFASLRANEAR.E Reverse_YGL091C 2 2 18.0% 328 35253 4.6 U NBP35 SGDID:S000003059, Chr VII from 343045-342059, reverse complement, Verified ORF, "Essential nuclear protein, evolutionarily conserved putative ATPase; exhibits genetic interaction with CDC2 encoding the catalytic subunit of DNA polymerase delta" * Jamie_Ashy_SPB_52.18454.18454.4 3.274 0.0018 63.4 4151.4966 4156.5337 8201.7 1 3.056 16.2 1 -.VDGVADRLAEVVNLVASSAPSDPYNDLFSEGMDCSKGIR.P * Jamie_Ashy_SPB_18.06398.06398.2 1.6294 0.1315 16.0 1993.0922 1994.2987 9266.0 179 3.785 26.3 1 K.GVGGKGSLVLIKHEIGSLND.T YML037C 2 2 17.9% 340 38023 7.0 U YML037C SGDID:S000004501, Chr XIII from 205408-204386, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_71.25482.25482.4 3.794 0.1362 27.5 4250.4165 4244.8467 7358.6 7 3.99 17.6 1 K.KSDGDKTKKNKSKRKSFFGFWGHSGSKSGSKKKSEKPI.E * Jamie_Ashy_SPB_33.11883.11883.2 1.7485 0.0786 13.2 2485.912 2482.9666 6955.3 19 3.942 27.3 1 E.PSIASTPSLTLDSFIPLQPKKKI.- YOL144W 3 3 17.8% 484 56966 6.0 U NOP8 SGDID:S000005504, Chr XV from 53096-54550, Verified ORF, "Nucleolar protein required for 60S ribosomal subunit biogenesis" * Jamie_Ashy_SPB_62.22114.22114.4 3.0682 0.1573 15.7 5318.177 5315.018 6953.1 42 3.742 14.0 1 F.QFEKHNHFAFIDIRFNDEADFNKLRKSFNNVKFKGNILKVDEAK.P * Jamie_Ashy_SPB_58.20588.20588.3 2.3813 0.0194 42.2 3200.2144 3201.4302 6591.7 81 2.75 19.4 1 R.NLFNPNEAEPVSQFKLIEDSDNDIDHAK.D * Jamie_Ashy_SPB_17.06080.06080.2 2.0518 0.061 16.1 1866.8922 1867.9774 5871.5 78 3.179 42.3 1 N.WDEEFWANRGNWTR.D YNR048W 2 2 17.8% 393 44542 8.0 U YNR048W SGDID:S000005331, Chr XIV from 711632-712813, Verified ORF, "Protein proposed to interact with phospholipid translocases, shares similarity to Cdc50p" * Jamie_Ashy_SPB_56.20102.20102.2 1.5905 0.1651 10.3 3019.372 3019.7493 9021.8 256 4.431 17.9 1 Q.PILSPQSVLPLLILMACVFAPIGIGLVVS.T * Jamie_Ashy_SPB_59.20931.20931.4 3.2283 0.0338 74.3 4925.6567 4926.473 8350.7 317 3.354 14.6 1 K.TEYNASDIVPPPNWAKLFPNGYTDDNIPDLQNWEQFKIWMR.T YJR107W 2 2 17.7% 328 37261 6.1 U YJR107W SGDID:S000003868, Chr X from 627254-628240, Uncharacterized ORF, "Putative protein of unknown function; has sequence or structural similarity to lipases" * Jamie_Ashy_SPB_77.27652.27652.4 2.8716 0.1203 18.1 4881.2964 4879.5386 7520.5 10 3.837 16.3 1 S.GYLAVDHGKKVVILAFRGSTTRQDWFSDFEIYPVNYSPLCVK.E * Jamie_Ashy_SPB_86.30990.30990.2 1.6595 0.0791 12.6 1937.8922 1940.0704 7369.7 413 3.115 33.3 1 K.QWVDELFETDAIEKES.I YDR383C 2 2 17.6% 238 26976 4.6 U NKP1 SGDID:S000002791, Chr IV from 1240666-1239950, reverse complement, Verified ORF, "Non-essential kinetochore protein, subunit of the Ctf19 central kinetochore complex (Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-Nkp1p-Nkp2p-Ame1p-Mtw1p)" * Jamie_Ashy_SPB_63.22686.22686.3 2.2669 0.0203 39.3 3736.3743 3734.2458 8208.0 2 2.932 17.4 1 K.DLILKCQALQIGESKLSDILSQTNSINSLTTSIK.E * Jamie_Ashy_SPB_8.02670.02670.2 1.5553 0.0701 12.9 893.5122 893.8364 5098.2 120 2.922 78.6 1 K.EASEDDDI.S Reverse_YOL135C 2 2 17.6% 222 25585 6.9 U MED7 SGDID:S000005495, Chr XV from 70043-69375, reverse complement, Verified ORF, "Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation" * Jamie_Ashy_SPB_14.04788.04788.2 2.4488 0.0467 10.2 2127.4521 2126.3838 6706.2 54 3.152 34.4 1 S.TLKDHVQKCVQEIERIE.G * Jamie_Ashy_SPB_102.36586.36586.3 2.4175 0.0669 61.0 2719.2244 2715.0789 6594.7 230 3.643 23.8 1 K.LLKRLEQIKYQYNTSENETSKK.Y YNR017W 2 2 17.6% 222 23244 7.4 U MAS6 SGDID:S000005300, Chr XIV from 662915-663583, Verified ORF, "Essential protein of the mitochondrial inner membrane, component of the mitochondrial import system" * Jamie_Ashy_SPB_56.20071.20071.4 2.7815 0.035 59.0 2478.8965 2473.835 4320.6 81 3.038 24.7 1 R.GKHDTAGSIGAGALTGALFKSSKGLK.P * Jamie_Ashy_SPB_42.15012.15012.2 1.8015 0.0446 13.2 1616.7322 1617.9414 7764.4 16 2.925 45.8 1 C.AVWCSVKKRLLEK.- YOR380W 3 3 17.4% 546 61287 7.8 U RDR1 SGDID:S000005907, Chr XV from 1051286-1052926, Verified ORF, "Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins" * Jamie_Ashy_SPB_67.23875.23875.4 2.8421 0.1127 17.5 5936.7764 5936.771 7050.7 43 3.366 12.3 1 K.NVEKYWDGDVKTLEYGAVIAGVIALGSFFMGSLGHPREMDIVQYAKGILDDPTF.S * Jamie_Ashy_SPB_34.12218.12218.4 3.0932 0.0884 16.9 3525.8564 3523.038 7006.3 210 3.342 19.4 1 R.EMDIVQYAKGILDDPTFSRIPTVEQVSAWVL.R * Jamie_Ashy_SPB_51.18168.18168.4 3.5416 0.1244 11.2 3298.7366 3304.7014 6573.4 66 3.644 20.5 1 E.HTRRLFWCAWSINTILSYDYGRSSVTL.N Reverse_YBR180W 3 3 17.3% 572 63407 8.8 U DTR1 SGDID:S000000384, Chr II from 589736-591454, Verified ORF, "Multidrug resistance dityrosine transporter of the major facilitator superfamily, essential for spore wall synthesis, facilitates the translocation of bisformyl dityrosine through the prospore membrane" * Jamie_Ashy_SPB_70.25164.25164.4 3.2594 0.2337 67.4 3505.0566 3499.9485 6901.0 73 4.494 21.9 1 I.SSCLGLGTFCWGIGMANCLQLIIASSIAAGVNR.F * Jamie_Ashy_SPB_28.10110.10110.4 3.5956 0.1146 11.5 3741.3765 3739.4036 7366.3 199 3.82 15.7 1 G.MNPGMMFYAIAKGRHKPPVVDTVTGAGLSIVSSSAF.A * Jamie_Ashy_SPB_73.26084.26084.2 1.3049 0.1535 12.2 3132.2722 3133.635 7418.9 111 4.286 17.2 1 K.RGGFDALAGWFLPGVSFVAMFVSVTANITT.A YOR274W 3 3 16.6% 428 50237 9.0 U MOD5 SGDID:S000005800, Chr XV from 837671-838957, Verified ORF, "Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase, required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms" * Jamie_Ashy_SPB_75.27036.27036.4 2.8363 0.1158 19.8 4507.9365 4508.9487 8214.3 43 3.512 15.8 1 K.GDIYLLDATDLSQWDTNASQRAIAISNDFISNRPIKQERA.P * Jamie_Ashy_SPB_6.02204.02204.3 2.4142 0.2477 65.6 1455.0543 1453.6926 5628.0 10 4.15 45.0 1 K.YWKIHLGSRRH.K * Jamie_Ashy_SPB_52.18451.18451.2 1.6003 0.0806 77.4 2520.5122 2521.8386 8110.6 1 3.516 31.6 1 K.RNTRQADFEKWKINKKETVE.- Reverse_YGR123C 4 4 16.4% 513 57995 7.7 U PPT1 SGDID:S000003355, Chr VII from 738208-736667, reverse complement, Verified ORF, "Protein serine/threonine phosphatase with similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth" * Jamie_Ashy_SPB_77.27728.27728.2 1.9032 0.0366 13.1 2124.0122 2126.38 8488.7 340 3.455 26.2 1 R.GAQLIGHGPVVHIVGGLNGQSD.C * Jamie_Ashy_SPB_54.19188.19188.2 1.9787 0.0938 16.3 1760.3722 1761.9532 8637.4 7 3.283 42.9 1 G.QSDCYNPASFVTMLK.G * Jamie_Ashy_SPB_79.28153.28153.2 1.8036 0.1299 10.3 1972.2522 1975.1804 6962.4 265 3.773 27.8 1 D.SLTASPDSPLGGHMVLYDN.N * Jamie_Ashy_SPB_63.22568.22568.4 2.8563 0.1471 13.2 3737.5764 3734.1804 7530.2 313 3.43 18.9 1 R.NLFFNNPHLIKLCYFLLAVECSWSGRDVFDG.N Reverse_YHR024C 2 2 16.4% 482 53338 6.3 U MAS2 SGDID:S000001066, Chr VIII from 159185-157737, reverse complement, Verified ORF, "Larger subunit of the mitochondrial processing protease, essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins" * Jamie_Ashy_SPB_14.04993.04993.4 3.1328 0.1025 16.6 3904.1765 3898.4597 6787.9 5 3.543 18.6 1 D.IPLGEFGIQIHFLEPLNGFVPAPPICSEGGTYQAVK.K * Jamie_Ashy_SPB_10.03633.03633.4 1.3929 0.0306 33.4 4595.8965 4598.135 4534.3 298 3.015 6.3 1 K.LNRGEFRSGADIYLGLASFHGPTNSTAVKLGNALSSLKFNDTR.A YNL166C 2 2 16.3% 448 49694 4.9 U BNI5 SGDID:S000005110, Chr XIV from 323567-322221, reverse complement, Verified ORF, "Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner" * Jamie_Ashy_SPB_88.31699.31699.4 3.343 0.1474 17.9 4496.177 4499.9014 7579.8 43 3.996 14.6 1 R.VDKGTEENSEIESFKSPMPQNNTLGGENKLDAELVLDKFSS.A * Jamie_Ashy_SPB_51.18153.18153.4 3.4823 0.2071 63.6 3705.1765 3702.3281 6734.0 341 4.001 18.3 1 I.KLQKEIDYLNKMNAQGSLSMEDGKRLHRAVVK.L Reverse_YMR120C 4 4 16.2% 592 65263 6.6 U ADE17 SGDID:S000004727, Chr XIII from 509279-507501, reverse complement, Verified ORF, "Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade16p; ade16 ade17 mutants require adenine and histidine" * Jamie_Ashy_SPB_58.20586.20586.4 2.7312 0.12 81.9 3304.6165 3307.6384 7382.3 155 3.418 20.8 1 K.SQYESLEPEETPIQGTVFLDIANSKEPRK.V Jamie_Ashy_SPB_17.06153.06153.3 2.2839 0.1343 15.2 1750.4644 1748.9768 4243.1 19 3.692 41.1 1 S.VIEKFTSQNIIADNR.K * Jamie_Ashy_SPB_68.24399.24399.2 1.2906 0.0351 61.5 3052.6921 3051.551 9840.7 322 3.095 15.5 1 K.AAARLLTVGGIDIEEVAEPITVGVKAVTEK.F * Jamie_Ashy_SPB_44.15708.15708.2 1.9443 0.0787 15.0 2343.2922 2344.7837 6712.1 31 3.623 28.6 1 E.IPFGADRIMRATGGSALIRVNK.E YNL220W 3 3 16.2% 433 48279 8.1 U ADE12 SGDID:S000005164, Chr XIV from 234414-235715, Verified ORF, "Adenylosuccinate synthase, catalyzes the first committed step in the 'de novo' biosynthesis of adenosine" * Jamie_Ashy_SPB_21.07308.07308.4 2.7916 0.1343 12.9 2011.6965 2015.2708 5022.0 183 3.397 30.4 1 R.TIDEIYGVVKAYTTRVGE.G * Jamie_Ashy_SPB_49.17521.17521.4 2.7713 0.0137 50.8 4121.177 4117.7876 7185.7 446 2.507 16.2 1 K.RRCGWLDLVVLKYSTLINGYTSLNITKLDVLDTFK.E * Jamie_Ashy_SPB_74.26340.26340.2 1.5052 0.1793 18.7 2795.9922 2793.2732 8610.0 31 4.723 22.9 1 K.LDVLDTFKEIPVGISYSIQGKKLDL.F Reverse_YOL116W 2 2 16.2% 382 43060 6.7 U MSN1 SGDID:S000005476, Chr XV from 99808-100956, Verified ORF, "Transcriptional activator involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus" * Jamie_Ashy_SPB_12.04264.04264.2 1.0184 0.1171 63.2 2799.4521 2801.1462 8891.0 61 3.314 19.6 1 R.WATQYLAEMKRISPQGNVGETYEK.W * Jamie_Ashy_SPB_63.22552.22552.4 3.4769 0.1191 10.1 4199.0166 4204.4443 9322.0 17 3.787 17.6 1 E.QSNGNLIDEFLTENSVAGENQPHATQDNNMPVSTYNRP.A Reverse_YLR216C 3 3 16.2% 371 42072 6.2 U CPR6 SGDID:S000004206, Chr XII from 573213-572098, reverse complement, Verified ORF, "Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity" * Jamie_Ashy_SPB_75.26911.26911.4 2.6984 0.0016 44.5 3361.7766 3359.8474 5812.2 77 3.056 20.1 1 R.RYLAKAKAKEDAAEAYLVESSAVLVQKYDK.L * Jamie_Ashy_SPB_67.23904.23904.2 1.7492 0.1929 45.5 1432.2922 1430.5969 5431.0 46 4.143 50.0 1 K.ELDEPFYEKLF.K * Jamie_Ashy_SPB_22.07856.07856.3 2.0291 0.0337 39.1 2285.7544 2282.6445 6308.4 251 2.673 26.4 1 K.LFNEATKPVIDNYLEFVIR.G YGR062C 2 2 16.1% 316 35650 11.2 U COX18 SGDID:S000003294, Chr VII from 617282-616332, reverse complement, Verified ORF, "Mitochondrial inner membrane protein, required for export of the Cox2p C terminus from the mitochondrial matrix to the intermembrane space during its assembly into cytochrome c oxidase; similar to Oxa2p of N. crassa" * Jamie_Ashy_SPB_40.14124.14124.4 2.5193 0.0299 48.2 3223.6165 3227.007 6403.4 27 2.602 21.0 1 R.ILKQQELRKLVQPITPIIKLRLAAVTNK.K * Jamie_Ashy_SPB_70.25176.25176.3 2.1751 0.1854 18.0 2452.8542 2451.8438 5207.1 18 3.811 26.1 1 L.AVLNVELNGRLMFSSSLSSQGIK.T YPL237W 2 2 16.1% 285 31574 9.5 U SUI3 SGDID:S000006158, Chr XVI from 100496-101353, Verified ORF, "Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding" * Jamie_Ashy_SPB_26.09051.09051.2 1.6811 0.1685 24.1 2043.9321 2045.4448 6785.4 137 3.421 28.1 1 K.FRIPPPVCLRDGKKTIF.S * Jamie_Ashy_SPB_44.15830.15830.2 2.323 0.0144 17.1 3196.9922 3199.7207 9939.6 94 3.292 19.6 1 L.IQYLFAELGTSGSVDGQKRLVIKGKFQSK.Q Reverse_YOL154W 2 2 16.1% 249 27567 5.0 U ZPS1 SGDID:S000005514, Chr XV from 34657-35406, Verified ORF, "Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH" * Jamie_Ashy_SPB_40.14210.14210.2 1.6584 0.0959 15.2 1064.6322 1065.171 3842.1 8 3.673 62.5 1 V.DDVGYNLLR.D * Jamie_Ashy_SPB_60.21639.21639.3 2.8185 0.2366 52.6 3480.9543 3482.7078 6507.4 374 4.832 16.7 1 R.ANTENCTQYITPFTAHSWGLIEQSSPSQLEA.T Reverse_YLR180W 2 2 16.0% 382 41818 5.2 U SAM1 SGDID:S000004170, Chr XII from 515264-516412, Verified ORF, "S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)" Jamie_Ashy_SPB_73.26293.26293.2 1.7563 0.1059 15.8 2690.0522 2690.978 6944.7 231 3.673 22.0 1 K.AVWRAAYAASRDVKSYDKGSFAGGGV.S * Jamie_Ashy_SPB_102.36697.36697.4 2.8441 0.1194 17.8 3942.5764 3948.2983 6901.9 292 3.669 15.7 1 A.GIDELDKEEHVGQAIDPSQQEIAVLVNCTKYDFGK.A YLR457C 3 3 16.0% 319 37354 10.2 U NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition" * Jamie_Ashy_SPB_1.00350.00350.2 2.8301 0.2287 100.0 1503.2722 1503.7422 6569.1 1 4.296 63.6 1 R.KVTDRKTEEKIR.T * Jamie_Ashy_SPB_15.05308.05308.1 2.1811 0.3239 100.0 1399.44 1400.4596 4562.5 29 6.107 45.5 1 R.DVFSNDNENMSK.M * Jamie_Ashy_SPB_47.16957.16957.4 3.4674 0.0476 12.1 3135.3765 3129.5645 6178.8 39 3.577 19.9 1 R.SQGRPSYMDTDTAKSRILRSDAFKRKI.S YGL076C 2 2 16.0% 244 27638 10.1 U RPL7A SGDID:S000003044, Chr VII from 365999-365989,365529-365436,364967-364338, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl7Bp and has similarity to E. coli L30 and rat L7 ribosomal proteins; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2)" * Jamie_Ashy_SPB_23.08186.08186.2 1.8743 0.0825 87.1 1771.1322 1773.987 6155.3 192 2.95 33.3 1 K.KSKAQQKTAEQVAAER.A Jamie_Ashy_SPB_82.29427.29427.2 1.3234 0.0227 61.9 2723.4722 2725.1685 9422.1 32 3.383 22.7 1 R.KFKHFIQGGSFGNREEFINKLVK.S Reverse_contaminant_KERATIN03 3 3 15.7% 593 59519 5.2 U no description * Jamie_Ashy_SPB_31.11146.11146.4 3.829 0.1782 64.5 3954.2966 3952.8057 6547.1 2 4.409 15.3 1 G.GYGGGSSGGGSSGGGYGGGSSGGHGGGYGGGSSGGGSSGGGYGGGFSGGGR.G * Jamie_Ashy_SPB_97.34646.34646.2 1.8345 0.1275 10.9 2277.7322 2279.5212 8539.7 197 3.758 26.5 1 E.NELRIKIDLLQQYETNQC.E * Jamie_Ashy_SPB_48.17224.17224.2 1.8251 0.095 14.9 2037.1122 2040.0392 7355.7 45 3.438 28.3 1 S.SVGGGGGCGGGGGGGGSRSSSYHK.S YFL034C-B 2 2 15.7% 287 33276 9.6 U MOB2 SGDID:S000001859, Chr VI from 63992-63973,63858-63015, reverse complement, Verified ORF, "Component of the RAM signaling network, localizes and activates the Ace2p in the daughter cell nucleus to direct daughter cell-specific transcription of several genes involved in cell separation; Mob1p-like protein" * Jamie_Ashy_SPB_47.16713.16713.2 1.58 0.1498 17.1 2376.172 2374.7422 6239.4 238 4.011 22.5 1 R.QVSLPASQYIDLALTWINNKV.N * Jamie_Ashy_SPB_108.38649.38649.3 2.1127 0.0351 39.4 2950.8542 2947.3262 4306.3 179 2.962 21.9 1 K.VNDKNLFPTKNGLPFPQQFSRDVQR.I YPL131W 4 4 15.5% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_Ashy_SPB_36.12726.12726.2 3.2588 0.1855 100.0 2209.632 2208.297 7067.6 2 4.793 42.1 1 K.GVEEVEGEYELTEAVEDGPR.P * Jamie_Ashy_SPB_39.13923.13923.2 2.975 0.1713 100.0 2582.0723 2580.7644 7804.0 1 4.944 40.9 1 K.GVEEVEGEYELTEAVEDGPRPFK.V * Jamie_Ashy_SPB_23.08161.08161.3 2.7383 0.1117 15.4 2744.7844 2747.9092 7296.2 214 3.293 20.5 1 L.RSYIFGGHVSQYMEELADDDEER.F * Jamie_Ashy_SPB_23.08161.08161.2 3.2885 0.4056 100.0 1830.1921 1829.8867 7296.2 1 6.424 57.1 1 H.VSQYMEELADDDEER.F Reverse_YBR146W 2 2 15.5% 278 31925 10.3 U MRPS9 SGDID:S000000350, Chr II from 535254-536090, Verified ORF, "Mitochondrial ribosomal protein of the small subunit" * Jamie_Ashy_SPB_113.40658.40658.4 2.7305 0.1265 18.5 3503.3364 3501.1277 4653.0 178 3.562 20.0 1 D.KMKLFYDNLQRGNVLIEGTGRVVFVKATSSK.R * Jamie_Ashy_SPB_4.01298.01298.3 2.6021 0.0941 18.6 1559.4243 1560.8113 5789.7 42 3.516 40.9 1 D.ETKQYITRFSMR.M YJL104W 2 2 15.4% 149 16216 9.4 U PAM16 SGDID:S000003640, Chr X from 227244-227693, Verified ORF, "Constituent of the mitochondrial import motor associated with the presequence translocase, along with Ssc1p, Tim44p, Mge1p, and Pam18p; has similarity to J-domain containing proteins" * Jamie_Ashy_SPB_3.01069.01069.2 1.7272 0.0352 10.1 1245.2122 1243.3221 5365.0 28 2.957 55.0 1 E.YGGITLDESCK.I * Jamie_Ashy_SPB_7.02349.02349.2 1.8754 0.0191 10.6 1126.2922 1125.0508 5274.6 184 3.445 50.0 1 N.SSGADNSASSNQ.- YDL159W 2 2 15.3% 515 57709 9.2 U STE7 SGDID:S000002318, Chr IV from 172482-174029, Verified ORF, "Signal transducing MAP kinase kinase involved in pheromone response, where it phosphorylates Fus3p, and in the pseudohyphal/invasive growth pathway, through phosphorylation of Kss1p; phosphorylated by Ste11p, degraded by ubiquitin pathway" * Jamie_Ashy_SPB_14.04806.04806.4 2.9811 0.139 12.9 3799.3765 3797.3518 6664.9 280 3.304 17.2 1 V.QRGTVSSKKTWFNELTISKIAYGVLNGLDHLYR.Q * Jamie_Ashy_SPB_110.39394.39394.4 1.4754 0.0013 33.4 5882.2563 5876.5454 5136.4 225 2.711 7.8 1 K.EMTDFVNRCCIKNERERSSIHELLHHDLIMKYVSPSKDDKFRHWCR.K Reverse_Q0160 2 2 15.3% 235 27683 9.3 U SCEI SGDID:S000007279, Chr Mito from 61022-61729, Verified ORF, "I-SceI DNA endonuclease, encoded by the mitochondrial group I intron of the 21S_rRNA gene; mediates gene conversion that propagates the intron into intron-less copies of the 21S_rRNA gene" * Jamie_Ashy_SPB_107.38306.38306.2 1.1706 0.1675 12.7 2516.392 2513.7095 7602.6 32 3.925 23.7 1 K.WEFQMCYTKGEDRSRIYADG.L * Jamie_Ashy_SPB_47.16808.16808.3 1.717 0.0128 33.3 1820.3644 1817.1749 4784.2 102 2.558 30.0 1 K.SNPGLNMVQNKKINKM.- YBR181C 3 3 15.3% 236 26996 10.4 U RPS6B SGDID:S000000385, Chr II from 592769-592764,592411-591707, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps6Ap and has similarity to rat S6 ribosomal protein" YPL090C 3 3 15.3% 236 26996 10.4 U RPS6A SGDID:S000006011, Chr XVI from 378392-378387,377992-377288, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps6Bp and has similarity to rat S6 ribosomal protein" Jamie_Ashy_SPB_101.36105.36105.2 1.8498 0.136 20.3 2051.6921 2050.6409 7223.3 27 4.686 35.3 1 F.PMKQGVLLPTRIKLLLTK.N Jamie_Ashy_SPB_113.40615.40615.1 1.1471 0.0595 79.5 884.15 884.0665 5226.6 69 3.471 57.1 1 K.QGVLLPTR.I Jamie_Ashy_SPB_118.42248.42248.2 1.8141 0.1276 18.5 2028.8722 2030.2859 9831.0 24 4.175 29.4 1 V.KKGEQELEGLTDTTVPKR.L YLR028C 3 3 15.2% 591 65283 6.6 U ADE16 SGDID:S000004018, Chr XII from 201316-199541, reverse complement, Verified ORF, "Enzyme of 'de novo' purine biosynthesis containing both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities, isozyme of Ade17p; ade16 ade17 mutants require adenine and histidine" * Jamie_Ashy_SPB_57.20311.20311.2 1.9534 0.1062 20.6 1536.9722 1539.7288 6647.1 164 3.705 36.7 1 V.SPAGAAVGLPLSDVER.Q * Jamie_Ashy_SPB_82.29508.29508.3 2.6471 0.1847 13.5 2866.9443 2870.256 5326.9 37 3.725 22.1 1 A.RARGADRMSSFGDFIALSNIVDVATAK.I * Jamie_Ashy_SPB_80.28624.28624.4 2.0993 0.0138 35.8 5214.8164 5217.0713 8134.9 3 2.799 15.2 1 R.EVFGVTLQQKRNDAIINQSTFKEIVSKNKALTEQAVIDLTVATLVLK.Y YLR259C 2 2 15.2% 572 60752 5.3 U HSP60 SGDID:S000004249, Chr XII from 665004-663286, reverse complement, Verified ORF, "Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; similarity to groEL" * Jamie_Ashy_SPB_74.26473.26473.4 3.1299 0.0911 19.4 4757.2964 4762.4155 8417.2 76 3.735 14.8 1 Q.IKGSIDITTTNSYEKEKLQERLAKLSGGVAVIRVGGASEVEVGEK.K * Jamie_Ashy_SPB_67.23884.23884.4 2.913 0.1018 16.0 4498.0166 4500.019 8717.8 116 3.355 14.6 1 R.KAITRPAKQIIENAGEEGSVIIGKLIDEYGDDFAKGYDASKS.E Reverse_YER122C 3 3 15.2% 493 55094 8.2 U GLO3 SGDID:S000000924, Chr V from 404348-402867, reverse complement, Verified ORF, "ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Gcs1p" * Jamie_Ashy_SPB_110.39421.39421.4 1.3214 9.0E-4 32.6 5539.4565 5536.79 5782.6 17 2.87 7.2 1 K.EAGQEVVDRLMQLEDDASANFDIFGNNSNRGDFNGAGSGSSNIPNGFEDVEK.D * Jamie_Ashy_SPB_45.16082.16082.2 1.7173 0.0601 10.7 1700.7922 1702.9486 11564.9 19 3.277 35.7 1 F.LDEAQSKDVKKATLR.T * Jamie_Ashy_SPB_22.07653.07653.2 1.3786 0.0050 61.8 931.4322 929.0695 3087.4 277 2.89 57.1 1 K.HNGGLKFR.R Reverse_YLR220W 2 2 15.2% 322 34250 5.1 U CCC1 SGDID:S000004210, Chr XII from 576827-577795, Verified ORF, "Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation" * Jamie_Ashy_SPB_118.42324.42324.2 1.6187 0.0919 11.6 1625.3322 1626.909 10428.5 1 4.677 43.3 1 T.VKKSTSSGSGMSLKTK.V * Jamie_Ashy_SPB_45.15912.15912.3 2.8267 0.1825 10.4 3330.3542 3334.8406 8734.2 54 3.994 18.8 1 Y.PVLPVLGGLLYGGGITVASILERNEAPEDLGRG.Y Reverse_YHR159W 3 3 15.1% 504 56314 8.3 U YHR159W SGDID:S000001202, Chr VIII from 417552-419066, Uncharacterized ORF, "Protein of unknown function that localizes to the cytoplasm, potential Cdc28p substrate" * Jamie_Ashy_SPB_99.35415.35415.2 1.7373 0.063 11.9 1438.4722 1437.5522 5200.6 274 3.472 45.8 1 D.TLSNNGANDKQFK.T * Jamie_Ashy_SPB_70.25064.25064.4 3.0192 0.1773 23.9 3310.9766 3309.595 7769.3 20 3.913 22.6 1 K.FKRMNLSSDDGSDEAQGRNTIKHTSKKQE.H * Jamie_Ashy_SPB_87.31183.31183.4 2.8884 0.0909 10.5 3701.2966 3695.9756 6496.6 83 3.429 18.7 1 R.NDTIVDNDNEEEIGLLGAKVDSVFSWLSRSVSGQ.S YKR018C 3 3 15.0% 725 81754 5.2 U YKR018C SGDID:S000001726, Chr XI from 475543-473366, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus" * Jamie_Ashy_SPB_75.26991.26991.2 1.9582 0.0765 15.1 2031.1322 2031.4037 7344.6 88 3.705 30.6 1 T.IKAANKAKKLKITSGSEDK.E * Jamie_Ashy_SPB_41.14716.14716.4 2.8046 0.1465 14.8 4436.6567 4439.207 8845.8 298 3.178 14.2 1 D.LSQATIDEFVHSGVNLCFGILQVVISLLPPAIGAVLSVVGFR.G * Jamie_Ashy_SPB_40.14308.14308.4 2.2504 0.1668 15.3 5579.0166 5582.465 8033.5 70 3.773 12.4 1 K.TFKSKNLPLDRFILRKVEQFKAKKEELGVENPLDGIATSPVHEIAYFY.N YPL209C 2 2 15.0% 367 42946 9.7 U IPL1 SGDID:S000006130, Chr XVI from 157592-156489, reverse complement, Verified ORF, "Aurora kinase involved in regulating kinetochore-microtubule attachments, associates with Sli5p, which stimulates Ipl1p kinase activity and promotes its association with the mitotic spindle, potential Cdc28p substrate" * Jamie_Ashy_SPB_71.25464.25464.4 3.1765 0.0834 82.2 4343.8164 4338.926 6524.7 58 3.009 15.3 1 K.KTTTRPNTSRINKPWRISHSPQQRNPNSKIPSPVREK.L * Jamie_Ashy_SPB_77.27640.27640.2 1.2848 0.1585 13.1 2830.152 2831.3062 7833.1 26 3.498 21.7 1 P.SPVREKLNRLPVNNKKFLDMESSK.I YKR010C 4 4 14.9% 771 86366 8.0 U TOF2 SGDID:S000001718, Chr XI from 460882-458567, reverse complement, Verified ORF, "Nonessential mitochondrial protein of unknown function with sequence similarity to Net1p; identified as a topoisomerase I (Top1p) binding protein; displays synthetic genetic interactions with TOP1 and HPR1" * Jamie_Ashy_SPB_81.29162.29162.3 2.7064 0.1503 15.5 3100.2544 3104.341 6543.0 1 3.609 22.1 1 R.LNTPQSVSPMVQYNEDIITHNNSINNC.S * Jamie_Ashy_SPB_38.13392.13392.4 3.084 0.1224 15.0 2908.0166 2912.0593 6317.6 427 3.331 18.6 1 K.DHARAKEGVVTQGSDNNKENIPSSTQQ.Q * Jamie_Ashy_SPB_14.04776.04776.1 1.2009 0.1794 96.6 988.41 988.1289 4930.4 53 3.791 50.0 1 K.PTGETKVQK.R * Jamie_Ashy_SPB_99.35580.35580.4 2.1046 0.2455 10.1 5505.0566 5503.756 11050.2 106 4.434 11.1 1 V.KVTRSHSSPSSSSSMSLESSLDSSSSDDSDDDSDSRNVQVKKINFKTSHGPA.G YML016C 2 2 14.9% 692 77491 8.3 U PPZ1 SGDID:S000004478, Chr XIII from 241536-239458, reverse complement, Verified ORF, "Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance" * Jamie_Ashy_SPB_62.22327.22327.4 3.1197 0.2223 58.0 6395.0166 6397.7495 9205.0 191 3.75 11.0 1 A.LTRSNSHASSLHSRKSSFGSDGNTAYSTPLNSPGLSKLTDHSGEYFTSNSTSSLNHHSSR.D * Jamie_Ashy_SPB_102.36763.36763.4 1.8525 0.0628 39.1 4875.9365 4875.6323 7457.3 22 3.181 14.3 1 K.NNEILQICIKAREIFLSQPSLLELSPPVKIVGDVHGQYGDLLR.L YPL134C 2 2 14.8% 310 34206 9.9 U ODC1 SGDID:S000006055, Chr XVI from 299502-298570, reverse complement, Verified ORF, "Mitochondrial inner membrane transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism" * Jamie_Ashy_SPB_69.24771.24771.2 1.893 0.0925 16.2 2254.0322 2253.5188 8383.0 64 3.369 27.8 1 K.FSGNDTFQTFYKKIFPTPN.G * Jamie_Ashy_SPB_94.33716.33716.2 1.3434 0.1624 14.9 2809.4922 2808.1272 8252.7 147 4.052 19.2 1 K.TSTEKTRNDLIAGAIGGTVGCLLNTPF.D YOR391C 2 2 14.8% 237 25926 7.6 U HSP33 SGDID:S000005918, Chr XV from 1079254-1078541, reverse complement, Uncharacterized ORF, "Possible chaperone and cysteine protease with similarity to E. coli Hsp31 and S. cerevisiae Hsp32p, Hsp33p, and Sno4p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease" * Jamie_Ashy_SPB_37.13069.13069.2 1.8379 0.1035 14.0 2255.7122 2258.5823 4305.3 2 3.563 36.8 1 K.ALARIKTANEVNASDYKVFF.A Jamie_Ashy_SPB_39.13962.13962.2 1.5804 0.1006 12.2 1608.5721 1610.8479 6778.6 1 3.753 46.4 1 F.PLEGEIALGVDDILR.S Reverse_YAL035W 7 7 14.7% 1002 112268 5.8 U FUN12 SGDID:S000000033, Chr I from 76428-79436, Verified ORF, "GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2" * Jamie_Ashy_SPB_35.12585.12585.4 3.0643 0.1092 87.4 2938.0566 2934.4631 5156.5 381 3.151 20.1 1 K.VDFCLMVAYEPAKELMTSAKMVDRK.Y * Jamie_Ashy_SPB_105.37508.37508.4 2.6011 0.0155 46.5 3365.2166 3366.9507 4485.9 34 3.222 19.5 1 R.LLKISEITQQELGHMIDIVLIAINCLSSGR.S * Jamie_Ashy_SPB_118.42434.42434.3 2.8419 0.1036 18.6 2613.6843 2613.1257 7474.1 50 3.726 25.0 1 L.KKEEKQKRTLLKGEAKRKERK.A * Jamie_Ashy_SPB_20.06909.06909.2 1.7356 0.0051 64.9 1828.2322 1829.1996 5313.8 129 2.867 32.1 1 K.RKERKAKEKEKKAAK.A * Jamie_Ashy_SPB_30.10621.10621.4 3.1105 0.1408 15.9 2197.7366 2198.5278 5162.1 68 3.532 31.2 1 E.RELKEQEELQKKLELQR.R * Jamie_Ashy_SPB_22.07717.07717.2 2.02 0.0171 12.5 1703.1522 1703.9364 6503.0 70 3.245 34.4 1 E.GKSKQSKEAAAKEAAAK.E * Jamie_Ashy_SPB_12.04280.04280.3 3.1829 0.0229 68.0 3286.2544 3283.8025 8706.7 9 2.888 21.2 1 K.QAQQQAKKRAAQEKKKQKEKEKKEKEK.E Reverse_YDL108W 2 2 14.7% 306 35247 6.9 U KIN28 SGDID:S000002266, Chr IV from 267698-267725,267807-268699, Verified ORF, "Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters" * Jamie_Ashy_SPB_50.17827.17827.4 3.0266 0.0847 14.1 2861.2166 2863.3318 6233.4 357 3.165 22.7 1 E.AFIVGVSWIDIASTYHKAGFLLEPAR.Y * Jamie_Ashy_SPB_12.04291.04291.2 1.6996 0.1191 88.5 2026.3722 2027.2073 7049.0 121 3.809 30.6 1 R.GTSHQCGLYVVAYTGEGVK.K Reverse_YDL014W 2 2 14.7% 327 34465 10.2 U NOP1 SGDID:S000002172, Chr IV from 427361-428344, Verified ORF, "Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin" * Jamie_Ashy_SPB_88.31686.31686.3 1.7687 0.0619 35.8 4316.304 4317.7085 6909.0 1 3.071 14.9 1 R.HPEIVVKAGGRAGGAAGGRGGRSGGRAGGRSGGRGGFGGRSGGRAGGR.S * Jamie_Ashy_SPB_70.24927.24927.2 1.6516 0.071 11.2 2744.112 2744.9182 8803.9 140 4.212 17.7 1 A.GGRAGGAAGGRGGRSGGRAGGRSGGRGGFGGR.S Reverse_YDR527W 2 2 14.6% 439 50106 5.1 U RBA50 SGDID:S000002935, Chr IV from 1491084-1492403, Verified ORF, "Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1" * Jamie_Ashy_SPB_92.32893.32893.4 2.8856 0.1654 14.0 4506.3364 4508.094 5069.6 2 3.442 17.1 1 N.SISGDEKYTDADIEPVLQYYSKQGLKYLIRGLTQIAICR.Q * Jamie_Ashy_SPB_31.10902.10902.3 2.4873 0.0233 44.9 2756.9644 2755.9556 7008.2 14 2.896 24.0 1 K.INENHISKAESLPADTSTQQKEADK.G Reverse_YOL132W 3 3 14.4% 471 53813 4.9 U GAS4 SGDID:S000005492, Chr XV from 71299-72714, Verified ORF, "Putative 1,3-beta-glucanosyltransferase, has similarity to Gas1p; localizes to the cell wall" * Jamie_Ashy_SPB_84.30118.30118.2 1.4732 0.1022 10.7 2619.9321 2619.7095 11716.6 7 3.547 25.0 1 K.DEDADEQEEGELDALTLSQRIEI.K * Jamie_Ashy_SPB_14.04912.04912.2 2.0936 0.0045 14.7 1045.1721 1043.124 5360.8 3 3.015 68.8 1 V.YAPSYQASR.K * Jamie_Ashy_SPB_47.16627.16627.4 3.2935 0.147 16.3 4351.6963 4351.8643 7050.3 355 3.409 16.2 1 K.FVHELYVENYTTWPEYRNLHQNQLPSNVDLILYIGA.M YBR263W 2 2 14.3% 490 53686 8.7 U SHM1 SGDID:S000000467, Chr II from 736259-737731, Verified ORF, "Mitochondrial serine hydroxymethyltransferase, involved in one-carbon metabolism" * Jamie_Ashy_SPB_94.33766.33766.4 1.3177 0.0692 33.6 6644.0166 6648.325 7043.7 33 3.463 7.0 1 R.HRQKHSITLIPSENFTSKAVMDLLGSELQNKYSEGYPGERYYGGNEIIDKSESLCQAR.A * Jamie_Ashy_SPB_14.05073.05073.2 1.8628 0.1211 20.3 1227.9521 1225.3873 5240.2 163 3.504 45.5 1 K.LVSGGTDNHLIV.I Reverse_YMR316W 3 3 14.3% 336 38760 8.8 U DIA1 SGDID:S000004935, Chr XIII from 904823-905833, Verified ORF, "Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_Ashy_SPB_17.05944.05944.2 2.4605 0.0746 33.3 1455.9722 1457.6262 5558.6 35 3.542 58.3 1 G.VFSYTVNAQSVNK.Y * Jamie_Ashy_SPB_51.18186.18186.4 3.559 0.1438 24.2 3934.3765 3937.5364 6814.2 22 3.687 16.2 1 K.AVTYPPPEDATRLIFKFIKYYPVNGVVTNSHGRKG.F * Jamie_Ashy_SPB_66.23506.23506.2 1.4508 0.0332 64.4 1689.8522 1691.887 5490.8 366 3.167 35.7 1 K.YYPVNGVVTNSHGRK.G YDL114W 2 2 14.3% 308 34295 9.5 U YDL114W SGDID:S000002272, Chr IV from 255604-256530, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_73.26046.26046.4 2.9308 0.0901 12.2 3837.1365 3842.4727 6733.4 41 3.204 18.6 1 R.EGFIINIASVLGELTPARLTSYGASKGAMIGFHKCM.S * Jamie_Ashy_SPB_22.07617.07617.2 1.5057 0.1067 14.7 884.7922 884.9676 3686.5 29 3.921 64.3 1 D.HVTSTSPR.A Reverse_YBR187W 2 2 14.3% 280 30341 5.4 U YBR187W SGDID:S000000391, Chr II from 602629-603471, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YBR187W is an essential gene" * Jamie_Ashy_SPB_27.09445.09445.2 1.6875 0.0819 12.0 2168.112 2170.6462 8261.1 72 3.218 27.8 1 L.AFIFFLLSSALTITRISIR.T * Jamie_Ashy_SPB_70.25208.25208.2 2.0869 0.2579 23.5 2205.5122 2203.5483 7686.3 301 4.726 22.5 1 L.GIMSVSMLFSKLHSSSGSEMS.T Reverse_YGR012W 2 2 14.2% 393 42801 8.2 U YGR012W SGDID:S000003244, Chr VII from 513163-514344, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_76.27356.27356.4 2.9807 0.0904 14.7 3527.2166 3523.846 5526.3 21 3.303 20.0 1 I.EPGTTQYHVKWNAENEFQDAFVARIGNGSKK.L * Jamie_Ashy_SPB_91.32583.32583.4 2.524 0.034 49.3 2849.0166 2851.3623 4391.8 100 3.042 22.2 1 K.VLPTNGILEEVGEKPRPIDRTKFLK.Y YGR239C 2 2 14.2% 288 33050 6.7 U PEX21 SGDID:S000003471, Chr VII from 970058-969192, reverse complement, Verified ORF, "Part of a two-member peroxin family (Pex18p and Pex21p) specifically required for peroxisomal targeting of the Pex7p peroxisomal signal recognition factor and PTS2-type peroxisomal matrix proteins" * Jamie_Ashy_SPB_25.08992.08992.4 2.5485 0.1478 16.8 2279.1765 2281.4868 4893.6 238 3.696 28.9 1 T.KLAHTELTAHYATEDSHVEK.H * Jamie_Ashy_SPB_84.30246.30246.2 1.7181 0.1639 24.5 2082.632 2084.1646 6342.3 3 3.993 30.0 1 C.CTPPPSSASSSSTLSSSVESK.L YGR210C 2 2 14.1% 411 45214 8.2 U YGR210C SGDID:S000003442, Chr VII from 914743-913508, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * Jamie_Ashy_SPB_54.19310.19310.2 1.6195 0.0778 12.6 2900.0522 2901.348 6804.8 12 3.273 22.0 1 K.IDHPDADKNVSKIMLKYPDTKAVLTS.A * Jamie_Ashy_SPB_86.30912.30912.4 3.2643 0.1875 40.9 3373.8164 3369.843 6328.3 1 4.263 23.1 1 S.IETVGGVRVSEESLVEPGKNDILGFKIAPRSA.- Reverse_YOR305W 2 2 14.0% 242 27966 9.5 U YOR305W SGDID:S000005832, Chr XV from 889018-889746, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_41.14643.14643.2 1.475 0.0216 63.4 2364.9521 2364.626 7878.7 109 2.903 28.9 1 K.NRQSHSVLSTFTGVLERFER.P * Jamie_Ashy_SPB_93.33271.33271.2 1.7582 0.057 13.0 1578.1122 1578.7684 6376.4 43 3.607 42.3 1 K.LVFGNQSNTRKINS.A Reverse_YMR279C 2 2 13.9% 540 59562 8.2 U YMR279C SGDID:S000004892, Chr XIII from 826350-824728, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_101.36344.36344.4 2.2771 0.1653 15.9 3871.4565 3875.7761 6785.4 148 4.129 17.7 1 W.IGFSGWGLFVALLIMIMHRNKTMARPLLPVEAYK.S * Jamie_Ashy_SPB_85.30420.30420.4 2.7889 0.191 17.9 4645.2163 4642.2925 6466.3 39 3.839 15.0 1 A.QNNAESNFSKSLVNMICLAHTQGAQNLLQALMCTAIFMLEK.G YOL041C 2 2 13.9% 459 51942 9.4 U NOP12 SGDID:S000005401, Chr XV from 252644-251265, reverse complement, Verified ORF, "Nucleolar protein, required for pre-25S rRNA processing; contains an RNA recognition motif (RRM) and has similarity to Nop13p, Nsr1p, and putative orthologs in Drosophila and S. pombe" * Jamie_Ashy_SPB_32.11473.11473.4 2.2287 0.0286 40.9 5275.1367 5271.9604 6411.4 135 3.1 13.0 1 R.DTINAYIVYKNKSAVRKICSNLNAVVFQDHHLRVDSVAHPAPHDKK.R * Jamie_Ashy_SPB_46.16341.16341.3 2.5558 0.1835 11.0 1908.4443 1907.0476 6344.8 71 3.728 32.4 1 G.TTIGTGLDRNGLTDSQRT.R Reverse_YKL035W 2 2 13.8% 499 55988 7.4 U UGP1 SGDID:S000001518, Chr XI from 369534-371033, Verified ORF, "UDP-glucose pyrophosphorylase (UGPase), catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p" * Jamie_Ashy_SPB_27.09429.09429.2 2.157 0.0334 14.6 2061.5122 2062.377 6414.5 379 3.535 30.6 1 E.LLRVQGDYSILTGGKVDAR.T * Jamie_Ashy_SPB_84.30014.30014.4 2.1738 0.0208 38.7 5913.2163 5911.6514 8626.3 399 3.411 11.6 1 K.IKDWELTTRSSKEVLYRRFLTFFSDLENEFKARAADDLKSSDALKNLANR.M Reverse_YDR247W 2 2 13.7% 461 52136 6.9 U VHS1 SGDID:S000002655, Chr IV from 956007-957392, Verified ORF, "Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; homolog of Sks1p" * Jamie_Ashy_SPB_20.07147.07147.4 2.6682 0.042 63.1 2492.9766 2494.8682 4200.0 442 2.647 25.8 1 -.NTLSSSSMRLENFKKEFLSFK.N * Jamie_Ashy_SPB_68.24298.24298.4 3.0594 0.0851 15.4 4713.177 4715.4775 6546.0 138 3.361 14.2 1 V.PSVFKEYVSKSLPPVKSLPGENTFSTISSVEKMLEQLSMRNK.P Reverse_YHR097C 3 3 13.7% 366 40663 9.9 U YHR097C SGDID:S000001139, Chr VIII from 298612-298488,298363-297388, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_4.01153.01153.2 1.6408 0.0476 76.2 1128.8121 1129.3541 4158.0 21 3.179 62.5 1 K.KRAISNKRR.S * Jamie_Ashy_SPB_84.29928.29928.4 2.9625 0.1148 16.5 4544.5366 4548.951 6286.4 26 3.267 15.0 1 N.YNRPTEDRSSVRDESPSVRFPNIVTNVGNQPGGITRGTQNM.- * Jamie_Ashy_SPB_36.12747.12747.4 3.3219 0.0402 76.5 2987.0566 2985.2864 6106.5 383 3.49 20.4 1 R.SSVRDESPSVRFPNIVTNVGNQPGGITR.G YOL107W 2 2 13.7% 342 39742 9.4 U YOL107W SGDID:S000005467, Chr XV from 112101-113129, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_37.13350.13350.4 3.2806 0.1452 10.4 3582.0566 3580.118 6971.6 479 3.692 17.7 1 N.SQGGQEILVGDASDTFQLIYFFPDLIKPILRP.I * Jamie_Ashy_SPB_19.06741.06741.2 2.0197 0.1017 17.1 1756.4122 1758.0703 4630.1 328 3.433 32.1 1 K.FKVIKPFHDIDIDIG.N Reverse_YDL129W 2 2 13.7% 291 32916 9.5 U YDL129W SGDID:S000002287, Chr IV from 231024-231899, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_67.24153.24153.2 1.5511 0.0868 10.5 2665.392 2668.0654 8791.5 5 4.147 25.0 1 K.LFEVEKKLLNTEVAYRGESTARL.E * Jamie_Ashy_SPB_98.34974.34974.2 1.5616 0.0853 10.6 1864.9722 1866.0409 7330.1 120 3.564 31.2 1 A.PSFVSTNESKAERTGVR.E Reverse_YDR195W 4 4 13.5% 533 59819 9.6 U REF2 SGDID:S000002603, Chr IV from 848594-850195, Verified ORF, "RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes" * Jamie_Ashy_SPB_5.01620.01620.2 1.7432 0.0304 10.3 1221.2322 1219.519 4664.1 2 3.237 61.1 1 Y.KNRKVIPVHK.Y * Jamie_Ashy_SPB_78.28022.28022.2 1.8387 0.0291 10.4 1375.4521 1376.6824 4305.0 32 3.116 41.7 1 R.LLLSNKGFAKAIT.P * Jamie_Ashy_SPB_52.18458.18458.4 3.2723 0.0978 16.3 3983.2166 3986.4866 7204.1 120 3.736 16.2 1 I.INSAVTSAEPNVKFDVDISRKTGLKTMDADDKNLYK.S * Jamie_Ashy_SPB_79.28484.28484.2 1.7244 0.0582 13.2 1533.9521 1532.7899 4983.8 405 3.367 37.5 1 D.IRALQISSLKCDK.N YPR138C 2 2 13.5% 489 53690 6.5 U MEP3 SGDID:S000006342, Chr XVI from 812449-810980, reverse complement, Verified ORF, "Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease" * Jamie_Ashy_SPB_71.25429.25429.4 2.8205 0.1076 15.2 5020.9766 5023.863 7828.9 1 3.934 16.7 1 R.GKLFPHMVFLFVFATLVYCPITYWIWAPGGWAYQWGVLDWAGG.G * Jamie_Ashy_SPB_74.26473.26473.2 1.8536 0.0363 10.8 2379.152 2380.444 8417.2 1 4.02 29.5 1 E.DSQNSSLSSATVSGQNEKSNNPK.L YFR014C 2 2 13.5% 446 50296 6.1 U CMK1 SGDID:S000001910, Chr VI from 173869-172529, reverse complement, Verified ORF, "Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to Cmk2p and mammalian Cam Kinase II" * Jamie_Ashy_SPB_60.21375.21375.4 3.7513 0.0308 14.1 4344.4966 4346.859 7837.2 18 3.848 16.2 1 G.SLGYVAPEVLTQDGHGKPCDIWSIGVITYTLLCGYSAFR.A * Jamie_Ashy_SPB_28.09800.09800.2 1.5477 0.0056 63.5 2291.4722 2291.6934 6379.3 37 2.777 30.0 1 K.LKSELTSKAFAQLVNTVLAEK.E Reverse_YHR107C 2 2 13.5% 407 46668 8.0 U CDC12 SGDID:S000001149, Chr VIII from 328039-326816, reverse complement, Verified ORF, "Component of the septin ring of the mother-bud neck that is required for cytokinesis; septins recruit proteins to the neck and can act as a barrier to diffusion at the membrane, and they comprise the 10nm filaments seen with EM" * Jamie_Ashy_SPB_30.10645.10645.4 2.0578 0.0964 52.8 5591.8965 5590.342 7798.3 237 3.239 11.7 1 R.RYTEYHMEQTTSILDLLYTRLLLARLKRFDCHSDNEIEVLGWPYK.R * Jamie_Ashy_SPB_1.00329.00329.2 1.4143 0.0731 70.0 1334.9321 1334.4777 5135.9 264 3.697 44.4 1 K.KTRYPQQEQR.M YPR040W 2 2 13.5% 356 41020 7.9 U TIP41 SGDID:S000006244, Chr XVI from 647302-648372, Verified ORF, "Protein that interacts physically and genetically with Tap42p, which regulates protein phosphatase 2A; component of the TOR (target of rapamycin) signaling pathway" * Jamie_Ashy_SPB_56.19933.19933.3 2.2672 0.013 38.8 3357.3245 3360.8252 7078.1 139 2.799 19.0 1 K.RNTPPLRSSGINTIQINAAREMHAQTVRAR.R * Jamie_Ashy_SPB_37.13335.13335.2 1.5145 0.0859 74.7 2070.8523 2072.3928 7424.0 404 3.095 26.5 1 K.AALRDSNWVAQNTPMIKR.Q YPL158C 4 4 13.3% 758 84846 6.7 U YPL158C SGDID:S000006079, Chr XVI from 254309-252033, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_31.11202.11202.2 2.1605 0.0485 14.9 1532.2122 1532.9565 5754.6 61 3.241 45.8 1 Y.FTSLGIKIKKKLR.K * Jamie_Ashy_SPB_7.02311.02311.2 1.6319 0.1139 15.6 1351.5521 1351.5913 6055.1 47 3.643 50.0 1 R.RLFSYNVQKVP.S * Jamie_Ashy_SPB_70.24939.24939.2 1.5592 0.1732 10.1 3119.7522 3118.6035 7589.5 3 3.967 24.1 1 L.VPVLAPQKKTKPLTVDTKFKANHPQSED.S * Jamie_Ashy_SPB_73.26066.26066.4 2.3269 0.0365 43.8 5271.177 5271.852 8225.8 432 3.109 12.5 1 R.TNSSIRRAASILTASATMTPANNKNSFISVPDNVSHAVTRNSSMYSRSR.L Reverse_YGL252C 3 3 13.3% 588 65572 8.0 U RTG2 SGDID:S000003221, Chr VII from 27484-25718, reverse complement, Verified ORF, "Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p" * Jamie_Ashy_SPB_105.37500.37500.2 1.7733 0.0662 80.2 1833.9122 1831.0842 4818.0 18 3.484 42.9 1 K.TWWVIRLAEAKDGER.L * Jamie_Ashy_SPB_102.36710.36710.2 1.3506 0.1212 10.2 3090.9321 3093.5312 10724.7 104 3.725 19.6 1 L.ARARHSLGHCGAIIGRTAVHLAATPQLEK.P * Jamie_Ashy_SPB_53.18876.18876.3 2.6481 0.16 14.4 3601.8245 3597.9202 5640.6 184 3.519 15.9 1 K.WGTSGYVANVFEDANIADRTAETAIVRVSTEPVG.F YGL028C 2 2 13.3% 542 56447 4.5 U SCW11 SGDID:S000002996, Chr VII from 442914-441286, reverse complement, Verified ORF, "Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p" * Jamie_Ashy_SPB_86.30835.30835.4 3.2334 0.1488 11.3 3921.2166 3923.7317 5985.7 11 3.429 17.9 1 S.TESTNTPTSSSTSSSTSSSTSSSTSSSTSSSTSSSTSSSTSS.S * Jamie_Ashy_SPB_18.06417.06417.4 3.8583 0.295 100.0 2940.5366 2938.0417 4676.9 182 4.816 20.1 1 T.AATTSEGSSSSSAAITSSPKAIAYSPYNDD.G YBL029W 2 2 13.3% 376 42604 6.4 U YBL029W SGDID:S000000125, Chr II from 166137-167267, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_81.28893.28893.3 1.0835 0.0442 32.7 4826.7544 4830.2427 3556.2 308 2.981 5.8 1 K.PCHDSYTHSSPSSSESKKISDSRLSAEGLAKVLNLESPEEALKR.E * Jamie_Ashy_SPB_2.00652.00652.2 2.2105 0.0663 46.6 884.7922 886.04803 3992.6 1 3.633 90.0 1 S.RLRRER.R Reverse_YIL093C 2 2 13.3% 264 30513 6.3 U RSM25 SGDID:S000001355, Chr IX from 188781-187987, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the small subunit" * Jamie_Ashy_SPB_18.06343.06343.2 1.759 0.104 91.0 976.65216 976.0366 5845.7 137 3.514 56.2 1 K.WSGAPNSTR.A * Jamie_Ashy_SPB_4.01209.01209.4 2.6628 0.1896 11.1 2977.0566 2980.3447 4986.4 37 3.605 22.0 1 T.EEVVKKEWVDLVEQEKQIGHEIASVG.F YCL024W 4 4 13.2% 1037 116475 8.7 U KCC4 SGDID:S000000529, Chr III from 79161-82274, Verified ORF, "Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p" * Jamie_Ashy_SPB_77.27453.27453.4 1.8992 0.0158 33.5 6234.8564 6228.9224 8756.1 6 2.543 11.6 1 R.HSREFSSSRKRSSFLLSSNPTDSSPIPLRSSKRITHINVASANTQATPSGVPNPHKR.N * Jamie_Ashy_SPB_90.32361.32361.4 2.706 0.0227 51.2 4756.177 4756.3013 8293.8 5 3.193 15.8 1 K.KRSSKRLSYMPNTKRSSLTSKSLSNFTNLIDDDDWEYIEK.D * Jamie_Ashy_SPB_10.03387.03387.2 2.1276 0.0077 12.4 1097.9521 1098.2906 4170.3 8 4.225 55.0 1 L.LNVRGGLSPGK.S * Jamie_Ashy_SPB_35.12322.12322.3 2.3766 0.0492 49.4 3230.7244 3229.4126 7083.2 229 3.514 19.6 1 K.DGDNVNNDNILSDFPQGVGISQEYDMKDK.N YGR054W 2 2 13.2% 642 71305 8.9 U YGR054W SGDID:S000003286, Chr VII from 596697-598625, Uncharacterized ORF, "yeast homolog of mammalian eIF2A" * Jamie_Ashy_SPB_92.33002.33002.4 3.0647 0.1277 13.2 3611.1765 3611.21 8829.2 328 3.68 17.7 1 V.LSPCGRFLALSTKENVKVFTGPCLDNVLLTMK.L * Jamie_Ashy_SPB_104.37201.37201.4 2.0557 0.0559 42.3 5859.7764 5862.4023 8851.9 415 3.455 10.9 1 K.IVKLSSANADNFDFQSPFAVLSDDETSQHFTTYLISPAEHPTICTFTPEKGGK.P YLR195C 2 2 13.2% 455 52838 7.4 U NMT1 SGDID:S000004185, Chr XII from 543306-541939, reverse complement, Verified ORF, "N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction" * Jamie_Ashy_SPB_33.11725.11725.2 1.7321 0.0887 13.2 2571.652 2569.8494 6369.6 1 3.994 30.4 1 P.DGHTEEDMIAENALPAKTKTAGLR.K * Jamie_Ashy_SPB_102.36429.36429.4 2.8883 0.1041 16.0 4207.177 4210.7065 7684.7 326 3.402 15.2 1 N.MDVFNALTSQDNTLFLDDLKFGPGDGFLNFYLFNYR.A Reverse_YDL028C 3 3 13.1% 764 86828 8.9 U MPS1 SGDID:S000002186, Chr IV from 403288-400994, reverse complement, Verified ORF, "Dual-specificity kinase required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, and checkpoint protein Mad1p" * Jamie_Ashy_SPB_20.07030.07030.3 1.8624 0.0105 33.2 2457.1143 2457.946 6591.4 3 2.504 26.2 1 R.ILDEMISGSIMFPQLFTSSLVK.D * Jamie_Ashy_SPB_57.20341.20341.4 2.7878 0.1579 11.4 4087.2966 4081.4014 7383.0 124 3.458 17.2 1 R.REKFNTQHSDELPLEVTERANIPQNPSNPVDEDFI.N * Jamie_Ashy_SPB_72.25642.25642.3 1.8046 0.0334 33.3 4733.9346 4732.219 6933.4 283 2.995 11.9 1 R.LYRGFLTSHSSTSTHGSTLNGMSPPPHSRSSSRDMEELRNAIK.D YNL209W 3 3 12.9% 613 66595 5.5 U SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; homolog of SSB1" Jamie_Ashy_SPB_71.25268.25268.2 1.1597 0.2151 15.3 3382.7522 3380.8284 6171.8 262 4.2 12.5 1 D.FDTNLLEHFKAEFKKKTGLDISDDARALR.R * Jamie_Ashy_SPB_116.41562.41562.2 1.104 0.0798 61.6 2811.3123 2809.3801 6114.8 40 3.889 15.4 1 K.DLLLLDVAPLSLGVGMQGDIFGIVVPR.N Jamie_Ashy_SPB_53.19102.19102.2 2.4188 0.1982 100.0 2412.2722 2412.6562 7292.8 1 5.062 34.1 1 K.IEAALSDALAALQIEDPSADELR.K Reverse_YNL009W 2 2 12.9% 420 47856 9.2 U IDP3 SGDID:S000004954, Chr XIV from 614822-616084, Verified ORF, "Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids" * Jamie_Ashy_SPB_57.20208.20208.4 2.9102 0.0931 12.6 4653.4565 4653.2817 8922.2 229 3.112 13.8 1 I.SDNTNFMAMAIGGSKPFEYVKLDINEKGDDSSFQLRLKGAKK.I * Jamie_Ashy_SPB_7.02539.02539.2 1.9979 0.0156 12.7 1469.4922 1471.9103 5509.6 9 2.771 59.1 1 D.LYPLILKEKILK.W contaminant_KERATIN21 4 4 12.9% 357 39219 5.2 U no description Jamie_Ashy_SPB_12.04278.04278.3 2.709 0.1939 98.8 1309.1943 1309.4215 4923.1 113 4.503 47.2 1 K.NKYEDEINKR.T Jamie_Ashy_SPB_5.01832.01832.2 2.1983 0.1982 81.2 1194.6921 1195.3176 4523.2 20 4.61 75.0 1 N.KYEDEINKR.T * Jamie_Ashy_SPB_41.14756.14756.2 1.612 0.0768 12.0 1224.7922 1227.3658 4048.7 13 3.174 61.1 1 Q.RLRSESDHVK.K * Jamie_Ashy_SPB_18.06345.06345.3 2.2449 0.1035 66.9 2422.1943 2419.608 5694.7 9 3.474 24.0 1 R.AIGGGLSSVGGGSSTIKYTTTSSSSR.K contaminant_FCGA_HUMAN 2 2 12.9% 317 34990 6.8 U owl|P12318| LOW AFFINITY IMMUNOGLOBULIN GAMMA FC RECEPTOR II-A PRECURSOR (FC-GAMMA... * Jamie_Ashy_SPB_36.12972.12972.2 1.8641 0.095 16.5 1882.6522 1880.2352 6635.0 129 3.777 30.6 1 P.LTVLLLLASADSQAAAPPK.A * Jamie_Ashy_SPB_56.20122.20122.2 1.9312 0.1856 50.4 2275.2922 2272.7893 6910.9 55 4.077 23.8 1 A.VVIATAVAAIVAAVVALIYCRK.K Reverse_YLR190W 2 2 12.8% 491 54817 7.6 U MMR1 SGDID:S000004180, Chr XII from 535216-536691, Verified ORF, "Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p" * Jamie_Ashy_SPB_60.21422.21422.4 3.5872 0.1224 10.9 3073.4966 3072.3123 5647.6 58 3.796 21.4 1 L.NLQFGRKSEGSQVSATTSIASTPEEEFAV.N * Jamie_Ashy_SPB_83.29898.29898.3 2.0389 0.0125 34.5 3617.4844 3619.1123 8853.6 5 2.782 19.7 1 K.SLSTPYLLSNGIPGTDLKLKTPSNLLNQFSGANR.Q YLL029W 2 2 12.7% 749 84924 6.9 U YLL029W SGDID:S000003952, Chr XII from 81460-83709, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_89.31875.31875.4 1.1756 0.0177 32.9 6645.5366 6647.314 2771.5 349 3.046 5.3 1 R.QGRRASLFLRQLENSRRSSSMLLNELKGAGGGSSAGNGSVYSCDSLCAVNREVNTTDRLLK.L * Jamie_Ashy_SPB_95.33950.33950.4 3.7206 0.0942 11.2 3690.4966 3694.3708 7248.8 121 3.498 17.2 1 N.ANGAIAKKMTAQNFAIIHSPIDVLKSIKNDIEIK.N Reverse_YDL128W 2 2 12.7% 411 44646 5.7 U VCX1 SGDID:S000002286, Chr IV from 232652-233887, Verified ORF, "Vacuolar H+/Ca2+ exchanger involved in control of cytosolic Ca2+ concentration; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter" * Jamie_Ashy_SPB_18.06321.06321.3 2.8447 0.1192 17.8 2896.1343 2898.376 7769.2 79 3.97 22.1 1 V.NDITGVLFDACFSIIVTTGLLIVLSSK.V * Jamie_Ashy_SPB_61.21678.21678.3 2.8274 0.1188 18.0 2582.5745 2583.0735 8053.1 19 3.861 25.0 1 K.GHPLTARFAAPILLSACAIALLSSM.T Reverse_YKR067W 4 4 12.5% 743 83645 9.5 U GPT2 SGDID:S000001775, Chr XI from 567560-569791, Verified ORF, "Glycerol-3-phosphate acyltransferase located in both lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone, which are intermediate steps in lipid biosynthesis" * Jamie_Ashy_SPB_71.25348.25348.4 2.945 0.1614 10.4 2992.6565 2994.2664 5157.5 55 3.672 21.4 1 L.ANSALSGISHISSSRSRSQVSMRSISSEA.D * Jamie_Ashy_SPB_4.01167.01167.2 1.6679 0.1231 16.0 1546.4521 1545.6957 5059.6 33 3.731 42.9 1 N.SALSGISHISSSRSR.S * Jamie_Ashy_SPB_53.19054.19054.4 2.8712 0.1717 11.8 5269.3364 5271.1636 9101.0 6 3.981 14.4 1 G.PYVISVFLPRLSKFLDVGIEGTKLSSYTTFVLLAYFYVFQIFANNS.P * Jamie_Ashy_SPB_103.36820.36820.3 2.1853 0.0172 40.4 2284.4043 2287.6702 3940.8 325 2.897 26.5 1 K.RNYDYVLKKLHIIRPDDK.F Reverse_YBR214W 2 2 12.5% 527 57187 8.9 U SDS24 SGDID:S000000418, Chr II from 651410-652993, Verified ORF, "One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis" * Jamie_Ashy_SPB_86.30686.30686.3 2.3747 0.1778 16.6 3740.2144 3737.2603 9324.0 82 4.235 18.0 1 R.CTKHLLPYQSTRTVHKVDTVSINGLLNGQKDIV.A * Jamie_Ashy_SPB_64.22752.22752.4 2.9387 0.1649 11.4 3468.9766 3473.0405 7082.5 53 4.471 18.8 1 Q.SLIGKVKTMEENTIAVRHVGSGLIGMVTSLSEN.E YDR423C 3 3 12.5% 409 45752 7.1 U CAD1 SGDID:S000002831, Chr IV from 1319265-1318036, reverse complement, Verified ORF, "AP-1-like bZIP transcriptional activator involved in multiple stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins, binds consensus sequence TTACTAA; 5' UTR contains uORFs" * Jamie_Ashy_SPB_74.26486.26486.2 1.7539 0.0113 67.5 1970.3722 1970.3872 8319.5 43 3.12 28.1 1 K.KQMLLNKDGTPKRKVGR.P * Jamie_Ashy_SPB_30.10515.10515.4 2.8341 0.0506 69.3 2836.5764 2834.3098 4442.5 418 2.705 21.2 1 R.AFRDRKEAKMKSLQERVELLEQK.D * Jamie_Ashy_SPB_78.27805.27805.2 1.6756 0.1293 16.2 1329.7322 1329.5023 4772.0 2 3.509 50.0 1 K.EIKCDLITSHL.L YHR198C 2 2 12.5% 321 36502 9.1 U YHR198C SGDID:S000001241, Chr VIII from 497280-496315, reverse complement, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * Jamie_Ashy_SPB_50.17763.17763.2 1.674 0.1823 29.8 2748.112 2745.1943 6905.6 63 4.072 25.0 1 Y.LHKYFLDVDDSKTPKENLARLLK.R * Jamie_Ashy_SPB_100.35761.35761.2 1.5457 0.0085 63.5 1854.4722 1853.0854 6751.0 34 3.301 37.5 1 K.TISKHPDVANNKDTLAK.G YOR362C 2 2 12.5% 288 31536 5.2 U PRE10 SGDID:S000005889, Chr XV from 1018742-1017876, reverse complement, Verified ORF, "20S proteasome alpha-type subunit" * Jamie_Ashy_SPB_87.31231.31231.2 1.6181 3.0E-4 63.6 2253.8123 2255.548 9071.1 33 2.816 28.9 1 K.IQVVDRHIGCVYSGLIPDGR.H * Jamie_Ashy_SPB_120.43197.43197.3 1.2827 0.0765 33.6 1756.6144 1758.9749 6876.0 347 3.219 23.3 1 K.LVDHHPEGLSAREAVK.Q Reverse_contaminant_GR78_PLAFA 2 2 12.5% 279 30657 4.9 U owl|P12794| 78 KD GLUCOSE REGULATED PROTEIN HOMOLOG (GRP 78) (IMMUNOGLOBULIN... * Jamie_Ashy_SPB_41.14768.14768.3 2.9234 0.1088 17.7 2708.8743 2709.093 6960.6 7 3.616 25.0 1 V.TAKMSQIYNDLNNKAEVKERLNK.D Jamie_Ashy_SPB_4.01209.01209.2 2.3271 0.0335 16.9 1489.0322 1489.7734 4986.4 22 3.455 59.1 1 D.NIMKEIQEKSLR.G YMR208W 2 2 12.4% 443 48460 5.6 U ERG12 SGDID:S000004821, Chr XIII from 684466-685797, Verified ORF, "Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate" * Jamie_Ashy_SPB_92.33096.33096.3 1.6325 0.0361 33.5 4949.3345 4947.915 7616.8 16 3.307 12.8 1 R.STKDLVARVRVLVTEKFPEVMKPILDAMGECALQGLEIMTKLSK.C * Jamie_Ashy_SPB_39.13914.13914.2 1.7652 0.1268 21.1 1418.7522 1419.661 7427.9 21 3.455 60.0 1 N.ELYEQLLELIR.I YLR212C 2 2 12.3% 473 52628 4.7 U TUB4 SGDID:S000004202, Chr XII from 566283-564862, reverse complement, Verified ORF, "Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body" * Jamie_Ashy_SPB_76.27196.27196.4 4.2975 0.3571 100.0 6612.9766 6610.9644 7531.2 1 6.063 13.7 1 F.QSMQNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHK.L * Jamie_Ashy_SPB_76.27196.27196.3 5.5796 0.5239 100.0 4959.9844 4960.224 7531.2 1 9.687 23.3 1 R.EVVQSLMEDYVAAEQDSYLDDVLVDDENMVGELEEDLDADGDHK.L Reverse_YMR228W 2 2 12.3% 341 39727 6.6 U MTF1 SGDID:S000004841, Chr XIII from 724625-725650, Verified ORF, "Mitochondrial RNA polymerase sigma-like specificity factor required for promoter recognition, interacts with mitochondrial core polymerase Rpo41p, imported into mitochondria via a novel process requiring most of the Mtf1p sequence" * Jamie_Ashy_SPB_33.11739.11739.2 1.3775 0.115 10.1 2820.7922 2822.1711 5265.8 1 3.467 25.0 1 D.TIRSNFYQQGGHGLSDMVTNLPTRK.L * Jamie_Ashy_SPB_78.28032.28032.2 1.8437 0.133 22.4 1995.3922 1997.388 6757.4 7 3.435 37.5 1 R.KLIQLPSGEFKANLFKY.L YPL068C 2 2 12.3% 293 33098 10.1 U YPL068C SGDID:S000005989, Chr XVI from 425093-424212, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_21.07287.07287.2 2.1709 0.0671 16.5 1688.7322 1687.8009 6229.8 10 3.234 43.8 1 R.NQTSDPPSTTTAAVPSI.I * Jamie_Ashy_SPB_44.15826.15826.2 1.5697 0.1933 26.0 2602.0723 2603.2314 5887.7 15 4.053 26.1 1 A.AVPSIIVPKKRKVVAQNMVSPAIR.A Reverse_YDR106W 2 2 12.3% 284 33018 7.8 U ARP10 SGDID:S000002513, Chr IV from 666999-667853, Verified ORF, "Actin-related protein" * Jamie_Ashy_SPB_29.10320.10320.2 1.7418 0.0571 77.2 1967.2522 1967.38 6628.5 261 3.347 33.3 1 K.PFQIKSTDMKLKFLNR.I * Jamie_Ashy_SPB_90.32346.32346.2 1.6122 0.0887 12.1 2231.2722 2229.442 6783.8 177 3.273 25.0 1 R.TNCSLCHVIARPVFSVHAC.D Reverse_YDL048C 2 2 12.2% 490 54367 9.0 U STP4 SGDID:S000002206, Chr IV from 368211-366739, reverse complement, Verified ORF, "Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p" Jamie_Ashy_SPB_50.17892.17892.2 1.4674 0.0123 62.0 2273.0522 2274.553 9793.4 49 2.712 32.4 1 K.PPYEFHLAKLHNKFTDCR.S * Jamie_Ashy_SPB_75.26882.26882.3 1.8867 0.2488 12.5 4608.1743 4612.634 5711.4 8 3.99 13.4 1 S.AALKDNSDDSDNNASTRATDDQDDAGSNNDSERAYIQMFEDK.W YBR025C 2 2 12.2% 394 44174 7.4 U YBR025C SGDID:S000000229, Chr II from 291865-290681, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_73.26031.26031.2 1.6206 0.1101 15.0 2776.6921 2774.1033 10023.2 160 4.026 21.7 1 D.AEIIHVEGDVDPVRDLEIINQELR.L * Jamie_Ashy_SPB_55.19818.19818.3 3.1569 0.09 17.6 2591.0942 2588.967 7073.2 71 3.472 26.1 1 I.EFAQKALEGAEKIAKRGGQSLEVK.Q Reverse_YNR074C 2 2 12.2% 378 41335 7.4 U AIF1 SGDID:S000005357, Chr XIV from 778738-777602, reverse complement, Verified ORF, "Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase" * Jamie_Ashy_SPB_98.35175.35175.2 1.5617 0.045 68.3 2007.1122 2008.2847 6609.9 474 3.041 27.8 1 R.FNTVDGIAFVNGAEVAKVR.F * Jamie_Ashy_SPB_37.13177.13177.3 2.9785 0.1394 12.7 2952.1443 2952.3025 6715.5 65 3.912 22.1 1 K.FLLEGALECNVFGGGLFLIHDADSIRS.A YPR190C 3 3 12.1% 654 74017 6.7 U RPC82 SGDID:S000006394, Chr XVI from 919037-917073, reverse complement, Verified ORF, "RNA polymerase III subunit C82" * Jamie_Ashy_SPB_41.14481.14481.3 2.7384 0.2104 26.7 4413.054 4411.1284 6703.0 49 4.187 14.9 1 K.HYKNIPRNSPLSDLKKRSQAKMNAKTDFAKIINKPNEL.S * Jamie_Ashy_SPB_46.16495.16495.2 1.5237 0.089 10.7 1797.3722 1799.1643 6707.1 473 2.944 30.0 1 L.SQILTVDPKTSLRIVK.P * Jamie_Ashy_SPB_58.20595.20595.2 1.5896 0.1256 15.8 2945.9922 2946.377 5409.4 202 3.783 18.8 1 M.KEKDIRSTLASLIRYNSVEIQEVPR.T YAL047C 5 5 12.1% 622 72105 5.1 U SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization" * Jamie_Ashy_SPB_58.20845.20845.2 1.7807 0.0777 12.3 1972.9521 1975.1525 7941.7 477 3.796 26.5 1 D.DSEFTNSMDSGMSIPSLR.D * Jamie_Ashy_SPB_36.12679.12679.2 2.835 0.0674 97.8 1458.7122 1457.6671 9872.4 3 3.777 70.8 1 R.NSIIEGLNLEINK.L * Jamie_Ashy_SPB_17.05976.05976.2 2.856 0.3169 100.0 2452.4922 2452.6426 9478.8 1 6.787 42.1 1 F.ILTHFNEQDENAHLLDKEER.E * Jamie_Ashy_SPB_11.03721.03721.2 2.2271 0.1429 51.5 1247.1522 1247.3073 4320.3 9 4.383 63.6 1 A.QSSPAGSQLESR.D * Jamie_Ashy_SPB_34.12228.12228.2 1.6103 0.0729 77.7 1454.8522 1455.694 5356.1 6 2.944 54.5 1 K.LILSQYESNFIK.F YLL024C 2 2 12.1% 639 69470 5.1 U SSA2 SGDID:S000003947, Chr XII from 97484-95565, reverse complement, Verified ORF, "ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall" Jamie_Ashy_SPB_71.25278.25278.2 1.9005 0.1357 24.4 1711.0122 1711.878 6497.3 137 3.822 36.7 1 K.AVGIDLGTTYSCVAHF.S * Jamie_Ashy_SPB_78.27907.27907.4 2.5778 0.1844 11.1 6602.3364 6607.372 9911.9 13 4.041 11.7 1 K.SEVFSTYADNQPGVLIQVFEGERAKTKDNNLLGKFELSGIPPAPRGVPQIEVTFDVDSNGI.L YOR177C 2 2 12.1% 464 54358 7.8 U MPC54 SGDID:S000005703, Chr XV from 667179-665785, reverse complement, Verified ORF, "Component of the meiotic outer plaque, a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate" * Jamie_Ashy_SPB_9.02946.02946.2 1.8306 0.0867 14.9 1216.6322 1215.3951 2837.8 15 3.81 55.0 1 I.TPNKSSLKSPR.G * Jamie_Ashy_SPB_13.04399.04399.4 2.9272 0.0879 10.8 5304.8164 5304.965 3956.0 457 3.581 13.3 1 I.DKSMKKIEHLERSLQTQFTAQNSFSTAMIQEEGPKDAHLKDRYHK.V Reverse_YNR041C 2 2 12.1% 372 41001 10.0 U COQ2 SGDID:S000005324, Chr XIV from 701661-700543, reverse complement, Verified ORF, "Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis" * Jamie_Ashy_SPB_54.19364.19364.2 1.863 0.0733 15.1 2216.2322 2215.6592 6215.2 107 3.473 26.2 1 G.WLLGSNLGAVALLAIQSASMAK.M * Jamie_Ashy_SPB_37.13045.13045.4 2.5394 0.0208 47.8 2675.1365 2677.1316 5240.2 3 2.408 25.0 1 R.RPSVRGSAIPREVSRIVRQDLKR.D Reverse_YJL203W 2 2 12.1% 280 33052 7.9 U PRP21 SGDID:S000003739, Chr X from 53341-54183, Verified ORF, "Subunit of the SF3a splicing factor complex, required for spliceosome assembly" * Jamie_Ashy_SPB_89.32022.32022.2 1.5823 0.022 65.3 1424.9122 1427.7366 5051.5 68 2.997 50.0 1 K.KLRTEGVARIKR.K * Jamie_Ashy_SPB_66.23572.23572.2 1.3281 0.1467 12.1 2723.2122 2723.1038 8243.7 7 3.785 26.2 1 D.KKIFSFREDELLKNEFEVGHQK.I Reverse_YNL144C 3 3 12.0% 740 84202 6.5 U YNL144C SGDID:S000005088, Chr XIV from 355044-352822, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_72.25860.25860.3 2.7839 0.1375 16.7 3263.0344 3259.4785 8011.3 28 3.803 21.3 1 P.FDHNLCGTVSFSKTSFITGYEVDANEYR.S * Jamie_Ashy_SPB_17.05876.05876.2 1.9473 0.0763 15.0 1746.4521 1745.8032 4406.0 271 3.092 42.9 1 E.DGNVDENGRNLKEER.N * Jamie_Ashy_SPB_73.26306.26306.4 3.0294 0.0971 17.9 4786.3364 4791.0386 7751.7 374 3.063 12.2 1 F.LGCFDTQSSEGALSGSDNPGNSDHSADSVSPPLLSLSRSRSKNTPR.E YMR227C 3 3 12.0% 590 67556 4.6 U TAF7 SGDID:S000004840, Chr XIII from 724384-722612, reverse complement, Verified ORF, "TFIID subunit (67 kDa), involved in RNA polymerase II transcription initiation" * Jamie_Ashy_SPB_33.11838.11838.3 3.3062 0.1469 17.0 3131.0645 3126.6677 6403.8 312 3.983 19.2 1 K.KKKESADGKEKKNSLKLKLNLKKNEEP.V * Jamie_Ashy_SPB_57.20456.20456.4 2.9764 0.1643 10.6 2633.5764 2633.8682 3774.8 344 3.525 26.5 1 E.EEEVYALEAPDTEDLVVKHFEGI.E * Jamie_Ashy_SPB_33.11569.11569.3 2.2331 0.048 46.9 2401.9143 2401.6812 5525.9 275 2.936 25.0 1 R.VSSWENFKEEPGEPLSRPALK.K YBR103W 2 2 12.0% 535 59161 5.0 U SIF2 SGDID:S000000307, Chr II from 447703-449310, Verified ORF, "WD40 repeat-containing subunit of the Set3C histone deacetylase complex, which represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus" * Jamie_Ashy_SPB_37.13212.13212.3 2.6364 0.1584 15.0 2907.7744 2905.3237 6113.8 52 3.39 20.2 1 K.QNTLLALSIVDGVPIFAGRISQDGQKY.A * Jamie_Ashy_SPB_47.16778.16778.4 3.4101 0.1286 11.2 4705.2563 4700.3696 8351.4 9 3.472 15.9 1 Q.DGQKYAVAFMDGQVNVYDLKKLNSKSRSLYGNRDGILNPLPI.P Reverse_YNR051C 2 2 12.0% 515 57674 9.1 U BRE5 SGDID:S000005334, Chr XIV from 718329-716782, reverse complement, Verified ORF, "Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A" * Jamie_Ashy_SPB_77.27483.27483.4 2.9612 0.1016 17.3 4180.8564 4181.5747 6651.1 427 3.213 15.8 1 K.SAWTMKPPNAVPEPSSSPTSANNGSEKADSVQPEIPKIET.E * Jamie_Ashy_SPB_85.30512.30512.2 1.5942 0.1593 17.0 2403.0322 2405.6648 8214.2 330 3.987 23.8 1 I.PKEQTSSETPATTDKVTEKKAK.P Reverse_YKL048C 3 3 11.9% 640 72150 8.1 U ELM1 SGDID:S000001531, Chr XI from 348780-346858, reverse complement, Verified ORF, "Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases; forms part of the bud neck ring" * Jamie_Ashy_SPB_39.13806.13806.2 1.7888 0.1221 19.2 1509.7922 1512.6691 6958.1 22 3.659 45.8 1 D.PSSRLVTTSFCEK.D * Jamie_Ashy_SPB_30.10531.10531.4 3.0179 0.0911 15.7 5199.4966 5200.0156 4172.9 30 2.983 13.5 1 K.STASVLVKDKGKGSTKKKGKKTLLRGFFRRVPGENRVPNVSSSSIQSH.N * Jamie_Ashy_SPB_92.33156.33156.2 1.6686 0.1042 15.0 1710.6921 1712.0458 5405.2 37 3.895 39.3 1 L.LRKIVLDNLTNGNIK.S YNL004W 2 2 11.9% 429 49142 6.6 U HRB1 SGDID:S000004949, Chr XIV from 623334-624623, Verified ORF, "Poly(A+) RNA-binding protein, involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Gbp2p and Npl3p" * Jamie_Ashy_SPB_96.34357.34357.2 1.6726 0.0733 13.3 2205.0122 2206.3052 9508.0 67 3.655 31.2 1 R.DNPRSRDRYDDRRRGRD.V * Jamie_Ashy_SPB_30.10525.10525.4 3.0417 0.1291 12.5 3828.5764 3834.3179 6888.4 3 3.714 19.7 1 S.IFVGNLTYDSTPEDLTEFFSQIGKVVRADIITSR.G Reverse_YNR050C 2 2 11.9% 446 48918 5.3 U LYS9 SGDID:S000005333, Chr XIV from 715390-714050, reverse complement, Verified ORF, "Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, which is the seventh step in lysine biosynthesis pathway" * Jamie_Ashy_SPB_11.03871.03871.3 2.1985 0.0304 43.3 2026.5543 2027.4117 7066.6 60 3.108 32.8 1 -.AVTKEKLYIGYKDKLEK.M * Jamie_Ashy_SPB_78.28105.28105.3 1.7435 0.0208 33.4 4254.7144 4254.809 6820.9 172 2.665 15.0 1 R.NPYCVFAYGPYIFYPKATAMLDESSVTEIKGDKWYK.A Reverse_YGL090W 2 2 11.9% 421 48259 5.0 U LIF1 SGDID:S000003058, Chr VII from 343322-344587, Verified ORF, "Protein involved in DNA double-strand break repair; physically interacts with DNA ligase 4 (Lig4p); homologous to mammalian XRCC4 protein" * Jamie_Ashy_SPB_37.13288.13288.4 3.3612 0.071 18.0 3394.8564 3394.4236 6778.3 36 3.354 20.1 1 -.TEIDTESETQLCPERDNGSQESHNSSKKEG.A * Jamie_Ashy_SPB_63.22375.22375.3 2.5276 0.1189 80.7 2276.8145 2280.7307 5668.0 405 3.225 27.6 1 K.TFVVKEDLSAIYVKHGTLMK.L Reverse_YLR107W 2 2 11.9% 404 45880 8.8 U REX3 SGDID:S000004097, Chr XII from 364117-365331, Verified ORF, "RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases" * Jamie_Ashy_SPB_41.14799.14799.2 1.5986 0.0753 10.7 2264.2722 2265.7507 9608.1 19 3.315 30.6 1 I.KRSLVEFALNKLSVKFKTR.S * Jamie_Ashy_SPB_91.32599.32599.4 2.6388 0.0307 51.6 3372.6165 3371.9944 5563.5 350 2.932 19.0 1 R.TALRELDINTGKTHAYAKQLQKFLKQLIK.Y YOR184W 2 2 11.9% 395 43416 6.5 U SER1 SGDID:S000005710, Chr XV from 679357-680544, Verified ORF, "3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p" * Jamie_Ashy_SPB_5.01707.01707.3 2.1447 0.0245 42.2 1716.5944 1716.8455 6110.7 75 3.023 36.7 1 K.KGGVEAQQAENEEKAK.I * Jamie_Ashy_SPB_39.14000.14000.4 3.1866 0.0725 14.3 3623.2566 3621.1084 8899.8 160 3.236 18.9 1 K.ILYEALDANSDFYNVPVDPKCRSKMNVVFTL.K Reverse_YCL028W 2 2 11.9% 405 42580 6.6 U RNQ1 SGDID:S000000533, Chr III from 70150-71367, Verified ORF, "[PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate" * Jamie_Ashy_SPB_27.09602.09602.4 3.0529 0.1872 10.3 3961.3765 3966.1174 6101.2 400 3.496 16.2 1 N.NQGQQNYQQQNSQTQGGGLYSQAMSALSSFAGGSQGQQ.Q * Jamie_Ashy_SPB_16.05794.05794.2 1.4555 0.0535 68.8 980.8522 979.93585 4590.2 2 3.222 72.2 1 R.DQSADSGGSR.N Reverse_YLR260W 3 3 11.8% 687 77566 6.9 U LCB5 SGDID:S000004250, Chr XII from 665846-667909, Verified ORF, "Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules" * Jamie_Ashy_SPB_58.20577.20577.2 2.3566 0.2633 86.8 2054.152 2054.3716 6936.0 3 4.593 38.2 1 P.MKGTYFIGLNNSINPDLR.E * Jamie_Ashy_SPB_53.18966.18966.2 1.7438 0.1206 15.2 2379.9521 2377.5266 10074.6 21 3.923 25.0 1 K.FDEEKIQDASSDALDRSILHS.D * Jamie_Ashy_SPB_61.21662.21662.3 1.6304 0.0312 33.0 4820.9043 4824.1606 10000.0 365 3.229 12.2 1 K.KQSSELEDLDCKESINSMSSSLPSSPSSNADYYHLPEKEIYR.A Reverse_YER087W 2 2 11.8% 576 65880 7.4 U YER087W SGDID:S000000889, Chr V from 330572-332302, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_48.17104.17104.4 2.619 0.0474 57.3 3079.5366 3084.6594 6977.9 167 3.017 20.8 1 K.LYKKLPFDPFNSKSNLRCDMVRLIK.S * Jamie_Ashy_SPB_70.25168.25168.4 2.8695 0.1392 14.2 4752.177 4754.248 8224.3 25 3.696 14.7 1 K.FLESNNWRDTAQWLAKSSIASLSVEIAGGDSDMRNRIANSVKN.L Reverse_YBR056W 2 2 11.8% 501 57822 6.5 U YBR056W SGDID:S000000260, Chr II from 347877-349382, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_79.28275.28275.2 1.8807 0.0488 80.0 2124.172 2124.4448 7206.4 1 3.569 41.7 1 R.GILSNDFKRFAIIDDVATK.I * Jamie_Ashy_SPB_88.31558.31558.4 2.7707 0.1253 17.2 4728.6963 4728.411 6238.4 146 4.29 15.4 1 N.YRYKYITSRDLDKAPPLKPLTTSLNQGGKESVKFHEFKNR.L Reverse_YER127W 2 2 11.8% 357 40794 6.9 U LCP5 SGDID:S000000929, Chr V from 414477-415550, Verified ORF, "Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus" * Jamie_Ashy_SPB_55.19614.19614.3 2.2343 0.0147 38.4 2705.7244 2705.012 5684.9 1 3.234 26.1 1 R.RQARDWASRTKKAGRRSTSDELK.R * Jamie_Ashy_SPB_102.36698.36698.2 1.4017 0.0409 63.8 2097.392 2099.372 5602.3 38 2.862 30.6 1 R.KSSFIGFDEGGIVNMRANR.E Reverse_YPL131W 2 2 11.8% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_Ashy_SPB_87.31352.31352.4 2.3479 0.0812 61.1 3139.6165 3141.5576 4810.5 31 3.109 23.7 1 K.AAVRAAREEKSLKTQRYKKSEAAYQEK.T * Jamie_Ashy_SPB_42.15166.15166.2 1.3685 0.0792 67.4 3018.7522 3019.471 7405.5 36 3.677 20.8 1 K.SLKTQRYKKSEAAYQEKTFKKETPK.F contaminant_NRL_1INDL 2 3 11.8% 211 22765 6.2 U owl|| Cha255 immunoglobulin fab' fragment (igg1-lambda) complex With... * Jamie_Ashy_SPB_14.04988.04988.2 1.7643 0.0288 79.6 855.83215 856.05316 3496.9 184 3.587 64.3 1 K.LTVLGQPK.S * Jamie_Ashy_SPB_11.03648.03648.2 1.5756 0.1086 82.6 1988.4922 1987.0588 4957.4 30 3.485 37.5 2 R.HSSYSCQVTHEGHTVEK.S YFL053W 3 3 11.7% 591 62135 5.9 U DAK2 SGDID:S000001841, Chr VI from 23423-25198, Verified ORF, "Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation" * Jamie_Ashy_SPB_96.34286.34286.3 2.722 0.1418 16.7 3082.7344 3080.6418 7159.0 187 4.207 19.2 1 K.VELVAVGDDVSVGKKKGSLVGRRGLGATVLV.H * Jamie_Ashy_SPB_97.34681.34681.3 2.4028 0.0327 47.2 2578.8245 2581.9749 5598.4 2 3.25 28.4 1 K.DWEVLAKGQVPTAPSLKTLRNEK.G * Jamie_Ashy_SPB_49.17527.17527.2 1.5521 0.0381 65.8 1479.0122 1476.5889 6012.3 17 3.199 42.9 1 K.GPRAAAQAAYDGAEK.T Reverse_YNL112W 2 2 11.7% 546 60999 8.8 U DBP2 SGDID:S000005056, Chr XIV from 413641-414913,415916-416283, Verified ORF, "Essential ATP-dependent RNA helicase of the DEAD-box protein family, involved in nonsense-mediated mRNA decay and rRNA processing" * Jamie_Ashy_SPB_87.31238.31238.4 2.8273 0.1275 16.0 4986.7363 4989.5786 7741.1 11 4.192 14.0 1 R.SLDRIQQSKPVGGYVCTNRIRSSHGFKSCETQIQVALERTPALV.L * Jamie_Ashy_SPB_119.42698.42698.2 1.3459 0.195 16.6 2098.892 2098.3882 6909.1 3 4.151 31.6 1 G.PLCYSLTKGSGTAAIGVMDR.G YGR150C 4 4 11.6% 864 101422 8.6 U YGR150C SGDID:S000003382, Chr VII from 793058-790464, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_98.35019.35019.3 2.6544 0.1192 15.4 2849.6943 2851.022 6071.7 314 3.497 20.0 1 E.RIHNTKSVNSDGHQSNSIAPISEDSR.N * Jamie_Ashy_SPB_42.14995.14995.4 3.5458 0.1495 10.6 3660.3364 3661.9592 6360.3 1 4.156 22.7 1 Q.SNSIAPISEDSRNVNVTKTSSVPNEEKSKNLSDL.I * Jamie_Ashy_SPB_93.33378.33378.2 1.4974 0.1364 17.3 2249.652 2251.4202 6664.1 37 3.716 30.6 1 R.LYEGYRNIPSSHTRDQNSI.S * Jamie_Ashy_SPB_24.08401.08401.4 2.4903 0.1032 75.2 3955.5366 3954.7302 4495.8 33 3.453 17.2 1 R.TTVPFLKMDQRIRIAKDQKFAHLMVEFFTKQGK.Y YOL148C 2 2 11.6% 604 67797 9.6 U SPT20 SGDID:S000005508, Chr XV from 47572-45758, reverse complement, Verified ORF, "Subunit of the SAGA transcriptional regulatory complex, involved in maintaining the integrity of the complex" * Jamie_Ashy_SPB_69.24708.24708.2 1.9548 0.1841 50.5 1992.0122 1994.348 5901.2 50 4.11 33.3 1 R.LLLQQRLLEQQRKQQA.L * Jamie_Ashy_SPB_48.17031.17031.4 2.879 0.1704 11.5 5098.6567 5100.4746 7457.0 85 4.247 12.6 1 T.KNAMEIASSSSNGVRGASSSTSNSASNTRNNSLANGNQVALAAAAAAAAVGSTM.G Reverse_YDR393W 3 3 11.6% 456 53571 9.0 U SHE9 SGDID:S000002801, Chr IV from 1259891-1261261, Verified ORF, "Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex" * Jamie_Ashy_SPB_35.12530.12530.3 1.7779 0.0321 33.3 3645.0244 3642.2078 6851.1 456 2.988 15.8 1 K.LTVFLAKIREWIDRFSKMETTTTEATAVEPR.D * Jamie_Ashy_SPB_15.05437.05437.2 2.167 0.0087 13.0 1590.1522 1589.7863 6527.5 70 3.011 45.8 1 L.QEEKSEIEKVKNK.L * Jamie_Ashy_SPB_11.03753.03753.2 1.6428 0.0517 13.2 1067.6721 1069.2083 7246.6 35 2.666 68.8 1 H.FPRTFSSAR.L YGL171W 3 3 11.5% 564 63653 9.1 U ROK1 SGDID:S000003139, Chr VII from 182396-184090, Verified ORF, "ATP-dependent RNA helicase of the DEAD box family; required for 18S rRNA synthesis" * Jamie_Ashy_SPB_15.05274.05274.3 2.7406 0.1392 12.5 2085.5044 2089.4458 6155.8 428 3.419 26.4 1 M.DIFRVLTRGASVKKESGPK.A * Jamie_Ashy_SPB_11.03848.03848.2 2.5266 0.0401 10.6 2194.4722 2194.5388 4375.2 18 3.163 35.3 1 E.DLISRFSFDKRLLNNLIE.N * Jamie_Ashy_SPB_7.02314.02314.3 1.1337 0.0113 32.7 3254.5144 3252.8467 6061.3 483 2.882 10.2 1 R.KEKESIKNGKAHKERKQITTVPKMDKAK.R Reverse_YMR094W 2 2 11.5% 478 56336 9.1 U CTF13 SGDID:S000004700, Chr XIII from 455824-457260, Verified ORF, "Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis" * Jamie_Ashy_SPB_52.18523.18523.2 1.6292 0.2045 29.9 1860.4521 1859.1326 7066.1 162 3.756 32.1 1 F.KSDDEKIYLSKFTRK.D * Jamie_Ashy_SPB_55.19512.19512.4 3.2394 0.0665 12.8 4209.5767 4204.325 7013.3 17 3.195 16.2 1 K.ITSSPSSNEDSNAGSVDVDKEEGVEEDQGFQVEGITAFLD.K Reverse_YNL307C 2 2 11.5% 375 43136 8.6 U MCK1 SGDID:S000005251, Chr XIV from 57573-56446, reverse complement, Verified ORF, "Protein serine/threonine/tyrosine (dual-specificity) kinase involved in control of chromosome segregation and in regulating entry into meiosis; related to mammalian glycogen synthase kinases of the GSK-3 family" * Jamie_Ashy_SPB_30.10790.10790.4 2.7908 5.0E-4 46.9 3193.9365 3197.7068 5337.8 2 2.546 23.5 1 K.SFLPGDYAPNSFFIFRKDPPGLLKAIER.L * Jamie_Ashy_SPB_23.08299.08299.2 2.0192 0.0242 12.8 1640.9321 1640.8339 4953.5 71 3.448 39.3 1 V.GTEPDVLVNSPKIDR.H YDR452W 3 3 11.4% 674 78344 6.9 U PPN1 SGDID:S000002860, Chr IV from 1362868-1364892, Verified ORF, "Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer" * Jamie_Ashy_SPB_38.13382.13382.4 2.582 0.0207 47.3 4547.8164 4549.072 8726.0 33 2.863 15.4 1 K.TGKERKLHGRFLHITDIHPDPYYVEGSSIDAVCHTGKPSK.K * Jamie_Ashy_SPB_117.41938.41938.2 1.2838 0.0129 60.6 2926.7522 2925.9653 3781.9 371 3.036 18.8 1 K.AMEQSTRVQQGEDSNEEDEETELNR.I * Jamie_Ashy_SPB_71.25575.25575.2 1.7456 0.0906 86.6 1321.4521 1321.5774 3856.6 23 3.497 45.5 1 K.EVFLMGAKPSNK.E Reverse_YBR263W 2 2 11.4% 490 53686 8.7 U SHM1 SGDID:S000000467, Chr II from 736259-737731, Verified ORF, "Mitochondrial serine hydroxymethyltransferase, involved in one-carbon metabolism" * Jamie_Ashy_SPB_64.22771.22771.3 2.317 0.1791 16.9 3465.7744 3461.9658 7776.4 25 4.054 18.5 1 K.LAVAMAGITHNHPGGQHGPFVSFNIKKELEYP.I * Jamie_Ashy_SPB_74.26461.26461.2 1.5348 0.0913 11.4 2893.7522 2893.2065 6813.7 486 3.098 19.6 1 I.SHKQRHREQQLIDFMEPDGESVPK.S YML074C 2 2 11.4% 411 46553 4.5 U FPR3 SGDID:S000004539, Chr XIII from 121324-120089, reverse complement, Verified ORF, "Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p" * Jamie_Ashy_SPB_61.21957.21957.3 2.5136 0.0197 45.1 2829.3245 2825.2964 7823.1 132 3.371 21.9 1 R.ITMAALNPEAIDEENKPSTLRIIKR.N * Jamie_Ashy_SPB_33.11643.11643.2 1.9386 0.0341 13.4 2423.8323 2425.8796 5677.3 65 3.453 26.2 1 Y.IGKLKNGKVFDKNTSGKPFAFK.L YGR185C 2 2 11.4% 394 44020 8.4 U TYS1 SGDID:S000003417, Chr VII from 867525-866341, reverse complement, Verified ORF, "Cytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNA synthetase that aminoacylates tRNA(Tyr), required for cytoplasmic protein synthesis, interacts with positions 34 and 35 of the anticodon of tRNATyr" * Jamie_Ashy_SPB_98.35198.35198.4 2.5629 0.0131 44.1 3701.0566 3695.2166 6091.5 188 2.881 16.1 1 K.KKINSAFCSPGNVEENGLLSFVQYVIAPIQELK.F * Jamie_Ashy_SPB_15.05131.05131.2 1.5909 0.0829 12.6 1325.6322 1326.5327 5187.6 239 3.507 45.5 1 K.EEKLSPPDLKIG.V Reverse_YMR001C 3 3 11.3% 705 81031 9.0 U CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate" * Jamie_Ashy_SPB_50.17703.17703.3 2.1331 0.0126 35.5 2815.7944 2817.281 6226.2 186 2.5 21.7 1 R.GFTSVRSLNAKMYKAFFDVVELHR.S * Jamie_Ashy_SPB_8.02672.02672.2 1.6983 0.0587 11.6 1102.0322 1100.3005 5269.5 60 2.797 50.0 1 I.LIKGEDSIPK.D * Jamie_Ashy_SPB_116.41580.41580.4 1.6779 0.011 33.5 5585.2163 5586.449 4970.1 411 3.532 8.5 1 K.LNYNSDFFINGLKLDRHIVRRSHMYKIAGCIQTTFFRVEPETLVKR.R YDR436W 3 3 11.3% 710 78492 9.3 U PPZ2 SGDID:S000002844, Chr IV from 1334811-1336943, Verified ORF, "Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance" * Jamie_Ashy_SPB_15.05119.05119.2 2.0073 0.016 78.6 1551.4722 1552.6035 4527.8 63 2.976 45.8 1 K.HNSKKDDHDGDRK.K * Jamie_Ashy_SPB_17.05970.05970.4 2.667 0.1923 10.1 3962.8164 3964.352 7151.3 305 3.83 16.7 1 Y.LTSHPSSSRRLSSSSRRSSMGNNNNSELPPSMIQME.P * Jamie_Ashy_SPB_23.08323.08323.4 3.1143 0.1064 16.1 3084.2966 3084.2834 6480.9 27 3.211 20.0 1 D.DSGDNVNGRGTSPIPNLNIDKPSPSASSASK.R Reverse_YNL065W 3 3 11.3% 586 65296 8.8 U AQR1 SGDID:S000005009, Chr XIV from 503725-505485, Verified ORF, "Plasma membrane transporter of the major facilitator superfamily that confers resistance to short-chain monocarboxylic acids and quinidine" * Jamie_Ashy_SPB_51.18217.18217.3 3.0177 0.0909 15.0 2944.0745 2943.3389 4157.2 333 3.772 21.9 1 N.DNQDRKTGKKAKVALTNLWSQRQQK.L * Jamie_Ashy_SPB_55.19794.19794.4 2.7177 0.1502 12.1 2567.1365 2570.995 4247.2 52 3.445 27.8 1 N.IEADQPILGNAKDQEFKKIKRK.F * Jamie_Ashy_SPB_45.15906.15906.3 2.764 0.2901 93.5 2118.9844 2118.435 6580.0 359 4.501 26.4 1 S.LLTYPAKTEATIQQTPDVK.D YDR101C 3 3 11.3% 593 65213 7.2 U ARX1 SGDID:S000002508, Chr IV from 657462-655681, reverse complement, Verified ORF, "Protein associated with the ribosomal export complex" * Jamie_Ashy_SPB_85.30424.30424.4 3.0907 0.1845 10.0 2740.4966 2741.1638 5174.0 33 4.288 23.5 1 V.ALLACALTPEKLPASLGGTSSGITGQLI.R * Jamie_Ashy_SPB_96.34519.34519.2 1.3163 0.169 15.0 2959.0522 2956.319 5296.0 211 3.804 19.2 1 S.TPFTNVSAIPSDDFVVQSGEVYLIDLK.M * Jamie_Ashy_SPB_60.21562.21562.2 1.5158 0.1015 12.1 1376.1322 1375.5922 5535.2 228 3.469 40.9 1 K.MASLEHCTKKGL.V YGR091W 2 2 11.3% 494 56303 8.1 U PRP31 SGDID:S000003323, Chr VII from 666345-667829, Verified ORF, "Splicing factor, component of the U4/U6-U5 snRNP complex" * Jamie_Ashy_SPB_39.14056.14056.4 2.8335 0.1024 16.3 1990.2965 1986.2412 3847.3 3 3.563 33.3 1 G.KNKHLSHELHTLESGVR.Q * Jamie_Ashy_SPB_83.29665.29665.3 1.7052 0.02 33.2 4395.804 4392.1514 7616.6 1 2.496 17.1 1 K.AELSKKARKLSEAPSISETKALPIPEDQPKKKRAGRKFR.K YOR091W 2 2 11.3% 345 39514 9.3 U YOR091W SGDID:S000005617, Chr XV from 493433-494470, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_51.18051.18051.3 2.511 0.1218 79.7 2873.0645 2872.3586 7873.3 6 3.381 25.0 1 K.KDNVDKTFGMKNKNRSTKVQKYIK.Q * Jamie_Ashy_SPB_8.02760.02760.3 2.5883 0.1695 16.1 1715.1244 1715.0928 4057.3 208 4.168 32.1 1 V.IAKINAEKKLSSKRK.P YKL221W 2 2 11.2% 473 52342 8.8 U MCH2 SGDID:S000001704, Chr XI from 6108-7529, Verified ORF, "Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport" * Jamie_Ashy_SPB_33.11676.11676.3 3.2006 0.0795 16.5 3184.6143 3186.3818 7457.0 49 4.108 20.4 1 R.HEDHHRDVENKLNLNGKDDINGNTSISI.E * Jamie_Ashy_SPB_37.13136.13136.2 1.8473 0.2501 68.3 2623.0923 2624.156 6358.7 50 4.442 22.9 1 K.LPGTFGSTWIFMAAFALVAPIIGLE.L YDR515W 2 2 11.2% 447 50943 9.3 U SLF1 SGDID:S000002923, Chr IV from 1473419-1474762, Verified ORF, "RNA binding protein that associates with polysomes; proposed to be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts" * Jamie_Ashy_SPB_67.23950.23950.3 3.078 0.1296 12.6 3154.9143 3150.5132 8087.6 108 3.832 19.0 1 K.LTSNTKWTPITPSVIISGSKDTNSKSGKNS.K * Jamie_Ashy_SPB_57.20343.20343.3 2.5947 0.1919 19.4 2217.1443 2221.641 6299.6 31 3.94 26.3 1 F.YRMVNLSLGGDPNLILASMR.E Reverse_YER037W 2 2 11.2% 321 37719 5.4 U PHM8 SGDID:S000000839, Chr V from 225888-226853, Verified ORF, "Protein of unknown function, expression is induced by low phosphate levels and by inactivation of Pho85p" * Jamie_Ashy_SPB_61.21756.21756.2 1.6004 0.1043 12.1 2413.0923 2414.9402 8009.2 213 3.226 27.8 1 K.KKKLNILLERLKWDPKLYD.Q * Jamie_Ashy_SPB_23.08017.08017.3 2.6211 0.1482 12.0 1990.3444 1992.2358 4877.8 302 3.396 28.1 1 K.NKILGKVSLGYEQYYSE.I Reverse_YDR184C 2 2 11.2% 294 33114 6.2 U ATC1 SGDID:S000002592, Chr IV from 831508-830624, reverse complement, Verified ORF, "Nuclear protein, possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern" * Jamie_Ashy_SPB_8.02736.02736.2 1.5117 0.0772 78.4 950.97217 953.13245 5438.2 97 2.906 64.3 1 K.HTNIIRAK.M * Jamie_Ashy_SPB_16.05750.05750.2 2.7328 0.0142 19.5 2865.5122 2866.0483 6348.2 7 3.39 29.2 1 L.LRHLTHEINADDTLDTNPNSQNTNM.- YJL093C 3 3 11.1% 691 77408 7.1 U TOK1 SGDID:S000003629, Chr X from 256728-254653, reverse complement, Verified ORF, "Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin" * Jamie_Ashy_SPB_103.36916.36916.4 1.8147 0.0251 33.5 5832.0967 5833.7812 5874.3 1 2.897 11.5 1 K.NNSVVTNPRSNDTDVLMNQVKTVFDPPGIFAVNIISLVLGFTSNIILMLHFSK.K * Jamie_Ashy_SPB_15.05194.05194.2 1.8943 0.0666 14.2 1526.9122 1526.857 4012.9 161 3.42 42.9 1 C.TVSLLTVGLGDILPK.S * Jamie_Ashy_SPB_15.05114.05114.2 1.6993 0.0504 11.9 1036.7322 1036.0868 5976.4 179 2.857 62.5 1 M.DSSKNLSER.E YMR198W 2 2 11.1% 594 69070 6.4 U CIK1 SGDID:S000004811, Chr XIII from 659744-661528, Verified ORF, "Kinesin-associated protein required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; has similarity to Vik1p" * Jamie_Ashy_SPB_29.10286.10286.4 3.6395 0.1605 95.9 4307.4565 4306.8633 9299.3 12 4.164 17.6 1 K.KKLASTSQALTQVQEEKKQVEGEANNWKKKYVNELEK.V * Jamie_Ashy_SPB_23.08295.08295.4 3.3699 0.0734 84.0 3407.6165 3406.021 6726.3 53 3.745 20.2 1 K.KTLLKQLIEQYGENYKMTLNILHLDGSIK.H YPR171W 3 3 11.1% 576 64666 9.9 U BSP1 SGDID:S000006375, Chr XVI from 883824-885554, Verified ORF, "Adapter that links synaptojanins Inp2p and Inp53p to the cortical actin cytoskeleton" * Jamie_Ashy_SPB_27.09430.09430.2 1.1635 0.0376 60.8 2802.3123 2805.0361 8232.0 1 3.359 26.0 1 R.YSNIPSSKPAGEALSPVRSHNSGEYR.R * Jamie_Ashy_SPB_94.33818.33818.2 1.7858 0.0598 12.8 2013.1122 2010.4366 4595.3 352 3.161 26.5 1 M.KNKPKPTPPSPPAKRIPR.S * Jamie_Ashy_SPB_101.36178.36178.2 1.7627 0.0784 12.3 2087.5522 2086.3098 8567.0 92 4.375 28.9 1 R.NLRASNTLPELSGVNTNIAT.S YER086W 2 2 11.1% 576 63831 8.5 U ILV1 SGDID:S000000888, Chr V from 328473-330203, Verified ORF, "Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation" * Jamie_Ashy_SPB_76.27153.27153.4 2.4185 0.1697 87.8 5925.6167 5925.9014 10136.5 3 3.994 13.3 1 K.QSKVSGLNLLRLKAHLHRQHLSPSLIKLHSELKLDELQTDNTPDYVRLVLR.S * Jamie_Ashy_SPB_91.32732.32732.2 1.5596 0.0978 12.4 1433.7922 1433.7373 4450.4 201 3.126 41.7 1 K.RVAPHIKIIGVET.Y YJL008C 2 2 11.1% 568 61662 5.7 U CCT8 SGDID:S000003545, Chr X from 421578-419872, reverse complement, Verified ORF, "Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo" * Jamie_Ashy_SPB_72.25630.25630.4 2.3186 0.0071 38.9 4267.5366 4271.745 9103.3 70 2.859 16.2 1 K.QGYNSYSNADGQIIKSIAAIRELHQMCLTSMGPCGRNK.I * Jamie_Ashy_SPB_54.19210.19210.2 1.8127 0.1145 16.1 2783.872 2784.1138 7150.9 362 3.275 20.8 1 K.TMEIGGDRVTVFKQEQGEISRTSTI.I YNL274C 2 2 11.1% 350 38831 6.3 U YNL274C SGDID:S000005218, Chr XIV from 122170-121118, reverse complement, Uncharacterized ORF, "Putative hydroxyisocaproate dehydrogenase" * Jamie_Ashy_SPB_74.26493.26493.2 1.3917 0.1441 11.2 2721.5322 2719.0325 8204.7 195 3.761 22.9 1 A.LPSSVVAVCHTGAGYDQIDVEPFKK.R * Jamie_Ashy_SPB_87.31050.31050.2 1.5907 0.163 10.1 1622.7122 1624.8339 5852.2 2 3.635 42.3 1 G.KIRSAGLDVFEYEP.K YJR139C 2 2 11.1% 359 38502 7.5 U HOM6 SGDID:S000003900, Chr X from 690439-689360, reverse complement, Verified ORF, "Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions" * Jamie_Ashy_SPB_29.10485.10485.2 2.1055 0.0617 15.9 1810.1721 1810.0544 4955.5 191 3.567 39.3 1 F.LEKLSDYDKDLTQLK.K * Jamie_Ashy_SPB_48.17073.17073.2 1.8849 0.132 21.2 2249.4722 2249.6157 7477.0 260 3.652 20.8 1 T.NPVVIQGAGAGAAVTAAGVLGDVIK.I YLR348C 2 2 11.1% 298 32992 9.6 U DIC1 SGDID:S000004340, Chr XII from 827872-826976, reverse complement, Verified ORF, "Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix" * Jamie_Ashy_SPB_8.02808.02808.4 2.2633 0.1498 17.4 1968.6565 1973.2609 4440.7 84 3.291 29.2 1 V.VNIRMQNDSALEAAKRR.N * Jamie_Ashy_SPB_32.11566.11566.2 1.8605 0.0529 80.2 1775.1721 1773.0973 6666.6 250 3.127 30.0 1 R.GILMTASQVVTYDVFK.N YJL157C 4 4 11.0% 830 94572 5.4 U FAR1 SGDID:S000003693, Chr X from 126245-123753, reverse complement, Verified ORF, "Cyclin-dependent kinase inhibitor that mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate" * Jamie_Ashy_SPB_62.22072.22072.4 3.0546 0.0825 14.4 4558.0166 4557.01 8182.6 250 3.118 14.0 1 R.NIKKCNSQFSPKRYIGEKCLICEESISSTFTGEKVVEST.C * Jamie_Ashy_SPB_34.12248.12248.2 1.5637 0.1051 13.2 1791.1921 1792.0012 7162.9 306 3.128 32.1 1 F.TGEFPQFTPQEQLIR.T * Jamie_Ashy_SPB_12.04357.04357.2 1.8305 0.0329 13.0 1156.5521 1156.1943 4609.2 380 3.296 56.2 1 H.VDHEELEER.E * Jamie_Ashy_SPB_91.32619.32619.4 2.7268 0.1481 15.7 2970.8965 2967.3574 5572.5 10 3.74 22.8 1 L.SHVNIIGALGAPPDVISAQSHDSRLPWK.R YML085C 2 2 11.0% 447 49800 5.1 U TUB1 SGDID:S000004550, Chr XIII from 99400-99376,99259-97941, reverse complement, Verified ORF, "Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules" * Jamie_Ashy_SPB_42.15080.15080.3 2.924 0.165 15.1 2053.4944 2052.3318 5982.7 64 3.808 30.6 1 K.SKLEFAVYPAPQVSTSVVE.P * Jamie_Ashy_SPB_76.27091.27091.4 2.9094 0.1448 89.9 3520.9365 3514.991 7082.6 417 3.102 20.7 1 R.DGKYMATCLLYRGDVVTRDVQRAVEQVKNK.K YLL010C 2 2 11.0% 427 47931 5.2 U PSR1 SGDID:S000003933, Chr XII from 130612-129329, reverse complement, Verified ORF, "Plasma membrane associated protein phosphatase involved in the general stress response; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p" * Jamie_Ashy_SPB_58.20854.20854.4 3.5058 0.1274 10.5 3768.1765 3767.0237 7326.4 155 3.263 18.1 1 S.NTKSRTRGVHQTNSPPSKTNSAATFSSTERSTGKSG.I * Jamie_Ashy_SPB_47.16742.16742.2 1.603 0.0673 77.4 1395.7122 1396.54 5410.7 84 3.351 45.0 1 K.RISKDDLYEEK.Y YOR004W 2 2 11.0% 254 28801 10.0 U YOR004W SGDID:S000005530, Chr XV from 333592-334356, Uncharacterized ORF, "Protein required for cell viability" * Jamie_Ashy_SPB_44.15837.15837.2 1.5374 0.054 69.0 1890.3722 1890.3694 8016.0 13 3.589 34.4 1 K.KRKLGPKAPNPLSVKKK.K * Jamie_Ashy_SPB_43.15296.15296.3 2.7239 0.0208 51.4 2975.4243 2974.5637 6709.6 40 3.168 21.2 1 K.RKLGPKAPNPLSVKKKKKVNSPSDEVK.D YJR056C 2 2 11.0% 236 27361 7.0 U YJR056C SGDID:S000003817, Chr X from 542416-541706, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_71.25537.25537.3 2.0181 0.0188 38.5 1892.3344 1896.0298 3579.4 69 2.873 33.3 1 R.NLNDITGLGHERERDR.E * Jamie_Ashy_SPB_20.07023.07023.2 1.6454 0.0048 66.2 1275.9922 1274.4191 5348.0 68 2.966 55.6 1 R.ENTHYEKKPK.L Reverse_YHR098C 4 4 10.9% 929 103950 6.3 U SFB3 SGDID:S000001140, Chr VIII from 301937-299148, reverse complement, Verified ORF, "Member of the Sec24p family; forms a complex, with Sec23p, that is involved in sorting of Pma1p into COPII vesicles; peripheral ER membrane protein; potential Cdc28p substrate" * Jamie_Ashy_SPB_16.05464.05464.2 1.6943 0.0349 10.7 1009.5522 1009.0704 3542.9 257 3.529 56.2 1 Q.EVSLGTSCR.V * Jamie_Ashy_SPB_24.08376.08376.3 2.5557 0.1526 16.1 3355.6743 3359.8188 9557.7 8 3.799 21.6 1 R.VKLLAQYGVINSVNTVMDNVLTFADTEQQF.H * Jamie_Ashy_SPB_98.35322.35322.3 2.3722 0.2035 12.7 3235.0144 3231.5078 8984.8 268 3.985 21.2 1 R.LHESEPINYMSLSMLHPDCSGQDVCYF.Q * Jamie_Ashy_SPB_93.33409.33409.4 3.176 0.1517 10.6 3706.4966 3706.116 8004.3 232 3.825 17.6 1 F.NHYARHPRRSKKSSAGGTHVTSEDLSLASVSAALI.N YPL179W 2 2 10.9% 549 61421 9.3 U PPQ1 SGDID:S000006100, Chr XVI from 208156-209805, Verified ORF, "Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors" * Jamie_Ashy_SPB_31.10962.10962.4 2.8054 0.1214 18.5 4583.7764 4579.1675 6184.8 6 3.282 16.2 1 P.IFLRRYSHDTSSNEGLDIDVAIEKLLQVGESREITKTSKK.K * Jamie_Ashy_SPB_101.36229.36229.2 1.829 0.1978 47.5 2314.112 2311.627 6654.4 3 4.251 34.2 1 I.IQDKIFCVHGGISPDLHDMK.Q Reverse_YPL093W 3 3 10.8% 647 74410 8.8 U NOG1 SGDID:S000006014, Chr XVI from 370975-372918, Verified ORF, "Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins" * Jamie_Ashy_SPB_109.39004.39004.3 1.2695 0.0621 33.7 4161.3843 4165.653 4071.3 3 3.272 10.0 1 K.ECAKNRVEMVNEELQCSSTMIEVGPVEKVSELLQAR.E * Jamie_Ashy_SPB_34.12130.12130.3 2.5512 0.0603 65.7 2552.2144 2553.133 8394.7 102 3.167 26.2 1 K.RMYFARIRTIKFGPRIVTPTK.R * Jamie_Ashy_SPB_71.25250.25250.2 1.5853 0.0817 12.7 1571.1322 1570.7892 6160.8 19 3.076 45.8 1 K.RQTRNLVIDLLDN.A YDR105C 2 2 10.8% 473 52230 5.5 U TMS1 SGDID:S000002512, Chr IV from 666765-665344, reverse complement, Verified ORF, "Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance" * Jamie_Ashy_SPB_28.09891.09891.2 1.7665 0.097 15.9 1459.6921 1460.6786 5060.9 72 3.465 45.5 1 V.RAALQNSWWSLK.F * Jamie_Ashy_SPB_45.16040.16040.4 3.0209 0.081 11.1 4211.6963 4215.592 4717.6 378 3.246 14.0 1 R.NQLRYEAIKQAVEEGSLPESALYDTAWLGTSSPTGAMDN.Q YDR148C 2 2 10.8% 463 50431 8.9 U KGD2 SGDID:S000002555, Chr IV from 755061-753670, reverse complement, Verified ORF, "Dihydrolipoyl transsuccinylase, a component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes a step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA" * Jamie_Ashy_SPB_75.26725.26725.3 2.5767 0.0135 45.0 3115.8245 3115.5999 6549.1 28 3.38 19.6 1 K.VESLGSIYLSGKKISVAANPFSITSNRFK.S * Jamie_Ashy_SPB_3.00792.00792.4 2.807 0.1068 18.4 2330.2966 2330.597 4965.6 154 3.475 27.5 1 M.AESLTEGSLKEYTKNVGDFIK.E YGR246C 3 3 10.7% 596 66907 6.9 U BRF1 SGDID:S000003478, Chr VII from 984277-982487, reverse complement, Verified ORF, "TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB" * Jamie_Ashy_SPB_102.36451.36451.4 2.5053 0.0734 65.6 3440.1765 3445.9065 3641.6 195 3.421 19.5 1 K.AAKLSVQKFRENDVEDGEARPPSFVKNRKK.E * Jamie_Ashy_SPB_82.29481.29481.2 1.556 0.0831 10.9 2331.9922 2332.6194 8487.3 29 3.119 27.5 1 E.QESKRLKQEADIATGNTSVKK.K * Jamie_Ashy_SPB_79.28323.28323.2 1.6255 0.078 12.8 1507.3922 1509.8505 5200.4 485 3.088 41.7 1 S.VKNMLQKASFSKK.I Reverse_YML118W 2 2 10.7% 505 57742 7.5 U NGL3 SGDID:S000004587, Chr XIII from 32334-33851, Verified ORF, "Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p" * Jamie_Ashy_SPB_88.31441.31441.4 3.821 0.2039 80.5 3749.9766 3746.3013 5731.3 4 4.093 19.6 1 K.GYGIGYLSVGKVHLSNHLAVLQNVLAKEEKTATFS.E * Jamie_Ashy_SPB_62.22172.22172.2 1.8335 0.0418 10.6 2324.8523 2327.2598 8656.7 8 3.49 33.3 1 G.RSKEDCEEDDQDSEEGNRK.K YLR180W 2 2 10.7% 382 41818 5.2 U SAM1 SGDID:S000004170, Chr XII from 515264-516412, Verified ORF, "S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)" Jamie_Ashy_SPB_22.07797.07797.2 1.582 0.0853 13.1 2088.8523 2089.436 8925.5 68 3.501 27.8 1 A.CETAAKTGMIMVFGEITTK.A * Jamie_Ashy_SPB_86.30684.30684.2 1.3631 0.1373 12.3 2571.412 2568.8438 9875.1 48 3.46 23.8 1 A.QHADEITTEDLRAQLKSEIIEK.V Reverse_YKR077W 2 2 10.7% 363 39862 10.1 U YKR077W SGDID:S000001785, Chr XI from 583295-584386, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_30.10806.10806.4 3.5588 0.0709 17.3 3033.5366 3030.3323 4084.0 325 3.517 22.4 1 R.APSRFQRPSSQLPLNFLFQNTNNASGH.N * Jamie_Ashy_SPB_62.22272.22272.2 1.6171 0.0548 73.5 1415.5322 1412.5889 4766.3 53 3.194 45.5 1 K.SYKKNVTSSKNR.M contaminant_NRL_1IKFL 2 2 10.7% 214 23527 6.5 U owl|| Immunoglobulin igg1-kappa antibody fragment fab complexed With... contaminant_S68241 2 2 10.6% 218 23846 6.7 U owl|S68241| immunoglobulin light chain (Mab13-1) - mouse (fragment) Jamie_Ashy_SPB_15.05128.05128.2 2.014 0.0904 17.8 1458.1522 1456.5927 4994.5 1 3.37 68.2 1 S.STLTLTKDEYER.H Jamie_Ashy_SPB_14.04926.04926.4 3.4331 0.2024 65.0 2786.6165 2785.9502 6078.5 1 3.904 31.1 1 S.STLTLTKDEYERHNSYTCEATHK.T Reverse_YIL066C 2 2 10.6% 869 97515 6.0 U RNR3 SGDID:S000001328, Chr IX from 240705-238096, reverse complement, Verified ORF, "Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits" * Jamie_Ashy_SPB_39.13858.13858.4 2.6919 0.1281 18.9 6007.8164 6003.8477 7054.2 54 3.498 12.3 1 E.SFKTGKPVYVPRDLESVSAIHTAAQDAVEQDITFQIAASAAQTRLYYMGTKLGKK.W * Jamie_Ashy_SPB_39.13825.13825.4 2.9219 0.1102 18.1 4101.6963 4104.503 6612.5 148 3.302 15.3 1 K.ANWMDFQLIGKSAPSGEFTSYKGEKQALECSAELTAH.Y Reverse_YPR048W 3 3 10.6% 623 72328 8.5 U TAH18 SGDID:S000006252, Chr XVI from 659179-661050, Verified ORF, "Protein with a potential role in DNA replication; displays synthetic lethal genetic interaction with the pol3-13 allele of POL3, which encodes DNA polymerase delta" * Jamie_Ashy_SPB_66.23623.23623.3 2.8823 0.1506 10.5 3097.9744 3100.9011 6351.5 13 3.78 22.4 1 F.LLKIDKSIEAKVVSLLPALGVGPGVLIMPK.N * Jamie_Ashy_SPB_77.27488.27488.4 3.2573 0.1699 10.7 4221.177 4222.6953 8390.3 46 3.587 17.1 1 I.VKTSTFKENAHEDSLQLYSAPELYVEPDLEERKIIQ.G * Jamie_Ashy_SPB_11.03782.03782.3 2.4459 0.1122 18.2 1408.7043 1406.4485 5381.0 254 3.46 36.4 1 K.ENAHEDSLQLYS.A Reverse_YKL082C 2 2 10.6% 434 50482 9.6 U RRP14 SGDID:S000001565, Chr XI from 281973-280669, reverse complement, Uncharacterized ORF, "Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family" * Jamie_Ashy_SPB_6.01940.01940.1 1.5581 0.0159 83.7 974.58 974.15137 4977.8 7 2.79 64.3 1 R.KKGKNDKR.I * Jamie_Ashy_SPB_70.25180.25180.4 3.3803 0.0848 16.2 4555.4565 4550.0977 9934.1 25 3.694 15.3 1 K.MVELTSSTEDDRQEPDLKKLKDNKSQEKTKKKAKWQEQ.S contaminant_KERATIN11 2 2 10.6% 424 48487 5.7 U no description * Jamie_Ashy_SPB_25.08848.08848.2 1.9907 0.0954 17.6 1957.7522 1960.2003 6788.9 18 2.975 41.2 1 R.RSFHRSLSSSLQAPVVST.V * Jamie_Ashy_SPB_30.10672.10672.3 2.2928 0.0344 43.1 3113.3643 3115.529 5250.3 102 3.121 20.2 1 K.GTEVQLTELRRTSQSLEIELQSHLSMK.E YPL048W 2 2 10.6% 415 47088 8.4 U CAM1 SGDID:S000005969, Chr XVI from 464398-465645, Verified ORF, "Translational cofactor elongation factor-1 gamma, participates in the regulation of GTP-binding protein EF-1 alpha, may play a redundant role in the regulation of protein synthesis or another GTP-dependent process" * Jamie_Ashy_SPB_6.01863.01863.2 1.534 0.0308 66.2 1183.5521 1184.5082 5312.3 40 3.604 55.0 1 R.GLVKALKLDVK.V * Jamie_Ashy_SPB_77.27576.27576.4 3.1244 0.1156 14.8 3906.0566 3910.5515 7490.7 271 3.367 16.7 1 F.NTVRASPFLKDEYKDFKFADKPLSPPQKKKEKK.A Reverse_YNL264C 2 2 10.6% 350 40678 7.2 U PDR17 SGDID:S000005208, Chr XIV from 146616-145564, reverse complement, Verified ORF, "Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition" * Jamie_Ashy_SPB_19.06829.06829.4 3.5965 0.1923 65.1 2566.6165 2564.0203 5765.0 2 4.48 27.2 1 R.KLRLDDVKKVMDPWYVEHKY.K * Jamie_Ashy_SPB_28.09921.09921.2 1.4556 0.0414 63.9 2004.7122 2005.2909 6019.3 25 3.493 37.5 1 K.DCPILKEKPVAPTHDRK.R Reverse_YCL024W 5 5 10.5% 1037 116475 8.7 U KCC4 SGDID:S000000529, Chr III from 79161-82274, Verified ORF, "Protein kinase of the bud neck involved in the septin checkpoint, associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p" * Jamie_Ashy_SPB_87.31342.31342.3 2.9914 0.2094 49.4 2248.5842 2252.5784 4313.2 2 4.184 28.8 1 E.RQYLGLGSANKGQFSSLSPLK.V * Jamie_Ashy_SPB_45.16066.16066.2 1.9536 0.0387 13.5 1228.1721 1225.4337 4791.5 3 3.383 60.0 1 P.TLGPDLRSIPR.T * Jamie_Ashy_SPB_15.05204.05204.3 2.5662 0.0179 45.7 2900.9343 2897.2175 5546.1 202 3.322 20.0 1 R.LPIPSSDTPNSSLLFSSRKRSSSFER.S * Jamie_Ashy_SPB_57.20539.20539.4 2.6442 0.0052 43.9 3630.1365 3630.065 5801.4 55 2.828 18.8 1 R.SHRKSTRRRPTSQISRNNLLLSSSSSLTSSRK.N * Jamie_Ashy_SPB_39.13882.13882.4 2.7884 0.0485 72.4 2341.0964 2336.8728 4749.9 339 3.091 29.6 1 K.ITQYKKLLPHSLIDRIKIR.Q Reverse_YPL147W 4 4 10.5% 870 100025 9.5 U PXA1 SGDID:S000006068, Chr XVI from 273254-275866, Verified ORF, "Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins" * Jamie_Ashy_SPB_9.03224.03224.4 3.0562 0.1334 15.0 1871.2166 1869.1283 5343.1 329 3.968 30.2 1 I.GESARVSPIIVDINELR.I * Jamie_Ashy_SPB_16.05536.05536.2 1.4903 0.1222 82.4 2068.8123 2070.3994 6154.1 128 3.424 32.4 1 R.VSPIIVDINELRIGNFNR.Q * Jamie_Ashy_SPB_45.16137.16137.4 2.1538 0.0212 38.3 5503.8564 5504.5884 8774.9 248 3.34 12.9 1 R.NRRKWPFKYFKYCKLIIKFTSYTVGSFLTVGSLFLSVLLIQARRR.L * Jamie_Ashy_SPB_80.28514.28514.3 2.8324 0.1713 13.3 3035.2144 3031.67 8142.5 59 3.952 20.8 1 R.RRLKPHAANSRSIQLLHRILLKFYV.K Reverse_YDR295C 2 2 10.5% 674 77059 8.7 U HDA2 SGDID:S000002703, Chr IV from 1054641-1052617, reverse complement, Verified ORF, "Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda3p; Ploidy-related" * Jamie_Ashy_SPB_48.17274.17274.4 3.1143 0.1222 89.1 4026.8965 4023.5327 8156.2 481 3.621 15.7 1 R.AKLSQFDNQLRELEKKLSETSFKTLPGNVENELSK.M * Jamie_Ashy_SPB_26.09385.09385.4 3.8109 0.1008 15.4 4094.9766 4091.527 9943.4 116 3.394 16.2 1 K.LKDALASGEELHNESSITNSPLKNFNELEQRNKLLH.N YHR001W 2 2 10.5% 437 49805 7.2 U OSH7 SGDID:S000001043, Chr VIII from 106050-107363, Verified ORF, "Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability" * Jamie_Ashy_SPB_76.27156.27156.4 3.3136 0.0904 15.8 4247.1367 4244.6543 6094.2 6 3.417 16.7 1 K.YQVDIEFKTKGFISGTYDAIEGTIKDYDGKEYYQISG.K * Jamie_Ashy_SPB_11.03958.03958.2 1.6245 0.0437 10.0 1125.2722 1127.1094 5179.9 2 3.212 68.8 1 L.EEQGEYESR.R Reverse_YJL145W 2 2 10.5% 294 34388 7.9 U SFH5 SGDID:S000003681, Chr X from 145078-145962, Verified ORF, "Putative phosphatidylinositol transfer protein (PITP), exhibits phosphatidylinositol- but not phosphatidylcholine-transfer activity, mainly localized to cytosol and microsomes, similar to Sec14p; may be PITP regulator rather than actual PITP" * Jamie_Ashy_SPB_26.09363.09363.2 1.5495 0.0222 63.8 1387.4521 1389.4272 4319.6 34 2.95 50.0 1 K.DKGGYGEYPCDK.L * Jamie_Ashy_SPB_19.06858.06858.4 3.3392 0.1258 10.0 2654.1765 2656.0637 4973.5 143 3.936 24.2 1 Y.PCDKLYQGLKSGDTLVVFKKRTT.E YBR256C 2 2 10.5% 238 26196 5.2 U RIB5 SGDID:S000000460, Chr II from 728097-727381, reverse complement, Verified ORF, "Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway" * Jamie_Ashy_SPB_5.01839.01839.2 2.0948 0.0231 15.6 1016.5122 1016.18866 5332.5 45 2.798 62.5 1 D.TVANIVSRR.P * Jamie_Ashy_SPB_82.29390.29390.2 1.9965 0.1029 10.4 1780.9122 1779.1022 6591.0 1 4.084 43.3 1 Q.ISKKDSTLNTMISNII.E YEL053C 2 2 10.4% 733 84302 5.9 U MAK10 SGDID:S000000779, Chr V from 56102-53901, reverse complement, Verified ORF, "Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible" * Jamie_Ashy_SPB_50.17712.17712.4 3.1618 0.0727 12.9 4330.9766 4329.142 8011.4 237 2.966 15.2 1 T.YQFAKFFNKLEQRHVIARILFPLFFIRDDRTVLGK.F * Jamie_Ashy_SPB_40.14377.14377.4 2.9996 0.2314 59.2 4620.6567 4620.443 8906.3 2 4.321 15.8 1 N.GVLVTGTRLVQELSLEYYCKLKHTSKALSVNSKVIVNTLKK.N Reverse_YJL012C 3 3 10.4% 721 83155 6.8 U VTC4 SGDID:S000003549, Chr X from 413314-411149, reverse complement, Verified ORF, "Vacuolar membrane protein involved in vacuolar polyphosphate accumulation; functions as a regulator of vacuolar H+-ATPase activity and vacuolar transporter chaperones; involved in non-autophagic vacuolar fusion" * Jamie_Ashy_SPB_100.35781.35781.4 3.1968 0.1427 10.2 3132.0166 3127.6104 4302.9 357 3.882 19.9 1 S.GVLERVWEPPEQGLQTQLKVELVAYPF.R * Jamie_Ashy_SPB_41.14637.14637.4 3.9723 0.2019 83.8 4105.2563 4109.98 7526.4 40 3.88 18.6 1 R.LKKKLMVYQIESALAELNEIENMPKKGEKRMKAFV.Q * Jamie_Ashy_SPB_50.17709.17709.2 1.7884 0.0961 15.8 1564.7322 1565.7845 5500.6 1 3.245 58.3 1 R.HTKREVFITDTSM.G YOR350C 3 3 10.4% 663 77230 8.8 U MNE1 SGDID:S000005877, Chr XV from 994850-992859, reverse complement, Verified ORF, "Mitochondrial protein similar to Lucilia illustris mitochondrial cytochrome oxidase" * Jamie_Ashy_SPB_92.33000.33000.3 1.9814 0.0277 35.6 2571.1143 2572.0232 5908.5 14 3.21 26.1 1 K.LIKDSLITPEILPQLGRQPSSHK.R * Jamie_Ashy_SPB_53.18806.18806.4 3.0565 0.1258 12.3 3352.2166 3355.9517 7918.4 24 3.914 20.7 1 V.VLTTLQKLRTSDNPALYFALLNRIGTGHIK.W * Jamie_Ashy_SPB_54.19461.19461.2 1.6078 0.0692 10.0 2002.9922 2005.3052 6805.6 346 2.929 30.0 1 G.YQFTSHDFLSMLKVCK.N Reverse_YOR239W 2 2 10.4% 628 71486 4.7 U ABP140 SGDID:S000005765, Chr XV from 784857-785687,785689-786744, Verified ORF, "Nonessential protein that binds actin filaments and localizes to actin patches and cables, has similarity to S-adenosylmethionine (AdoMet)-dependent methyltransferases" * Jamie_Ashy_SPB_80.28508.28508.4 3.2245 0.2014 97.0 4334.8564 4336.8555 6334.8 1 3.875 19.2 1 R.DKFFNEKNNKYFIDWYRAPNENYLKKDFEPVPHK.F * Jamie_Ashy_SPB_59.21075.21075.3 3.1713 0.1538 10.2 3367.4644 3371.429 7723.6 242 3.795 17.5 1 L.DRGIRSGETDSKEFDSKEVANQDGTNDGRGE.H YPR018W 2 2 10.4% 606 70210 6.7 U RLF2 SGDID:S000006222, Chr XVI from 594473-596293, Verified ORF, "Largest subunit (p90) of the Chromatin Assembly Complex (CAF-I) with Cac2p and Msi1p that assembles newly synthesized histones onto recently replicated DNA; involved in the maintenance of transcriptionally silent chromatin" * Jamie_Ashy_SPB_52.18489.18489.2 2.4094 0.1715 66.7 2986.5923 2988.2969 5580.5 4 4.835 26.9 1 D.RNTNKQVPNGNIIAIETKSRSSSPCSK.R * Jamie_Ashy_SPB_67.24122.24122.4 3.087 0.0834 15.8 4250.6567 4245.9614 6508.9 278 3.055 15.7 1 A.QKNLPQYNKQTIKNTIKEYAIRSSGKGDLPRKWVIK.D Reverse_YFL002C 4 4 10.4% 606 69422 9.0 U SPB4 SGDID:S000001894, Chr VI from 146929-145109, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients" * Jamie_Ashy_SPB_33.11718.11718.2 2.4862 0.1388 57.3 1916.4922 1917.0801 7114.1 19 3.958 40.0 1 A.NEDLEEAKLEEEIAKR.K * Jamie_Ashy_SPB_72.25845.25845.2 1.8269 0.023 10.1 1451.6322 1452.7802 4724.4 40 2.78 50.0 1 K.KFKYNNLISVLL.Q * Jamie_Ashy_SPB_6.01879.01879.2 1.6757 0.2059 97.4 1322.0721 1322.5533 4950.5 4 4.56 50.0 1 R.GPTGILIQPRNR.L * Jamie_Ashy_SPB_55.19768.19768.2 1.6198 0.0862 12.7 2319.372 2316.7434 7060.3 210 2.965 22.7 1 K.VVKELVPIVFAATKGSGTVSDVV.V Reverse_YJR049C 2 2 10.4% 530 59469 7.2 U UTR1 SGDID:S000003810, Chr X from 528390-526798, reverse complement, Verified ORF, "NAD kinase, active as a hexamer; enhances the activity of ferric reductase (Fre1p)" * Jamie_Ashy_SPB_35.12426.12426.4 3.0308 0.111 16.0 2771.1765 2771.1516 6203.6 276 3.57 21.7 1 E.VTPFAYPSACITIFDGKQLEIRDK.G * Jamie_Ashy_SPB_100.35940.35940.4 3.4725 0.1209 15.0 3548.0964 3553.9297 6076.3 11 3.76 22.2 1 S.SDKNLLSSRRSSSESSIKRLMEKANDIDQTR.D YGR285C 2 2 10.4% 433 49020 8.3 U ZUO1 SGDID:S000003517, Chr VII from 1063159-1061858, reverse complement, Verified ORF, "Cytosolic ribosome-associated chaperone, contains a DnaJ domain; together with Ssz1p, acts as a chaperone for nascent polypeptide chains" * Jamie_Ashy_SPB_30.10648.10648.3 2.8924 0.1354 14.1 2866.9143 2865.218 7151.4 8 3.576 24.0 1 R.KQVVKYHPDKQSAAGGSLDQDGFFKI.I * Jamie_Ashy_SPB_11.03661.03661.2 1.2088 0.1784 13.1 1914.5322 1916.182 5294.5 271 3.973 25.0 1 K.ANAAGAKEVLKESAKTIVD.S Reverse_YLR309C 4 4 10.3% 911 105225 5.6 U IMH1 SGDID:S000004300, Chr XII from 751771-749036, reverse complement, Verified ORF, "Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi" * Jamie_Ashy_SPB_23.08050.08050.2 2.1668 0.032 17.3 1459.0922 1459.692 7062.0 158 3.151 46.2 1 D.GTTIGGKNKKGKNR.N * Jamie_Ashy_SPB_83.29847.29847.4 2.184 0.0408 42.2 5247.0566 5249.9155 6451.6 38 3.443 12.9 1 K.REKEKLTINLEALESSKEEMITSKSSLNQELETIKMRSDELSHNK.I * Jamie_Ashy_SPB_18.06313.06313.3 2.6275 0.0242 55.6 2158.3743 2161.4172 7006.0 103 3.534 25.0 1 K.LESISVEEKQKKAEREEK.L * Jamie_Ashy_SPB_16.05786.05786.2 1.6064 0.1175 17.7 1903.7922 1905.0721 6498.5 61 3.072 34.4 1 T.ESSASEPIESNQKTLRK.I Reverse_YHR084W 2 2 10.3% 688 77867 5.7 U STE12 SGDID:S000001126, Chr VIII from 274176-276242, Verified ORF, "Transcription factor that is activated by a MAP kinase signaling cascade, activates genes involved in mating or pseudohyphal/invasive growth pathways; cooperates with Tec1p transcription factor to regulate genes specific for invasive growth" * Jamie_Ashy_SPB_75.26946.26946.3 2.344 0.1627 18.3 3797.3643 3797.0125 9019.6 56 3.844 17.1 1 A.GGEESQVVLTNSDPTPMSIEKNKDPHASDEDGASPR.A * Jamie_Ashy_SPB_117.42076.42076.4 3.1874 0.1389 12.3 3776.8965 3777.43 7380.9 4 3.74 21.6 1 T.KIHLPKSIKASKGVSQKNGTPVNGQSSKMGVTRRR.P YNL012W 2 2 10.3% 631 72187 8.2 U SPO1 SGDID:S000004957, Chr XIV from 609687-609791,609876-611666, Verified ORF, "Meiosis-specific protein with similarity to phospholipase B, required for meiotic spindle pole body duplication and separation; required for spore formation" * Jamie_Ashy_SPB_27.09502.09502.4 1.8644 0.071 39.4 5754.0566 5759.639 6142.5 25 2.733 11.8 1 K.CINNFDDLGFITATSSSIFNNVLIFIWNLASQSSREAMKALNMVMGIFGLGK.E * Jamie_Ashy_SPB_44.15811.15811.2 1.8574 0.0397 12.9 1533.4122 1532.7826 7061.0 3 3.546 54.2 1 K.RTGHDSFLPIIIY.H YDR068W 2 2 10.3% 310 35964 4.6 U DOS2 SGDID:S000002475, Chr IV from 583710-584642, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * Jamie_Ashy_SPB_79.28396.28396.2 1.5026 0.047 65.8 2186.132 2188.4026 6663.1 52 2.938 31.6 1 K.ENEIAVGGNRTEAELRTLSK.D * Jamie_Ashy_SPB_61.21908.21908.2 1.6762 0.0941 16.9 1291.7322 1289.4717 4204.8 131 3.906 40.9 1 G.ETKVAVSQEGLK.D YMR108W 3 3 10.2% 687 74937 8.5 U ILV2 SGDID:S000004714, Chr XIII from 484083-486146, Verified ORF, "Acetolactate synthase, catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control" * Jamie_Ashy_SPB_15.05305.05305.2 2.123 0.0018 10.8 1523.8522 1524.8038 3655.3 311 2.998 39.3 1 N.LAVQNADLIIAVGAR.F * Jamie_Ashy_SPB_92.33016.33016.3 2.8218 0.1404 12.4 2353.3145 2351.825 5413.4 158 3.719 25.0 1 Q.IAVEGDATTNLGKMMSKIFPVK.E * Jamie_Ashy_SPB_63.22483.22483.3 1.7668 0.0022 33.7 3740.9343 3744.2976 9712.3 399 2.858 16.4 1 K.EYPYAYMEETPGSKIKPQTVIKKLSKVANDTGR.H YNL176C 3 3 10.2% 636 67357 5.6 U YNL176C SGDID:S000005120, Chr XIV from 306982-305072, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_18.06516.06516.3 2.2493 0.2289 10.0 2914.2844 2910.9417 5806.7 6 4.244 25.0 1 D.TTCPSSSRNTSTSFYSSSLSSTNDFST.I * Jamie_Ashy_SPB_77.27433.27433.4 3.2503 0.0631 10.9 3173.6565 3175.853 6983.7 72 3.87 21.8 1 T.NTGTIVGSVVGSVGGILICVLVVWFMLVRK.R * Jamie_Ashy_SPB_4.01147.01147.2 2.3487 0.0524 28.7 1054.4321 1054.1223 5604.7 89 2.8 71.4 1 S.NFMDQENR.F Reverse_YLR394W 2 2 10.2% 482 53863 10.2 U CST9 SGDID:S000004386, Chr XII from 907950-909398, Verified ORF, "Protein required for synaptonemal complex formation, may have a role in meiotic recombination; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate" * Jamie_Ashy_SPB_22.07912.07912.2 1.6984 0.0912 15.9 1047.3121 1046.1686 4685.4 19 3.559 61.1 1 S.SPQLSASSLR.S * Jamie_Ashy_SPB_61.21673.21673.4 3.7094 0.0747 14.4 4453.4565 4451.972 8160.6 458 3.413 14.9 1 K.SEVLNIISIDSSRCIPCVKSTLPSHQSCLIHACSTLRLP.D YDR494W 2 2 10.2% 361 41216 10.0 U RSM28 SGDID:S000002902, Chr IV from 1436920-1438005, Verified ORF, "Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation" * Jamie_Ashy_SPB_56.20119.20119.2 1.7527 0.0778 13.3 2305.2322 2304.7373 7477.3 202 3.024 25.0 1 S.SNALKLDKMKEGRMQNKRR.N * Jamie_Ashy_SPB_26.09084.09084.2 1.8183 0.097 14.6 2033.4521 2033.2236 8057.9 15 3.463 35.3 1 K.KMQNGKNNLKGDARVEDD.L YLR283W 2 2 10.2% 314 36574 7.7 U YLR283W SGDID:S000004273, Chr XII from 705188-706132, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_98.35076.35076.2 1.2783 0.0358 61.5 2372.2522 2374.6536 9476.5 42 3.232 25.0 1 K.NIQLSDLSSSPLSKNKEKQEK.P * Jamie_Ashy_SPB_36.12752.12752.2 1.6813 0.1 16.0 1284.2522 1285.573 7658.7 136 2.925 50.0 1 N.KIQTKILGDIR.S YPR104C 3 3 10.1% 936 103502 5.9 U FHL1 SGDID:S000006308, Chr XVI from 736431-733621, reverse complement, Verified ORF, "Putative transcriptional regulator with similarity to DNA-binding domain of Drosophila forkhead; required for rRNA processing" * Jamie_Ashy_SPB_84.30124.30124.4 2.6769 0.1966 10.9 2866.6965 2871.056 4078.4 283 3.595 21.8 1 G.EMAIIESSNHVGTSSPTTETQFTIDSS.A * Jamie_Ashy_SPB_29.10234.10234.3 2.3189 0.1806 15.9 3990.2344 3986.4397 7855.1 79 4.495 16.2 1 S.SLSRFFQPRQNARATSSVAATSVPAAASQNVDAQPKPK.P * Jamie_Ashy_SPB_84.30076.30076.4 2.8525 0.1231 17.4 3447.3364 3441.7336 6501.4 214 3.243 20.1 1 D.EEGHDSKRRKVSEDINEGITEVNVSLEEKL.- Reverse_YPL268W 3 3 10.1% 869 100547 9.1 U PLC1 SGDID:S000006189, Chr XVI from 35236-37845, Verified ORF, "Phosphoinositide-specific phospholipase C, hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in kinetochore function and pseudohyphal differentiation" * Jamie_Ashy_SPB_103.36907.36907.4 2.7614 0.1523 11.5 3710.9365 3712.065 5045.0 422 3.216 17.6 1 D.TDKLTISVEADWIPNFGNGQSSKTSAETASIRTGK.D * Jamie_Ashy_SPB_60.21358.21358.3 2.799 0.1233 18.0 3110.9944 3114.476 7900.7 374 3.425 18.5 1 K.LGIRKIRQMSSSNTASSSNTSDDKMRLE.Q * Jamie_Ashy_SPB_33.11833.11833.3 2.7064 0.1532 13.5 2977.6443 2980.4023 6957.1 39 3.563 22.9 1 K.SYLEEKVEVLYPQEKLFKTFGDQGM.T Reverse_YER070W 2 2 10.1% 888 99561 6.2 U RNR1 SGDID:S000000872, Chr V from 298948-301614, Verified ORF, "Ribonucleotide-diphosphate reductase (RNR), large subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits" * Jamie_Ashy_SPB_43.15422.15422.4 3.2359 0.0709 15.3 3416.0166 3420.729 8520.2 28 3.391 18.7 1 E.SSSDLSPITANATVAAPVFDSAAYSASSPMYSPR.K * Jamie_Ashy_SPB_105.37585.37585.4 1.395 0.0184 33.4 6539.2163 6534.64 4277.0 107 3.478 6.4 1 K.LGKKWGYFHMSTLKGMTPARLFLNLSHSQDIYVSRDAAMNIITKQSIEWVTKYLDK.L YER041W 2 2 10.1% 759 87390 7.2 U YEN1 SGDID:S000000843, Chr V from 232460-234739, Verified ORF, "Protein of unknown function, has similarity to endonuclease Rth1p; potentially phosphorylated by Cdc28p" * Jamie_Ashy_SPB_33.11889.11889.4 2.6855 0.1327 18.1 5001.7363 5005.279 7527.4 1 3.854 14.8 1 E.DTSLEEIPAPTRLTTVDEHSDNDDDSLIFVDEITNSQSVLDSSPGK.R * Jamie_Ashy_SPB_111.39993.39993.2 1.1652 0.1999 84.8 3345.9722 3343.725 3089.7 2 4.17 13.3 1 K.SRTTNAEKNPPESGLKSRSSITINARLQGTK.M Reverse_YNL045W 2 2 10.1% 671 77353 6.6 U YNL045W SGDID:S000004990, Chr XIV from 542964-544979, Verified ORF, "Leucyl aminopeptidase with epoxide hydrolase activity, metalloenzyme containing one zinc atom; role in vivo is not defined" * Jamie_Ashy_SPB_90.32175.32175.3 1.9813 0.1873 11.5 4453.5845 4452.006 7054.7 24 3.878 16.2 1 F.TMNPSEMGGYPYSDVNVLIDYTGWEYEFIIKEATQIFK.E * Jamie_Ashy_SPB_2.00694.00694.3 1.1118 0.0399 32.6 3280.7344 3283.749 2173.3 109 2.894 8.6 1 K.KLQFTVSGSIASKEFSVSLNLDTHLVAFDK.Y Reverse_contaminant_GR78_LYCES 2 2 10.1% 666 73235 5.2 U owl|P49118| 78 KD GLUCOSE REGULATED PROTEIN HOMOLOG PRECURSOR (GRP 78) (IMMUNOGLOBULIN... * Jamie_Ashy_SPB_84.30090.30090.3 2.8211 0.2543 62.9 3058.5244 3060.4001 8111.1 36 4.169 20.7 1 E.ESAGGGPAGGSRQYVATIIPNCVAEVEKLK.E * Jamie_Ashy_SPB_51.18092.18092.4 3.3612 0.0631 81.5 4301.6167 4303.7417 7704.3 39 3.445 15.3 1 R.ARTLPESFDTGDFLSEIEVRVQHQSSLSRKAREAERR.L Reverse_YFL049W 3 3 10.1% 623 70276 8.7 U YFL049W SGDID:S000001845, Chr VI from 36803-38674, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_52.18621.18621.4 3.6981 0.1995 63.0 4217.6567 4221.803 6825.7 205 4.114 15.8 1 T.AVVPNANTNKVETDLKVATKDGAVNQPVKNVHSYIYPPK.F * Jamie_Ashy_SPB_12.04222.04222.2 1.9996 0.0591 15.7 1782.0922 1783.0806 5553.8 3 3.017 50.0 1 K.LYKNKFEDLQSRKL.G * Jamie_Ashy_SPB_36.12728.12728.2 1.4449 0.0171 63.5 1158.1522 1157.2255 5171.8 254 3.049 50.0 1 K.YSTNTADRTK.L YNL073W 2 2 10.1% 576 66128 8.9 U MSK1 SGDID:S000005017, Chr XIV from 488387-490117, Verified ORF, "Mitochondrial lysine-tRNA synthetase, required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria" * Jamie_Ashy_SPB_62.22042.22042.2 1.539 0.0331 65.9 1406.2722 1403.6427 6028.2 32 3.055 54.5 1 K.MEATRRNGQIVK.D * Jamie_Ashy_SPB_87.31237.31237.4 2.6553 0.1903 11.3 5231.2563 5235.0347 7138.1 3 3.854 13.7 1 L.QLQKFLQQKQINELTGNKTETLSPVIDYQYVETMKYGMPPVGGFGL.G Reverse_YDR188W 2 2 10.1% 546 59924 5.9 U CCT6 SGDID:S000002596, Chr IV from 836416-838056, Verified ORF, "Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif" * Jamie_Ashy_SPB_63.22548.22548.4 2.208 0.041 42.4 3431.1365 3426.0635 6417.2 383 2.687 17.2 1 K.TKGKAGLKNMNASRLYRSLAIYFAGAGPIINK.D * Jamie_Ashy_SPB_49.17504.17504.4 2.8782 0.0873 10.8 2472.6565 2477.8115 4304.8 10 3.496 28.0 1 A.DYVSLVADTVIPTLVETLDADVK.T YKL172W 2 2 10.1% 427 49734 6.4 U EBP2 SGDID:S000001655, Chr XI from 125764-127047, Verified ORF, "Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles" * Jamie_Ashy_SPB_52.18529.18529.2 1.5802 0.0359 69.0 1379.0922 1377.6322 4413.9 9 3.264 50.0 1 K.HALERVQLPWK.K * Jamie_Ashy_SPB_61.21726.21726.4 3.2912 0.0989 15.7 3892.9365 3891.1096 6270.2 27 3.388 18.3 1 S.FQEHQSVTSETNTDEHIKDIYDDTERELAFYK.Q YKL050C 4 4 10.0% 922 103144 6.1 U YKL050C SGDID:S000001533, Chr XI from 345264-342496, reverse complement, Uncharacterized ORF, "Putative protein of unknown function" * Jamie_Ashy_SPB_29.10467.10467.2 1.8893 0.0175 12.5 1202.9722 1203.3378 5269.0 91 3.122 60.0 1 L.DLSKVLDGAER.R * Jamie_Ashy_SPB_97.34780.34780.2 1.6305 0.1676 22.0 2010.5922 2010.171 6793.0 14 3.552 33.3 1 V.NFKNGLQSSDQSGVSKANK.E * Jamie_Ashy_SPB_73.26005.26005.4 2.6147 0.0709 67.6 5020.8164 5024.4883 7782.4 25 3.804 14.3 1 K.LEEEKETAKRSRRGTEESKNNSNFSRDVIMSTPDNNEKVTPIGK.S * Jamie_Ashy_SPB_100.35766.35766.2 2.2654 0.28 50.0 1936.9122 1934.0697 5344.2 131 4.993 29.4 1 S.RFLSTYNTGKDIDSSASA.R YIL091C 3 3 10.0% 721 83991 6.3 U YIL091C SGDID:S000001353, Chr IX from 193192-191027, reverse complement, Uncharacterized ORF, "Protein required for cell viability" * Jamie_Ashy_SPB_20.06991.06991.3 2.402 0.0461 54.3 2111.6042 2111.49 6995.7 237 3.189 25.0 1 K.VQNGLLDKKTDPLTALQKK.L * Jamie_Ashy_SPB_33.11824.11824.4 2.8513 0.0018 51.3 2624.1765 2624.789 6405.4 16 3.205 26.2 1 K.NNQRLQDNPDTEHLDQGFTRPK.V * Jamie_Ashy_SPB_71.25416.25416.4 2.9881 0.0996 17.7 3591.0166 3595.1875 7742.6 6 3.28 20.6 1 D.SLPPKSKPKSFQHIFRGNTNDFFVVGLKFTR.K YIR003W 3 3 10.0% 679 74763 5.0 U YIR003W SGDID:S000001442, Chr IX from 360882-362921, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_18.06324.06324.2 1.9722 0.0743 90.3 1290.6322 1290.4534 5184.9 168 3.625 55.0 1 K.ELEEIESLISK.H * Jamie_Ashy_SPB_47.16873.16873.4 2.5272 0.165 15.6 5290.5366 5296.5054 7325.1 104 4.111 12.5 1 N.KTSTNPSPLEKNEHEGAEGNESAISPSNLVNKSNNEVTEHSDSEDLTEK.Q * Jamie_Ashy_SPB_2.00655.00655.2 1.5793 0.0649 79.7 862.4322 862.06287 4342.7 76 3.604 64.3 1 R.RAPPPVPK.K Reverse_contaminant_KERATIN19 2 2 10.0% 468 51203 5.5 U no description * Jamie_Ashy_SPB_56.19982.19982.2 1.73 0.1969 33.8 1597.4521 1598.8085 6498.4 51 3.757 38.5 1 A.ELEVKSMYAADVDK.K * Jamie_Ashy_SPB_56.19988.19988.4 2.5442 0.0162 44.2 3185.9365 3182.5264 7088.4 181 2.797 18.8 1 R.SAGLGYLSSSGLGGPRASSLRVQAGRGSFAASR.S YNL027W 2 2 9.9% 678 76301 6.1 U CRZ1 SGDID:S000004972, Chr XIV from 579581-581617, Verified ORF, "Transcription factor that activates transcription of genes involved in stress response; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation" * Jamie_Ashy_SPB_72.25844.25844.4 2.4779 0.1259 79.0 4924.177 4925.5293 9785.0 139 3.287 13.5 1 R.KLKLKKSRRRSSQTSNNSFTSRRSSRSRSISPDEKAKSISANR.E * Jamie_Ashy_SPB_46.16580.16580.4 3.2679 0.0822 17.7 2876.6965 2881.242 4729.3 466 3.093 22.5 1 N.PANFACDVCGKKFTRPYNLKSHLR.T Reverse_YIL155C 2 2 9.9% 649 72389 7.9 U GUT2 SGDID:S000001417, Chr IX from 53708-51759, reverse complement, Verified ORF, "Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner" * Jamie_Ashy_SPB_91.32610.32610.4 3.4145 0.1288 10.6 3719.5764 3723.276 6568.6 35 3.82 18.1 1 D.STFLFHSRVVGQTASGKKGDAPITRPDRVLPRVGA.W * Jamie_Ashy_SPB_29.10244.10244.3 2.7294 0.1996 93.4 2944.4944 2948.3672 6414.9 3 4.482 23.2 1 R.VGGHIMKTSKSSTGSAFDGKEVLAVNLGR.T Reverse_YLR099C 2 2 9.9% 394 45141 9.1 U ICT1 SGDID:S000004089, Chr XII from 340929-339745, reverse complement, Verified ORF, "Protein of unknown function, null mutation leads to an increase in sensitivity to Calcofluor white; expression of the gene is induced in the presence of isooctane" * Jamie_Ashy_SPB_115.41245.41245.4 3.1637 0.1455 10.4 3680.2566 3683.1987 6906.2 4 3.68 21.5 1 I.TQPQVTQNKGVFSHLLYDKYIQDPVKVYATSI.Y * Jamie_Ashy_SPB_3.01123.01123.2 1.8074 0.0090 79.5 930.2922 930.0982 4273.9 252 2.747 66.7 1 K.RLKNDKR.W YPL155C 2 2 9.8% 706 78378 9.4 U KIP2 SGDID:S000006076, Chr XVI from 259335-257215, reverse complement, Verified ORF, "Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle" * Jamie_Ashy_SPB_26.09132.09132.4 3.5202 0.0602 19.1 3426.7366 3421.8425 5916.1 5 3.344 19.9 1 H.AIVLIRLTSTNVKNGTSRSSTLSLCDLAGSER.A * Jamie_Ashy_SPB_46.16462.16462.4 2.7045 0.0163 49.6 4307.8965 4304.8164 6269.4 7 2.55 16.7 1 K.NVALHVSKKSIISNGNNDGDKDRTIELLRRQLEEQRR.M Reverse_YFL021W 2 2 9.8% 510 56328 8.6 U GAT1 SGDID:S000001873, Chr VI from 95964-97496, Verified ORF, "Transcriptional activator of genes involved in nitrogen catabolite repression, member of the GATA family of DNA binding proteins; activity and localization regulated by nitrogen limitation and Ure2p" * Jamie_Ashy_SPB_93.33360.33360.2 1.6733 0.0766 12.3 2078.132 2078.333 6205.3 54 3.153 27.5 1 K.KKGAAGPNLSSSPPPTINNNI.K * Jamie_Ashy_SPB_92.33012.33012.4 3.2323 0.1658 20.5 3258.3364 3258.9312 5535.5 1 4.076 25.6 1 S.KIKKLAPKKKRVSSNRRSGPKVISPSPNR.R Reverse_YKR071C 2 2 9.8% 348 38543 5.3 U DRE2 SGDID:S000001779, Chr XI from 575622-574576, reverse complement, Verified ORF, "Protein of unknown function; mutation displays synthetic lethal interaction with the pol3-13 allele of CDC2" * Jamie_Ashy_SPB_55.19803.19803.2 1.8591 0.1489 26.6 2047.6921 2049.251 7186.3 139 3.655 25.0 1 Q.RSHDAKKFSPLNSTSSSAK.K * Jamie_Ashy_SPB_66.23494.23494.2 1.822 0.0581 12.6 1693.4722 1695.867 6801.2 29 3.378 39.3 1 T.EYKANELTISGDNLK.N YMR303C 2 2 9.8% 348 36732 6.7 U ADH2 SGDID:S000004918, Chr XIII from 874336-873290, reverse complement, Verified ORF, "Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1" * Jamie_Ashy_SPB_110.39399.39399.4 3.2341 0.094 16.3 3127.2566 3126.6355 3384.4 345 3.399 21.2 1 K.LEHKDIPVPKPKPNELLINVKYSGVCH.T Jamie_Ashy_SPB_7.02442.02442.1 1.9183 0.0696 96.7 812.35 811.9103 5388.2 19 3.092 75.0 1 R.YVVDTSK.- Reverse_YLR057W 3 3 9.7% 849 96997 5.7 U YLR057W SGDID:S000004047, Chr XII from 255307-257856, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_64.23082.23082.2 1.5554 0.1061 13.2 2494.8123 2491.9573 6861.3 2 3.42 28.6 1 S.LRIFALAMTTLQGSPVTRDPFR.N * Jamie_Ashy_SPB_66.23793.23793.4 3.1493 0.1171 14.7 4770.9766 4776.53 8488.4 3 3.905 16.3 1 L.SPSSYEFLVPYKEKYTLLSSIPLNSGIQHSSGKLLLPFYKNK.E * Jamie_Ashy_SPB_117.41889.41889.2 1.2811 0.0229 61.3 1930.5922 1929.0941 6157.4 4 3.063 32.4 1 K.ERLTAPDSLDVAVNGNEK.D Reverse_YDR333C 3 3 9.7% 723 83426 5.7 U YDR333C SGDID:S000002741, Chr IV from 1135424-1133253, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_75.26899.26899.3 2.5611 0.0076 43.0 3395.1543 3391.7852 9182.5 322 2.835 19.2 1 R.HYDQLISSMEDHLFTVEENRNWLLPFR.A * Jamie_Ashy_SPB_43.15234.15234.2 1.7616 0.1308 15.0 2322.6921 2324.7312 8299.9 62 3.953 26.3 1 C.NLSDFIINAKLARDFVFLAR.Q * Jamie_Ashy_SPB_26.09318.09318.3 2.4982 0.1327 15.6 2657.8743 2656.149 3645.9 155 3.389 26.1 1 N.SRDGQRIFILAVQLLGPVHYPFK.S Reverse_YDR314C 2 2 9.7% 692 82043 9.4 U YDR314C SGDID:S000002722, Chr IV from 1092506-1090428, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_80.28830.28830.2 1.2435 0.2147 17.6 3090.2122 3091.4468 7118.0 13 4.466 16.7 1 K.SLYQYSTYHKSKLIPSSESLEFYRY.C * Jamie_Ashy_SPB_96.34359.34359.4 2.3841 0.2309 19.2 5092.0166 5087.1357 6452.3 353 4.271 14.2 1 R.FWLVLGLLLTRLSDMKFAPDRVDWDKFKKGILKPVSRRLRRN.L YLR427W 3 3 9.7% 670 76317 8.5 U MAG2 SGDID:S000004419, Chr XII from 988424-990436, Uncharacterized ORF, "DNA-3-methyladenine glycosidase II that catalyzes of the hydrolysis of alkylated DNA" * Jamie_Ashy_SPB_98.35288.35288.3 2.5291 0.024 47.1 2969.3943 2971.1423 7042.0 15 3.343 20.7 1 K.KASGGSGGSEMDTLNATSNSSKQGVSNNKR.N * Jamie_Ashy_SPB_37.13362.13362.2 1.7536 0.1212 17.8 1623.2322 1620.7119 9536.6 172 3.433 37.5 1 K.YDDSSAYFFYQTL.V * Jamie_Ashy_SPB_91.32510.32510.2 1.2481 0.0026 60.3 2613.612 2616.0781 10020.6 11 3.232 26.2 1 R.YKYIGHLPIGTEIALLDLDWRK.I YDL113C 2 2 9.7% 640 72546 7.0 U ATG20 SGDID:S000002271, Chr IV from 258555-256633, reverse complement, Verified ORF, "Protein required for transport of aminopeptidase I (Lap4p) through the cytoplasm-to-vacuole targeting pathway; binds phosphatidylinositol-3-phosphate, involved in localization of membranes to the preautophagosome, potential Cdc28p substrate" * Jamie_Ashy_SPB_81.29151.29151.3 2.7023 0.1441 16.3 3189.8643 3186.731 6317.6 127 3.705 18.5 1 R.KKSARIHILEAKRVSEGQGRAYIAYVIQ.F * Jamie_Ashy_SPB_37.13201.13201.4 2.8732 0.1026 15.6 4049.2966 4049.7703 6622.3 379 2.832 15.7 1 E.KLVGRLYYNINEPLNESVHMATSARELIKYRKLK.Y Reverse_YLR417W 2 2 9.7% 566 64018 6.4 U VPS36 SGDID:S000004409, Chr XII from 955006-956706, Verified ORF, "Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome" * Jamie_Ashy_SPB_101.36266.36266.2 1.6457 0.1485 16.0 2550.632 2551.9866 7938.1 44 4.808 27.3 1 S.LIQTLRLLDSGPNDGILDVLKEK.V * Jamie_Ashy_SPB_50.18000.18000.4 3.2416 0.0987 17.1 3817.4565 3815.3362 7679.2 12 3.191 18.8 1 S.HSSKNRALSNKELEIHVRSDHFNLRNLKSRVK.S YML023C 2 2 9.7% 556 64013 6.0 U YML023C SGDID:S000004485, Chr XIII from 228664-226994, reverse complement, Uncharacterized ORF, "Protein required for cell viability" * Jamie_Ashy_SPB_19.06807.06807.2 2.0631 0.0341 15.6 1318.1522 1317.5344 5582.3 241 2.7 55.0 1 T.SRETLSRRALK.L * Jamie_Ashy_SPB_28.10083.10083.4 2.3219 0.0341 43.1 4865.9365 4866.491 5195.9 387 3.155 13.5 1 K.LPSDNYATPVHPVYNGENTIVDTYIPTIKCSPLYKSQKSLALR.R YGR211W 2 2 9.7% 486 55072 4.9 U ZPR1 SGDID:S000003443, Chr VII from 915246-916706, Verified ORF, "Essential protein with two zinc fingers, present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p)" * Jamie_Ashy_SPB_63.22479.22479.2 1.6549 0.1205 14.9 1684.7922 1682.8486 5649.5 1 3.501 37.5 1 K.ANDGVKLTGAQDAMGHP.V * Jamie_Ashy_SPB_17.05919.05919.4 3.0724 0.0783 11.7 3390.2566 3390.0205 6076.2 421 3.089 20.1 1 R.WVEFFAKLKEAIAGKVKFTVIMEDPLAGSY.I Reverse_YDR034C 3 3 9.6% 790 89397 6.9 U LYS14 SGDID:S000002441, Chr IV from 512105-509733, reverse complement, Verified ORF, "Transcriptional activator involved in regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer" * Jamie_Ashy_SPB_34.12265.12265.2 1.7901 0.0481 13.3 1751.3121 1750.9059 6236.7 403 2.954 38.5 1 W.TLVDQEELRYNQNK.G * Jamie_Ashy_SPB_31.10986.10986.3 2.7101 0.15 10.9 2065.5544 2066.2756 8087.2 124 3.756 31.9 1 R.EDELNNSKRYAISAGTALI.S * Jamie_Ashy_SPB_72.25824.25824.4 2.5331 0.1497 17.9 4602.6567 4597.913 7937.0 30 3.988 14.7 1 R.SYKRKVTNGKKDTVTSTVAPNPTLSSHGNDDAQSITPHDANQD.Q Reverse_YBR102C 3 3 9.6% 753 85538 7.9 U EXO84 SGDID:S000000306, Chr II from 447317-445056, reverse complement, Verified ORF, "Essential protein with dual roles in spliceosome assembly and exocytosis; the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p) mediates polarized targeting of secretory vesicles to active sites of exocytosis" * Jamie_Ashy_SPB_10.03600.03600.2 1.4315 0.1945 19.5 1509.4521 1507.5629 5263.3 10 3.979 45.5 1 R.KSNEEEVHFIDC.L * Jamie_Ashy_SPB_55.19813.19813.4 3.7433 0.0111 76.5 3777.8564 3778.2078 6681.4 12 4.042 18.2 1 K.VEEQVQISLDTLNSTFKDITIASANGLKDHVFDK.A * Jamie_Ashy_SPB_104.37465.37465.3 2.12 0.0023 34.1 2783.4543 2786.2937 5881.1 56 3.157 21.0 1 K.TKQKPKAPSPPTKQKAPSSASKKVHK.W YIL022W 2 2 9.5% 431 48854 9.4 U TIM44 SGDID:S000001284, Chr IX from 311163-312458, Verified ORF, "Peripheral mitochondrial membrane protein involved in mitochondrial protein import, tethers essential chaperone Ssc1p to the translocon channel at the matrix side of the inner membrane" * Jamie_Ashy_SPB_49.17605.17605.4 2.7255 0.1827 10.1 3588.6565 3594.0684 10750.0 286 3.713 18.3 1 G.TSSRTLTARYRSQYTGLLVARVLFSTSTTRAQ.G * Jamie_Ashy_SPB_25.08974.08974.2 1.2747 6.0E-4 60.9 860.09216 860.04456 2744.1 24 2.517 62.5 1 R.KAAAATAKK.L Reverse_YKR063C 2 2 9.4% 502 59352 7.8 U LAS1 SGDID:S000001771, Chr XI from 561939-560431, reverse complement, Verified ORF, "Essential nuclear protein possibly involved in bud formation and morphogenesis; mutants require the SSD1-v allele for viability" * Jamie_Ashy_SPB_17.05910.05910.4 2.636 0.1325 18.2 3641.5764 3636.166 3811.4 10 3.365 19.6 1 T.ELKALMSQCFYLILYSANEDILKEWNQWK.N * Jamie_Ashy_SPB_76.27225.27225.2 2.0355 0.1309 28.6 2013.1522 2013.4058 7014.3 25 3.755 35.3 1 R.ILAMVYSLRIPISDQHVG.L YMR068W 2 2 9.4% 426 47140 9.3 U AVO2 SGDID:S000004672, Chr XIII from 406303-407583, Verified ORF, "Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth" * Jamie_Ashy_SPB_117.42144.42144.2 1.3343 0.1488 12.8 2603.0122 2605.0613 9009.0 301 3.6 25.0 1 R.YLICVYLIQLGHDKHELIKTF.K * Jamie_Ashy_SPB_7.02551.02551.3 2.506 0.1989 18.9 2264.8442 2261.6934 5526.9 287 4.117 27.8 1 L.ALMKGHEQTLHLLLQQFPR.F YJR080C 2 2 9.4% 394 44428 9.8 U YJR080C SGDID:S000003841, Chr X from 581530-580346, reverse complement, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * Jamie_Ashy_SPB_51.18387.18387.2 1.8446 0.139 22.2 2265.0923 2267.639 9452.2 28 3.879 30.6 1 F.IKNQTLKSAYGHTVQFFKR.M * Jamie_Ashy_SPB_7.02394.02394.4 2.7625 0.1358 13.5 2064.8167 2060.3704 6013.1 422 3.513 30.4 1 R.TILIQTHEMTTSKDNILT.K YDL028C 3 3 9.3% 764 86828 8.9 U MPS1 SGDID:S000002186, Chr IV from 403288-400994, reverse complement, Verified ORF, "Dual-specificity kinase required for spindle pole body (SPB) duplication and spindle checkpoint function; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, and checkpoint protein Mad1p" * Jamie_Ashy_SPB_72.25708.25708.4 1.9459 0.1499 69.1 6424.9365 6421.182 9466.4 384 3.423 10.7 1 R.YLRNNHQTSMTTMNTSDIEINVGNSLDKSFERIRNLRQNMKEDITAKYAERRSK.R * Jamie_Ashy_SPB_22.07939.07939.3 2.7135 0.101 18.7 1924.8544 1925.1669 5785.6 57 3.585 31.7 1 R.NLRQNMKEDITAKYAE.R * Jamie_Ashy_SPB_41.14731.14731.2 1.6013 0.0202 63.2 2065.2522 2066.3923 7483.2 306 3.478 31.2 1 K.SAIELMKACLYRNPDKR.W Reverse_YBR156C 2 2 9.3% 698 79186 9.9 U SLI15 SGDID:S000000360, Chr II from 553194-551098, reverse complement, Verified ORF, "Subunit of the Ipl1p-Sli15p-Bir1p complex that regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; regulates the activity and localization of the Ipl1p aurora kinase" * Jamie_Ashy_SPB_80.28578.28578.4 2.538 0.0434 53.0 4254.2563 4252.6475 8040.6 75 2.945 14.5 1 K.HGVNGRSIKNEFSKTPISSLRDFVSSAKTSGATDSNEVAR.I * Jamie_Ashy_SPB_21.07413.07413.4 3.0158 0.0879 15.1 2894.6165 2894.211 7045.5 3 2.956 25.0 1 S.SERLVKEVKYPSWPSSKESENADVK.V Reverse_YER147C 2 2 9.3% 624 72141 7.8 U SCC4 SGDID:S000000949, Chr V from 464837-462963, reverse complement, Verified ORF, "Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX" * Jamie_Ashy_SPB_17.05859.05859.4 2.7281 0.136 14.7 2127.8965 2130.5107 4486.8 58 3.755 28.9 1 Q.IRSDLENMSVGGAAINKILK.Q * Jamie_Ashy_SPB_25.09028.09028.4 2.3417 0.0231 42.0 4463.8965 4469.1646 6671.4 240 2.874 14.4 1 K.CLQIVMELALKWAYLEPGVTHLETSRLEQLADEPIPFR.E YPL070W 2 2 9.3% 612 69539 8.5 U MUK1 SGDID:S000005991, Chr XVI from 420945-422783, Uncharacterized ORF, "Protein of unknown function, localized to the cytoplasm; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation" * Jamie_Ashy_SPB_23.08259.08259.2 2.1545 0.0722 17.8 1780.5322 1779.7716 6350.4 315 3.145 36.7 1 C.DGDENSTTGERLENNK.S * Jamie_Ashy_SPB_102.36590.36590.4 1.9283 0.0794 43.1 4712.177 4710.466 7666.4 141 3.232 14.2 1 K.SPILTKQEIDEALNTVTNLPPELSKLIDIFIDDLKQPKYVR.P Reverse_YLR353W 2 2 9.3% 603 66289 6.3 U BUD8 SGDID:S000004345, Chr XII from 834351-836162, Verified ORF, "Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole" * Jamie_Ashy_SPB_102.36428.36428.4 2.9903 0.0987 17.0 3181.5366 3177.4924 5285.7 158 3.555 20.7 1 K.EIISNQGTDSESSQSKIPALVLPQGHEPLT.K * Jamie_Ashy_SPB_94.33630.33630.3 2.2841 0.0495 47.0 2540.9644 2543.7532 7408.8 6 3.61 25.0 1 K.IKRASTGPAGPSHSDDSTSSKISGAK.E Reverse_YDR388W 2 2 9.3% 482 52774 6.0 U RVS167 SGDID:S000002796, Chr IV from 1250176-1251624, Verified ORF, "Actin-associated protein, subunit of a complex (Rvs161p-Rvs167p) involved in regulation of actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; homolog of mammalian amphiphysin" * Jamie_Ashy_SPB_100.36045.36045.2 1.6589 0.0663 10.6 1940.7322 1938.1045 7341.3 121 3.282 30.0 1 Y.GVGSSAGSVPSGEATAVGYKR.R * Jamie_Ashy_SPB_31.11004.11004.4 3.5629 0.1381 19.7 2780.3765 2781.2256 5698.9 288 3.491 20.3 1 K.LLEQCPTVIKEEVLALDPKLTEQL.E YPL049C 2 2 9.3% 452 49356 8.6 U DIG1 SGDID:S000005970, Chr XVI from 463836-462478, reverse complement, Verified ORF, "Regulatory protein of unknown function, constitutively-expressed, involved in the regulation of mating-specific genes and the invasive growth pathway, required for MAP-kinase imposed repression, inhibits pheromone-responsive transcription" * Jamie_Ashy_SPB_59.21258.21258.2 1.8112 0.0821 85.8 2112.5923 2113.2485 7640.4 16 3.505 28.9 1 R.VNDSYDSPLSGTASTGKTRR.S * Jamie_Ashy_SPB_111.39703.39703.2 1.3799 0.1431 13.5 2438.612 2435.6995 6510.4 259 3.476 16.7 1 R.TSQPQQQSPSLLQGEIRLSSHI.F YNR074C 3 3 9.3% 378 41335 7.4 U AIF1 SGDID:S000005357, Chr XIV from 778738-777602, reverse complement, Verified ORF, "Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase" * Jamie_Ashy_SPB_4.01471.01471.2 1.6417 0.0654 13.1 1468.4321 1468.5652 3847.9 4 3.154 50.0 1 T.FGDNYKEYFER.E * Jamie_Ashy_SPB_39.13944.13944.2 1.933 0.0159 13.4 1453.9122 1453.7216 7156.6 199 3.076 45.0 1 G.ELLFKYLEEIR.S * Jamie_Ashy_SPB_35.12464.12464.2 1.7481 0.0536 12.8 1344.3322 1346.4832 5860.5 378 3.1 50.0 1 L.NTVGASLDTSPKR.I YPL137C 5 5 9.2% 1276 140891 7.4 U YPL137C SGDID:S000006058, Chr XVI from 296646-292816, reverse complement, Uncharacterized ORF, "Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus" * Jamie_Ashy_SPB_28.09879.09879.4 3.2494 0.1465 10.9 2925.2166 2928.954 6327.2 76 3.662 21.8 1 T.NTKPSRPSTSPASSSSTSSSKNGDNSTSGN.R * Jamie_Ashy_SPB_16.05647.05647.2 1.7604 0.0094 73.4 1032.3522 1031.1166 5502.5 31 2.986 68.8 1 R.SSNDKPRAR.S * Jamie_Ashy_SPB_61.21648.21648.4 2.8829 0.0739 76.2 3886.5366 3891.5872 7533.5 113 2.964 16.7 1 R.GVAPTASKPQTPILPSPALAVKDLSTVSLKRVSFAVDK.F * Jamie_Ashy_SPB_32.11460.11460.2 2.1407 0.0558 15.6 2036.5521 2037.4865 6020.9 169 2.892 32.4 1 T.MIKPMVGKLSALSYDNLR.Y * Jamie_Ashy_SPB_108.38726.38726.2 1.5609 0.078 10.1 2359.0723 2360.734 8816.7 108 3.413 23.8 1 V.ITARPHLMATDSGKTIDVFTGK.P YLR383W 3 3 9.2% 1114 128008 7.5 U SMC6 SGDID:S000004375, Chr XII from 885288-888632, Verified ORF, "Protein involved in structural maintenance of chromosomes; required for interchromosomal and sister chromatid recombination; homologous to S. pombe rad18" * Jamie_Ashy_SPB_43.15243.15243.4 3.7265 0.1273 21.1 4261.3765 4266.8037 5549.6 18 3.642 17.1 1 G.AYQQGIFGNEIIVERIIKRDGPASFSLRSENGKEISNK.K * Jamie_Ashy_SPB_85.30607.30607.2 1.5308 0.1523 15.7 2455.9321 2458.7336 8419.4 68 3.369 22.5 1 T.YQDKIRLKVNSSSDNGTQYLK.D * Jamie_Ashy_SPB_44.15765.15765.4 3.0412 0.1987 39.6 4984.9365 4986.909 6856.7 2 4.596 15.9 1 R.IIALDEFDVFMDQVNRKIGTTLIVKKLKDIARTQTIIITPQDIG.K Reverse_YAL005C 2 2 9.2% 642 69767 5.1 U SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport; member of heat shock protein 70 (HSP70) family; forms a chaperone complex with Ydj1p; localized to the nucleus, cytoplasm, and cell wall" * Jamie_Ashy_SPB_60.21523.21523.4 3.1487 0.0758 14.5 4363.0166 4361.0127 5466.0 83 3.03 14.9 1 R.EGEFVQILVGPQNDAYTSFIEFKKTSITSNRPILKTMVG.G Jamie_Ashy_SPB_56.20014.20014.2 1.9596 0.1137 21.0 1924.7922 1924.0715 6630.3 32 3.606 28.9 1 K.SSEDGTLIAAQVAAGYAVAE.D YLR071C 3 3 9.1% 1082 123358 8.7 U RGR1 SGDID:S000004061, Chr XII from 278627-275379, reverse complement, Verified ORF, "Component of RNA polymerase II holoenzyme/mediator complex; affects chromatin structure and transcriptional regulation of diverse genes; required for glucose repression, HO repression, RME1 repression and sporulation" * Jamie_Ashy_SPB_101.36396.36396.2 1.0708 0.0806 62.0 3164.7922 3164.78 5433.6 101 3.583 13.0 1 K.DLNLTVSIKIALMNIPKPLNSYHIKNGR.I * Jamie_Ashy_SPB_75.26718.26718.4 3.0132 0.0999 82.9 4439.7764 4442.575 7745.7 455 3.183 15.0 1 K.LLFNTEAEQTVSAVTEATSTNGDSENNEENSSSNGNNLPLNK.P * Jamie_Ashy_SPB_95.33903.33903.4 2.9175 0.127 16.3 2963.5366 2962.2554 6378.1 32 3.257 24.4 1 R.LGTPESVKPANASHAIRLGNGVACDPSEI.E YBR001C 2 2 9.1% 780 89679 6.5 U NTH2 SGDID:S000000205, Chr II from 241285-238943, reverse complement, Verified ORF, "Putative neutral trehalase, required for thermotolerance and may mediate resistance to other cellular stresses" * Jamie_Ashy_SPB_85.30342.30342.4 2.8039 0.11 15.4 4789.2964 4794.3438 8686.2 24 3.282 15.4 1 R.RVDLYNIAEIARDSKIDTPGAKNPRIYVPYNCPEQYEFYI.Q * Jamie_Ashy_SPB_30.10687.10687.4 3.0615 0.0936 17.9 3602.3364 3605.146 5714.4 117 3.234 17.8 1 M.NNHARRALAACSPPLPFFNSLKPSEKKLYYL.- Reverse_YIR003W 3 3 9.1% 679 74763 5.0 U YIR003W SGDID:S000001442, Chr IX from 360882-362921, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_43.15500.15500.4 3.2886 0.1248 10.2 3143.0566 3140.504 4796.2 100 4.37 21.8 1 S.NGNNHLDQQQQKQLMEQFAAIRSSPKK.P * Jamie_Ashy_SPB_12.04114.04114.1 1.6904 0.0904 90.9 1216.4 1216.3336 5030.6 8 3.69 55.6 1 R.KEQTNPLEEK.Q * Jamie_Ashy_SPB_75.26844.26844.4 2.3735 0.0342 44.9 2777.2166 2781.0723 4580.9 124 2.619 23.6 1 K.MDGPILKEEFHSRDGAENDLAAHVK.Q Reverse_YDR200C 2 2 9.1% 604 67284 7.4 U VPS64 SGDID:S000002608, Chr IV from 856088-854274, reverse complement, Verified ORF, "Cytoplasmic protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p, Far7p, Far10p, and Far11p that is involved in pheromone-induced cell cycle arrest; also localized to the endoplasmic reticulum membrane" * Jamie_Ashy_SPB_47.16831.16831.3 2.6334 0.0454 58.2 2711.9343 2711.8674 10764.1 9 3.423 23.9 1 R.SDFNGNDPRVQQSFLDRKSGSNNK.N * Jamie_Ashy_SPB_81.29059.29059.4 2.694 0.1728 90.0 3592.8564 3593.3396 6291.2 5 3.628 20.0 1 K.LGDPKFPVVLFKTEFTANLSKLIIIHTHKKR.P YIL146C 3 3 9.1% 529 58969 7.0 U ECM37 SGDID:S000001408, Chr IX from 75773-74184, reverse complement, Verified ORF, "Non-essential protein of unknown function" * Jamie_Ashy_SPB_103.36854.36854.2 1.6163 0.0414 68.9 1682.7922 1682.8333 7409.4 150 3.015 40.0 1 K.QGQEGANVVSPSHPFK.Q * Jamie_Ashy_SPB_65.23286.23286.4 3.1548 0.0922 17.7 3845.1765 3840.035 6362.0 280 3.301 18.3 1 G.EDVNEEDNDDEAIHTKSRSYCRNKKAENSKKK.S * Jamie_Ashy_SPB_8.02745.02745.2 2.0072 0.0307 16.1 941.65216 942.06287 5144.4 4 3.868 78.6 1 D.EAIHTKSR.S YBL055C 2 2 9.1% 418 47390 6.7 U YBL055C SGDID:S000000151, Chr II from 116832-115576, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_12.04039.04039.2 2.084 0.1122 29.2 1584.0322 1583.7434 6546.9 12 4.004 50.0 1 I.YNGKQYHPADYVK.L * Jamie_Ashy_SPB_7.02208.02208.4 3.1716 0.1245 13.0 3027.2166 3028.3784 5315.8 101 4.13 22.9 1 F.LHMRSACDDFVQILERFIAGFTDER.D YCR019W 2 2 9.1% 363 40783 6.3 U MAK32 SGDID:S000000612, Chr III from 152834-153925, Verified ORF, "Protein necessary for structural stability of L-A double-stranded RNA-containing particles" * Jamie_Ashy_SPB_93.33271.33271.4 2.6805 0.1195 15.6 3155.2166 3153.4814 6376.4 253 3.393 21.3 1 D.VRFRDDFSRLTTKGLNYYEGSDDLRK.F * Jamie_Ashy_SPB_26.09228.09228.4 3.4244 0.0488 11.6 3076.3364 3079.526 5082.4 191 2.791 20.7 1 F.SRLTTKGLNYYEGSDDLRKFKFLTPK.K Reverse_YDR495C 3 3 9.0% 1011 116922 5.1 U VPS3 SGDID:S000002903, Chr IV from 1441140-1438105, reverse complement, Verified ORF, "Cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase" * Jamie_Ashy_SPB_82.29298.29298.2 1.7581 0.1181 15.2 2193.9521 2195.5176 7805.4 22 3.326 29.4 1 R.EKIYDEPLKKLNEEIFSV.L * Jamie_Ashy_SPB_27.09626.09626.3 1.6554 0.0969 39.3 4112.514 4115.247 5291.9 112 3.524 11.1 1 K.ENLSTIDTNIVNDEASSPQCSEQATNKDTHPSDEIPR.S * Jamie_Ashy_SPB_49.17336.17336.4 2.9595 0.1065 18.3 4785.3765 4779.9136 8642.3 6 3.384 15.0 1 P.SDEIPRSEEQSFKDARVTENPNANHTNTEESETARLSDDETK.K Reverse_YJR036C 2 2 9.0% 892 103456 8.8 U HUL4 SGDID:S000003797, Chr X from 503305-500627, reverse complement, Verified ORF, "Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability" * Jamie_Ashy_SPB_70.24942.24942.4 3.0803 0.0897 17.0 4071.2166 4065.7014 9073.4 473 3.337 16.2 1 K.FPITSIGTAPIRDSATVFQLLKKQLPYDWSEIIEW.F * Jamie_Ashy_SPB_100.36036.36036.4 2.4045 0.2067 11.5 4847.0967 4849.148 8595.1 13 4.259 13.6 1 R.LPVGCCLCNLSILCSKDEEGSVSASSNRKNSKSNSCSGDKVSAAL.S Reverse_YLR335W 2 2 9.0% 720 77881 7.2 U NUP2 SGDID:S000004327, Chr XII from 797430-799592, Verified ORF, "Protein involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization" * Jamie_Ashy_SPB_34.12092.12092.4 3.4794 0.1033 11.0 4086.8965 4092.3784 7457.4 319 3.624 15.4 1 N.NDDEQSAEKASPTAAGFSFVPKSTENKSSNPVGFSFSPK.S * Jamie_Ashy_SPB_17.05931.05931.4 3.3185 0.1243 91.1 2863.3765 2865.0369 5537.4 62 3.42 22.7 1 K.EPKSNSTSNEDNKKEITTSGFTFSSK.E YBR170C 2 2 9.0% 580 65782 5.7 U NPL4 SGDID:S000000374, Chr II from 578081-576339, reverse complement, Verified ORF, "Endoplasmic reticulum and nuclear membrane protein, forms a complex with Cdc48p and Ufd1p that recognizes ubiquitinated proteins in the endoplasmic reticulum and delivers them to the proteasome for degradation" * Jamie_Ashy_SPB_48.17097.17097.3 2.8823 0.1257 15.6 3268.9744 3270.503 8196.2 60 3.289 19.8 1 G.NLDPNADVDTFTVCEKPGQGIHAVSELADR.T * Jamie_Ashy_SPB_47.16882.16882.2 1.7781 0.0931 16.7 2406.5723 2407.553 8047.7 305 3.478 23.8 1 K.LNENANKKENGSSYISPLSEPD.F Reverse_YGR276C 2 2 9.0% 553 62849 7.2 U RNH70 SGDID:S000003508, Chr VII from 1045486-1043825, reverse complement, Verified ORF, "3' exoribonuclease, required for 5S and tRNA-Arg3 maturation" * Jamie_Ashy_SPB_84.29996.29996.4 3.4102 0.0523 11.9 3975.5366 3974.7405 7478.5 9 3.23 20.1 1 I.IMDNLLVTKVEFRHLKTFLNETNIGIGFALGNLIK.L * Jamie_Ashy_SPB_63.22494.22494.2 1.8046 0.0597 11.8 1628.7322 1625.8192 7116.0 159 3.35 39.3 1 K.AGVTLKEETIGSYRT.L Reverse_YPL262W 2 2 9.0% 488 53152 8.2 U FUM1 SGDID:S000006183, Chr XVI from 47336-48802, Verified ORF, "Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial localization determined by the N-terminal mitochondrial targeting sequence and protein conformation" * Jamie_Ashy_SPB_11.03848.03848.3 3.0312 0.0839 13.2 3291.2043 3291.5308 4375.2 126 3.553 19.0 1 F.TDNSSQSQNCHNNPHVQKSGIKGGLIEIAR.N * Jamie_Ashy_SPB_102.36728.36728.2 1.5371 0.1439 15.7 1622.3722 1622.9719 6712.0 1 3.723 53.8 1 L.VGFAHVLPLPMRER.A YLR146C 2 2 9.0% 300 34091 5.1 U SPE4 SGDID:S000004136, Chr XII from 433726-432824, reverse complement, Verified ORF, "Spermine synthase, required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid" * Jamie_Ashy_SPB_3.00874.00874.2 1.6075 0.0779 77.7 1583.5721 1582.71 5490.5 54 3.61 38.5 1 K.FLPTLSNGAFDDER.L * Jamie_Ashy_SPB_30.10662.10662.2 1.8156 0.1144 15.7 1572.2122 1572.805 8978.2 8 3.923 50.0 1 P.QRKISEQEQGKLK.Y Reverse_YLR451W 2 2 8.9% 886 100153 6.1 U LEU3 SGDID:S000004443, Chr XII from 1036089-1038749, Verified ORF, "Zinc-finger transcription factor that regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; positively regulated by alpha-isopropylmalate, an intermediate in leucine biosynthesis" * Jamie_Ashy_SPB_27.09639.09639.4 2.5565 0.1732 12.6 5402.1367 5406.2026 7830.3 6 4.203 14.2 1 R.YAMDYKLVSANFTLSIANNYAKKGSNVDLMSHLSSHILKSIICASQWI.N * Jamie_Ashy_SPB_96.34452.34452.4 3.0561 0.0977 18.9 3417.2966 3419.8 4834.4 2 3.665 22.2 1 R.IQENVLESNASAYEKSFGACNLGVRLAQFMA.T YLR176C 3 3 8.9% 811 90584 8.8 U RFX1 SGDID:S000004166, Chr XII from 510234-507799, reverse complement, Verified ORF, "Protein involved in DNA damage and replication checkpoint pathway; recruits repressors Tup1p and Cyc8p to promoters of DNA damage-inducible genes; similar to a family of mammalian DNA binding RFX1-4 proteins" * Jamie_Ashy_SPB_53.18805.18805.3 2.4203 0.185 11.7 2177.9644 2181.416 5160.9 28 4.017 28.8 1 G.PGSHKPSPTPTQPPAQPATQR.Q * Jamie_Ashy_SPB_74.26557.26557.4 3.382 0.0551 12.7 4568.4165 4572.0454 9034.9 171 4.296 14.2 1 R.SNSSAYPLSFKSEETLNNNPPTAAKRTNTFPSIPSSTKKQKT.S * Jamie_Ashy_SPB_22.07872.07872.2 1.2935 0.0178 61.9 1091.6322 1089.1527 4842.8 91 2.781 56.2 1 K.SQHDSYVPR.G YMR192W 3 3 8.9% 720 82131 5.4 U GYL1 SGDID:S000004804, Chr XIII from 647117-649279, Verified ORF, "Putative GTPase activating protein (GAP) that may have a role in polarized exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p" * Jamie_Ashy_SPB_40.14278.14278.4 2.7243 0.203 93.8 4992.9365 4992.3906 5448.9 157 4.432 13.6 1 K.DGESIGAFSNPHEALMQSKLREESQSKTILPSDDLSQQLETEESK.V * Jamie_Ashy_SPB_16.05550.05550.2 1.6918 0.0765 15.9 865.7922 863.90283 3792.1 136 3.465 66.7 1 L.REESQSK.T * Jamie_Ashy_SPB_12.04208.04208.3 2.9305 0.0177 66.0 2296.3442 2295.6284 5710.8 1 2.805 36.1 1 K.KDLEETMKKNTQVMDENLK.L Reverse_YDR026C 2 2 8.9% 570 66352 9.3 U YDR026C SGDID:S000002433, Chr IV from 494264-492552, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_101.36241.36241.4 2.4409 0.0726 61.9 4471.4966 4475.0386 8333.6 339 2.977 16.7 1 K.ELCLRALEQDEEPTWKGRQEFIHYKRRIHKYISSR.T * Jamie_Ashy_SPB_39.13971.13971.4 2.9222 0.0527 73.4 2913.9365 2909.3665 5555.7 449 3.055 22.5 1 R.IHKYISSRTRYPLVKSINAWFGDR.I Reverse_YML093W 4 4 8.8% 899 103023 5.1 U UTP14 SGDID:S000004558, Chr XIII from 83090-85789, Verified ORF, "Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA" * Jamie_Ashy_SPB_81.29018.29018.4 3.3411 0.1004 14.2 3045.8164 3042.6443 4385.4 9 3.835 23.1 1 K.DKNVVGKVKKIFKRKKNKPKSGAGAWE.G * Jamie_Ashy_SPB_103.36894.36894.3 2.5205 0.0815 71.3 2330.2744 2329.5742 5612.1 168 3.917 26.2 1 K.NKPKSGAGAWEGWGPLTTDVEK.D * Jamie_Ashy_SPB_57.20366.20366.3 2.5223 0.0491 54.6 3417.7444 3421.6157 8585.4 161 3.063 17.2 1 R.RGKNIQINEGNTEEEDSEFLKIDDGNEVAR.L * Jamie_Ashy_SPB_23.07982.07982.1 1.2311 0.1481 89.4 1280.47 1279.4855 6838.6 134 3.42 40.0 1 R.RSSKSRSKSKK.K Reverse_YOR353C 2 2 8.8% 791 87326 7.4 U SOG2 SGDID:S000005880, Chr XV from 1000823-998448, reverse complement, Verified ORF, "Protein required for cell morphogenesis and cell separation after mitosis" * Jamie_Ashy_SPB_51.18213.18213.3 3.2231 0.1678 21.7 2877.6543 2876.9768 7754.2 32 3.681 24.1 1 L.KINGSCHTSTTSAAPGGGSTISSSSTEHR.S * Jamie_Ashy_SPB_116.41691.41691.4 1.1914 6.0E-4 32.6 4757.0967 4761.383 4181.9 319 3.17 7.9 1 R.SIAIAWYEEKPIGTHFPTYSQVQDLEEKPIKNDELDLISLK.N YKR024C 3 3 8.8% 742 83308 9.3 U DBP7 SGDID:S000001732, Chr XI from 487015-484787, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis" * Jamie_Ashy_SPB_79.28342.28342.4 2.476 0.0173 43.3 3296.1765 3292.7695 7326.2 75 3.039 20.1 1 K.TVSRKRKANTTGDEGIIPGRGENSIKKLHK.E * Jamie_Ashy_SPB_43.15471.15471.4 2.659 0.1482 13.4 2312.8965 2310.4363 4329.3 2 4.058 30.7 1 R.GENSIKKLHKESSYSSEEQE.K * Jamie_Ashy_SPB_41.14646.14646.4 2.8476 0.1567 12.6 2807.6165 2802.3306 8193.9 3 3.952 24.3 1 K.DSDIVTVAPDQLLQRITIVPPKLRL.V Reverse_YLR023C 3 3 8.8% 543 62571 9.2 U IZH3 SGDID:S000004013, Chr XII from 187128-185497, reverse complement, Verified ORF, "Membrane protein involved in zinc metabolism, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity" * Jamie_Ashy_SPB_15.05110.05110.2 1.9015 0.1071 18.1 1382.1522 1382.5101 5021.4 6 3.417 59.1 1 L.DTSLEYNTPITK.D * Jamie_Ashy_SPB_86.30708.30708.3 2.6606 0.1343 18.4 3320.8442 3316.752 7602.2 18 3.408 23.0 1 Y.WNFISLLSKSHTNYFRYGHIIYRNER.W * Jamie_Ashy_SPB_17.06078.06078.2 1.5122 0.1269 15.3 1086.0122 1087.2483 6878.8 122 3.717 55.6 1 Q.TLSKSSSDMM.- YBR083W 2 2 8.8% 486 55158 7.6 U TEC1 SGDID:S000000287, Chr II from 409163-410623, Verified ORF, "Transcription factor required for full Ty1 epxression, Ty1-mediated gene activation, and haploid invasive and diploid pseudohyphal growth; TEA/ATTS DNA-binding domain family member" * Jamie_Ashy_SPB_73.25970.25970.2 1.459 0.1601 16.0 2867.2322 2867.1472 9509.3 5 4.118 23.9 1 D.DFGKDNSRNIESYTGRIFDVYIQK.D * Jamie_Ashy_SPB_13.04375.04375.2 1.676 0.1526 10.7 2131.8123 2133.4587 6756.6 2 3.699 33.3 1 N.GTTKIKIRNANFGRNELIS.L Reverse_YJR062C 3 3 8.8% 457 51897 5.2 U NTA1 SGDID:S000003823, Chr X from 554763-553390, reverse complement, Verified ORF, "Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation" * Jamie_Ashy_SPB_12.04256.04256.2 1.9495 0.0073 11.8 1246.9722 1247.3042 5218.6 216 2.636 60.0 1 S.EDDDQSGKPKK.G * Jamie_Ashy_SPB_84.30267.30267.2 1.5879 0.0955 12.2 1734.6322 1736.7957 7586.3 124 3.903 31.2 1 K.YIGSSGAFITTGDETGC.R * Jamie_Ashy_SPB_45.15991.15991.2 1.9621 0.1548 35.8 1439.8722 1440.5095 6114.3 1 3.679 54.5 1 T.VDRNYSDENSLK.A YBR166C 3 3 8.8% 452 50923 6.6 U TYR1 SGDID:S000000370, Chr II from 571195-569837, reverse complement, Verified ORF, "Prephenate dehydrogenase involved in tyrosine biosynthesis, expression is dependent on phenylalanine levels" * Jamie_Ashy_SPB_13.04500.04500.2 1.6897 0.0837 14.0 1397.9321 1397.5272 5499.6 138 3.651 45.0 1 K.SKQVYLTYEEH.D * Jamie_Ashy_SPB_14.04910.04910.2 1.4265 0.1688 17.6 952.9122 952.9195 5006.0 136 4.219 56.2 1 K.SSIGNSNNC.K * Jamie_Ashy_SPB_33.11601.11601.2 1.4425 0.1913 10.4 2409.4722 2407.8826 7402.1 33 4.153 26.3 1 H.MICSTPLFRIFLGVSEYLFL.K YJR127C 3 3 8.7% 1380 155062 8.0 U RSF2 SGDID:S000003888, Chr X from 662974-658832, reverse complement, Verified ORF, "Zinc-finger protein involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions" * Jamie_Ashy_SPB_104.37222.37222.4 2.8149 0.1347 17.0 3981.4966 3982.5217 6659.0 264 3.938 18.8 1 R.IIKTDKPRPFLCPTCTRGFVRQEHLKRHQHSH.T * Jamie_Ashy_SPB_60.21571.21571.4 3.1969 0.151 10.8 4620.4966 4619.048 6573.8 57 3.69 13.8 1 S.YSNLANQTYRQMSNEQPLMSLSPKNPPTTVSDSSSTINFNPG.T * Jamie_Ashy_SPB_58.20778.20778.4 3.1649 0.1785 10.3 5220.9766 5224.7764 8125.1 2 3.827 15.2 1 P.FSVPSVSQVLFTNELRSMMLADNNIDSGAFPTTSQLNDYVTYYKEE.F YHR120W 3 3 8.7% 959 109408 8.7 U MSH1 SGDID:S000001162, Chr VIII from 349577-352456, Verified ORF, "DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis" * Jamie_Ashy_SPB_55.19488.19488.2 1.5308 0.1271 15.8 1406.2522 1406.6249 5929.2 6 3.301 54.5 1 F.QTFTQKELAALR.N * Jamie_Ashy_SPB_56.20134.20134.4 3.027 0.1324 89.6 4707.2163 4706.3555 7037.0 404 3.818 13.9 1 K.TEYNALLKGFFVGDLGAKTFTLKERQNGEYALHVTGTASSLKK.I * Jamie_Ashy_SPB_35.12608.12608.3 2.8298 0.1831 10.1 2917.4043 2920.2927 6993.7 16 4.065 21.3 1 T.SFILQGATERSLAILDEIGRGTSGKEGI.S YGR198W 2 2 8.7% 817 95366 5.4 U YGR198W SGDID:S000003430, Chr VII from 894698-897151, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YGR198W is an essential gene" * Jamie_Ashy_SPB_70.25051.25051.4 3.0416 0.1066 17.5 4154.5366 4158.863 7826.3 29 3.277 17.2 1 K.KIKVNPSWYNFIISSMEKTFQSIEVSKTAMFLFQN.L * Jamie_Ashy_SPB_83.29655.29655.4 3.0423 0.2114 45.0 4188.2163 4191.5054 7729.9 92 3.806 17.1 1 S.NNLTSYLCNAMMLSTKEKDNADVEEQEEGEEEKALR.E YMR031C 5 5 8.7% 843 93345 6.3 U YMR031C SGDID:S000004633, Chr XIII from 334742-332211, reverse complement, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_Ashy_SPB_7.02526.02526.2 2.2297 0.011 84.4 1475.4722 1476.6726 8802.9 245 3.084 45.8 1 K.PKVYQTTGEPLSR.E * Jamie_Ashy_SPB_14.04924.04924.2 2.2519 0.1741 62.3 1517.3121 1517.8552 5654.2 2 3.563 54.2 1 E.PLSREALYKAKLK.Y * Jamie_Ashy_SPB_12.04327.04327.4 2.685 0.1227 15.9 3089.0166 3083.617 5425.0 128 3.186 19.8 1 M.NNIMAKVDAPKAQDLQKVKKVSAEKEAK.S * Jamie_Ashy_SPB_77.27751.27751.3 2.5481 0.0256 49.7 2282.6943 2279.7031 5328.7 108 3.41 27.4 1 K.KVSAEKEAKSMKFALGAANAVK.D * Jamie_Ashy_SPB_7.02370.02370.2 1.8301 0.1066 10.4 1268.6322 1266.4398 3903.0 216 3.008 50.0 1 A.QKRETYLSQL.T Reverse_YNL183C 3 3 8.7% 790 85990 8.4 U NPR1 SGDID:S000005127, Chr XIV from 295511-293139, reverse complement, Verified ORF, "Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation" * Jamie_Ashy_SPB_17.05978.05978.4 3.5288 0.0573 17.3 3303.6165 3306.7927 6038.7 19 3.362 19.9 1 T.RFEKVAFIKNDSIRQALKVSGGAGAGLDAGTK.I * Jamie_Ashy_SPB_19.06553.06553.4 3.5864 0.1081 13.0 3674.3765 3675.0989 7764.6 3 3.679 18.1 1 K.NDSIRQALKVSGGAGAGLDAGTKIYRKSFPIGSGGHS.H * Jamie_Ashy_SPB_41.14713.14713.3 2.774 0.1759 11.3 2577.5942 2577.8113 8171.8 4 3.699 28.4 1 T.TLTDASTRPSSNSTENKRKEQLL.R YGL192W 2 2 8.7% 600 69396 8.0 U IME4 SGDID:S000003160, Chr VII from 142252-144054, Verified ORF, "Probable mRNA N6-adenosine methyltransferase that is required for IME1 transcript accumulation and for sporulation; expression is induced in starved MATa/MAT alpha diploid cells" * Jamie_Ashy_SPB_25.08941.08941.2 1.6596 0.1401 20.4 1188.3922 1191.3292 5517.0 4 3.437 66.7 1 Q.FDQNLLVADR.P * Jamie_Ashy_SPB_64.22902.22902.4 2.6399 0.136 18.3 4872.2563 4875.308 8881.7 1 3.509 17.5 1 K.ISACQENHPGLIECIQSKIHFIPNIKPQTDISLGDCSYLDTC.H contaminant_INT-STD1 3 3 8.7% 607 69271 6.1 U BSA * Jamie_Ashy_SPB_25.08809.08809.2 1.9455 0.0666 88.3 1419.3722 1420.5713 5096.7 1 3.886 63.6 1 K.SLHTLFGDELCK.V * Jamie_Ashy_SPB_91.32545.32545.4 2.5631 0.0309 49.6 3324.7366 3322.823 6197.3 43 2.861 21.6 1 K.IETMREKVLASSARQRLRCASIQKFGER.A * Jamie_Ashy_SPB_49.17515.17515.2 2.9555 0.2964 100.0 1569.2522 1568.7258 7728.6 1 5.668 62.5 1 K.DAFLGSFLYEYSR.R Reverse_contaminant_KERATIN22 3 3 8.7% 645 65865 8.0 U no description * Jamie_Ashy_SPB_5.01634.01634.4 2.5127 0.1337 15.9 1733.6166 1733.7538 3439.2 1 3.684 31.0 1 F.GSGGGFGSGGGFGSGGGFGGGR.G * Jamie_Ashy_SPB_41.14568.14568.2 2.2441 0.3277 50.0 1327.4122 1329.4172 4751.5 1 5.761 46.7 1 G.GFGGGRGGFGGAAGFG.G * Jamie_Ashy_SPB_27.09440.09440.2 1.5551 0.0332 64.4 2111.872 2114.2449 5823.9 36 3.397 28.3 1 R.SGGSVVASGSSFGRFGGGGGGRGR.S Reverse_YGL064C 2 2 8.7% 561 63058 9.9 U MRH4 SGDID:S000003032, Chr VII from 383211-381526, reverse complement, Verified ORF, "Mitochondrial RNA helicase, plays an essential role in mitochondrial function" * Jamie_Ashy_SPB_27.09441.09441.3 2.7986 0.1628 10.5 2182.6143 2179.6353 5743.2 29 3.661 29.2 1 Q.KMRATRGVRHILDISTKPV.D * Jamie_Ashy_SPB_102.36670.36670.4 3.2967 0.1404 15.6 3550.4565 3553.1394 5069.0 348 3.838 19.0 1 K.SVTEYVQDVLEHTPVLIISRILVNKRLTEW.L Reverse_YGR189C 2 2 8.7% 507 52758 4.6 U CRH1 SGDID:S000003421, Chr VII from 878197-876674, reverse complement, Verified ORF, "Putative glycosidase of the cell wall, may have a role in cell wall architecture" * Jamie_Ashy_SPB_44.15655.15655.2 1.7117 0.1392 15.7 1516.8121 1515.528 5982.6 26 3.719 42.9 1 K.SSIQTTSSTVDNSST.A * Jamie_Ashy_SPB_61.21758.21758.3 3.0607 0.1506 10.1 3328.8843 3329.659 8128.7 18 3.52 22.3 1 G.TSYDTVIVKEIYMTFPADNYNTEGGAWEI.T Reverse_YEL056W 2 2 8.7% 401 45060 4.8 U HAT2 SGDID:S000000782, Chr V from 47168-48373, Verified ORF, "Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing" * Jamie_Ashy_SPB_35.12558.12558.2 1.9896 0.1664 42.7 1517.1122 1517.5463 5345.3 1 4.036 57.7 1 G.DEADDPTQEAGIQK.L * Jamie_Ashy_SPB_51.18403.18403.2 1.958 0.2668 10.0 2508.632 2507.7214 5585.8 1 4.441 35.0 1 D.ESVTGFLDKNFNHWKNDNIID.S Reverse_YMR097C 2 2 8.7% 367 42144 9.5 U MTG1 SGDID:S000004703, Chr XIII from 460526-459423, reverse complement, Verified ORF, "Peripheral GTPase of the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals" * Jamie_Ashy_SPB_21.07352.07352.2 2.9783 0.0649 45.7 1927.4722 1927.2072 5214.0 5 3.037 50.0 1 L.YLLYDAQFIPDVLNNK.V * Jamie_Ashy_SPB_28.09963.09963.2 1.8335 0.0299 74.1 1692.9521 1692.0145 7807.6 472 2.86 33.3 1 R.TVGAEAGTKAVKKFKR.G YLL003W 3 3 8.6% 946 112978 9.8 U SFI1 SGDID:S000003926, Chr XII from 143200-146040, Verified ORF, "Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C" * Jamie_Ashy_SPB_27.09724.09724.2 1.6577 0.11 17.5 1876.7522 1879.074 6781.2 48 3.312 36.7 1 N.IENEDKLGALYELENK.F * Jamie_Ashy_SPB_80.28513.28513.2 1.5761 0.1273 15.9 2199.2922 2200.691 5857.3 51 3.106 25.0 1 N.VHLLYTKLAPSMDRVKLSK.A * Jamie_Ashy_SPB_95.34003.34003.4 2.0668 0.063 43.2 5753.0566 5756.5693 5930.5 123 3.178 10.7 1 R.FCFYTEECNIQAISKRNYQLEKMVLKKFRERLLEIVKSEELADEVR.E Reverse_YER078C 2 2 8.6% 511 57990 6.5 U YER078C SGDID:S000000880, Chr V from 318338-316803, reverse complement, Uncharacterized ORF, "Metallopeptidase, localized to the mitochondrial matrix" * Jamie_Ashy_SPB_36.12920.12920.4 3.4595 0.1261 10.5 4066.6565 4069.6987 6573.7 85 3.708 15.2 1 S.VKPVDHVDLGLNHGIYHPYLKEVNWGSVKDIGLNKL.E * Jamie_Ashy_SPB_23.08206.08206.2 1.6008 0.2194 50.5 873.77216 874.9689 2598.4 66 4.257 57.1 1 K.EAFADKPP.V contaminant_gi|67568|pir||ELPG 2 2 8.6% 266 28821 8.1 U pancreatic elastase (EC 3.4.21.36) I precursor - pig * Jamie_Ashy_SPB_23.08052.08052.2 2.7953 0.4362 100.0 2171.9722 2172.316 6850.0 1 7.709 42.1 1 R.VVVGEHNLNQNDGTEQYVGV.Q * Jamie_Ashy_SPB_22.07759.07759.2 2.1517 0.2618 83.7 2541.3323 2541.78 7116.8 1 5.334 36.4 1 R.VVVGEHNLNQNDGTEQYVGVQKI.V Reverse_YNL224C 2 2 8.5% 767 86950 5.7 U YNL224C SGDID:S000005168, Chr XIV from 227100-224797, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_90.32323.32323.4 2.9149 0.0885 10.4 4705.0166 4709.3945 9450.3 328 3.981 14.6 1 L.TELTETDLALADDILLKKKKGKGKTELSKTEFTKFRETDHK.L * Jamie_Ashy_SPB_24.08625.08625.3 2.2715 0.0644 51.3 2621.1543 2619.9617 6604.7 258 2.99 19.6 1 K.RGQIGKANGRGAKKSNSGRAHRRR.S Reverse_YOL095C 2 2 8.5% 706 80576 8.6 U HMI1 SGDID:S000005455, Chr XV from 141346-139226, reverse complement, Verified ORF, "Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription" * Jamie_Ashy_SPB_31.11136.11136.3 2.4393 0.2146 11.0 2342.0344 2338.6665 3125.1 452 4.443 26.1 1 F.ELGKASHITSLKVTGPGTTQLAQ.Q * Jamie_Ashy_SPB_33.11857.11857.3 2.1193 0.0080 35.0 4430.8145 4429.0967 6316.6 27 3.6 16.7 1 K.EDSALSCVKLIDLLFQIRLDDMWDPQSKLKQYPIGYK.K Reverse_YJL034W 2 2 8.5% 682 74468 4.9 U KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p" Reverse_contaminant_GR78_YEAST 2 2 8.5% 682 74468 4.9 U owl|P16474| 78 KD GLUCOSE REGULATED PROTEIN HOMOLOG PRECURSOR (GRP 78) (IMMUNOGLOBULIN... Jamie_Ashy_SPB_2.00611.00611.2 1.997 0.1196 97.0 900.5722 901.0537 5479.4 1 3.682 85.7 1 K.KTPIATNR.K Jamie_Ashy_SPB_42.15018.15018.4 2.6264 0.234 35.4 5658.1367 5652.346 8003.8 370 4.778 11.2 1 K.QVSRDNYKLGILRKIDFITNQPNAAVQNKAADGILREDDTFAVYSPTIRN.G YJR122W 2 2 8.5% 497 57055 7.9 U CAF17 SGDID:S000003883, Chr X from 649691-651184, Verified ORF, "Mitochondrial protein that interacts with Ccr4p in the two-hybrid system; 3'-untranslated region contains a putative mRNA localization element common to genes encoding mitochondrial proteins" * Jamie_Ashy_SPB_99.35408.35408.2 2.3146 0.0474 15.8 1793.4922 1793.0757 7871.1 4 3.911 46.4 1 S.LFNSDPRILGIYVER.R * Jamie_Ashy_SPB_82.29319.29319.4 2.7854 0.0376 61.3 3122.4966 3121.4302 4033.9 344 3.171 19.9 1 K.YAEFHIDNVVEKSLAENEPTLNPFTNK.P YJL193W 2 2 8.5% 402 45937 9.5 U YJL193W SGDID:S000003729, Chr X from 71365-72573, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_27.09600.09600.4 2.9111 0.0939 15.1 2631.6565 2630.3752 5900.7 270 3.069 22.7 1 A.SIKTLSPMFILLLQKILKISTLK.I * Jamie_Ashy_SPB_16.05636.05636.2 1.7085 0.0566 13.0 1298.4321 1298.5717 6744.2 130 3.038 55.0 1 S.QSKIKAKIRPE.- Reverse_YER095W 2 2 8.5% 400 42963 4.9 U RAD51 SGDID:S000000897, Chr V from 349976-351178, Verified ORF, "Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein" * Jamie_Ashy_SPB_17.06151.06151.2 1.225 0.0025 60.1 1160.2322 1162.4565 6276.9 272 2.83 50.0 1 R.MFKALHMQR.A * Jamie_Ashy_SPB_43.15260.15260.3 2.9444 0.0901 12.6 2737.3743 2739.1472 8628.4 135 3.885 22.9 1 S.IGKIELLDKRPAYAVAEATHLGSER.L YKR095W 11 11 8.4% 1875 218454 5.2 U MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs" * Jamie_Ashy_SPB_68.24324.24324.4 3.093 0.0979 17.5 3119.5366 3116.5537 6577.5 172 3.333 22.7 1 K.FESVENEKMKLSSELEFVKRKLDDLT.E * Jamie_Ashy_SPB_12.04206.04206.2 1.7722 0.0466 75.4 2346.2522 2347.5474 4076.5 20 3.795 36.1 1 K.RKLDDLTEEKKETQSNQQR.T * Jamie_Ashy_SPB_12.04280.04280.2 6.0024 0.4893 100.0 2191.172 2191.3599 8706.7 1 9.42 67.6 1 R.KLDDLTEEKKETQSNQQR.T * Jamie_Ashy_SPB_25.08784.08784.2 3.0801 0.2191 100.0 1432.6721 1432.5303 6972.4 1 5.762 81.8 1 R.DLESQVQDLQTR.I * Jamie_Ashy_SPB_25.08962.08962.1 2.124 0.2533 100.0 1343.43 1343.4302 5462.9 1 4.908 59.1 1 K.DYESVITSVDSK.Q * Jamie_Ashy_SPB_20.07200.07200.2 2.5251 0.1185 98.1 1845.5322 1843.942 5915.2 2 3.615 43.3 1 K.DLYETTSQSLQQTNSK.L * Jamie_Ashy_SPB_24.08679.08679.3 3.799 0.2508 100.0 2116.0144 2116.336 5678.5 145 5.071 32.4 1 K.TLNLSRDQLENALKENEK.S * Jamie_Ashy_SPB_10.03476.03476.2 3.0857 0.401 100.0 1324.2522 1323.3586 5986.1 1 7.12 80.0 1 K.HEQLSSSDYEK.L * Jamie_Ashy_SPB_6.02059.02059.2 2.0848 0.1794 99.2 1643.1122 1644.738 4953.4 4 5.015 54.2 1 K.KWESEHEQEVSQK.I * Jamie_Ashy_SPB_39.14056.14056.3 1.8796 0.012 35.3 1492.9744 1494.5175 3847.3 45 2.504 33.3 1 K.DVPHSSHISDDER.D * Jamie_Ashy_SPB_19.06820.06820.3 2.9453 0.0602 75.5 2761.1943 2761.87 5559.6 1 3.099 27.2 1 K.RPIDEVGELKNDEDDTTENINESK.K Reverse_YJL098W 2 2 8.4% 1058 121402 4.4 U SAP185 SGDID:S000003634, Chr X from 241999-245175, Verified ORF, "Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p" * Jamie_Ashy_SPB_56.20142.20142.4 3.3401 0.0899 16.4 4058.7366 4056.973 4845.9 132 3.156 16.2 1 L.KDIVEEGDISTNASEVQNTDDEVEDDADGTNDDENEK.L * Jamie_Ashy_SPB_73.26260.26260.4 2.5392 0.1194 78.8 6087.6963 6090.2964 7022.6 16 3.786 12.1 1 R.RTDDHEELEHPLTVRTDESASDNEENELEEEEGSRTREKEEFTGDINKKDAK.A YGR089W 3 3 8.4% 936 106465 8.6 U NNF2 SGDID:S000003321, Chr VII from 656964-659774, Uncharacterized ORF, "Protein that exhibits physical and genetic interactions with Rpb8p, which is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation" * Jamie_Ashy_SPB_57.20348.20348.2 1.6332 0.0088 64.1 2332.0322 2330.6494 6135.3 307 3.165 25.0 1 R.QTAENKPLKKGFNSTKPDVAR.V * Jamie_Ashy_SPB_89.31748.31748.2 1.5764 0.1502 17.5 1846.4722 1846.9452 10916.4 151 3.273 31.2 1 K.EYKSYQPSVISAEGSNS.Q * Jamie_Ashy_SPB_34.12015.12015.4 2.3928 0.1081 74.6 4731.2563 4729.651 8325.8 20 3.376 15.0 1 K.FLSPLLTLKHSVLTTNANLSDERTKLKKTKKEFSKKLSLLR.Q YDR409W 2 2 8.4% 904 100796 6.9 U SIZ1 SGDID:S000002817, Chr IV from 1289396-1292110, Verified ORF, "SUMO ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring" * Jamie_Ashy_SPB_33.11635.11635.3 2.549 0.1715 13.1 3483.9844 3481.7112 7622.8 241 4.067 17.4 1 K.TSTTSSTSRAPPPTNPDASSSSSSFAVPTIHFKE.S * Jamie_Ashy_SPB_25.08944.08944.4 2.7014 0.1994 19.9 5078.0566 5073.6113 6978.1 37 3.925 15.0 1 K.HLQCFDALWFLHSQLQIPTWQCPVCQIDIALENLAISEFVDD.I YBR237W 2 2 8.4% 849 96359 8.1 U PRP5 SGDID:S000000441, Chr II from 691964-694513, Verified ORF, "RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA" * Jamie_Ashy_SPB_16.05570.05570.4 3.1515 0.1995 43.5 4143.2163 4141.6304 5442.9 280 3.803 16.2 1 K.SKRSKVKKKISFDFSDDDDSEIGVSFRSKEHIQKAP.E * Jamie_Ashy_SPB_100.35760.35760.3 1.6274 0.0239 33.5 3908.7244 3906.446 7246.3 200 2.747 15.4 1 K.HETGPMGLILAPTRELALQIHEEVTKFTEADTSIR.S YPL180W 3 3 8.4% 799 88845 8.1 U TCO89 SGDID:S000006101, Chr XVI from 205247-207646, Verified ORF, "Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin" * Jamie_Ashy_SPB_14.04896.04896.3 2.383 0.118 16.8 1514.5743 1518.7598 6578.3 23 3.851 43.8 1 P.RRSKSTQSVLSLR.D * Jamie_Ashy_SPB_19.06708.06708.2 1.9269 0.069 17.6 1760.3121 1758.95 6219.0 2 3.455 42.9 1 S.NSSRKSDSSFMSLRR.Q * Jamie_Ashy_SPB_103.36823.36823.4 2.299 0.0275 42.4 4446.4966 4440.8594 6054.2 9 3.221 16.2 1 K.DNNVPQHNFSTSISSLTNNLRRAAPESFHGSRMNNIFHK.K Reverse_YHR091C 3 3 8.4% 643 73694 9.4 U MSR1 SGDID:S000001133, Chr VIII from 286772-284841, reverse complement, Verified ORF, "Mitochondrial arginyl-tRNA synthetase" * Jamie_Ashy_SPB_10.03546.03546.1 1.059 0.0256 77.1 817.97 818.0067 5311.0 69 2.789 50.0 1 K.GSVWIKK.Y * Jamie_Ashy_SPB_98.35078.35078.3 1.314 0.0202 33.1 4245.474 4242.013 8161.2 98 2.681 14.9 1 K.SKMDQIIIASVGILDAVRQPNDVQSIKMKNNEMIQLAK.D * Jamie_Ashy_SPB_39.13808.13808.2 1.8136 0.0664 14.7 1120.5721 1120.2677 5320.2 5 4.352 56.2 1 T.DFSPMFGYR.P YGR197C 2 2 8.4% 547 61294 8.5 U SNG1 SGDID:S000003429, Chr VII from 894145-892502, reverse complement, Verified ORF, "Protein involved in nitrosoguanidine (MNNG) resistance; expression is regulated by transcription factors involved in multidrug resistance" * Jamie_Ashy_SPB_44.15873.15873.4 4.3331 0.2317 40.0 4040.7366 4041.253 8627.8 209 4.362 16.2 1 G.GRDGVSYSNQRFAEGSGHSSDLAKSLEDYRPPDEKPS.S * Jamie_Ashy_SPB_22.07752.07752.2 1.7488 0.0869 16.4 1102.1322 1101.2567 4972.7 1 3.715 75.0 1 G.FCTVSAIFR.I YGR056W 4 4 8.3% 928 106669 6.5 U RSC1 SGDID:S000003288, Chr VII from 601665-604451, Verified ORF, "One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook" * Jamie_Ashy_SPB_54.19186.19186.2 1.3317 0.1875 16.3 2797.0723 2797.1892 6101.7 108 3.975 22.0 1 T.PQPLASPTPVVNYANITSAHPKTHVR.R * Jamie_Ashy_SPB_36.12789.12789.2 2.2331 0.171 60.5 1818.7322 1817.0294 7168.5 64 3.782 32.1 1 K.NEVMKTGQYRDHPIQ.D * Jamie_Ashy_SPB_8.02684.02684.2 2.1972 0.034 19.7 832.2522 831.9444 4905.7 43 2.465 78.6 1 T.QLSSTAPK.K * Jamie_Ashy_SPB_44.15728.15728.2 1.301 0.1463 12.9 3200.7122 3198.7534 8510.5 5 3.757 22.2 1 K.YNFNHIVIEAPGAFVVPVPMEKNIRTIQ.S Reverse_YBL023C 2 2 8.3% 868 98780 5.1 U MCM2 SGDID:S000000119, Chr II from 177529-174923, reverse complement, Verified ORF, "Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex" * Jamie_Ashy_SPB_51.18373.18373.4 2.9687 0.162 16.3 5653.7363 5655.4395 7166.2 35 3.416 14.3 1 V.EEGPKSVDVLDALLIVERHRPLRGPPVTGPAEQLTVRQYNRYVTKEGNVR.F * Jamie_Ashy_SPB_39.13921.13921.3 2.5055 0.0951 75.1 2601.6543 2600.631 6523.5 115 3.62 22.6 1 R.FHQQPSSPPLENESDSDDEERR.R YNL268W 2 2 8.3% 611 68090 7.0 U LYP1 SGDID:S000005212, Chr XIV from 138551-140386, Verified ORF, "Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids" * Jamie_Ashy_SPB_50.17794.17794.3 3.0406 0.1589 10.6 3298.7644 3297.7056 7340.2 481 4.074 17.0 1 I.GFRYWRNPGAWGPGIISSDKSEGRFLGWV.S * Jamie_Ashy_SPB_27.09747.09747.2 1.4457 0.1596 16.0 2558.612 2561.1157 4783.2 202 3.622 26.2 1 T.LAGLCAWLFISLAHIRFMQALK.H YBR215W 2 2 8.3% 623 67517 9.2 U HPC2 SGDID:S000000419, Chr II from 653441-655312, Verified ORF, "Highly charged, basic protein required for normal cell-cycle regulation of histone gene transcription; mutants display strong synthetic defects with subunits of FACT, a complex that allows RNA Pol II to elongate through nucleosomes" * Jamie_Ashy_SPB_60.21402.21402.3 2.3836 0.1695 17.2 4163.724 4161.7427 7152.5 152 3.93 14.7 1 T.SSPVNLDVESTAQSLGKFNKSTNSLKAALTKAPAEKVSLK.R * Jamie_Ashy_SPB_3.00906.00906.2 2.0176 0.1013 22.4 1417.0122 1416.62 4587.3 124 3.273 50.0 1 P.LIEKGHYASLER.A YEL052W 2 2 8.3% 509 58265 8.5 U AFG1 SGDID:S000000778, Chr V from 56571-58100, Verified ORF, "Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family, localized to mitochondria" * Jamie_Ashy_SPB_100.35977.35977.3 1.4308 0.1545 43.0 4018.0444 4014.6194 4017.6 54 3.9 9.8 1 K.HRTKVIFLNSPTDYRKIPRPVSSVYYFPSDTSIK.Y * Jamie_Ashy_SPB_2.00572.00572.2 2.1057 0.0272 15.6 951.2922 951.15393 4745.2 36 2.176 64.3 1 G.FSKEIAKK.S YMR127C 2 2 8.3% 338 39206 9.2 U SAS2 SGDID:S000004734, Chr XIII from 523344-522328, reverse complement, Verified ORF, "Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family" * Jamie_Ashy_SPB_30.10803.10803.2 1.6774 8.0E-4 64.2 2533.132 2534.9805 4449.8 42 3.219 28.3 1 -.MARSLSQSLTATTQKLKGKKNGGK.G * Jamie_Ashy_SPB_50.17710.17710.2 2.0616 0.0418 12.8 1815.7122 1816.1575 6457.2 1 3.482 46.9 1 A.TTQKLKGKKNGGKGKNK.P YOR328W 5 5 8.2% 1564 176460 7.9 U PDR10 SGDID:S000005855, Chr XV from 931798-936492, Verified ORF, "ABC (ATP-binding cassette) membrane pump involved in the pleiotropic drug resistance network, regulated by Pdr1p and Pdr3p, similar to Pdr5p" * Jamie_Ashy_SPB_39.13891.13891.2 1.6323 0.0657 10.2 1762.1921 1762.0765 6290.8 11 3.298 39.3 1 I.TYQGTFGNMPIKYLK.M * Jamie_Ashy_SPB_46.16399.16399.4 2.9568 0.1183 16.8 4451.7764 4453.7046 7959.5 405 3.275 15.8 1 S.GPNYVNATGDEVTCSALGSIPGNNYVSGDDFIQTNYGYRHK.N * Jamie_Ashy_SPB_29.10338.10338.2 1.7714 0.1271 10.0 1290.7122 1290.4338 8252.2 12 3.465 54.5 1 Y.NEGAKQNGEMLV.F * Jamie_Ashy_SPB_76.27064.27064.4 3.0514 0.0219 65.2 4351.6567 4347.5996 7534.2 77 2.734 16.2 1 K.NQPTLSTSDAEKDVEMNNNSSATDSRFLRDSDAAIMGNDK.T * Jamie_Ashy_SPB_15.05253.05253.3 2.8441 0.1747 17.1 2513.7244 2513.7698 6962.4 2 3.765 30.0 1 V.RESLRFSAYLRQADDVSIEEK.D Reverse_YPL009C 3 3 8.2% 1038 119069 5.7 U YPL009C SGDID:S000005930, Chr XVI from 538933-535817, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_118.42409.42409.4 2.6617 0.0122 48.0 3114.6965 3114.6306 10375.9 394 2.532 21.7 1 K.KTFKLAQLRRQKERKNKRDERVER.K * Jamie_Ashy_SPB_30.10592.10592.4 3.2049 0.0656 19.3 2265.8564 2261.6328 4162.4 142 3.036 32.4 1 I.LHGKRENLEQVDLLAQIKR.D * Jamie_Ashy_SPB_55.19659.19659.4 2.78 0.1226 18.1 4908.177 4909.6606 6487.2 16 3.597 15.9 1 R.QLAMIRRNEDLLIVNGASFFELVLYFHGDAFQLVLIRDQDVQ.K Reverse_YDL185W 4 4 8.2% 1071 118637 6.2 U TFP1 SGDID:S000002344, Chr IV from 126788-130003, Verified ORF, "Vacuolar ATPase V1 domain subunit A containing the catalytic nucleotide binding sites; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease" * Jamie_Ashy_SPB_52.18652.18652.4 3.6242 0.0489 17.2 4620.7764 4625.152 7789.1 76 3.499 14.6 1 K.WIPCFADYTSYGNQQLFDEKILTAVDLTIKDSDSLASKGVL.Q * Jamie_Ashy_SPB_25.09006.09006.2 2.3103 0.0582 17.8 2130.632 2131.477 6006.8 29 3.99 30.6 1 E.PGESIPYSKSVEKVLEVIR.G * Jamie_Ashy_SPB_106.37891.37891.4 3.011 0.1105 16.7 4108.6167 4113.805 6109.0 11 3.589 17.1 1 Y.SKSVEKVLEVIRGDPAKKQGMEFTIVEFYEVGKITR.S * Jamie_Ashy_SPB_22.07681.07681.4 2.6966 0.1212 15.2 4223.4165 4224.955 6675.8 174 3.483 16.2 1 V.EKVLEVIRGDPAKKQGMEFTIVEFYEVGKITRSLRR.V YGR178C 3 3 8.2% 722 78782 6.9 U PBP1 SGDID:S000003410, Chr VII from 853220-851052, reverse complement, Verified ORF, "Protein interacting with poly(A)-binding protein Pab1p; likely involved in control of the extent of mRNA polyadenylation; also interacts with Mkt1p to form a complex that may regulate translation of the HO mRNA" * Jamie_Ashy_SPB_31.11007.11007.2 1.9615 0.0386 13.8 1336.1322 1335.466 6937.8 104 3.106 50.0 1 F.RKRDSSTNSRK.G * Jamie_Ashy_SPB_15.05130.05130.4 2.7947 0.1643 16.8 2368.5366 2372.683 6763.3 15 3.638 28.9 1 K.KFGVKSTFDEHLYTTKINKD.D * Jamie_Ashy_SPB_46.16262.16262.2 1.7326 0.0855 12.2 3005.4521 3008.2224 9577.7 50 3.544 20.4 1 R.GIIIDDSGLDEEDLYSGVDRRGDELLAA.L Reverse_YGL125W 2 2 8.2% 600 68560 5.8 U MET13 SGDID:S000003093, Chr VII from 272526-274328, Verified ORF, "Isozyme of methylenetetrahydrofolate reductase, catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway" * Jamie_Ashy_SPB_17.05884.05884.4 2.6855 0.1247 17.1 3927.4165 3925.3008 7090.5 20 4.004 18.6 1 V.ANSKSNDPHNSLLDGQSDIAFYTLFENNKLTDILK.P * Jamie_Ashy_SPB_58.20582.20582.4 2.9745 0.1943 94.6 5242.8164 5242.996 7435.0 91 4.45 13.3 1 K.SNDPHNSLLDGQSDIAFYTLFENNKLTDILKPLKTKPLMFELYQK.Q Reverse_YOR113W 3 3 8.1% 914 101170 7.4 U AZF1 SGDID:S000005639, Chr XV from 534075-536819, Verified ORF, "Zinc-finger transcription factor, involved in induction of CLN3 transcription in response to glucose; genetic and physical interactions indicate a possible role in mitochondrial transcription or genome maintenance" * Jamie_Ashy_SPB_79.28309.28309.4 3.0161 0.1345 12.2 3747.3765 3747.163 6825.7 77 3.254 17.1 1 N.AIANNANTNPNLITSAPHNEPSSITSKKTGVGKGRGK.I * Jamie_Ashy_SPB_54.19323.19323.3 2.6332 0.1165 16.7 2169.3843 2170.619 9861.9 192 3.741 23.7 1 K.RAQIILMLQDVKTAGILNTA.D * Jamie_Ashy_SPB_14.04942.04942.2 1.9866 0.0396 81.5 1852.8922 1855.1626 6329.1 1 3.387 40.6 1 K.TTAAPILLPPRMSNSER.R Reverse_YJR059W 3 3 8.1% 818 91400 8.9 U PTK2 SGDID:S000003820, Chr X from 545701-548157, Verified ORF, "Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake" * Jamie_Ashy_SPB_54.19464.19464.3 2.0435 0.0342 37.8 3896.6343 3899.4688 5959.8 82 2.838 17.5 1 K.SCRKYFKEPSEHYIMNLKKLAYVDKQRYSSK.V * Jamie_Ashy_SPB_13.04545.04545.4 3.1028 0.0765 12.1 3102.4966 3098.279 5096.8 6 2.87 21.0 1 K.RRHLSASRQRAGASSSPSNGGSGSGSISPSTP.R * Jamie_Ashy_SPB_91.32574.32574.2 1.3852 0.1176 76.0 2290.7322 2290.4116 8965.9 101 3.647 25.0 1 R.AGASSSPSNGGSGSGSISPSTPRKR.L Reverse_YBR131W 3 3 8.1% 704 80732 5.3 U CCZ1 SGDID:S000000335, Chr II from 497157-499271, Verified ORF, "Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway" * Jamie_Ashy_SPB_12.04042.04042.3 2.8412 0.1159 18.1 2370.8643 2368.5222 6538.5 191 3.629 22.7 1 K.QTKDNVGNGFGETTNKKSIANSG.G * Jamie_Ashy_SPB_1.00381.00381.3 2.6895 0.158 12.3 2690.9343 2695.1338 4711.1 27 3.873 23.9 1 L.NLKLAQYSSPLLDSNLPSILFGFR.S * Jamie_Ashy_SPB_32.11545.11545.2 1.4024 0.1247 13.2 1094.7522 1094.1204 3718.2 206 3.152 50.0 1 R.ESLSLESTNN.Q YOL095C 2 2 8.1% 706 80576 8.6 U HMI1 SGDID:S000005455, Chr XV from 141346-139226, reverse complement, Verified ORF, "Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription" * Jamie_Ashy_SPB_20.07117.07117.4 2.8222 0.1832 11.1 5083.9766 5086.7603 7516.3 126 4.019 14.7 1 E.NIKPEEILIFSLTNKAVDSIIENLLSIFENSHTNKEIVHQIGCY.T * Jamie_Ashy_SPB_33.11625.11625.3 2.2114 0.136 14.9 1592.6943 1592.817 4435.6 375 3.03 35.4 1 D.NCKVMTNDDLIIR.A YKL155C 3 3 8.1% 628 72190 9.4 U RSM22 SGDID:S000001638, Chr XI from 161347-159461, reverse complement, Verified ORF, "Mitochondrial ribosomal protein of the small subunit" * Jamie_Ashy_SPB_73.26022.26022.2 2.1134 0.2489 22.2 1562.2122 1561.7991 5500.8 3 4.736 50.0 1 I.ERGNPMGFEIIARA.R * Jamie_Ashy_SPB_100.35763.35763.2 1.8644 0.1686 29.1 1669.9122 1668.7136 6058.2 20 4.159 39.3 1 K.YDIGSLGDDTQNSWP.R * Jamie_Ashy_SPB_27.09582.09582.3 2.1839 0.045 43.5 2533.1343 2532.9014 6849.5 29 3.162 29.8 1 R.KGHVMMDLCAPSGELEKWTVSR.S YBR149W 2 2 8.1% 344 38884 6.0 U ARA1 SGDID:S000000353, Chr II from 539981-541015, Verified ORF, "Large subunit of NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; small subunit is unidentified" * Jamie_Ashy_SPB_18.06408.06408.3 1.9991 0.0754 51.7 1594.7344 1598.796 5682.9 38 3.174 30.8 1 R.AIGVSNFSIEYLER.L * Jamie_Ashy_SPB_22.07875.07875.3 1.8885 0.019 35.4 1547.1843 1550.8447 4840.3 97 2.672 34.6 1 R.QGTIVIPRSLNPVR.I YER161C 2 2 8.1% 333 38551 9.4 U SPT2 SGDID:S000000963, Chr V from 500343-499342, reverse complement, Verified ORF, "Protein involved in negative regulation of transcription, exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins" * Jamie_Ashy_SPB_16.05527.05527.2 2.0607 0.0254 11.5 2183.9321 2184.501 6763.4 26 3.32 26.3 1 L.SQIRKSTTASKAQVQDPLPK.K * Jamie_Ashy_SPB_5.01502.01502.1 1.7917 0.1486 100.0 840.58 840.0537 3447.6 115 3.539 58.3 1 R.KKPEPIK.K YPL115C 3 3 8.0% 1128 124913 6.8 U BEM3 SGDID:S000006036, Chr XVI from 335485-332099, reverse complement, Verified ORF, "Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly" * Jamie_Ashy_SPB_73.26239.26239.3 2.5187 0.2248 25.5 3553.0444 3554.1216 8738.0 29 4.054 18.9 1 R.KAHIKSLNLPIPTLSPPLQQGSDVALETSVTPTV.P * Jamie_Ashy_SPB_66.23727.23727.3 2.7581 0.1362 14.5 2182.6443 2178.3635 4765.9 105 3.853 30.3 1 K.QKQLGETASIHSTNTLNTFS.S * Jamie_Ashy_SPB_58.20835.20835.3 2.2868 0.0991 67.1 3822.4744 3824.2207 11168.3 129 3.366 14.3 1 K.KLMEVSSSSNSSTGPHVSTAIFGSSLETCLRLSSHK.Y Reverse_YGR258C 3 3 8.0% 1031 117838 5.2 U RAD2 SGDID:S000003490, Chr VII from 1010772-1007677, reverse complement, Verified ORF, "Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein" * Jamie_Ashy_SPB_11.03940.03940.2 1.2816 0.0296 61.8 2240.872 2238.6067 5936.5 109 3.327 25.0 1 K.FNKLNGFEAIVEISSVPGMGK.L * Jamie_Ashy_SPB_24.08484.08484.4 2.2263 0.0056 36.4 4921.0967 4918.485 5004.0 77 2.817 12.9 1 K.FIQVSSGSSPTSNKVNDNSGNQLQLALLKRATSKASERKGQRREK.R * Jamie_Ashy_SPB_69.24667.24667.2 1.7659 0.0861 14.0 2127.872 2129.4702 7808.7 172 3.292 32.4 1 R.EKRQRITERKLVPVGGDF.V YJR061W 2 2 8.0% 935 108427 6.6 U YJR061W SGDID:S000003822, Chr X from 550425-553232, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_66.23755.23755.4 2.7537 0.146 15.3 4737.6567 4735.844 7936.1 56 3.707 15.8 1 H.FKKIIITLLTIQLLFITIFVLGGRSSIIDGNWKSFMALFFK.P * Jamie_Ashy_SPB_90.32335.32335.4 2.8911 0.1112 19.4 3578.8564 3579.859 5192.4 2 3.755 20.7 1 V.HGNGENNIDARFIDIDSGIYIDITGLSVSSDAAK.Q Reverse_YDR006C 2 2 8.0% 901 101082 9.1 U SOK1 SGDID:S000002413, Chr IV from 461244-458539, reverse complement, Verified ORF, "Protein whose overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13" * Jamie_Ashy_SPB_87.31032.31032.4 2.5133 0.0224 45.4 3274.4165 3268.4932 5775.0 288 2.773 20.4 1 K.AFTIKSNDLSPLQPRRAQECSGNNDYER.H * Jamie_Ashy_SPB_73.26173.26173.4 3.4484 0.1374 18.6 4524.0166 4521.955 9225.2 4 3.568 16.3 1 S.TDGSKMDNKLAAAQTKAIADGDPSDKSPVVSSPKSLSSSSREQK.S Reverse_YGL120C 2 2 8.0% 767 87562 6.4 U PRP43 SGDID:S000003088, Chr VII from 283943-281640, reverse complement, Verified ORF, "RNA helicase in the DEAH-box family, involved in release of the lariat-intron from the spliceosome" * Jamie_Ashy_SPB_55.19693.19693.4 3.0524 0.0131 59.5 4124.0166 4122.798 4649.7 72 2.856 16.2 1 R.IRPNYVKQKSFGPDVVYVIGDITLSTEAINTSIVVKR.G * Jamie_Ashy_SPB_45.16242.16242.4 3.1156 0.1533 15.8 2657.4966 2656.0283 4994.6 111 3.777 23.2 1 R.GPVALLPADNFYRQFKEADLTASM.I Reverse_contaminant_KERATIN14 2 2 8.0% 638 65871 8.1 U no description * Jamie_Ashy_SPB_88.31669.31669.2 1.8388 0.125 10.4 2044.6122 2047.0623 6617.6 495 3.927 22.7 1 I.SGAGGLRSGSSSSQYSGSSGRGG.T * Jamie_Ashy_SPB_85.30376.30376.4 2.5936 0.1181 80.1 2991.3765 2993.2935 5074.9 48 3.209 22.2 1 R.SSGSVVASRGSFGQSRGSFSKKCVQRNM.- YGL056C 2 2 8.0% 527 58112 9.0 U SDS23 SGDID:S000003024, Chr VII from 397624-396041, reverse complement, Verified ORF, "One of two S. cerevisiae homologs (Sds23p and Sds24p) of the Schizosaccharomyces pombe Sds23 protein, which genetic studies have implicated in APC/cyclosome regulation" * Jamie_Ashy_SPB_91.32788.32788.2 1.0601 0.0655 60.7 2745.0923 2743.135 6390.4 47 3.508 20.8 1 K.NPFYKLPETENLSTVIGILGSGVHR.V * Jamie_Ashy_SPB_86.30865.30865.2 1.7845 0.0488 10.7 1941.1322 1940.2992 5611.4 43 3.202 31.2 1 C.RHFVSVILNLRGLETGK.D Reverse_YBR168W 2 2 8.0% 413 48579 8.7 U PEX32 SGDID:S000000372, Chr II from 572366-573607, Verified ORF, "Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p" * Jamie_Ashy_SPB_49.17560.17560.3 1.9467 0.0399 36.8 3685.2244 3684.5054 7699.8 328 3.275 15.6 1 K.PLVEVLPVTIARGQSLPIIQEMKLHKPLFSSIK.F * Jamie_Ashy_SPB_12.04260.04260.4 3.2334 0.0438 85.4 1622.3765 1622.9666 5348.3 132 3.05 32.1 1 R.GQSLPIIQEMKLHK.P Reverse_YHR068W 2 2 8.0% 387 42892 5.8 U DYS1 SGDID:S000001110, Chr VIII from 232135-233298, Verified ORF, "Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric" * Jamie_Ashy_SPB_87.31296.31296.2 2.3993 0.1454 55.9 1771.0521 1773.1722 9637.2 185 4.036 34.4 1 -.NKVKKIPKGSAFTAAVI.L * Jamie_Ashy_SPB_70.25206.25206.2 1.5146 0.0461 66.7 1690.6721 1691.8375 6699.3 73 3.16 34.6 1 K.HAWYLVSSEDNIEK.G Reverse_YFR018C 2 2 8.0% 363 41031 5.3 U YFR018C SGDID:S000001914, Chr VI from 184210-183119, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_120.43211.43211.2 1.3155 0.1804 15.1 1649.2322 1649.8658 3167.4 50 3.607 25.0 1 A.CSAASDIAGVMGTPAIK.T * Jamie_Ashy_SPB_32.11266.11266.2 1.477 0.02 64.2 1312.4722 1315.4288 5524.0 281 3.129 45.5 1 R.QIERSGESGPVR.T Reverse_YDR356W 5 5 7.9% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * Jamie_Ashy_SPB_50.17953.17953.4 3.0026 0.0851 12.4 4510.6567 4511.07 6261.2 365 3.389 15.7 1 K.LKYYSIIDNLQMTSDNDKRRENLILREELRRNEEQL.S * Jamie_Ashy_SPB_18.06313.06313.2 1.4391 0.0056 62.0 1439.2522 1441.6737 7006.0 44 2.655 50.0 1 R.RENLILREELR.R * Jamie_Ashy_SPB_13.04558.04558.1 1.5802 0.0395 85.2 1000.55 1001.174 2848.7 1 3.095 71.4 1 K.RNLISIER.E * Jamie_Ashy_SPB_52.18422.18422.2 1.5658 0.069 77.0 1262.3322 1259.4513 6357.8 72 2.919 44.4 1 K.LRKSENQKQK.L * Jamie_Ashy_SPB_84.30110.30110.4 2.787 0.0257 55.1 3546.2966 3546.0366 6731.4 104 3.017 20.2 1 R.KSENQKQKLQLDMEDSKIKLENLENTRKR.L Reverse_YMR257C 2 2 7.9% 800 94523 8.8 U PET111 SGDID:S000004870, Chr XIII from 782030-779628, reverse complement, Verified ORF, "Specific translational activator for the COX2 mRNA, located in the mitochondrial inner membrane" * Jamie_Ashy_SPB_96.34323.34323.3 2.0521 0.1252 66.1 4167.7744 4171.885 9938.6 27 3.727 17.2 1 K.KNEEFLKMKRENQELKLLSIEEDFWEKLFLTLK.R * Jamie_Ashy_SPB_62.22322.22322.4 3.7111 0.1055 15.8 3886.0566 3885.6365 6718.7 36 3.672 19.5 1 S.LKEFSRYNSIIEWKLWLIDVKLPIYRFRTR.L YLL001W 2 2 7.9% 757 84972 5.5 U DNM1 SGDID:S000003924, Chr XII from 147889-150162, Verified ORF, "Dynamin-related GTPase required for mitochondrial fission and the maintenance of mitochondrial morphology, assembles on the cytoplasmic face of mitochondrial tubules at sites at which division will occur; also participates in endocytosis" * Jamie_Ashy_SPB_37.13029.13029.4 3.0568 0.167 18.4 2688.0166 2688.9048 5651.6 55 3.571 23.9 1 N.LQISDFSLGDIDDLENAEPPLTER.E * Jamie_Ashy_SPB_65.23170.23170.3 1.8637 0.017 33.3 4364.9644 4362.034 8787.7 63 3.143 14.3 1 R.LIVSYFDIIREMIEDQVPKAVMCLLVNYCKDSVQNR.L Reverse_YAL010C 2 2 7.9% 493 56251 7.3 U MDM10 SGDID:S000000008, Chr I from 135667-134186, reverse complement, Verified ORF, "Subunit of the mitochondrial sorting and assembly machinery (SAM complex); has a role in assembly of the TOM complex, which mediates protein import through the outer membrane; required for normal mitochondrial morphology and inheritance" * Jamie_Ashy_SPB_53.19033.19033.3 2.2732 0.0053 38.3 2686.9143 2688.0557 8132.3 321 3.272 22.7 1 K.DRLSTSLKWVSTFISSNIKNDFK.E * Jamie_Ashy_SPB_43.15333.15333.2 1.925 0.2113 60.2 1882.0322 1882.086 7441.3 493 4.402 30.0 1 L.TLTLPRGTNTSHTYYR.L YDR524C 2 2 7.9% 482 54494 9.5 U AGE1 SGDID:S000002932, Chr IV from 1488980-1487532, reverse complement, Verified ORF, "ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif" * Jamie_Ashy_SPB_33.11862.11862.2 1.508 0.107 11.7 1268.7522 1266.4126 7464.4 2 3.121 61.1 1 R.KIDKSNLKCC.D * Jamie_Ashy_SPB_23.08242.08242.4 2.0359 0.0025 33.4 4013.7766 4013.377 6570.6 470 2.535 16.7 1 K.SNLKCCDCGSTATVEWVSINLLCILCIKCSGVHR.S Reverse_YER061C 2 2 7.9% 442 47640 8.3 U CEM1 SGDID:S000000863, Chr V from 279624-278296, reverse complement, Verified ORF, "Mitochondrial beta-keto-acyl synthase with possible role in fatty acid synthesis; required for mitochondrial respiration" * Jamie_Ashy_SPB_49.17379.17379.2 1.5915 0.0050 63.4 2300.0923 2298.6938 8777.0 199 2.786 23.8 1 R.AGLLHGIAGKNSSIYLPKSKSR.G * Jamie_Ashy_SPB_15.05396.05396.3 2.2478 0.1627 15.8 1383.1743 1383.5714 7205.2 6 3.81 47.9 1 V.IMGCGEGLVFGSR.Q Reverse_YKL113C 2 2 7.9% 382 43279 8.9 U RAD27 SGDID:S000001596, Chr XI from 225519-224371, reverse complement, Verified ORF, "5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family" * Jamie_Ashy_SPB_118.42298.42298.2 1.8803 0.0703 17.3 1455.9521 1457.7164 8615.4 6 3.138 42.3 1 F.GDLRGQIGSKLGKK.L * Jamie_Ashy_SPB_118.42400.42400.2 1.5075 0.1289 13.6 1950.5322 1952.224 6908.6 299 3.977 33.3 1 L.KQAEENHEKSVKVLRR.E YNL075W 2 2 7.9% 290 33482 9.0 U IMP4 SGDID:S000005019, Chr XIV from 485608-486480, Verified ORF, "Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs" * Jamie_Ashy_SPB_1.00399.00399.2 1.7781 0.101 16.3 1132.0322 1131.2339 5075.7 9 3.48 68.8 1 L.QDSQLQQKR.Q * Jamie_Ashy_SPB_118.42177.42177.2 1.7941 0.0689 12.7 1599.7322 1599.8307 7176.9 18 3.493 42.3 1 D.PRIIVTTSRDPSTR.L YGR204W 3 3 7.8% 946 102205 6.8 U ADE3 SGDID:S000003436, Chr VII from 905939-908779, Verified ORF, "Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine" * Jamie_Ashy_SPB_89.31801.31801.3 3.0155 0.1901 11.1 3380.0344 3375.9167 8873.2 302 4.277 17.5 1 G.FKCVGDVEFNEAIKYVHLITPVPGGVGPMTV.A * Jamie_Ashy_SPB_91.32764.32764.4 2.3596 0.1624 19.6 3387.8564 3388.8665 6780.9 15 3.597 19.4 1 Q.VIPMDEFNLHLTGDIHAIGAANNLLAAAIDTR.M * Jamie_Ashy_SPB_22.07894.07894.2 1.8086 0.0554 12.0 1299.2122 1300.5182 4585.9 16 3.152 60.0 1 F.TMGGERFFNIK.C YLR045C 2 2 7.8% 888 100918 8.6 U STU2 SGDID:S000004035, Chr XII from 237704-235038, reverse complement, Verified ORF, "Microtubule-associated protein (MAP) of the XMAP215/Dis1 family; regulates microtubule dynamics during spindle orientation and metaphase chromosome alignment; interacts with spindle pole body component Spc72p" * Jamie_Ashy_SPB_91.32613.32613.3 3.0515 0.1893 33.0 2564.0344 2560.8074 5735.5 89 4.199 23.9 1 R.KSILSNDEGEYQIDKDGDTLMGM.E * Jamie_Ashy_SPB_101.36208.36208.4 1.7602 0.0021 33.7 5625.5767 5631.4404 8639.6 15 3.119 11.1 1 R.NEDMLKDILEHMKHKTPQIRMECTQLFNASMKEEKDGYSTLQRYLK.D YNL082W 3 3 7.8% 873 99355 6.3 U PMS1 SGDID:S000005026, Chr XIV from 473392-476013, Verified ORF, "ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL" * Jamie_Ashy_SPB_11.03694.03694.2 1.5856 0.1443 89.5 2098.392 2099.4807 4415.1 314 4.139 31.2 1 R.QFIYVNKRPVEYSTLLK.C * Jamie_Ashy_SPB_116.41575.41575.2 1.0039 0.0076 60.3 2408.672 2408.8645 3964.2 9 2.999 17.5 1 K.MEVVGQFNLGFIIVTRKVDNK.Y * Jamie_Ashy_SPB_88.31688.31688.4 2.7791 0.102 11.3 3405.3364 3405.0557 5111.4 18 3.029 21.8 1 F.KSQKLIIPQPVELSVIDELVVLDNLPVFEK.N Reverse_YGL236C 2 2 7.8% 669 74215 8.3 U MTO1 SGDID:S000003205, Chr VII from 55796-53787, reverse complement, Verified ORF, "Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants" * Jamie_Ashy_SPB_78.28123.28123.4 3.3992 0.1051 11.7 4384.5767 4389.2397 7192.9 301 3.594 15.4 1 V.PQSPKRAVRYLEFLAAATIGQIRRAQGITLPQVRNLLLK.C * Jamie_Ashy_SPB_48.17100.17100.2 1.9059 0.0237 10.9 1372.7322 1372.6047 5707.9 152 3.199 45.8 1 V.QTGDDLVVGKIVK.H Reverse_YER129W 3 3 7.7% 1142 126872 8.7 U SAK1 SGDID:S000000931, Chr V from 417277-420705, Verified ORF, "Upstream kinase for the SNF1 complex; partially redundant function with Elm1p and Tos3p; members of this family of kinases have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome" * Jamie_Ashy_SPB_59.21094.21094.4 2.2413 0.1539 79.8 6204.5767 6201.994 7850.8 116 3.382 11.4 1 K.SISIPEFQDTQNCQFRLKQELVSSLLKEDNEPVHDFDWSVFPHKKIAPITMR.K * Jamie_Ashy_SPB_30.10551.10551.2 1.3273 0.0129 61.1 2969.8123 2972.3438 5893.4 199 3.048 20.8 1 K.LLLDKAMEYEEECSVKSVKNSQIEK.F * Jamie_Ashy_SPB_4.01182.01182.2 1.9526 0.064 16.0 1107.0721 1107.1655 4342.9 8 3.258 65.0 1 D.VSSSAISANDR.A YDL116W 3 3 7.7% 726 83635 4.8 U NUP84 SGDID:S000002274, Chr IV from 251566-253746, Verified ORF, "Subunit of the nuclear pore complex (NPC), forms a subcomplex with Nup85p, Nup120p, Nup145p-C, Sec13p, and Seh1p that plays a role in nuclear mRNA export and NPC biogenesis" * Jamie_Ashy_SPB_9.03127.03127.2 1.6244 0.0679 10.7 1754.4521 1753.02 6849.2 277 3.396 34.6 1 F.NTQQGIKKHSLWRR.T * Jamie_Ashy_SPB_30.10513.10513.2 1.9342 0.0762 15.7 2055.1921 2057.4097 7865.3 1 4.187 43.8 1 I.NFLRLPASNILRRTTQR.V * Jamie_Ashy_SPB_20.07245.07245.3 2.5529 0.1966 10.3 2994.9243 2992.4722 6311.6 1 4.943 26.0 1 A.LSRRFLLNGRVKALEQFMERNNIGE.I YMR301C 2 2 7.7% 690 77522 9.6 U ATM1 SGDID:S000004916, Chr XIII from 869626-867554, reverse complement, Verified ORF, "Mitochondrial inner membrane transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol; member of the ATP-binding cassette (ABC) transporter family" * Jamie_Ashy_SPB_117.42030.42030.2 1.7302 0.0613 13.4 2163.5923 2166.352 9784.1 440 3.086 25.0 1 M.FFDEATSALDTHTEQALLR.T * Jamie_Ashy_SPB_85.30532.30532.3 2.1837 0.1977 16.3 3833.0044 3834.3274 7233.7 343 3.615 15.9 1 R.TIRDNFTSGSRTSVYIAHRLRTIADADKIIVLDN.G contaminant_KERATIN14 2 2 7.7% 638 65871 8.1 U no description * Jamie_Ashy_SPB_67.23833.23833.4 2.7245 0.1492 15.2 3460.2166 3455.5688 5026.3 11 3.953 17.9 1 R.AGGFGGGRSSCGFAGGYGGGFGGSYGGGFGGGRGVGSGFG.G Jamie_Ashy_SPB_5.01832.01832.2 2.1983 0.1982 81.2 1194.6921 1195.3176 4523.2 20 4.61 75.0 1 K.KYEDEINKR.T YLR231C 2 2 7.7% 453 51032 5.3 U BNA5 SGDID:S000004221, Chr XII from 607121-605760, reverse complement, Verified ORF, "Kynureninase, required for biosynthesis of nicotinic acid from tryptophan via kynurenine pathway" * Jamie_Ashy_SPB_3.01111.01111.2 1.8732 0.0412 16.2 893.27216 893.9719 6405.9 99 3.419 75.0 1 G.EYPESLR.D * Jamie_Ashy_SPB_50.17812.17812.3 3.0489 0.1657 18.2 3055.1343 3058.566 9232.3 4 3.483 20.4 1 Q.MLEVFEPIPGALGFRQSNPSVIDTVALR.S YBR039W 2 2 7.7% 311 34351 9.3 U ATP3 SGDID:S000000243, Chr II from 315575-316510, Verified ORF, "Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis" * Jamie_Ashy_SPB_22.07716.07716.4 2.3679 0.0507 56.6 1902.6965 1907.0934 4333.4 102 2.902 32.3 1 R.RHLNDQPNADIVTIGDK.I * Jamie_Ashy_SPB_93.33310.33310.2 1.9114 0.1132 18.8 2025.4922 2026.4454 6983.0 114 3.753 29.4 1 Q.PNADIVTIGDKIKMQLLR.T YPR049C 3 3 7.6% 1178 135025 5.7 U ATG11 SGDID:S000006253, Chr XVI from 664670-661134, reverse complement, Verified ORF, "Peripheral membrane protein required for delivery of aminopeptidase I (Lap4p) to the vacuole in the cytoplasm-to-vacuole targeting pathway; also required for peroxisomal degradation (pexophagy)" * Jamie_Ashy_SPB_29.10170.10170.4 3.1627 0.087 16.6 2520.8965 2518.7893 5164.4 80 3.104 26.7 1 R.EIDENASFRDIITVNIDRLRQ.M * Jamie_Ashy_SPB_70.24961.24961.4 2.3886 0.0146 41.6 6049.9766 6047.77 7840.9 425 2.851 11.9 1 K.FELEDQMAESFEVLVSEMRTRSRNVLDKEEEEFNSQEFLKSMNVMLEKDK.K * Jamie_Ashy_SPB_21.07448.07448.3 2.8398 0.1499 13.2 1943.4844 1945.2266 6109.7 19 3.606 30.9 1 R.RVKGLKKGTAQSNILDES.T Reverse_YDR359C 3 3 7.6% 982 112502 9.0 U VID21 SGDID:S000002767, Chr IV from 1194875-1191927, reverse complement, Verified ORF, "Component of the NuA4 histone acetyltransferase complex" * Jamie_Ashy_SPB_87.31292.31292.4 2.8996 0.1342 14.4 4614.9766 4611.145 7594.9 65 3.667 14.9 1 S.YTLRHTMHASILEWNYGYTNINKVLEQDDESLWITPTR.N * Jamie_Ashy_SPB_54.19362.19362.4 3.6028 0.1103 10.1 4013.6965 4010.6152 7427.5 93 3.706 16.7 1 E.KKSEETEILASSAAVEHQILVVESSSSPKKLLLKTDI.S * Jamie_Ashy_SPB_70.25212.25212.3 3.0516 0.1134 16.1 3079.0745 3079.6047 7114.7 416 3.318 18.8 1 E.TEILASSAAVEHQILVVESSSSPKKLLLK.T YDR499W 3 3 7.6% 747 86425 6.0 U LCD1 SGDID:S000002907, Chr IV from 1447820-1450063, Verified ORF, "Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP" * Jamie_Ashy_SPB_80.28686.28686.3 2.5195 0.1699 15.0 2945.9043 2944.491 7966.5 22 3.928 21.9 1 I.QAVKVNELQVKHLQELAKLKQELQK.L * Jamie_Ashy_SPB_65.23238.23238.2 1.9697 0.0819 16.4 2143.4321 2143.364 6542.3 35 3.572 28.1 1 K.DHFKQLREEQIKQVESQ.L * Jamie_Ashy_SPB_67.23994.23994.2 1.7629 0.0572 77.7 1915.3522 1917.2483 6590.7 207 3.417 32.1 1 R.IMQVKYDEKFQEMAR.T YDR398W 2 2 7.6% 643 72002 4.9 U UTP5 SGDID:S000002806, Chr IV from 1267461-1269392, Verified ORF, "Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA" * Jamie_Ashy_SPB_77.27495.27495.3 1.9146 0.015 33.3 3704.9343 3706.0552 10053.2 101 2.656 15.4 1 K.ELSHSGQADSIAVTTEDGSLEIFVDPLVSSSTKKR.G * Jamie_Ashy_SPB_113.40423.40423.2 1.6713 0.1403 91.1 1566.9922 1564.912 4641.0 51 3.359 42.3 1 K.PALAVILLERLAER.I YHR135C 2 2 7.6% 538 61715 9.0 U YCK1 SGDID:S000001177, Chr VIII from 374313-372697, reverse complement, Verified ORF, "Palmitoylated, plasma membrane-bound casein kinase I isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking; provides an essential function overlapping with that of Yck2p" * Jamie_Ashy_SPB_33.11860.11860.2 1.5585 0.0608 70.2 2576.3523 2579.0295 7378.9 151 3.588 20.8 1 K.KIGEGSFGVLFEGTNMINGVPVAIK.F Jamie_Ashy_SPB_49.17662.17662.2 1.7076 0.1017 17.7 1912.5521 1912.2573 6227.1 176 3.145 33.3 1 H.LIDFGMAKQYRDPKTK.Q Reverse_YGL111W 2 2 7.6% 463 51907 7.8 U NSA1 SGDID:S000003079, Chr VII from 299981-301372, Verified ORF, "Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis" * Jamie_Ashy_SPB_50.17916.17916.2 1.6083 0.1269 15.7 2421.9922 2419.656 8525.7 15 3.459 27.5 1 G.QLLYKGDHVHIYTSSGTIDDR.G * Jamie_Ashy_SPB_30.10584.10584.2 1.881 0.1184 17.3 1561.3322 1563.7576 6852.9 46 3.638 46.2 1 V.EKISGSDVCSVLLR.M Reverse_YPL057C 2 2 7.6% 382 44808 9.0 U SUR1 SGDID:S000005978, Chr XVI from 453054-451906, reverse complement, Verified ORF, "Probable catalytic subunit of a mannosylinositol phosphorylceramide (MIPC) synthase, forms a complex with probable regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p" * Jamie_Ashy_SPB_73.26016.26016.2 1.6111 0.0073 64.0 1574.3322 1575.8057 6971.3 12 2.633 42.3 1 R.SSKLLVINTNSDKR.L * Jamie_Ashy_SPB_13.04368.04368.2 2.3958 0.117 41.7 1548.4122 1548.7545 5741.0 15 3.819 50.0 1 V.SGMVDNSVGLPSTKR.L Reverse_YML119W 2 2 7.6% 357 40060 9.6 U YML119W SGDID:S000004588, Chr XIII from 30611-31684, Uncharacterized ORF, "Protein of unknown function, potential Cdc28p substrate" * Jamie_Ashy_SPB_54.19160.19160.2 1.7954 0.2009 44.6 1984.0322 1981.2133 7855.5 18 4.118 30.6 1 K.TPSNTLKPAVSTSSRTGYL.D * Jamie_Ashy_SPB_93.33492.33492.2 1.9408 0.0283 12.7 2162.3123 2163.438 6135.6 191 4.241 27.8 1 P.AVSTSSRTGYLDIPKTFYR.K Reverse_YDR451C 2 2 7.6% 353 39598 10.0 U YHP1 SGDID:S000002859, Chr IV from 1362171-1361110, reverse complement, Verified ORF, "One of two homeobox transcriptional repressors (see also Yox1p), that bind to Mcm1p and to early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval" * Jamie_Ashy_SPB_4.01225.01225.2 1.4627 0.197 21.9 2014.7522 2015.1095 3730.8 188 3.997 20.6 1 K.CPTIHHDEFISSTNAPST.K * Jamie_Ashy_SPB_6.02077.02077.1 1.4969 0.0115 79.3 1006.35 1004.9982 7996.0 89 2.28 56.2 1 K.CHSTNGSNK.H Reverse_YAL026C 4 4 7.5% 1355 153844 5.6 U DRS2 SGDID:S000000024, Chr I from 99698-95631, reverse complement, Verified ORF, "Integral membrane Ca(2+)-ATPase, potential aminophospholipid translocase required to form a specific class of secretory vesicles that accumulate upon actin cytoskeleton disruption; mutation affects maturation of the 18S rRNA" * Jamie_Ashy_SPB_29.10458.10458.4 2.6701 0.1281 17.5 3537.2166 3532.8694 6228.8 30 3.232 18.3 1 L.GNNDNFPNASADQLEGYKGRKQTTDYMRVIK.E * Jamie_Ashy_SPB_41.14719.14719.2 1.5277 0.1388 15.0 2550.8323 2550.8254 6033.8 4 3.883 28.3 1 K.IGAEQLTHITEPVGDQLKDEIATA.G * Jamie_Ashy_SPB_51.18279.18279.4 3.0836 0.076 10.9 3656.9766 3660.067 7746.2 9 3.689 18.8 1 R.TLTGTKDSFIYEIQGLEEVLSSTRVVTPTDTKE.Y * Jamie_Ashy_SPB_26.09049.09049.2 1.8893 0.0715 15.7 1399.4922 1399.6365 6035.7 33 3.238 45.8 1 E.VATRKIPTATANR.L YER111C 4 4 7.5% 1093 123806 8.8 U SWI4 SGDID:S000000913, Chr V from 385872-382591, reverse complement, Verified ORF, "DNA binding component of the SBF complex (Swi4p-Swi6p), a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair" * Jamie_Ashy_SPB_50.17748.17748.3 3.1385 0.1203 12.3 2104.5244 2107.1985 4835.9 4 3.646 35.3 1 R.RSQSISDEKEKAKDNENQ.V * Jamie_Ashy_SPB_31.10921.10921.4 2.2906 0.0291 42.3 4631.6963 4631.314 6017.6 51 2.847 14.2 1 K.DNENQVEKKKDPLNSVKTAMPSLESPSSLLPIQMSPLGKYSK.P * Jamie_Ashy_SPB_22.07852.07852.2 1.1139 0.0052 60.1 2662.632 2664.1475 8654.0 8 2.824 22.9 1 K.TAMPSLESPSSLLPIQMSPLGKYSK.P * Jamie_Ashy_SPB_76.27327.27327.4 2.9932 0.1687 10.2 2915.4165 2911.1057 5049.3 78 3.663 24.4 1 E.DEEPIPKSTSETSSPKNTKADAKFSNT.V YMR086W 3 3 7.5% 960 105874 9.7 U YMR086W SGDID:S000004692, Chr XIII from 439207-442089, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery" * Jamie_Ashy_SPB_96.34365.34365.2 1.2562 0.1778 12.9 2808.172 2807.1445 9175.9 106 3.953 20.5 1 S.GLQRQKSKTHQRISYDEAQRTFK.D * Jamie_Ashy_SPB_107.38282.38282.4 2.7434 0.0025 46.8 3654.7366 3657.0354 4604.6 41 2.865 20.2 1 K.VSQESLHSQPKKTSSLGNTSSTQAKKGQKAVQER.N * Jamie_Ashy_SPB_75.26941.26941.2 1.6995 0.1251 13.7 1464.7722 1464.5754 6237.7 127 3.154 39.3 1 S.GANTSSKKGNVGDTK.D Reverse_YML111W 2 2 7.5% 920 104786 8.0 U BUL2 SGDID:S000004579, Chr XIII from 46942-49704, Verified ORF, "Component of the Rsp5p E3-ubiquitin ligase complex, involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions, functional homolog of BUL1" * Jamie_Ashy_SPB_62.22263.22263.4 3.2667 0.139 11.7 5831.9365 5832.5483 10380.1 57 3.423 12.6 1 G.DLTGSIDTDHIDVNSIAENLELKKFIKNLAEFRDEILSELNKLQRLCTKE.N * Jamie_Ashy_SPB_46.16395.16395.2 1.8875 0.0339 76.5 2204.0923 2206.507 6283.7 254 3.495 25.0 1 K.LISQEEPREHPISATKTIR.I YJR036C 3 3 7.5% 892 103457 8.8 U HUL4 SGDID:S000003797, Chr X from 503305-500627, reverse complement, Verified ORF, "Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability" * Jamie_Ashy_SPB_17.05924.05924.3 3.0877 0.1374 14.5 2664.2344 2663.0647 6184.1 24 3.752 28.8 1 T.IRTCLIRDRKSKNVFETQQIR.A * Jamie_Ashy_SPB_34.12090.12090.4 2.228 0.0107 37.6 4461.2964 4460.145 7229.8 109 2.765 14.9 1 R.CIKEHQGDLLKSLRIEFVNEPGIDAGGLRKEWFFLLTK.S * Jamie_Ashy_SPB_29.10377.10377.2 1.5941 0.0846 14.2 1041.8121 1041.2382 2617.2 183 3.278 57.1 1 T.KFDFKSLR.S Reverse_YOR038C 2 2 7.5% 875 98445 8.3 U HIR2 SGDID:S000005564, Chr XV from 405387-402760, reverse complement, Verified ORF, "Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3, and H4 genes; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p" * Jamie_Ashy_SPB_36.12686.12686.4 2.1848 0.0843 59.4 2305.2166 2302.645 3556.4 420 3.04 25.9 1 R.AFRQLNKIRGKRNLEDNTK.C * Jamie_Ashy_SPB_67.23860.23860.4 1.9518 0.0633 41.2 5542.8564 5546.497 8581.9 37 3.009 13.4 1 K.DFTKHRFLLIKNENSLLVFLIGLNIDYKVDTITSPSKCEFLLKSNMK.E Reverse_YML042W 2 2 7.5% 670 77242 8.2 U CAT2 SGDID:S000004506, Chr XIII from 192788-194800, Verified ORF, "Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes" * Jamie_Ashy_SPB_77.27540.27540.3 2.3409 0.2225 10.0 2426.2444 2424.6812 5769.6 14 4.259 26.2 1 M.NKLGFFHRDVGNGNAANKLYTS.H * Jamie_Ashy_SPB_77.27420.27420.3 2.454 0.0215 43.4 3141.4744 3141.5945 6705.2 429 3.085 19.4 1 K.DLTSKLEPVPLSPLDQQKAFTIGQFNPR.K Reverse_YOR171C 2 2 7.5% 624 69639 6.4 U LCB4 SGDID:S000005697, Chr XV from 653884-652010, reverse complement, Verified ORF, "Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes" * Jamie_Ashy_SPB_24.08409.08409.2 1.6848 0.1183 16.0 1043.6522 1044.341 4436.6 6 3.799 68.8 1 D.LSLLIPTMR.T * Jamie_Ashy_SPB_55.19816.19816.4 3.287 0.1276 11.5 4241.1367 4245.5596 7491.1 2 3.2 17.1 1 K.LKFNDENLFNPSLEDKTSNNINNKSLLDPSSNPSPNPE.F YOR108W 2 2 7.5% 604 67200 6.8 U LEU9 SGDID:S000005634, Chr XV from 523027-524841, Verified ORF, "Alpha-isopropylmalate synthase II (2-isopropylmalate synthase), catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant" * Jamie_Ashy_SPB_117.42026.42026.2 1.4254 0.016 62.0 1989.5322 1991.3617 6291.2 2 2.864 43.8 1 K.NMLRDPSVKYRAFAPPK.M * Jamie_Ashy_SPB_41.14706.14706.3 2.5783 0.0097 43.8 2939.3643 2936.3835 8228.1 230 3.211 19.4 1 K.IPIPPRAPYGGELVVSAFSGSHQDAIKK.G Reverse_YER124C 2 2 7.5% 573 63999 6.1 U DSE1 SGDID:S000000926, Chr V from 409059-407338, reverse complement, Verified ORF, "Daughter cell-specific protein, may participate in pathways regulating cell wall metabolism; deletion affects cell separation after division and sensitivity to drugs targeted against the cell wall" * Jamie_Ashy_SPB_64.22806.22806.2 2.162 0.0612 14.9 1880.8322 1881.2323 11234.0 31 3.198 34.4 1 R.KIIKACGKYTSTAILNV.I * Jamie_Ashy_SPB_86.30903.30903.4 2.5323 0.0845 70.2 3112.1765 3108.7344 4743.8 361 3.103 20.7 1 K.YTSLTMMSQLIRKKDSRKKINIAPQR.F YFL001W 2 2 7.5% 442 50889 9.3 U DEG1 SGDID:S000001895, Chr VI from 147126-148454, Verified ORF, "Non-essential tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm" * Jamie_Ashy_SPB_26.09112.09112.4 3.9837 0.317 100.0 2406.4165 2409.7446 5466.3 206 5.246 26.7 1 G.TNAIVSKKDSIYANWSKEQLI.R * Jamie_Ashy_SPB_1.00326.00326.2 2.1443 0.0214 16.6 1407.2722 1408.6407 3736.3 6 3.208 59.1 1 K.QITNFKRTIISS.K YMR005W 2 2 7.5% 388 42336 9.7 U TAF4 SGDID:S000004607, Chr XIII from 276045-277211, Verified ORF, "TFIID subunit (48 kDa), involved in RNA polymerase II transcription initiation; potential Cdc28p substrate" * Jamie_Ashy_SPB_11.03722.03722.2 2.0707 0.0109 17.0 1101.4122 1100.3469 5545.5 55 2.813 62.5 1 A.LPKKDDKKK.N * Jamie_Ashy_SPB_38.13576.13576.2 1.8742 0.0031 69.1 2065.0522 2062.074 6704.7 53 2.794 31.6 1 K.DGKASNSSGQNAQQQSDPNK.M YER114C 3 3 7.4% 1040 115688 8.8 U BOI2 SGDID:S000000916, Chr V from 393708-390586, reverse complement, Verified ORF, "Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain" * Jamie_Ashy_SPB_49.17334.17334.4 3.0952 0.0876 16.2 3801.6165 3797.121 6434.8 305 3.128 15.7 1 K.PTTSSSTGFLNGDLENQATLINGIDTTKLNPVEAEF.W * Jamie_Ashy_SPB_7.02371.02371.4 2.1803 0.0194 40.6 2127.6165 2128.176 5909.4 248 2.665 30.4 1 K.RTSYIDQYSSSDSNFNSR.S * Jamie_Ashy_SPB_101.36338.36338.2 1.0223 0.0359 60.0 2612.2322 2610.8809 8095.9 81 2.855 20.5 1 K.EAEIFTETVKDDKNKRSASEAIK.G YJL187C 3 3 7.4% 819 92468 6.3 U SWE1 SGDID:S000003723, Chr X from 79261-76802, reverse complement, Verified ORF, "Protein kinase that regulates the G2/M transition by inhibition of Cdc28p kinase activity; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate" * Jamie_Ashy_SPB_16.05463.05463.3 2.4463 0.0338 61.1 1434.2344 1431.6322 4671.4 245 2.924 37.5 1 K.RSAEKTNSPISLK.Q * Jamie_Ashy_SPB_33.11571.11571.2 2.1304 0.0129 13.1 1618.6921 1619.0037 7321.3 56 2.953 41.7 1 N.KYNSLKRILLEIK.I * Jamie_Ashy_SPB_42.14937.14937.4 3.2137 0.0891 17.8 3802.2966 3807.0295 6503.5 152 3.303 17.2 1 R.SGDLSDAGRLSSTDIHSESLFSDITKVDTNDLFDF.E Reverse_YNL216W 2 2 7.4% 827 92413 5.0 U RAP1 SGDID:S000005160, Chr XIV from 241690-244173, Verified ORF, "DNA-binding protein involved in either activation or repression of transcription, depending on binding site context; also binds telomere sequences and plays a role in telomeric position effect (silencing) and telomere structure" * Jamie_Ashy_SPB_103.36774.36774.3 2.4243 0.1872 11.8 2618.4844 2622.9805 5677.4 140 3.875 25.0 1 F.LDRYFQKKVAIALTYDEDASFK.R * Jamie_Ashy_SPB_96.34603.34603.4 2.4938 0.0047 41.7 4598.5767 4597.4014 9493.1 1 3.227 17.5 1 R.KISPPLVKTKILNGDDDRVLKGFKDVEYVYELRKSLYVR.F Reverse_YBR142W 2 2 7.4% 773 87048 8.4 U MAK5 SGDID:S000000346, Chr II from 528311-530632, Verified ORF, "Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits" * Jamie_Ashy_SPB_76.27271.27271.2 1.5148 0.1007 13.4 2590.912 2588.0286 7916.7 1 3.562 34.1 1 K.KLTELANTKEHGIISNHGRKKVL.N * Jamie_Ashy_SPB_24.08506.08506.3 2.0513 0.0016 33.5 3815.6643 3814.062 8063.5 332 3.045 15.2 1 K.LLFTDSNSKSIDDEDSDVDIGLEDAAKKLWNDDK.R Reverse_YDL153C 2 2 7.4% 610 70260 4.7 U SAS10 SGDID:S000002312, Chr IV from 183019-181187, reverse complement, Verified ORF, "Component of the small (ribosomal) subunit (SSU) processosome required for pre-18S rRNa processing; essential nucleolar protein that, when overproduced, disrupts silencing" * Jamie_Ashy_SPB_97.34956.34956.3 2.3477 0.1189 75.3 3790.5842 3786.419 9039.2 89 4.214 16.4 1 K.EGEYVGSQGGSYVARVSKLKKQAKQYKKRKKVR.S * Jamie_Ashy_SPB_40.14293.14293.2 1.4847 0.0939 77.3 1306.8121 1308.5265 6024.5 11 3.158 50.0 1 R.AAIVANKHAEKR.S YNL106C 2 2 7.3% 1183 133331 8.6 U INP52 SGDID:S000005050, Chr XIV from 424497-420946, reverse complement, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in endocytosis; hyperosmotic stress causes translocation to actin patches" * Jamie_Ashy_SPB_37.13232.13232.4 2.9292 0.1078 19.5 5473.8564 5474.3433 8043.8 207 3.544 13.3 1 K.AELNGNGFRRLSNHEIYGFIGLIEIEGLMFIATITGKSKVAQPIPNKTVN.K * Jamie_Ashy_SPB_28.09828.09828.4 3.1912 0.246 78.6 4191.6567 4194.565 7290.6 30 3.963 17.1 1 K.RAGTRFNARGIDDDGHVANFVETEMIMYSSQYCYAF.T YAL019W 4 4 7.3% 1131 128507 5.4 U FUN30 SGDID:S000000017, Chr I from 114920-118315, Verified ORF, "Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p" * Jamie_Ashy_SPB_48.17204.17204.2 1.7772 0.0418 74.8 2439.2522 2438.653 5934.9 30 3.848 23.9 1 K.AVVEGFDETSAEPTPAPAPAPVER.E * Jamie_Ashy_SPB_32.11485.11485.4 3.2809 0.0893 16.6 3691.4165 3690.3938 7499.2 183 3.103 18.3 1 L.QNNLKELMSLLEFIMPNLFISKKESFDAIFK.Q * Jamie_Ashy_SPB_29.10266.10266.2 1.6142 0.1343 15.3 1273.7922 1273.4722 5335.9 392 3.55 45.0 1 G.ELPKDAKEKSK.L * Jamie_Ashy_SPB_84.30282.30282.2 1.8338 0.0713 12.4 2023.6721 2025.2688 8363.3 1 3.207 43.3 1 T.QVNDRQLLIDKFYEDK.D Reverse_YDL102W 2 2 7.3% 1097 124591 8.0 U CDC2 SGDID:S000002260, Chr IV from 276872-280165, Verified ORF, "Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)" * Jamie_Ashy_SPB_24.08462.08462.4 3.2882 0.1477 16.9 4942.8164 4942.5835 7531.4 32 4.038 14.3 1 M.GNAQKDGIIPAVISIIPNQLQNTLYYRSDVQINNELVFLPDEAR.N * Jamie_Ashy_SPB_70.25107.25107.4 2.8443 0.1646 11.6 4231.5767 4231.71 9052.5 259 3.159 15.7 1 K.WFPLKTDGSYGWISQKSVVEISDIAHDFTENLYHVF.K Reverse_YDR017C 3 3 7.3% 1050 119550 6.8 U KCS1 SGDID:S000002424, Chr IV from 482263-479111, reverse complement, Verified ORF, "Inositol hexaphosphate kinase, phosphorylates inositol hexakisphosphate (InsP6) to diphosphoinositol polyphosphates, required for proper vacuole morphology and involved in salt stress response, contains two leucine heptad repeats" * Jamie_Ashy_SPB_13.04464.04464.2 1.4179 0.1018 12.5 1095.3722 1096.1602 4957.9 197 3.177 55.6 1 H.NGMTTTNSNR.K * Jamie_Ashy_SPB_49.17347.17347.4 3.6613 0.141 10.8 4545.4165 4545.97 7470.0 264 3.949 15.4 1 F.TNPKEKISESHPVFQQPKKENSRNNVNEQRLSQSLTSYT.K * Jamie_Ashy_SPB_101.36067.36067.4 3.1027 0.0783 13.7 3071.2166 3069.2803 4947.8 72 3.584 22.2 1 Q.PQHSQQKAIDEGSGQGTETINNRHAVHK.N YLR450W 4 4 7.3% 1045 115692 7.6 U HMG2 SGDID:S000004442, Chr XII from 1032623-1035760, Verified ORF, "One of two isozymes of HMG-CoA reductase that convert HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks" * Jamie_Ashy_SPB_27.09700.09700.2 1.6798 0.1177 16.3 1091.7122 1090.2241 4483.2 5 3.976 62.5 1 D.VIYSVDHTR.Y * Jamie_Ashy_SPB_89.32082.32082.4 3.5296 0.0761 16.5 4060.4165 4060.6223 6901.0 84 4.099 16.7 1 V.SNIIYEAMFQEGAYLIRDYLFYISSFIGCAIYAR.H Jamie_Ashy_SPB_23.08164.08164.2 1.6906 0.0668 13.2 1467.7522 1465.6648 6213.5 302 3.436 35.7 1 A.GGGATTVLTKDGMTR.G * Jamie_Ashy_SPB_47.16749.16749.2 1.8763 0.0464 12.7 1991.6522 1991.201 7002.8 20 3.118 32.4 1 L.CSALAAGHLVQSHMTHNR.K Reverse_YGR150C 3 3 7.3% 864 101422 8.6 U YGR150C SGDID:S000003382, Chr VII from 793058-790464, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_10.03388.03388.2 2.0748 0.0117 12.8 1093.8522 1093.1387 5952.4 158 3.006 61.1 1 A.AENSSTLTNR.T * Jamie_Ashy_SPB_22.07790.07790.2 1.5817 0.0828 11.1 1792.2322 1792.0476 4459.3 20 3.102 35.7 1 G.KEYGSLVPLNRPDFR.A * Jamie_Ashy_SPB_56.20156.20156.4 2.9376 0.0645 75.3 4748.3765 4746.4004 8365.5 178 3.143 14.9 1 R.EKECIRLMTNYTCIDPFHKTSLFKMTDFTNWAQKTMEK.R Reverse_YKL188C 2 2 7.3% 853 97126 8.9 U PXA2 SGDID:S000001671, Chr XI from 88791-86230, reverse complement, Verified ORF, "Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter and ALD-related proteins" * Jamie_Ashy_SPB_20.06963.06963.3 2.34 0.0675 54.1 2936.1843 2935.3042 6994.5 388 3.27 20.8 1 K.STKPDEIKEFLNLNTESKRIINSEK.A * Jamie_Ashy_SPB_52.18414.18414.4 3.1597 0.1194 11.9 4115.1367 4115.768 7603.0 449 3.226 16.7 1 K.LQLTDESPKNKFFIPISCLVLGAAGWTYKIVFATAVD.Y YKR099W 3 3 7.3% 811 89604 8.2 U BAS1 SGDID:S000001807, Chr XI from 635489-637924, Verified ORF, "Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways" * Jamie_Ashy_SPB_7.02509.02509.2 1.9062 0.0585 85.7 1415.6721 1415.6353 4184.6 2 2.942 59.1 1 R.KRWTGSLDPNLK.K * Jamie_Ashy_SPB_13.04458.04458.4 3.2747 0.0811 18.2 4066.6165 4061.5417 4619.8 158 2.941 16.7 1 T.PNKEEQLQLSQQNNPSLIKQDILNVKENESSKLPR.L * Jamie_Ashy_SPB_81.29203.29203.2 1.7428 0.129 10.6 1493.8121 1496.5731 4771.1 284 3.713 45.5 1 L.RDEDGLDFWETL.R Reverse_YER001W 2 2 7.3% 762 88529 7.2 U MNN1 SGDID:S000000803, Chr V from 153519-155807, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" * Jamie_Ashy_SPB_33.11736.11736.2 1.5732 0.095 13.1 1347.8922 1347.5974 6123.9 34 3.048 50.0 1 G.QELVKGFPSTIK.P * Jamie_Ashy_SPB_34.12003.12003.4 3.341 0.2665 98.5 5581.6963 5579.404 8550.1 159 4.563 14.0 1 R.EKSMKDSKPFIEQINVPNAQMFCRDYARIREFAQFMDYKRRYLR.E Reverse_YLR266C 2 2 7.3% 701 81274 6.8 U PDR8 SGDID:S000004256, Chr XII from 677726-675621, reverse complement, Verified ORF, "Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon" * Jamie_Ashy_SPB_99.35404.35404.4 3.385 0.1901 51.3 3925.3364 3924.4937 6424.1 1 4.016 21.5 1 K.LVKWLNQFETQFKNQEFLSLTKWSTPGFVYRS.G * Jamie_Ashy_SPB_106.38090.38090.3 1.9464 0.0422 36.7 4300.7046 4301.765 6364.6 408 3.596 13.5 1 K.NQEFLSLTKWSTPGFVYRSGNDGLVSTRLSRLPNESSR.C YER054C 2 2 7.3% 548 62319 8.8 U GIP2 SGDID:S000000856, Chr V from 263697-262051, reverse complement, Verified ORF, "Putative regulatory subunit of the protein phosphatase Glc7p, involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p" * Jamie_Ashy_SPB_40.14224.14224.2 1.8414 0.1246 10.5 3009.112 3011.3152 6332.9 1 3.42 26.8 1 S.GLETTSIDDDLTTVAPKGFAHPAKISNPN.N * Jamie_Ashy_SPB_25.08770.08770.2 1.6401 0.0068 64.2 1727.0521 1728.9058 5932.2 163 3.031 30.0 1 K.ISNPNNGKGTNNTKLR.K Reverse_YBL019W 2 2 7.3% 520 59445 9.1 U APN2 SGDID:S000000115, Chr II from 184356-185918, Verified ORF, "Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII" * Jamie_Ashy_SPB_40.14275.14275.4 3.2148 0.1015 16.5 3492.4966 3492.0684 6165.2 10 3.41 20.7 1 L.IDAAKICRELKLSVLIFDIRSGYNSPRLNK.L * Jamie_Ashy_SPB_28.10041.10041.2 1.5581 0.0469 74.0 943.6122 944.1221 3777.9 358 3.721 57.1 1 K.KGIKDLNR.V Reverse_YJL014W 2 2 7.3% 534 58814 6.1 U CCT3 SGDID:S000003551, Chr X from 407473-409077, Verified ORF, "Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo" * Jamie_Ashy_SPB_30.10694.10694.2 1.7444 0.0251 69.3 1690.3922 1688.7971 6335.0 179 3.296 36.7 1 K.DGDIGTTFNGQAHKAR.L * Jamie_Ashy_SPB_42.14896.14896.2 1.4663 0.0278 63.5 2567.9722 2570.9119 6692.0 411 3.747 20.5 1 K.LDEVRNVITAGTVRAIRNNDSKK.V YER051W 2 2 7.3% 492 56530 6.8 U YER051W SGDID:S000000853, Chr V from 254655-256133, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_33.11703.11703.3 2.9646 0.0915 15.8 2796.1443 2796.0784 6081.5 32 3.976 23.9 1 L.TSSEVLSYPNSAKQIKSYRCPNHK.E * Jamie_Ashy_SPB_65.23218.23218.2 1.773 0.1308 19.6 1373.7722 1373.592 4050.3 33 3.852 50.0 1 F.LLFPPTQSNIDK.Y Reverse_YKL172W 2 2 7.3% 427 49734 6.4 U EBP2 SGDID:S000001655, Chr XI from 125764-127047, Verified ORF, "Essential protein required for the maturation of 25S rRNA and 60S ribosomal subunit assembly, localizes to the nucleolus; constituent of 66S pre-ribosomal particles" * Jamie_Ashy_SPB_14.04816.04816.3 2.0467 0.0405 49.4 1300.4644 1302.4734 3635.9 353 2.91 38.9 1 K.ELTERKELQR.K * Jamie_Ashy_SPB_3.00777.00777.4 2.5304 0.0272 50.5 2460.8167 2465.8552 5040.9 11 3.438 30.8 1 R.KLEDRAVLVADLSQKYFALER.E Reverse_YGR281W 4 4 7.2% 1477 166727 7.7 U YOR1 SGDID:S000003513, Chr VII from 1052830-1057263, Verified ORF, "Plasma membrane transporter of the ATP-binding cassette (ABC) family, mediates export of many different organic anions including oligomycin" * Jamie_Ashy_SPB_21.07344.07344.4 2.7481 0.1081 13.0 3776.0166 3778.5198 7415.5 8 3.806 18.2 1 T.GRFLVPDQPIIALKRRLDFLGLQSIDVNDILIK.G * Jamie_Ashy_SPB_35.12354.12354.3 2.3555 0.1682 18.3 3248.3643 3248.5493 8588.9 315 3.879 16.7 1 S.ISNVAREEKSSTHGDAVIKGSNATQSMETAK.K * Jamie_Ashy_SPB_31.10956.10956.4 3.5232 0.2233 78.0 3017.4165 3014.424 6883.4 8 4.53 20.2 1 I.RAKQGGSLTIGREGIETMDGAPLIDLDAK.L * Jamie_Ashy_SPB_25.08778.08778.2 1.6973 0.0784 12.9 1534.5721 1534.7496 5113.9 456 3.251 41.7 1 R.KQPFIDSETVKEI.D Reverse_YDR060W 4 4 7.2% 1025 116676 5.0 U MAK21 SGDID:S000002467, Chr IV from 570645-573722, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein" * Jamie_Ashy_SPB_38.13428.13428.4 3.0577 0.093 18.8 2929.2966 2932.3684 6624.5 259 3.415 21.7 1 K.LMNKRQKKKARKASVEKNEEEEKE.E * Jamie_Ashy_SPB_34.12253.12253.4 3.1316 0.0208 66.6 3243.2566 3249.247 7903.9 246 2.695 20.4 1 K.ASVEKNEEEEKEEESSEDEESSEAFSRK.K * Jamie_Ashy_SPB_14.04988.04988.4 2.575 0.1408 12.0 1710.6565 1714.9147 3496.9 402 3.725 29.2 1 K.KTTFYQYFFKDEP.K * Jamie_Ashy_SPB_112.40011.40011.3 2.8993 0.1012 84.3 2322.0244 2321.5872 6524.6 1 3.236 33.8 1 K.DSSIITTQTILGGDKEEKKEK.E YIR002C 3 3 7.2% 993 114058 7.3 U MPH1 SGDID:S000001441, Chr IX from 360393-357412, reverse complement, Verified ORF, "Member of the DEAH family of helicases, functions in an error-free DNA damage bypass pathway that involves homologous recombination, mutations confer a mutator phenotype" * Jamie_Ashy_SPB_45.15949.15949.4 3.4968 0.0775 16.2 4328.4966 4324.9214 6688.6 6 3.413 15.8 1 N.VVKFIDRFNSSYRLLALTATPASDLEGVQEVVNNLDISK.I * Jamie_Ashy_SPB_59.21216.21216.3 1.9878 0.2166 14.7 1793.7544 1795.8083 6035.7 253 4.08 29.7 1 K.ALDNLENDSTEEASSSL.E * Jamie_Ashy_SPB_36.12741.12741.3 2.3008 0.1646 15.4 1768.4343 1772.0642 4682.5 24 3.709 37.5 1 D.CGLPTKIEKNVKDIR.G YBL106C 3 3 7.2% 1010 111678 8.8 U SRO77 SGDID:S000000202, Chr II from 13879-10847, reverse complement, Verified ORF, "Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Sro7p and Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p" * Jamie_Ashy_SPB_82.29473.29473.4 3.424 0.0988 12.9 4459.0967 4457.2373 5745.9 291 4.201 15.3 1 R.NQMSKLKIENFQKSVFLPKERLSPVISIQWNPRDIGTI.L * Jamie_Ashy_SPB_21.07524.07524.2 1.593 0.1218 17.2 2036.8121 2039.4156 8964.1 268 4.008 27.8 1 G.MMTIAQSESFLKGGIPATR.N * Jamie_Ashy_SPB_38.13561.13561.2 1.7295 0.1182 16.0 1882.2922 1880.151 6188.0 418 3.282 26.7 1 H.TPIDTLYNPDLKIGYR.P YPL212C 2 2 7.2% 544 62143 8.1 U PUS1 SGDID:S000006133, Chr XVI from 153148-151514, reverse complement, Verified ORF, "tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA" * Jamie_Ashy_SPB_13.04513.04513.2 1.0103 0.0314 60.0 799.7922 797.9357 3154.2 282 2.578 66.7 1 K.RPKSGPR.L * Jamie_Ashy_SPB_20.07084.07084.4 3.4526 0.0891 15.4 3450.0566 3449.8694 4164.9 80 3.442 18.3 1 K.AFVEAGAISKDNSNDLKKNGFMRAARTDKGVH.A Reverse_YJR051W 2 2 7.2% 501 55065 9.0 U OSM1 SGDID:S000003812, Chr X from 529775-531280, Verified ORF, "Fumarate reductase, catalyzes the reduction of fumarate to succinate, required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity" Jamie_Ashy_SPB_4.01303.01303.2 1.9738 0.0318 14.5 1092.9722 1092.2035 4133.6 3 3.187 72.2 1 V.CELLSSGGLR.N * Jamie_Ashy_SPB_60.21302.21302.4 2.9428 0.1363 12.2 2839.6165 2836.2693 5825.4 7 3.775 25.3 1 R.RGTTPHLLIGGLGRLAEAALFKWNNE.R Reverse_YLR303W 2 2 7.2% 444 48672 6.4 U MET17 SGDID:S000004294, Chr XII from 732544-733878, Verified ORF, "O-acetyl homoserine-O-acetyl serine sulfhydrylase, required for sulfur amino acid synthesis" * Jamie_Ashy_SPB_12.04116.04116.2 1.9357 0.1262 22.0 1744.5721 1745.0311 5196.4 7 3.99 34.4 1 K.VGFSLVGGFGNSLYKKA.N * Jamie_Ashy_SPB_93.33514.33514.2 1.6571 0.0739 13.3 1919.8322 1921.0818 8223.6 35 3.515 31.2 1 K.KANEHHSHSALGPYSVW.S YOR098C 2 2 7.1% 1076 113581 9.6 U NUP1 SGDID:S000005624, Chr XV from 511178-507948, reverse complement, Verified ORF, "Nuclear pore complex (NPC) subunit, involved in protein import/export and in export of RNAs, possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; potential Cdc28p substrate" * Jamie_Ashy_SPB_44.15788.15788.4 3.4888 0.0516 15.8 4646.1367 4651.189 9108.1 7 4.107 15.9 1 K.KPSFTFASSKTSQPKPLFSFGKSDAAKEPPGSNTSFSFTKPPAN.E * Jamie_Ashy_SPB_46.16378.16378.4 3.6341 0.1112 11.5 3136.5764 3140.35 5602.2 133 3.186 19.4 1 N.TATNALRPSDIFGANAASGSNSNVTNPSSIFG.G YMR266W 2 2 7.1% 953 107672 8.6 U YMR266W SGDID:S000004879, Chr XIII from 798517-801378, Uncharacterized ORF, "Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant" * Jamie_Ashy_SPB_39.13764.13764.4 3.0462 0.0776 10.9 4060.9766 4057.753 6399.1 165 3.444 15.8 1 K.EPTKAMDLLASNLPKASNFFMSYVILQGLSISSGALLQ.I * Jamie_Ashy_SPB_70.25172.25172.4 2.7114 0.0257 52.5 3277.3765 3281.7888 7772.2 299 2.523 19.0 1 K.VIPVDTMKPLDGVSDTPSFKNIYKGIESTK.V Reverse_YLR240W 2 2 7.1% 875 100921 7.8 U VPS34 SGDID:S000004230, Chr XII from 617535-620162, Verified ORF, "Phosphatidylinositol 3-kinase responsible for the synthesis of phosphatidylinositol 3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; similar to p110 subunit of mammalian PI 3-kinase" * Jamie_Ashy_SPB_42.15117.15117.2 1.7018 0.1009 17.6 1791.9922 1791.008 7373.0 28 3.5 34.4 1 K.SLISALTDNPIFEIAGE.Q * Jamie_Ashy_SPB_17.05952.05952.4 2.5799 0.14 19.2 5136.5767 5130.9985 7139.5 6 3.454 14.8 1 P.TYIPLTLNRNREKDFVQLSVILDSSPFVNSGILMLEPNLIKQSPK.L Reverse_YDL056W 3 3 7.1% 833 93908 9.3 U MBP1 SGDID:S000002214, Chr IV from 352877-355378, Verified ORF, "Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes" * Jamie_Ashy_SPB_22.07807.07807.3 2.7501 0.0999 14.4 2344.8542 2346.6836 4931.5 43 3.73 31.2 1 E.GNEDKSSEKLVELTKLSVQIK.T * Jamie_Ashy_SPB_79.28390.28390.4 3.1282 0.0842 15.9 3052.4966 3052.2468 5172.4 5 3.42 23.3 1 K.FQASNNQQPQQQRSDHREEELIGLTP.S * Jamie_Ashy_SPB_20.07185.07185.2 1.4169 0.1984 10.6 2410.612 2412.7637 4476.5 47 4.146 26.2 1 H.REEELIGLTPSQPGMTSRISPL.Q Reverse_YGL009C 3 3 7.1% 779 85794 5.9 U LEU1 SGDID:S000002977, Chr VII from 478657-476318, reverse complement, Verified ORF, "Isopropylmalate isomerase, catalyzes the second step in the leucine biosynthesis pathway" * Jamie_Ashy_SPB_10.03510.03510.2 1.6453 0.0046 66.0 1203.3922 1202.4825 5021.5 31 2.913 66.7 1 R.KPVNDFKLLK.S * Jamie_Ashy_SPB_77.27571.27571.4 2.7239 0.0022 45.6 3034.6965 3035.4802 4301.6 67 2.844 22.8 1 R.VVAAASRLDEIRGNTCSGIFVKDVKISK.L * Jamie_Ashy_SPB_90.32096.32096.2 1.6581 0.0785 12.9 1957.4922 1958.1375 6214.6 195 3.482 31.2 1 R.DIYLLFSGNEDQHVVHA.D YPR141C 2 2 7.1% 729 84004 8.7 U KAR3 SGDID:S000006345, Chr XVI from 817919-815730, reverse complement, Verified ORF, "Minus-end-directed microtubule motor that functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate" * Jamie_Ashy_SPB_86.30945.30945.4 2.6701 0.0659 67.5 4929.2563 4934.769 5705.2 110 3.076 14.6 1 R.TQAILFEKKATLDLLKDELTETKEKINAVNLKFETLREEKIK.I * Jamie_Ashy_SPB_7.02316.02316.2 1.7748 0.0419 11.6 1229.2122 1230.4471 5418.3 59 2.764 55.6 1 N.EILIKEETVR.R YMR216C 2 2 7.1% 742 83238 6.2 U SKY1 SGDID:S000004829, Chr XIII from 701038-698810, reverse complement, Verified ORF, "SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1" * Jamie_Ashy_SPB_65.23138.23138.2 1.2464 0.1709 13.4 2826.5122 2829.3765 10224.4 246 3.735 20.0 1 F.NHKGPNGVHVVMVFEVLGENLLALIK.K * Jamie_Ashy_SPB_89.31994.31994.4 3.6748 0.1095 11.6 3104.0964 3106.234 5024.9 68 3.932 21.8 1 S.DEKWAECQTSMPCGSSSNSKSRSIEKD.L YGL022W 2 2 7.1% 718 81529 8.0 U STT3 SGDID:S000002990, Chr VII from 452409-454565, Verified ORF, "Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; forms a subcomplex with Ost3p and Ost4p and is directly involved in catalysis" * Jamie_Ashy_SPB_83.29804.29804.2 1.667 0.1514 10.6 2045.7922 2047.3337 6984.8 45 3.331 30.6 1 N.NTHIAIVGKAMASPEEKSY.E * Jamie_Ashy_SPB_83.29794.29794.4 2.4522 0.0448 50.7 3716.4165 3722.243 8137.0 60 3.073 18.3 1 R.IYQLKKDDAQGRTLRDVGELTRSSTKTRRSIK.R contaminant_A46538 2 2 7.1% 580 64493 5.7 U owl|A46538| immunoglobulin heavy chain, secreted form - Atlantic cod * Jamie_Ashy_SPB_7.02484.02484.4 3.1519 0.0874 18.3 2969.6965 2967.3135 6915.2 16 2.863 21.5 1 E.WISHIWDNGDIYKNNALSSKFTISR.D * Jamie_Ashy_SPB_113.40684.40684.2 1.49 0.1235 12.4 1930.3722 1928.2567 6867.0 7 3.83 36.7 1 Y.IARTKITYDEWSKGMK.W Reverse_YCL037C 2 2 7.1% 434 48060 8.9 U SRO9 SGDID:S000000542, Chr III from 58678-57374, reverse complement, Verified ORF, "Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif" * Jamie_Ashy_SPB_102.36546.36546.2 1.2707 0.0536 62.6 2226.7922 2224.4314 9557.2 42 2.837 29.4 1 K.EINIETEFTEPLWNSWSK.S * Jamie_Ashy_SPB_15.05098.05098.2 1.899 0.0331 13.0 1292.9922 1292.4783 6232.6 15 2.931 62.5 1 S.GSRKTGSVTISAK.I YKR021W 3 3 7.0% 915 102540 6.0 U YKR021W SGDID:S000001729, Chr XI from 478877-481624, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * Jamie_Ashy_SPB_68.24418.24418.2 1.6151 0.0891 11.6 1783.9922 1784.9354 6570.5 48 3.488 39.3 1 S.SNRPSMSDEDYLLEK.S * Jamie_Ashy_SPB_28.09822.09822.3 3.3016 0.1725 38.9 2518.3743 2520.7957 4863.8 25 4.433 27.3 1 S.DEDYLLEKSGASVYIPPTAEPPK.D * Jamie_Ashy_SPB_32.11329.11329.4 2.8039 0.1168 18.3 3596.0166 3593.937 8062.7 228 3.091 16.2 1 R.RGSVPANFHGNSLSPHTFISDLFTKTFSNSGATP.S YNL085W 3 3 7.0% 830 94494 5.6 U MKT1 SGDID:S000005029, Chr XIV from 467133-469625, Verified ORF, "Protein that forms a complex with Pbp1p that may mediate posttranscriptional regulation of HO endonuclease; involved in propagation of M2 dsRNA satellite of L-A virus" * Jamie_Ashy_SPB_26.09267.09267.4 2.1728 0.1793 17.1 5088.4165 5092.5557 6991.0 36 3.702 14.7 1 A.TTRSNTESVLLQRSRGWTQWNNLISSNQNSYIDQPIQPQEPFR.H * Jamie_Ashy_SPB_7.02280.02280.2 1.2736 0.0090 61.2 984.8722 986.1564 6319.8 46 2.463 64.3 1 K.EKLLEPTR.W * Jamie_Ashy_SPB_98.35116.35116.2 1.9214 0.0789 13.1 1474.8322 1475.7306 4943.7 2 3.699 59.1 1 L.LEPTRWGEVFLK.L YER001W 2 2 7.0% 762 88529 7.2 U MNN1 SGDID:S000000803, Chr V from 153519-155807, Verified ORF, "Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family" * Jamie_Ashy_SPB_13.04458.04458.3 2.8661 0.1035 15.3 3050.2144 3052.494 4619.8 255 3.407 20.8 1 L.LNLLQEFVFLDIDAISYEKIDNYFK.T * Jamie_Ashy_SPB_56.19902.19902.4 3.7111 0.0888 12.4 3447.6165 3446.9363 8525.6 198 3.389 19.8 1 K.DQFKTFEEMEKYYYITPISSKYVILPDP.K contaminant_PIGR_RAT 4 4 7.0% 769 84798 5.2 U owl|P15083| POLYMERIC-IMMUNOGLOBULIN RECEPTOR PRECURSOR (PLGR) (CONTAINS:... * Jamie_Ashy_SPB_31.11089.11089.2 1.8083 0.1847 97.8 1397.1921 1395.6628 5586.4 2 4.038 59.1 1 K.DRAILFMKGTSR.D * Jamie_Ashy_SPB_13.04675.04675.3 3.0779 0.0719 15.3 2246.4844 2245.5347 5704.8 1 3.693 33.8 1 K.KPDLEVTPQNATAVIGETFTI.S * Jamie_Ashy_SPB_44.15666.15666.2 1.9317 0.1207 22.3 2171.2922 2170.359 6446.3 72 3.445 27.8 1 Q.NATAVIGETFTISCHYPCK.F * Jamie_Ashy_SPB_88.31528.31528.2 1.5531 0.0755 10.2 1495.1522 1492.6346 3981.2 495 3.343 38.5 1 G.SPHINPTDANARAK.D YPR179C 2 2 7.0% 655 75463 6.3 U HDA3 SGDID:S000006383, Chr XVI from 895760-893793, reverse complement, Verified ORF, "Subunit of a possibly tetrameric trichostatin A-sensitive class II histone deacetylase complex that contains an Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for the activity of the complex; has similarity to Hda2p" * Jamie_Ashy_SPB_9.03261.03261.3 2.3095 0.1462 15.7 1408.3744 1409.6316 5359.8 318 3.483 43.2 1 L.RDRLGTLPPDLR.P * Jamie_Ashy_SPB_68.24244.24244.3 2.348 0.155 15.6 3768.7444 3765.0132 6317.1 19 4.15 16.7 1 K.NDYYTNPLKQDMTQLTGITTADNSSNVNYHLSSG.I YLR299W 2 2 7.0% 660 73181 5.9 U ECM38 SGDID:S000004290, Chr XII from 726071-728053, Verified ORF, "Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; expression induced mainly by nitrogen starvation" * Jamie_Ashy_SPB_100.35918.35918.2 1.5606 0.0543 68.9 2092.0122 2091.5627 7715.1 5 3.719 34.2 1 K.IGGLAVGVPGELMGLYRLFK.E * Jamie_Ashy_SPB_28.09996.09996.3 2.6575 0.1981 10.6 2856.8643 2857.0244 7651.2 343 4.124 21.0 1 W.TLYDPAYDINNPHGTAHFSIVDSHGN.A Reverse_contaminant_A46507 2 2 7.0% 585 61487 4.7 U owl|A46507| immunoglobulin alpha heavy chain - chicken * Jamie_Ashy_SPB_18.06284.06284.2 1.5924 0.0248 64.0 2406.1921 2408.6697 5201.8 238 2.806 26.3 1 R.QWDEVSVKLKSYLSYFDGDK.E * Jamie_Ashy_SPB_48.17313.17313.2 1.5729 0.0809 10.5 2310.872 2309.7263 6924.0 400 3.267 27.5 1 L.WQIAAGASPLGEVLCVLLVRR.E Reverse_YEL061C 3 3 6.9% 1000 113311 8.1 U CIN8 SGDID:S000000787, Chr V from 39537-36535, reverse complement, Verified ORF, "Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation" * Jamie_Ashy_SPB_104.37447.37447.2 1.4653 0.1291 12.2 2623.4922 2624.8674 8745.6 76 4.151 26.1 1 K.NEDEIAHKKPSIVSQVSNSNINSL.R * Jamie_Ashy_SPB_14.04982.04982.2 1.9325 0.1315 24.1 1607.6522 1604.9458 4690.3 13 3.329 42.9 1 D.IVASTATTLNQMILK.K * Jamie_Ashy_SPB_69.24876.24876.2 1.1809 0.1687 12.9 3374.8523 3373.7944 8782.5 5 3.593 17.2 1 M.IFSGLQPKNKINKAKSAYELTSCTEESTVK.A YDR103W 3 3 6.9% 917 102727 5.5 U STE5 SGDID:S000002510, Chr IV from 658345-661098, Verified ORF, "Scaffold protein that, in response to pheromone, shuttles from the nucleus to the plasma membrane and assembles kinases Ste11p, Ste7p, and Fus3p into a specific signaling complex; active oligomeric form interacts with Ste4p-Ste18p complex" * Jamie_Ashy_SPB_84.30200.30200.2 1.9703 0.1645 34.6 1797.9722 1797.1034 6466.9 157 4.115 36.7 1 R.FPPYSPLLPPFGLSYT.P * Jamie_Ashy_SPB_5.01834.01834.2 1.9934 0.0773 10.5 1125.2322 1124.2853 6323.8 97 3.237 55.6 1 I.LAAPPKERNQ.I * Jamie_Ashy_SPB_73.26130.26130.3 1.9599 0.0308 36.0 4486.7944 4489.189 8172.9 28 2.783 15.3 1 R.SYFIQILLNNFQEELQDWRIDGDYGLLRLVDKLMISK.D Reverse_YMR089C 3 3 6.9% 825 93276 7.6 U YTA12 SGDID:S000004695, Chr XIII from 448085-445608, reverse complement, Verified ORF, "Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes" * Jamie_Ashy_SPB_54.19435.19435.4 2.6745 0.0312 53.7 2885.4565 2886.289 5592.7 309 2.561 24.3 1 K.LYWGCVAHGAEHYAVVKKEEPSLLK.S * Jamie_Ashy_SPB_6.01998.01998.2 2.127 0.0265 17.6 1390.1122 1390.5358 4605.1 1 3.52 68.2 1 I.EDIFVISPANER.A * Jamie_Ashy_SPB_15.05136.05136.4 3.1446 0.0985 14.6 1968.0966 1964.1947 5048.1 9 3.684 27.2 1 A.KSRSLGFIGGRSGGAAQASR.R YOR233W 2 2 6.9% 800 90088 9.5 U KIN4 SGDID:S000005759, Chr XV from 775846-778248, Verified ORF, "Nonessential protein kinase with unknown cellular role" * Jamie_Ashy_SPB_53.18939.18939.3 2.477 0.1402 15.3 4468.4644 4468.9277 6065.8 16 4.068 14.9 1 R.GNKHTSVAGLVTIPGSPTTARTRNAPSSKLTEHVKDSSQTSFT.Q * Jamie_Ashy_SPB_77.27759.27759.2 1.8518 0.0542 11.7 1297.0122 1295.3951 3694.6 5 3.367 54.5 1 N.GSRSTVESRTSK.G Reverse_YOR267C 2 2 6.9% 759 85696 7.3 U HRK1 SGDID:S000005793, Chr XV from 824864-822585, reverse complement, Verified ORF, "Protein kinase implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis" * Jamie_Ashy_SPB_34.11962.11962.4 3.284 0.1322 13.9 3008.8965 3013.118 4988.1 3 3.234 22.2 1 T.SISTANSHSHTNNNLNTTTYNTTTTTSK.M * Jamie_Ashy_SPB_49.17523.17523.2 1.2708 0.0305 61.6 2765.2322 2763.0884 5899.2 1 3.144 30.4 1 K.RGIFNLFSKSSRILQPPNNSSNER.D Reverse_YKR024C 2 2 6.9% 742 83308 9.3 U DBP7 SGDID:S000001732, Chr XI from 487015-484787, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis" * Jamie_Ashy_SPB_99.35454.35454.4 2.6879 0.0694 69.9 3659.5366 3665.0593 6135.3 18 2.943 20.6 1 K.VALNKFASDGVVRREINLHWTTANTDWEYEK.D * Jamie_Ashy_SPB_28.09808.09808.4 2.4809 0.1275 81.2 2080.7766 2086.2693 3009.9 74 3.501 31.6 1 K.ISNEGRGPIIGEDGTTNAKR.K Reverse_YKR018C 2 2 6.9% 725 81754 5.2 U YKR018C SGDID:S000001726, Chr XI from 475543-473366, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus" * Jamie_Ashy_SPB_39.13944.13944.3 2.2653 0.0423 48.3 2180.3643 2177.5825 7156.6 15 3.207 31.9 1 R.MRIKSVDISDMLAVAEELR.G * Jamie_Ashy_SPB_67.23976.23976.2 1.2346 0.1303 71.3 3402.172 3401.6619 9928.7 24 3.634 16.7 1 K.DKQEEPTLEYPIDVSNFADGGTIFTAPTSEK.D YBR172C 2 2 6.9% 740 81397 8.8 U SMY2 SGDID:S000000376, Chr II from 581367-579145, reverse complement, Verified ORF, "Protein of unknown function that interacts with Myo2p; has similarity to S. pombe Mpd2" * Jamie_Ashy_SPB_46.16329.16329.4 3.4889 0.1017 11.8 4034.3765 4033.5176 7248.2 348 3.058 16.2 1 K.ELIQETIYANSDVMDGRRFATEFIKRRVACEKQG.D * Jamie_Ashy_SPB_65.23372.23372.3 2.6819 0.1355 18.4 2919.0544 2918.0723 6769.3 156 4.301 20.0 1 K.RRVACEKQGDDPLSWNEALALSGNDD.D Reverse_YLR035C 2 2 6.9% 695 78248 8.1 U MLH2 SGDID:S000004025, Chr XII from 214457-212370, reverse complement, Verified ORF, "Protein required for DNA mismatch repair in mitosis and meiosis; involved in the repair of mutational intermediates; forms a complex with Pms1p and Msh1p to repair mismatched DNA" * Jamie_Ashy_SPB_100.35697.35697.4 2.6458 0.1428 17.0 2846.0964 2850.2598 4894.3 81 3.337 23.6 1 K.ENISPQVHKDGLMNTEIGIREELLL.K * Jamie_Ashy_SPB_105.37723.37723.4 2.6221 0.1209 15.1 2771.3765 2775.198 4369.0 209 3.425 25.0 1 L.TEQMQKREIAGNKQLSVLSFYFR.I YKL048C 2 2 6.9% 640 72150 8.1 U ELM1 SGDID:S000001531, Chr XI from 348780-346858, reverse complement, Verified ORF, "Serine/threonine protein kinase that regulates cellular morphogenesis, septin behavior, and cytokinesis; required for the regulation of other kinases; forms part of the bud neck ring" * Jamie_Ashy_SPB_95.34232.34232.3 1.7164 0.2247 11.0 4923.4146 4927.5596 6096.0 127 4.252 8.7 1 S.NESLPNLTVNNDKQNSDMKTDRSESSSHSSLKIPTPIKAMIRLK.S * Jamie_Ashy_SPB_106.37987.37987.2 1.704 0.1157 88.3 2173.3123 2170.5322 4963.3 89 3.452 28.9 1 R.SESSSHSSLKIPTPIKAMIR.L Reverse_YJR002W 2 2 6.9% 593 66953 4.7 U MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p" * Jamie_Ashy_SPB_89.32004.32004.2 1.7273 0.0516 10.5 1428.2522 1426.6555 5876.1 88 2.918 45.8 1 R.IELSTSAPKPVFH.V * Jamie_Ashy_SPB_62.22106.22106.4 2.985 0.1446 12.2 3296.5764 3297.5288 7467.8 4 3.687 22.8 1 D.LKVKDMASDHEEEDLEEDEKPDKVDSHK.K YJR002W 2 2 6.9% 593 66953 4.7 U MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p" * Jamie_Ashy_SPB_40.14257.14257.4 2.7528 0.0105 49.5 3044.5366 3042.3796 5863.1 345 2.485 19.1 1 K.DPSATSKDVKAYIDSVINTCKNGSITKK.A * Jamie_Ashy_SPB_79.28380.28380.2 1.8501 0.1198 18.1 1464.8322 1464.6337 4255.3 28 3.91 50.0 1 K.NGVAMSKEELTRE.D YMR217W 2 2 6.9% 525 58482 6.5 U GUA1 SGDID:S000004830, Chr XIII from 701789-703366, Verified ORF, "GMP synthase, an enzyme that catalyzes the second step in the biosynthesis of GMP from inosine 5'-phosphate (IMP); transcription is not subject to regulation by guanine but is negatively regulated by nutrient starvation" * Jamie_Ashy_SPB_23.08056.08056.2 1.4003 0.02 61.6 2323.3523 2322.7158 6485.6 15 2.691 30.0 1 R.FHAILVDNGVLRLNEAANVKK.T * Jamie_Ashy_SPB_73.26299.26299.2 1.5826 0.0575 70.5 1857.8322 1859.2278 6644.8 114 2.961 35.7 1 K.KRKIIGNTFIHVFER.E YNL199C 2 2 6.9% 534 58062 9.0 U GCR2 SGDID:S000005143, Chr XIV from 266531-264927, reverse complement, Verified ORF, "Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p" * Jamie_Ashy_SPB_74.26467.26467.2 1.5878 0.1548 17.1 2697.372 2697.9187 6446.5 13 3.939 25.0 1 Y.NATLSSGSIDDRSTSPKSAIELYQR.F * Jamie_Ashy_SPB_36.12849.12849.2 2.4429 0.0082 13.4 1513.1921 1513.7919 7399.3 24 3.46 54.5 1 R.FQQMIKELELSF.D Reverse_YOR227W 3 3 6.8% 1246 139457 8.9 U YOR227W SGDID:S000005753, Chr XV from 762825-766565, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_2.00585.00585.4 2.1995 0.0043 39.1 1822.3365 1822.9207 5925.6 409 2.82 31.1 1 K.KEDKSKDPEDTSDSIK.S * Jamie_Ashy_SPB_16.05508.05508.4 3.0268 0.2158 96.4 3847.8965 3845.129 7474.8 33 4.117 18.5 1 K.DIHLKSARADLSSSEQDTASDAASNTAGTLPSTKLQR.A * Jamie_Ashy_SPB_78.28052.28052.2 1.382 0.2244 24.0 3305.0322 3306.7441 6695.5 42 4.619 16.1 1 S.LENNVNQGSLPRQPSAITKTPVAATSPLTATK.K Reverse_YJL029C 2 2 6.8% 822 95449 5.6 U VPS53 SGDID:S000003566, Chr X from 390769-388301, reverse complement, Verified ORF, "Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; required for vacuolar protein sorting" * Jamie_Ashy_SPB_82.29532.29532.4 3.7758 0.2989 94.4 3571.4966 3574.033 7357.1 125 5.432 19.0 1 V.KDLFNWKYVDRNFQSLIFALTSPQEQQQK.I * Jamie_Ashy_SPB_65.23298.23298.4 2.7921 0.0195 53.5 3241.6165 3236.6262 7340.7 122 2.788 21.8 1 K.LVSKLTVMYRSYDEIAANSWDNNIFER.W YKL134C 2 2 6.8% 770 87997 7.6 U OCT1 SGDID:S000001617, Chr XI from 191441-189129, reverse complement, Verified ORF, "Mitochondrial intermediate peptidase, cleaves N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis" * Jamie_Ashy_SPB_24.08463.08463.2 1.791 0.0443 10.9 1942.6522 1941.1509 7387.5 101 3.8 31.2 1 N.INGQDANNSKISQYLFK.K * Jamie_Ashy_SPB_38.13638.13638.4 2.3837 0.0206 42.4 4280.2563 4283.837 4459.0 261 2.935 14.7 1 K.MRNDFSESGKLTYIMNLDRLSDTLCRVIDLCEFIR.S Reverse_YGL244W 2 2 6.8% 558 65869 5.4 U RTF1 SGDID:S000003213, Chr VII from 41498-43174, Verified ORF, "Subunit of the RNA polymerase II-associated Paf1 complex; directly or indirectly regulates DNA-binding properties of Spt15p (TATA box-binding protein) and relative activities of different TATA elements" * Jamie_Ashy_SPB_12.04192.04192.2 1.8354 0.0778 14.6 1839.2122 1839.0646 5252.2 33 3.374 36.7 1 M.RYSTKGTRKDTGVNVR.G * Jamie_Ashy_SPB_105.37796.37796.3 2.6239 0.1095 11.2 2868.5344 2867.7363 5244.5 110 3.751 25.0 1 D.DKYDEERYDEEDDEDENDSYHR.N YPL091W 2 2 6.8% 483 53441 7.8 U GLR1 SGDID:S000006012, Chr XVI from 375499-376950, Verified ORF, "Cytosolic and mitochondrial glutathione oxidoreductase, converts oxidized glutathione to reduced glutathione" * Jamie_Ashy_SPB_5.01760.01760.2 2.2223 0.0722 18.4 1479.2322 1479.6328 4886.7 135 3.894 40.0 1 Y.DYLVIGGGSGGVASAR.R * Jamie_Ashy_SPB_28.09922.09922.2 1.5159 0.1794 10.0 1934.4321 1937.126 6960.4 207 3.769 31.2 1 L.SEKSPTRYKIVCAGPNE.K YBL045C 2 2 6.8% 457 50228 7.3 U COR1 SGDID:S000000141, Chr II from 135519-134146, reverse complement, Verified ORF, "Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain" * Jamie_Ashy_SPB_49.17545.17545.3 2.1899 0.0623 52.9 1911.2043 1913.3604 5283.6 425 2.862 28.1 1 K.PVLKKKAAFLGSEVRLR.D * Jamie_Ashy_SPB_9.03266.03266.2 1.3116 0.0031 60.8 1560.0721 1562.7606 5941.9 271 2.847 34.6 1 R.LTISVTDTEVERAK.S YFR031C 3 3 6.7% 1170 133928 7.2 U SMC2 SGDID:S000001927, Chr VI from 220094-216582, reverse complement, Verified ORF, "Component of the condensin complex, essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA, possibly in the cleft formed by the coiled-coil of the folded dimer" * Jamie_Ashy_SPB_36.12854.12854.4 3.096 0.1481 14.1 3176.0166 3181.641 6289.2 1 3.925 24.4 1 F.NAITGLNGSGKSNILDAICFVLGIASMSTVR.A * Jamie_Ashy_SPB_23.08251.08251.2 2.0492 0.0372 14.3 1530.8722 1529.7367 6951.2 8 2.953 54.2 1 P.SSQIIARNEEILR.D * Jamie_Ashy_SPB_60.21290.21290.3 2.9848 0.2843 93.9 3741.5645 3740.4858 8549.6 147 4.622 15.9 1 K.LGNIWKESLIELSGGQRSLIALSLIMALLQFRPA.P Reverse_YDR379W 3 3 6.7% 1009 113291 7.9 U RGA2 SGDID:S000002787, Chr IV from 1230157-1233186, Verified ORF, "GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth" * Jamie_Ashy_SPB_100.35732.35732.4 2.3866 0.061 54.1 4924.7764 4929.507 10911.0 6 3.274 14.8 1 K.KSISGLSSQAKKHNFEPVTFNGHQPLTNYITSDSQGRSVPNKKAK.S * Jamie_Ashy_SPB_10.03331.03331.2 1.6155 0.0499 10.6 915.9922 915.97864 4708.0 3 3.311 68.8 1 R.INTHTSSVG.W * Jamie_Ashy_SPB_8.02808.02808.3 2.3241 0.1264 15.5 1476.7444 1479.587 4440.7 49 4.389 38.5 1 L.NKSIATNNTSVDSK.G Reverse_YAR019C 2 2 6.7% 974 110285 8.0 U CDC15 SGDID:S000000072, Chr I from 175133-172209, reverse complement, Verified ORF, "Protein kinase of the Mitotic Exit Network that is localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p" * Jamie_Ashy_SPB_17.05876.05876.4 2.6557 0.0153 48.5 3491.8965 3491.8591 4406.0 113 2.67 17.8 1 K.QLSGKKSLSRIDECLQCILVSDGGVQNSNRK.P * Jamie_Ashy_SPB_99.35394.35394.4 2.4937 0.1411 15.8 4110.2163 4107.765 6723.0 108 3.7 17.7 1 R.CWFATSTLDYFRYVLELIYKPTKLSAFNPPYMIG.C Reverse_YKR009C 3 3 6.7% 900 98703 9.0 U FOX2 SGDID:S000001717, Chr XI from 456697-453995, reverse complement, Verified ORF, "Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities" * Jamie_Ashy_SPB_61.21985.21985.2 1.6612 0.1224 15.3 2362.3123 2361.662 6112.2 10 3.66 27.5 1 R.VEKEPPVHAGRIFFSGENYAI.L * Jamie_Ashy_SPB_13.04579.04579.3 2.7819 0.0565 74.3 2141.3943 2140.4875 6875.0 264 3.133 26.3 1 K.KTKIDYTEFGGVVLAAKGNK.D * Jamie_Ashy_SPB_7.02404.02404.3 2.3742 0.1445 17.2 2260.1643 2261.4153 4915.9 385 3.487 26.4 1 T.SSPNITNRSFDTIHNWNEK.I YGL073W 2 2 6.7% 833 93282 5.4 U HSF1 SGDID:S000003041, Chr VII from 368756-371257, Verified ORF, "Trimeric heat shock transcription factor, activates multiple genes in response to hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; constitutively DNA-bound; posttranslationally regulated" * Jamie_Ashy_SPB_51.18236.18236.4 2.8756 0.1431 12.7 3239.3364 3234.3823 7765.6 89 3.366 19.4 1 I.PLDNAAGSNNSNNNISSSNSFFNNGHLLQGK.T * Jamie_Ashy_SPB_75.26956.26956.2 1.5639 0.0974 12.0 2930.132 2931.3213 8689.2 256 3.833 18.8 1 E.IPEPAIVEIQDPTEYNDHRLPKRAK.K Reverse_YDL126C 2 2 6.7% 835 91996 4.9 U CDC48 SGDID:S000002284, Chr IV from 238664-236157, reverse complement, Verified ORF, "ATPase in ER, nuclear membrane and cytosol with homology to mammalian p97; in a complex with Npl4p and Ufd1p participates in retrotranslocation of ubiquitinated proteins from the ER into the cytosol for degradation by the proteasome" * Jamie_Ashy_SPB_32.11317.11317.4 2.8953 0.1083 17.4 4768.4565 4772.3867 8208.7 71 3.217 13.6 1 V.TPAAARAKDFIDRINSESEGYWMSLLEPGKVSIFNASVETAVAK.A * Jamie_Ashy_SPB_38.13684.13684.4 3.0552 0.0809 12.8 4511.2964 4515.135 6856.9 265 3.173 13.1 1 R.INSESEGYWMSLLEPGKVSIFNASVETAVAKALLTKGTGPPGY.F YDR258C 2 2 6.7% 811 91336 8.0 U HSP78 SGDID:S000002666, Chr IV from 974237-971802, reverse complement, Verified ORF, "Oligomeric mitochondrial matrix chaperone that cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded matrix proteins; component of the mitochondrial proteolysis system" * Jamie_Ashy_SPB_40.14324.14324.4 3.2324 0.0783 17.4 4350.9365 4355.9907 9259.0 393 3.4 14.9 1 R.PRVANKLLSDIKTNALNEVAISTCALKSSYGLPNFKRTY.V * Jamie_Ashy_SPB_18.06380.06380.2 1.7373 0.1085 15.1 1632.0521 1631.8295 5969.7 16 3.589 42.9 1 V.LSESGGQLTEAVRRK.P Reverse_YCR026C 2 2 6.7% 742 84734 4.6 U NPP1 SGDID:S000000621, Chr III from 166334-164106, reverse complement, Verified ORF, "Nucleotide pyrophosphatase/phosphodiesterase family member; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation" * Jamie_Ashy_SPB_35.12573.12573.4 2.8746 0.0946 11.8 2960.1765 2958.3445 5539.8 4 3.113 22.0 1 H.SGITFVNDENKDKHDGKAVKKLQEKK.M * Jamie_Ashy_SPB_36.12661.12661.4 2.5728 0.0415 54.3 2758.3364 2755.507 5672.0 341 2.507 22.5 1 K.AINIMLILFIAFVLSGFLTRFIIK.F Reverse_YLR069C 2 2 6.7% 761 84574 6.9 U MEF1 SGDID:S000004059, Chr XII from 273916-271631, reverse complement, Verified ORF, "Mitochondrial elongation factor involved in translational elongation" * Jamie_Ashy_SPB_53.19084.19084.3 2.8402 0.1125 16.4 2096.7244 2096.3074 6245.0 11 3.986 32.4 1 T.PDEKQFRNLAKSFNSADK.S * Jamie_Ashy_SPB_83.29605.29605.3 1.6514 0.0086 33.6 3820.6443 3819.4019 8204.0 451 2.668 15.6 1 R.VEHIAKIRKTYYLVRETFTTKGSDIHASIGINR.L YJR005W 2 2 6.7% 700 80453 6.4 U APL1 SGDID:S000003765, Chr X from 445837-447939, Verified ORF, "Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex" * Jamie_Ashy_SPB_94.33691.33691.3 2.4269 0.1407 15.2 2620.5244 2623.0427 4668.3 294 3.578 22.6 1 K.RICHEYIRVIGALKPQQAREAL.P * Jamie_Ashy_SPB_38.13515.13515.3 2.4836 0.1615 17.0 2796.5044 2797.234 7008.4 28 3.512 22.9 1 D.KPAEKINQLKGKRKSSSNNPSKLSR.K Reverse_YOL015W 2 2 6.7% 586 67690 9.7 U YOL015W SGDID:S000005375, Chr XV from 297078-298838, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_19.06792.06792.3 1.8794 0.0066 34.2 1565.6943 1565.7974 5320.3 272 2.886 35.4 1 K.EVCVSSRRMVVSR.V * Jamie_Ashy_SPB_91.32470.32470.2 1.313 0.1099 70.6 3060.612 3062.5828 5521.5 252 3.493 16.0 1 K.NLIKKSIYHLNKTDSDLSLFTKRPTK.E YOL075C 3 3 6.6% 1294 145157 7.1 U YOL075C SGDID:S000005435, Chr XV from 193541-189657, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_90.32139.32139.2 1.9746 0.1726 43.5 2264.0122 2266.6765 9939.7 68 3.941 23.9 1 S.GSVMAVMGGSGSGKTTLLNVLASK.I * Jamie_Ashy_SPB_75.26922.26922.4 2.7288 0.1059 80.4 3250.2166 3246.767 5359.4 7 3.463 21.6 1 R.KFFYQFAVVFLCQLSCSGLSMLSVAVSR.D * Jamie_Ashy_SPB_53.18818.18818.4 3.1951 0.1576 19.4 3677.6965 3682.2317 8711.3 47 3.47 18.8 1 R.SELFKRFGNVLLLAKSGRTAFNGSPDEMIAYFT.E YHR027C 2 2 6.6% 993 109492 4.6 U RPN1 SGDID:S000001069, Chr VIII from 164704-161723, reverse complement, Verified ORF, "Non-ATPase base subunit of the 19S regulatory particle of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein?protein interactions" * Jamie_Ashy_SPB_63.22438.22438.3 2.3195 0.0456 47.0 2948.0645 2952.215 5564.0 102 2.884 21.7 1 K.HDSLRYRLLSDVSDFEGWGHEYIR.H * Jamie_Ashy_SPB_72.25687.25687.4 2.4353 0.2152 10.3 4737.5366 4733.322 5828.0 39 3.866 15.9 1 E.EDMIRSVFDATSDPVMHKQLAYILAAQKTSFEYEGVQDIIGN.G Reverse_YMR080C 3 3 6.6% 971 109430 6.5 U NAM7 SGDID:S000004685, Chr XIII from 429626-426711, reverse complement, Verified ORF, "ATP-dependent RNA helicase of the SFI superfamily, required for nonsense mediated mRNA decay and for efficient translation termination at nonsense codons" * Jamie_Ashy_SPB_111.39873.39873.2 1.6765 0.1194 14.8 1967.4122 1970.0973 7328.7 417 3.632 20.6 1 P.PIAEQEEPADIDNITADK.N * Jamie_Ashy_SPB_43.15249.15249.3 2.6207 0.1091 11.4 2528.8743 2531.6636 8947.6 17 3.671 26.2 1 R.DEILPQWQDTDWNANKTQACP.I * Jamie_Ashy_SPB_118.42186.42186.3 2.5755 0.1253 13.5 2821.2844 2816.9026 8479.5 201 3.455 21.9 1 R.FGTETVQAEEFLQNDVDDDEVLTSN.L YDR395W 3 3 6.6% 944 108405 4.7 U SXM1 SGDID:S000002803, Chr IV from 1263314-1266148, Verified ORF, "Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1" * Jamie_Ashy_SPB_56.20176.20176.2 1.5362 0.0838 74.8 1777.1122 1778.9164 6965.2 16 2.987 39.3 1 R.SWDTKREDGIKADEK.L * Jamie_Ashy_SPB_96.34257.34257.4 3.0369 0.096 17.3 3757.1365 3761.188 6009.3 8 3.695 20.0 1 R.EFFKDIEAWGNNSLWLSDSSLYFLISFLEKC.V * Jamie_Ashy_SPB_13.04372.04372.2 2.4581 0.1261 48.0 1737.9321 1739.9664 5747.3 212 3.742 36.7 1 P.EVPNSVSPLLNNLTNK.L YCR030C 2 2 6.6% 870 96137 8.6 U SYP1 SGDID:S000000626, Chr III from 176433-173821, reverse complement, Verified ORF, "Protein with a potential role in actin cytoskeletal organization; overexpression suppresses a pfy1 (profilin) null mutation" * Jamie_Ashy_SPB_44.15561.15561.4 3.22 0.0438 75.6 4075.3364 4078.5027 6675.0 46 3.111 18.7 1 R.FQTSFSDYLLNTTKECETVMTKFLAFEPQSEIDR.F * Jamie_Ashy_SPB_55.19503.19503.2 1.4265 0.1412 12.5 2645.4722 2644.0427 7764.8 50 3.706 22.7 1 P.EEFKVNPSFIDSRTLGAIKYSIK.E Reverse_YER162C 2 2 6.6% 754 87204 7.8 U RAD4 SGDID:S000000964, Chr V from 502889-500625, reverse complement, Verified ORF, "Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); homolog of human XPC protein" * Jamie_Ashy_SPB_70.24978.24978.4 2.9869 0.1805 25.5 3606.5764 3610.1538 5714.0 101 3.626 19.5 1 K.SRIRLKLLLTNWSELAGLLHEKRNDDEQIF.E * Jamie_Ashy_SPB_48.17037.17037.4 2.2675 0.0064 39.0 2294.1365 2299.5894 4966.3 24 2.913 28.1 1 R.EDEEEAEGKPRGVTRKIVKK.C YGL114W 2 2 6.6% 725 80009 6.7 U YGL114W SGDID:S000003082, Chr VII from 293463-295640, Uncharacterized ORF, "Putative member of the oligopeptide transporter (OPT) family of membrane transporters" * Jamie_Ashy_SPB_28.09844.09844.4 3.1228 0.1489 10.6 3712.9365 3717.071 5997.9 264 3.472 17.2 1 S.KSELLEMRQRRLNECPEVLQPNRDPEEADY.L * Jamie_Ashy_SPB_54.19213.19213.2 2.5837 0.2082 23.1 1958.9922 1960.4081 9006.9 9 4.513 41.2 1 I.VVTVKSIVKFILIDDKAA.L Reverse_YNL293W 2 2 6.6% 633 72999 7.6 U MSB3 SGDID:S000005237, Chr XIV from 80640-82541, Verified ORF, "GTPase-activating protein for Sec4p and several other Rab GTPases, regulates exocytosis via its action on Sec4p, also required for proper actin organization; similar to Msb4p; both Msb3p and Msb4p localize to sites of polarized growth" * Jamie_Ashy_SPB_23.08178.08178.2 2.4489 0.035 16.0 1776.3322 1775.0364 7006.6 5 2.936 40.0 1 K.VCLMLVGQDINVGELN.I * Jamie_Ashy_SPB_44.15768.15768.3 2.1822 0.011 36.6 3045.7144 3047.403 8305.9 321 2.36 21.0 1 K.IIPPGEKNHLSSQFHINDPFTRNLDR.E YOR324C 2 2 6.6% 602 67293 9.4 U FRT1 SGDID:S000005851, Chr XV from 925035-923227, reverse complement, Verified ORF, "Tail-anchored endoplasmic reticulum membrane protein that is a substrate of the phosphatase calcineurin, interacts with homolog Frt2p, promotes cell growth in conditions of high Na+, alkaline pH, and cell wall stress" * Jamie_Ashy_SPB_25.08740.08740.2 1.6009 0.1555 16.6 1683.3322 1685.7875 6145.3 84 3.361 36.7 1 R.SYSGASSLDDPFRVSP.K * Jamie_Ashy_SPB_40.14094.14094.4 3.3495 0.0717 19.1 2804.1765 2804.278 8117.3 2 2.94 26.1 1 L.NKQKSSLREMDSLIELKNVLNKSK.N YMR093W 2 2 6.6% 513 57686 9.3 U UTP15 SGDID:S000004699, Chr XIII from 454014-455555, Verified ORF, "Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA" * Jamie_Ashy_SPB_17.06019.06019.2 2.21 0.0852 19.8 1513.8922 1514.852 4910.2 268 3.674 42.9 1 L.VAGLSSGLLAIRTKK.K * Jamie_Ashy_SPB_4.01467.01467.3 2.8211 0.049 73.8 2125.4043 2122.3022 4623.2 174 3.84 26.4 1 K.EKRSSDKENAPASFNKNAK.S Reverse_YOL042W 2 2 6.6% 363 42398 8.2 U NGL1 SGDID:S000005402, Chr XV from 249823-250914, Verified ORF, "Putative endonuclease, has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p" * Jamie_Ashy_SPB_19.06867.06867.2 1.6188 0.0962 84.6 1225.5122 1224.5297 5830.6 77 3.345 55.0 1 K.VDGPLLQKIIK.S * Jamie_Ashy_SPB_47.16758.16758.2 1.9719 0.1523 27.8 1750.7322 1752.15 9697.2 355 4.264 34.6 1 I.KSLERMIIMCQTLK.V Reverse_YKL129C 4 4 6.5% 1271 142507 9.4 U MYO3 SGDID:S000001612, Chr XI from 200163-196348, reverse complement, Verified ORF, "One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization" * Jamie_Ashy_SPB_81.29023.29023.4 3.3104 0.1102 13.6 4024.6965 4019.0393 6674.9 7 3.866 17.6 1 -.WDDDDDGDDNDDDDASEARMKNARAALASALGDSFTGV.P * Jamie_Ashy_SPB_81.29029.29029.4 2.8009 0.105 14.2 3313.2966 3318.8533 5653.4 23 3.313 19.4 1 K.SKIPKGSLGGPNKPKKNSKSNAPHGRRVYIT.S * Jamie_Ashy_SPB_14.05047.05047.2 1.4715 0.0436 66.1 1196.7122 1196.3114 5480.4 348 3.128 55.0 1 K.KNSKSNAPHGR.R * Jamie_Ashy_SPB_16.05588.05588.3 2.4339 0.1017 12.9 1573.3744 1576.8856 4469.9 19 3.198 39.6 1 D.RLKVYKNGGKKER.I Reverse_YGR218W 3 3 6.5% 1084 124104 5.5 U CRM1 SGDID:S000003450, Chr VII from 932544-935798, Verified ORF, "Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus" * Jamie_Ashy_SPB_10.03513.03513.2 1.4247 0.1236 11.5 1877.7922 1878.3673 7394.1 93 3.596 34.4 1 V.LSILKMLLLAQKSFGSK.H * Jamie_Ashy_SPB_108.38672.38672.4 2.4355 0.1395 13.6 4581.0967 4584.467 8279.7 215 3.725 14.5 1 L.FRPYQGVVYMIDSAVVAKNDKGRKKVTLDLLDKIVTVVFR.K * Jamie_Ashy_SPB_84.30042.30042.2 1.7726 0.1301 93.1 1479.1921 1481.7502 6510.2 353 4.103 37.5 1 K.LTIARTDPSTMFK.T YNL127W 2 2 6.5% 953 109793 5.6 U FAR11 SGDID:S000005071, Chr XIV from 383989-386850, Verified ORF, "Protein involved in G1 cell cycle arrest in response to pheromone, in a pathway different from the Far1p-dependent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p" * Jamie_Ashy_SPB_4.01191.01191.4 2.7013 0.1437 16.9 3085.0964 3089.641 4889.4 241 3.728 20.8 1 K.NPLNMALIVPEKHIATPAPSPPSSPQLMH.L * Jamie_Ashy_SPB_108.38878.38878.3 1.6814 0.0627 35.3 3774.6243 3770.272 8630.8 61 3.165 14.8 1 R.IDLNIERKPDNDIVIPFSTEEAARILSESLEIK.L Reverse_YLR357W 2 2 6.5% 889 102300 8.9 U RSC2 SGDID:S000004349, Chr XII from 841330-843999, Verified ORF, "One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook" * Jamie_Ashy_SPB_59.21164.21164.2 1.7241 0.0776 13.1 1966.0521 1964.1857 5964.9 6 2.949 40.6 1 S.TLDDIITPINYRSAQSR.K * Jamie_Ashy_SPB_50.17806.17806.3 2.0198 0.1008 52.7 4280.9043 4279.615 8002.6 2 4.17 15.6 1 R.LSSLKSSSYGTKPLANATPTNTTITGTEIDVQGEEPSDNPR.I Reverse_contaminant_GR78_KLULA 2 2 6.5% 679 74448 4.9 U owl|P22010| 78 KD GLUCOSE REGULATED PROTEIN HOMOLOG PRECURSOR (GRP 78) (IMMUNOGLOBULIN... Jamie_Ashy_SPB_2.00611.00611.2 1.997 0.1196 97.0 900.5722 901.0537 5479.4 1 3.682 85.7 1 K.KTPIATNR.K * Jamie_Ashy_SPB_50.17943.17943.4 3.1742 0.0782 15.3 4145.177 4141.584 7078.5 331 3.003 15.7 1 L.EEFKARTLTESFDIGNFFSDIEIRTSTQSSLSRKAK.E Reverse_YPR161C 2 2 6.5% 657 74239 9.5 U SGV1 SGDID:S000006365, Chr XVI from 866418-864445, reverse complement, Verified ORF, "Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p, which is the largest subunit of RNA polymerase II; regulated by Cak1p" * Jamie_Ashy_SPB_23.08260.08260.4 2.9044 0.0131 55.8 2603.5366 2607.8743 5057.8 3 2.784 25.8 1 R.KIDAEHSEETPLTIKESPLPDEK.F * Jamie_Ashy_SPB_7.02352.02352.4 2.8085 0.1139 87.3 2096.0166 2099.3196 5291.4 47 3.076 30.7 1 K.AGSGAGGPYKLNPPCGYYLR.A Reverse_YGR194C 2 2 6.5% 600 68321 6.8 U XKS1 SGDID:S000003426, Chr VII from 887880-886078, reverse complement, Verified ORF, "Xylulokinase, converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains" * Jamie_Ashy_SPB_109.39019.39019.3 2.7645 0.1272 18.0 2583.3245 2580.9128 5576.5 458 4.075 22.8 1 G.GLACSNPTELRFNGKTAGIVQAFK.K * Jamie_Ashy_SPB_22.07803.07803.2 1.6118 0.034 67.2 1604.9521 1603.7294 5445.9 20 2.747 42.9 1 R.QQSSANSDSLLPSIR.V contaminant_SPA1_STAAU 3 4 6.5% 524 57320 5.7 U owl|P02976| IMMUNOGLOBULIN G BINDING PROTEIN A PRECURSOR (PROTEIN A). - STAPHYLOCOCCUS... contaminant_SPA2_STAAU 3 4 6.7% 508 55439 5.7 U owl|P38507| IMMUNOGLOBULIN G BINDING PROTEIN A PRECURSOR (PROTEIN A). - STAPHYLOCOCCUS... Jamie_Ashy_SPB_45.16080.16080.2 3.6623 0.4311 100.0 2485.652 2486.7031 6530.9 1 6.307 47.4 2 K.EQQNAFYEILHLPNLNEEQR.N Jamie_Ashy_SPB_45.16104.16104.3 4.0164 0.254 100.0 2489.5444 2486.7031 7867.4 1 5.391 31.6 1 K.EQQNAFYEILHLPNLNEEQR.N Jamie_Ashy_SPB_20.06986.06986.1 2.8303 0.2018 100.0 1459.68 1459.5529 3722.7 1 4.767 61.5 1 K.DDPSQSANLLAEAK.K Reverse_YOR123C 2 2 6.5% 464 53920 4.4 U LEO1 SGDID:S000005649, Chr XV from 554570-553176, reverse complement, Verified ORF, "Component of the Paf1 complex, which associates with RNA polymerase II and is involved in histone methylation" * Jamie_Ashy_SPB_3.01106.01106.4 2.3652 0.1121 14.5 1602.3365 1597.9076 4230.3 4 3.769 34.7 1 S.QRQNRRIVAKSLK.Q * Jamie_Ashy_SPB_104.37272.37272.2 1.6965 0.1027 17.7 2006.4922 2006.0991 6538.9 344 3.481 28.1 1 K.IEGGEYCQILEQQDHSV.T Reverse_YER043C 2 2 6.5% 449 49126 6.2 U SAH1 SGDID:S000000845, Chr V from 237118-235769, reverse complement, Verified ORF, "S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor" * Jamie_Ashy_SPB_32.11449.11449.2 1.7269 0.1305 16.0 1483.2522 1483.8002 5567.7 184 3.176 45.8 1 L.KTLRVGLNGLHFK.A * Jamie_Ashy_SPB_19.06862.06862.2 1.9812 0.0633 85.9 1713.9521 1714.0153 5501.2 392 3.075 36.7 1 K.SKTVSDNVNIAPVKLK.G YOR326W 3 3 6.4% 1574 180679 6.3 U MYO2 SGDID:S000005853, Chr XV from 925716-930440, Verified ORF, "One of two type V myosins, involved in polarized distribution of mitochondria; required for mitochondrion and vacuole inheritance and nuclear spindle orientation; moves multiple cargo" * Jamie_Ashy_SPB_42.15153.15153.4 3.1117 0.1418 10.2 2544.6165 2541.006 4872.1 32 3.699 25.8 1 I.TVDALTLVGITKETQHQIFKILA.A * Jamie_Ashy_SPB_65.23331.23331.3 1.8497 0.029 33.5 4311.7144 4308.8877 10063.8 335 2.883 14.9 1 R.NDASLSADEPNLKLACELLGIDAYNFAKWVTKKQIITR.S * Jamie_Ashy_SPB_52.18750.18750.4 3.8565 0.0212 11.9 4292.8164 4293.9053 7422.7 8 3.654 15.8 1 N.RDTVSDGHLEVLKASTNETLINILEGLEKAAKKLEEAKK.L YKL010C 3 3 6.4% 1483 167842 4.9 U UFD4 SGDID:S000001493, Chr XI from 425518-421067, reverse complement, Verified ORF, "Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins" * Jamie_Ashy_SPB_21.07346.07346.2 1.5253 0.1104 12.0 2545.892 2542.8977 5866.3 3 3.49 28.6 1 K.FDSLVQCLIPILVEIYTNAADF.D * Jamie_Ashy_SPB_89.31869.31869.3 2.5239 0.1441 18.1 2405.7544 2404.6482 7292.2 4 3.714 28.4 1 E.NFSIVDCGLHDGGGVSSLAKEIK.I * Jamie_Ashy_SPB_89.32073.32073.4 2.2517 0.176 18.3 5760.7764 5761.4136 9738.6 1 3.901 14.3 1 D.ELVDIFGRVEEDWSMATLYTNLNAEHGYTMDSSIIHDFISIISAFGKHER.R YDL102W 2 2 6.4% 1097 124591 8.0 U CDC2 SGDID:S000002260, Chr IV from 276872-280165, Verified ORF, "Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)" * Jamie_Ashy_SPB_64.22976.22976.4 2.3221 0.1983 13.7 5439.8965 5441.092 8781.9 3 4.001 13.2 1 K.KIKRQSIDHGVGSEPVSTIEIIPSDSFRKYNSQGFKAKDTDLMGTQLES.T * Jamie_Ashy_SPB_116.41636.41636.2 1.0942 0.0578 60.9 2299.1921 2297.6843 6137.3 12 3.314 22.5 1 K.INTGSQKGGLMSFIKKVEACK.S Reverse_YML075C 2 2 6.4% 1054 115625 8.0 U HMG1 SGDID:S000004540, Chr XIII from 118898-115734, reverse complement, Verified ORF, "One of two isozymes of HMG-CoA reductase that catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; localizes to the nuclear envelope; overproduction induces the formation of karmellae" * Jamie_Ashy_SPB_59.21181.21181.4 3.4608 0.0477 12.1 4209.0166 4204.86 6400.5 367 3.333 15.0 1 W.IKCAGSRKLTPFRVVPGRTMGDKTLVTTAGGGANIAKCGR.M * Jamie_Ashy_SPB_38.13562.13562.3 2.9491 0.1006 17.1 2977.8542 2980.3916 7251.9 42 3.794 20.2 1 K.EAKSIIRATSPVVGDEELTQKIITSRH.I YMR273C 2 2 6.4% 915 103358 6.4 U ZDS1 SGDID:S000004886, Chr XIII from 813979-811232, reverse complement, Verified ORF, "Protein that interacts with silencing proteins at the telomere, involved in transcriptional silencing; has a role in localization of Bcy1p, a regulatory subunit of protein kinase A; implicated in mRNA nuclear export" * Jamie_Ashy_SPB_17.06108.06108.3 2.8077 0.2112 30.3 2737.3743 2739.0142 4803.1 190 3.976 21.7 1 M.ELDNSKKHYISLFNGGEKTEVSNK.E * Jamie_Ashy_SPB_58.20898.20898.4 3.3219 0.0652 15.7 4089.4565 4086.6238 7692.5 456 3.395 16.7 1 K.SKKLGNKSGREPVEPIVLRNRPRPHRHHHSRHGSQ.K YHR182W 2 2 6.4% 785 90109 6.4 U YHR182W SGDID:S000001225, Chr VIII from 468219-470576, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm" * Jamie_Ashy_SPB_38.13464.13464.3 2.6376 0.1209 15.2 3170.1543 3170.3826 5376.9 86 3.585 19.6 1 K.ANTPVENSSSVFDNGKDSAPLTRLNFVCE.F * Jamie_Ashy_SPB_63.22650.22650.2 1.4393 0.1545 15.0 2483.9321 2481.863 9545.4 308 4.002 27.5 1 G.HFIPRPFKTSSTPTTPERPKR.K Reverse_YLR054C 3 3 6.4% 724 82328 6.7 U OSW2 SGDID:S000004044, Chr XII from 250979-250949,250861-248718, reverse complement, Uncharacterized ORF, "Protein of unknown function proposed to be involved in the assembly of the spore wall" * Jamie_Ashy_SPB_53.19084.19084.2 1.8865 0.1292 23.4 1398.1522 1400.5748 6245.0 13 3.645 54.2 1 R.LIDGTNGRNLVDI.H * Jamie_Ashy_SPB_104.37172.37172.2 1.7572 0.059 12.8 1771.5521 1773.0427 6206.0 172 3.091 34.4 1 R.RVNSGKIGQLELSAAKT.N * Jamie_Ashy_SPB_39.14025.14025.2 2.8089 0.0511 96.8 1808.6122 1809.1155 7434.9 19 3.179 43.3 1 K.IDKTLINPTYFNAGLK.T Reverse_YMR232W 2 2 6.4% 677 79037 8.9 U FUS2 SGDID:S000004845, Chr XIII from 736925-738958, Verified ORF, "Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating" * Jamie_Ashy_SPB_10.03614.03614.4 2.1729 0.0358 40.8 3495.5764 3494.22 6278.0 263 3.059 18.5 1 R.KIDRVNAKKEQLFLMYDKKLAQLDKLKSK.V * Jamie_Ashy_SPB_40.14136.14136.2 1.885 0.0792 15.8 1738.7522 1736.0737 6555.3 390 3.54 42.3 1 N.LKNEHKSLKRTPKR.V Reverse_YLR427W 2 2 6.4% 670 76317 8.5 U MAG2 SGDID:S000004419, Chr XII from 988424-990436, Uncharacterized ORF, "DNA-3-methyladenine glycosidase II that catalyzes of the hydrolysis of alkylated DNA" * Jamie_Ashy_SPB_91.32797.32797.4 2.5803 0.1217 11.1 4497.5767 4495.8823 6705.8 361 3.318 16.2 1 K.KKRGRKGNSTPPSDTSDTVDSSDEQRIRQLLMDQFEKNE.K * Jamie_Ashy_SPB_25.08936.08936.4 2.1879 0.0262 39.2 4501.6167 4497.8516 6976.6 14 3.123 15.8 1 R.GRKGNSTPPSDTSDTVDSSDEQRIRQLLMDQFEKNEKSAK.E Reverse_YAR018C 2 2 6.4% 435 51203 8.4 U KIN3 SGDID:S000000071, Chr I from 171698-170391, reverse complement, Verified ORF, "Nonessential protein kinase with unknown cellular role" * Jamie_Ashy_SPB_1.00213.00213.2 1.3932 0.0148 62.3 1260.8722 1262.3246 1786.1 261 3.044 33.3 1 R.SYYEPVTDCK.G * Jamie_Ashy_SPB_56.20052.20052.2 1.808 0.0935 15.6 2091.4321 2091.2688 6677.5 3 3.237 35.3 1 V.QSYDMPSGRKGSNVRHDR.Y YMR083W 2 2 6.4% 375 40370 8.4 U ADH3 SGDID:S000004688, Chr XIII from 434787-435914, Verified ORF, "Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production" * Jamie_Ashy_SPB_43.15520.15520.3 2.5806 0.135 16.6 1865.2743 1867.1272 4523.0 1 3.481 37.5 1 T.AMGYRVLGIDAGEEKEK.L Jamie_Ashy_SPB_7.02442.02442.1 1.9183 0.0696 96.7 812.35 811.9103 5388.2 19 3.092 75.0 1 R.YVVDTSK.- YHL023C 2 2 6.3% 1146 129973 6.4 U RMD11 SGDID:S000001015, Chr VIII from 62561-59121, reverse complement, Verified ORF, "Protein required for sporulation" * Jamie_Ashy_SPB_17.06037.06037.4 2.8287 0.1048 15.6 4513.4165 4517.8228 8520.7 15 3.361 14.2 1 R.TNSSFQSTDSMSPTSPQMIMENDSISVAESYLDSGTNNKSRA.A * Jamie_Ashy_SPB_7.02434.02434.4 2.0998 0.0125 35.7 3794.1765 3788.351 4307.7 172 3.316 17.7 1 K.SRAASKRSQNFFHKLSTKKSTDSKTHSPVRKLK.S Reverse_YBL037W 2 2 6.3% 1025 115011 8.6 U APL3 SGDID:S000000133, Chr II from 147212-150289, Verified ORF, "Alpha-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport" * Jamie_Ashy_SPB_7.02467.02467.4 2.7945 0.1105 15.3 2408.7366 2405.575 4486.3 30 3.836 27.1 1 K.SLLDPTSSSAESPTNILTTSGASAP.L * Jamie_Ashy_SPB_71.25279.25279.4 3.5961 0.1406 22.5 4438.2964 4441.092 8893.6 1 3.392 18.4 1 V.SVCKRLKNLLENDINNTQLLSGSDRETPSAILISLLSVVK.T Reverse_YPL183C 3 3 6.3% 1013 114461 6.2 U YPL183C SGDID:S000006104, Chr XVI from 202535-199494, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_69.24814.24814.2 1.7833 0.0827 13.4 2504.0122 2505.0208 8377.9 4 3.453 31.0 1 S.YKLIKGLSTIAIVGFSFWQFGK.I * Jamie_Ashy_SPB_20.07150.07150.3 2.3176 0.1683 18.1 3590.1243 3590.0425 5262.3 475 4.158 16.7 1 I.MEDDKVDVGWISKILHVEYVNSISLEAVNER.S * Jamie_Ashy_SPB_36.12816.12816.4 3.2138 0.0729 15.6 3500.8164 3505.8848 9298.2 19 3.893 21.4 1 K.ILHVEYVNSISLEAVNERSEIINWVRCTC.D Reverse_YMR266W 2 2 6.3% 953 107672 8.6 U YMR266W SGDID:S000004879, Chr XIII from 798517-801378, Uncharacterized ORF, "Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant" * Jamie_Ashy_SPB_69.24798.24798.4 3.4549 0.1548 29.1 4569.3364 4564.7466 8585.7 67 3.83 15.4 1 -.VASRSEEEKYKPDAFPNNEKSATDFEGNAEGNAEDKVDNYP.P * Jamie_Ashy_SPB_119.42676.42676.2 1.0723 0.0352 59.8 2121.9722 2119.3794 10179.4 220 3.093 27.8 1 R.KKDPVYEAINDSVSLQPSK.K YGL008C 2 2 6.3% 918 99619 5.1 U PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases" YPL036W 2 2 6.1% 947 102172 5.0 U PMA2 SGDID:S000005957, Chr XVI from 482841-485684, Verified ORF, "Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential" Jamie_Ashy_SPB_24.08427.08427.2 3.2792 0.4753 100.0 3021.7322 3022.2114 6509.5 1 8.026 34.0 1 K.TVEEDHPIPEDVHENYENKVAELASR.G Jamie_Ashy_SPB_59.21151.21151.3 3.3721 0.1193 91.2 3263.4844 3263.5623 7593.4 12 3.836 21.0 1 R.LGLGGGGDMPGSELADFVENADGFAEVFPQHK.Y Reverse_YLR103C 2 2 6.3% 650 74249 4.8 U CDC45 SGDID:S000004093, Chr XII from 345942-343990, reverse complement, Verified ORF, "DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes" * Jamie_Ashy_SPB_8.02923.02923.4 2.4773 0.0124 42.6 3055.9766 3058.372 5837.3 205 2.756 17.9 1 K.DTSNGVELLATLAEVFESASISGRYGLTR.V * Jamie_Ashy_SPB_7.02408.02408.2 2.0193 0.0962 94.0 1434.4321 1433.6476 5487.2 3 3.439 59.1 1 R.DLNKDFIIGLER.K YMR278W 2 2 6.3% 622 71069 6.3 U YMR278W SGDID:S000004891, Chr XIII from 822762-824630, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_97.34746.34746.2 1.7771 0.0557 13.2 2190.2122 2190.5498 9086.9 30 3.692 33.3 1 N.PDHEFVHTPLVPFAVDKLK.A * Jamie_Ashy_SPB_118.42364.42364.3 2.3547 0.1654 16.5 2258.3342 2260.462 6207.7 69 3.595 25.0 1 T.PEGASYPSSIGEEIEVLYYR.D Reverse_YDR523C 2 2 6.3% 490 55704 7.7 U SPS1 SGDID:S000002931, Chr IV from 1487028-1485556, reverse complement, Verified ORF, "Putative protein serine/threonine kinase expressed at the end of meiosis and localized to the prospore membrane, required for correct localization of enzymes involved in spore wall synthesis" * Jamie_Ashy_SPB_65.23233.23233.3 2.25 0.0241 40.0 3594.1743 3597.1924 10064.7 168 3.148 17.5 1 K.KQKILDVDSKLNTITINKVFEFSLLNYASPR.D * Jamie_Ashy_SPB_99.35368.35368.4 3.1102 0.0925 18.8 2806.4165 2801.2524 4136.0 198 3.625 23.2 1 K.ILDVDSKLNTITINKVFEFSLLNY.A YDR283C 4 4 6.2% 1659 190193 6.4 U GCN2 SGDID:S000002691, Chr IV from 1030043-1025064, reverse complement, Verified ORF, "Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex" * Jamie_Ashy_SPB_92.32896.32896.2 1.4033 0.0306 63.6 2229.652 2229.4944 6896.8 4 2.988 27.5 1 R.YASDFEEIAVLGQGAFGQVVK.A * Jamie_Ashy_SPB_114.40872.40872.3 1.3866 0.0458 33.7 3410.9944 3410.0154 6761.2 165 3.473 10.7 1 K.KIRHTEEKLSTILSEVMLLASLNHQYVVR.Y * Jamie_Ashy_SPB_102.36456.36456.3 2.1971 0.0068 39.3 1964.6044 1961.3091 4250.5 248 2.658 29.7 1 K.KIEDSLSHISKVLSYLK.P * Jamie_Ashy_SPB_112.40114.40114.4 1.9621 0.0196 33.9 4118.3765 4112.6562 5105.6 1 2.79 18.6 1 K.ANKREKWVYEDAARNSSNMILHNLSNAPIITVDALR.D YNR067C 2 2 6.2% 1117 121064 4.5 U DSE4 SGDID:S000005350, Chr XIV from 759099-755746, reverse complement, Verified ORF, "Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother" * Jamie_Ashy_SPB_62.22110.22110.4 3.5014 0.1191 10.1 4362.8164 4360.9053 9674.0 455 3.165 14.9 1 L.TLLFLLSFVECSYISFISNNADEILETDLIETLSYATL.T * Jamie_Ashy_SPB_67.24012.24012.4 2.9013 0.1126 18.9 3678.2566 3673.1345 7076.0 394 3.366 18.9 1 T.EYIHGIHMLPITPVSSNIRSETFVEEEWQTK.I Reverse_YOR127W 2 2 6.2% 1007 112831 6.4 U RGA1 SGDID:S000005653, Chr XV from 561170-564193, Verified ORF, "GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth" * Jamie_Ashy_SPB_14.04810.04810.3 1.8935 0.0982 46.7 3684.2944 3687.0122 5155.9 159 3.251 16.7 1 R.GSNSGSSPASLLVPASKVISNTNNTSVGQISQETQPK.A * Jamie_Ashy_SPB_33.11866.11866.2 1.3649 0.1213 75.8 2808.3523 2809.1575 8089.1 13 3.579 22.9 1 K.ALYQGSSNRLPTKNLLNRSHSPPEK.C Reverse_YOR206W 2 2 6.2% 710 81601 8.5 U NOC2 SGDID:S000005732, Chr XV from 727512-729644, Verified ORF, "Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors" * Jamie_Ashy_SPB_72.25864.25864.2 1.7 0.144 19.8 1548.1721 1545.8436 5414.9 80 3.706 45.5 1 E.RMLRAKEEKVER.Q * Jamie_Ashy_SPB_83.29920.29920.4 3.7109 0.2074 73.8 3377.8564 3382.008 8172.4 59 4.03 20.4 1 K.VISSVRSVNGGNPLTRAGKVIKYPAMKQIAQP.V YML017W 2 2 6.2% 593 65584 8.7 U PSP2 SGDID:S000004479, Chr XIII from 236588-236591,236954-238731, Verified ORF, "Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing" * Jamie_Ashy_SPB_119.42537.42537.2 1.2821 0.1762 16.0 960.8122 963.0617 6232.3 238 3.691 50.0 1 P.HGGHGPMNR.S * Jamie_Ashy_SPB_60.21398.21398.2 1.342 0.0276 61.4 2997.5923 2997.1414 9199.2 1 3.138 24.1 1 R.GSNRYRGGPNGSSYKGGHNNRGNRGGYR.G Reverse_YLR419W 3 3 6.1% 1435 163044 9.1 U YLR419W SGDID:S000004411, Chr XII from 958424-962731, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_29.10356.10356.3 2.2896 0.1297 15.4 1625.8444 1627.9213 4227.9 43 4.033 34.6 1 R.LYLKNTVQSTTFLV.F * Jamie_Ashy_SPB_90.32211.32211.4 2.9928 0.1834 20.0 4694.3765 4688.541 7903.0 7 4.062 15.8 1 M.AKVSLYLSELPIRKIEPIPMSIMDNEYLNKSFLKYSLGER.V * Jamie_Ashy_SPB_97.34867.34867.4 2.6595 0.0432 57.1 3791.6565 3797.2651 8292.5 442 3.123 17.7 1 R.SSEDLDVFISNEWVKKPFRVLSPNHSNKHSAVK.R Reverse_YDR216W 3 3 6.1% 1323 150940 6.7 U ADR1 SGDID:S000002624, Chr IV from 895029-899000, Verified ORF, "Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization" * Jamie_Ashy_SPB_31.10954.10954.4 3.8144 0.2626 36.4 3653.7366 3657.21 7385.7 37 4.325 19.3 1 F.RMSMLNNKLIITDDILIAINKNTAAMNDNGYSS.T * Jamie_Ashy_SPB_43.15454.15454.4 2.7994 0.0015 47.7 3991.7366 3996.475 7877.1 12 2.738 17.6 1 R.DKPHQELSSQDPLAYSSASQASFSARRTLKKLYKR.K * Jamie_Ashy_SPB_18.06469.06469.2 1.9341 0.0798 15.6 1685.2722 1685.8423 9053.4 84 2.924 41.7 1 C.PYPKENTHSRYHR.K Reverse_YMR076C 3 3 6.1% 1277 147041 6.3 U PDS5 SGDID:S000004681, Chr XIII from 420028-416195, reverse complement, Verified ORF, "Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes in an interdependent manner, may function as a protein-protein interaction scaffold" * Jamie_Ashy_SPB_66.23529.23529.3 1.4252 0.1121 35.6 5184.5645 5188.3916 4565.3 102 3.514 8.3 1 K.MVINEHGKKTENKNSPSYSDDDSDSERDGDGEEDDAQSRAHHVKKK.K * Jamie_Ashy_SPB_39.14022.14022.2 2.0152 0.0823 18.2 1384.1522 1385.7025 9178.4 313 3.544 45.5 1 L.KLVQIGAVCRLK.T * Jamie_Ashy_SPB_66.23745.23745.2 1.9344 0.1514 33.1 2288.7522 2287.6182 8492.5 414 3.961 23.7 1 E.VWDIEKKSDGVVQNSLLVEK.I YOR188W 3 3 6.1% 1137 129971 8.9 U MSB1 SGDID:S000005714, Chr XV from 685767-689180, Verified ORF, "Protein involved in positive regulation of both 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway, potential Cdc28p substrate; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4" * Jamie_Ashy_SPB_96.34400.34400.2 1.7863 0.0673 12.4 1524.8322 1524.7816 4893.8 228 3.035 37.5 1 R.KSFLEIMPQCLAS.P * Jamie_Ashy_SPB_47.16857.16857.2 2.337 0.0822 25.6 2117.7522 2120.3208 6494.3 7 3.513 36.1 1 K.KKTSAQSPTTEKELPPDDF.E * Jamie_Ashy_SPB_39.14029.14029.4 2.6292 0.1353 19.3 4416.0967 4411.0747 6024.5 68 3.671 16.7 1 G.IYHTVISKNALTKNKHNVNLHSFEHKISKWNPLNNLR.K Reverse_YJL020C 3 3 6.1% 1157 128296 5.3 U BBC1 SGDID:S000003557, Chr X from 402326-398853, reverse complement, Verified ORF, "Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches" * Jamie_Ashy_SPB_18.06217.06217.4 2.0211 0.0077 35.5 2074.2566 2078.4395 5393.1 335 3.174 26.5 1 K.RFFPNSSPDVFPMVPIAR.P * Jamie_Ashy_SPB_67.24020.24020.2 1.6685 0.1281 13.8 1768.1522 1771.0702 6694.6 450 3.495 34.6 1 E.QELKAKKRLLEEER.A * Jamie_Ashy_SPB_53.18938.18938.4 3.742 0.2616 16.7 4563.0166 4560.792 6823.4 199 4.251 16.7 1 P.FIGEIVDGNSDQYEGFYWEADEVSTVIIEQDKEFNLDDE.Y YDR166C 2 2 6.1% 971 112121 5.8 U SEC5 SGDID:S000002573, Chr IV from 789216-786301, reverse complement, Verified ORF, "Essential 107kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis" * Jamie_Ashy_SPB_27.09726.09726.4 3.4632 0.1771 45.7 4667.9365 4673.06 12353.4 109 3.741 14.6 1 R.FYQLKTINPTHSWAQDSSKLNNEEATSNELGVETSFDILKD.F * Jamie_Ashy_SPB_15.05103.05103.4 2.225 0.0653 54.8 2106.0566 2111.4587 5473.7 118 2.987 27.5 1 R.LQKCINEINPIVSANLKR.T YNL236W 2 2 6.1% 974 111297 8.6 U SIN4 SGDID:S000005180, Chr XIV from 206931-209855, Verified ORF, "Subunit of the Mediator complex; interacts with the RNA polymerase II holoenzyme to postively or negatively regulate transcription; dispensible for basal transcription" * Jamie_Ashy_SPB_108.38806.38806.2 1.2265 0.1637 12.4 2710.8323 2712.0737 3843.6 329 4.134 14.3 1 K.DLQWLEFARITPMNDDQCMLIT.T * Jamie_Ashy_SPB_43.15483.15483.4 3.0907 0.1069 17.2 4254.4565 4256.7637 8129.1 1 3.218 17.6 1 K.QYNKKWPQFYAAVQKNYADPEKDSINATALAFGYVKS.L YKR009C 2 2 6.1% 900 98703 9.0 U FOX2 SGDID:S000001717, Chr XI from 456697-453995, reverse complement, Verified ORF, "Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities" * Jamie_Ashy_SPB_66.23558.23558.2 1.6323 0.1256 15.3 2352.4521 2350.6033 5735.4 104 3.817 23.7 1 K.YTTKDCILYNLGLGCTSKEL.K * Jamie_Ashy_SPB_100.35715.35715.3 1.9 0.0396 35.7 3831.2944 3829.4783 8220.7 180 3.394 14.7 1 K.VKAWKQGSVVVFQTIDTTRNVIVLDNAAVKLSQAK.S YKL054C 2 2 6.1% 738 83973 5.0 U DEF1 SGDID:S000001537, Chr XI from 338397-336181, reverse complement, Verified ORF, "RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII" * Jamie_Ashy_SPB_25.09022.09022.3 2.4667 0.0098 47.0 1889.4543 1891.1404 5590.1 409 3.066 29.7 1 R.KSNHNSHSSKKLNPALK.S * Jamie_Ashy_SPB_37.13323.13323.2 1.7777 0.0466 10.8 3207.912 3209.3757 6546.6 42 3.822 20.4 1 T.YKSSNNSNSFTSTKHNSSNNYTQARNKK.K YLR047C 2 2 6.1% 686 78949 9.1 U FRE8 SGDID:S000004037, Chr XII from 241408-239348, reverse complement, Verified ORF, "Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p" * Jamie_Ashy_SPB_67.23862.23862.2 1.6119 0.0814 13.6 1755.8522 1755.0913 6618.9 27 3.754 34.4 1 R.VFMCVGGSGISFGLPLL.R * Jamie_Ashy_SPB_23.08173.08173.4 2.9792 0.093 15.2 2876.9365 2874.049 6187.0 332 3.387 21.5 1 R.NHHPPKTSDIPDINADDEIDFTYAF.S YBR060C 2 2 6.1% 620 71238 9.4 U ORC2 SGDID:S000000264, Chr II from 362512-360650, reverse complement, Verified ORF, "Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p" * Jamie_Ashy_SPB_57.20432.20432.2 1.4594 0.1459 17.1 2572.3323 2575.036 6802.2 3 3.766 26.2 1 K.PKTPSKAPRKRGRPRKIQEELT.D * Jamie_Ashy_SPB_11.03729.03729.4 2.8835 0.1432 10.8 1816.7366 1810.9739 3933.2 1 3.924 36.7 1 N.EESKTSNNKQVMEKTG.I YBL020W 2 2 6.1% 574 66211 9.2 U RFT1 SGDID:S000000116, Chr II from 182404-184128, Verified ORF, "Flippase, essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane; mutation is suppressed by expression human p53 protein" * Jamie_Ashy_SPB_30.10603.10603.2 1.5405 0.1074 12.2 2906.0923 2908.496 7239.3 341 3.34 19.6 1 R.WSIFLIWLSIIVELLSEPFFIVNQ.F * Jamie_Ashy_SPB_53.18835.18835.2 1.7394 0.0074 69.0 1320.4521 1319.6334 6297.0 12 3.391 55.0 1 K.PKKLFSTRLTK.I YLR419W 3 3 6.0% 1435 163044 9.1 U YLR419W SGDID:S000004411, Chr XII from 958424-962731, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_5.01606.01606.4 1.725 0.0821 38.7 4322.4565 4324.8574 6476.6 354 3.376 11.0 1 R.ISAIGLAERVSDERCVTCGEEVGYVIRGVNKTKASTRIK.F * Jamie_Ashy_SPB_46.16465.16465.3 1.8038 0.0078 33.4 3428.8145 3431.978 8284.8 426 2.753 17.9 1 R.AKSMFYNPKDNTTKLIESFISKAEVKQRR.G * Jamie_Ashy_SPB_30.10688.10688.2 1.8246 0.0524 12.9 2032.3722 2033.3806 6338.4 59 3.344 32.4 1 R.NFDILRAILTGAFYPHIA.R Reverse_YML065W 3 3 6.0% 914 104400 5.7 U ORC1 SGDID:S000004530, Chr XIII from 142210-144954, Verified ORF, "Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; may be phosphorylated by Cdc28p" * Jamie_Ashy_SPB_24.08617.08617.4 2.8823 0.109 17.5 2237.1765 2233.6133 3468.4 103 3.644 28.6 1 K.VVERVTLTKGVGPTGAVYITTA.S * Jamie_Ashy_SPB_32.11409.11409.2 1.8891 0.0975 16.3 1713.3322 1712.092 5552.1 202 3.261 38.5 1 A.KDKKQPGRKIEKKK.S * Jamie_Ashy_SPB_27.09537.09537.2 1.5438 0.1198 12.7 2386.1921 2386.6316 8738.2 373 3.33 25.0 1 K.NYYNLPRNLINADPNFQRY.H YDR303C 2 2 6.0% 885 101720 8.0 U RSC3 SGDID:S000002711, Chr IV from 1071380-1068723, reverse complement, Verified ORF, "One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p" * Jamie_Ashy_SPB_75.26691.26691.3 2.6006 0.1189 12.1 2706.8643 2710.8977 8135.2 31 3.941 23.9 1 N.KMDCFYPDVPGQYVPSSSSSSNTR.Q * Jamie_Ashy_SPB_58.20685.20685.4 2.9691 0.1649 10.7 3178.0566 3174.473 5411.4 99 3.981 20.2 1 I.TGKKITGVNLDLKQDSSAQMQSSHSNRNQ.E YBL023C 3 3 6.0% 868 98779 5.1 U MCM2 SGDID:S000000119, Chr II from 177529-174923, reverse complement, Verified ORF, "Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex" * Jamie_Ashy_SPB_64.23007.23007.4 3.1245 0.0804 81.4 3665.0166 3669.1782 4993.7 300 3.034 18.9 1 R.IHSEIHVRISDFPTIYSLRELRESNLSSLVR.V * Jamie_Ashy_SPB_12.04257.04257.2 1.9726 0.0226 14.1 1221.8322 1221.4049 4954.6 108 3.373 56.2 1 E.EREFRKISR.D * Jamie_Ashy_SPB_32.11216.11216.2 1.5737 0.1278 15.5 1143.5122 1141.2723 5399.8 140 3.584 45.5 1 C.SIIAAANPNGGR.Y Reverse_YPL029W 2 2 6.0% 737 84324 9.0 U SUV3 SGDID:S000005950, Chr XVI from 495504-497717, Verified ORF, "ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Msu1p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs" * Jamie_Ashy_SPB_13.04485.04485.2 1.765 0.1317 17.1 2240.112 2237.579 3475.7 31 3.874 38.2 1 L.RYSLLGRTHRKAITECFK.T * Jamie_Ashy_SPB_31.11054.11054.4 3.3801 0.0381 77.1 2879.6565 2875.3477 5002.4 176 3.169 22.7 1 K.SFAVVCDGKRLGKIGDKIPKEEVSLK.G Reverse_YBL034C 4 4 5.9% 1513 174176 6.3 U STU1 SGDID:S000000130, Chr II from 158392-153851, reverse complement, Verified ORF, "Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles" * Jamie_Ashy_SPB_31.11017.11017.3 2.2436 0.0136 39.3 2401.4644 2400.903 5740.1 26 2.533 27.6 1 R.PFNDMILLHMKESKKNNVLK.M * Jamie_Ashy_SPB_76.27402.27402.2 1.3354 0.1294 11.1 2652.412 2653.075 8198.6 87 3.582 26.2 1 K.ELEHCIKMKTSVIDFDALLKNF.R * Jamie_Ashy_SPB_63.22604.22604.2 1.5473 0.0842 10.8 2313.9922 2313.7 6098.9 7 3.065 26.1 1 K.LKNSLKSAIVSGGATVAPANLLST.S * Jamie_Ashy_SPB_49.17469.17469.2 1.5692 0.0897 13.2 2345.8523 2346.3892 10140.4 94 3.353 22.7 1 K.RSSNNSSHSYLRSTASSASATSS.V Reverse_YLR278C 4 4 5.9% 1341 151278 8.3 U YLR278C SGDID:S000004268, Chr XII from 704026-700001, reverse complement, Uncharacterized ORF, "Protein of unknown function, localizes to the nucleus; potential Cdc28p substrate" * Jamie_Ashy_SPB_14.04924.04924.3 2.7702 0.1276 16.5 2275.4644 2275.3506 5654.2 20 3.387 26.1 1 N.SNSLNAANASNSDHLTDGSSIAK.N * Jamie_Ashy_SPB_76.27168.27168.2 1.6901 0.1023 17.1 2505.0923 2504.811 8636.5 1 4.642 34.1 1 R.RLKLSQNIFQVGHDNTSPAAAHV.G * Jamie_Ashy_SPB_15.05217.05217.2 1.807 0.0992 15.2 1694.5322 1695.0271 6293.7 6 3.337 46.2 1 G.MVDKIIDYLILSDR.D * Jamie_Ashy_SPB_80.28635.28635.2 1.4116 0.0212 62.1 2020.5922 2023.1234 8456.9 405 2.679 25.0 1 K.RFENADGADVNSNSTSIPK.I YPR010C 2 2 5.9% 1203 135742 7.7 U RPA135 SGDID:S000006214, Chr XVI from 581193-577582, reverse complement, Verified ORF, "RNA polymerase I subunit A135" * Jamie_Ashy_SPB_79.28376.28376.4 2.9752 0.1122 17.3 3426.4565 3424.8079 6192.6 72 3.314 18.8 1 S.NSGYLGNKLSVSVEQVSIAKPMSNDGVSSAVER.K * Jamie_Ashy_SPB_44.15644.15644.4 3.023 0.0413 71.1 4658.1367 4661.52 7887.8 353 2.996 14.9 1 K.EKIDEYLQNIIAQVRMDINRGMAINFKDKRYMSRVLMR.V Reverse_YJL080C 3 3 5.9% 1222 134809 5.8 U SCP160 SGDID:S000003616, Chr X from 289142-285474, reverse complement, Verified ORF, "Essential RNA-binding G protein effector of mating response pathway, predominantly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins" * Jamie_Ashy_SPB_54.19318.19318.3 1.4602 0.0843 33.6 4952.7544 4954.6323 7077.3 121 3.152 11.0 1 R.IIENLIKKEAKEVNEPAGTITIKGSPDDKNPVFLNINFESELQR.R * Jamie_Ashy_SPB_12.04310.04310.4 2.1325 0.0065 38.5 1701.9365 1700.9323 5822.7 310 2.498 29.8 1 K.VNPHSEELGKLVSYK.T * Jamie_Ashy_SPB_62.22239.22239.2 1.7229 0.0597 13.5 1467.6122 1467.7056 4824.1 355 3.246 41.7 1 K.KLYDQISTKTAKA.K YJR132W 3 3 5.9% 1048 119983 4.7 U NMD5 SGDID:S000003893, Chr X from 669437-672583, Verified ORF, "Karyopherin, a carrier protein involved in nuclear import of proteins; importin beta homolog" * Jamie_Ashy_SPB_27.09450.09450.3 2.6511 0.1294 17.1 2335.0144 2338.4492 4229.7 5 3.639 30.3 1 K.PTAETLEAFDNDPQEYINRN.M * Jamie_Ashy_SPB_85.30326.30326.4 3.0 0.1701 17.2 3232.6565 3229.644 5315.3 9 3.938 22.2 1 I.NRNMDFWDVGYSPDLAALALLTTCVTKR.G * Jamie_Ashy_SPB_76.27336.27336.2 1.6424 0.0694 11.0 2104.0923 2105.3545 7211.0 152 3.155 28.1 1 F.YPAAEFILKNDIEDFYR.E Reverse_YOL063C 2 2 5.9% 957 109715 5.7 U YOL063C SGDID:S000005424, Chr XV from 210264-207391, reverse complement, Uncharacterized ORF, "Protein required for normal hydroxyurea resistance; not related to the HUS1 checkpoint protein identified in human and fission yeast" * Jamie_Ashy_SPB_98.35154.35154.4 2.7984 0.1248 87.0 4684.0566 4686.869 10910.7 301 3.689 13.8 1 R.QFSTLSSGSEENEPDSNVDDEETSNLQLSCIEPDKLVTNLDK.G * Jamie_Ashy_SPB_17.05852.05852.2 1.6291 0.0923 12.4 1569.6721 1570.7856 3710.8 34 3.267 38.5 1 R.FNRVDGDDPIIVPL.L Reverse_YHR120W 2 2 5.9% 959 109408 8.7 U MSH1 SGDID:S000001162, Chr VIII from 349577-352456, Verified ORF, "DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis" * Jamie_Ashy_SPB_75.26968.26968.2 1.5486 0.0889 11.6 1861.1721 1864.1272 7712.7 148 3.269 31.2 1 L.ATSTRSDIQMIATALQR.Q * Jamie_Ashy_SPB_82.29486.29486.4 2.7266 0.0697 71.6 4586.8164 4590.2744 7979.7 116 3.352 14.1 1 K.KFQEAITVSYGCNNVLMKLHRSLQHVPFGAFPVKGHSYAR.N Reverse_YIL130W 2 2 5.9% 964 108781 6.2 U YIL130W SGDID:S000001392, Chr IX from 102782-105676, Uncharacterized ORF, "Proposed transcriptional activator, member of the Gal4p family of zinc cluster proteins" * Jamie_Ashy_SPB_14.04902.04902.4 2.668 0.1718 14.8 2010.0565 2008.1088 5666.6 47 4.231 32.4 1 I.FVDDTADLINSQGNNDLR.I * Jamie_Ashy_SPB_69.24582.24582.4 2.9714 0.0845 11.4 4446.6567 4450.19 6919.7 124 3.173 14.9 1 R.LASRMAVGIYTYCTSLRASCQLFIILMLIAQISNLDRAN.T Reverse_YML076C 2 2 5.9% 944 107560 7.6 U WAR1 SGDID:S000004541, Chr XIII from 115347-112513, reverse complement, Verified ORF, "Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively" * Jamie_Ashy_SPB_90.32372.32372.2 2.1522 0.1363 32.6 2059.872 2061.2654 6432.2 62 3.888 34.4 1 K.KTEKVLDECAKETVHSW.R * Jamie_Ashy_SPB_106.38190.38190.3 2.1019 0.2465 12.8 4661.574 4662.5596 8092.9 186 4.257 13.2 1 I.VSFLIPKKKRLEDVSVNEEVKIFPLKSLYSIETKYLHLR.L YOR211C 2 2 5.9% 902 101523 8.0 U MGM1 SGDID:S000005737, Chr XV from 741632-738924, reverse complement, Verified ORF, "Mitochondrial GTPase related to dynamin, present in a complex containing Ugo1p and Fzo1p; required for normal morphology of cristae and for stability of Tim11p; homolog of human OPA1 involved in autosomal dominant optic atrophy" * Jamie_Ashy_SPB_44.15646.15646.4 2.9507 0.1165 16.2 2660.8167 2654.8274 4801.0 204 3.369 23.6 1 K.EKFNKMFSGDKSQDGGHGNDGTVPT.A * Jamie_Ashy_SPB_50.17923.17923.3 2.8854 0.1021 15.1 3314.4243 3314.4849 7069.6 137 3.651 19.4 1 D.DDEDDENDSVDTTQDEMLNLTKQMIEIR.T YDR096W 2 2 5.9% 894 99481 7.5 U GIS1 SGDID:S000002503, Chr IV from 637134-639818, Verified ORF, "Transcriptional factor, involved in the expression of genes during nutrient limitation; also involved in the negative regulation of DPP1 and PHR1" * Jamie_Ashy_SPB_32.11314.11314.2 1.7896 0.0689 12.6 1958.5322 1957.2773 7978.0 401 3.24 27.8 1 P.LNSGLAPLSPSTSNIPFLK.R * Jamie_Ashy_SPB_97.34869.34869.4 3.0436 0.0823 13.1 3714.8164 3720.1367 6335.1 154 3.271 17.7 1 K.RNNVVTLNISREASKSPISSFVNDYRSPLGVSNP.L YPR029C 3 3 5.9% 832 93624 5.5 U APL4 SGDID:S000006233, Chr XVI from 626964-624466, reverse complement, Verified ORF, "Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport" * Jamie_Ashy_SPB_2.00653.00653.2 1.5008 0.0543 10.6 853.39215 853.9993 3646.0 7 3.397 80.0 1 L.RDFFIR.L * Jamie_Ashy_SPB_28.09910.09910.4 2.9281 0.1522 10.3 2932.6565 2928.4941 6027.9 16 3.459 20.8 1 I.INYILTAALKLSVRFHDAKNIEKLR.Q * Jamie_Ashy_SPB_43.15224.15224.2 2.056 0.0631 15.3 2031.4521 2031.4594 5358.5 38 3.487 32.4 1 I.CKQSLKISGSGKLKLRVK.L Reverse_YGL086W 3 3 5.9% 749 87651 5.4 U MAD1 SGDID:S000003054, Chr VII from 347122-349371, Verified ORF, "Coiled-coil protein involved in the spindle-assembly checkpoint; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of the activity of the anaphase promoting complex; forms a complex with Mad2p" * Jamie_Ashy_SPB_40.14418.14418.2 1.7061 0.0817 14.5 1243.8522 1245.4215 6353.9 7 3.186 65.0 1 Y.NLGIQDSTLKR.K * Jamie_Ashy_SPB_27.09441.09441.2 1.8054 0.0309 10.8 1455.4122 1454.7562 5743.2 473 2.899 50.0 1 N.LLKLNNNDLRLK.D * Jamie_Ashy_SPB_25.09004.09004.3 2.5944 0.2488 97.6 2640.6243 2640.0205 6553.6 6 4.0 27.5 1 K.KTLKWDRIIEEPNNRINNKTR.N YLR069C 2 2 5.9% 761 84574 6.9 U MEF1 SGDID:S000004059, Chr XII from 273916-271631, reverse complement, Verified ORF, "Mitochondrial elongation factor involved in translational elongation" * Jamie_Ashy_SPB_9.03182.03182.3 2.1488 0.1474 15.7 1541.8744 1537.7644 5829.7 14 3.641 41.7 1 R.IPFFTCSKVFSGF.S * Jamie_Ashy_SPB_108.38570.38570.4 2.6814 0.1962 10.6 3549.2966 3551.139 4921.4 108 3.685 17.7 1 V.VDLINRVAIYNKGDNGEIIEKGPVPENLKPLM.E Reverse_YFL047W 2 2 5.9% 714 82209 5.8 U RGD2 SGDID:S000001847, Chr VI from 40421-42565, Verified ORF, "GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p" * Jamie_Ashy_SPB_51.18180.18180.4 2.6604 0.0584 66.8 3113.2166 3109.5645 6522.2 249 2.806 21.7 1 K.FEELQRCKNFYEKELKGVYKKSKR.F * Jamie_Ashy_SPB_13.04634.04634.4 2.7571 0.0116 56.0 2259.5366 2258.4707 7221.3 42 3.416 28.4 1 R.SAFLQIFLDCQECGHYLR.D Reverse_YPR024W 2 2 5.9% 747 81772 7.1 U YME1 SGDID:S000006228, Chr XVI from 610478-612721, Verified ORF, "Subunit, with Mgr1p, of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; mutation causes an elevated rate of mitochondrial turnover" * Jamie_Ashy_SPB_56.20204.20204.2 1.568 0.1134 12.1 2452.2322 2450.7568 6343.1 1 4.139 35.0 1 E.HADLTEYEILGQALRHLEVNK.K * Jamie_Ashy_SPB_42.15162.15162.2 1.4984 0.1071 12.5 2457.6921 2457.6567 6846.1 34 4.01 22.7 1 Q.KTKDNTNSPPPPQSNDISSITSK.N Reverse_YIL037C 2 2 5.9% 656 75021 8.0 U PRM2 SGDID:S000001299, Chr IX from 284998-283028, reverse complement, Verified ORF, "Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p" * Jamie_Ashy_SPB_81.29134.29134.2 1.6969 0.1854 29.9 2541.892 2540.9692 8497.5 4 4.617 26.1 1 K.IQRKSLSSLAAVSANTINIGRNII.G * Jamie_Ashy_SPB_20.07033.07033.2 2.0142 0.0249 78.7 1696.1921 1694.8976 6346.0 3 3.624 46.4 1 K.EQMDTGVTSEISRLK.S Reverse_YPR111W 2 2 5.9% 564 65880 8.5 U DBF20 SGDID:S000006315, Chr XVI from 747304-748998, Verified ORF, "Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis" Jamie_Ashy_SPB_24.08373.08373.2 1.5431 0.147 16.4 1027.5922 1025.2048 4464.0 58 3.663 56.2 1 K.GELVELAMY.D * Jamie_Ashy_SPB_116.41512.41512.2 1.3789 0.209 18.8 2653.4521 2654.0042 5332.1 44 4.023 19.6 1 P.LGPTLRGNESKRAPRYQAQPSTPK.P YBR293W 2 2 5.9% 474 51677 9.7 U VBA2 SGDID:S000000497, Chr II from 787001-788425, Verified ORF, "Permease of basic amino acids in the vacuolar membrane" * Jamie_Ashy_SPB_48.17036.17036.4 2.6473 0.0811 74.4 2636.5764 2640.2068 4741.2 25 3.488 25.4 1 R.AHLMSTLLSSLALILCILKDNLAK.P * Jamie_Ashy_SPB_46.16435.16435.2 2.5619 0.161 69.0 1896.9321 1897.3214 6866.0 1 4.066 46.7 1 A.LILCILKDNLAKPKTR.R YJL046W 2 2 5.9% 409 47003 7.4 U YJL046W SGDID:S000003582, Chr X from 352302-353531, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_101.36399.36399.3 2.4555 0.1842 13.1 2501.4844 2497.813 5273.6 322 3.906 22.8 1 R.GDIIQDGFKISGSAYKIAGGKAYH.H * Jamie_Ashy_SPB_46.16303.16303.2 1.9785 0.1772 46.2 1522.8922 1523.7318 5543.0 156 4.215 42.9 1 K.ISGSAYKIAGGKAYH.H YLR430W 5 5 5.8% 2231 252495 8.6 U SEN1 SGDID:S000004422, Chr XII from 993430-1000125, Verified ORF, "Nuclear protein, putative helicase required for processing of tRNAs, rRNAs, and small nuclear RNAs; potential Cdc28p substrate" * Jamie_Ashy_SPB_71.25264.25264.4 3.0063 0.0875 14.7 3863.3364 3868.5056 7125.0 104 3.239 17.2 1 E.VLLESCVRLIISTSDLAHEKHVKVDDSLVEMMAK.Y * Jamie_Ashy_SPB_38.13639.13639.2 1.7049 0.1056 16.2 1656.3121 1658.9033 8044.8 37 3.146 39.3 1 N.NRVVHPPSAPAFHTK.S * Jamie_Ashy_SPB_104.37249.37249.4 1.705 0.0612 35.2 5683.4966 5678.4863 4630.0 315 3.627 8.2 1 K.VMQMTTIEREYSTLEGLEYYDLVGQILQAKPSPPVNVDAAEIETVKKSYK.L * Jamie_Ashy_SPB_29.10260.10260.2 2.1421 0.2127 72.2 1711.4922 1712.8999 7748.9 25 4.16 42.3 1 M.EKNSSPYLLDVQYR.M * Jamie_Ashy_SPB_69.24835.24835.2 2.1601 0.1171 29.2 1970.4122 1973.2454 7118.0 14 4.342 34.4 1 C.SGFLDPRNNRAQSILRK.F YDR141C 4 4 5.8% 1698 194686 5.7 U DOP1 SGDID:S000002548, Chr IV from 739992-734896, reverse complement, Verified ORF, "Protein of unknown function, essential for viability, involved in establishing cellular polarity and morphogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_Ashy_SPB_43.15337.15337.2 1.9347 0.0451 13.3 1547.3121 1549.8564 5695.5 477 3.399 38.5 1 K.FPSLNAVPHLVNKI.K * Jamie_Ashy_SPB_29.10256.10256.4 2.9452 0.1493 11.6 2964.8564 2959.5046 5117.2 154 3.253 20.8 1 K.LQKFSLYNKLLNYIPERALLPLSHS.K * Jamie_Ashy_SPB_82.29298.29298.4 3.4186 0.1031 12.3 4386.8965 4387.8164 7805.4 6 3.53 18.0 1 K.IPESEELKGRSHISWSDKKITQSIFEAIPRLCESNNDA.K * Jamie_Ashy_SPB_52.18410.18410.2 1.4184 0.0386 64.3 2560.1921 2562.7148 7623.1 14 3.21 26.2 1 K.SNDDSSLDATNHNSYKFKFRSK.K YGR281W 3 3 5.8% 1477 166727 7.7 U YOR1 SGDID:S000003513, Chr VII from 1052830-1057263, Verified ORF, "Plasma membrane transporter of the ATP-binding cassette (ABC) family, mediates export of many different organic anions including oligomycin" * Jamie_Ashy_SPB_23.08156.08156.4 3.6411 0.0351 12.3 3398.7366 3396.9165 6935.8 53 3.496 20.8 1 A.LPLYAILVVGTTFCSLFSSVWLSYWTENK.F * Jamie_Ashy_SPB_11.04002.04002.2 2.2316 0.156 60.7 1361.1122 1361.584 5052.4 2 3.993 63.6 1 M.NEAGYLVVVLQR.W * Jamie_Ashy_SPB_69.24644.24644.4 3.5598 0.2105 66.7 4887.0967 4884.4087 7363.8 1 4.315 15.5 1 V.EEEGSNFSLGERQLLALTRALVRQSKILILDEATSSVDYETDGK.I YCR033W 2 2 5.8% 1226 138397 9.1 U SNT1 SGDID:S000000629, Chr III from 186484-190164, Verified ORF, "Subunit of the Set3C deacetylase complex; putative DNA-binding protein" * Jamie_Ashy_SPB_14.05052.05052.4 2.5083 0.2056 15.1 4817.7363 4823.321 6823.7 35 4.119 14.7 1 S.TTISNLLNNSDRSMKSSFQSASRHEAQLEDTPSMNNIVVQEIK.P * Jamie_Ashy_SPB_33.11800.11800.3 2.4281 1.0E-4 39.5 2868.0244 2872.2654 6751.9 5 2.735 22.2 1 K.FNFSNDPLAALAAVASAPDAMSSFLSKK.E Reverse_YOR109W 2 2 5.8% 1107 124577 7.2 U INP53 SGDID:S000005635, Chr XV from 525278-528601, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in a TGN (trans Golgi network)-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches" * Jamie_Ashy_SPB_43.15187.15187.3 2.1739 0.0043 35.9 2703.8943 2705.1758 6088.6 8 2.917 25.0 1 K.EKSTSPAPASVSKHAPPPPPRLVPKK.I * Jamie_Ashy_SPB_49.17638.17638.4 2.9701 0.1354 12.9 4497.7764 4496.0146 9835.7 118 4.137 15.3 1 D.ELFTRFAALSIIQQALNTRDLCDLCNTRFIGHQESINK.K Reverse_YDL215C 3 3 5.8% 1092 124332 5.7 U GDH2 SGDID:S000002374, Chr IV from 73919-70641, reverse complement, Verified ORF, "NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels" * Jamie_Ashy_SPB_19.06828.06828.2 2.3926 0.0423 10.2 1451.4521 1452.6042 3861.4 7 2.96 58.3 1 L.AAEVDHLLVDELQ.Q * Jamie_Ashy_SPB_25.08738.08738.2 1.7557 0.0687 12.8 1832.2122 1831.2957 6646.0 275 3.29 35.7 1 G.EREKVLKLLLGYLKK.N * Jamie_Ashy_SPB_22.07933.07933.4 2.9521 0.0877 11.1 3776.3364 3782.1155 8607.1 124 3.016 17.6 1 R.NKKITDKGHELTDNGVADSAFKFNKQQSDSISVGT.N Reverse_YPR055W 2 2 5.8% 1065 122224 6.8 U SEC8 SGDID:S000006259, Chr XVI from 667673-670870, Verified ORF, "Essential 121kDa subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, and Exo84p), which has the essential function of mediating polarized targeting of secretory vesicles to active sites of exocytosis" * Jamie_Ashy_SPB_116.41498.41498.4 2.3067 0.1588 15.0 4926.7363 4922.6074 6651.1 7 3.77 13.5 1 D.MNSSVSFGPFIDKLLAKLEFAQDKVSSNDEINYELSFIKPNKR.K * Jamie_Ashy_SPB_72.25826.25826.2 1.7964 0.0867 17.6 2295.612 2295.6152 9317.0 2 3.704 33.3 1 K.NITKSHEMIDINVINYLYN.E YEL055C 2 2 5.8% 1022 115894 5.3 U POL5 SGDID:S000000781, Chr V from 51539-48471, reverse complement, Verified ORF, "Protein with sequence similarity to the human MybBP1A and weak sequence similar to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA" * Jamie_Ashy_SPB_47.16909.16909.2 1.7286 0.1012 16.3 2509.172 2506.815 6961.8 50 4.115 25.0 1 R.LHAAVALIKDLSALDLPDDAEEW.S * Jamie_Ashy_SPB_117.42142.42142.4 2.8729 0.1598 11.2 4262.2563 4264.951 6670.9 87 3.891 16.2 1 K.LFGLKSLLNEPLFSEIFVKDLEKGNTEFFIRFTEQL.I Reverse_YOR219C 2 2 5.8% 931 107200 6.0 U STE13 SGDID:S000005745, Chr XV from 755009-752214, reverse complement, Verified ORF, "Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor" * Jamie_Ashy_SPB_59.21277.21277.2 1.7137 0.0945 14.8 2271.5923 2272.606 8305.2 31 3.314 27.5 1 S.SKTTFTQSGPGGYINVLIPYK.K * Jamie_Ashy_SPB_83.29614.29614.4 2.7892 0.1079 15.1 3592.2566 3595.749 6685.9 224 3.035 18.2 1 E.NITHGNENSQPDTNAVSDNNPFFPKDMSSNSSK.R Reverse_YGL049C 2 2 5.8% 914 103899 7.6 U TIF4632 SGDID:S000003017, Chr VII from 409607-406863, reverse complement, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4631p" * Jamie_Ashy_SPB_88.31390.31390.4 2.899 0.0463 67.7 3797.0566 3802.187 6325.7 383 2.9 17.1 1 K.VAENAPLVPTAEKEFPTGSKSKQEVGDNKEEGKSAK.K * Jamie_Ashy_SPB_17.05941.05941.2 2.134 0.09 20.1 1943.1322 1944.0305 5984.5 35 3.408 37.5 1 Q.ANGQSNNRNNYKNTGYK.G YNL221C 2 2 5.8% 875 100395 9.8 U POP1 SGDID:S000005165, Chr XIV from 233696-231069, reverse complement, Verified ORF, "Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in Rnase P" * Jamie_Ashy_SPB_118.42409.42409.2 1.8969 0.0957 17.3 1557.8522 1558.7312 10375.9 14 4.06 42.3 1 K.DIHNTKLPAYSGEI.G * Jamie_Ashy_SPB_64.22915.22915.4 3.3396 0.0912 17.3 3986.9365 3989.5066 7491.4 281 3.279 15.7 1 K.IPLPEVHDLIGFITSGTYHLNCGNGMGIGFIDHHAAI.R YLR386W 2 2 5.8% 880 99773 5.5 U VAC14 SGDID:S000004378, Chr XII from 893628-896270, Verified ORF, "Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; activator of Fab1p" * Jamie_Ashy_SPB_45.16050.16050.2 1.9172 0.1199 20.2 2524.2922 2524.8826 4707.5 493 4.291 23.9 1 I.VAERASNYISIVNNGSHGLLPAIK.T * Jamie_Ashy_SPB_96.34309.34309.3 2.2081 0.0257 39.3 3170.9043 3168.7375 7431.6 88 3.225 20.2 1 R.TVTHTLMDSLLHEVDRISKLQTEIKMK.R Reverse_YBL067C 2 2 5.8% 747 83865 6.2 U UBP13 SGDID:S000000163, Chr II from 95884-93641, reverse complement, Verified ORF, "Putative ubiquitin-specific protease" Jamie_Ashy_SPB_8.02690.02690.2 1.6995 0.0576 85.7 799.7322 799.9053 3520.6 15 3.016 75.0 1 D.QPRSSPK.G * Jamie_Ashy_SPB_48.17082.17082.4 3.0317 0.1042 17.5 3851.0964 3851.2676 7070.3 419 3.248 17.1 1 K.FFKSKKESPGATNSNVSNVPTPKGDDVSQPKPNPLH.G Reverse_YCR091W 2 2 5.8% 720 81494 8.5 U KIN82 SGDID:S000000687, Chr III from 274400-276562, Verified ORF, "Putative serine/threonine protein kinase, most similar to cyclic nucleotide-dependent protein kinase subfamily and the protein kinase C subfamily" * Jamie_Ashy_SPB_52.18723.18723.2 1.5126 0.1068 11.6 2534.412 2535.859 6931.0 108 4.049 25.0 1 T.AKYLIKGYTYNESYTLINHDK.K * Jamie_Ashy_SPB_7.02383.02383.4 2.7622 0.1118 19.5 2529.6565 2525.935 3820.5 255 3.478 26.7 1 A.ARRLRPTINMPIQDKRTESNK.R YGR077C 2 2 5.8% 589 68165 7.6 U PEX8 SGDID:S000003309, Chr VII from 639517-637748, reverse complement, Verified ORF, "Intraperoxisomal organizer of the peroxisomal import machinery, tightly associated with the lumenal face of the peroxisomal membrane, essential for peroxisome biogenesis, binds PTS1-signal receptor Pex5p" * Jamie_Ashy_SPB_58.20817.20817.4 3.2049 0.075 15.5 3908.6165 3902.671 7207.1 222 3.188 16.7 1 K.LQLFSPIWAQLWNTAIVRANLSIQHCLLIALALL.F * Jamie_Ashy_SPB_57.20468.20468.4 3.0572 0.1578 10.8 2668.0566 2671.1172 6005.1 127 3.642 27.3 1 L.QLFSPIWAQLWNTAIVRANLSIQ.H Reverse_YHR184W 2 2 5.8% 571 65816 5.8 U SSP1 SGDID:S000001227, Chr VIII from 472744-474459, Verified ORF, "Protein involved in the control of meiotic nuclear division and coordination of meiosis with spore formation; transcription is induced midway through meiosis" * Jamie_Ashy_SPB_24.08550.08550.4 2.9408 0.1289 15.2 2687.4165 2686.0813 3589.0 61 3.694 21.5 1 L.AAALVKSEESASEPKVQTKKISLNR.L * Jamie_Ashy_SPB_41.14778.14778.2 1.7776 0.1218 17.5 1499.5922 1499.7538 5586.5 270 3.599 38.5 1 K.KISLNRLSAANVNV.N Reverse_YJL111W 2 2 5.8% 550 59736 5.5 U CCT7 SGDID:S000003647, Chr X from 207794-209446, Verified ORF, "Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo" * Jamie_Ashy_SPB_51.18235.18235.2 1.8588 0.0259 73.7 1865.2722 1864.1738 8261.3 46 3.467 32.4 1 R.LCKSVEMETAGGGAVILK.N * Jamie_Ashy_SPB_81.28998.28998.2 1.819 0.0845 15.6 1474.2122 1475.7257 4467.4 48 3.707 42.3 1 L.LKLITAGDNSITTK.Q YBR136W 5 5 5.7% 2368 273340 8.3 U MEC1 SGDID:S000000340, Chr II from 505662-512768, Verified ORF, "Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; monitors and participates in meiotic recombination" * Jamie_Ashy_SPB_57.20310.20310.4 4.0203 0.1799 65.9 4195.6567 4194.678 8475.9 116 3.948 16.2 1 R.LTSWTVILGRLACLESEKFSGTLPNSTKDMDNWYVC.H * Jamie_Ashy_SPB_32.11270.11270.4 3.365 0.1225 10.2 2604.4565 2599.9873 5976.0 128 3.472 24.2 1 D.DREVLLQYNIAKALIAISNEDPL.R * Jamie_Ashy_SPB_45.15946.15946.3 2.3547 0.0029 39.0 3279.5645 3275.6555 6109.1 422 3.118 17.0 1 R.QHSQNPHDLVSSALDLTKALTRVCLQDVK.S * Jamie_Ashy_SPB_18.06387.06387.4 3.4409 0.0714 17.9 3382.3364 3379.8738 9221.1 197 3.088 19.6 1 R.QDNQYMQFATTMDFLLSKDIASRKRSLGI.N * Jamie_Ashy_SPB_14.04797.04797.2 1.9219 0.0052 70.8 2164.872 2165.6084 6894.1 1 2.648 44.1 1 R.NMDHSIQKALKVLRNKIR.G Reverse_YDR127W 3 3 5.7% 1588 174754 6.3 U ARO1 SGDID:S000002534, Chr IV from 704479-709245, Verified ORF, "Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids" * Jamie_Ashy_SPB_21.07284.07284.3 2.0727 0.2109 16.1 3234.2944 3234.7576 5259.4 8 4.454 21.3 1 Q.IDTPLTLELDLFEVGNKLAIDYLERLTK.F * Jamie_Ashy_SPB_53.18867.18867.3 2.6231 0.1431 15.3 2107.9443 2103.575 4601.8 10 4.237 31.6 1 S.ITTKGAARMGIIVVSKKSNK.K * Jamie_Ashy_SPB_73.26110.26110.4 2.7262 0.1282 18.8 4512.177 4507.252 10966.4 9 3.266 16.3 1 Y.LKSIPKGGVLALTVPEEAYPACMLISSVYQSSVTAALEIRGGK.F YIL026C 2 2 5.7% 1150 133009 5.1 U IRR1 SGDID:S000001288, Chr IX from 307927-304475, reverse complement, Verified ORF, "Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability" * Jamie_Ashy_SPB_77.27625.27625.4 3.3807 0.1007 12.4 3788.5764 3786.0671 6449.3 303 3.501 18.2 1 Y.EDQDDDDYVDTATAKRKSRKRKPKSASNTSSKR.Q * Jamie_Ashy_SPB_61.21754.21754.4 3.4539 0.1022 13.8 3854.1765 3852.6575 7279.6 48 3.333 17.7 1 N.IETEFLLFTVKLKGLMKLNILDERFASRVALNK.E YKL064W 2 2 5.7% 969 109736 7.5 U MNR2 SGDID:S000001547, Chr XI from 317408-320317, Verified ORF, "Putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations" * Jamie_Ashy_SPB_88.31435.31435.4 2.8372 0.1212 18.5 4598.4966 4603.9087 6269.4 169 3.904 15.0 1 Y.DEDEEDGTSNEDGILFSKPIVTNIDVPELGNRRVNETENLK.N * Jamie_Ashy_SPB_20.07166.07166.3 2.3285 0.1217 14.8 1504.0443 1500.6952 4095.5 6 3.254 36.5 1 K.SRIQSGLQDNLLVG.R Reverse_YDR285W 3 3 5.7% 875 100035 6.8 U ZIP1 SGDID:S000002693, Chr IV from 1032430-1035057, Verified ORF, "Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate" * Jamie_Ashy_SPB_9.03086.03086.2 1.4232 0.0538 63.6 1829.4122 1832.181 5845.6 13 3.358 36.7 1 K.RSSIPPKMCNSGKKIK.G * Jamie_Ashy_SPB_86.30870.30870.3 1.7997 0.052 35.7 4044.3843 4044.5662 7357.6 16 3.132 17.4 1 K.NKGLDNITKNLNEISTKFGSIRRQYLQHNQRLDK.L * Jamie_Ashy_SPB_7.02365.02365.2 1.4882 0.0972 11.7 1136.3522 1138.3091 6454.6 118 3.101 61.1 1 N.EISTKFGSIR.R Reverse_YDR385W 2 2 5.7% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" Reverse_YOR133W 2 2 5.7% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" Jamie_Ashy_SPB_18.06446.06446.4 3.0281 0.0749 12.2 2550.5764 2553.0117 5552.7 189 3.071 24.6 1 L.DKEDGKLVIELKELLVPIEDKK.F Jamie_Ashy_SPB_98.35062.35062.2 1.4163 0.1696 15.5 2911.1921 2910.3826 5967.3 10 3.897 22.0 1 T.LTSKGHDVHAIVSMNRVNTVKDMLSR.M YLL034C 3 3 5.7% 837 93069 5.4 U RIX7 SGDID:S000003957, Chr XII from 73145-70632, reverse complement, Verified ORF, "Putative ATPase of the AAA family, required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions" * Jamie_Ashy_SPB_29.10221.10221.2 1.6458 0.0448 73.5 1207.6921 1207.3262 5428.4 454 2.975 55.0 1 K.KNSLTSSLDNK.I * Jamie_Ashy_SPB_75.26830.26830.2 1.6604 0.1319 89.5 1552.8322 1552.589 5716.8 210 3.378 35.7 1 K.SGSVSESITETDDPK.T * Jamie_Ashy_SPB_21.07515.07515.3 2.8626 0.1053 19.6 2307.5344 2310.671 4965.6 302 3.408 25.0 1 P.FISISAPSVVSGMSGESEKKIR.D YER077C 2 2 5.7% 688 79548 9.4 U YER077C SGDID:S000000879, Chr V from 316596-314530, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_50.17913.17913.3 1.8683 0.0175 33.6 2135.4543 2139.4165 6065.9 179 3.114 27.6 1 R.QSLKAVINKDASNAEATLHK.I * Jamie_Ashy_SPB_50.18043.18043.3 2.2056 0.018 38.8 3748.8843 3750.2034 6050.9 115 3.171 16.2 1 K.AVINKDASNAEATLHKIRGFIDSVEATYSSKLNGK.V YLL058W 2 2 5.7% 575 64223 7.0 U YLL058W SGDID:S000003981, Chr XII from 23569-25296, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_91.32580.32580.2 1.4377 0.0297 63.7 1818.8121 1819.1196 8117.5 77 2.989 35.7 1 K.GRIVENRFRPFGLEK.K * Jamie_Ashy_SPB_51.18396.18396.2 2.2557 0.2547 82.9 2090.8323 2090.5168 6480.8 175 4.106 29.4 1 D.IQWLLKVFSSALDVVKSR.G YHR097C 2 2 5.7% 366 40663 9.9 U YHR097C SGDID:S000001139, Chr VIII from 298612-298488,298363-297388, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_82.29564.29564.2 2.1114 0.1744 57.8 1520.1522 1522.6609 4922.5 289 4.647 41.7 1 P.FRVSPSEDRVSSR.D * Jamie_Ashy_SPB_1.00343.00343.1 1.6007 0.0412 86.1 972.52 973.1666 2833.8 46 3.063 64.3 1 R.RKNSIARK.K YER155C 6 6 5.6% 2167 245428 8.4 U BEM2 SGDID:S000000957, Chr V from 482843-476340, reverse complement, Verified ORF, "Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence" * Jamie_Ashy_SPB_26.09284.09284.2 1.759 0.074 12.5 1788.5322 1787.8662 6803.0 74 3.378 36.7 1 R.NSTSGASPYMHSHHHH.G * Jamie_Ashy_SPB_50.17726.17726.2 1.6564 0.123 15.3 2010.7122 2010.2108 6012.9 478 3.543 25.0 1 K.SGLNSNIKFFDPTLPASNS.S * Jamie_Ashy_SPB_91.32532.32532.4 2.6191 0.0083 44.2 4147.2563 4142.04 5993.2 487 2.944 15.7 1 R.AVINLLLMFPLLDHFIKFLKVFNQFGLSFTKNKSR.L * Jamie_Ashy_SPB_77.27594.27594.3 2.5806 0.0504 57.3 3100.1643 3101.4583 6786.5 32 3.114 22.2 1 R.LTNNSTQFYNISPAVDDSMTQRLALTAK.T * Jamie_Ashy_SPB_18.06258.06258.2 1.7852 0.1823 97.8 983.97217 983.06903 4898.1 122 4.015 62.5 1 K.AYSTIGSQR.S * Jamie_Ashy_SPB_73.26215.26215.2 2.132 0.0223 13.0 1659.4521 1659.8802 5964.7 40 3.578 46.4 1 N.NIGQVSDSLKESIIR.V Reverse_YLR086W 3 3 5.6% 1418 162188 6.2 U SMC4 SGDID:S000004076, Chr XII from 302244-306500, Verified ORF, "Subunit of the condensin complex, which reorganizes chromosomes during cell division, forms a stable complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity and promotes knotting of circular DNA; potential Cdc28p substrate" * Jamie_Ashy_SPB_77.27706.27706.2 1.937 0.1934 48.5 2315.5322 2315.6775 6248.7 6 4.539 28.9 1 Q.KLNQAVLTDRLVSYFANSFK.P * Jamie_Ashy_SPB_117.41937.41937.4 2.651 0.1349 18.2 4295.0166 4292.7437 8583.0 104 3.633 16.2 1 K.AQTEELLSLESEALQIQSEKEQLQLDWPELEKEHRI.I * Jamie_Ashy_SPB_23.08172.08172.3 4.4464 0.0928 32.3 2537.0645 2535.8079 4520.4 1 3.676 35.2 1 N.ATGIIDELYELLGDDSEKEAKPK.M Reverse_YOL078W 3 3 5.6% 1176 131378 6.7 U AVO1 SGDID:S000005438, Chr XV from 181681-185211, Verified ORF, "Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth" * Jamie_Ashy_SPB_26.09072.09072.3 2.2488 0.1015 69.6 2104.5842 2105.3157 5065.8 346 3.433 29.2 1 K.RNSSTLSTPEKGSIVTDRR.E * Jamie_Ashy_SPB_69.24824.24824.4 2.5544 0.129 15.0 2884.6165 2890.2961 5191.8 28 3.661 25.3 1 G.KKNHRAGPPHLTSSKARTNTTNMVNK.N * Jamie_Ashy_SPB_102.36460.36460.2 1.6459 0.2351 97.7 2498.2522 2496.7725 10484.1 16 4.516 30.0 1 K.KQMEMSSNLYSDEKSSTKHHK.G Reverse_YAL051W 2 2 5.6% 1047 118863 6.8 U OAF1 SGDID:S000000048, Chr I from 48565-51708, Verified ORF, "Oleate-activated transcription factor, acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis" * Jamie_Ashy_SPB_72.25646.25646.3 2.1606 0.043 42.5 4649.2144 4646.3013 9282.5 20 3.486 14.5 1 K.TVNFSTLSSIAHRYEGNFFKNFYNILSLADMCSVMFYKK.Y * Jamie_Ashy_SPB_14.05068.05068.2 1.8675 0.1946 50.0 2230.0522 2229.4985 5518.5 26 4.127 31.6 1 T.NIPSASRKGSENVERETLLK.M Reverse_YDL031W 3 3 5.6% 995 113158 9.3 U DBP10 SGDID:S000002189, Chr IV from 394214-397201, Verified ORF, "Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis" * Jamie_Ashy_SPB_45.15930.15930.2 2.0138 0.0825 17.9 2101.5522 2099.4832 6427.1 463 3.654 25.0 1 A.YHSLFFTPDKFSKPKKK.K * Jamie_Ashy_SPB_77.27709.27709.3 2.738 0.1437 15.4 3213.4143 3214.7942 9267.9 9 3.441 22.2 1 R.KLSEPSASTRTRYYLKEAKMATKKALQL.D * Jamie_Ashy_SPB_22.07764.07764.3 2.1807 0.0854 73.2 1354.3444 1356.5823 6640.1 83 3.088 40.0 1 K.FVNFTQMALER.S Reverse_YNL243W 2 2 5.6% 968 108996 5.5 U SLA2 SGDID:S000005187, Chr XIV from 188052-190958, Verified ORF, "Transmembrane actin-binding protein involved in membrane cytoskeleton assembly and cell polarization; adaptor protein that links actin to clathrin and endocytosis; present in the actin cortical patch of the emerging bud tip; dimer in vivo" * Jamie_Ashy_SPB_67.24140.24140.3 2.5547 0.1494 15.3 2176.7043 2176.4343 5118.5 104 3.577 27.6 1 R.LHESSKVIKDATAVLESHPN.T * Jamie_Ashy_SPB_30.10688.10688.4 3.4966 0.1765 44.3 4063.7366 4059.6367 6338.4 5 3.894 18.7 1 D.WTYVICARVHKRKPATEEVSCAKKLAKQLDSDIR.S Reverse_YNL095C 2 2 5.6% 642 71196 7.6 U YNL095C SGDID:S000005039, Chr XIV from 446842-444914, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_61.21842.21842.2 1.7996 0.0786 83.6 2191.5723 2190.4155 8744.5 389 3.031 22.5 1 K.TIKSNSSTDPLSLPAISNTSR.R * Jamie_Ashy_SPB_66.23631.23631.2 1.9093 0.1764 39.1 1717.0122 1715.7246 6678.3 60 3.845 35.7 1 K.TTESVRVASEDDNYE.F Reverse_YHR202W 2 2 5.6% 602 68998 6.2 U YHR202W SGDID:S000001245, Chr VIII from 502388-504196, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_42.15128.15128.3 2.5045 0.1302 83.2 2075.0942 2078.4624 7199.6 4 3.463 33.8 1 K.NLYTEVRSAVVLPVTIFR.W * Jamie_Ashy_SPB_28.10113.10113.2 1.9868 0.1054 10.5 1803.6122 1803.9635 8493.4 2 3.494 40.0 1 Q.STGRYELIASEATYLE.H YPR184W 3 3 5.5% 1536 174971 5.8 U GDB1 SGDID:S000006388, Chr XVI from 902040-906650, Verified ORF, "Glycogen debranching enzyme containing glucanotranferase and alpha-1,6-amyloglucosidase activities, required for glycogen degradation" * Jamie_Ashy_SPB_100.35818.35818.3 2.6847 0.1563 90.8 2018.1843 2021.1106 4019.3 110 3.849 30.9 1 K.DNVTEPNFGTLGERNSNR.I * Jamie_Ashy_SPB_15.05323.05323.3 2.6729 0.1036 14.2 2304.0244 2302.5835 6028.3 2 3.089 31.6 1 K.ELEGFDISYDDSKKISTIKL.P * Jamie_Ashy_SPB_91.32558.32558.4 2.4492 0.2269 16.2 5672.177 5677.454 7501.7 106 4.869 11.6 1 F.KTSPRCQNAAKEKPSSYLYQQLYYRLKGHRKWIFESVWAGLTELTNK.D YIL159W 2 2 5.5% 1375 156851 7.9 U BNR1 SGDID:S000001421, Chr IX from 41825-45952, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1" * Jamie_Ashy_SPB_107.38348.38348.4 3.7777 0.1927 63.3 4123.0566 4118.824 4867.5 227 4.539 17.2 1 E.ILNFLKKNNIHNTITFLELAYKDDPNSVVIMEQIK.Q * Jamie_Ashy_SPB_77.27615.27615.4 2.9004 0.1194 17.1 4653.5366 4658.3965 7088.6 37 3.624 15.4 1 L.VENHDKDHLIAKLDESESLISLKTKEIENLKLQLKATKKR.L YNL278W 2 2 5.5% 1060 118280 8.9 U CAF120 SGDID:S000005222, Chr XIV from 113271-116453, Verified ORF, "Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation" * Jamie_Ashy_SPB_16.05554.05554.4 2.6733 0.0453 59.2 4171.1367 4171.668 6119.9 382 2.795 15.7 1 R.QYITEHRSRHEVPKRSPEKPSNPLEIGNPYAKPGTR.L * Jamie_Ashy_SPB_69.24770.24770.2 1.6214 0.1664 19.9 2353.632 2355.6128 11251.0 26 4.154 28.6 1 R.SITPQRGQPVPSGQQISSYVQP.A Reverse_YKL157W 2 2 5.5% 935 105585 7.9 U APE2 SGDID:S000001640, Chr XI from 154996-155276,155660-158186, Verified ORF, "Zinc-dependent metallopeptidase yscII, may have a role in obtaining leucine from dipeptide substrates; sequence coordinates have changed since RT-PCR analysis showed that the adjacent ORF YKL158W comprises the 5' exon of APE2/YKL157W" * Jamie_Ashy_SPB_56.19890.19890.4 2.8919 0.1054 16.7 3731.6565 3729.1465 8731.0 163 3.211 17.2 1 I.AEKNGSCYDTFMKLAAKEVDADRAACAAGFMTVK.L * Jamie_Ashy_SPB_83.29641.29641.2 1.7772 0.1373 16.8 1979.5521 1977.1139 6262.9 7 3.547 40.6 1 K.NLLQSTKQCKACAHSTE.T YGL141W 2 2 5.5% 910 105566 6.4 U HUL5 SGDID:S000003109, Chr VII from 238358-241090, Verified ORF, "Protein with similarity to hect domain E3 ubiquitin-protein ligases, not essential for viability" * Jamie_Ashy_SPB_47.16682.16682.4 2.9505 0.1274 15.0 2971.4565 2970.4084 5329.3 249 3.172 23.2 1 R.TRNSKKDLLEKAKRERERRAQDKL.K * Jamie_Ashy_SPB_31.11120.11120.3 2.91 0.167 11.1 2857.1042 2853.1956 6450.5 289 3.944 22.0 1 D.LTFEIDEPESSAKVVDLIPNGSKTYV.T YMR066W 2 2 5.5% 898 104748 8.6 U SOV1 SGDID:S000004670, Chr XIII from 401540-404236, Uncharacterized ORF, "Mitochondrial protein of unknown function" * Jamie_Ashy_SPB_40.14370.14370.4 3.441 0.1701 39.1 3962.3765 3967.7146 6169.4 35 3.724 16.7 1 R.FNGIIKPKESITEMVTFSQLAQAFEVVKLIPVQEM.K * Jamie_Ashy_SPB_18.06168.06168.4 2.4378 0.083 75.9 1664.1365 1659.9849 3670.6 18 3.082 34.6 1 K.LRKAELMNNESILK.N YDR466W 2 2 5.5% 898 100476 9.5 U PKH3 SGDID:S000002874, Chr IV from 1395111-1397807, Verified ORF, "Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant" * Jamie_Ashy_SPB_26.09140.09140.3 2.737 0.1733 10.1 2426.1843 2423.7341 4999.2 385 3.672 26.2 1 W.RGIWQSQGQSLQQTTLGLPNIP.Q * Jamie_Ashy_SPB_44.15601.15601.3 2.8249 0.1154 18.4 2862.3843 2864.1074 7232.3 63 3.617 21.2 1 R.IPSSTKDRPGANSTPSSRHPRVLSSNN.A YLL013C 2 2 5.5% 879 98068 7.2 U PUF3 SGDID:S000003936, Chr XII from 124713-122074, reverse complement, Verified ORF, "Protein that regulates degradation of specific mRNAs such as COX17, binds almost exclusively to cytoplasmic mRNAs encoding mitochondrial proteins, member of the PUF protein family that contains Pumilio homology domains" * Jamie_Ashy_SPB_54.19333.19333.3 3.128 0.2376 60.8 2770.9744 2769.9102 7690.8 1 4.232 23.3 1 L.SHSNNNGGQAAAAGIVNGGAAGSQQIGGFR.R * Jamie_Ashy_SPB_62.22160.22160.2 1.7194 0.1224 13.2 1940.5122 1942.0121 7775.3 479 3.405 29.4 1 K.KNASSKSNESTANNHKSH.S YHR031C 3 3 5.5% 723 81581 9.7 U RRM3 SGDID:S000001073, Chr VIII from 172963-170792, reverse complement, Verified ORF, "DNA helicase involved in rDNA replication and Ty1 transposition; structurally and functionally related to Pif1p" * Jamie_Ashy_SPB_9.03204.03204.2 1.5328 0.0692 13.3 955.33215 955.1887 4800.4 58 3.088 78.6 1 F.KKLVDVPR.L * Jamie_Ashy_SPB_31.11191.11191.4 2.4504 0.0332 49.8 2390.8965 2391.686 6104.3 54 3.393 24.2 1 K.KLQSLPGDLYEFKAVDNAPER.Y * Jamie_Ashy_SPB_11.03750.03750.3 2.1714 0.2337 28.6 1306.9443 1310.502 2436.2 424 4.051 32.5 1 F.RQDLNARPLAR.L Reverse_YLR072W 2 2 5.5% 693 78212 6.2 U YLR072W SGDID:S000004062, Chr XII from 278863-280944, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern" * Jamie_Ashy_SPB_9.02980.02980.2 1.9908 0.1154 29.3 1235.3922 1234.4435 5506.9 27 3.223 55.6 1 E.YQFPANPKLR.Y * Jamie_Ashy_SPB_115.41217.41217.2 1.2997 0.1379 12.5 2726.5322 2726.8396 5546.7 26 3.885 16.7 1 K.ADGDVGDSDGNTSTAMIINNVSTLATEG.G Reverse_YDR320C 2 2 5.5% 668 75020 5.3 U SWA2 SGDID:S000002728, Chr IV from 1108096-1106090, reverse complement, Verified ORF, "Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles" * Jamie_Ashy_SPB_12.04110.04110.3 2.7517 0.111 17.8 2198.9343 2199.5884 5943.3 2 3.587 35.3 1 V.PMSKKVPPPNIFDQCRRK.G * Jamie_Ashy_SPB_99.35576.35576.3 2.1754 0.0873 60.8 2086.5544 2090.471 3936.9 126 3.25 29.2 1 K.SYEGIKLQSAIINSLAIIR.L Reverse_YMR171C 2 2 5.5% 550 62532 4.8 U YMR171C SGDID:S000004781, Chr XIII from 605519-603867, reverse complement, Uncharacterized ORF, "Endosomal protein of unknown function, mRNA is targeted to the bud via the mRNA transport system involving She2p" * Jamie_Ashy_SPB_13.04465.04465.3 2.0528 0.0379 43.1 1709.3644 1709.992 6755.6 64 2.727 35.7 1 K.WGRIHGGVSKENLKK.G * Jamie_Ashy_SPB_18.06471.06471.3 2.3682 0.1176 12.0 1696.3143 1696.9067 4845.4 160 3.651 39.3 1 R.YGIGVIDGKECQPFL.T YDL020C 2 2 5.5% 531 60153 5.4 U RPN4 SGDID:S000002178, Chr IV from 416705-415110, reverse complement, Verified ORF, "Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses" * Jamie_Ashy_SPB_9.03240.03240.4 2.4213 0.2058 15.6 1713.9365 1716.9816 5277.2 84 3.954 28.9 1 I.KKPTSAVVSSNASRRK.L * Jamie_Ashy_SPB_72.25809.25809.2 1.6941 0.1152 89.3 1388.7722 1386.4209 5262.8 8 3.044 50.0 1 K.HLSSHSSTNSNSK.P Reverse_YBL045C 2 2 5.5% 457 50228 7.3 U COR1 SGDID:S000000141, Chr II from 135519-134146, reverse complement, Verified ORF, "Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain" * Jamie_Ashy_SPB_15.05260.05260.2 2.1403 0.1472 40.6 2244.392 2244.5095 5861.8 14 4.405 30.0 1 A.GLLNADNVPNGSEYLQGLQLK.L * Jamie_Ashy_SPB_3.01116.01116.2 2.4076 0.0428 37.6 831.1722 830.0586 4410.1 39 3.042 91.7 1 L.QLKLLSK.A Reverse_YCR012W 2 2 5.5% 416 44738 7.6 U PGK1 SGDID:S000000605, Chr III from 137743-138993, Verified ORF, "3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis" * Jamie_Ashy_SPB_34.12018.12018.2 1.4895 0.0938 12.3 1005.7522 1005.2052 3244.9 38 3.241 62.5 1 E.KELLFGAAR.Q * Jamie_Ashy_SPB_17.05839.05839.2 1.837 0.075 15.7 1676.5922 1675.7983 6456.9 348 3.093 34.6 1 Q.GDVKRSGEEEIHYR.L Reverse_YHR083W 2 2 5.5% 329 37404 7.2 U SAM35 SGDID:S000001125, Chr VIII from 272629-273618, Verified ORF, "Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane" * Jamie_Ashy_SPB_21.07424.07424.2 1.5078 0.0794 10.5 1203.9521 1203.2975 5229.3 56 3.243 61.1 1 L.QEADDLIRSR.M * Jamie_Ashy_SPB_8.02773.02773.2 1.7101 0.0397 11.8 915.4522 916.1117 5366.5 34 2.527 78.6 1 I.AVTSRIIR.K Reverse_YER132C 4 4 5.4% 1753 195382 7.8 U PMD1 SGDID:S000000934, Chr V from 430445-425184, reverse complement, Verified ORF, "Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions" * Jamie_Ashy_SPB_77.27756.27756.4 2.6867 0.1852 14.0 5102.5767 5105.566 6933.4 450 4.006 13.3 1 R.NATASRDECHGESEKRGTNVASPMFAPNMFLASTSHTTQTSFRKNM.H * Jamie_Ashy_SPB_26.09339.09339.2 2.1441 0.2506 29.4 1487.0122 1487.612 4820.0 124 4.406 39.3 1 Q.VGSNSPFAISGISNH.S * Jamie_Ashy_SPB_34.12150.12150.2 2.4858 0.2455 28.6 1742.8722 1743.7125 7229.1 42 5.082 35.7 1 D.GDEEEDRGNNMGYEQ.I * Jamie_Ashy_SPB_107.38407.38407.2 1.7155 0.124 15.3 2202.0923 2204.5 5206.1 238 3.369 25.0 1 S.TQRSQNPSAIFRLASSKRR.S YMR247C 4 4 5.4% 1562 180185 5.5 U YMR247C SGDID:S000004861, Chr XIII from 768038-763350, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_56.20127.20127.4 2.815 0.1357 16.5 4833.0166 4838.687 8111.2 6 3.424 15.4 1 E.SIWKLLNLKNGQNTNAYETVLRLIDVLYTRGYMPSHKNIMK.L * Jamie_Ashy_SPB_46.16530.16530.2 1.7873 0.0862 15.4 1560.2122 1558.605 6367.4 6 2.927 45.8 1 S.CALYEVSEDTNEK.L * Jamie_Ashy_SPB_12.04162.04162.2 1.928 0.1128 94.0 1727.0122 1727.975 5679.5 90 3.949 37.5 1 K.NKFHGACLYKWFR.S * Jamie_Ashy_SPB_19.06625.06625.2 1.6416 0.0458 70.3 2155.112 2152.307 5629.2 299 3.448 26.5 1 R.SSGNNTCPLCRSEIPFRR.- Reverse_YDR464W 3 3 5.4% 1435 161596 8.9 U SPP41 SGDID:S000002872, Chr IV from 1388862-1393169, Verified ORF, "Protein involved in negative regulation of expression of spliceosome components PRP4 and PRP3" * Jamie_Ashy_SPB_89.32056.32056.3 1.3942 0.0096 33.2 4877.724 4873.9272 11541.6 84 2.515 11.9 1 R.KRYKKINPIKKRRAVPLAFPPQSPITWPPHFPIKEKHVVTK.L * Jamie_Ashy_SPB_56.20197.20197.2 1.6322 0.0737 78.9 1518.7522 1517.6823 6337.1 6 2.928 54.5 1 R.EERATQRELKEK.E * Jamie_Ashy_SPB_42.14878.14878.3 2.3797 0.0914 66.9 2619.8342 2623.0256 5835.3 63 3.298 22.9 1 K.KKKKKKSTSESAPSKNATSAAKSTK.S YPL016W 2 2 5.4% 1314 147937 8.8 U SWI1 SGDID:S000005937, Chr XVI from 521011-524955, Verified ORF, "One of 11 subunits of the SWI/SNF chromatin remodeling complex that regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2" * Jamie_Ashy_SPB_20.07050.07050.4 2.2203 0.2377 10.8 6138.1367 6137.6235 6746.6 395 3.817 10.1 1 S.TNFTSPTAVVNNAAPANVTGGKAANFIQNQSPQFNSPYDSNNSNTNLNSLSPQAILAKN.S * Jamie_Ashy_SPB_13.04538.04538.2 2.2531 0.0282 16.4 1424.6921 1423.5283 6735.2 109 2.675 45.5 1 I.LRNISFSDNNSR.I YDL145C 2 2 5.4% 1201 135607 6.0 U COP1 SGDID:S000002304, Chr IV from 198177-194572, reverse complement, Verified ORF, "Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway" * Jamie_Ashy_SPB_45.15964.15964.3 2.486 0.0113 42.7 3911.8442 3909.6226 10411.3 20 3.803 16.9 1 K.VSQLAVKNVKYVSWSLDGQYVALMSKHTITLATKK.L * Jamie_Ashy_SPB_83.29713.29713.2 1.2593 0.1432 10.4 3220.612 3220.7986 8963.9 5 3.318 19.0 1 L.TGSKYVITEKDKIDRIAMISKIGAPASGLR.I YJL076W 3 3 5.4% 1189 128531 7.8 U NET1 SGDID:S000003612, Chr X from 295161-298730, Verified ORF, "Core subunit of the RENT complex, which is a complex involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure" * Jamie_Ashy_SPB_97.34798.34798.2 1.4646 0.1243 12.6 1773.3121 1776.0886 7060.1 364 3.322 30.0 1 R.SFLPPPTQPQSPPIRI.S * Jamie_Ashy_SPB_67.24000.24000.3 2.1122 0.0108 35.8 3173.7544 3173.634 7662.2 249 3.042 17.9 1 K.LLEKEILPTIPHNDQPILASSDKSNGTLK.S * Jamie_Ashy_SPB_87.31174.31174.2 1.9974 0.1041 17.3 2049.2722 2050.4954 7105.7 286 3.665 27.8 1 A.LGKKKKPSGGFASLIKDFK.K Reverse_YIL112W 3 3 5.4% 1083 123556 5.7 U HOS4 SGDID:S000001374, Chr IX from 151592-154843, Uncharacterized ORF, "Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate" * Jamie_Ashy_SPB_90.32203.32203.2 1.9008 0.0857 15.4 2328.4722 2329.6794 7625.0 11 3.93 31.6 1 R.AFENISIQIMNGEIEKRKAH.N * Jamie_Ashy_SPB_41.14682.14682.2 1.562 0.0757 10.4 1310.7122 1311.524 5631.1 75 3.492 45.5 1 K.SIEVSKAKKPSH.P * Jamie_Ashy_SPB_34.12158.12158.4 3.3179 0.0703 16.3 2930.9766 2932.225 4908.1 134 3.311 21.3 1 R.STQNVPFGFALFISTIDEHGCKVSEF.F YDR379W 2 2 5.4% 1009 113291 7.9 U RGA2 SGDID:S000002787, Chr IV from 1230157-1233186, Verified ORF, "GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth" * Jamie_Ashy_SPB_57.20346.20346.4 2.7964 0.0129 51.3 4219.8564 4221.6016 7522.0 299 2.648 15.3 1 K.HPHHSTSIQESDTHSGWGVSSTHTNIRKSKAKKNPVSR.G * Jamie_Ashy_SPB_47.16884.16884.2 1.8097 0.0569 12.2 1605.3522 1605.705 7899.5 47 3.285 36.7 1 Q.RSPNSSSGGTTNIAQK.E YBR133C 2 2 5.4% 827 95153 6.4 U HSL7 SGDID:S000000337, Chr II from 504281-501798, reverse complement, Verified ORF, "Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p" * Jamie_Ashy_SPB_62.22147.22147.2 1.8325 0.038 11.0 2480.1921 2480.9531 7883.9 37 3.413 25.0 1 Y.DYVLLPITTPRYKEIVGQVFK.D * Jamie_Ashy_SPB_25.08722.08722.2 3.162 0.4184 100.0 2810.652 2811.0374 5758.4 1 6.233 39.1 1 K.ALEPSNELPRHEDLEEDVPEVHVR.V YJR106W 2 2 5.4% 725 80657 6.8 U ECM27 SGDID:S000003867, Chr X from 624751-626928, Verified ORF, "Non-essential protein of unknown function" * Jamie_Ashy_SPB_18.06282.06282.2 2.0868 0.0251 13.8 1400.3322 1401.5798 5091.3 427 2.968 50.0 1 L.FSNLMSWATSTR.E * Jamie_Ashy_SPB_52.18433.18433.4 3.141 0.0862 17.2 3030.6165 3032.72 6850.3 245 3.169 22.4 1 R.LTKEMKLVGISMCGLWALATLINILLE.L YJL149W 2 2 5.4% 663 77367 6.7 U YJL149W SGDID:S000003685, Chr X from 137297-139288, Uncharacterized ORF, "Hypothetical protein; has similarity to F-box proteins" * Jamie_Ashy_SPB_26.09134.09134.4 3.5704 0.2006 64.3 3513.5764 3516.0068 5802.6 312 4.053 20.2 1 Q.WIRINWDLDSTLQRSILSILCNQGKSLQR.L * Jamie_Ashy_SPB_23.08167.08167.2 1.7354 0.1518 34.0 885.39215 885.05676 2758.2 37 3.792 58.3 1 L.KSFPHLR.F Reverse_YOL158C 2 2 5.4% 606 66970 9.4 U ENB1 SGDID:S000005518, Chr XV from 21310-19490, reverse complement, Verified ORF, "Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment" * Jamie_Ashy_SPB_20.06950.06950.2 2.251 0.345 96.3 1993.3722 1995.3881 3319.3 21 5.234 35.3 1 P.SKYIAMGLSSGLRQILEK.A * Jamie_Ashy_SPB_97.34772.34772.2 1.6731 0.0754 12.8 1551.3522 1548.8229 4439.9 175 3.068 35.7 1 R.LGSLDGTLATALLRF.G Reverse_YBL005W-B 5 5 5.3% 1755 198968 8.2 U YBL005W-B SGDID:S000002147, Chr II from 221333-222637,222639-226601, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Reverse_YBR012W-B 5 5 5.3% 1756 198868 8.5 U YBR012W-B SGDID:S000002155, Chr II from 259867-261171,261173-265138, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Ashy_SPB_52.18705.18705.2 1.5897 0.1276 15.9 1340.3722 1339.6646 5628.4 180 3.734 40.9 1 V.VLKNTPKVPKSK.H Jamie_Ashy_SPB_2.00541.00541.2 2.1635 0.0472 17.7 1243.2922 1242.4606 4468.7 127 2.94 61.1 1 H.PPPRIYLEEK.I Jamie_Ashy_SPB_103.36914.36914.4 2.7041 0.0197 50.8 4362.4165 4358.0405 7782.9 86 2.985 17.1 1 K.HYAEIYKEKEKIDKNYTIAEDYRLTTRIPKISKVAK.V Jamie_Ashy_SPB_55.19845.19845.2 1.9789 0.1236 23.7 1742.2522 1741.9419 8750.0 3 4.215 40.0 1 G.GLSSNTQRSNIPPLEK.F Jamie_Ashy_SPB_85.30410.30410.2 1.7425 0.0648 12.8 2085.8123 2083.324 8326.3 401 3.003 27.8 1 Y.SSEHTNSQSMPALLPVDLR.H Reverse_YBL079W 4 4 5.3% 1502 169474 6.1 U NUP170 SGDID:S000000175, Chr II from 75256-79764, Verified ORF, "Abundant subunit of the nuclear pore complex (NPC), required for proper localization of specific nucleoporins within the NPC, involved in nuclear envelope permeability and in chromosome segregation, has similarity to Nup157p" * Jamie_Ashy_SPB_62.22125.22125.2 1.6371 0.0635 10.1 2907.7122 2908.3618 8359.3 288 2.989 22.0 1 S.LPVAFDNFLESTSLIKGDLTKILEDK.I * Jamie_Ashy_SPB_22.07951.07951.2 1.89 0.023 13.3 1268.5322 1267.4216 5981.8 34 2.969 55.0 1 F.DNFLESTSLIK.G * Jamie_Ashy_SPB_52.18615.18615.4 3.2537 0.0717 15.0 4194.9365 4190.74 9995.6 189 3.165 14.9 1 P.SPKSFGTISQQLNTTATSLTPKSLLSSVTSVSYRNYVPK.I * Jamie_Ashy_SPB_58.20793.20793.2 1.9729 0.1096 20.2 1567.8522 1565.8503 6052.2 49 4.124 38.5 1 K.YIEISTSTLVAVRL.T YJL095W 3 3 5.3% 1478 164195 9.5 U BCK1 SGDID:S000003631, Chr X from 247171-251607, Verified ORF, "Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p" * Jamie_Ashy_SPB_26.09151.09151.4 2.7893 0.1571 12.3 4101.8164 4096.4624 6251.8 55 3.328 18.0 1 R.PHSIIESNSTLTKSASPPASPSYPSIFRRHHKSSSSES.S * Jamie_Ashy_SPB_109.39103.39103.2 1.834 0.0262 10.1 1497.8322 1496.753 4118.6 21 3.33 41.7 1 K.NKPLNQAKTPKRT.K * Jamie_Ashy_SPB_15.05370.05370.2 1.2802 0.1589 12.8 2968.412 2969.4717 8154.9 42 3.526 19.2 1 K.RPWSNLEVVAAMFKIGKSKSAPPIPED.T Reverse_YLR398C 2 2 5.3% 1287 146058 6.7 U SKI2 SGDID:S000004390, Chr XII from 919019-915156, reverse complement, Verified ORF, "Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; required for repressing propagation of dsRNA viruses" * Jamie_Ashy_SPB_29.10292.10292.4 2.7339 0.1261 83.6 4596.2163 4598.496 5792.2 72 3.426 16.2 1 K.KIVKGLPAAFKRKTIVEIATVPVEEMYFDTKQFKPFNRR.R * Jamie_Ashy_SPB_13.04484.04484.4 3.2219 0.1848 95.5 2729.1765 2729.855 5440.0 77 4.06 21.4 1 K.NSGIAGAGRSGGRNAGGRTSNGRGGRGDR.T YDR097C 2 2 5.3% 1242 140080 5.8 U MSH6 SGDID:S000002504, Chr IV from 643832-640104, reverse complement, Verified ORF, "Protein required for mismatch repair in mitosis and meiosis, forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; potentially phosphorylated by Cdc28p" * Jamie_Ashy_SPB_114.41062.41062.4 2.7102 0.1109 12.3 4047.0566 4050.7004 7199.0 5 4.376 17.6 1 K.IAGGGRANMQLAGIPEMSFEYWAAQFIQMGYKVAKVD.Q * Jamie_Ashy_SPB_75.26778.26778.4 2.8253 0.1122 17.8 3319.6965 3320.84 5046.4 4 3.548 23.2 1 T.HYGTLASSFKHHPQVRPLKMSILVDEATR.N Reverse_YOR207C 3 3 5.3% 1149 129456 8.2 U RET1 SGDID:S000005733, Chr XV from 733457-730008, reverse complement, Verified ORF, "Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs" * Jamie_Ashy_SPB_29.10286.10286.2 1.7764 0.0688 12.4 2154.2322 2155.3938 9299.3 77 3.374 33.3 1 R.ELLLQSAGYAIVCDREMEG.L * Jamie_Ashy_SPB_85.30340.30340.2 2.0113 0.0754 15.9 1916.8522 1918.9653 5866.8 208 3.398 31.2 1 K.SSEELNVAFIDQYSSDN.G * Jamie_Ashy_SPB_46.16598.16598.2 1.7786 0.1485 22.1 2785.152 2786.0486 3894.8 235 4.078 22.9 1 K.ETGNVIFYGGPDLPCENLKAMKSED.A YBL101C 2 2 5.3% 1117 123609 6.9 U ECM21 SGDID:S000000197, Chr II from 28299-24946, reverse complement, Verified ORF, "Non-essential protein of unknown function; promoter contains several Gcn4p binding elements" * Jamie_Ashy_SPB_29.10419.10419.2 1.7285 0.0669 12.1 1038.4521 1040.1643 4252.7 25 3.638 61.1 1 K.SNSGLPVKTH.T * Jamie_Ashy_SPB_49.17642.17642.4 2.713 0.1262 19.1 5256.0166 5258.9336 8298.5 11 3.975 14.2 1 F.FGNTCPPPPTFEEAISVPASPIVSPMGSPNIMASYDPDLLSIQQLNLSR.T YGL241W 3 3 5.3% 1004 113921 4.7 U KAP114 SGDID:S000003210, Chr VII from 45445-48459, Verified ORF, "Karyopherin, responsible for nuclear import of Spt15p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear" * Jamie_Ashy_SPB_43.15315.15315.2 1.7222 0.0601 77.2 2054.7122 2052.4192 8230.3 191 2.882 26.5 1 K.TLENILVSQEIPELILAR.A * Jamie_Ashy_SPB_61.21990.21990.2 1.9367 0.1404 28.5 1883.3922 1885.2981 6083.9 339 3.219 28.1 1 F.LAKSLNLALKSDKELIK.S * Jamie_Ashy_SPB_63.22512.22512.3 2.7773 0.0947 15.6 2155.6743 2154.6409 5772.2 11 3.963 32.4 1 R.YVQVPLYTKIIKLFVSEL.S Reverse_YGR056W 2 2 5.3% 928 106669 6.5 U RSC1 SGDID:S000003288, Chr VII from 601665-604451, Verified ORF, "One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook" * Jamie_Ashy_SPB_70.24898.24898.4 3.8003 0.2011 69.8 3917.2566 3921.5254 6014.1 27 4.24 16.7 1 G.VLPPATPAGETPEPVKDNLTANAPLLDAIPSPYKFFK.R * Jamie_Ashy_SPB_12.04329.04329.2 1.5988 0.0212 65.7 1513.0322 1513.6934 4951.4 15 3.108 50.0 1 R.IKNFVKDSENYR.F Reverse_YGL163C 2 2 5.3% 898 101754 9.0 U RAD54 SGDID:S000003131, Chr VII from 196409-193713, reverse complement, Verified ORF, "DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family" * Jamie_Ashy_SPB_63.22396.22396.4 3.0857 0.1245 12.1 2671.0166 2665.0178 4210.8 160 3.456 25.8 1 S.PDYLVIAFEDTPDHLPRPEPTIK.K * Jamie_Ashy_SPB_110.39628.39628.4 2.2687 0.0031 36.6 2938.0166 2939.3938 3687.2 397 2.809 22.2 1 R.LTYQIHSLRTPDKALRQASLADKRR.K YDR213W 2 2 5.3% 913 100339 6.0 U UPC2 SGDID:S000002621, Chr IV from 889746-892487, Verified ORF, "Sterol regulatory element binding protein, induces transcription of sterol transport and biosynthetic genes; involved in the anaerobic induction of DAN/TIR mannoproteins and seripauperins; binucleate zinc cluster protein; Ecm22p homolog" * Jamie_Ashy_SPB_54.19245.19245.2 1.9758 0.0635 16.8 2122.0923 2120.463 6332.7 161 3.438 30.6 1 D.GKKVSTTSTGKRKFHNKSK.N * Jamie_Ashy_SPB_29.10345.10345.4 3.1231 0.1505 12.0 3076.3765 3077.3906 6783.9 254 3.522 19.0 1 P.STPSAVLNDRQEMQDSISSLGNLTKAALE.N Reverse_YIL038C 2 2 5.3% 836 94403 5.6 U NOT3 SGDID:S000001300, Chr IX from 282651-280141, reverse complement, Verified ORF, "Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation" * Jamie_Ashy_SPB_21.07288.07288.2 1.8706 0.0941 18.0 998.6722 997.1826 4854.4 3 3.371 68.8 1 N.VGQPVEVLR.S * Jamie_Ashy_SPB_76.27202.27202.3 1.8279 0.0066 33.3 4326.744 4325.881 9682.1 278 3.235 14.7 1 K.NLLLLKDIEVQLSDYQRELEDIMQSLYESIDRRER.E Reverse_YCL045C 2 2 5.3% 760 87181 5.3 U YCL045C SGDID:S000000550, Chr III from 46905-44623, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_31.11175.11175.2 2.5146 0.0756 31.9 1347.2322 1345.5969 5500.2 8 3.655 63.6 1 T.IQGNELEMVIAK.T * Jamie_Ashy_SPB_12.04094.04094.4 3.7411 0.0308 13.5 3183.3765 3177.4358 3427.5 40 3.248 22.2 1 N.SSSNVCVMANHDNSDLQIEDITFPLVNR.F YGL110C 2 2 5.3% 624 72043 7.0 U CUE3 SGDID:S000003078, Chr VII from 303414-301540, reverse complement, Uncharacterized ORF, "Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination" * Jamie_Ashy_SPB_9.03018.03018.3 2.524 0.0395 66.4 1698.2344 1701.0403 4064.3 229 3.073 38.5 1 R.MLERSPTRARLLEK.K * Jamie_Ashy_SPB_57.20247.20247.4 1.9779 0.0367 38.7 2291.4565 2296.5889 4457.6 59 2.677 29.6 1 K.QKKSENIKKHEPQSTEQKK.R Reverse_YLL011W 2 2 5.3% 489 56789 9.4 U SOF1 SGDID:S000003934, Chr XII from 127522-128991, Verified ORF, "Essential subunit of the U3 (box C+D) snRNP complex required for 2' O-methylation of pre-RNA; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p" * Jamie_Ashy_SPB_21.07501.07501.2 1.6433 0.0491 74.5 1502.0521 1502.6766 6346.9 2 3.516 60.0 1 R.KDDEKRRKRDR.G * Jamie_Ashy_SPB_3.00783.00783.3 2.1836 0.1585 83.9 1641.1444 1641.8241 5458.1 240 3.797 35.7 1 K.NLSGYNKAIAYVGDR.H YJL222W 2 2 5.2% 1549 174401 5.3 U VTH2 SGDID:S000003758, Chr X from 11475-16124, Verified ORF, "Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in vacuolar protein sorting" Jamie_Ashy_SPB_107.38414.38414.4 2.8621 0.1928 24.4 4264.4165 4260.8643 5040.6 7 3.636 17.1 1 I.SNDVSTFQVARTPTKIRHVNMGQIHEDSIGRIVLPVSR.E * Jamie_Ashy_SPB_54.19392.19392.4 3.5138 0.0695 17.6 4452.4966 4450.6934 11676.2 399 3.171 14.6 1 V.DTSQQEHTSRTDLLGASNIPDALPARSASHESDLAAARSEDK.- YML104C 2 2 5.2% 1127 129981 6.4 U MDM1 SGDID:S000004572, Chr XIII from 62322-58939, reverse complement, Verified ORF, "Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds" * Jamie_Ashy_SPB_25.08990.08990.4 2.7138 0.1122 17.8 2014.9365 2016.5334 4761.4 362 3.477 31.4 1 K.LMKNDSASLIVLKLLPIF.N * Jamie_Ashy_SPB_48.16975.16975.4 3.8394 0.075 89.9 4464.4565 4463.877 7453.5 14 2.975 14.6 1 R.NMEKVSVSSGNSGLNPSQFYGSNNFRDNIASLTISIDQIEK.E Reverse_YHR154W 4 4 5.2% 1070 123017 7.8 U RTT107 SGDID:S000001197, Chr VIII from 402969-406181, Verified ORF, "Protein that interacts with Mms22p and is implicated in Mms22-dependent DNA repair during S phase, damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; has four BRCT domains; has a role in regulation of Ty1 transposition" * Jamie_Ashy_SPB_117.41911.41911.2 1.796 0.0501 12.4 1535.8722 1533.8175 5244.3 2 3.36 45.8 1 R.LIKPAFICNLKDT.E * Jamie_Ashy_SPB_12.04296.04296.3 2.2059 0.0467 53.6 1616.6344 1618.8771 6697.5 37 3.138 33.3 1 K.ENKKSTKIERKTR.Q * Jamie_Ashy_SPB_13.04666.04666.2 2.3576 0.1201 39.5 1857.5922 1857.027 4274.7 466 3.631 32.1 1 P.ADYETIPKTFEKEER.K * Jamie_Ashy_SPB_17.06056.06056.2 1.8233 0.0243 72.0 1542.8522 1542.686 4684.7 148 2.921 42.9 1 R.ELDGSHTVAVEGSIK.Q YDR430C 2 2 5.2% 989 112180 6.4 U CYM1 SGDID:S000002838, Chr IV from 1328460-1325491, reverse complement, Verified ORF, "Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology" * Jamie_Ashy_SPB_96.34291.34291.3 2.2192 0.0179 38.4 2896.7043 2894.3464 7893.9 172 2.857 20.7 1 R.FRGDLETKGDTLFQDLIRKYIVHK.P * Jamie_Ashy_SPB_53.18813.18813.3 3.0518 0.0826 14.3 3232.2544 3230.689 7631.1 103 3.598 19.2 1 R.EKITALDEQDKKNIFKRGILLQEKQNE.K YDR369C 2 2 5.2% 854 96364 8.2 U XRS2 SGDID:S000002777, Chr IV from 1217570-1215006, reverse complement, Verified ORF, "Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling" * Jamie_Ashy_SPB_64.22951.22951.3 2.6963 0.1946 11.1 3046.7043 3046.41 6623.8 43 4.253 20.4 1 N.IAEADEKHAPFLQNALKPTEDIGKKSGH.S * Jamie_Ashy_SPB_49.17450.17450.2 1.736 0.0549 75.3 1915.7522 1915.2401 7258.3 112 3.374 33.3 1 R.NYVPLKKYSKKDTTTK.W YFL013C 2 2 5.2% 692 78753 5.2 U IES1 SGDID:S000001881, Chr VI from 109924-107846, reverse complement, Verified ORF, "Subunit of the INO80 chromatin remodeling complex" * Jamie_Ashy_SPB_45.16122.16122.2 1.2217 0.0312 61.0 2557.5723 2557.959 6736.6 14 3.071 25.0 1 K.TQEEFLADLKKSQVPNRLKRR.D * Jamie_Ashy_SPB_49.17476.17476.2 1.4915 0.037 63.7 1686.6522 1684.0354 10120.3 363 2.962 32.1 1 K.NLGASKKVLLNKIER.A Reverse_YHR020W 2 2 5.2% 688 77386 6.4 U YHR020W SGDID:S000001062, Chr VIII from 143989-146055, Uncharacterized ORF, "Protein required for cell viability" * Jamie_Ashy_SPB_89.31914.31914.2 2.0469 0.0268 12.4 1773.4122 1774.113 7668.9 34 3.3 40.0 1 Y.WGPFDLAKDVTIGILK.A * Jamie_Ashy_SPB_13.04430.04430.2 1.7287 0.053 10.2 2214.152 2214.4492 4838.8 20 3.515 34.2 1 K.KMYTSDNLQLITDGDVTGLE.H YPR155C 3 3 5.2% 616 70862 6.9 U NCA2 SGDID:S000006359, Chr XVI from 837409-835559, reverse complement, Verified ORF, "Protein involved in regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p" * Jamie_Ashy_SPB_11.03762.03762.2 1.9714 0.1391 50.9 969.4522 971.05804 5026.3 8 4.119 71.4 1 S.HSLEESLR.E * Jamie_Ashy_SPB_5.01694.01694.2 1.8489 0.054 14.9 1000.27216 1000.1429 4883.1 8 2.725 71.4 1 Q.QQLIQDVR.E * Jamie_Ashy_SPB_20.07130.07130.2 1.7504 0.117 13.5 1752.2722 1755.0762 8610.6 74 3.503 36.7 1 T.QLHHPIKNIATGGLVR.S contaminant_KERATIN18 2 2 5.2% 562 59822 8.0 U no description * Jamie_Ashy_SPB_2.00619.00619.2 1.9984 0.1641 99.2 937.2922 939.01886 4564.3 22 4.802 68.8 1 R.RGFSASSAR.L * Jamie_Ashy_SPB_46.16424.16424.2 1.8925 0.0635 12.4 1882.6921 1884.0251 7126.0 268 3.849 26.3 1 K.RISIGGGSCAISGGYGSRAG.A YOL123W 2 2 5.2% 534 59650 5.6 U HRP1 SGDID:S000005483, Chr XV from 87843-89447, Verified ORF, "Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences" * Jamie_Ashy_SPB_30.10579.10579.2 1.9076 0.214 60.9 2005.2722 2008.1176 6011.1 31 4.696 35.3 1 R.GFGFVTYDSADAVDRVCQ.N * Jamie_Ashy_SPB_17.06150.06150.2 1.751 0.0333 10.4 877.53217 877.8027 4216.5 40 2.625 66.7 1 K.SSNNGGNNGG.N YJL159W 2 2 5.2% 387 38619 5.4 U HSP150 SGDID:S000003695, Chr X from 120444-121607, Verified ORF, "O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation" YKL164C 2 2 5.9% 341 34622 6.5 U PIR1 SGDID:S000001647, Chr XI from 142824-141799, reverse complement, Verified ORF, "O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle" Jamie_Ashy_SPB_6.01921.01921.2 2.0583 0.0351 88.4 973.9522 974.10504 4731.1 9 3.861 61.1 1 K.GGILTDGKGR.I Jamie_Ashy_SPB_118.42270.42270.2 1.6034 0.1345 15.9 2063.2722 2060.364 9587.6 458 3.857 26.3 1 K.GGILTDGKGRIGSIVANRQF.Q Reverse_YLL015W 4 4 5.1% 1559 176873 7.7 U BPT1 SGDID:S000003938, Chr XII from 116431-121110, Verified ORF, "ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p" * Jamie_Ashy_SPB_6.02046.02046.2 2.3724 0.0273 21.7 988.27216 989.0733 3583.9 15 3.168 68.8 1 S.LNSGNENIK.V * Jamie_Ashy_SPB_43.15312.15312.2 1.7903 0.0434 10.2 1807.3522 1805.1741 7717.1 41 3.788 34.4 1 E.LIRFLALSLTSKGAGTR.G * Jamie_Ashy_SPB_53.18852.18852.4 3.1036 0.0767 80.5 3377.8564 3379.2231 6859.2 38 3.46 20.2 1 R.TLIMFLFFLVVGLVGCAKIYALYIKTKVR.G * Jamie_Ashy_SPB_36.12742.12742.2 1.4057 0.1965 19.6 2892.2522 2890.0032 9156.6 59 4.304 20.8 1 S.ESRHETQVDSENGNDIPSDFEELLK.K Reverse_YNL192W 3 3 5.1% 1131 129919 6.2 U CHS1 SGDID:S000005136, Chr XIV from 276503-279898, Verified ORF, "Chitin synthase I, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor" * Jamie_Ashy_SPB_19.06624.06624.2 1.6591 0.0906 81.9 2167.652 2167.5588 4986.0 323 3.355 26.5 1 K.YEFNQSAVLPNLLKVFRK.G * Jamie_Ashy_SPB_71.25556.25556.2 1.8819 0.0683 16.1 1399.2322 1399.6453 4706.2 38 3.542 46.2 1 K.GPMTGADLLTVINP.R * Jamie_Ashy_SPB_93.33189.33189.4 3.1764 0.1983 44.6 2989.7366 2992.3704 5213.9 453 3.78 23.3 1 R.MFKFENPLTNANETTTAYQDLLTKSI.P YLR389C 2 2 5.1% 1027 117579 6.7 U STE23 SGDID:S000004381, Chr XII from 902660-899577, reverse complement, Verified ORF, "Metalloprotease involved, with homolog Axl1p, in N-terminal processing of pro-a-factor to the mature form; member of the insulin-degrading enzyme family" * Jamie_Ashy_SPB_85.30438.30438.3 2.9486 0.1661 10.8 3008.1843 3012.479 6073.6 11 4.327 23.1 1 L.NPALTLPRPNEFVSTNFKVDKIDGIKP.L * Jamie_Ashy_SPB_95.34116.34116.2 1.1912 0.0786 63.4 2797.3523 2796.06 8310.6 4 3.271 25.0 1 K.ELNENELDTAKYPTGQLIEDVGAFK.S YNL243W 2 2 5.1% 968 108996 5.5 U SLA2 SGDID:S000005187, Chr XIV from 188052-190958, Verified ORF, "Transmembrane actin-binding protein involved in membrane cytoskeleton assembly and cell polarization; adaptor protein that links actin to clathrin and endocytosis; present in the actin cortical patch of the emerging bud tip; dimer in vivo" * Jamie_Ashy_SPB_54.19344.19344.4 2.5708 0.1404 17.7 2750.8965 2756.0505 7559.0 325 3.301 25.4 1 Q.ERVQQLQQQQAQQELFQQQLQK.A * Jamie_Ashy_SPB_49.17353.17353.4 3.1499 0.1173 14.3 3341.6965 3345.959 8135.6 3 3.87 23.1 1 R.KYESLAKLYSQLRQEHLNLLPRFKKLQ.L YDR351W 2 2 5.1% 864 98971 8.3 U SBE2 SGDID:S000002759, Chr IV from 1178657-1181251, Verified ORF, "Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth" * Jamie_Ashy_SPB_95.34051.34051.3 2.3171 0.1925 13.8 3244.9744 3242.5818 7490.5 32 4.094 20.2 1 K.EEDSTHLKQNQPKKFSTKELMLSEYTE.R * Jamie_Ashy_SPB_117.42100.42100.2 1.7212 0.0813 13.2 1982.8922 1982.2931 8225.8 238 3.081 28.1 1 K.EKYDSRILNPRGIVAHL.L YGR264C 2 2 5.1% 751 85678 6.6 U MES1 SGDID:S000003496, Chr VII from 1021859-1019604, reverse complement, Verified ORF, "Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs" * Jamie_Ashy_SPB_57.20287.20287.4 2.9946 0.0859 12.9 3518.2966 3520.1582 6257.9 340 3.285 19.4 1 S.ELLANLGNFVNRLIKFVNAKYNGVVPKFDPK.K * Jamie_Ashy_SPB_2.00569.00569.2 2.3608 0.017 27.8 908.2322 907.01404 6563.7 22 2.474 100.0 1 N.QFLQENK.L Reverse_YOR019W 2 2 5.1% 730 83366 9.7 U YOR019W SGDID:S000005545, Chr XV from 368126-370318, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_55.19752.19752.3 2.7255 0.1841 11.4 2822.9343 2827.2095 7241.8 11 3.918 21.3 1 S.KFVKFIGVGGGTFTNVTSFSSSSKKSGA.S * Jamie_Ashy_SPB_21.07347.07347.2 2.0211 0.0274 86.9 987.53217 986.2401 3533.3 105 2.97 62.5 1 K.GVIIELTIK.V Reverse_YHR031C 2 2 5.1% 723 81581 9.7 U RRM3 SGDID:S000001073, Chr VIII from 172963-170792, reverse complement, Verified ORF, "DNA helicase involved in rDNA replication and Ty1 transposition; structurally and functionally related to Pif1p" * Jamie_Ashy_SPB_21.07311.07311.2 1.8286 0.066 12.7 1517.7122 1516.7 5149.2 394 3.446 45.8 1 L.QIGGFPDDNKRIR.R * Jamie_Ashy_SPB_85.30633.30633.3 2.5397 0.1503 17.2 2614.1643 2612.0042 5802.9 30 4.015 23.9 1 L.QRIITQLIVSKGTGASGTYFVNTR.K YPL043W 2 2 5.1% 685 77825 9.1 U NOP4 SGDID:S000005964, Chr XVI from 469936-471993, Verified ORF, "Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)" * Jamie_Ashy_SPB_77.27718.27718.2 1.6721 0.0625 75.4 2506.0923 2504.994 9276.9 15 3.739 27.3 1 R.AMNDKALKALARKAVVEFATEVK.N * Jamie_Ashy_SPB_58.20608.20608.2 1.7963 0.1165 19.1 1404.5922 1401.6462 4288.0 129 3.905 45.5 1 H.PLSKEEIIRSTK.E YLR153C 2 2 5.1% 683 75492 6.7 U ACS2 SGDID:S000004143, Chr XII from 447576-445525, reverse complement, Verified ORF, "Acetyl-coA synthetase isoform, required for growth on glucose; expressed under anaerobic conditions" * Jamie_Ashy_SPB_18.06499.06499.3 3.2586 0.2064 98.7 2462.3943 2461.6038 6462.3 41 4.858 26.2 1 R.HAFANPDKPALIYEADDESDNK.I * Jamie_Ashy_SPB_41.14727.14727.2 1.5042 0.2118 28.8 1357.7122 1358.5339 5917.6 9 3.871 50.0 1 L.FLLYTSGSTGSPK.G Reverse_YAL067C 2 2 5.1% 593 68842 8.0 U SEO1 SGDID:S000000062, Chr I from 9017-7236, reverse complement, Verified ORF, "Putative permease, member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide" * Jamie_Ashy_SPB_3.00946.00946.3 2.2855 0.1377 11.8 2303.2144 2304.6934 5061.4 322 3.717 26.5 1 K.WDSFITKWLKIDFVKTEF.D * Jamie_Ashy_SPB_72.25788.25788.3 2.9652 0.1381 13.0 2943.0842 2940.4153 8935.3 5 3.416 25.0 1 T.KWLKIDFVKTEFDSKGTQNEKLRK.R Reverse_YCR089W 3 3 5.0% 1609 166036 4.9 U FIG2 SGDID:S000000685, Chr III from 267430-272259, Verified ORF, "Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating" * Jamie_Ashy_SPB_44.15825.15825.4 3.2478 0.1343 13.3 3328.0166 3332.346 7097.6 34 3.608 19.6 1 K.YTESVKCSYGDCSSSTTKQCESGTCTMTD.T * Jamie_Ashy_SPB_74.26450.26450.2 1.7452 0.1393 10.5 2176.0122 2175.5024 7022.8 260 4.073 25.0 1 A.MSSSRLTPPLSSTADVLTPIT.K * Jamie_Ashy_SPB_70.25220.25220.2 1.3699 0.2729 41.9 3177.1921 3174.4082 9326.7 419 5.011 13.3 1 S.SSPATVPLEFSSSSHSTRHPQATSSLTSSAK.E YOR335C 2 2 5.0% 958 107277 5.5 U ALA1 SGDID:S000005862, Chr XV from 949104-946228, reverse complement, Verified ORF, "Cytoplasmic alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog" * Jamie_Ashy_SPB_41.14752.14752.4 2.9771 0.0672 76.5 3101.7766 3105.4436 5408.5 220 3.206 19.0 1 K.DKSIYLLAGNDPEGRVAHGCYISNAALAK.G * Jamie_Ashy_SPB_10.03510.03510.3 2.4485 0.1938 13.8 1804.5844 1805.0873 5021.5 133 4.141 29.2 1 K.KVSSIIGGKAGGKGNVFQG.M Reverse_YBR294W 2 2 5.0% 859 95951 6.8 U SUL1 SGDID:S000000498, Chr II from 789230-791809, Verified ORF, "High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates" * Jamie_Ashy_SPB_97.34716.34716.3 2.5802 0.1604 15.1 3183.9243 3180.559 8091.1 19 4.308 19.2 1 I.IDYHRSCNVYTFSDGLRYVIVGPPPPR.V * Jamie_Ashy_SPB_15.05352.05352.2 1.8187 0.0572 11.8 1774.4722 1776.132 2834.5 277 3.109 36.7 1 T.ISWSIATFVVIVVANR.M YBR131W 2 2 5.0% 704 80732 5.3 U CCZ1 SGDID:S000000335, Chr II from 497157-499271, Verified ORF, "Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway" * Jamie_Ashy_SPB_31.11059.11059.3 2.6537 0.116 19.2 2376.0842 2379.576 7158.5 33 4.048 29.2 1 R.DYLKDLSFQLCQSMECLNN.E * Jamie_Ashy_SPB_22.07641.07641.2 1.8152 0.0908 14.7 1905.6721 1907.1018 6268.2 4 3.924 40.0 1 N.WFQNNGTSKAAKQRNR.F YHR023W 3 3 4.9% 1928 223634 6.4 U MYO1 SGDID:S000001065, Chr VIII from 151659-157445, Verified ORF, "Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively" * Jamie_Ashy_SPB_35.12561.12561.3 2.3045 0.1586 15.4 4461.9844 4463.093 7137.0 70 4.204 15.1 1 R.SRLKNGFILKHYAGDVEYTVEGWLSKNKDPLNDNLLSLL.S * Jamie_Ashy_SPB_67.23847.23847.2 1.8315 0.1635 33.5 1620.4922 1618.8307 9163.8 56 4.545 38.5 1 Q.KKRNSLVESLNDSK.I * Jamie_Ashy_SPB_85.30607.30607.4 2.9514 0.1442 11.5 4910.8564 4915.379 8419.4 178 3.475 13.4 1 G.NSVETNISSTRRSTSYSDDPLDKEDIIKKYYDLQLAFTEITR.N Reverse_YIL173W 3 3 4.9% 1549 174427 5.3 U VTH1 SGDID:S000001435, Chr IX from 11492-16141, Verified ORF, "Putative membrane glycoprotein with strong similarity to Vth2p and Pep1p/Vps10p, may be involved in vacuolar protein sorting" Reverse_YJL222W 3 3 4.9% 1549 174401 5.3 U VTH2 SGDID:S000003758, Chr X from 11475-16124, Verified ORF, "Putative membrane glycoprotein with strong similarity to Vth1p and Pep1p/Vps10p, may be involved in vacuolar protein sorting" Jamie_Ashy_SPB_99.35475.35475.2 1.6118 0.1072 11.8 1993.4122 1994.1681 10232.7 164 3.624 31.2 1 D.DQFFDTSSVLSRTQSFK.E Jamie_Ashy_SPB_70.25184.25184.4 2.836 0.0878 78.0 4274.3765 4271.7754 6286.7 212 3.004 15.3 1 R.DHTLFINGKSGFLYASSNFYPNFVIEVIKEGDTDFRK.I Jamie_Ashy_SPB_41.14769.14769.2 1.7201 0.0564 10.9 2243.7722 2241.5469 7817.0 16 3.576 28.6 1 E.TISTGTLIFKSGSGDSITSVLR.Y YKL101W 3 3 4.9% 1518 169592 9.3 U HSL1 SGDID:S000001584, Chr XI from 248564-253120, Verified ORF, "Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p" * Jamie_Ashy_SPB_103.36997.36997.3 1.1937 0.0028 32.7 4234.344 4236.0684 3930.0 13 2.551 8.3 1 R.DTVGPWKLGKTLGKGSSGRVRLAKNMETGQLAAIKIVPKK.K * Jamie_Ashy_SPB_41.14703.14703.4 2.6848 0.1491 15.1 5818.5767 5821.743 8803.8 22 3.711 11.7 1 K.LGKTLGKGSSGRVRLAKNMETGQLAAIKIVPKKKAFVHCSNNGTVPNSYSSSMVT.S * Jamie_Ashy_SPB_7.02372.02372.2 1.7589 0.1625 29.1 1342.3322 1339.5927 4029.1 265 3.692 40.9 1 A.LGISQSLQSMFK.D YLR398C 4 4 4.9% 1287 146058 6.7 U SKI2 SGDID:S000004390, Chr XII from 919019-915156, reverse complement, Verified ORF, "Putative RNA helicase, involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski3p and Ski8p; required for repressing propagation of dsRNA viruses" * Jamie_Ashy_SPB_51.18369.18369.3 1.997 0.0476 39.3 2729.4543 2733.2012 6641.1 333 2.751 21.6 1 R.NMTKTIYTSPIKALSNQKFRDFK.E * Jamie_Ashy_SPB_78.28003.28003.2 1.564 0.0923 13.5 1842.6522 1845.1027 5407.4 11 3.37 33.3 1 K.KELIPVINQNSEFLEA.N * Jamie_Ashy_SPB_103.36774.36774.2 1.6361 0.0747 12.8 1745.9922 1744.8304 5677.4 407 3.278 34.4 1 T.RDGRGGRGNSTRGGANR.G * Jamie_Ashy_SPB_12.04148.04148.1 2.7514 0.0555 60.0 867.24 867.8877 3170.2 36 2.775 75.0 1 K.DTEFIDQ.N YBL007C 2 2 4.9% 1244 135848 5.9 U SLA1 SGDID:S000000103, Chr II from 216369-212635, reverse complement, Verified ORF, "Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; contains 3 SH3 domains; interacts with proteins regulating actin dynamics and with proteins required for endocytosis" * Jamie_Ashy_SPB_11.03939.03939.4 1.6216 0.0281 33.3 4506.6567 4503.9272 6150.5 1 2.962 13.0 1 R.SRLQEKELPPIKPPRPTSTTSVPNTTSVPPAESSNNNNSSNK.Y * Jamie_Ashy_SPB_57.20550.20550.4 3.4071 0.1154 10.1 4496.2964 4498.668 7762.8 134 3.531 15.8 1 V.PNTTSVPPAESSNNNNSSNKYDWFEFFLNCGVDVSNCQR.Y YBR295W 2 2 4.9% 1216 131839 7.5 U PCA1 SGDID:S000000499, Chr II from 792843-796493, Verified ORF, "P-type metal-transporting ATPase with a role in copper and iron homeostasis; R970G-substitution in the C-terminal region confers cadmium resistance" * Jamie_Ashy_SPB_38.13562.13562.4 3.5871 0.0954 11.3 3970.1365 3973.2024 7251.9 45 3.715 16.7 1 K.LFSGLGTSDGEYGVVNSENISIDAMQDNRGECHRRS.I * Jamie_Ashy_SPB_47.16658.16658.4 3.0532 0.0888 16.4 2810.0566 2804.1448 6054.3 6 4.018 24.6 1 A.SKKTKYSYRNSSGCLTKKKTHGDK.E YNL192W 2 2 4.9% 1131 129919 6.2 U CHS1 SGDID:S000005136, Chr XIV from 276503-279898, Verified ORF, "Chitin synthase I, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor" * Jamie_Ashy_SPB_25.08816.08816.4 3.638 0.0338 11.4 3201.0166 3200.539 5066.9 106 4.073 20.4 1 S.KINERSLALLSSLGCYQDGFAKDEINEK.K * Jamie_Ashy_SPB_56.20144.20144.4 2.8536 0.1355 15.7 3202.6565 3197.5078 5687.8 365 3.812 20.5 1 W.LNGSFFASVYSFCHFYRVWSSGHNIGR.K YNL014W 2 2 4.9% 1044 115869 6.2 U HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells" * Jamie_Ashy_SPB_25.08792.08792.2 1.893 0.0211 10.8 1493.7922 1494.7925 4675.0 458 3.019 37.5 1 K.QKAIVKVSNMTFQ.Y * Jamie_Ashy_SPB_61.21892.21892.3 2.6815 0.1214 82.2 4424.0044 4426.9785 7343.2 1 3.934 17.6 1 R.ETMDRANRQINENDAEAMNKIFKIEGTPRRVAGIHSRR.K contaminant_UBIQUITIN03 2 2 4.9% 1011 111720 5.9 U no description * Jamie_Ashy_SPB_36.12764.12764.4 3.1561 0.1179 12.3 3108.9365 3108.6458 4787.7 189 3.212 19.0 1 R.AVQVVVHTGDITEDLLLDFQVVVLTAAKL.E * Jamie_Ashy_SPB_53.18834.18834.3 3.3073 0.2368 86.5 2309.4243 2310.7043 6574.9 18 4.478 30.0 1 R.VRILLHGSALLYAAGWSPEKQ.A YKL157W 2 2 4.9% 935 105585 7.9 U APE2 SGDID:S000001640, Chr XI from 154996-155276,155660-158186, Verified ORF, "Zinc-dependent metallopeptidase yscII, may have a role in obtaining leucine from dipeptide substrates; sequence coordinates have changed since RT-PCR analysis showed that the adjacent ORF YKL158W comprises the 5' exon of APE2/YKL157W" * Jamie_Ashy_SPB_32.11498.11498.3 2.199 0.0149 37.8 3413.2444 3416.9375 6856.9 137 3.405 17.5 1 K.GTMSSFKGNAFLDIKFTGILNDNMAGFYRAK.Y * Jamie_Ashy_SPB_74.26355.26355.2 1.6234 0.1121 15.0 1681.3121 1680.9751 6280.3 35 3.551 35.7 1 T.QRLKVTMFGAACAAR.D YER068W 2 2 4.9% 587 65354 8.1 U MOT2 SGDID:S000000870, Chr V from 293048-294811, Verified ORF, "Component of the CCR4-NOT transcription regulatory complex, which represses transcription, at least in part, by inhibiting functional TBP-DNA interactions and also aids in transcription elongation; interacts with C-terminal region of Not1p" * Jamie_Ashy_SPB_56.20154.20154.2 2.427 0.0173 15.8 1859.7722 1860.2255 7863.1 2 2.866 50.0 1 P.EELKMERAKLARKEK.E * Jamie_Ashy_SPB_15.05104.05104.2 1.8669 0.0942 15.3 1861.1322 1862.0121 4507.4 458 3.107 34.6 1 R.EKERKENEYTNRKH.L contaminant_KERATIN15 3 4 4.9% 629 64511 6.5 U no description Jamie_Ashy_SPB_5.01832.01832.2 2.1983 0.1982 81.2 1194.6921 1195.3176 4523.2 20 4.61 75.0 1 K.KYEDEINKR.Y * Jamie_Ashy_SPB_27.09644.09644.2 1.6723 0.0653 13.4 1134.7722 1134.3177 4375.4 3 3.454 68.8 1 A.LIDEIDFLR.T * Jamie_Ashy_SPB_45.15895.15895.2 2.5689 0.18 79.6 1303.2122 1303.4521 5351.7 10 5.473 58.3 2 R.SLDLDSIIAEVGA.Q YER129W 3 3 4.8% 1142 126872 8.7 U SAK1 SGDID:S000000931, Chr V from 417277-420705, Verified ORF, "Upstream kinase for the SNF1 complex; partially redundant function with Elm1p and Tos3p; members of this family of kinases have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome" * Jamie_Ashy_SPB_32.11409.11409.3 3.1799 0.1207 13.5 2569.4944 2567.1313 5552.1 326 3.907 25.0 1 H.ELKNAVSGVGKKIKESVLKSIPLK.D * Jamie_Ashy_SPB_66.23503.23503.2 1.5226 0.1098 12.2 2507.2522 2508.719 6950.3 23 3.42 23.8 1 R.PVLPQQKSSFCATGRYDKSHNS.L * Jamie_Ashy_SPB_14.05036.05036.2 1.1899 0.0603 62.2 1140.0521 1142.2999 5884.6 24 3.007 56.2 1 K.NFDKVLYSR.D YKL092C 2 2 4.8% 1104 126663 8.8 U BUD2 SGDID:S000001575, Chr XI from 269103-265789, reverse complement, Verified ORF, "GTPase activating factor for Rsr1p/Bud1p required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types" * Jamie_Ashy_SPB_12.04122.04122.3 3.0611 0.1435 11.0 2151.2344 2150.4368 6126.1 20 4.01 27.8 1 S.IDELTADSTNIQPIIKHLQ.Q * Jamie_Ashy_SPB_69.24716.24716.4 3.3103 0.1154 11.2 3908.4165 3911.5928 6865.0 186 4.085 18.2 1 I.LNLSSTISRPKLAIEQTMLDDLPQIPYLLDKNLR.E YPR042C 2 2 4.8% 1075 119508 8.8 U PUF2 SGDID:S000006246, Chr XVI from 653659-650432, reverse complement, Verified ORF, "Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins" * Jamie_Ashy_SPB_3.00912.00912.4 2.5732 0.0688 67.1 3304.3765 3305.7153 5106.3 377 3.535 17.8 1 K.LHHSKKSTPVVVVPPTSSTPDSLNSTTYAPR.V * Jamie_Ashy_SPB_49.17672.17672.2 1.9993 0.0252 13.6 2228.4722 2228.4329 8732.2 439 3.497 22.5 1 R.ARGVSVSSVRSSNSRHNSVIQ.M YMR047C 2 2 4.8% 1113 116234 9.3 U NUP116 SGDID:S000004650, Chr XIII from 366704-363363, reverse complement, Verified ORF, "Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a repetitive GLFG motif that interacts with mRNA export factor Mex67p and with karyopherin Kap95p; homologous to Nup100p" * Jamie_Ashy_SPB_67.24139.24139.3 2.516 0.2002 10.1 3660.1443 3663.8242 10373.6 62 4.65 16.2 1 P.ATGMFGTGTGSGGGFGSGATNSTGLFGSSTNLSGNSAFGAN.K * Jamie_Ashy_SPB_30.10812.10812.2 1.5938 0.0634 10.0 1373.4321 1372.666 5248.3 232 2.727 40.9 1 S.VMDKAQIKVDPK.L Reverse_YKR089C 2 2 4.8% 910 102716 8.0 U TGL4 SGDID:S000001797, Chr XI from 608007-605275, reverse complement, Verified ORF, "Triacylglycerol lipase involved in TAG mobilization; found in lipid particles; potential Cdc28p substrate" * Jamie_Ashy_SPB_118.42192.42192.2 1.5011 0.0987 12.5 2686.0723 2687.9316 8795.6 84 3.946 22.9 1 R.PNLSASTISYRPRFGSPSRGYTSAN.E * Jamie_Ashy_SPB_59.21067.21067.3 2.4834 0.2535 50.0 2235.7444 2235.6062 5788.7 62 4.662 31.9 1 Y.AERFTLDGLFEIMTNVLHK.N Reverse_YPL248C 3 3 4.8% 881 99403 7.2 U GAL4 SGDID:S000006169, Chr XVI from 82356-79711, reverse complement, Verified ORF, "DNA-binding transcription factor required for the activation of the GAL genes in response to galactose; repressed by Gal80p and activated by Gal3p" * Jamie_Ashy_SPB_20.07080.07080.2 1.5045 0.1601 10.4 975.0122 975.0898 3563.4 429 3.568 56.2 1 Y.ITPGTTTRQ.V * Jamie_Ashy_SPB_28.09840.09840.4 3.4467 0.1074 12.0 3634.4565 3632.9414 8095.1 219 3.257 17.7 1 R.QGKNSSEESSSTASIRHQRLTLPMDTEVSALRD.T * Jamie_Ashy_SPB_24.08523.08523.2 1.4534 0.0053 61.5 1447.0721 1446.702 6294.3 313 2.512 45.8 1 R.LTLPMDTEVSALR.D YMR089C 2 2 4.8% 825 93276 7.6 U YTA12 SGDID:S000004695, Chr XIII from 448085-445608, reverse complement, Verified ORF, "Component, with Afg3p, of the mitochondrial inner membrane m-AAA protease that mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes" * Jamie_Ashy_SPB_33.11659.11659.2 1.8528 0.1065 17.8 1543.5521 1544.7483 5895.6 11 2.967 54.2 1 I.DESIRRLEDAVLK.Q * Jamie_Ashy_SPB_75.26961.26961.3 2.8472 0.185 17.2 2962.2844 2962.3354 6718.8 55 3.927 21.2 1 K.TARENAPSIVFIDEIDAIGKARQKGNF.S Reverse_contaminant_PIGR_RAT 2 2 4.8% 769 84798 5.2 U owl|P15083| POLYMERIC-IMMUNOGLOBULIN RECEPTOR PRECURSOR (PLGR) (CONTAINS:... * Jamie_Ashy_SPB_22.07653.07653.3 2.1073 0.011 41.5 1396.6444 1399.5212 3087.4 215 2.968 34.1 1 R.SNRFDGMSIDTR.Y * Jamie_Ashy_SPB_57.20384.20384.2 1.4283 0.1357 12.3 2834.8523 2834.9856 8499.5 14 3.852 22.9 1 P.DLNAKNEDERVSSEMAEEEPADKAR.A Reverse_YKR093W 2 2 4.8% 601 68044 5.4 U PTR2 SGDID:S000001801, Chr XI from 615372-617177, Verified ORF, "Integral membrane peptide transporter, mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by the N-end rule pathway via repression by Cup9p" * Jamie_Ashy_SPB_22.07676.07676.2 1.9158 0.0551 16.3 1435.8722 1437.7344 5610.0 28 2.947 55.0 1 D.FKNKTLIWCVK.F * Jamie_Ashy_SPB_70.24942.24942.2 1.6582 0.0879 80.9 2036.1122 2033.286 9073.4 32 3.287 32.4 1 K.LTVAQTKEEEIVPFDGQK.E Reverse_YDR180W 3 3 4.7% 1493 171101 6.8 U SCC2 SGDID:S000002588, Chr IV from 821290-825771, Verified ORF, "Putative protein kinase and subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX" * Jamie_Ashy_SPB_105.37606.37606.3 1.5727 0.0311 33.5 4133.1846 4129.9033 9057.8 48 3.229 15.4 1 K.DLLHLVHRSNFLKPHNGCLKTLNKVAVRRIVHTIK.D * Jamie_Ashy_SPB_47.16885.16885.2 1.9385 0.1722 38.3 2017.7922 2018.3575 5904.1 249 3.716 31.2 1 Q.FTDFEIKLSGYEITLIK.L * Jamie_Ashy_SPB_98.35053.35053.2 1.5544 0.1193 12.8 2194.132 2194.4265 10332.8 3 3.825 32.4 1 L.FNVPEMIYSERHLNSDNK.G YDR359C 2 2 4.7% 982 112502 9.0 U VID21 SGDID:S000002767, Chr IV from 1194875-1191927, reverse complement, Verified ORF, "Component of the NuA4 histone acetyltransferase complex" * Jamie_Ashy_SPB_73.26196.26196.2 1.6113 0.1355 16.4 1391.8722 1390.7086 4762.3 114 3.801 45.5 1 K.KELAPPFKLSIF.V * Jamie_Ashy_SPB_90.32318.32318.4 3.3873 0.1608 10.5 3999.2966 4000.559 5270.0 1 4.425 21.7 1 T.EQDIIESYSRKLLEQKPDIGPEMALKAAKNYYRT.L Reverse_YOR211C 2 2 4.7% 902 101523 8.0 U MGM1 SGDID:S000005737, Chr XV from 741632-738924, reverse complement, Verified ORF, "Mitochondrial GTPase related to dynamin, present in a complex containing Ugo1p and Fzo1p; required for normal morphology of cristae and for stability of Tim11p; homolog of human OPA1 involved in autosomal dominant optic atrophy" * Jamie_Ashy_SPB_39.14068.14068.2 1.8398 0.0743 14.0 1863.4922 1862.2383 5749.7 171 3.705 32.4 1 T.SLRGIGMKTLGSSALELK.K * Jamie_Ashy_SPB_73.26211.26211.2 1.7617 0.2691 71.9 2684.9922 2686.0696 7804.1 22 5.076 23.9 1 R.TIVFSLANAHASRHVNSMRSHLHV.L YOR112W 2 2 4.7% 761 84769 5.4 U YOR112W SGDID:S000005638, Chr XV from 531508-533793, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_89.31996.31996.2 1.5678 0.1491 15.3 2920.152 2917.3027 7375.5 347 4.194 22.9 1 -.MNFSSIFKSISNFQFPYTIEETAIT.E * Jamie_Ashy_SPB_39.13898.13898.2 1.4418 0.1174 12.9 1156.9722 1157.3555 4180.4 90 3.664 50.0 1 K.TTILAPRNIAS.N YMR179W 2 2 4.7% 758 84697 6.0 U SPT21 SGDID:S000004791, Chr XIII from 619857-622133, Verified ORF, "Protein required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p" * Jamie_Ashy_SPB_28.10059.10059.2 1.7094 0.1122 15.4 2192.4922 2191.4495 5555.5 14 3.345 32.5 1 K.QTIVKVKNSNSKNSAKSTQAG.C * Jamie_Ashy_SPB_19.06681.06681.2 2.3452 0.0611 17.2 1589.9321 1587.73 5119.3 119 2.829 46.4 1 S.PTSASASQLNQQNLK.P Reverse_YBR260C 2 2 4.7% 666 74619 8.3 U RGD1 SGDID:S000000464, Chr II from 734634-732634, reverse complement, Verified ORF, "GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization" * Jamie_Ashy_SPB_49.17398.17398.2 1.189 0.0168 60.3 1328.9722 1326.4949 4403.6 196 2.89 45.0 1 K.RYIGEQDLGFK.D * Jamie_Ashy_SPB_21.07382.07382.2 1.3923 0.1183 11.9 2264.7322 2267.6394 6732.5 12 3.402 26.3 1 M.RKPISNRSPDVAFKPPSKNK.Q YNL039W 2 2 4.7% 594 67688 6.9 U BDP1 SGDID:S000004984, Chr XIV from 555049-556833, Verified ORF, "Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs" * Jamie_Ashy_SPB_11.04005.04005.2 1.825 0.0381 10.3 1661.8522 1660.9602 6291.5 438 3.06 42.9 1 S.SIVNKSGTRFAPKVR.Q * Jamie_Ashy_SPB_17.05851.05851.2 2.0968 0.0963 22.2 1464.7322 1464.6616 5919.1 3 3.418 54.2 1 I.KKNIGTVADFNEK.L Reverse_YDR323C 2 2 4.7% 515 59443 8.4 U PEP7 SGDID:S000002731, Chr IV from 1114022-1112475, reverse complement, Verified ORF, "Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance" * Jamie_Ashy_SPB_3.00948.00948.2 2.9368 0.0209 95.8 1717.4922 1717.87 6785.7 79 3.239 46.2 1 K.YDQITSEILYSQEK.L * Jamie_Ashy_SPB_54.19372.19372.2 1.6678 0.1537 24.4 1190.6721 1188.4587 4409.0 1 3.763 61.1 1 T.ILGDVLRVFR.N Reverse_YCR045C 2 2 4.7% 491 55068 5.4 U YCR045C SGDID:S000000641, Chr III from 209605-208130, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_82.29335.29335.2 1.6839 0.0833 12.0 1842.9521 1841.0734 6450.9 4 3.576 36.7 1 K.DVGNYLIQNPTSPKFF.L * Jamie_Ashy_SPB_1.00345.00345.1 1.2137 0.1872 12.5 797.34 797.7997 2551.9 123 3.992 50.0 1 R.NSNSTFQ.Q YMR109W 2 2 4.6% 1219 136899 9.3 U MYO5 SGDID:S000004715, Chr XIII from 486586-490245, Verified ORF, "One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization" Jamie_Ashy_SPB_115.41116.41116.2 1.6241 0.1591 10.6 2194.0923 2191.4575 5412.4 31 4.166 29.4 1 T.LRNNNSSRHGKYLEIKFN.N * Jamie_Ashy_SPB_57.20332.20332.3 2.0189 0.0473 39.2 3745.6743 3742.1436 7221.2 3 3.581 16.9 1 K.PVLNSVQHDNTSANVIPAAAQASLGDGLANALAARANK.M YBR023C 2 2 4.6% 1165 131601 6.7 U CHS3 SGDID:S000000227, Chr II from 287925-284428, reverse complement, Verified ORF, "Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan" * Jamie_Ashy_SPB_7.02367.02367.4 2.5763 0.0356 54.2 2618.6565 2622.8193 4089.4 374 2.567 21.4 1 K.SNSSIPHDDDNNLAWYFPCKLK.N * Jamie_Ashy_SPB_60.21469.21469.4 3.0518 0.1377 90.2 3694.2566 3690.373 9071.6 158 3.613 18.8 1 K.APLKEVDPHLRPKKYSKKSLGHKRASTFDLLK.K Reverse_YKL165C 2 2 4.6% 919 105693 6.7 U MCD4 SGDID:S000001648, Chr XI from 140696-137937, reverse complement, Verified ORF, "Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes" * Jamie_Ashy_SPB_44.15840.15840.2 2.0598 0.094 17.5 1983.5521 1983.2085 7518.4 2 4.069 36.1 1 D.GHSGFASMGHDATFIFATK.D * Jamie_Ashy_SPB_101.36236.36236.2 1.4833 0.2522 43.4 2476.8523 2479.8157 8020.1 51 4.552 25.0 1 K.WGKTVASVDEYFGAIMAVHGPRS.E Reverse_YJL165C 2 2 4.6% 855 95455 8.4 U HAL5 SGDID:S000003701, Chr X from 109454-106887, reverse complement, Verified ORF, "Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters" * Jamie_Ashy_SPB_70.25023.25023.4 3.1544 0.1395 13.8 2612.0964 2617.885 4415.9 16 3.958 24.6 1 Q.KNSNSRGEGNNGKTNESKLGKRIK.E * Jamie_Ashy_SPB_58.20646.20646.2 1.7787 0.0804 12.2 1844.5521 1845.0226 7325.5 286 3.721 32.1 1 K.ARAKEEKKNQLDERE.L YMR049C 2 2 4.6% 807 91705 5.2 U ERB1 SGDID:S000004652, Chr XIII from 370516-368093, reverse complement, Verified ORF, "Protein required for maturation of the 25S and 5.8S ribosomal RNAs; constituent of 66S pre-ribosomal particles; homologous to mammalian Bop1" * Jamie_Ashy_SPB_47.16771.16771.2 1.7881 0.134 10.2 1909.0721 1910.9856 6454.1 215 3.487 30.0 1 T.NEESYNPPEEYLLSPE.E * Jamie_Ashy_SPB_106.38170.38170.2 1.3767 0.0928 69.8 2223.6921 2223.139 5729.8 296 3.126 22.5 1 K.SNLEVNENGDGDEDGENESAK.N YDL227C 2 2 4.6% 586 66089 9.2 U HO SGDID:S000002386, Chr IV from 48032-46272, reverse complement, Verified ORF, "Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p" * Jamie_Ashy_SPB_91.32790.32790.2 1.3521 0.0099 61.7 1659.1921 1656.8447 6522.1 73 3.15 34.6 1 R.AFEGEPGRLDPRRR.T * Jamie_Ashy_SPB_10.03524.03524.2 2.0702 0.0858 10.5 1527.0122 1527.7704 6211.2 6 3.94 45.8 1 Q.QCQTLDGRIIIIP.K YDR294C 2 2 4.6% 589 65566 8.0 U DPL1 SGDID:S000002702, Chr IV from 1052222-1050453, reverse complement, Verified ORF, "Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate" * Jamie_Ashy_SPB_4.01296.01296.2 2.3914 0.0165 16.3 1232.0721 1232.5089 3605.3 11 3.119 70.0 1 K.FINKNTILLVG.S * Jamie_Ashy_SPB_30.10790.10790.2 1.8163 0.1042 16.2 1597.4722 1594.7672 5337.8 201 3.208 33.3 1 P.AWTGGLYGSPTLAGSR.P Reverse_YNL271C 5 5 4.5% 1953 219701 6.5 U BNI1 SGDID:S000005215, Chr XIV from 135384-129523, reverse complement, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1" * Jamie_Ashy_SPB_56.19887.19887.4 2.9398 0.1011 16.5 3234.6165 3234.5164 5838.3 366 3.561 20.8 1 K.LLMRARDSIEEQDELASTKSPSPLDESTN.M * Jamie_Ashy_SPB_61.21847.21847.2 1.6821 0.1131 15.4 1606.7922 1609.4675 6527.3 185 4.114 35.7 1 R.DEAEDEENGESSPSN.S * Jamie_Ashy_SPB_93.33526.33526.2 1.6177 0.1047 12.5 2119.5322 2121.2249 7279.4 428 3.368 22.5 1 Q.SLADLFSGPGSGSSLESHESR.L * Jamie_Ashy_SPB_4.01212.01212.3 2.1338 0.1662 18.5 1737.3544 1736.0677 4661.9 10 3.63 33.9 1 N.LKNVDAEQPKVLPKR.D * Jamie_Ashy_SPB_9.03206.03206.2 2.064 0.0079 86.2 832.7922 832.0311 3571.0 36 2.271 78.6 1 K.KLSGSVLK.K YLR342W 3 3 4.5% 1876 214849 7.2 U FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling" * Jamie_Ashy_SPB_5.01738.01738.4 1.0789 0.0438 32.8 4896.7764 4899.42 3656.0 307 3.035 5.2 1 R.SSRMSPDQALLSLHADYIGGDTANYKKWYFAAQLDMDDEIGFR.N * Jamie_Ashy_SPB_81.29065.29065.2 1.6004 0.1114 13.1 1814.7722 1815.165 5223.3 4 3.787 36.7 1 I.FGINLGPIIFVFAYDK.D Jamie_Ashy_SPB_60.21316.21316.4 2.4684 0.111 75.7 2782.9365 2778.1606 5792.8 434 3.4 25.0 1 K.LHYGHPDFINATFMTTRGGVSKAQK.G YBR097W 4 4 4.5% 1454 166358 6.7 U VPS15 SGDID:S000000301, Chr II from 436945-441309, Verified ORF, "Myristoylated serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; also detected in mitochondria" * Jamie_Ashy_SPB_5.01618.01618.2 1.6074 0.0766 14.8 816.2522 816.0347 2634.7 45 2.868 75.0 1 F.KTGLIRK.K * Jamie_Ashy_SPB_15.05244.05244.2 1.8805 0.0766 15.4 1497.0122 1498.6792 5307.3 17 3.512 45.8 1 G.FNKRDTKSSSSLK.G * Jamie_Ashy_SPB_34.12062.12062.2 1.8587 0.0669 12.3 2040.5521 2038.3518 6178.7 3 3.079 36.1 1 I.NGSLIFKNKSIATTTSNLK.N * Jamie_Ashy_SPB_109.39144.39144.3 1.6905 0.114 43.2 3208.9143 3209.6 4498.3 104 3.625 16.3 1 R.FNVLIRSWSFGDHAPITHVEVCQFYGK.N YER105C 3 3 4.5% 1391 156649 6.4 U NUP157 SGDID:S000000907, Chr V from 372009-367834, reverse complement, Verified ORF, "Abundant subunit of the nuclear pore complex (NPC), present on both sides of the NPC, has similarity to Nup170p" * Jamie_Ashy_SPB_93.33337.33337.2 2.1609 0.0713 17.7 2051.892 2050.1953 6354.5 5 3.71 44.1 1 D.DQNNGKPNLSSRSFLSER.K * Jamie_Ashy_SPB_10.03374.03374.2 1.6767 0.0393 10.0 1177.5122 1175.284 4478.3 292 3.003 50.0 1 T.ISQLEVDQSR.G * Jamie_Ashy_SPB_48.17050.17050.4 3.5041 0.1519 11.4 3875.0964 3870.3113 6753.3 285 3.763 16.2 1 Y.SAEPLKVAVLTSNALEIYCYRTPDEVFESLIENP.L YGL167C 2 2 4.5% 950 104571 5.7 U PMR1 SGDID:S000003135, Chr VII from 190474-187622, reverse complement, Verified ORF, "High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease" * Jamie_Ashy_SPB_88.31483.31483.2 1.3731 0.1791 16.1 2610.0522 2609.0222 7794.5 275 3.828 23.8 1 K.LWCLDSMSNKLNVLSLDKNKKT.K * Jamie_Ashy_SPB_14.04879.04879.4 2.5132 0.1658 13.9 2400.0964 2405.7996 8433.2 200 3.651 25.0 1 Y.VKGAFERILEYSTSYLKSKGK.K Reverse_YBL008W 2 2 4.5% 840 93890 7.2 U HIR1 SGDID:S000000104, Chr II from 209656-212178, Verified ORF, "Non-essential transcriptional corepressor involved in the cell cycle-regulated transcription of histone H2A, H2B, H3 and H4 genes; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores" * Jamie_Ashy_SPB_80.28549.28549.2 1.6871 0.0325 71.1 1556.7122 1557.7552 4383.6 37 3.209 45.5 1 K.YLSWCGLDKTWK.Y * Jamie_Ashy_SPB_52.18673.18673.3 2.853 0.1266 17.1 2674.9744 2679.1326 7268.1 291 3.144 21.0 1 N.SFPSSSSTSILTPAVRKKGSKLTVAK.N Reverse_YFL023W 3 3 4.5% 796 91422 4.7 U BUD27 SGDID:S000001871, Chr VI from 90984-93374, Verified ORF, "Protein involved in bud-site selection, nutrient signaling, and gene expression controlled by the TOR kinase; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern" * Jamie_Ashy_SPB_37.13261.13261.2 2.0676 0.0533 89.4 1435.2322 1435.635 6838.2 313 3.105 55.0 1 R.FRSVRTRRNSR.S * Jamie_Ashy_SPB_44.15829.15829.2 1.887 0.0686 14.2 1824.7122 1827.0441 7305.3 33 3.534 36.7 1 D.AFTYVNETDVKSPQVK.K * Jamie_Ashy_SPB_7.02239.02239.2 1.6096 0.0095 67.3 1005.3522 1004.08795 5694.2 108 2.838 62.5 1 K.KSNSQSPQK.T Reverse_YKR001C 2 2 4.5% 704 78737 7.9 U VPS1 SGDID:S000001709, Chr XI from 442365-440251, reverse complement, Verified ORF, "GTPase required for vacuolar protein sorting, functions in actin cytoskeleton organization via its interaction with Sla1p; required for late Golgi-retention of some proteins including Kex2p; involved in regulating peroxisome biogenesis" * Jamie_Ashy_SPB_50.17737.17737.2 1.8068 0.1054 12.9 1489.7522 1491.8577 5914.9 304 3.289 42.3 1 A.KLVPPPSELAALKK.K * Jamie_Ashy_SPB_38.13616.13616.3 2.6685 0.203 27.9 1808.3344 1810.0575 4448.9 3 3.94 35.3 1 K.SSSPQTPLPKGTKPDVAV.Q Reverse_YDL025C 2 2 4.5% 620 69615 8.3 U YDL025C SGDID:S000002183, Chr IV from 407203-405341, reverse complement, Uncharacterized ORF, "Protein of unknown function, potentially phosphorylated by Cdc28p" * Jamie_Ashy_SPB_118.42319.42319.2 1.8538 0.1624 32.4 1860.3322 1862.9011 11371.5 20 4.099 36.7 1 K.NPGRVIDDEDEPEETF.L * Jamie_Ashy_SPB_33.11743.11743.2 1.8172 0.0585 12.2 1422.6721 1421.4607 6424.9 324 2.938 45.5 1 Y.KIQDEQDSDTNK.K YGL228W 2 2 4.5% 577 66862 5.9 U SHE10 SGDID:S000003197, Chr VII from 67598-69331, Verified ORF, "Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest" * Jamie_Ashy_SPB_113.40377.40377.1 1.1683 0.017 78.5 874.67 874.07086 5264.6 5 3.18 57.1 1 K.VAIQPAFK.V * Jamie_Ashy_SPB_40.14234.14234.2 2.2736 0.069 10.6 2075.872 2074.298 7613.0 4 3.545 41.2 1 F.EKDVSKYINGKLVEEANH.F Reverse_YBR020W 2 2 4.5% 528 57944 6.7 U GAL1 SGDID:S000000224, Chr II from 279021-280607, Verified ORF, "Galactokinase, phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p" * Jamie_Ashy_SPB_30.10804.10804.3 2.4066 0.0389 54.8 1846.5844 1845.2512 5754.8 50 3.025 33.3 1 K.KNALSEEVLVLMKTLR.E * Jamie_Ashy_SPB_5.01692.01692.2 1.8819 0.0161 12.4 851.1722 850.9041 4168.6 25 2.228 71.4 1 G.KNTSSGEK.G Reverse_YLR163C 2 2 4.5% 462 51084 7.0 U MAS1 SGDID:S000004153, Chr XII from 493256-491868, reverse complement, Verified ORF, "Smaller subunit of the mitochondrial processing protease, essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins" * Jamie_Ashy_SPB_14.04994.04994.2 1.6039 0.0789 12.0 1174.5721 1174.2589 6966.6 309 3.225 55.0 1 H.ATGNNKVNEAR.S * Jamie_Ashy_SPB_11.03711.03711.2 1.9118 0.0743 16.4 1028.5922 1028.237 5517.2 3 3.427 77.8 1 E.TAITLGNPLK.S Reverse_YHR023W 4 4 4.4% 1928 223634 6.4 U MYO1 SGDID:S000001065, Chr VIII from 151659-157445, Verified ORF, "Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively" * Jamie_Ashy_SPB_103.36978.36978.3 1.709 0.0516 33.5 3510.7744 3509.8635 7419.1 126 2.857 17.9 1 K.MELEQQSIYKELDSIQAEYKLTAEEFESR.N * Jamie_Ashy_SPB_60.21386.21386.4 3.5735 0.134 21.7 3822.2966 3821.1584 7545.1 315 4.013 18.8 1 E.EYLDINKQKEDDFKDKYGNCSAQLTKVLEGNR.S * Jamie_Ashy_SPB_16.05593.05593.2 2.133 0.1987 60.9 1735.6921 1736.9658 5979.4 1 4.168 50.0 1 S.VADDRQKSFTALEKK.H * Jamie_Ashy_SPB_19.06673.06673.2 1.8587 0.0984 19.8 1009.6322 1008.0703 3216.1 246 3.708 56.2 1 D.LDEESSSLK.D YOR341W 2 2 4.4% 1664 186431 7.1 U RPA190 SGDID:S000005868, Chr XV from 960982-965976, Verified ORF, "RNA polymerase I subunit; largest subunit of RNA polymerase I" * Jamie_Ashy_SPB_74.26434.26434.4 2.5098 0.1659 15.7 5028.3364 5032.748 9609.8 269 3.687 12.7 1 H.YKQSVKYDPVLAKYNPAKYLGSVSENFQDKLESFLDKNSKLFK.S * Jamie_Ashy_SPB_49.17358.17358.3 2.873 0.1161 17.4 3383.3943 3385.5425 7011.7 134 3.95 18.1 1 V.AVPRLQTDVANSSSNSKRLEEDNDEEQSHK.K YCL014W 3 3 4.4% 1636 184718 6.2 U BUD3 SGDID:S000000520, Chr III from 96280-101190, Verified ORF, "Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding" * Jamie_Ashy_SPB_68.24360.24360.2 1.5299 0.097 12.7 2312.5122 2309.6335 8881.7 6 3.825 30.6 1 R.HCYIDEVLVSILDKSTLRF.D * Jamie_Ashy_SPB_92.32973.32973.4 2.902 0.1022 15.4 3603.5764 3599.3254 6112.9 127 3.288 19.4 1 P.VQRVPKKISQRHPKSPVKKPMTSEKKSSPKR.A * Jamie_Ashy_SPB_79.28422.28422.2 1.4382 0.1669 17.1 2589.9321 2589.911 10089.2 115 3.915 21.4 1 K.QKDEPIWVSPSKIDFADLSRRT.K Reverse_YJR033C 2 2 4.4% 1357 154932 8.2 U RAV1 SGDID:S000003794, Chr X from 496365-492292, reverse complement, Verified ORF, "Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate" * Jamie_Ashy_SPB_58.20614.20614.4 3.3063 0.1161 11.0 4352.5366 4350.054 6069.8 151 3.541 15.4 1 K.DFLSPRMRATSIPESAMAGIASYARDKTPSKLLKQVPISN.R * Jamie_Ashy_SPB_117.42157.42157.2 1.8298 0.1171 17.5 2170.5122 2171.542 10226.3 5 3.785 31.6 1 K.GISVILQSDQSIIVNYVPKP.Q YJL020C 3 3 4.4% 1157 128296 5.3 U BBC1 SGDID:S000003557, Chr X from 402326-398853, reverse complement, Verified ORF, "Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches" * Jamie_Ashy_SPB_40.14433.14433.2 1.6144 0.0833 13.0 1600.8322 1599.8889 5865.6 15 3.537 42.3 1 G.NGERKVPMDSPKLK.A * Jamie_Ashy_SPB_21.07539.07539.2 2.881 0.0251 26.2 1795.3522 1794.87 7496.5 17 3.523 50.0 1 I.TKTSREADEGTNDIEK.E * Jamie_Ashy_SPB_87.31318.31318.2 1.9051 0.0516 12.7 2037.5521 2039.316 6066.5 380 3.466 22.5 1 R.FGAPVGFNPFGMASGVGNKPS.E Reverse_YPL022W 2 2 4.4% 1100 126370 5.5 U RAD1 SGDID:S000005943, Chr XVI from 506695-509997, Verified ORF, "Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein" * Jamie_Ashy_SPB_87.31212.31212.4 3.0144 0.1212 15.4 3411.3765 3409.8271 5217.8 30 4.203 20.0 1 I.KSVGPVNLLRKFKSENDGDKLGKATSNFDSR.V * Jamie_Ashy_SPB_79.28482.28482.2 1.6793 0.093 11.3 2085.2522 2087.5 8869.7 46 3.295 31.2 1 R.LEKMKTRLPSFEMVFTE.P YJR035W 2 2 4.4% 1085 124527 7.5 U RAD26 SGDID:S000003796, Chr X from 497269-500526, Verified ORF, "Protein involved in transcription-coupled repair nucleotide excision repair of UV-induced DNA lesions; homolog of human CSB protein" * Jamie_Ashy_SPB_73.26294.26294.4 3.2851 0.102 15.0 3721.6565 3725.2954 5642.6 227 3.745 18.9 1 K.TALQRYVNKKNHLTRKLNNTTRISVKQNLRD.Q * Jamie_Ashy_SPB_76.27404.27404.2 1.8616 0.2045 49.5 1807.4922 1805.1735 5951.6 6 3.921 37.5 1 K.TIQVIAFIAALHHSGLL.T Reverse_YIL156W 3 3 4.4% 1071 123134 7.9 U UBP7 SGDID:S000001418, Chr IX from 48091-51306, Verified ORF, "Ubiquitin-specific protease that cleaves ubiquitin-protein fusions" * Jamie_Ashy_SPB_31.11157.11157.2 1.7391 0.1381 25.5 1133.6921 1133.2462 6197.7 31 3.876 56.2 1 R.QPQLIQQDY.H * Jamie_Ashy_SPB_47.16726.16726.4 3.5133 0.1525 27.0 4420.2163 4419.725 7285.4 412 3.517 15.3 1 P.PEPVKTSSEKASFSDQSDPQQQQQQQEQQLRAKQSYPR.Y * Jamie_Ashy_SPB_33.11823.11823.2 2.0841 0.0248 13.1 1782.3121 1782.8674 7265.9 155 3.113 38.5 1 S.DPQQQQQQQEQQLR.A Reverse_YOL089C 2 2 4.4% 1030 117926 7.7 U HAL9 SGDID:S000005449, Chr XV from 153489-150397, reverse complement, Verified ORF, "Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression" * Jamie_Ashy_SPB_70.25062.25062.2 1.7453 0.0335 71.3 1774.1721 1774.8845 8535.7 1 3.069 37.5 1 R.NSSPSLDGFNSIPSHSK.L * Jamie_Ashy_SPB_14.04940.04940.3 2.0392 0.0673 43.2 3259.0144 3254.6301 6687.8 8 3.434 21.3 1 R.SCAIEFSLAPNEQSKQAYLVSLYQKYSK.L Reverse_YDR270W 2 2 4.4% 1004 109829 5.2 U CCC2 SGDID:S000002678, Chr IV from 1005669-1008683, Verified ORF, "Cu(+2)-transporting P-type ATPase, required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases" * Jamie_Ashy_SPB_62.22344.22344.3 3.0076 0.098 17.7 3653.7244 3657.3027 8716.9 4 4.127 19.5 1 K.LFKKVVMFGTTLTGTKDFVFTTISNFKELVEGG.K * Jamie_Ashy_SPB_21.07491.07491.2 1.7969 0.0911 16.0 1212.8522 1213.4197 3929.9 299 4.016 45.0 1 G.QIPAKSLQAEK.M YMR205C 3 3 4.4% 959 104618 6.7 U PFK2 SGDID:S000004818, Chr XIII from 674765-671886, reverse complement, Verified ORF, "Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes" * Jamie_Ashy_SPB_111.39783.39783.2 1.2451 0.0047 60.2 2203.9722 2202.5085 6685.3 131 3.14 21.4 1 R.PQKAIAVMTSGGDAPGMNSNVR.A * Jamie_Ashy_SPB_24.08521.08521.2 1.6868 0.047 10.7 1284.0322 1284.4515 4919.9 68 3.131 55.0 1 A.TSADYIFIPEK.P * Jamie_Ashy_SPB_4.01230.01230.2 1.4981 0.093 12.4 947.59216 947.2089 6261.1 6 3.479 62.5 1 M.AIKAVGFIK.D YKL072W 2 2 4.4% 766 88836 8.6 U STB6 SGDID:S000001555, Chr XI from 299226-301526, Verified ORF, "Protein that binds Sin3p in a two-hybrid assay" * Jamie_Ashy_SPB_29.10226.10226.2 1.8122 0.2058 49.5 2339.7522 2339.6511 5548.5 27 4.043 31.0 1 S.NEILTTDLDTLVKNIHGGSLGK.L * Jamie_Ashy_SPB_16.05458.05458.2 2.1109 0.192 63.1 1543.2922 1543.7654 4673.7 4 3.625 50.0 1 T.FDTFLYKNRPNK.E Reverse_YHR178W 2 2 4.4% 743 83473 8.5 U STB5 SGDID:S000001221, Chr VIII from 459299-461530, Verified ORF, "Activator of multidrug resistance genes, forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a PDRE (pleotropic drug resistance element) in vitro; binds Sin3p in a two-hybrid assay" * Jamie_Ashy_SPB_34.12223.12223.2 1.3817 0.0213 61.5 2436.612 2437.6892 6705.7 19 2.803 25.0 1 R.MALWIESSHFSIVNTTSNNER.E * Jamie_Ashy_SPB_26.09307.09307.2 1.5909 0.1296 16.3 1392.0721 1392.5143 5273.0 15 3.424 45.5 1 G.DERVWSTTRGGK.F YOR140W 2 2 4.4% 766 83317 8.7 U SFL1 SGDID:S000005666, Chr XV from 586981-589281, Verified ORF, "Transcription repressor involved in regulation of flocculation-related genes, inhibits transcription by recruiting general corepressor Cyc8p-Tup1p to different promoters; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p" * Jamie_Ashy_SPB_41.14646.14646.3 2.4769 0.1211 13.8 2105.9644 2109.22 8193.9 6 3.511 34.7 1 R.KNSSNQNYDIDSGARVRPS.S * Jamie_Ashy_SPB_88.31430.31430.2 1.8193 0.2384 62.5 1537.6522 1534.7098 4544.6 397 4.571 32.1 1 K.PRNSLKSSISGTSLS.S Reverse_YGL099W 2 2 4.4% 640 72728 8.8 U LSG1 SGDID:S000003067, Chr VII from 314634-316556, Verified ORF, "Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm" * Jamie_Ashy_SPB_12.04155.04155.2 1.9671 0.1023 17.8 1619.6522 1620.8889 4722.5 1 3.436 53.8 1 P.PPNVYLLKGNVYDK.L * Jamie_Ashy_SPB_56.20144.20144.2 1.5965 0.0769 10.3 1601.8322 1600.9504 5687.8 199 3.402 38.5 1 R.HTPKPGKPAKWKKP.A YHR114W 2 2 4.4% 633 71171 6.0 U BZZ1 SGDID:S000001156, Chr VIII from 338086-339987, Verified ORF, "SH3 domain protein implicated in the regulation of actin polymerization, able to recruit actin polymerization machinery through its SH3 domains, colocalizes with cortical actin patches and Las17p, interacts with type I myosins" * Jamie_Ashy_SPB_103.36901.36901.2 1.2458 0.1154 69.1 2395.6921 2395.813 7035.9 13 3.547 34.2 1 K.QNKPSLNTAIFIKHNLKNWK.E * Jamie_Ashy_SPB_2.00662.00662.2 2.3133 0.1422 73.2 984.7322 985.1776 4070.0 5 3.709 78.6 1 P.RRSTLPVR.T YGL116W 2 2 4.4% 610 67360 8.8 U CDC20 SGDID:S000003084, Chr VII from 289812-291644, Verified ORF, "Cell-cycle regulated activator of anaphase-promoting complex/cyclosome (APC/C), which is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; potential Cdc28p substrate" * Jamie_Ashy_SPB_77.27409.27409.2 2.1692 0.2407 82.2 1319.4922 1322.4636 4435.0 84 4.705 54.5 1 L.QASANSIYSRPK.I * Jamie_Ashy_SPB_45.16178.16178.2 1.645 0.1661 20.4 1750.9321 1750.9956 7546.5 200 3.393 32.1 1 R.SGEIQINDVRIKQHI.V Reverse_YER068W 2 2 4.4% 587 65354 8.1 U MOT2 SGDID:S000000870, Chr V from 293048-294811, Verified ORF, "Component of the CCR4-NOT transcription regulatory complex, which represses transcription, at least in part, by inhibiting functional TBP-DNA interactions and also aids in transcription elongation; interacts with C-terminal region of Not1p" * Jamie_Ashy_SPB_102.36516.36516.2 1.6724 0.0732 12.8 1649.9122 1652.7671 5267.6 3 3.46 46.2 1 G.GSQQQAQQKNHLER.K * Jamie_Ashy_SPB_67.23857.23857.2 1.6757 0.1311 17.4 1590.7922 1588.7665 5996.1 323 3.883 45.5 1 S.LHKRNTYENEKR.E YPL100W 2 2 4.4% 496 55188 7.5 U ATG21 SGDID:S000006021, Chr XVI from 361869-363359, Verified ORF, "Phosphatidylinositol 3,5-bisphosphate-binding protein required for maturation of pro-aminopeptidase I, predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization" * Jamie_Ashy_SPB_51.18127.18127.2 1.6969 0.0223 69.2 1499.6322 1498.6855 6675.0 33 3.22 45.5 1 K.CFEIDTKNSKKK.T * Jamie_Ashy_SPB_13.04634.04634.2 1.9621 0.0986 21.8 1130.2722 1130.2522 7221.3 1 3.523 72.2 1 N.GSNPSVRTRR.N YNL237W 2 2 4.4% 459 51644 6.7 U YTP1 SGDID:S000005181, Chr XIV from 205189-206568, Verified ORF, "Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins" * Jamie_Ashy_SPB_29.10484.10484.2 1.67 0.0642 13.0 1485.9922 1486.5708 5634.2 269 2.96 45.8 1 E.HDMGMDDTSGYTR.P * Jamie_Ashy_SPB_5.01710.01710.2 1.3995 0.0054 63.5 944.53217 945.1123 2903.4 3 3.197 91.7 1 K.RLSYIHR.G YGL195W 5 5 4.3% 2672 296697 5.3 U GCN1 SGDID:S000003163, Chr VII from 131531-139549, Verified ORF, "Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA" * Jamie_Ashy_SPB_42.15111.15111.3 2.6344 0.1884 10.6 2271.4143 2273.6555 4815.0 80 3.742 26.3 1 S.EQKDPWQGRSTVAITLKIMA.K * Jamie_Ashy_SPB_101.36264.36264.4 3.0235 0.1037 85.8 4064.4966 4060.7917 7484.7 5 3.61 17.1 1 R.AVKEILPTLTGMIVTHLASSSNVLRNIAAQTLGDLVRR.V * Jamie_Ashy_SPB_21.07365.07365.1 1.5988 0.1579 13.3 1493.69 1494.5524 3271.2 156 3.735 32.1 1 V.DAIIGTSEDESTKGA.L * Jamie_Ashy_SPB_20.07112.07112.3 2.2008 0.0302 40.4 3704.5444 3704.1555 7482.2 371 3.429 16.7 1 R.GPNCVLPIFLHGLMYGSNDEREESALAIADVVSK.T * Jamie_Ashy_SPB_9.03261.03261.2 1.6548 0.0541 11.7 939.2522 938.1582 5359.8 341 3.05 71.4 1 L.RDPVIPIK.L Reverse_YOR290C 5 5 4.3% 1703 194050 7.0 U SNF2 SGDID:S000005816, Chr XV from 860255-855144, reverse complement, Verified ORF, "Catalytic subunit of the 11-subunit SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p" * Jamie_Ashy_SPB_64.22956.22956.3 2.7426 0.1209 17.1 2941.9443 2946.289 6528.0 4 3.229 25.0 1 E.YVVSGETNYIRANSFILHFDQLTEK.L * Jamie_Ashy_SPB_42.14916.14916.4 2.4342 0.1479 15.5 5116.4966 5121.799 6276.1 5 3.779 13.5 1 S.TAAAPTRVYDLGNRAKPPRGRGRGRGRGRGRGRGRGRTSTRASKVSTK.G * Jamie_Ashy_SPB_60.21513.21513.3 2.3414 0.0065 38.2 4040.5444 4037.5583 8667.2 1 3.464 19.4 1 R.VYDLGNRAKPPRGRGRGRGRGRGRGRGRGRTSTRASK.V * Jamie_Ashy_SPB_1.00296.00296.2 2.6601 0.1056 98.1 1860.8322 1862.1199 4914.3 41 3.681 40.6 1 K.PPRGRGRGRGRGRGRGR.G * Jamie_Ashy_SPB_37.13178.13178.2 1.5918 0.0202 65.1 1315.8922 1317.4567 5037.5 131 3.023 45.5 1 R.GRGRGRGRTSTR.A YGR109W-B 2 2 4.3% 1547 178419 8.8 U YGR109W-B SGDID:S000007347, Chr VII from 707614-708463,708465-712258, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" * Jamie_Ashy_SPB_54.19362.19362.3 2.9514 0.1985 27.4 3010.5244 3013.3835 7427.5 405 3.696 19.4 1 N.PRNIKLSFAPTILEATDPKSAGNRGDSR.T Jamie_Ashy_SPB_102.36614.36614.4 2.4799 0.0789 66.9 4108.5767 4109.6226 7709.5 18 3.051 17.6 1 R.TLGKSPFEIDLGYLPNTPAIKSDDEVNARSFTAVELAK.H YMR176W 2 2 4.3% 1411 162700 6.8 U ECM5 SGDID:S000004788, Chr XIII from 611739-615974, Verified ORF, "Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks" * Jamie_Ashy_SPB_95.33980.33980.3 2.9242 0.1632 10.5 3117.3542 3115.5867 7750.8 6 3.9 22.1 1 G.FNAVCEKKLWAQIGRELGYSGRIMSSL.S * Jamie_Ashy_SPB_83.29592.29592.4 3.4786 0.0871 15.1 3761.8965 3755.897 6050.4 60 3.639 19.8 1 K.SFIETDFYKSFLDAEQSADYSNTGDNSKNSFPE.D Reverse_YOR116C 3 3 4.3% 1460 162301 8.2 U RPO31 SGDID:S000005642, Chr XV from 544145-539763, reverse complement, Verified ORF, "RNA polymerase III subunit C160, part of core enzyme; similar to bacterial beta-prime subunit" * Jamie_Ashy_SPB_58.20908.20908.2 1.7399 0.078 12.8 1287.3522 1286.5143 4046.1 100 3.241 50.0 1 D.KGIINVDSAQIK.L * Jamie_Ashy_SPB_102.36766.36766.2 1.5608 0.0722 10.1 1540.1522 1537.8597 4992.4 43 3.382 37.5 1 K.GTWLYYPKMIAPP.P * Jamie_Ashy_SPB_37.13092.13092.4 3.0856 0.0888 16.3 4148.0967 4153.7764 6483.5 2 3.61 17.6 1 V.EKWEGVWIDKEPASKKGVWRFTDHIIKLAASGAGAAAK.K YIR006C 3 3 4.3% 1480 160266 5.3 U PAN1 SGDID:S000001445, Chr IX from 369905-365463, reverse complement, Verified ORF, "Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; previously thought to be a subunit of poly(A) ribonuclease" * Jamie_Ashy_SPB_34.12165.12165.2 1.75 0.0402 74.3 1584.9922 1583.8247 6629.5 57 3.455 38.5 1 K.EKPSFGKIDALSYK.N * Jamie_Ashy_SPB_79.28398.28398.2 1.7538 0.1623 26.1 1801.6322 1801.2504 5996.7 36 4.171 33.3 1 R.KKAKSRALLRARMSAL.T * Jamie_Ashy_SPB_30.10720.10720.4 3.636 0.1081 10.6 3463.6965 3464.9927 6932.7 86 3.241 17.7 1 D.NKVEEAKIGHPDHARAPPVTAAPLPSVTPVPPAV.P Reverse_YML049C 4 4 4.3% 1361 153784 5.8 U RSE1 SGDID:S000004513, Chr XIII from 178305-174220, reverse complement, Verified ORF, "Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport" * Jamie_Ashy_SPB_11.03945.03945.3 1.9281 0.0837 49.4 1775.0944 1777.8395 4225.7 7 3.307 35.0 1 R.GQLVVSGSEEEDDEKR.M * Jamie_Ashy_SPB_65.23126.23126.2 1.8288 0.1719 39.4 1256.1721 1253.3977 3315.9 87 3.611 50.0 1 D.KSTLVNSFDNK.E * Jamie_Ashy_SPB_54.19359.19359.2 1.8501 0.1249 10.3 1980.5122 1979.2648 6750.6 134 3.636 34.4 1 S.SLIICRGNPDIEMYSIP.S * Jamie_Ashy_SPB_92.33097.33097.2 1.6744 0.1581 19.1 1701.1721 1699.0613 6074.5 12 3.983 39.3 1 K.MSTITAFLNQFKAIL.K Reverse_YDL195W 2 2 4.3% 1273 138703 5.7 U SEC31 SGDID:S000002354, Chr IV from 107209-111030, Verified ORF, "Essential phosphoprotein component (p150) of the COPII coat of secretory pathway vesicles, in complex with Sec13p; required for ER-derived transport vesicle formation" * Jamie_Ashy_SPB_91.32517.32517.2 1.7601 0.0771 12.1 1626.1322 1626.8745 5433.6 41 3.579 42.3 1 K.MLAVLDHLCDITPQ.T * Jamie_Ashy_SPB_77.27588.27588.4 3.4996 0.2118 61.2 4305.7764 4311.7695 7184.2 354 4.071 15.4 1 K.AKLDWISAFNSSGASAFVHALSQNWALSIVEDVSSMSQGPT.L Reverse_YEL031W 2 2 4.3% 1215 135268 6.6 U SPF1 SGDID:S000000757, Chr V from 90258-93905, Verified ORF, "P-type ATPase, ion transporter of the ER membrane involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1" * Jamie_Ashy_SPB_51.18158.18158.3 2.6326 0.1923 10.8 2485.1042 2480.694 8021.6 4 3.742 24.0 1 L.GEETGNLLAIGVHAQKLAGVDNTGD.G * Jamie_Ashy_SPB_95.33987.33987.4 2.9236 0.1504 12.2 3204.7766 3202.5974 5491.4 449 3.906 21.8 1 L.ALVRSGSRTFSKYIEDYNKPIDSLRER.I Reverse_YHR079C 2 2 4.3% 1115 126976 6.6 U IRE1 SGDID:S000001121, Chr VIII from 261593-258246, reverse complement, Verified ORF, "Serine-threonine kinase and endoribonuclease; transmembrane protein that initiates the unfolded protein response signal by regulating synthesis of Hac1p through HAC1 mRNA splicing" * Jamie_Ashy_SPB_37.13352.13352.4 3.2131 0.1152 12.2 3320.1365 3318.8994 6806.6 25 3.146 22.8 1 A.DFKMLLASPPDRNEIELRDSVKLLFELK.K * Jamie_Ashy_SPB_30.10724.10724.2 2.1712 0.0369 17.0 2200.7322 2201.661 8163.5 127 3.364 28.9 1 K.LHLPSTSVLQRISLPLKQLG.Q Reverse_YER110C 2 2 4.3% 1113 122601 4.6 U KAP123 SGDID:S000000912, Chr V from 382099-378758, reverse complement, Verified ORF, "Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1" * Jamie_Ashy_SPB_73.26269.26269.3 2.341 0.0413 45.8 2697.5942 2701.046 8881.2 132 3.02 21.0 1 K.TGLALESLAASAVEKEYSIGTNVTFK.N * Jamie_Ashy_SPB_48.17191.17191.2 1.5002 0.155 16.4 2488.5723 2490.824 8709.0 9 3.426 26.2 1 P.AFNEGYVKALNAIFAYGSERLR.A Reverse_YOL100W 2 2 4.3% 1081 121660 9.6 U PKH2 SGDID:S000005460, Chr XV from 129236-132481, Verified ORF, "Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p" * Jamie_Ashy_SPB_52.18628.18628.4 3.2882 0.2114 59.1 3086.5764 3090.2903 5541.7 118 4.014 20.4 1 H.TNNNYLTSHTTEEVSPSSASRQTVLHSK.G * Jamie_Ashy_SPB_25.08702.08702.2 1.2858 0.019 60.9 1954.4722 1954.2666 8194.6 426 2.866 26.5 1 R.DSGSPMAQMSKANIKYPK.I YKR079C 2 2 4.3% 838 96816 5.9 U TRZ1 SGDID:S000001787, Chr XI from 588947-586431, reverse complement, Uncharacterized ORF, "tRNase Z, involved in RNA processing, has two putative nucleotide triphosphate-binding motifs (P-loop) and a conserved histidine motif, homolog of the human candidate prostate cancer susceptibility gene ELAC2" * Jamie_Ashy_SPB_85.30480.30480.2 1.3815 0.022 61.6 2358.632 2358.6157 8198.4 54 2.763 25.0 1 K.SFNVLKNGGEDRLGVFDSFQK.G * Jamie_Ashy_SPB_103.36945.36945.2 1.5368 0.0638 70.1 1834.2122 1832.9248 6115.0 76 3.1 39.3 1 K.ENSNQESNRKLELDR.D Q0050 2 2 4.3% 834 96079 9.9 U AI1 SGDID:S000007261, Chr Mito from 13818-16322, Verified ORF, "Reverse transcriptase required for splicing of the COX1 pre-mRNA, encoded by a mobile group II intron within the mitochondrial COX1 gene" * Jamie_Ashy_SPB_56.19923.19923.4 3.4311 0.078 16.4 4390.9766 4393.0566 6677.5 113 3.454 15.2 1 T.RVGRWTYEEPRTIINNYKALGRGILNYYKLATNYKR.L * Jamie_Ashy_SPB_115.41134.41134.2 1.0267 0.228 11.6 3501.0322 3503.044 3716.1 8 3.832 8.9 1 R.VGRWTYEEPRTIINNYKALGRGILNYYKL.A YDL148C 2 2 4.3% 810 94302 7.4 U NOP14 SGDID:S000002307, Chr IV from 190587-188155, reverse complement, Verified ORF, "Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA" * Jamie_Ashy_SPB_109.39043.39043.2 1.1596 0.0554 61.7 2351.152 2350.7288 6997.2 122 3.158 25.0 1 R.HIIFLSNQNYLKNVQSFKR.T * Jamie_Ashy_SPB_39.13964.13964.2 1.8625 0.0894 88.4 1894.0322 1891.2235 6493.1 10 3.694 40.0 1 K.LTKFTEHIPLALQNHK.P YFL054C 2 2 4.3% 646 70507 8.4 U YFL054C SGDID:S000001840, Chr VI from 22787-20847, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_12.04330.04330.3 1.7464 0.0073 33.2 1511.4543 1510.6932 6084.8 384 2.933 31.2 1 K.PSNTHIENVSRKK.K * Jamie_Ashy_SPB_25.08890.08890.3 2.2087 0.1395 14.3 1504.6444 1504.7697 4177.2 99 3.3 33.9 1 L.VIFGVGGNLQATVTK.G YBR163W 2 2 4.3% 585 67570 7.2 U DEM1 SGDID:S000000367, Chr II from 565718-567475, Verified ORF, "Protein of unknown function, shows similarity to RNA-processing protein Pta1p" * Jamie_Ashy_SPB_43.15306.15306.2 2.0824 0.1788 54.3 1551.3522 1549.8076 5612.4 338 3.801 46.2 1 N.DKSLDGTFILPSKK.N * Jamie_Ashy_SPB_103.37077.37077.2 1.7426 0.0472 13.7 1379.4521 1379.6854 4519.6 27 3.883 55.0 1 F.YTKWKTPLTLK.Y YEL022W 2 2 4.2% 1459 165667 5.1 U GEA2 SGDID:S000000748, Chr V from 111421-115800, Verified ORF, "Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs), involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; similar to but not functionally redundant with Gea1p" * Jamie_Ashy_SPB_13.04394.04394.4 3.599 0.1466 28.2 4352.0566 4352.9014 6258.3 1 3.196 22.1 1 L.RSIVDSPYGDLLSNSIIYDVLQTILSLACNNRRSEVLR.N * Jamie_Ashy_SPB_96.34570.34570.2 1.4579 0.1308 12.2 2584.8323 2585.8677 7793.0 23 3.781 25.0 1 S.KGLLSTFASYLKGDEEPTEEEIK.S Reverse_YDR407C 2 2 4.2% 1289 147662 7.5 U TRS120 SGDID:S000002815, Chr IV from 1287928-1284059, reverse complement, Verified ORF, "One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic" * Jamie_Ashy_SPB_15.05178.05178.2 2.1964 0.0403 14.0 1336.9321 1336.4471 4786.7 2 3.062 63.6 1 T.SNNSVFSTPQVR.K * Jamie_Ashy_SPB_112.40202.40202.4 1.5982 0.0751 35.1 4907.4565 4908.5625 4143.0 4 3.51 8.5 1 K.QLILWTSQSPDDVNIKAESENINLLEIITDILTRYDPHWAIK.N Reverse_YGR233C 2 2 4.2% 1178 134029 5.8 U PHO81 SGDID:S000003465, Chr VII from 958213-954677, reverse complement, Verified ORF, "Cyclin-dependent kinase (CDK) inhibitor, regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; required for derepression of PHO5; transcriptionally regulated by Pho4p and Pho2p" * Jamie_Ashy_SPB_25.08768.08768.2 2.0754 0.0292 13.0 1771.4521 1770.0391 6170.3 27 3.126 36.7 1 I.GNDQTLKISELGPRLK.L * Jamie_Ashy_SPB_78.27993.27993.4 3.5515 0.0836 13.8 4052.7766 4048.68 7312.4 1 3.293 19.8 1 K.SHLINFKIRLDSERALYYGNVKELERELKFFFA.A Reverse_YNL087W 2 2 4.2% 1178 132509 8.5 U TCB2 SGDID:S000005031, Chr XIV from 462413-465949, Verified ORF, "Bud-specific protein with a potential role in membrane trafficking; GFP-fusion protein migrates from the cell surface to intracellular vesicles near vacuole; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud via the mRNA transport system involving She2p" * Jamie_Ashy_SPB_44.15582.15582.2 1.7886 0.1517 28.1 1284.5721 1284.5852 4663.3 67 3.974 54.5 1 A.SKKGILNLVSPK.Y * Jamie_Ashy_SPB_102.36668.36668.4 2.8623 0.1311 16.8 4140.3364 4138.988 7457.7 54 3.838 17.1 1 N.WVPNASNKFVKTKGVIKGSLEFTLYPDVTKKIMGVLK.R Reverse_YAL019W 3 3 4.2% 1131 128507 5.4 U FUN30 SGDID:S000000017, Chr I from 114920-118315, Verified ORF, "Protein whose overexpression affects chromosome stability, potential Cdc28p substrate; homolog of Snf2p" * Jamie_Ashy_SPB_97.34705.34705.2 2.0748 0.2688 16.7 1981.4521 1979.9689 5479.8 6 4.58 36.1 1 D.EKEKSTADAGATNNNNSNN.N * Jamie_Ashy_SPB_7.02392.02392.3 2.201 7.0E-4 39.9 1524.3243 1527.6804 4668.1 281 2.811 34.6 1 K.SSKNNINGHISSLR.G * Jamie_Ashy_SPB_10.03297.03297.2 1.4804 0.0146 63.4 1607.8922 1608.8784 4567.8 494 3.292 35.7 1 K.KVPPPTESKKPSANK.N Reverse_YJR039W 2 2 4.2% 1121 127439 6.3 U YJR039W SGDID:S000003800, Chr X from 503850-507215, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_14.05084.05084.2 1.7919 0.041 12.7 1364.3922 1366.6005 6716.3 1 3.061 65.0 1 F.IVEKYLESTKR.K * Jamie_Ashy_SPB_7.02397.02397.4 1.7502 0.0303 33.0 4168.8564 4173.543 7870.9 25 3.006 11.4 1 R.AEDQEVGEFVDIYELGIVVLERSQGELGDEERIFCK.V Reverse_YMR196W 2 2 4.2% 1088 126624 5.4 U YMR196W SGDID:S000004809, Chr XIII from 655075-658341, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_13.04610.04610.3 2.2369 0.0846 57.7 2439.8643 2437.6873 6014.6 32 3.293 27.3 1 R.KMKPGQESSETSATTAVSSRPTR.P * Jamie_Ashy_SPB_45.16047.16047.3 2.2232 0.0052 37.4 2721.0244 2720.1465 7969.0 267 3.019 23.9 1 R.FFLQPAIYLEGSDIKSRNHITLR.F Reverse_YKL105C 3 3 4.2% 1132 125593 5.8 U YKL105C SGDID:S000001588, Chr XI from 242227-238829, reverse complement, Uncharacterized ORF, "Putative protein of unknown function" * Jamie_Ashy_SPB_4.01297.01297.3 1.6693 0.0108 33.2 1711.7344 1707.8406 4822.7 85 2.587 33.9 1 R.DHSKDKIAADDNHLK.H * Jamie_Ashy_SPB_26.09333.09333.2 2.1541 0.0244 11.6 1814.5521 1814.0488 4682.2 27 3.175 46.9 1 D.LNEAATATANKTILPER.L * Jamie_Ashy_SPB_105.37706.37706.2 1.964 0.1765 46.3 1705.1522 1705.8625 5586.8 4 4.119 36.7 1 K.LLDPAVRTTDTSSKSN.V Reverse_YPR097W 3 3 4.2% 1073 123054 5.8 U YPR097W SGDID:S000006301, Chr XVI from 725392-728613, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_39.13806.13806.3 2.5198 0.0452 58.9 2264.1843 2263.5933 6958.1 119 3.526 25.0 1 R.LKDIESEDVVLNLLDKYQK.E * Jamie_Ashy_SPB_6.02058.02058.2 1.5249 0.0905 13.4 1619.4521 1618.0592 6073.7 227 3.271 37.5 1 G.QKVKLDLLKKYIK.R * Jamie_Ashy_SPB_68.24352.24352.2 1.687 0.0919 14.2 1395.0922 1396.5834 4606.1 16 3.279 45.8 1 K.PNIDKIDLNPGSL.F Reverse_YNL238W 2 2 4.2% 814 90002 5.0 U KEX2 SGDID:S000005182, Chr XIV from 202429-204873, Verified ORF, "Subtilisin-like protease (proprotein convertase), a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway" * Jamie_Ashy_SPB_70.25209.25209.2 1.8433 0.0692 12.1 2159.9321 2161.4858 6748.9 106 3.72 25.0 1 K.VKIKWDGVGNEGWHAVSMF.T * Jamie_Ashy_SPB_60.21331.21331.2 1.8274 0.0767 14.2 1546.9321 1545.7794 5930.0 451 3.271 39.3 1 A.GKSDRGETVGKVLAK.K YNL262W 3 3 4.1% 2222 255669 7.0 U POL2 SGDID:S000005206, Chr XIV from 148213-154881, Verified ORF, "Catalytic subunit of DNA polymerase epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair" * Jamie_Ashy_SPB_46.16581.16581.4 2.8277 0.098 10.9 3187.9365 3192.7324 6317.7 85 3.452 20.1 1 R.ERLGSAIQKIITIPAALQGVSNPVPRVEHP.D * Jamie_Ashy_SPB_50.17942.17942.4 3.6365 0.0699 16.9 4337.4966 4336.82 9873.2 282 3.109 15.3 1 K.AVSKRKRNQLTNEEDPLVLPSEIPSMDEDYVGWLNYQ.K * Jamie_Ashy_SPB_52.18740.18740.2 2.261 0.041 15.7 2681.3123 2681.1504 8822.6 57 3.493 26.1 1 G.EPGVLEVFVTINGKVQNITFHIPK.T YKL182W 2 2 4.1% 2051 228689 5.9 U FAS1 SGDID:S000001665, Chr XI from 100676-106831, Verified ORF, "Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities" * Jamie_Ashy_SPB_52.18656.18656.3 2.6749 0.0888 75.6 3175.9443 3175.5193 7957.4 458 3.306 19.4 1 R.TTLDAEKVFTQGLNILEWLENPSNTPDK.D * Jamie_Ashy_SPB_85.30414.30414.4 2.2917 0.1576 15.2 5955.177 5950.833 8193.1 39 3.341 11.6 1 K.NKSGKIFVETKFSKLIGRPPLLVPGMTPCTVSPDFVAATTNAGYTIELAGGGYFSAA.G YFL033C 3 3 4.1% 1770 196530 6.5 U RIM15 SGDID:S000001861, Chr VI from 74425-69113, reverse complement, Verified ORF, "Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; originally identified as a regulator of IME2" * Jamie_Ashy_SPB_54.19484.19484.2 1.6365 0.0154 64.3 1653.8322 1653.0208 7863.1 13 2.785 42.3 1 R.KKLTGDYFAIKVLR.K * Jamie_Ashy_SPB_5.01617.01617.3 2.099 0.0731 54.9 1612.4343 1610.7703 3205.0 12 3.362 37.5 1 R.SHGSSNIDLPASLRR.S * Jamie_Ashy_SPB_106.38138.38138.4 2.2264 0.0485 43.9 4822.4966 4818.311 3904.8 491 3.304 12.4 1 K.PTISGSPSTFGTFPKTFLRSDSFSTRSYSPERSISIDSSTLSRK.G Reverse_YMR229C 2 2 4.1% 1729 193133 6.2 U RRP5 SGDID:S000004842, Chr XIII from 731122-725933, reverse complement, Verified ORF, "Protein required for the synthesis of both 18S and 5.8S rRNA; C-terminal region is crucial for the formation of 18S rRNA and N-terminal region is required for the 5.8S rRNA; component of small ribosomal subunit (SSU) processosome" * Jamie_Ashy_SPB_101.36276.36276.4 2.5231 0.2534 40.2 5114.5366 5114.853 9078.5 12 4.166 14.2 1 V.YEITKEDGESEEFQLWKNFFFKAQKRTIKKTIIREFLDEVK.K * Jamie_Ashy_SPB_119.42590.42590.4 2.8353 0.1048 15.6 3301.6965 3295.7136 7998.2 14 3.913 20.7 1 T.NDLKVFVGFDTVSKITGEFVDGAKIDSYTK.L YOR093C 2 2 4.1% 1648 186906 8.5 U YOR093C SGDID:S000005619, Chr XV from 502452-497506, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_60.21478.21478.3 2.6559 0.152 15.2 3297.0842 3298.9534 7522.1 395 3.583 20.2 1 V.VMKHNILINQFETMTKILNSRSMPHWK.Q * Jamie_Ashy_SPB_65.23445.23445.4 2.5994 0.1313 15.6 4764.0166 4765.3423 8722.0 28 3.161 13.8 1 R.YCSLELANSTVEKKFLNNDLSAQFVKFQFDNVILDFLPHSA.Y YNR031C 4 4 4.1% 1579 180527 5.9 U SSK2 SGDID:S000005314, Chr XIV from 685435-680696, reverse complement, Verified ORF, "MAP kinase kinase kinase of the HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress" * Jamie_Ashy_SPB_28.09817.09817.3 3.08 0.1744 23.9 2653.3145 2653.7795 7773.1 65 3.526 24.0 1 P.TRSQPNGSTSSSTLQDIVTTNSSQR.S * Jamie_Ashy_SPB_94.33699.33699.2 1.6098 0.0832 13.0 1842.4122 1842.1051 7421.6 159 3.349 30.0 1 D.RFQQISGSSVSFLEKK.S * Jamie_Ashy_SPB_8.02712.02712.2 1.6239 0.0377 74.9 872.89215 873.9877 3528.1 125 2.542 71.4 1 K.KIANNGTR.L * Jamie_Ashy_SPB_4.01431.01431.2 1.4373 0.0186 62.5 1840.1322 1842.2067 6349.7 406 2.919 30.0 1 R.ASAVELLMDPWIVQIR.E YIL002C 2 2 4.1% 946 108430 6.7 U INP51 SGDID:S000001264, Chr IX from 353428-350588, reverse complement, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth" * Jamie_Ashy_SPB_81.29164.29164.4 3.6183 0.1314 24.5 4222.7363 4220.8916 6326.7 56 3.803 16.2 1 L.LLFMNETEYSKVKHIEGDVKKTGFGGMASNKGAVAVSFK.Y * Jamie_Ashy_SPB_115.41268.41268.1 1.3637 0.1206 89.7 779.88 778.9267 2575.1 69 4.248 50.0 1 K.GAVAVSFK.Y Reverse_YOR335C 2 2 4.1% 958 107277 5.5 U ALA1 SGDID:S000005862, Chr XV from 949104-946228, reverse complement, Verified ORF, "Cytoplasmic alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog" * Jamie_Ashy_SPB_22.07940.07940.4 2.9782 0.0936 15.2 2798.9766 2803.1875 5681.6 227 3.063 21.5 1 K.KEKIPSFPLKDAADLDAAFQEALRQ.A * Jamie_Ashy_SPB_5.01636.01636.4 2.2609 0.1265 11.7 1685.4165 1683.942 3865.7 199 3.176 29.5 1 V.ETLLTWSYTIAEKK.F YPL249C 2 2 4.1% 894 101800 4.8 U GYP5 SGDID:S000006170, Chr XVI from 79353-76669, reverse complement, Verified ORF, "GTPase-activating protein (GAP) for yeast Rab family members, involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p" * Jamie_Ashy_SPB_16.05462.05462.3 2.2604 0.087 71.8 1499.5443 1496.7068 4613.7 77 3.329 32.7 1 R.QNVATSTSPKLPPR.G * Jamie_Ashy_SPB_60.21394.21394.3 2.7005 0.1491 13.7 2902.6143 2903.1436 5843.9 19 3.809 26.1 1 K.EDHYESLRIQNHQLQREAQKLEH.D Reverse_YJL200C 2 2 4.1% 789 86583 7.0 U YJL200C SGDID:S000003736, Chr X from 58813-56444, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_90.32410.32410.2 1.6993 0.0375 70.1 2172.7922 2175.322 8937.6 391 3.356 26.3 1 R.ASGEGYNHEAIVTWPRGDSK.W * Jamie_Ashy_SPB_56.20146.20146.2 1.5901 0.0831 12.5 1346.8322 1344.4302 4129.4 2 3.694 54.5 1 K.TEGDKGVILHDC.H YKR084C 2 2 4.1% 611 68730 5.4 U HBS1 SGDID:S000001792, Chr XI from 598532-596697, reverse complement, Verified ORF, "GTP binding protein with sequence similarity to the elongation factor class of G proteins, EF-1alpha and Sup35p; associates with Dom34p, and shares a similar genetic relationship with genes that encode ribosomal protein components" * Jamie_Ashy_SPB_61.21980.21980.2 2.0354 0.1471 28.8 2107.5923 2108.402 6801.7 24 4.957 29.4 1 K.GDFVTLKLRKAYPEDIQN.G * Jamie_Ashy_SPB_2.00598.00598.2 1.744 0.0067 10.2 869.3522 869.0579 3777.5 5 3.327 75.0 1 E.QPARIKR.L YDR261C 2 2 4.1% 562 63508 5.4 U EXG2 SGDID:S000002669, Chr IV from 979203-977515, reverse complement, Verified ORF, "Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor" * Jamie_Ashy_SPB_5.01663.01663.3 1.7159 0.049 39.1 1208.6344 1206.43 2777.2 162 3.189 41.7 1 R.ILYGDLGWLR.L * Jamie_Ashy_SPB_7.02492.02492.2 1.3879 0.0183 61.6 1571.0322 1572.8479 3928.9 80 2.693 45.8 1 R.LNNTKELTLAIWR.D YOR245C 2 2 4.1% 418 47711 9.6 U DGA1 SGDID:S000005771, Chr XV from 795332-794076, reverse complement, Verified ORF, "Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles" * Jamie_Ashy_SPB_6.02162.02162.2 1.8709 0.0631 20.5 813.0122 812.86066 2824.9 196 2.597 80.0 1 T.ERHENK.S * Jamie_Ashy_SPB_30.10804.10804.2 1.9376 0.0277 13.1 1231.3922 1231.4409 5754.8 2 2.991 60.0 1 G.ISSVSRKNALR.T Reverse_YER172C 5 5 4.0% 2163 246183 5.5 U BRR2 SGDID:S000000974, Chr V from 536016-529525, reverse complement, Verified ORF, "RNA-dependent ATPase RNA helicase involved in the facilitation and disruption of snRNA interactions, required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis" * Jamie_Ashy_SPB_9.03235.03235.3 2.2506 0.1105 14.9 1273.5844 1272.4501 2900.2 24 3.097 38.9 1 L.ELSQINKRQR.W * Jamie_Ashy_SPB_48.17064.17064.2 2.2879 0.0148 13.9 1967.8121 1968.3397 8446.5 345 3.715 36.7 1 S.KCLNLLMRTPHGWGRK.L * Jamie_Ashy_SPB_17.06052.06052.3 2.3647 0.0031 39.1 2487.4143 2486.8308 6496.0 131 3.022 23.8 1 K.LIQKSGADNKTLKHTINEEAFK.N * Jamie_Ashy_SPB_61.21848.21848.2 2.0892 0.0093 13.4 1623.8322 1624.8773 8084.0 10 3.692 42.3 1 R.FKYQEVLDNLGKAV.M * Jamie_Ashy_SPB_103.36910.36910.3 2.4439 0.1771 13.5 2901.9243 2905.9128 9272.3 274 3.543 20.7 1 K.NKKKSNVEVDNYDYEEDDDENDNI.E Reverse_YMR306W 2 2 4.0% 1785 207482 8.1 U FKS3 SGDID:S000004923, Chr XIII from 881158-886515, Verified ORF, "Protein of unknown function, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p" * Jamie_Ashy_SPB_76.27266.27266.4 2.4 0.074 59.8 5319.8564 5320.8354 7576.2 375 3.179 12.6 1 R.TRLTYSPESSNFGFYSFPLDNIKEKILDDETPLKRSETHDSLPSSR.P * Jamie_Ashy_SPB_78.27828.27828.3 3.3813 0.1433 18.8 3117.7744 3116.4143 7802.4 136 3.694 23.0 1 K.WKYDASKLNTQDELQEQTLTIKPHNN.I YGR116W 3 3 4.0% 1451 168291 5.0 U SPT6 SGDID:S000003348, Chr VII from 720413-724768, Verified ORF, "Essential protein that interacts with histones and is involved in nucleosome disassembly and reassembly during transcription elongation" * Jamie_Ashy_SPB_16.05779.05779.4 3.5266 0.084 14.5 2692.6565 2692.674 6430.1 12 3.648 28.3 1 E.ELENGNDNNEAEEEEIDEETGAIK.S * Jamie_Ashy_SPB_25.08812.08812.4 3.2917 0.1872 46.3 2574.8965 2578.882 3338.5 27 4.002 23.9 1 I.LNANSGDLQVFTSNTKLAIDTVQK.Y * Jamie_Ashy_SPB_55.19492.19492.2 1.763 0.2149 60.0 1147.5122 1147.3201 3950.0 1 4.184 72.2 1 T.LLKSNSSKNR.M YGL197W 3 3 4.0% 1487 167074 7.7 U MDS3 SGDID:S000003165, Chr VII from 124703-129166, Verified ORF, "Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions" * Jamie_Ashy_SPB_118.42355.42355.2 1.46 0.1134 13.3 1231.1122 1229.3348 7679.0 43 3.928 50.0 1 S.ATSQFENYIR.Y * Jamie_Ashy_SPB_88.31472.31472.4 3.0025 0.1335 13.4 3227.8164 3227.6292 7080.6 141 3.55 22.2 1 L.SRRRSSLMSIPSGELLRSSISEAEHQRR.A * Jamie_Ashy_SPB_31.11037.11037.3 2.6235 0.1192 16.1 2614.9143 2614.8777 7086.5 136 3.676 25.0 1 R.FRNKEDDKVTKDISNDKKRNY.L Reverse_YPL226W 2 2 4.0% 1196 134330 5.9 U NEW1 SGDID:S000006147, Chr XVI from 121767-125357, Verified ORF, "ATP binding cassette family member; Asn/Gln-rich rich region supports [NU+] prion formation, susceptibility to [PSI+] prion induction and aggregation of a fragment of the human Machado-Joseph Disease protein" * Jamie_Ashy_SPB_13.04621.04621.4 3.3546 0.01 68.2 3261.8965 3265.6226 6100.7 12 3.555 21.0 1 K.ALNSAKLGVADANGGDEFKSQDVQASGKQVMK.G * Jamie_Ashy_SPB_47.16696.16696.3 1.7569 0.0473 35.6 1745.8744 1741.972 4682.3 1 2.89 38.3 1 R.MLTSKGAGNRGCLGYR.H Reverse_YOL145C 2 2 4.0% 1077 124655 6.4 U CTR9 SGDID:S000005505, Chr XV from 52786-49553, reverse complement, Verified ORF, "Component of the Paf1p complex, which is a large complex that binds to and modulates the activity of RNA polymerase II and is required for expression of a subset of genes, including cyclin genes; contains TPR repeats" * Jamie_Ashy_SPB_118.42438.42438.4 3.1312 0.2073 45.5 2989.3765 2989.4941 9848.4 6 4.003 26.4 1 V.KQYLQIAKLYSHKSKGQEKPNRSKK.G * Jamie_Ashy_SPB_74.26518.26518.2 1.6731 0.077 12.5 2044.7922 2044.3976 7685.1 42 3.566 32.4 1 R.GCWFSSESLVIKAIMPSM.K YPR019W 2 2 4.0% 933 105003 6.2 U CDC54 SGDID:S000006223, Chr XVI from 596747-599548, Verified ORF, "Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt the DNA prior to replication; accumulates in the nucleus in G1; homolog of S. pombe Cdc21p" * Jamie_Ashy_SPB_65.23176.23176.4 2.9113 0.0143 55.9 2909.3364 2908.3105 4996.8 17 3.071 26.8 1 R.IIWGTNVSIQECTTNFRNFLMSFK.Y * Jamie_Ashy_SPB_13.04654.04654.2 1.9981 0.0061 10.9 1473.2722 1474.7618 4835.9 444 2.7 41.7 1 D.MNLVQTGKSVIQR.K YGL163C 2 2 4.0% 898 101754 9.0 U RAD54 SGDID:S000003131, Chr VII from 196409-193713, reverse complement, Verified ORF, "DNA-dependent ATPase, stimulates strand exchange by modifying the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family" * Jamie_Ashy_SPB_61.21987.21987.2 1.663 0.1124 15.0 1478.6721 1480.8119 6030.9 10 3.288 45.8 1 Y.VQRHSLPLTLGMK.K * Jamie_Ashy_SPB_32.11403.11403.2 1.5336 0.1064 11.8 2434.172 2436.9565 10485.5 2 3.59 29.5 1 K.KVVKGVGGSQPLRAIGILKKLCN.H YJL165C 2 2 4.0% 855 95455 8.4 U HAL5 SGDID:S000003701, Chr X from 109454-106887, reverse complement, Verified ORF, "Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters" * Jamie_Ashy_SPB_98.34998.34998.2 1.6663 0.131 13.2 2051.7922 2051.403 7406.3 6 3.431 34.4 1 D.LQNKKEEKARAKHVRSK.E * Jamie_Ashy_SPB_110.39493.39493.2 1.3384 0.2625 28.3 1994.6721 1992.2882 7506.9 1 4.924 31.2 1 N.GYHEHSLRVLPIITNNK.N YPL153C 2 2 4.0% 821 91962 7.8 U RAD53 SGDID:S000006074, Chr XVI from 264191-261726, reverse complement, Verified ORF, "Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p" * Jamie_Ashy_SPB_20.07166.07166.4 3.07 0.0697 18.0 2005.0565 2002.2096 4095.5 49 3.533 31.1 1 K.AQRKLQMEQQLQEQDQ.E * Jamie_Ashy_SPB_34.11953.11953.3 2.6577 0.1434 14.0 1851.4143 1849.0139 5384.6 1 3.531 40.6 1 H.SNNTENVKSSKKKGNGR.F Reverse_YER184C 2 2 4.0% 794 91077 7.8 U YER184C SGDID:S000000986, Chr V from 558675-556291, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_50.17947.17947.2 1.9182 0.1383 26.6 1731.5521 1733.0665 10013.0 3 3.524 39.3 1 F.HRNYLLGSFVATIIK.T * Jamie_Ashy_SPB_56.19923.19923.2 1.8024 0.1301 10.4 2195.9922 2196.291 6677.5 47 3.099 28.1 1 L.KRYEEATDEDQGIYYEW.R YLR305C 3 3 3.9% 1900 214605 7.5 U STT4 SGDID:S000004296, Chr XII from 743865-738163, reverse complement, Verified ORF, "Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization" * Jamie_Ashy_SPB_63.22484.22484.4 3.1539 0.1791 28.3 4102.5767 4106.5347 7144.4 60 3.592 17.7 1 N.VRSNKQFFTDMCKISLQFCSESHILDLSTDNRAR.F * Jamie_Ashy_SPB_9.03087.03087.2 1.8603 0.129 94.3 1564.2322 1561.9666 7505.9 254 3.848 42.3 1 K.IMFLAAAVLLETIR.C * Jamie_Ashy_SPB_52.18584.18584.3 2.9299 0.0987 15.7 2930.0645 2930.343 8243.7 5 2.983 24.0 1 E.EMSKIEVKPDVYLPSNPDGVVIDIDR.K Reverse_YJR137C 2 2 3.9% 1442 161218 5.5 U ECM17 SGDID:S000003898, Chr X from 683200-678872, reverse complement, Verified ORF, "Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine" * Jamie_Ashy_SPB_104.37432.37432.2 1.6666 0.0593 10.0 2035.6322 2033.2456 6249.9 392 3.555 29.4 1 S.SSSLRLGSFDTNDLKYTK.L * Jamie_Ashy_SPB_82.29274.29274.4 3.041 0.0957 17.2 4059.0566 4054.2825 6054.1 250 3.153 16.2 1 K.PEDDVAAGSVGLAEWLKPEWESYATQFGDADQDDGLGL.P Reverse_YFR016C 2 2 3.9% 1233 137698 4.5 U YFR016C SGDID:S000001912, Chr VI from 180735-177034, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_24.08366.08366.4 3.4501 0.1321 10.6 3463.8564 3466.7344 5812.2 82 3.745 17.8 1 E.GEQDEKAVELDEPIINKEVAEVIEVDDRPAD.P * Jamie_Ashy_SPB_17.06008.06008.4 2.6186 0.178 17.0 1859.7366 1861.926 3411.7 18 3.892 34.4 1 K.SLDTTDAPNSSNRGRRN.T Reverse_YBL007C 2 2 3.9% 1244 135848 5.9 U SLA1 SGDID:S000000103, Chr II from 216369-212635, reverse complement, Verified ORF, "Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; contains 3 SH3 domains; interacts with proteins regulating actin dynamics and with proteins required for endocytosis" * Jamie_Ashy_SPB_27.09730.09730.2 1.7625 0.0727 12.9 2116.8523 2118.135 9287.8 107 3.313 27.5 1 K.NSSNNNNSSEAPPVSTTNPVS.T * Jamie_Ashy_SPB_84.30193.30193.3 3.1185 0.0398 73.5 2911.8843 2914.3257 5188.7 16 3.529 25.0 1 K.KLVPAEEIYTSPVLGVPEESDSGIVRK.K contaminant_UBIQUITIN11 2 2 3.9% 1118 127523 8.5 U no description * Jamie_Ashy_SPB_106.37867.37867.2 1.979 0.2781 82.6 2117.2322 2115.4119 4704.7 10 4.839 32.4 1 L.LLFLMDGLHEDLNKADNR.K * Jamie_Ashy_SPB_81.28962.28962.3 2.7562 0.1561 13.8 2940.6243 2938.1511 7343.4 2 3.992 25.0 1 K.KYNLFSVSNHYGGLDGGHYTAYCKNA.A YGL156W 2 2 3.9% 1083 124499 7.3 U AMS1 SGDID:S000003124, Chr VII from 210421-213672, Verified ORF, "Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway" * Jamie_Ashy_SPB_14.04849.04849.4 2.8865 0.1601 11.5 2341.0964 2344.4565 6135.1 241 3.615 27.5 1 E.ECSLNGSYTSSVTGYDDYIVL.A * Jamie_Ashy_SPB_20.07231.07231.2 1.9736 0.0347 11.9 2378.2122 2376.6768 9579.4 54 3.07 27.5 1 K.YAIYPHRGALSSDTVKLAHEF.N YML100W 2 2 3.9% 1098 123018 6.6 U TSL1 SGDID:S000004566, Chr XIII from 70624-73920, Verified ORF, "Large subunit of trehalose 6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex, which converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose, homologous to Tps3p and may share function" * Jamie_Ashy_SPB_31.11205.11205.4 3.3612 0.0889 16.5 3273.2966 3278.6128 7165.3 67 3.417 20.7 1 L.WPTLHYQIPDNPNSKAFEDHSWKFYR.N * Jamie_Ashy_SPB_50.17695.17695.2 1.8901 0.0851 15.1 2021.1921 2023.3336 6869.6 24 3.071 34.4 1 K.FLVENPEYVEKSTLIQI.C YBL076C 2 2 3.9% 1072 122983 6.1 U ILS1 SGDID:S000000172, Chr II from 84259-81041, reverse complement, Verified ORF, "Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A" * Jamie_Ashy_SPB_56.19999.19999.4 3.0827 0.1517 15.6 3272.3364 3266.6062 6409.1 8 3.448 21.4 1 R.GFKVMPYSTGLTTPLSNFEAQQNYKDVND.P * Jamie_Ashy_SPB_27.09432.09432.2 2.0993 0.1063 27.4 1543.2322 1542.9059 5356.2 54 3.289 45.8 1 I.REKKTISLKTPLK.T YJL050W 2 2 3.9% 1073 122055 6.3 U MTR4 SGDID:S000003586, Chr X from 342438-345659, Verified ORF, "Dead-box family ATP dependent helicase required for mRNA export from the nucleus; co-factor of the exosome complex, required for 3' end formation of 5.8S rRNA" * Jamie_Ashy_SPB_114.40869.40869.1 1.3224 0.1649 16.7 899.02 899.0752 5330.7 229 4.048 50.0 1 K.VVVPVLADS.F * Jamie_Ashy_SPB_37.13292.13292.3 2.4396 0.0841 66.8 3783.7744 3783.4092 8418.7 109 3.299 16.4 1 K.TVVAEYAIAQSLKNKQRVIYTSPIKALSNQKYR.E Reverse_YBR038W 2 2 3.9% 963 109882 8.6 U CHS2 SGDID:S000000242, Chr II from 311897-314788, Verified ORF, "Chitin synthase II, requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for the synthesis of chitin in the primary septum during cytokinesis" * Jamie_Ashy_SPB_16.05781.05781.3 3.0596 0.0904 15.9 2920.5244 2923.299 7439.5 235 4.06 21.9 1 K.NEEKLCFVLQVPVIDKSVYDLDANI.S * Jamie_Ashy_SPB_119.42564.42564.3 2.3197 0.0553 64.2 1512.3243 1511.8259 7129.5 60 2.82 39.6 1 K.KKSPPPLDSVMRR.K Reverse_YIL002C 2 2 3.9% 946 108430 6.7 U INP51 SGDID:S000001264, Chr IX from 353428-350588, reverse complement, Verified ORF, "Phosphatidylinositol 4,5-bisphosphate 5-phosphatase, synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth" * Jamie_Ashy_SPB_114.40771.40771.4 3.0664 0.0907 16.9 2951.4165 2954.2659 3886.5 39 3.471 22.2 1 R.GRKPEPIPTPSHTPGAISDSGEFSEIVF.A * Jamie_Ashy_SPB_13.04404.04404.2 1.8232 0.0112 10.8 914.9522 914.1332 5319.6 122 2.948 62.5 1 K.IINVISVVG.Y YFL023W 2 2 3.9% 796 91422 4.7 U BUD27 SGDID:S000001871, Chr VI from 90984-93374, Verified ORF, "Protein involved in bud-site selection, nutrient signaling, and gene expression controlled by the TOR kinase; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern" * Jamie_Ashy_SPB_2.00567.00567.2 1.8796 0.0242 81.7 926.8122 927.0452 3200.3 45 3.316 62.5 1 K.EQTPAVGPK.E * Jamie_Ashy_SPB_11.03942.03942.4 2.2658 0.1475 11.6 2534.9365 2537.7488 4289.6 254 3.489 26.2 1 R.AQKQSQDHEREEGDVNKSLKPI.L Reverse_YLR206W 2 2 3.9% 613 71807 5.5 U ENT2 SGDID:S000004196, Chr XII from 554580-556421, Verified ORF, "Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus" * Jamie_Ashy_SPB_14.05042.05042.1 1.4457 0.1193 92.4 1334.64 1335.4166 3538.7 362 3.747 36.4 1 K.DSISQNGTRTQK.L * Jamie_Ashy_SPB_6.01869.01869.1 1.0887 0.1662 88.2 1476.75 1477.7217 2664.0 28 3.318 22.7 1 R.RPRPRAARNARR.N Reverse_YHR165C 5 5 3.8% 2413 279502 7.3 U PRP8 SGDID:S000001208, Chr VIII from 436950-429709, reverse complement, Verified ORF, "Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing" * Jamie_Ashy_SPB_66.23580.23580.3 3.3613 0.1563 20.7 3144.1143 3144.551 6548.9 68 3.956 21.2 1 V.DWIESLNNIGQTWKYVLLPPIESDSPK.I * Jamie_Ashy_SPB_45.16116.16116.2 1.652 0.1397 17.0 1487.0922 1486.6273 5073.5 13 3.714 40.9 1 K.KIRNLTDHPNDY.A * Jamie_Ashy_SPB_49.17626.17626.2 1.6714 0.0727 12.6 2183.112 2183.478 6500.5 344 3.612 27.8 1 R.SELWEVMVSFITTAEEPTI.E * Jamie_Ashy_SPB_53.19006.19006.2 1.6992 0.083 12.8 2552.8523 2553.124 9957.4 14 3.664 28.6 1 K.SKIYREIIKKIPAPMGPVDWPI.N * Jamie_Ashy_SPB_31.10968.10968.2 1.7659 0.0558 13.0 1297.4122 1296.5125 5195.0 19 3.515 55.0 1 A.ESLHQLITRAK.K YBR275C 5 5 3.8% 1916 217959 6.6 U RIF1 SGDID:S000000479, Chr II from 757101-751351, reverse complement, Verified ORF, "Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation" * Jamie_Ashy_SPB_7.02299.02299.2 2.165 0.0519 18.0 1129.4521 1129.2584 3676.3 132 3.131 66.7 1 I.QSDLSPTPKR.R * Jamie_Ashy_SPB_68.24513.24513.2 1.911 0.0926 14.4 1773.5721 1771.1124 7473.4 1 4.083 40.0 1 S.LPGNPELFSGLLPFLR.R * Jamie_Ashy_SPB_8.02868.02868.4 2.615 0.1316 16.0 2195.9365 2193.7625 3469.4 2 3.791 32.4 1 E.LLEKIIIQKEIQILEKLK.E * Jamie_Ashy_SPB_39.13999.13999.2 1.947 0.0919 17.9 1835.5122 1835.9318 7960.1 206 3.332 33.3 1 R.NSIKVETNCNENSLNV.T * Jamie_Ashy_SPB_36.12654.12654.2 1.8992 0.0806 16.0 1533.8121 1530.8545 4430.4 433 3.496 37.5 1 Q.GSKSQIKEKLKKR.L YLR310C 2 2 3.8% 1589 179091 7.1 U CDC25 SGDID:S000004301, Chr XII from 756995-752226, reverse complement, Verified ORF, "Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1" * Jamie_Ashy_SPB_57.20277.20277.4 3.0104 0.0152 59.1 3086.4966 3083.6047 6211.4 71 2.718 21.2 1 K.KKSSQIFEKLNNATGEHLKIISKPKSR.I * Jamie_Ashy_SPB_26.09255.09255.4 3.7488 0.1053 16.6 3722.3364 3720.1252 8664.2 17 3.303 18.8 1 K.IPGAEDLLQKINEKLINENEKEPVDPKQQDSVS.A Reverse_contaminant_IGA1_HAEIN 2 2 3.8% 1541 169369 6.9 U owl|P42782| IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE).... Reverse_contaminant_IGA3_HAEIN 2 2 3.8% 1545 170626 6.9 U owl|P45385| IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE).... Jamie_Ashy_SPB_80.28664.28664.4 2.6045 0.0559 61.4 4624.2964 4628.9834 9000.5 120 3.243 15.1 1 K.ARADSLVANTNTSTLDCLAVTSRDNSSTTAPEVNHPVSRVSRR.H Jamie_Ashy_SPB_25.08772.08772.2 2.2428 0.1952 67.2 1461.6721 1461.6122 9704.6 8 4.213 50.0 1 K.LSGKNDGTGEVILTG.K YGL131C 3 3 3.8% 1403 163203 8.6 U SNT2 SGDID:S000003099, Chr VII from 265864-261653, reverse complement, Verified ORF, "DNA binding protein with similarity to the S. pombe Snt2 protein" * Jamie_Ashy_SPB_11.03866.03866.2 1.8358 0.1061 19.9 1479.2922 1480.763 5607.3 201 3.544 55.0 1 A.FKKCKRELSRK.F * Jamie_Ashy_SPB_85.30642.30642.2 1.4644 0.0313 64.5 2041.3322 2042.5227 5851.7 219 2.855 26.5 1 R.GLTVRGKLSKLSKNKRKK.E * Jamie_Ashy_SPB_79.28161.28161.4 2.5541 0.0805 69.3 2907.2166 2910.1394 6258.9 11 2.888 23.6 1 K.TSDTSRNRKCNDVFIEKASNNNIPK.I YOR296W 3 3 3.8% 1289 147426 8.6 U YOR296W SGDID:S000005822, Chr XV from 870199-874068, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_20.06978.06978.2 1.5293 0.0298 63.2 1493.9722 1493.7434 4893.2 422 3.182 34.6 1 R.TINNLLTAIFSGTK.P * Jamie_Ashy_SPB_41.14564.14564.2 1.3207 0.0142 61.2 2343.4321 2340.772 8898.4 13 2.515 30.0 1 R.NITLQREIGTTKIVARSITPK.W * Jamie_Ashy_SPB_117.41827.41827.2 1.548 0.1156 12.0 1781.2122 1781.0154 8221.3 194 3.257 34.6 1 K.DEVQRLIPLYEEYL.R YNL091W 2 2 3.8% 1240 141514 5.4 U NST1 SGDID:S000005035, Chr XIV from 452410-456132, Verified ORF, "Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1" * Jamie_Ashy_SPB_1.00371.00371.2 2.6593 0.028 29.6 1416.7522 1415.6359 6960.9 5 3.211 70.0 1 Q.QLAKEEEKRKR.E * Jamie_Ashy_SPB_52.18538.18538.4 2.871 0.1711 11.6 3669.8164 3675.021 7437.3 2 3.518 20.5 1 S.SFSGPSTAGNFNNSSIQSGMLLAPTLGSVESFPNRT.S YPL226W 2 2 3.8% 1196 134331 5.9 U NEW1 SGDID:S000006147, Chr XVI from 121767-125357, Verified ORF, "ATP binding cassette family member; Asn/Gln-rich rich region supports [NU+] prion formation, susceptibility to [PSI+] prion induction and aggregation of a fragment of the human Machado-Joseph Disease protein" * Jamie_Ashy_SPB_57.20322.20322.2 1.9239 0.2227 63.5 2084.5522 2086.4417 8146.9 1 4.135 35.3 1 E.NKKLAYYKGNLAAFVEQK.P * Jamie_Ashy_SPB_54.19266.19266.4 3.4278 0.1606 95.6 3114.4966 3116.5188 5650.4 3 4.048 25.3 1 K.SYYTLTDSNAQMRFPPPGILTGVKSNTR.A YER033C 2 2 3.8% 1076 119350 4.9 U ZRG8 SGDID:S000000835, Chr V from 221286-218056, reverse complement, Verified ORF, "Cytoplasmic protein of unknown function, transcription is induced under conditions of zinc deficiency" * Jamie_Ashy_SPB_7.02466.02466.1 2.8054 9.0E-4 27.3 847.53 846.9157 5541.5 16 2.742 83.3 1 S.QNETLNK.K * Jamie_Ashy_SPB_58.20650.20650.4 3.0319 0.1136 16.0 4042.8564 4045.3955 7070.2 78 3.143 18.7 1 K.NKIELTNKTVTFFNSNNWNTYDCNSLSRKTSSQM.R Reverse_YMR231W 2 2 3.8% 1029 117477 5.8 U PEP5 SGDID:S000004844, Chr XIII from 733544-736633, Verified ORF, "Peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; forms complex with Pep3p that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, also interacts with Pep7p" * Jamie_Ashy_SPB_29.10192.10192.2 1.9376 0.1289 23.5 1886.7122 1888.1301 8454.2 7 3.457 40.0 1 R.RDLDVEFTSPKFKGTF.V * Jamie_Ashy_SPB_84.30003.30003.3 2.2777 0.0248 42.1 2771.0645 2775.1797 5569.0 2 3.085 26.1 1 K.KFLYDGYKKHIEQVDLPSLSHQK.A YML076C 2 2 3.8% 944 107560 7.6 U WAR1 SGDID:S000004541, Chr XIII from 115347-112513, reverse complement, Verified ORF, "Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively" * Jamie_Ashy_SPB_57.20308.20308.2 1.465 0.0114 62.3 2080.5923 2080.3635 7127.8 258 2.94 27.8 1 R.SRSPTPFESPMVNVSTKSK.G * Jamie_Ashy_SPB_76.27246.27246.2 1.4797 0.0299 64.2 1978.2922 1978.2589 7159.7 55 2.946 34.4 1 K.HRPKIVGDEETQNVKVK.R Reverse_Q0050 2 2 3.8% 834 96079 9.9 U AI1 SGDID:S000007261, Chr Mito from 13818-16322, Verified ORF, "Reverse transcriptase required for splicing of the COX1 pre-mRNA, encoded by a mobile group II intron within the mitochondrial COX1 gene" * Jamie_Ashy_SPB_45.16236.16236.2 1.8139 0.0769 83.8 1901.4922 1900.2646 7747.3 450 3.31 26.7 1 K.RNMTIMRGTIYDKTAK.L * Jamie_Ashy_SPB_10.03408.03408.3 2.5482 0.1376 87.7 1964.5743 1961.275 5639.2 2 4.041 43.3 1 K.LEKIILDHSITDFCKK.L YIL038C 2 2 3.8% 836 94403 5.6 U NOT3 SGDID:S000001300, Chr IX from 282651-280141, reverse complement, Verified ORF, "Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation" * Jamie_Ashy_SPB_35.12456.12456.2 1.699 0.0709 12.5 1595.8121 1598.7502 6419.0 43 3.172 38.5 1 R.LLANEELDPQDVKN.V * Jamie_Ashy_SPB_29.10422.10422.2 2.2116 0.0571 17.8 1976.1322 1977.0056 4738.3 49 3.11 35.3 1 R.DVSVDNNNVNDQSNVTLE.Q YNL212W 2 2 3.8% 782 88846 5.1 U VID27 SGDID:S000005156, Chr XIV from 247462-249810, Verified ORF, "Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth" * Jamie_Ashy_SPB_58.20884.20884.2 1.7161 0.0701 12.5 1471.5922 1471.3417 6675.0 4 3.036 45.8 1 R.DKESSESENDSED.E * Jamie_Ashy_SPB_89.31742.31742.3 2.2272 0.0655 55.6 1992.1743 1994.2268 4744.3 24 3.375 32.8 1 K.MDIERGKVIEEWSTGDK.N YBR260C 2 2 3.8% 666 74619 8.3 U RGD1 SGDID:S000000464, Chr II from 734634-732634, reverse complement, Verified ORF, "GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization" * Jamie_Ashy_SPB_10.03474.03474.2 1.8378 0.0308 11.8 1362.5721 1361.5647 5768.0 6 3.414 61.1 1 L.TCEEFMKFIR.K * Jamie_Ashy_SPB_43.15282.15282.2 1.474 0.0705 69.3 1613.5721 1614.7123 6841.3 150 2.964 39.3 1 K.DVSDAIHSAEKAQSR.Y Reverse_YJL127C 2 2 3.8% 640 72922 6.2 U SPT10 SGDID:S000003663, Chr X from 184139-182217, reverse complement, Verified ORF, "Putative histone acetylase, required for transcriptional regulation at core promoters, functions at or near the TATA box" * Jamie_Ashy_SPB_7.02450.02450.2 1.584 0.0788 79.7 1332.4922 1332.4247 3255.9 35 3.05 50.0 1 K.ENRDCHLPYK.G * Jamie_Ashy_SPB_12.04286.04286.4 2.5055 0.1311 16.5 1800.1765 1806.078 4855.2 125 3.574 32.1 1 R.REKRKKYQTTHKTT.E YEL065W 2 2 3.8% 628 70562 8.1 U SIT1 SGDID:S000000791, Chr V from 27657-29543, Verified ORF, "Ferrioxamine B transporter, member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p" * Jamie_Ashy_SPB_5.01759.01759.2 1.6213 0.0711 14.1 948.0122 947.146 4371.4 402 3.106 58.3 1 L.HMRYLAR.K * Jamie_Ashy_SPB_45.16194.16194.2 1.6675 0.1083 17.7 2138.0923 2139.4648 5320.0 110 3.126 34.4 1 E.IKYSRHPLTPWDLIQDR.G Reverse_YLR227W-B 3 3 3.7% 1755 198404 7.9 U YLR227W-B SGDID:S000007376, Chr XII from 593440-594744,594746-598708, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Ashy_SPB_39.13760.13760.2 1.8984 0.026 12.4 1501.8722 1502.7598 8941.3 111 3.832 41.7 1 A.GSQKLGYHSKKLR.I Jamie_Ashy_SPB_103.36914.36914.4 2.7041 0.0197 50.8 4362.4165 4358.0405 7782.9 86 2.985 17.1 1 K.HYAEIYKEKEKIDKNYTIAEDYRLTTRIPKISKVAK.A Jamie_Ashy_SPB_55.19845.19845.2 1.9789 0.1236 23.7 1742.2522 1741.9419 8750.0 3 4.215 40.0 1 G.GLSSNTQRSNIPPLEK.F Reverse_YHR186C 2 2 3.7% 1557 177608 7.8 U KOG1 SGDID:S000001229, Chr VIII from 480672-475999, reverse complement, Verified ORF, "Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors" * Jamie_Ashy_SPB_81.29128.29128.2 1.3975 0.1265 12.7 2753.4521 2755.059 8500.2 125 3.093 22.7 1 I.REDECKYINVISDHSNTAAIMMR.H * Jamie_Ashy_SPB_96.34308.34308.4 2.9425 0.1011 16.6 3790.0566 3788.2515 7509.1 25 3.416 18.2 1 K.IQKTGTAIIPAHEHVQMCTMTTPKQQSGYQSTVN.Q Reverse_YPR010C 3 3 3.7% 1203 135742 7.7 U RPA135 SGDID:S000006214, Chr XVI from 581193-577582, reverse complement, Verified ORF, "RNA polymerase I subunit A135" * Jamie_Ashy_SPB_4.01392.01392.2 1.72 0.0717 12.0 1584.4122 1586.7506 5836.8 52 3.331 46.2 1 Q.GHRSSFKDGIQPTR.R * Jamie_Ashy_SPB_7.02402.02402.2 1.8787 0.0716 15.5 1321.2522 1321.5192 5553.8 86 3.411 50.0 1 F.KDGLYQLVQTR.N * Jamie_Ashy_SPB_66.23564.23564.3 2.4995 0.1133 11.0 2294.6343 2292.6028 3652.6 6 3.655 29.2 1 P.FASKDKPPNIFRSERELTR.F YDL223C 2 2 3.7% 1046 113616 6.4 U HBT1 SGDID:S000002382, Chr IV from 60406-57266, reverse complement, Verified ORF, "Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis" * Jamie_Ashy_SPB_48.17180.17180.4 3.1373 0.1498 11.0 4528.5366 4525.418 8040.9 442 3.893 14.0 1 N.SSKGSDYDYNNSTHSAEHTPRHHQYGSDEGEQDYHDDEQ.G * Jamie_Ashy_SPB_7.02310.02310.2 1.9316 0.0343 17.5 1036.7722 1036.0922 6567.2 7 3.723 68.8 1 S.THSAEHTPR.H YLR373C 2 2 3.7% 901 102499 5.2 U VID22 SGDID:S000004365, Chr XII from 871366-868661, reverse complement, Verified ORF, "Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles" * Jamie_Ashy_SPB_52.18619.18619.2 2.0607 0.0293 13.0 1975.0322 1972.928 8389.5 218 3.073 28.9 1 V.SSATAATTATNTDTDTDGDR.D * Jamie_Ashy_SPB_40.14190.14190.2 1.8546 0.028 10.8 1549.5521 1549.7179 5811.0 391 2.873 45.8 1 R.PIIEEYQSSIQIE.K YLR277C 3 3 3.7% 779 87674 5.2 U YSH1 SGDID:S000004267, Chr XII from 699497-697158, reverse complement, Verified ORF, "Putative endonuclease, subunit of the mRNA cleavage and polyadenylation specificity complex required for 3' processing of mRNAs" * Jamie_Ashy_SPB_2.00573.00573.2 1.9298 0.0409 15.9 1098.4722 1097.1295 4905.6 2 3.089 75.0 1 T.GDYSREVDR.H * Jamie_Ashy_SPB_4.01140.01140.2 1.7236 0.074 15.6 951.0122 951.1106 3918.0 2 3.065 78.6 1 D.FLSLSDLR.E * Jamie_Ashy_SPB_5.01843.01843.3 2.4892 0.1059 18.9 1441.2244 1438.6267 5167.9 391 3.702 36.4 1 H.HPDLSTTILRER.Q YKL203C 3 3 3.6% 2474 281566 7.5 U TOR2 SGDID:S000001686, Chr XI from 63360-55936, reverse complement, Verified ORF, "PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis" * Jamie_Ashy_SPB_26.09391.09391.2 1.6002 0.088 80.3 1495.6522 1496.8302 5499.9 4 2.986 54.2 1 K.PYIDPILDVILPK.C * Jamie_Ashy_SPB_57.20281.20281.4 2.6921 0.1306 18.8 5605.8965 5603.2676 5141.6 152 3.079 13.1 1 E.FLEEPKNSTIEALISINNQLHQTDSAIGILKHAQQHNELQLKETWYEK.L * Jamie_Ashy_SPB_73.26226.26226.4 3.0772 0.0984 18.0 3291.1765 3294.8362 6238.1 235 3.934 21.0 1 F.RLTRMLTYAMEVSGIEGSFRITCENVMK.V YDL140C 3 3 3.6% 1733 191610 5.6 U RPO21 SGDID:S000002299, Chr IV from 210562-205361, reverse complement, Verified ORF, "RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime" * Jamie_Ashy_SPB_74.26619.26619.4 3.0986 0.1119 14.6 2759.1765 2757.2368 5308.2 391 3.862 25.8 1 I.MDNDPVLFNRQPSLHKMSMMAHR.V * Jamie_Ashy_SPB_88.31634.31634.3 1.8969 0.0522 38.7 2376.8044 2373.73 7183.9 44 3.007 28.9 1 K.KIENTMLENITLRGVENIER.V * Jamie_Ashy_SPB_24.08611.08611.2 1.8859 0.1205 10.5 1975.0521 1976.1034 7804.3 5 3.588 36.1 1 G.FSPTSPTYSPTSPAYSPTS.P YOL004W 2 2 3.6% 1536 174838 5.6 U SIN3 SGDID:S000005364, Chr XV from 316938-321548, Verified ORF, "DNA binding subunit of Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression of meiosis-specific genes during vegetative growth and silencing" * Jamie_Ashy_SPB_61.21920.21920.4 3.1528 0.0718 11.5 4712.2563 4706.4624 9143.6 52 3.24 14.6 1 I.MKDFKSQAIDTPGVIERVSTLFRGYPILIQGFNTFLPQGYR.I * Jamie_Ashy_SPB_40.14326.14326.2 2.0221 0.0741 16.6 2028.3522 2029.2692 7401.8 488 3.425 28.6 1 K.RLKQKDEEWRRAQRE.W YDR127W 2 2 3.6% 1588 174754 6.3 U ARO1 SGDID:S000002534, Chr IV from 704479-709245, Verified ORF, "Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids" * Jamie_Ashy_SPB_46.16442.16442.2 1.6179 0.0953 13.3 2803.672 2803.3254 9329.1 89 3.468 21.2 1 C.TRDTVMVAIGGGVIGDMIGFVASTFMR.G * Jamie_Ashy_SPB_27.09529.09529.4 3.1428 0.0894 19.7 3176.7766 3177.7678 6711.5 4 3.315 21.8 1 Q.QKVVIPPGSKSISNRALILAALGEGQCKIK.N Reverse_YJL095W 3 3 3.6% 1478 164195 9.5 U BCK1 SGDID:S000003631, Chr X from 247171-251607, Verified ORF, "Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p" * Jamie_Ashy_SPB_65.23425.23425.4 2.8413 0.099 12.0 3853.0166 3851.3882 6343.6 203 3.36 17.7 1 R.NVKQKMKLSPSTSTSDQDTKLRRMVDNAGKYPQP.I * Jamie_Ashy_SPB_64.22815.22815.2 1.3424 0.1148 10.4 1222.1721 1220.4723 4063.2 342 3.231 45.0 1 K.PSTKFVMKPAN.E * Jamie_Ashy_SPB_3.00776.00776.2 1.8709 0.0312 14.3 1040.1322 1039.2194 5515.4 43 2.498 71.4 1 K.KLPYFKQD.L YJR033C 2 2 3.6% 1357 154932 8.2 U RAV1 SGDID:S000003794, Chr X from 496365-492292, reverse complement, Verified ORF, "Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate" * Jamie_Ashy_SPB_61.21744.21744.4 3.2153 0.0733 15.6 4043.2566 4048.5388 7563.4 150 3.379 16.2 1 R.GIFEVKSDSLDIDGDDIYKISIIDPVHQTFVSNRPL.I * Jamie_Ashy_SPB_79.28336.28336.2 1.6107 0.0833 12.2 1498.7522 1496.6837 4872.0 4 3.041 50.0 1 A.HTAHNIGDSVWMK.N Reverse_YOL138C 2 2 3.6% 1341 149269 7.0 U YOL138C SGDID:S000005498, Chr XV from 65349-61324, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_57.20464.20464.2 1.6908 0.0613 13.3 1632.3922 1633.981 7207.3 5 3.572 41.7 1 S.KRHERKQLIPISK.A * Jamie_Ashy_SPB_72.25801.25801.4 2.8019 0.1342 16.2 3979.0166 3984.1084 6696.9 143 3.46 17.2 1 K.AIPSAHFQASKTPSDHPQEEEQHEHEDEEIIVDDS.V YGL092W 2 2 3.6% 1317 145661 5.7 U NUP145 SGDID:S000003060, Chr VII from 337909-341862, Verified ORF, "Essential nucleoporin, catalyzes its own cleavage in vivo to generate a C-terminal fragment that assembles into the Nup84p subcomplex of the nuclear pore complex, and an N-terminal fragment of unknown function that is homologous to Nup100p" * Jamie_Ashy_SPB_83.29719.29719.3 2.0924 0.0019 33.8 3396.1743 3398.8442 9759.6 12 2.717 19.4 1 R.RTYSFSSSVSGNAPLPLASQSSLVSRLSTRLK.A * Jamie_Ashy_SPB_4.01450.01450.2 1.8417 0.1335 22.5 1688.4122 1686.0513 4550.7 2 3.297 50.0 1 K.LKIDSNRSAAKKLKL.L YPL012W 2 2 3.6% 1228 137508 7.0 U RRP12 SGDID:S000005933, Chr XVI from 529720-533406, Verified ORF, "Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; contains HEAT-repeats" * Jamie_Ashy_SPB_95.34214.34214.4 2.8129 0.043 65.4 3545.2166 3542.931 5449.1 153 3.168 18.7 1 K.PSTNVDDPLSAVTSGINAYLEAVKSGPVRGQRNK.L * Jamie_Ashy_SPB_7.02211.02211.3 1.6768 0.0068 33.3 1208.3944 1204.5045 3626.1 18 2.897 44.4 1 K.KGPKFKSRKK.L YMR196W 2 2 3.6% 1088 126624 5.4 U YMR196W SGDID:S000004809, Chr XIII from 655075-658341, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_63.22488.22488.2 1.7448 0.0527 10.9 2067.412 2068.2969 7783.0 296 3.342 27.8 1 R.LKERLFGLTGPQGNHGEDV.K * Jamie_Ashy_SPB_56.20077.20077.2 2.4121 0.1411 98.8 2022.7122 2022.1332 7220.4 7 3.915 39.5 1 R.TPRSSVATTASTESSEQGPK.M Reverse_YJL084C 2 2 3.6% 1046 117216 6.9 U YJL084C SGDID:S000003620, Chr X from 277918-274778, reverse complement, Verified ORF, "Cytoplasmic protein of unknown function that interacts with Pcl7p, phosphorylated in vitro; potential Cdc28p substrate" * Jamie_Ashy_SPB_59.20990.20990.2 1.7999 0.045 75.3 2292.5122 2289.545 6384.4 349 3.253 22.5 1 -.LTSENISQLGSENDLTNILVK.E * Jamie_Ashy_SPB_98.35038.35038.3 2.3014 0.2072 11.1 2049.5344 2051.183 3964.2 97 3.632 31.2 1 S.TNTSHRNLENKDHNKLE.D YHL007C 2 2 3.6% 939 102362 7.3 U STE20 SGDID:S000000999, Chr VIII from 97933-95114, reverse complement, Verified ORF, "Signal transducing kinase of the PAK (p21-activated kinase) family, involved in pheromone response and pseudohyphal/invasive growth pathways, activated by Cdc42p; binds Ste4p at a GBB motif present in noncatalytic domains of PAK kinases" * Jamie_Ashy_SPB_46.16556.16556.2 1.6114 0.0888 12.3 2424.632 2426.6946 9899.9 33 3.626 26.3 1 M.KDVFTSFVQNIKRNSQDDKR.A * Jamie_Ashy_SPB_23.08197.08197.2 1.8416 0.1215 10.3 1428.3722 1429.6188 5305.1 5 3.3 50.0 1 S.SSANGKFIPSRPAP.K Reverse_YGL027C 2 2 3.6% 833 96507 5.1 U CWH41 SGDID:S000002995, Chr VII from 446148-443647, reverse complement, Verified ORF, "Processing alpha glucosidase I, ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress" * Jamie_Ashy_SPB_50.17733.17733.1 1.5399 0.1542 25.0 1471.57 1471.6097 5035.4 7 4.17 41.7 1 K.GFYDDQRSLSLLG.Y * Jamie_Ashy_SPB_69.24664.24664.2 1.7535 0.1572 23.5 1928.6721 1928.1516 6844.1 99 4.09 28.1 1 F.FGRSPVSTFLEFPGEEK.A Reverse_YMR078C 2 2 3.6% 741 84373 8.3 U CTF18 SGDID:S000004683, Chr XIII from 424727-422502, reverse complement, Verified ORF, "Subunit of a complex with Ctf8p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint" * Jamie_Ashy_SPB_40.14265.14265.2 1.8733 0.0695 15.7 1086.0122 1088.2493 5613.5 150 3.969 55.6 1 M.ILSVIDSNAR.K * Jamie_Ashy_SPB_64.23062.23062.2 1.8834 0.0538 81.2 1976.3722 1976.3268 6949.7 3 3.305 37.5 1 R.KKDKKDPQGYLLKNTAK.I Reverse_YLR399C 2 2 3.6% 686 76978 6.0 U BDF1 SGDID:S000004391, Chr XII from 921596-919536, reverse complement, Verified ORF, "Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p" * Jamie_Ashy_SPB_9.03025.03025.2 2.0167 0.0901 17.2 1778.5922 1777.9321 4354.5 2 3.737 41.2 1 R.KRSGASTPSLSSADRTVG.S * Jamie_Ashy_SPB_8.02713.02713.2 1.995 0.0218 16.2 876.0722 875.077 5929.7 89 3.261 75.0 1 F.KMAQQLR.K YNL172W 3 3 3.5% 1748 196143 6.0 U APC1 SGDID:S000005116, Chr XIV from 310638-315884, Verified ORF, "Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition" * Jamie_Ashy_SPB_11.03800.03800.2 1.9158 0.0408 13.2 1545.3322 1546.7332 5723.5 242 3.379 41.7 1 A.DLQRCSAIINSIR.S * Jamie_Ashy_SPB_75.26846.26846.2 1.4826 0.1676 16.2 3025.4922 3022.5986 7147.2 4 4.202 20.4 1 K.LIKVISVHLVAFLPSGSSDLNIDLKLQT.A * Jamie_Ashy_SPB_97.34917.34917.2 1.2647 0.0372 61.8 2544.9922 2546.9583 8695.9 73 3.289 25.0 1 R.EWASNMILWEFIGDDLSFIMK.D Reverse_YER190W 4 4 3.5% 1681 190502 7.4 U YRF1-2 SGDID:S000000992, Chr V from 571475-576520, Verified ORF, "Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p" Reverse_YPL283C 4 4 3.1% 1859 211113 7.2 U YRF1-7 SGDID:S000006204, Chr XVI from 6007-5989,5840-280, reverse complement, Verified ORF, "Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p" Reverse_YNL339C 4 4 3.1% 1859 211097 7.2 U YRF1-6 SGDID:S000005283, Chr XIV from 6098-6080,5931-371, reverse complement, Verified ORF, "Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p" Reverse_YGR296W 4 4 3.1% 1859 211113 7.2 U YRF1-3 SGDID:S000003528, Chr VII from 1084870-1084888,1085037-1090597, Verified ORF, "Helicase encoded by the Y' element of subtelomeric regions, highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p" Jamie_Ashy_SPB_45.16218.16218.2 1.7455 0.0794 11.8 1671.3322 1673.9764 7704.0 27 3.697 38.5 1 K.NCFLGILMSLFATW.H Jamie_Ashy_SPB_113.40635.40635.4 2.4387 0.0236 43.7 4224.5767 4228.85 6040.4 2 3.228 19.0 1 R.LQHLDRFEFSSGFLKQGAVLIDNTSKPERPRKRTVR.S Jamie_Ashy_SPB_63.22536.22536.3 2.6733 0.0474 60.6 2825.8743 2825.1968 7305.7 12 3.097 25.0 1 R.FEFSSGFLKQGAVLIDNTSKPERPR.K Jamie_Ashy_SPB_4.01161.01161.2 1.667 0.0579 82.2 1047.2922 1046.2144 5763.7 471 2.706 57.1 1 K.PERFLVER.N YLR247C 2 2 3.5% 1556 180343 6.5 U YLR247C SGDID:S000004237, Chr XII from 633356-628686, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_55.19579.19579.3 1.9302 0.0118 33.6 2384.6042 2381.6482 7733.8 130 2.962 23.7 1 R.RKLKDSEATFIDDENRTITK.T * Jamie_Ashy_SPB_37.13358.13358.3 2.2766 0.047 45.2 3574.1343 3574.1155 7298.9 386 3.269 15.2 1 R.ISDSLVSLHSLSAPQLSHLIRTINKSLGGTLDAK.I YDR406W 2 2 3.5% 1529 172255 7.9 U PDR15 SGDID:S000002814, Chr IV from 1279200-1283789, Verified ORF, "ATP binding cassette (ABC) transporter of the plasma membrane; general stress response factor implicated in cellular detoxification; target of Pdr1p, Pdr3p and Pdr8p transcription regulators; promoter contains a PDR responsive element" * Jamie_Ashy_SPB_91.32660.32660.4 2.2137 0.1635 15.1 2664.6565 2668.0393 4907.3 193 3.207 25.4 1 K.EFIEKGTRVPQTPKDMAEYWLQ.S * Jamie_Ashy_SPB_95.34143.34143.4 3.1716 0.0974 16.9 3115.5764 3113.2268 6325.4 2 3.237 22.8 1 S.PDSATTEKKILDDSSEGSDSSSDNAGLGLSK.S YDR443C 2 2 3.5% 1420 160000 5.8 U SSN2 SGDID:S000002851, Chr IV from 1349928-1345666, reverse complement, Verified ORF, "Protein required for stable association of Srb10p-Srb11p kinase with RNA polymerase holoenzyme; subunit of the RNA polymerase II mediator complex; essential for transcriptional regulation" * Jamie_Ashy_SPB_33.11815.11815.4 3.1695 0.1712 21.5 4300.0166 4297.809 5934.8 69 3.51 15.8 1 S.NKEGFNSIWKIPQNDIPQTESPLKTVDSSIQPIESNIK.M * Jamie_Ashy_SPB_86.30774.30774.2 1.8434 0.1061 91.1 1500.0322 1499.6294 5789.6 3 3.246 45.5 1 K.FKKCDGDTVWDK.F YGR061C 2 2 3.5% 1358 148904 5.3 U ADE6 SGDID:S000003293, Chr VII from 615969-611893, reverse complement, Verified ORF, "Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway" * Jamie_Ashy_SPB_13.04458.04458.2 2.1182 0.0459 16.4 2033.8121 2032.2253 4619.8 66 3.03 40.0 1 L.YHEGVRSQFSKFFNER.Q * Jamie_Ashy_SPB_40.14443.14443.4 2.9904 0.1681 93.6 3303.2966 3308.8035 8027.7 43 3.612 20.0 1 R.FPFNPNGSTNGIAGIKSPNGRVLAMMPHPER.V Reverse_YMR109W 3 3 3.5% 1219 136899 9.3 U MYO5 SGDID:S000004715, Chr XIII from 486586-490245, Verified ORF, "One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization" * Jamie_Ashy_SPB_93.33382.33382.3 2.4091 0.126 11.1 2187.0842 2191.4087 5214.9 78 3.902 27.6 1 I.VNASTNDHQVSNLVPKPVDR.N * Jamie_Ashy_SPB_16.05763.05763.2 1.9513 0.1092 22.0 1440.1721 1440.7008 5777.6 203 3.587 50.0 1 H.TSSAAAIYQMIRK.A * Jamie_Ashy_SPB_8.02678.02678.3 1.9252 0.0132 38.6 1220.2144 1221.4482 3440.7 2 3.224 47.2 1 R.KAPEQHVKKR.A Reverse_contaminant_UBIQUITIN02 2 2 3.5% 1058 117790 5.9 U no description * Jamie_Ashy_SPB_59.21031.21031.2 1.5982 0.0935 12.2 3019.4922 3022.247 9766.7 280 3.492 20.0 1 R.RDMYMRADVNDLANAVGDLNQFFDDD.Y * Jamie_Ashy_SPB_26.09376.09376.2 1.5578 0.1157 17.1 1287.8722 1287.4094 4918.1 466 3.223 50.0 1 K.DETLVEKDQPL.C YAL035W 2 2 3.5% 1002 112268 5.8 U FUN12 SGDID:S000000033, Chr I from 76428-79436, Verified ORF, "GTPase, required for general translation initiation by promoting Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; homolog of bacterial IF2" * Jamie_Ashy_SPB_36.12769.12769.2 1.8389 0.0904 15.3 1773.7522 1772.8347 7172.8 44 2.752 35.3 1 R.EASASAEGAEAIEGDFMS.T * Jamie_Ashy_SPB_16.05518.05518.2 1.6134 0.0823 13.0 1955.6122 1955.2769 6217.9 408 3.623 28.1 1 L.VIDIMHGLEQQTIESIK.L YNL126W 2 2 3.5% 846 98227 7.5 U SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * Jamie_Ashy_SPB_42.14856.14856.2 1.4535 0.0192 63.3 2162.892 2165.6477 8038.0 6 2.977 32.4 1 K.FHYRDVVFALKNILLMGK.S * Jamie_Ashy_SPB_32.11564.11564.2 3.0628 0.292 100.0 1381.4122 1380.4943 5785.3 1 5.769 81.8 1 K.NDGDEELFLLSK.S YHR172W 2 2 3.5% 823 96825 6.4 U SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * Jamie_Ashy_SPB_32.11547.11547.2 2.9246 0.2164 100.0 1681.9922 1680.9165 7038.0 1 4.714 53.8 1 K.NMNAILFDELSKER.T * Jamie_Ashy_SPB_17.05978.05978.2 2.5549 0.1541 99.6 1652.3121 1651.8162 6038.7 12 4.142 42.9 1 R.TGAYHGSNLELYTPK.S YOR270C 2 2 3.5% 840 95529 5.5 U VPH1 SGDID:S000005796, Chr XV from 830571-828049, reverse complement, Verified ORF, "Subunit of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes" * Jamie_Ashy_SPB_53.18864.18864.2 1.7714 0.053 12.6 2178.5522 2177.4363 6277.1 284 3.399 25.0 1 I.NMFLSPGTIDDELYPHQAK.V * Jamie_Ashy_SPB_52.18573.18573.2 1.2868 0.0287 61.6 1283.2522 1283.5619 4220.1 27 2.657 50.0 1 K.PLHFKFTHKK.K YDL056W 2 2 3.5% 833 93908 9.3 U MBP1 SGDID:S000002214, Chr IV from 352877-355378, Verified ORF, "Transcription factor involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes" * Jamie_Ashy_SPB_30.10543.10543.4 2.9191 0.0147 55.6 2694.4565 2690.1333 5952.7 381 2.724 22.9 1 K.ILGNPTAAPRKRGRPVGSTRGSRRK.L * Jamie_Ashy_SPB_15.05301.05301.2 1.6398 0.1568 17.9 1800.2922 1798.0614 4564.4 495 3.733 31.2 1 R.GRPVGSTRGSRRKLGVN.L YLL054C 2 2 3.5% 769 89163 7.8 U YLL054C SGDID:S000003977, Chr XII from 35203-32894, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_13.04669.04669.2 2.1884 0.1007 25.8 1785.9521 1786.9948 8011.6 168 3.746 29.4 1 P.ASMSASGSFTSILNPSTK.D * Jamie_Ashy_SPB_16.05588.05588.2 1.6683 0.0857 16.6 1049.2522 1049.3165 4469.9 61 3.447 56.2 1 K.LLALMNVFQ.Y YDR324C 2 2 3.5% 776 87801 6.6 U UTP4 SGDID:S000002732, Chr IV from 1116758-1114428, reverse complement, Verified ORF, "Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA" * Jamie_Ashy_SPB_68.24284.24284.4 3.1949 0.1457 10.6 2758.2166 2754.0325 4297.2 105 3.426 25.0 1 D.KLSVGCDNGTVVLIDISGGPGVLEHDT.I * Jamie_Ashy_SPB_35.12462.12462.2 2.0344 0.2024 63.3 1932.5521 1931.1162 7601.5 21 4.497 31.6 1 K.LSVGCDNGTVVLIDISGGPG.V Reverse_YML082W 2 2 3.5% 649 74313 5.3 U YML082W SGDID:S000004547, Chr XIII from 101862-103811, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_38.13519.13519.4 2.642 0.0971 16.1 1771.4965 1767.1246 2965.7 85 3.329 33.3 1 K.KFLKNEGVHPKLIKD.L * Jamie_Ashy_SPB_6.02038.02038.1 1.5442 0.171 16.7 834.98 834.815 4771.3 31 4.279 57.1 1 R.GADEEVSQ.E YLR361C 2 2 3.5% 578 66463 5.9 U DCR2 SGDID:S000004353, Chr XII from 849123-847387, reverse complement, Verified ORF, "Putative phosphoesterase that functions as a dosage-dependent positive regulator of the G1/S phase transition by controlling the timing of START" * Jamie_Ashy_SPB_6.01915.01915.2 1.8132 0.1859 49.1 1122.0521 1124.3405 4812.6 85 4.257 55.6 1 G.MYKEGVTAPK.Y * Jamie_Ashy_SPB_3.00792.00792.2 1.8967 0.0967 17.9 1165.6522 1165.2974 4965.6 9 3.717 61.1 1 A.GYGGTERRIR.I Reverse_YBR275C 3 3 3.4% 1916 217959 6.6 U RIF1 SGDID:S000000479, Chr II from 757101-751351, reverse complement, Verified ORF, "Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation" * Jamie_Ashy_SPB_55.19543.19543.2 1.9216 0.1078 16.7 2075.9321 2076.2363 6424.6 122 3.827 30.6 1 K.NRSNIAGVQHKEQAVTGHE.D * Jamie_Ashy_SPB_67.23871.23871.2 1.8443 0.1213 19.2 2295.612 2293.363 8727.9 25 3.332 31.6 1 R.ISENDVEELTEDDISRSRNA.H * Jamie_Ashy_SPB_56.20089.20089.3 2.4656 0.1741 14.8 2767.1343 2763.0793 9227.3 4 3.691 24.0 1 K.SSPSPKPSSGPIRYIPSSEIRDSFAV.S Reverse_YOR326W 2 2 3.4% 1574 180679 6.3 U MYO2 SGDID:S000005853, Chr XV from 925716-930440, Verified ORF, "One of two type V myosins, involved in polarized distribution of mitochondria; required for mitochondrion and vacuole inheritance and nuclear spindle orientation; moves multiple cargo" * Jamie_Ashy_SPB_9.03054.03054.2 1.5882 0.0941 12.8 1495.1721 1496.7994 6606.7 239 3.435 37.5 1 R.QAARRRILSQVVV.T * Jamie_Ashy_SPB_69.24772.24772.4 2.6851 0.0199 49.5 4672.2563 4666.341 7333.1 70 3.064 15.4 1 R.TIIQKKTVWKAFNYADIGLLECALKLNPEDASLSADNRTKK.I Reverse_YGL150C 2 2 3.4% 1489 171455 5.7 U INO80 SGDID:S000003118, Chr VII from 225578-221109, reverse complement, Verified ORF, "ATPase that forms a large complex, containing actin and several actin-related proteins, that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; shows similarity to the Snf2p family of ATPases" * Jamie_Ashy_SPB_47.16748.16748.4 3.1469 0.1531 15.6 2910.9766 2909.3872 5864.5 53 3.563 24.2 1 Y.STVMVHFPANKNYRLNKRDWFKR.L * Jamie_Ashy_SPB_75.26792.26792.3 2.2609 0.0103 38.2 3198.6243 3202.418 10625.9 13 2.727 21.3 1 R.LQEDNENDFDIAHFDNKNPALASIDINK.Q YDR180W 2 2 3.4% 1493 171101 6.8 U SCC2 SGDID:S000002588, Chr IV from 821290-825771, Verified ORF, "Putative protein kinase and subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX" * Jamie_Ashy_SPB_48.17011.17011.3 2.9009 0.1787 16.7 3367.8843 3364.0325 6749.4 68 4.171 18.8 1 D.SMLRYIALLSTIVLFNIFLLGKNDSNLHR.E * Jamie_Ashy_SPB_97.34869.34869.3 2.9383 0.1513 92.4 2786.3643 2783.1558 6335.1 308 3.94 21.4 1 K.QEEFLFNIVEHINDTILERFFK.N Reverse_YAL029C 2 2 3.4% 1471 169343 7.6 U MYO4 SGDID:S000000027, Chr I from 92271-87856, reverse complement, Verified ORF, "One of two type V myosins; required for mother-specific HO expression, for the bud tip localization of ASH1 and IST2 mRNA; facilitates growth and orientation of ER tubules along with She3p" * Jamie_Ashy_SPB_82.29533.29533.4 3.0829 0.1293 12.0 3849.5366 3851.4805 6741.0 154 3.559 18.2 1 K.ESNPKICRIYHVNTSNIIAMLEGLSKKFMSGLTP.K * Jamie_Ashy_SPB_91.32689.32689.2 1.788 0.083 14.8 1991.3722 1990.2688 6301.2 49 3.456 33.3 1 V.PEPLGELIQYFIHYNR.E Reverse_YCR042C 2 2 3.4% 1407 161470 5.7 U TAF2 SGDID:S000000638, Chr III from 205392-201169, reverse complement, Verified ORF, "TFIID subunit (150 kDa), involved in RNA polymerase II transcription initiation" * Jamie_Ashy_SPB_35.12520.12520.2 2.0632 0.0165 78.3 3427.412 3427.869 5390.7 2 3.463 24.1 1 R.ECVHQFHSSTLSNNPLDGSMAQLFIKPLVR.S * Jamie_Ashy_SPB_2.00640.00640.4 3.3124 0.1626 40.0 2169.4165 2166.5286 4393.6 5 3.9 33.3 1 K.CDFTVYELNQILPIIIIT.A Reverse_YOR124C 2 2 3.4% 1272 146354 5.2 U UBP2 SGDID:S000005650, Chr XV from 558642-554824, reverse complement, Verified ORF, "Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins, cleaves at the C terminus of ubiquitin fusions; capable of cleaving polyubiquitin and possesses isopeptidase activity" * Jamie_Ashy_SPB_98.35218.35218.3 2.0872 0.2175 14.8 3594.9243 3592.941 7014.3 59 4.172 18.3 1 R.NAIYTLADFYLGINEPDLSSLSEYNRIIDRD.T * Jamie_Ashy_SPB_31.11101.11101.2 1.8693 0.0288 13.4 1543.4521 1541.7748 5979.5 5 2.834 54.5 1 S.ENKVCMANYRKK.I Reverse_YPR022C 2 2 3.4% 1133 130117 7.1 U YPR022C SGDID:S000006226, Chr XVI from 607309-603908, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_59.21285.21285.3 2.7301 0.1807 10.3 2357.6943 2360.6292 7500.1 2 4.193 30.0 1 P.RTIVPDSNINYSKVVTPVNNE.K * Jamie_Ashy_SPB_50.17785.17785.2 1.9342 0.0543 11.6 2056.8523 2057.2368 6660.3 6 3.55 38.2 1 P.TSEIGGNNVKNRRINNNR.L Reverse_YOR188W 2 2 3.4% 1137 129971 8.9 U MSB1 SGDID:S000005714, Chr XV from 685767-689180, Verified ORF, "Protein involved in positive regulation of both 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway, potential Cdc28p substrate; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4" * Jamie_Ashy_SPB_32.11401.11401.3 2.5985 0.1557 14.0 2450.0942 2450.751 6858.6 395 3.743 25.0 1 G.TEIVPLQYEDPNLTSFQSLVR.E * Jamie_Ashy_SPB_6.02121.02121.2 1.3304 0.152 12.7 2087.9521 2085.262 5304.2 19 3.937 26.5 1 D.DMSNHENKTRGNSRPVNK.E Reverse_YNL054W 2 4 3.4% 1165 128140 6.0 U VAC7 SGDID:S000004999, Chr XIV from 526087-529584, Verified ORF, "Integral vacuolar membrane protein involved in vacuole inheritance and morphology; may function to regulate Fab1p kinase activity" * Jamie_Ashy_SPB_35.12546.12546.4 2.9301 0.1472 90.5 2611.8965 2609.2837 4478.2 439 3.364 22.7 1 R.ENTNNEDDGDDGDDDDDDDNEHK.A * Jamie_Ashy_SPB_21.07566.07566.2 2.8497 0.0085 17.5 1815.3322 1816.1082 8245.0 109 4.341 40.6 3 H.PSVKSLSVLSSRKDPSK.A YMR231W 2 2 3.4% 1029 117477 5.8 U PEP5 SGDID:S000004844, Chr XIII from 733544-736633, Verified ORF, "Peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; forms complex with Pep3p that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, also interacts with Pep7p" * Jamie_Ashy_SPB_16.05545.05545.2 1.6927 0.0838 14.8 883.15216 884.1099 2827.5 218 3.236 64.3 1 S.IGKPSLIR.V * Jamie_Ashy_SPB_15.05210.05210.4 3.7672 0.0632 15.5 2981.6565 2985.4941 4775.1 58 3.74 23.1 1 G.VPTTSISVNELSPTIINRIFIIDAKNK.I Reverse_YJR061W 2 2 3.4% 935 108427 6.6 U YJR061W SGDID:S000003822, Chr X from 550425-553232, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_5.01633.01633.3 2.2138 0.0024 42.5 1338.7444 1339.4478 5636.7 80 2.834 43.2 1 K.QDSGFINNSTKK.N * Jamie_Ashy_SPB_60.21552.21552.2 1.9537 0.0618 16.4 2163.9321 2165.5151 7735.1 144 3.459 28.9 1 R.NPVFASVGSFMTNRLPSIIN.L YPR105C 2 2 3.4% 861 98665 6.1 U COG4 SGDID:S000006309, Chr XVI from 739567-736982, reverse complement, Verified ORF, "Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments" * Jamie_Ashy_SPB_16.05532.05532.2 1.6082 0.0759 12.1 913.9322 913.9597 2599.6 24 4.029 64.3 1 K.AIHDDIET.V * Jamie_Ashy_SPB_84.30021.30021.2 1.566 0.0073 63.6 2153.7122 2156.314 6614.4 56 3.111 27.5 1 R.NLLGTVFVNSASSNSSTSNQK.N YER162C 2 2 3.4% 754 87204 7.8 U RAD4 SGDID:S000000964, Chr V from 502889-500625, reverse complement, Verified ORF, "Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); homolog of human XPC protein" * Jamie_Ashy_SPB_59.21166.21166.2 1.7189 0.16 27.1 1402.8922 1402.5564 4057.4 8 3.863 50.0 1 I.KPSSKRNSDARR.T * Jamie_Ashy_SPB_55.19689.19689.2 1.4547 0.1009 10.7 1565.6322 1567.915 5615.8 12 3.201 42.3 1 K.PVLSGIVVAKWLRE.A contaminant_KERATIN17 3 3 3.4% 590 62461 8.1 U no description * Jamie_Ashy_SPB_39.14050.14050.3 2.7603 0.155 90.9 2529.2043 2529.784 7415.2 11 4.207 30.3 1 R.NMQDLVEDFKNKYEDEINKR.T Jamie_Ashy_SPB_12.04278.04278.3 2.709 0.1939 98.8 1309.1943 1309.4215 4923.1 113 4.503 47.2 1 K.NKYEDEINKR.T Jamie_Ashy_SPB_5.01832.01832.2 2.1983 0.1982 81.2 1194.6921 1195.3176 4523.2 20 4.61 75.0 1 N.KYEDEINKR.T YLR454W 4 4 3.3% 2628 303478 8.0 U YLR454W SGDID:S000004446, Chr XII from 1043995-1051881, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * Jamie_Ashy_SPB_69.24656.24656.2 1.859 0.1183 10.5 1682.0521 1683.0067 6626.2 1 4.665 42.3 1 C.PKNKILKENSHVKF.L * Jamie_Ashy_SPB_1.00370.00370.4 2.6441 0.0302 51.8 3392.2966 3396.9417 6110.7 235 2.893 19.4 1 R.GTSVVSLMSIFLAVSGIHTLNQIFGHCIHQK.M * Jamie_Ashy_SPB_81.29083.29083.3 2.6434 0.1167 11.9 3173.3342 3172.7966 6358.2 13 3.527 22.1 1 I.NKKVLPFSTNPFLMNLIIENIDIDIIR.P * Jamie_Ashy_SPB_15.05295.05295.2 2.0615 0.1102 21.5 1838.0521 1838.0477 5428.6 3 3.411 39.3 1 F.INYMYHNADADQILR.K Reverse_YBL004W 4 4 3.3% 2493 287559 7.0 U UTP20 SGDID:S000000100, Chr II from 227639-235120, Uncharacterized ORF, "Possible snoRNA-binding protein, based on computational analysis of large-scale protein-protein interaction data" * Jamie_Ashy_SPB_105.37738.37738.2 1.382 0.0138 61.4 1330.1122 1329.595 5109.3 278 3.0 50.0 1 K.IINLVKRACNK.F * Jamie_Ashy_SPB_29.10423.10423.2 2.0275 0.042 12.1 1661.2922 1661.911 7225.5 79 2.772 42.9 1 K.LMSISKSAENLNPKE.N * Jamie_Ashy_SPB_103.36897.36897.3 2.5061 0.1402 15.7 2127.9844 2124.487 4377.5 58 3.398 32.8 1 F.HLFTFLLPLWELESYSK.Y * Jamie_Ashy_SPB_3.01120.01120.4 1.9611 0.2449 12.6 4406.7764 4406.2144 4252.4 129 4.266 8.3 1 R.VTLLGFLYKLFFSSVLKDTKTKGFEAGVNKIRITLDRAN.Q Reverse_YDR420W 3 3 3.3% 1802 188947 4.8 U HKR1 SGDID:S000002828, Chr IV from 1306257-1311665, Verified ORF, "Serine/threonine rich cell surface protein that contains an EF hand motif; involved in the regulation of cell wall beta-1,3 glucan synthesis and bud site selection; overexpression confers resistance to Hansenula mrakii killer toxin, HM-1" * Jamie_Ashy_SPB_77.27704.27704.4 2.9581 0.2138 43.4 2725.8164 2719.8726 3880.8 2 4.046 28.3 1 R.KYLEEDLADLEEIHVDSVSGNDDI.D * Jamie_Ashy_SPB_118.42456.42456.4 2.6488 0.1374 15.4 2183.4565 2186.3428 5836.2 2 4.085 28.3 1 K.SSDVFNHSLGTVSNHSSPISL.S * Jamie_Ashy_SPB_69.24649.24649.2 1.8822 0.1415 25.1 1689.9122 1688.9188 8048.8 395 4.049 35.7 1 E.KSIQSDTTIIPSIER.P Reverse_YLR320W 2 2 3.3% 1454 167682 6.2 U MMS22 SGDID:S000004312, Chr XII from 771940-776304, Verified ORF, "Protein involved in resistance to ionizing radiation; acts with Mms1p in a repair pathway that may be involved in resolving replication intermediates or preventing the damage caused by blocked replication forks" * Jamie_Ashy_SPB_43.15234.15234.4 2.9293 0.1162 16.9 4644.3765 4645.5254 8299.9 3 3.885 17.1 1 E.QFRKELNLDFRTALVILLNLRNKLVSLSDNMSIDGMPIVR.Q * Jamie_Ashy_SPB_42.15158.15158.4 3.2602 0.0828 18.2 2698.1365 2696.0168 5446.4 47 3.207 25.0 1 K.LVSLSDNMSIDGMPIVRQTDSSSLE.C YER008C 3 3 3.3% 1336 154694 5.4 U SEC3 SGDID:S000000810, Chr V from 171817-167807, reverse complement, Verified ORF, "Non-essential subunit of the exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p, Sec15p, Exo70p, Exo84p) which mediates targeting of post-Golgi vesicles to sites of active exocytosis; Sec3p specifically is a spatial landmark for secretion" * Jamie_Ashy_SPB_33.11875.11875.2 1.3335 0.0309 62.4 1140.0521 1140.2009 7814.4 100 3.454 50.0 1 R.TISGSSAHHSR.N * Jamie_Ashy_SPB_38.13470.13470.3 2.5433 0.0019 48.2 2074.1042 2074.3599 6003.6 23 2.834 36.7 1 R.LQLQKENEMKRLEEER.R * Jamie_Ashy_SPB_20.06987.06987.3 2.5808 0.0253 55.8 2266.1943 2269.4973 5516.5 152 3.261 31.2 1 R.KRQMELEHQRQLEEEER.K Reverse_YEL025C 2 2 3.3% 1188 136069 8.1 U YEL025C SGDID:S000000751, Chr V from 106147-102581, reverse complement, Uncharacterized ORF, "SWI/SNF and RSC interacting protein 1" * Jamie_Ashy_SPB_85.30450.30450.3 2.9098 0.1411 13.0 3078.8943 3079.6665 8179.4 492 3.549 20.2 1 S.ALCDNKPERLKKPIIKTAVQTISGLRK.F * Jamie_Ashy_SPB_59.21096.21096.2 1.8193 0.1514 28.3 1440.6921 1441.5804 5374.6 131 4.106 40.9 1 C.EPYESPYVISTR.I YPL217C 2 2 3.3% 1183 135571 6.8 U BMS1 SGDID:S000006138, Chr XVI from 143170-139619, reverse complement, Verified ORF, "Essential conserved nucleolar GTP-binding protein required for synthesis of 40S ribosomal subunits and for processing of the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, has similarity to Tsr1p" * Jamie_Ashy_SPB_119.42721.42721.4 1.901 0.0464 36.9 3450.8564 3454.8389 8406.8 160 3.204 18.5 1 R.NEHPYMLADRFTDLTHPELIETQGLQIDR.K * Jamie_Ashy_SPB_3.00780.00780.2 1.6977 0.0915 15.9 1124.5322 1123.0752 4377.1 73 3.519 55.6 1 P.SEQAEDNSDK.E YIL128W 2 2 3.3% 1032 117882 5.9 U MET18 SGDID:S000001390, Chr IX from 113806-116904, Verified ORF, "DNA repair and TFIIH regulator, required for both nucleotide excision repair (NER) and RNA polymerase II (RNAP II) transcription; possible role in assembly of a multiprotein complex(es) required for NER and RNAP II transcription" * Jamie_Ashy_SPB_25.08913.08913.2 2.1995 0.0557 15.6 2320.4722 2321.806 8689.5 110 3.775 27.5 1 K.VISSTFPLFLINTSEVAKLKL.L * Jamie_Ashy_SPB_13.04605.04605.2 1.9701 0.0931 17.4 1501.8922 1501.816 4191.8 138 3.701 45.8 1 A.TKLNRVAKISKSR.E Reverse_YNL014W 2 2 3.3% 1044 115868 6.2 U HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells" * Jamie_Ashy_SPB_93.33477.33477.2 1.8945 0.0283 10.6 2091.612 2093.3826 5897.2 133 3.107 28.9 1 N.DVTETAKTITTTAAEKVGKK.T * Jamie_Ashy_SPB_24.08415.08415.2 1.8308 0.0391 11.0 1524.0322 1522.6525 5460.1 465 2.686 38.5 1 S.SIGERTESTATELK.R YLL040C 3 3 3.2% 3144 357850 5.6 U VPS13 SGDID:S000003963, Chr XII from 63644-54210, reverse complement, Verified ORF, "Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; homologous to human COH1; involved in sporulation, vacuolar protein sorting and protein-Golgi retention" * Jamie_Ashy_SPB_17.05913.05913.3 2.8756 0.1147 12.4 1684.5844 1686.0537 4651.0 9 3.551 40.4 1 I.WSGDVKLKNLKLRK.D * Jamie_Ashy_SPB_107.38232.38232.4 1.5359 0.0552 33.5 6460.1367 6458.4766 6495.8 32 3.141 7.9 1 R.MGIESLLEDHKTVNVSLDLQAPLIILPLDPHDWDTPCAIIDAGHMSILSDLVPKEKIK.E * Jamie_Ashy_SPB_111.39688.39688.3 1.3126 0.0304 33.5 3413.0044 3415.7961 7169.5 91 3.089 15.7 1 K.FLELHFDIDQAKIAFFQCIKNDSRNSEK.L YER132C 3 3 3.2% 1753 195382 7.8 U PMD1 SGDID:S000000934, Chr V from 430445-425184, reverse complement, Verified ORF, "Protein with an N-terminal kelch-like domain, putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions" * Jamie_Ashy_SPB_19.06811.06811.4 2.0286 0.1291 67.5 2531.2166 2529.8494 4490.7 1 3.215 25.0 1 K.TLSDFEFITADGDSIGVPVYLLR.K * Jamie_Ashy_SPB_40.14294.14294.2 1.5624 0.0060 63.6 1967.7322 1968.0868 6242.6 8 2.671 32.4 1 K.FGNSSQSSNGSLEKYFSK.N * Jamie_Ashy_SPB_12.04069.04069.2 1.9613 0.0685 15.9 1646.2122 1646.8466 4402.9 87 3.34 35.7 1 G.SIAFPSNSGVQNIRR.S YOR191W 3 3 3.2% 1619 184404 7.5 U RIS1 SGDID:S000005717, Chr XV from 692475-697334, Verified ORF, "Member of the SWI/SNF family of DNA-dependent ATPases, plays a role in antagonizing silencing during mating-type switching, contains an N-terminal domain that interacts with Sir4p and a C-terminal SNF2 domain" * Jamie_Ashy_SPB_52.18762.18762.2 1.583 0.0199 63.3 1656.8522 1654.9618 7710.8 250 3.059 34.6 1 K.MPIVFETLQDYGIK.F * Jamie_Ashy_SPB_97.34849.34849.3 2.5035 0.2951 62.0 2943.4143 2947.4353 6803.1 6 4.657 21.3 1 G.GLLADDMGLGKTIQAIALMLANRSEESK.C * Jamie_Ashy_SPB_7.02367.02367.2 2.4308 0.1346 64.1 1309.8322 1310.5822 4089.4 102 3.692 61.1 1 D.ELYSLIRFLR.I Reverse_YDR326C 2 2 3.2% 1438 160578 7.5 U YDR326C SGDID:S000002734, Chr IV from 1124918-1120602, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_107.38290.38290.2 1.1895 0.25 19.3 2706.892 2709.0366 6804.9 125 4.096 19.6 1 K.IHNSLVSNQVTGNGRDILWDWILG.E * Jamie_Ashy_SPB_117.42139.42139.3 2.4395 0.0547 55.7 2488.4043 2488.9338 7134.6 1 3.198 31.0 1 R.RSKKKTSSANSIIKKLDDVLQK.T Reverse_YBR115C 2 2 3.2% 1392 155345 5.9 U LYS2 SGDID:S000000319, Chr II from 473920-469742, reverse complement, Verified ORF, "Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p" * Jamie_Ashy_SPB_86.30735.30735.3 2.5634 0.1361 17.8 2273.6943 2275.474 3999.4 24 3.52 28.9 1 I.PDTEVEKPVDSQFKSLDDPK.D * Jamie_Ashy_SPB_98.35228.35228.2 1.4695 0.2189 28.6 2735.2922 2738.2522 6702.7 1 4.573 25.0 1 L.QDLQGAARIVILGRPKAVGLYITQR.A Reverse_YMR129W 2 2 3.2% 1337 151652 6.7 U POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase;" * Jamie_Ashy_SPB_26.09282.09282.2 1.8929 0.1163 10.5 1695.9722 1693.083 6909.2 196 3.666 42.9 1 P.LKLSAYKTAVQIKKT.S * Jamie_Ashy_SPB_75.26850.26850.4 2.6649 0.0532 63.2 3279.4966 3279.6733 6991.5 245 3.394 20.4 1 K.IPETFLKNSVTDFVIEYNGEKPPSYSFR.N Reverse_YBL105C 2 2 3.2% 1151 131519 7.1 U PKC1 SGDID:S000000201, Chr II from 17696-14241, reverse complement, Verified ORF, "Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)" * Jamie_Ashy_SPB_91.32659.32659.2 1.5958 0.1042 12.5 2465.412 2464.6228 9046.8 2 3.956 31.8 1 R.YKSEMAGGEAASSSRQDGDIQYL.K * Jamie_Ashy_SPB_24.08594.08594.2 1.8374 0.0074 69.5 1736.7322 1736.879 4932.5 3 2.871 42.3 1 K.LSDELYELNQRAER.I Reverse_YKL033W 2 2 3.2% 1038 118889 5.1 U YKL033W SGDID:S000001516, Chr XI from 375099-378215, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YKL033W is an essential gene" * Jamie_Ashy_SPB_23.08030.08030.2 2.2084 0.0501 19.2 1236.1522 1235.3574 5751.0 454 3.233 61.1 1 E.NIMTDRENNK.E * Jamie_Ashy_SPB_89.32034.32034.2 1.6489 0.0661 10.4 2501.7322 2500.869 8479.3 7 3.472 27.3 1 K.AFVSVNGPLIFSLMEGDSIIDQF.L YDL073W 2 2 3.2% 984 113891 7.9 U YDL073W SGDID:S000002231, Chr IV from 326613-329567, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_16.05469.05469.2 2.0274 0.0483 15.0 1667.2322 1667.8595 7702.2 6 3.293 46.4 1 N.FKSLNSSNALITSER.I * Jamie_Ashy_SPB_23.08013.08013.2 1.9376 0.1498 32.8 1946.7722 1948.1454 5834.5 3 4.329 40.0 1 F.SNRLEHDGIFIDFEVR.K YNL313C 2 2 3.2% 904 102336 5.6 U YNL313C SGDID:S000005257, Chr XIV from 48021-45307, reverse complement, Uncharacterized ORF, "Protein required for cell viability" * Jamie_Ashy_SPB_62.22208.22208.2 1.3842 0.1121 13.3 1224.3322 1222.3433 4522.1 266 3.227 38.9 1 K.RQKVDLNEGY.E * Jamie_Ashy_SPB_92.33079.33079.2 1.5373 0.0852 10.3 2116.152 2114.4067 8124.2 23 3.388 27.8 1 V.SIRRDKSGEGSIDLPIIEK.L Reverse_YDR475C 2 2 3.2% 876 98691 9.6 U YDR475C SGDID:S000002883, Chr IV from 1410084-1407454, reverse complement, Uncharacterized ORF, "Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence" * Jamie_Ashy_SPB_5.01742.01742.2 1.6502 0.1024 17.0 1575.5322 1576.8087 5389.3 9 3.642 45.8 1 G.LSLARRTCISRSR.S * Jamie_Ashy_SPB_117.42120.42120.2 1.8322 0.1847 34.1 1687.4922 1689.9487 7445.6 58 4.363 42.9 1 K.TLPTFGDEIPPNFKI.T YGR261C 2 2 3.2% 809 91607 5.8 U APL6 SGDID:S000003493, Chr VII from 1016756-1014327, reverse complement, Verified ORF, "Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools" * Jamie_Ashy_SPB_39.13982.13982.2 1.8202 0.1716 36.3 1179.3722 1177.2225 5717.0 69 4.092 55.0 1 K.SSEGSPRSCPL.P * Jamie_Ashy_SPB_81.28993.28993.2 1.9266 0.0492 12.7 1770.6122 1767.9788 7869.5 1 3.246 42.9 1 K.ELKYYVASAHFPENV.V YNR016C 2 2 3.1% 2233 250351 6.3 U ACC1 SGDID:S000005299, Chr XIV from 661376-654675, reverse complement, Verified ORF, "Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA; required for de novo biosynthesis of long-chain fatty acids" * Jamie_Ashy_SPB_23.08073.08073.4 2.9432 0.1684 16.0 4007.1365 4004.7524 5885.8 231 4.228 16.2 1 R.LRVSSAEIRIIIKDPQTGAPVPLRALINNVSGYVIKT.E * Jamie_Ashy_SPB_28.10051.10051.4 3.0909 0.0961 18.4 3328.5764 3329.7751 5660.6 11 3.004 18.8 1 R.LGGIPLGVIGVETRTVENLIPADPANPNSAETL.I YDR170C 2 2 3.1% 2009 226884 4.8 U SEC7 SGDID:S000002577, Chr IV from 802217-796188, reverse complement, Verified ORF, "Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles" * Jamie_Ashy_SPB_42.15146.15146.4 2.6878 0.1355 17.8 5967.5767 5962.827 9562.6 3 3.462 12.5 1 R.SLSSQNIDADDPTQFENLKLRKTALSECIAIFNNKPKKAIPVLIKKGFLKDDS.P * Jamie_Ashy_SPB_11.03798.03798.2 1.4139 0.0245 63.5 1144.3121 1143.372 5700.8 84 3.122 50.0 1 R.LQLISKGIDR.D Reverse_YCL019W 2 2 3.1% 1770 202095 8.2 U YCL019W SGDID:S000000524, Chr III from 85101-86390,86392-90414, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Ashy_SPB_2.00541.00541.2 2.1635 0.0472 17.7 1243.2922 1242.4606 4468.7 127 2.94 61.1 1 H.PPPRIYLEEK.I Jamie_Ashy_SPB_34.12069.12069.4 2.5192 0.0485 54.0 4767.177 4769.17 9870.5 1 3.307 16.7 1 R.DFEPLNSASVALPDQNSPVEKSTVSAYASGHLSRPNQHLQQSEM.- Reverse_YGL206C 2 2 3.1% 1653 187233 5.2 U CHC1 SGDID:S000003174, Chr VII from 107508-102547, reverse complement, Verified ORF, "Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chains form the clathrin triskelion structural component; the light chain (CLC1) is thought to regulate function" * Jamie_Ashy_SPB_38.13599.13599.3 2.2742 0.183 16.1 2575.5842 2575.9321 9287.4 80 3.379 25.0 1 -.FGTPQVNMASNMLMLPQGDKHEE.N * Jamie_Ashy_SPB_63.22486.22486.4 3.1642 0.1081 15.3 3126.8164 3121.523 6560.3 486 3.335 19.1 1 K.DYHSFINNGLIADAVQPAHLLNVELVRT.Q Reverse_YCL014W 2 2 3.1% 1636 184718 6.2 U BUD3 SGDID:S000000520, Chr III from 96280-101190, Verified ORF, "Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding" * Jamie_Ashy_SPB_60.21321.21321.2 2.0143 0.1888 54.8 1688.9321 1686.9556 7370.0 5 4.01 46.2 1 E.LNKILNENVCLLDK.A * Jamie_Ashy_SPB_98.35142.35142.4 2.6359 0.0797 72.6 4441.7363 4444.279 5895.6 24 3.41 17.1 1 K.LLKEQPGKIISEIKMETYVEREPIVNSFHRIFLKIDK.S Reverse_YAL024C 2 2 3.1% 1435 163149 6.5 U LTE1 SGDID:S000000022, Chr I from 105873-101566, reverse complement, Verified ORF, "Putative GDP/GTP exchange factor required for mitotic exit at low temperatures; acts as a guanine nucleotide exchange factor (GEF) for Tem1p, which is a key regulator of mitotic exit; physically associates with Ras2p-GTP" * Jamie_Ashy_SPB_3.01020.01020.2 1.4021 0.111 10.5 2046.8722 2047.1365 4284.8 282 3.609 28.1 1 L.IDFKSSDSNLFEEEEEK.S * Jamie_Ashy_SPB_79.28147.28147.4 3.4127 0.1343 10.5 3096.6965 3093.4382 4551.8 18 3.934 23.1 1 S.ENDVSPEKAIRHKSYVNSTNDPFLLMS.P YKL129C 2 2 3.1% 1271 142507 9.4 U MYO3 SGDID:S000001612, Chr XI from 200163-196348, reverse complement, Verified ORF, "One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization" Jamie_Ashy_SPB_115.41116.41116.2 1.6241 0.1591 10.6 2194.0923 2191.4575 5412.4 31 4.166 29.4 1 T.LRNNNSSRHGKYLEIKFN.S * Jamie_Ashy_SPB_36.12825.12825.2 1.7132 0.0642 13.4 2322.372 2319.6194 6194.2 486 3.475 25.0 1 K.GDIVFISRDEPSGWSLAKLLD.G Reverse_YKR028W 2 2 3.1% 1098 125063 4.5 U SAP190 SGDID:S000001736, Chr XI from 493900-497196, Verified ORF, "Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p" * Jamie_Ashy_SPB_38.13495.13495.1 1.3295 0.1485 93.8 1792.79 1793.0787 4328.1 6 4.269 30.0 1 K.PRPPTKPTTYLPNGPR.A * Jamie_Ashy_SPB_72.25630.25630.2 1.5259 0.1228 12.4 2134.2722 2136.1528 9103.3 16 3.535 29.4 1 N.EDNDENYLSNGDRHESIK.D YNL163C 2 2 3.1% 1110 124464 5.1 U RIA1 SGDID:S000005107, Chr XIV from 330075-326743, reverse complement, Verified ORF, "Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongation factor 2 (Eft1p and Eft2p)" * Jamie_Ashy_SPB_111.39661.39661.2 1.8582 0.0777 16.4 1541.7522 1538.8771 3965.4 40 3.515 38.5 1 K.IINNKGLKGRSLKP.L * Jamie_Ashy_SPB_62.22273.22273.2 1.9448 0.1879 49.5 2080.652 2080.3984 9263.2 437 4.121 23.7 1 K.ELVPLDVCPSGNIVGIRGLA.G YIL156W 2 2 3.1% 1071 123134 7.9 U UBP7 SGDID:S000001418, Chr IX from 48091-51306, Verified ORF, "Ubiquitin-specific protease that cleaves ubiquitin-protein fusions" * Jamie_Ashy_SPB_94.33546.33546.2 1.8264 0.1234 17.7 1501.3121 1502.6912 5078.4 287 3.716 38.5 1 K.MYLNGGCSVVPTGF.L * Jamie_Ashy_SPB_41.14667.14667.2 1.7362 0.0639 13.5 2006.1721 2004.2883 6896.3 429 3.246 30.6 1 S.VSTSVSALENEPSIVKSKK.K YPL150W 2 2 3.1% 901 100646 9.4 U YPL150W SGDID:S000006071, Chr XVI from 268187-270892, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_23.08018.08018.2 2.6285 0.0087 10.1 1295.5322 1295.3428 5730.3 22 2.709 63.6 1 N.TDSIIQEVSSSE.E * Jamie_Ashy_SPB_49.17382.17382.2 1.4692 0.1059 13.5 1779.5122 1782.05 7726.3 247 3.124 30.0 1 T.PVLQTKGLIENGLNER.D YKL211C 2 2 3.1% 484 53489 6.9 U TRP3 SGDID:S000001694, Chr XI from 38154-36700, reverse complement, Verified ORF, "Bifunctional enzyme exhibiting both indole-3-glycerol-phosphate synthase and anthranilate synthase activities, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p" * Jamie_Ashy_SPB_4.01186.01186.2 1.7125 0.0969 17.3 855.47217 856.03064 3723.1 35 3.244 75.0 1 E.EGHLMIR.N * Jamie_Ashy_SPB_5.01668.01668.2 1.8302 0.0293 12.3 958.03217 957.11786 6186.2 308 3.706 64.3 1 S.LQDLVNVR.K YGR032W 3 3 3.0% 1895 216988 7.0 U GSC2 SGDID:S000003264, Chr VII from 548268-553955, Verified ORF, "Catalytic subunit of 1,3-beta-glucan synthase, has similarity to an alternate catalytic subunit, Fks1p (Gsc1p); Rho1p encodes the regulatory subunit; involved in cell wall synthesis and maintenance" * Jamie_Ashy_SPB_23.08055.08055.2 1.5605 0.1699 18.9 2695.2122 2697.936 3978.9 5 3.982 29.2 1 R.SSRMSPEQALLSLHADYIGGDTANY.K * Jamie_Ashy_SPB_44.15640.15640.2 1.7993 0.0557 13.1 3004.372 3005.424 6380.2 78 2.902 20.4 1 L.AQIGGKLHYGHPDFINATFMTTRGGVSK.A Jamie_Ashy_SPB_60.21316.21316.4 2.4684 0.111 75.7 2782.9365 2778.1606 5792.8 434 3.4 25.0 1 K.LHYGHPDFINATFMTTRGGVSKAQK.G YIL177C 4 4 3.0% 1758 197511 7.4 U YIL177C SGDID:S000001439, Chr IX from 6147-4987,4598-483, reverse complement, Uncharacterized ORF, "Hypothetical protein" YJL225C 4 4 3.0% 1758 197564 7.5 U YJL225C SGDID:S000003760, Chr X from 6130-4970,4581-466, reverse complement, Uncharacterized ORF, "Hypothetical protein" Jamie_Ashy_SPB_13.04689.04689.4 2.8603 0.1104 17.5 3880.3765 3875.5923 6213.9 372 3.533 17.7 1 R.KPIEIKYFSQICNDMMNKKDRLGDILHIILRA.C Jamie_Ashy_SPB_15.05271.05271.2 2.1705 0.0852 94.2 1680.4922 1679.9125 4191.2 346 3.112 41.7 1 K.YFSQICNDMMNKK.D Jamie_Ashy_SPB_11.03794.03794.2 1.6601 0.0384 12.8 882.4522 882.99207 4627.0 10 3.069 66.7 1 T.TLKDSYR.I Jamie_Ashy_SPB_5.01737.01737.3 2.9427 0.1815 45.6 1664.8444 1667.8254 5006.5 68 4.866 34.6 1 R.FDILLCRDSSREVG.E Reverse_YOR191W 2 2 3.0% 1619 184405 7.5 U RIS1 SGDID:S000005717, Chr XV from 692475-697334, Verified ORF, "Member of the SWI/SNF family of DNA-dependent ATPases, plays a role in antagonizing silencing during mating-type switching, contains an N-terminal domain that interacts with Sir4p and a C-terminal SNF2 domain" * Jamie_Ashy_SPB_76.27228.27228.4 3.2396 0.1488 10.1 4341.5366 4344.7285 7244.5 352 3.534 14.9 1 I.ISESQKVNELLARIQEQDEAAHMNSIHFEDQMSLDNAT.S * Jamie_Ashy_SPB_21.07260.07260.2 1.6496 0.1024 15.7 1187.5922 1187.2579 4872.4 73 3.713 50.0 1 K.SVYSSSIGCIN.E YBL034C 4 4 3.0% 1513 174176 6.3 U STU1 SGDID:S000000130, Chr II from 158392-153851, reverse complement, Verified ORF, "Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles" * Jamie_Ashy_SPB_108.38715.38715.1 1.158 0.221 15.4 1272.81 1271.4125 4827.8 291 4.435 30.0 1 E.LVNESSIQAYF.A * Jamie_Ashy_SPB_103.36961.36961.2 1.4025 0.053 64.0 2058.2522 2057.3115 8274.3 300 2.84 26.5 1 K.LSNKLKTNLRSTSEYSSK.E * Jamie_Ashy_SPB_53.18824.18824.2 1.6895 0.077 13.3 1488.0922 1486.622 6694.5 238 3.111 41.7 1 K.LKTNLRSTSEYSS.K * Jamie_Ashy_SPB_43.15230.15230.2 1.8342 0.0516 12.6 1904.9922 1903.9977 6731.5 130 3.12 31.2 1 T.SSTDSVVIHDDNDKDKK.L Reverse_YHR164C 3 3 3.0% 1522 171694 6.5 U DNA2 SGDID:S000001207, Chr VIII from 429180-424612, reverse complement, Verified ORF, "Essential tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair pathways; potential Cdc28p substrate" * Jamie_Ashy_SPB_8.02784.02784.3 2.3127 0.0358 56.3 1416.8043 1416.6664 4055.7 12 3.149 45.5 1 R.FKPARNDVLLSR.L * Jamie_Ashy_SPB_25.08938.08938.3 2.133 0.1724 12.9 4065.8943 4067.749 4397.8 2 4.183 19.7 1 K.EKDLLRNNLLKRKVSIGIKAPNIFQVRGQCLCFH.G * Jamie_Ashy_SPB_5.01740.01740.4 1.6648 0.0346 33.1 1672.2166 1671.0391 3615.8 106 2.718 32.1 1 R.KVSIGIKAPNIFQVR.G YNL273W 2 2 3.0% 1238 141120 6.1 U TOF1 SGDID:S000005217, Chr XIV from 122883-126599, Verified ORF, "Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase" * Jamie_Ashy_SPB_73.26294.26294.2 1.7413 0.0714 12.2 1861.3322 1859.281 5642.6 108 3.143 31.2 1 K.TILSMESGKVLRAAIRL.A * Jamie_Ashy_SPB_72.25834.25834.2 1.7066 0.044 73.0 2052.4922 2052.2517 8217.7 12 3.348 34.2 1 K.HQSVAAEGLPNSLLNSQTGK.A Reverse_YHR158C 2 2 3.0% 1164 131094 5.4 U KEL1 SGDID:S000001201, Chr VIII from 417182-413688, reverse complement, Verified ORF, "Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate" * Jamie_Ashy_SPB_31.10902.10902.2 1.7609 0.0525 10.5 1838.3121 1840.135 7008.2 379 2.95 28.1 1 R.GLPRPGVPAPVTWKYSN.I * Jamie_Ashy_SPB_8.02570.02570.2 1.4738 0.2595 49.1 1981.3322 1979.2034 8654.9 111 4.142 29.4 1 K.SLQKHLTTSPGSVNRQQP.P YDR507C 2 2 3.0% 1142 129858 9.2 U GIN4 SGDID:S000002915, Chr IV from 1465776-1462348, reverse complement, Verified ORF, "Protein kinase involved in bud growth and assembly of the septin ring, proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Kcc4p and Hsl1p" * Jamie_Ashy_SPB_10.03357.03357.2 1.1669 0.0578 61.8 2190.7322 2193.6716 4655.2 93 3.582 19.4 1 R.SIKRMSLIPSMKRESVTTK.L * Jamie_Ashy_SPB_3.00913.00913.2 1.6757 0.0222 67.5 1634.7122 1636.7648 4957.9 187 3.339 42.9 1 K.NAGSQAKDHSKDHLK.E Reverse_YDL231C 2 2 3.0% 1125 129725 9.0 U BRE4 SGDID:S000002390, Chr IV from 42245-38868, reverse complement, Verified ORF, "Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport" * Jamie_Ashy_SPB_83.29653.29653.2 1.6883 0.0803 11.4 2207.112 2204.5417 7523.1 25 3.803 31.2 1 R.SLKWLKFRWPNQTTHFN.F * Jamie_Ashy_SPB_21.07592.07592.2 1.6494 0.1238 14.4 1806.2322 1805.9794 8434.5 140 3.569 34.4 1 R.LSTQSYSGKVIDPDPTP.S YIL112W 2 2 3.0% 1083 123556 5.7 U HOS4 SGDID:S000001374, Chr IX from 151592-154843, Uncharacterized ORF, "Subunit of the Set3 complex, which is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate" * Jamie_Ashy_SPB_80.28714.28714.4 2.7091 0.1113 15.8 2707.0166 2709.1013 4282.5 132 3.804 26.2 1 V.DIQNHQPFSRDQLRAMLKEPKR.K * Jamie_Ashy_SPB_1.00295.00295.2 1.4887 0.091 13.8 1314.2122 1312.6449 2368.8 168 3.479 44.4 1 D.QKILREIKKR.L YNL132W 2 2 3.0% 1056 119347 7.8 U KRE33 SGDID:S000005076, Chr XIV from 375323-378493, Verified ORF, "Essential protein of unknown function; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance" * Jamie_Ashy_SPB_30.10777.10777.2 1.9716 0.1735 43.1 1798.3522 1798.1765 6246.1 150 4.035 31.2 1 A.RTIETVEGGGIVVILLK.S * Jamie_Ashy_SPB_62.22333.22333.2 1.58 0.08 75.1 1840.7722 1839.971 9109.1 397 3.309 32.1 1 R.DYFEGKFTDMSEDVR.P YML093W 2 2 3.0% 899 103023 5.1 U UTP14 SGDID:S000004558, Chr XIII from 83090-85789, Verified ORF, "Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA" * Jamie_Ashy_SPB_58.20605.20605.4 2.56 0.1134 15.1 2270.5366 2275.8198 4994.2 45 3.66 30.6 1 S.KNVKAMNKMEKAELKQKKK.K * Jamie_Ashy_SPB_35.12306.12306.2 1.4605 0.1933 28.6 1008.8722 1006.14905 6090.3 2 4.072 78.6 1 A.VPFPFENR.E Reverse_YLR087C 4 4 2.9% 2958 338258 5.9 U CSF1 SGDID:S000004077, Chr XII from 315732-306856, reverse complement, Verified ORF, "Protein required for fermentation at low temperature" * Jamie_Ashy_SPB_87.31116.31116.4 3.1806 0.1676 19.8 4520.5767 4524.055 10294.0 252 3.784 14.5 1 H.KISAKTHELTLSFELPQESDFENTAMSFLFSEFGVDAQVK.A * Jamie_Ashy_SPB_77.27415.27415.2 1.5596 0.1668 18.8 2679.0723 2681.1597 8048.4 19 4.036 25.0 1 R.EKILFLINEISFQGTVKTEEPLM.G * Jamie_Ashy_SPB_51.18318.18318.2 2.216 0.1696 56.7 2176.9722 2179.518 5556.8 1 3.999 38.9 1 K.ILFLINEISFQGTVKTEEP.L * Jamie_Ashy_SPB_116.41803.41803.2 1.4385 0.1903 20.3 2721.5723 2720.1448 6892.6 3 4.737 21.7 1 L.KLLRDSFKGKVSGVRGTIEITEER.N Reverse_YLR430W 3 3 2.9% 2231 252495 8.6 U SEN1 SGDID:S000004422, Chr XII from 993430-1000125, Verified ORF, "Nuclear protein, putative helicase required for processing of tRNAs, rRNAs, and small nuclear RNAs; potential Cdc28p substrate" * Jamie_Ashy_SPB_16.05667.05667.2 1.5224 0.0886 10.6 1378.5521 1379.4692 5882.1 6 3.559 46.4 1 R.TGTPGSGPGKFASSQ.P * Jamie_Ashy_SPB_6.02126.02126.3 1.5106 0.0124 33.5 3106.9744 3109.5125 6447.0 6 2.995 16.4 1 K.ANASSQFTGFSTIKPQAVRPRGTISSSLAK.R * Jamie_Ashy_SPB_15.05230.05230.2 1.8667 0.1209 17.5 1865.3322 1866.9739 5145.3 13 3.36 36.8 1 R.SEASSSAITSSLSPSLSPSA.P YAL029C 2 2 2.9% 1471 169343 7.6 U MYO4 SGDID:S000000027, Chr I from 92271-87856, reverse complement, Verified ORF, "One of two type V myosins; required for mother-specific HO expression, for the bud tip localization of ASH1 and IST2 mRNA; facilitates growth and orientation of ER tubules along with She3p" * Jamie_Ashy_SPB_50.17841.17841.4 2.7005 0.0602 67.2 2963.7366 2960.3801 6271.6 271 3.199 21.7 1 K.RKDELEPHLFAIAEEAYRFMVHEK.A * Jamie_Ashy_SPB_15.05338.05338.2 1.574 0.1225 17.7 2345.5522 2344.5913 5321.1 14 3.952 26.5 1 K.SRLVYQPETERNYHIFYQ.I Reverse_YDL112W 2 2 2.9% 1436 165047 6.1 U TRM3 SGDID:S000002270, Chr IV from 258915-263225, Verified ORF, "2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs" * Jamie_Ashy_SPB_41.14664.14664.3 2.835 0.1639 11.4 2405.2744 2408.7659 5711.2 402 3.872 25.0 1 W.RDATVAVNKFQPHNKVKIDQV.T * Jamie_Ashy_SPB_73.26239.26239.2 1.6343 0.1217 15.9 2369.0322 2370.71 8738.0 283 3.673 23.7 1 L.QASTRLAKNMEARSSRFMER.A Reverse_YIL115C 2 2 2.9% 1460 158907 4.8 U NUP159 SGDID:S000001377, Chr IX from 148706-144324, reverse complement, Verified ORF, "Subunit of the nuclear pore complex that is found exclusively on the cytoplasmic side, forms a subcomplex with Nup82p and Nsp1p, required for mRNA export" * Jamie_Ashy_SPB_36.12666.12666.3 2.9547 0.0776 11.6 2442.4443 2442.487 8797.2 482 3.444 25.0 1 S.EDRDSNLSMNDDSEKVESSQR.S * Jamie_Ashy_SPB_59.21117.21117.2 1.795 0.0993 14.9 2054.0322 2056.058 6396.4 9 3.728 31.0 1 K.ASAPDTADQESSAVGTSTSSVS.P Reverse_YCR033W 2 2 2.9% 1226 138397 9.1 U SNT1 SGDID:S000000629, Chr III from 186484-190164, Verified ORF, "Subunit of the Set3C deacetylase complex; putative DNA-binding protein" * Jamie_Ashy_SPB_48.17160.17160.3 2.2735 0.1039 69.0 2517.8943 2518.7422 7638.2 360 3.256 23.8 1 R.PPFNNDHYSIAKTEDKSPTEVK.V * Jamie_Ashy_SPB_67.24076.24076.2 1.6881 0.1196 89.2 1486.1522 1488.7478 4725.5 20 3.413 41.7 1 K.KDGLRRTPPPYGM.- Reverse_YJR089W 2 2 2.9% 954 108667 6.5 U BIR1 SGDID:S000003849, Chr X from 587632-590496, Verified ORF, "Protein involved in cell cycle regulation and chromosome segregation, contains BIR (Baculovirus Inhibitor of apoptosis Repeat) domain" * Jamie_Ashy_SPB_29.10138.10138.2 1.9792 0.1106 93.8 1689.0922 1687.928 7740.2 80 3.626 39.3 1 K.KIEVTSSDIPIDLEK.G * Jamie_Ashy_SPB_28.10084.10084.2 1.8093 0.149 95.1 1531.6322 1533.7709 5057.2 265 4.131 45.8 1 K.KELAVGDQFTRLR.N Reverse_YGR162W 2 2 2.9% 952 107101 6.0 U TIF4631 SGDID:S000003394, Chr VII from 824064-826922, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4632p" * Jamie_Ashy_SPB_25.08881.08881.2 1.8632 0.0824 15.8 1542.2322 1543.7576 5188.2 459 3.792 42.3 1 E.DKDLLETKGDPALK.K * Jamie_Ashy_SPB_44.15591.15591.2 1.9247 0.0182 74.8 1482.1721 1482.7875 7677.0 80 3.062 46.2 1 R.SRNGRGMGPPIVIK.S YDL040C 2 2 2.9% 854 98905 8.8 U NAT1 SGDID:S000002198, Chr IV from 381435-378871, reverse complement, Verified ORF, "Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing" * Jamie_Ashy_SPB_3.00910.00910.2 1.5944 0.0021 63.6 1601.4722 1602.9823 6060.0 224 2.62 34.6 1 K.KKAKKEAAAMNKRK.E * Jamie_Ashy_SPB_15.05326.05326.1 1.2201 0.2855 50.0 1181.33 1182.2755 3655.5 2 4.963 45.0 1 I.SSLSPLEPHSQ.N Reverse_YGR184C 2 2 2.8% 1950 224837 5.5 U UBR1 SGDID:S000003416, Chr VII from 865758-859906, reverse complement, Verified ORF, "Ubiquitin-protein ligase (E3) that interacts with Rad6p/Ubc2p to ubiquitinate substrates of the N-end rule pathway; binds to the Rpn2p, Rpt1p, and Rpt6p proteins of the 19S particle of the 26S proteasome" * Jamie_Ashy_SPB_17.05882.05882.3 2.9551 0.1311 13.5 2862.7444 2864.3545 7723.5 311 4.604 22.8 1 R.LKITIDKQITPLPEINQNFVDIFY.D * Jamie_Ashy_SPB_111.39926.39926.3 1.3038 0.0108 32.9 3635.4844 3634.1099 7217.0 213 3.078 12.5 1 R.TYRFYPLNHISRLTRRIHGQLSGLDDDAVSM.- contaminant_IGA4_HAEIN 2 2 2.8% 1849 202956 5.7 U owl|P45386| IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE).... * Jamie_Ashy_SPB_25.08796.08796.2 1.5475 0.0895 12.2 1031.1522 1032.1387 4867.3 17 3.339 55.6 1 S.ATVSTEQPAK.E * Jamie_Ashy_SPB_86.30668.30668.4 2.9667 0.1326 13.8 4775.8965 4772.4614 7858.9 15 3.727 15.4 1 K.INVSVYDFAYNVENQQQYNAGLKLKYHNVKLSLIGGLTKAKQ.A Reverse_YDR093W 2 2 2.8% 1612 182618 6.2 U DNF2 SGDID:S000002500, Chr IV from 631277-636115, Verified ORF, "Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the plasma membrane and late exocytic or early endocytic membranes, likely involved in protein transport; potential Cdc28p substrate" * Jamie_Ashy_SPB_72.25783.25783.4 3.3903 0.1372 10.1 3789.0964 3788.4102 9622.8 106 3.638 18.4 1 Y.PFFFCIVSQYVGDLMYWLFKTQNWEKRLIG.V * Jamie_Ashy_SPB_48.17319.17319.2 1.8737 0.1562 28.7 1510.0721 1510.7336 6675.5 118 3.847 35.7 1 R.KLVSGGTKAVEINGH.A Reverse_YDR217C 2 2 2.8% 1309 148397 5.3 U RAD9 SGDID:S000002625, Chr IV from 903474-899545, reverse complement, Verified ORF, "DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate" * Jamie_Ashy_SPB_29.10346.10346.2 1.6825 0.1499 10.4 2051.872 2049.112 7777.7 219 3.473 26.5 1 Y.IDNDTIDDHFGTDENIIT.Q * Jamie_Ashy_SPB_19.06550.06550.2 1.9449 0.1474 28.7 1799.1122 1799.9811 4733.3 1 3.865 41.2 1 E.GSSFLLSPAGAHEALADR.S YKL015W 2 2 2.8% 979 111414 5.4 U PUT3 SGDID:S000001498, Chr XI from 408187-411126, Verified ORF, "Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences and undergoes a conformational change to form the active state; has a Zn(2)-Cys(6) binuclear cluster domain" * Jamie_Ashy_SPB_75.26914.26914.2 1.7888 0.0699 12.9 1933.8922 1933.2732 7042.7 377 3.004 28.1 1 T.IVNLNSINDNLSPLMFK.I * Jamie_Ashy_SPB_41.14779.14779.2 1.4281 0.1123 12.7 1266.4122 1266.3965 5440.1 123 3.064 50.0 1 T.QKDEDFKISR.L YPL082C 2 2 2.7% 1867 209975 6.6 U MOT1 SGDID:S000006003, Chr XVI from 404080-398477, reverse complement, Verified ORF, "Essential abundant protein involved in regulation of transcription, removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity" * Jamie_Ashy_SPB_22.07663.07663.2 2.1171 0.1302 27.7 1549.9922 1548.653 5790.6 2 3.19 50.0 1 A.SDKANENKSNANKK.S * Jamie_Ashy_SPB_19.06745.06745.4 2.4242 0.0058 40.5 4023.3364 4019.6404 8765.3 29 3.171 17.1 1 K.LNNSTIEILVTTIWSLLARLDDDISSSVGSIMDLLAK.L Reverse_YHR214C-B 3 3 2.7% 1793 202816 8.3 U YHR214C-B SGDID:S000003534, Chr VIII from 549346-547931,547929-543964, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Ashy_SPB_2.00541.00541.2 2.1635 0.0472 17.7 1243.2922 1242.4606 4468.7 127 2.94 61.1 1 H.PPPRIYLEEK.I * Jamie_Ashy_SPB_51.18199.18199.2 1.702 0.0574 10.5 2495.2322 2496.9888 5248.1 152 2.884 26.3 1 W.TKMMLLQNVEKHYAQIYKEK.E Jamie_Ashy_SPB_85.30410.30410.2 1.7425 0.0648 12.8 2085.8123 2083.324 8326.3 401 3.003 27.8 1 H.SSEHTNSQSMPALLPVDLR.H Reverse_contaminant_IGA0_HAEIN 2 2 2.7% 1694 185538 6.4 U owl|P44969| IMMUNOGLOBULIN A1 PROTEASE PRECURSOR (EC 3.4.21.72) (IGA1 PROTEASE).... * Jamie_Ashy_SPB_21.07438.07438.3 2.7608 0.0509 66.7 2602.6443 2604.756 11416.1 175 3.733 21.7 1 R.RRRSKPDSSESNNAVSNDAVTNAK.H Jamie_Ashy_SPB_49.17608.17608.2 1.8109 0.0747 12.3 2225.0322 2226.4575 6133.7 233 3.272 25.0 1 I.NNNLTLTGSGRLTVSKGHNNK.K Reverse_YGL173C 2 2 2.7% 1528 175459 7.5 U KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonuclease involved in mRNA decay, evolutionarily conserved component of cytoplasmic processing (P) bodies, plays a role in microtubule-mediated processes, filamentous growth, and ribosomal RNA maturation" * Jamie_Ashy_SPB_22.07758.07758.3 2.7941 0.1019 82.1 2123.4243 2122.3826 4829.9 29 3.652 29.2 1 K.EDVFSILVVAEWDATKGNK.D * Jamie_Ashy_SPB_84.29976.29976.2 1.5456 0.105 11.8 2661.7122 2662.0845 6495.7 86 3.23 27.3 1 K.LHRIFNMIKHEGEGPVEHGSFII.Q Reverse_YJR092W 2 2 2.7% 1448 165023 5.0 U BUD4 SGDID:S000003852, Chr X from 598649-602995, Verified ORF, "Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding; potential Cdc28p substrate" * Jamie_Ashy_SPB_16.05491.05491.4 3.1259 0.1763 37.5 2400.3765 2401.598 5189.0 338 3.879 28.9 1 I.NEEPSKTRQGYLSEEHQVIR.Y * Jamie_Ashy_SPB_64.22861.22861.2 1.8863 0.124 20.5 2077.3523 2077.4038 7394.0 10 3.753 33.3 1 E.SNVRLLGLNLSENSITSMK.S Reverse_YNL250W 2 2 2.7% 1312 152568 6.1 U RAD50 SGDID:S000005194, Chr XIV from 175411-179349, Verified ORF, "Subunit of MRX complex, with Mre11p and Xrs2p, involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining" * Jamie_Ashy_SPB_7.02376.02376.4 2.8833 0.0948 15.7 2588.4966 2593.035 4469.5 15 3.292 26.7 1 D.KEMQLDSITKRLERLSDNMKR.Q * Jamie_Ashy_SPB_29.10458.10458.2 1.5056 0.0269 64.0 1769.1122 1770.9371 6228.8 422 2.816 32.1 1 K.EALEESDHILKSRDK.K Reverse_YMR128W 2 2 2.7% 1267 144954 6.3 U ECM16 SGDID:S000004735, Chr XIII from 523695-527498, Verified ORF, "Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis" * Jamie_Ashy_SPB_13.04518.04518.2 1.8691 0.0956 16.8 1310.6122 1310.4467 5034.8 197 3.337 55.0 1 K.KEKEEGTITQF.G * Jamie_Ashy_SPB_68.24392.24392.2 1.5502 0.2226 28.5 2706.5322 2706.9346 9354.1 113 4.668 22.7 1 E.FSQVGNSENYKREKSRGSDVVYR.V YKR103W 2 2 2.7% 1218 137996 8.8 U NFT1 SGDID:S000001811, Chr XI from 652718-656374, Verified ORF, "Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds." * Jamie_Ashy_SPB_90.32092.32092.2 1.7027 0.0565 13.4 1431.5322 1433.7087 4530.0 26 3.384 50.0 1 L.MPRILSYVNDPK.S * Jamie_Ashy_SPB_18.06189.06189.2 1.8857 0.1023 16.7 2132.2722 2133.3286 8105.0 229 3.497 27.5 1 T.DVGDGGFSLSGGQQQRIALAR.A Reverse_YFR030W 2 2 2.7% 1035 114829 5.3 U MET10 SGDID:S000001926, Chr VI from 213300-216407, Verified ORF, "Subunit alpha of assimilatory sulfite reductase, which is responsible for the conversion of sulfite into sulfide" * Jamie_Ashy_SPB_18.06441.06441.2 2.062 0.2156 70.2 1550.5721 1552.7739 6273.7 41 3.92 45.8 1 H.VKQSSAISYERRK.L * Jamie_Ashy_SPB_27.09669.09669.2 2.0251 0.0853 15.6 1760.3922 1761.969 6883.3 114 3.282 39.3 1 L.VVDSENLGYFTLFEK.V YMR275C 2 4 2.7% 976 109174 8.7 U BUL1 SGDID:S000004888, Chr XIII from 818580-815650, reverse complement, Verified ORF, "Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex, functional homolog of Bul2p, disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases" * Jamie_Ashy_SPB_53.18918.18918.2 1.7966 0.0782 12.9 1904.5922 1903.8766 7878.9 100 4.26 29.4 1 H.DLDNGRSGNSGHNTDTSR.A * Jamie_Ashy_SPB_9.03222.03222.2 1.794 0.0955 17.8 932.09216 932.06665 2553.7 8 3.571 71.4 3 K.QGLPYWAP.S YDR484W 2 2 2.7% 641 74333 5.8 U VPS52 SGDID:S000002892, Chr IV from 1422753-1424678, Verified ORF, "Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin" * Jamie_Ashy_SPB_36.12812.12812.2 1.8708 0.068 13.1 1896.9521 1895.0942 6920.3 115 3.27 31.2 1 D.NKDAENSAMLAPKDFDK.L * Jamie_Ashy_SPB_33.11605.11605.2 1.8134 0.0864 15.8 1466.7322 1466.6489 5833.6 121 3.352 41.7 1 A.ENSAMLAPKDFDK.L YNL271C 2 2 2.6% 1953 219701 6.5 U BNI1 SGDID:S000005215, Chr XIV from 135384-129523, reverse complement, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1" * Jamie_Ashy_SPB_72.25897.25897.4 2.9202 0.0911 11.8 3421.1765 3422.6118 6447.4 283 3.455 18.9 1 K.GFGIDFNVDEIMDSPKKNTGDVETEEDANYA.S * Jamie_Ashy_SPB_48.17077.17077.4 2.8375 0.0993 17.9 2589.0566 2594.0806 4004.0 2 3.569 29.8 1 P.SLFEKYPRPHKKLKQLHWEK.L Reverse_YKR095W 2 2 2.6% 1875 218455 5.2 U MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs" * Jamie_Ashy_SPB_41.14737.14737.3 2.9034 0.1654 10.5 2976.9543 2977.1682 7086.7 45 4.271 20.4 1 E.EDNTDTSLTSSSSILGRSAPINFSPELH.T * Jamie_Ashy_SPB_20.07215.07215.4 2.7124 0.1573 11.6 2609.6965 2610.0696 5164.0 18 3.155 25.0 1 K.ELEEKKREIIKNIKEETPLRI.R YHL009W-B 2 2 2.6% 1802 207969 8.4 U YHL009W-B SGDID:S000007372, Chr VIII from 85905-86990,86992-91314, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Ashy_SPB_52.18736.18736.3 2.8612 0.1407 14.8 2949.3542 2947.368 7204.4 75 3.98 24.0 1 K.LSETDDIQMLAEKLLKLGETIDKVEN.R Jamie_Ashy_SPB_90.32161.32161.3 2.2231 0.0052 39.0 2523.2043 2518.7952 6081.3 402 2.618 27.6 1 R.KRHDKNNSLTSYELERDKKR.S YER164W 2 2 2.6% 1468 168240 6.5 U CHD1 SGDID:S000000966, Chr V from 505387-509793, Verified ORF, "Nucleosome remodeling factor that functions in regulation of transcription elongation; contains a chromo domain, a helicase domain and a DNA-binding domain; component of both the SAGA and SILK complexes" * Jamie_Ashy_SPB_52.18421.18421.4 3.1615 0.1752 31.0 2428.7766 2423.7747 4740.1 368 4.326 27.0 1 R.RVDYLLSFLRGGLNTKSPSADI.G * Jamie_Ashy_SPB_2.00626.00626.4 2.7375 0.121 14.8 1939.4565 1938.1527 3618.8 73 3.416 30.0 1 K.ASPEKYRKHLWSYSAN.F YCR042C 2 2 2.6% 1407 161470 5.7 U TAF2 SGDID:S000000638, Chr III from 205392-201169, reverse complement, Verified ORF, "TFIID subunit (150 kDa), involved in RNA polymerase II transcription initiation" * Jamie_Ashy_SPB_74.26478.26478.2 1.8694 0.1025 90.1 1867.6122 1869.2506 6644.9 65 3.81 33.3 1 R.MTKTERSFGMSRVLPK.I * Jamie_Ashy_SPB_99.35630.35630.3 2.8282 0.1443 12.2 2527.4343 2523.0117 4393.9 8 4.37 27.5 1 H.DLVRVMYTLINSFVVRLETPR.E Reverse_YPR164W 2 2 2.6% 1407 161237 5.9 U MMS1 SGDID:S000006368, Chr XVI from 870699-874922, Verified ORF, "Protein likely involved in protection against replication-dependent DNA damage; mutants are sensitive to methyl methanesulfonate (MMS); implicated in regulation of Ty1 transposition" * Jamie_Ashy_SPB_96.34600.34600.2 1.4578 0.0062 61.3 2359.4521 2361.9146 7460.9 322 3.444 21.4 1 K.NKAATLLVLKLSSTFSILLVTK.K * Jamie_Ashy_SPB_38.13417.13417.2 1.6494 0.0857 12.2 1457.5721 1459.6426 5840.9 169 3.257 42.3 1 L.LQSDLGVAKGDSLR.I YDR104C 2 2 2.6% 1245 145181 9.1 U SPO71 SGDID:S000002511, Chr IV from 664905-661168, reverse complement, Verified ORF, "Meiosis-specific protein of unknown function, required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis" * Jamie_Ashy_SPB_119.42570.42570.2 1.5116 0.1271 13.0 1958.3922 1961.3574 10777.5 279 4.155 30.0 1 K.IFQHPILRYFTVAILE.K * Jamie_Ashy_SPB_99.35353.35353.2 1.7038 0.1089 15.4 2011.8922 2009.3651 7046.5 14 3.705 36.7 1 S.HLIQYRPIANRLRETK.S YHR165C 2 2 2.5% 2413 279502 7.3 U PRP8 SGDID:S000001208, Chr VIII from 436950-429709, reverse complement, Verified ORF, "Component of the U4/U6-U5 snRNP complex, involved in the second catalytic step of splicing" * Jamie_Ashy_SPB_17.05832.05832.2 2.0268 0.042 14.8 1390.5922 1390.5762 6558.4 275 3.376 50.0 1 P.PPPPPGLDELETK.A * Jamie_Ashy_SPB_82.29496.29496.4 2.3273 0.1698 17.7 6044.6963 6046.6255 8520.2 92 4.1 12.8 1 R.DRHTLAYDRGHRIRREFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTD.V YJL207C 2 2 2.5% 2014 229898 5.7 U YJL207C SGDID:S000003743, Chr X from 47433-41389, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_74.26370.26370.2 1.3609 0.0014 61.0 2302.2522 2299.5085 7371.6 34 2.959 27.8 1 K.VFLNNCPEYLSETIENSLR.A * Jamie_Ashy_SPB_61.21799.21799.3 3.2958 0.2104 60.3 3170.6643 3168.6907 7475.4 23 4.859 20.8 1 P.AFSKGSSPTVMSFAITVAAEVLASNIMPPDK.L YJL005W 3 3 2.5% 2026 227832 7.3 U CYR1 SGDID:S000003542, Chr X from 425072-431152, Verified ORF, "Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; involved in cell cycle control and glucose and nitrogen repression of sporulation" * Jamie_Ashy_SPB_37.13304.13304.2 1.7328 0.1175 18.0 1495.4722 1493.6658 5942.2 224 3.695 41.7 1 S.NHLADNVPSTLRR.K * Jamie_Ashy_SPB_72.25662.25662.2 1.7018 0.098 17.8 1814.8922 1813.964 7577.5 154 3.703 34.6 1 L.EHYYKDFSDLDPKR.H * Jamie_Ashy_SPB_55.19790.19790.3 2.7238 0.1417 16.9 2809.0144 2808.2908 8072.1 2 3.779 28.3 1 T.NLLQIDLSYNKIQSLPQSTKYLVK.L YDR098C-B 2 2 2.5% 1755 198594 8.4 U YDR098C-B SGDID:S000007391, Chr IV from 651121-649817,649815-645853, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YGR038C-B 2 2 2.5% 1755 198520 8.2 U YGR038C-B SGDID:S000007408, Chr VII from 567471-566167,566165-562203, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YER160C 2 2 2.5% 1755 198568 8.5 U YER160C SGDID:S000000962, Chr V from 498119-496818,496816-492851, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YER138C 2 2 2.5% 1755 198561 8.1 U YER138C SGDID:S000000940, Chr V from 449020-447719,447717-443752, reverse complement, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YDR316W-B 2 2 2.5% 1755 198526 8.2 U YDR316W-B SGDID:S000007399, Chr IV from 1096059-1097363,1097365-1101327, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" Jamie_Ashy_SPB_9.03136.03136.2 1.5414 0.0151 64.0 2076.612 2076.098 4762.2 143 4.18 31.6 1 R.AHNVSTSNNSPSTDNDSISK.S Jamie_Ashy_SPB_21.07315.07315.4 3.3519 0.1003 11.8 2463.1365 2467.568 5510.6 272 3.67 22.5 1 I.NSHQTNSSLGGIGDSNAYTTINSK.K YOR336W 3 3 2.5% 1365 156477 5.2 U KRE5 SGDID:S000005863, Chr XV from 949768-953865, Verified ORF, "Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects" * Jamie_Ashy_SPB_32.11517.11517.2 1.6962 0.0725 13.4 1315.9722 1313.4069 6065.0 23 3.554 50.0 1 P.FSSDSDKSVVNK.I * Jamie_Ashy_SPB_92.32942.32942.2 1.4033 0.045 64.3 2575.7522 2577.9004 9401.5 59 3.281 26.2 1 K.DTEFIRKELSNSSPKNKQISVR.D * Jamie_Ashy_SPB_15.05096.05096.2 2.1485 0.0103 84.3 1232.4122 1232.3794 4221.3 262 2.742 65.0 1 R.KELSNSSPKNK.Q YMR076C 2 2 2.5% 1277 147041 6.3 U PDS5 SGDID:S000004681, Chr XIII from 420028-416195, reverse complement, Verified ORF, "Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes in an interdependent manner, may function as a protein-protein interaction scaffold" * Jamie_Ashy_SPB_64.23007.23007.2 1.7213 0.0844 12.4 1833.0122 1831.1626 4993.7 210 3.011 37.5 1 R.ASPIIYNVSNISVLLNL.S * Jamie_Ashy_SPB_92.32989.32989.2 1.5229 0.0404 64.9 1718.3121 1717.0183 4613.6 275 2.788 35.7 1 R.ISNLNNFIKPSDIIK.L Reverse_YCR030C 2 2 2.5% 870 96137 8.6 U SYP1 SGDID:S000000626, Chr III from 176433-173821, reverse complement, Verified ORF, "Protein with a potential role in actin cytoskeletal organization; overexpression suppresses a pfy1 (profilin) null mutation" * Jamie_Ashy_SPB_107.38460.38460.1 1.4255 0.2129 50.0 1059.66 1059.2565 4979.2 13 4.543 50.0 1 R.SPPLAPARIH.L * Jamie_Ashy_SPB_3.01112.01112.3 1.9771 0.0845 56.9 1298.6344 1300.4111 5363.1 97 3.374 40.9 1 K.QYISSTATSSVR.T YPL058C 2 2 2.4% 1511 171064 6.8 U PDR12 SGDID:S000005979, Chr XVI from 450374-445839, reverse complement, Verified ORF, "Plasma membrane weak-acid-inducible ATP-binding cassette (ABC) transporter, required for weak organic acid resistance, strongly induced by sorbate and benzoate, regulated by War1p, mutants exhibit sorbate hypersensitivity" * Jamie_Ashy_SPB_45.15924.15924.2 1.6885 0.1517 21.3 1446.0521 1448.5725 5749.8 79 3.857 38.5 1 L.EQGIEPGDSGIAFK.N * Jamie_Ashy_SPB_100.36003.36003.4 3.0537 0.1099 16.7 2653.2166 2655.155 4493.0 14 3.773 27.3 1 A.SMINSNLFAAMLLFCGILQPREK.M YPR189W 2 2 2.4% 1432 163725 5.4 U SKI3 SGDID:S000006393, Chr XVI from 912660-916958, Verified ORF, "Protein involved in exosome mediated 3' to 5' mRNA degradation and translation inhibition of non-poly(A) mRNAs; forms complex with Ski2p and Ski8p; required for repressing propagation of dsRNA viruses" * Jamie_Ashy_SPB_77.27746.27746.2 1.576 0.0954 11.7 2386.2922 2384.7349 6735.8 8 3.773 30.0 1 L.DLYSQGFLLKSVSIAKDTIER.I * Jamie_Ashy_SPB_24.08538.08538.2 1.9133 0.0827 15.8 1624.2322 1621.7875 6218.3 5 3.071 50.0 1 K.DTEFAQQVLNKLQS.L YAL017W 2 2 2.4% 1356 152330 5.7 U PSK1 SGDID:S000000015, Chr I from 120226-124296, Verified ORF, "One of two (see also PSK2) PAS domain containing S/T protein kinases; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status" * Jamie_Ashy_SPB_19.06585.06585.2 1.5647 0.1092 13.1 2082.632 2080.3054 6518.5 142 3.466 28.9 1 K.EKHAITHKGTSSSVASLQTP.P * Jamie_Ashy_SPB_118.42268.42268.2 1.6444 0.0817 13.1 1393.9321 1393.6659 7767.6 432 3.015 40.9 1 K.KILLDSLAEPFF.E YMR304W 2 2 2.4% 1230 143563 5.0 U UBP15 SGDID:S000004920, Chr XIII from 874986-878678, Verified ORF, "Ubiquitin-specific protease that may play a role in ubiquitin precursor processing" * Jamie_Ashy_SPB_11.03919.03919.2 2.0968 0.1221 32.4 1258.4922 1257.5162 3546.1 86 3.923 55.0 1 S.VNITLQEALKK.E * Jamie_Ashy_SPB_2.00682.00682.3 2.5579 0.119 81.9 2235.9243 2236.6218 4184.4 243 3.636 23.6 1 K.YAHVKPEYLKIIALYSNGR.F YKL215C 2 2 2.4% 1286 140427 6.8 U YKL215C SGDID:S000001698, Chr XI from 30688-26828, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * Jamie_Ashy_SPB_33.11847.11847.2 1.8592 0.1099 16.1 2108.7322 2110.3774 7193.7 395 3.513 25.0 1 R.AHHADIGGILPGSVPPNSKEL.Y * Jamie_Ashy_SPB_19.06652.06652.2 1.6189 0.2971 80.4 939.9922 940.9878 3956.5 1 4.449 72.2 1 K.TPGGGGFGQY.K Reverse_YJL092W 2 2 2.4% 1174 134306 8.8 U HPR5 SGDID:S000003628, Chr X from 257339-260863, Verified ORF, "DNA helicase and DNA-dependent ATPase involved in DNA repair, required for proper timing of commitment to meiotic recombination and the transition from Meiosis I to Meiosis II; potential Cdc28p substrate" * Jamie_Ashy_SPB_5.01734.01734.3 2.9949 0.0302 17.7 1203.5944 1202.3501 5857.8 272 3.315 55.6 1 R.REISIDNLLE.E * Jamie_Ashy_SPB_56.19927.19927.2 1.876 0.106 14.4 2033.7722 2033.4802 7872.0 466 3.239 26.5 1 I.FDAIVSRGKTPIDLMIKK.S YJL154C 2 2 2.4% 944 109142 6.3 U VPS35 SGDID:S000003690, Chr X from 133853-131019, reverse complement, Verified ORF, "Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps26p and Vps29p that selects cargo proteins for endosome-to-Golgi retrieval" * Jamie_Ashy_SPB_41.14584.14584.2 1.7979 0.0737 11.4 1809.8522 1807.1002 6413.5 63 3.1 36.7 1 T.LLQTTLHLNPDVSINK.I * Jamie_Ashy_SPB_18.06168.06168.2 1.8103 6.0E-4 12.8 832.5722 830.8266 3670.6 10 3.012 83.3 1 R.QLEDDPN.A Reverse_YDR303C 2 2 2.4% 885 101720 8.0 U RSC3 SGDID:S000002711, Chr IV from 1071380-1068723, reverse complement, Verified ORF, "One of 15 subunits of the 'Remodel the Structure of Chromatin' (RSC) complex; essential gene required for regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p" * Jamie_Ashy_SPB_5.01729.01729.2 1.5553 0.1302 15.0 917.03217 918.1216 4560.6 257 3.573 64.3 1 T.KILSESIK.S * Jamie_Ashy_SPB_11.03711.03711.3 2.0831 0.1431 10.8 1542.3844 1541.8748 5517.2 85 3.681 41.7 1 T.IKKGTIEQLELLR.G YDL239C 2 2 2.4% 790 91740 8.6 U ADY3 SGDID:S000002398, Chr IV from 28775-26403, reverse complement, Verified ORF, "Protein required for spore wall formation, thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p" * Jamie_Ashy_SPB_26.09213.09213.2 1.9899 0.1613 34.3 2268.0522 2265.5427 4512.1 209 3.987 30.6 1 L.NDVYSYMQNLSLFEKDLGK.F * Jamie_Ashy_SPB_40.14169.14169.2 2.0112 0.0983 19.8 1936.3522 1937.2177 7144.8 3 3.578 50.0 1 V.YSYMQNLSLFEKDLGK.F Reverse_YPL082C 2 2 2.3% 1867 209975 6.6 U MOT1 SGDID:S000006003, Chr XVI from 404080-398477, reverse complement, Verified ORF, "Essential abundant protein involved in regulation of transcription, removes Spt15p (TBP) from DNA via its C-terminal ATPase activity, forms a complex with TBP that binds TATA DNA with high affinity but with altered specificity" * Jamie_Ashy_SPB_66.23647.23647.3 2.5097 0.0429 51.0 3541.6143 3537.1543 8316.2 17 3.374 20.8 1 K.ETGLFGPMLFDFLSWLELVNNQIPTGTLILR.H * Jamie_Ashy_SPB_29.10357.10357.2 2.175 0.1248 36.1 1405.6522 1405.5529 4836.4 8 3.716 63.6 1 K.NLFALWNVGDQQ.Y YKR031C 2 2 2.3% 1683 195203 7.6 U SPO14 SGDID:S000001739, Chr XI from 506037-500986, reverse complement, Verified ORF, "Phospholipase D, catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis" * Jamie_Ashy_SPB_108.38719.38719.2 2.0174 0.2216 100.0 1573.9122 1571.7366 5464.3 2 4.573 50.0 1 R.EKRRTPNGKEAER.K * Jamie_Ashy_SPB_104.37405.37405.2 1.1056 0.0107 60.1 3060.0522 3062.335 8681.0 6 2.931 22.9 1 R.LRKNDEHKKEVDGYGPDCWKKESNK.K Reverse_YOL004W 2 2 2.3% 1536 174838 5.6 U SIN3 SGDID:S000005364, Chr XV from 316938-321548, Verified ORF, "DNA binding subunit of Sin3p-Rpd3p histone deacetylase complex, involved in transcriptional repression of meiosis-specific genes during vegetative growth and silencing" * Jamie_Ashy_SPB_71.25545.25545.3 2.652 0.2165 30.9 2395.7644 2395.677 5393.2 32 4.161 28.9 1 K.RTFVDYSGNEIHLQYTIPQI.K * Jamie_Ashy_SPB_11.03864.03864.3 2.7052 0.0779 10.8 1519.7043 1518.6671 5870.8 4 3.563 42.9 1 M.LSANISPSSSSSKPR.P YJR092W 2 2 2.3% 1448 165023 5.0 U BUD4 SGDID:S000003852, Chr X from 598649-602995, Verified ORF, "Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding; potential Cdc28p substrate" * Jamie_Ashy_SPB_76.27228.27228.2 1.8132 0.051 13.3 2171.2722 2169.634 7244.5 374 3.835 25.0 1 F.NESSRVLIPAKIIQSLLMR.M * Jamie_Ashy_SPB_27.09703.09703.2 1.9838 0.1062 17.0 1656.9521 1659.8981 6343.6 319 3.359 35.7 1 R.EERITEPRTGLGIGM.L YKR082W 2 2 2.3% 1157 133320 5.1 U NUP133 SGDID:S000001790, Chr XI from 592467-595940, Verified ORF, "Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC), localizes to both sides of the NPC, required to establish a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p" * Jamie_Ashy_SPB_7.02247.02247.2 1.5183 0.0787 11.5 1239.2322 1241.3518 5091.6 148 4.093 50.0 1 L.RLHSHFDNSK.V * Jamie_Ashy_SPB_70.25162.25162.2 1.4582 0.1036 10.8 1875.2722 1878.179 7130.2 4 3.05 37.5 1 R.IVLLNASNEGDKLLQHI.V Reverse_YOL087C 2 2 2.3% 1116 125382 6.7 U YOL087C SGDID:S000005447, Chr XV from 158636-155286, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_28.09912.09912.2 2.0172 0.2641 35.3 1552.1522 1554.7458 7167.9 8 4.573 46.2 1 S.GSSQTRFYPKKSAP.L * Jamie_Ashy_SPB_4.01173.01173.3 2.4159 0.1584 10.7 1327.3143 1327.6067 4213.2 96 3.7 40.9 1 K.GPIDPYLIKTIP.L YOR048C 2 2 2.3% 1006 115934 6.8 U RAT1 SGDID:S000005574, Chr XV from 421650-418630, reverse complement, Verified ORF, "Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as mRNA transcription termination" * Jamie_Ashy_SPB_6.01971.01971.2 2.0978 0.0792 92.5 1354.9722 1355.6818 5348.7 56 3.433 58.3 1 R.KVLVMAVDGVAPR.A * Jamie_Ashy_SPB_36.12998.12998.2 1.6595 0.0195 69.2 1144.7522 1146.1992 5387.5 1 2.99 72.2 1 K.DEIVADVNDR.E YOR018W 2 2 2.3% 837 92349 8.8 U ROD1 SGDID:S000005544, Chr XV from 364368-366881, Verified ORF, "Membrane protein; overexpression confers resistance to the GST substrate o-dinitrobenzene as well as to zinc and calcium; contains 2 PY motifs, which are required for Rod1p interaction with Rsp5p, a hect-type ubiquitin ligase" * Jamie_Ashy_SPB_120.43086.43086.2 1.5114 0.0655 10.0 983.0522 983.06903 5777.1 48 3.361 62.5 1 M.FSSSSRPSK.E * Jamie_Ashy_SPB_4.01466.01466.2 1.8357 0.0605 17.4 1148.7322 1149.2522 6225.9 57 3.203 61.1 1 S.SNNLKRSSSR.T YJL130C 2 2 2.2% 2214 245123 5.9 U URA2 SGDID:S000003666, Chr X from 172285-165641, reverse complement, Verified ORF, "Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP" * Jamie_Ashy_SPB_13.04528.04528.2 1.6579 0.0969 12.4 2109.0522 2108.405 5735.8 168 3.289 27.5 1 P.VIVRAAYALGGLGSGFANNEK.E * Jamie_Ashy_SPB_50.18015.18015.2 1.7846 0.0556 78.2 2958.8123 2959.1902 4854.1 80 3.321 23.1 1 R.FSITEEAIADNLDAAEDAIPEQPLEQK.L Reverse_YKL182W 2 2 2.2% 2051 228689 5.9 U FAS1 SGDID:S000001665, Chr XI from 100676-106831, Verified ORF, "Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities" * Jamie_Ashy_SPB_10.03583.03583.4 2.5469 0.1512 17.9 3501.7766 3498.032 5052.1 124 3.545 17.7 1 L.GKSKLDEFAAKEMLTLAPQTFQTASLLGKESR.F * Jamie_Ashy_SPB_60.21326.21326.2 1.6922 0.0484 73.4 1749.7722 1749.9604 6720.4 119 3.332 38.5 1 R.PDFDLNFPEDIWLK.W Reverse_YFL033C 2 2 2.2% 1770 196530 6.5 U RIM15 SGDID:S000001861, Chr VI from 74425-69113, reverse complement, Verified ORF, "Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; originally identified as a regulator of IME2" * Jamie_Ashy_SPB_21.07520.07520.4 2.8958 0.077 76.8 3382.6565 3380.8284 6331.8 31 3.315 22.4 1 R.QSFSKSLVRQRSFYSSVTTTNAPSLPSKFK.T * Jamie_Ashy_SPB_33.11640.11640.2 1.6847 0.1522 27.6 1070.5721 1071.1932 3062.2 215 3.565 50.0 1 L.SYTMANDLR.L Reverse_YNL172W 2 2 2.2% 1748 196143 6.0 U APC1 SGDID:S000005116, Chr XIV from 310638-315884, Verified ORF, "Largest subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition" * Jamie_Ashy_SPB_94.33609.33609.2 1.9249 0.1213 22.4 1980.5721 1978.2096 5926.5 455 4.259 32.4 1 K.DESFILGANLKLSRGEDI.D * Jamie_Ashy_SPB_114.40977.40977.2 1.2729 0.1548 11.6 2285.9521 2287.5957 5959.7 96 3.639 20.0 1 S.LVLSFADHEMKLGGVVDDNAR.S YER013W 2 2 2.2% 1145 130010 7.8 U PRP22 SGDID:S000000815, Chr V from 178840-182277, Verified ORF, "DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes" * Jamie_Ashy_SPB_23.08148.08148.2 1.949 0.0722 16.2 1553.1721 1554.7527 4979.3 25 3.397 50.0 1 L.KLISCHSDPDLIR.K * Jamie_Ashy_SPB_12.04080.04080.2 1.6875 0.0646 12.9 1491.2522 1490.6621 5733.1 144 2.989 54.5 1 A.KDQNSWRLSSIR.Q Reverse_YML104C 2 2 2.2% 1127 129981 6.4 U MDM1 SGDID:S000004572, Chr XIII from 62322-58939, reverse complement, Verified ORF, "Intermediate filament protein, required for nuclear and mitochondrial transmission to daughter buds" * Jamie_Ashy_SPB_18.06483.06483.2 1.9878 0.0209 79.2 1530.7122 1529.78 6045.3 432 3.053 41.7 1 R.QLSTLALQETRLR.E * Jamie_Ashy_SPB_21.07447.07447.2 1.8239 0.1073 17.7 1459.1122 1459.7751 4472.0 281 3.026 50.0 1 P.KLSLSKHIKYNK.N YKL105C 2 2 2.2% 1132 125593 5.8 U YKL105C SGDID:S000001588, Chr XI from 242227-238829, reverse complement, Uncharacterized ORF, "Putative protein of unknown function" * Jamie_Ashy_SPB_47.16866.16866.2 1.5489 0.1422 13.6 1325.5521 1328.5261 5067.2 6 3.794 45.5 1 R.TTSIAGINRYTM.L * Jamie_Ashy_SPB_22.07780.07780.2 1.5358 0.085 10.6 1520.9321 1521.6238 5644.4 101 2.996 50.0 1 D.DENSFIEPSVLDR.S Reverse_YJR091C 2 2 2.2% 1091 120374 7.0 U JSN1 SGDID:S000003851, Chr X from 598250-594975, reverse complement, Verified ORF, "Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; overexpression suppresses a tub2-150 mutation and causes increased sensitivity to benomyl in wild-type cells" * Jamie_Ashy_SPB_81.29196.29196.2 1.9868 0.115 19.5 1737.3522 1739.0374 5926.8 496 3.979 28.6 1 L.ITLSALRHGCLEVIR.K * Jamie_Ashy_SPB_30.10808.10808.2 1.801 0.1026 17.9 1063.2922 1061.2444 3610.1 2 3.937 62.5 1 V.GQTVQMIQR.P YDR038C 2 2 2.2% 1091 120296 5.5 U ENA5 SGDID:S000002445, Chr IV from 530693-527418, reverse complement, Verified ORF, "Protein with similarity to P-type ATPase sodium pumps" YDR040C 2 2 2.2% 1091 120357 5.6 U ENA1 SGDID:S000002447, Chr IV from 538463-535188, reverse complement, Verified ORF, "P-type ATPase sodium pump, involved in Na+ and Li+ efflux to allow salt tolerance" YDR039C 2 2 2.2% 1091 120317 5.5 U ENA2 SGDID:S000002446, Chr IV from 534578-531303, reverse complement, Verified ORF, "P-type ATPase sodium pump, involved in Na+ efflux to allow salt tolerance; likely not involved in Li+ efflux" Jamie_Ashy_SPB_106.37994.37994.2 1.6068 0.1301 15.1 1774.9922 1773.9377 4955.6 81 3.901 34.4 1 R.NGKSETINSKDVVPGDI.C Jamie_Ashy_SPB_19.06693.06693.2 1.302 0.0528 63.9 867.2322 870.0575 2556.0 234 2.921 58.3 1 R.MIEALHR.R Reverse_YDR457W 3 3 2.1% 3268 374185 5.2 U TOM1 SGDID:S000002865, Chr IV from 1369780-1379586, Verified ORF, "E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators" * Jamie_Ashy_SPB_76.27129.27129.2 1.4981 0.0999 12.9 2572.392 2573.0227 6530.1 36 3.446 27.5 1 D.MQEKVSTQLKYEVVKKVYDQK.N * Jamie_Ashy_SPB_117.42166.42166.2 1.6875 0.0782 12.1 1753.5322 1754.9618 8726.2 22 3.386 39.3 1 R.ATECLVDIFQSPSRM.V * Jamie_Ashy_SPB_85.30492.30492.3 2.485 0.2069 10.9 3373.8843 3371.8352 8833.6 1 4.492 19.2 1 I.IALFDRAELHQPVQSFSGNGLSMGFLVSTFT.D YGR271W 2 2 2.1% 1967 224828 6.5 U SLH1 SGDID:S000003503, Chr VII from 1031797-1037700, Verified ORF, "Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses" * Jamie_Ashy_SPB_45.16072.16072.2 2.283 0.0707 19.3 2175.912 2173.512 6713.3 73 3.93 26.3 1 K.KIGKLINEAETTLSSNVLLE.T * Jamie_Ashy_SPB_45.16003.16003.4 2.5259 0.131 17.4 2531.8564 2534.0635 4471.8 74 3.474 26.7 1 T.PQQKKKKLLTLEEIGRFGYKK.L YML103C 2 2 2.1% 1655 188576 5.6 U NUP188 SGDID:S000004571, Chr XIII from 67549-62582, reverse complement, Verified ORF, "Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with Pom152p and Nic96p" * Jamie_Ashy_SPB_47.16681.16681.3 2.5996 0.1094 12.8 2190.5044 2188.4802 6385.7 68 3.802 26.3 1 K.SSILTVLQKNSLLLDSTPEE.V * Jamie_Ashy_SPB_23.08268.08268.2 2.1421 0.0905 20.1 1670.0122 1670.9896 5321.7 132 3.301 38.5 1 L.YSFKLSGKLLKEEK.I Reverse_YML059C 2 2 2.1% 1679 187132 8.1 U NTE1 SGDID:S000004524, Chr XIII from 158258-153219, reverse complement, Verified ORF, "Serine esterase that deacylates exogenous lysophospholipids, homolog of human neuropathy target esterase (NTE); mammalian NTE1 deacylates phosphatidylcholine to glycerophosphocholine" * Jamie_Ashy_SPB_38.13375.13375.4 2.3775 0.064 56.8 2350.7366 2355.6096 4946.2 19 2.648 27.8 1 R.FTITGSTINQPKHSLSSSYSAK.T * Jamie_Ashy_SPB_14.04947.04947.3 2.1156 0.0942 67.4 1585.6144 1581.8184 5821.6 427 3.734 35.4 1 R.PKKKDQSKSKNHR.K Reverse_YOR093C 2 2 2.1% 1648 186906 8.5 U YOR093C SGDID:S000005619, Chr XV from 502452-497506, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_37.13177.13177.2 1.9359 0.0993 15.1 1968.4321 1966.1094 6715.5 39 3.659 28.9 1 T.EGSLSSIDIAKTSEAGTNGK.P * Jamie_Ashy_SPB_33.11704.11704.2 1.8519 0.1046 16.5 1559.8922 1561.7324 4279.5 449 3.52 35.7 1 R.RDISNPLSTISSALS.T Reverse_YLR371W 2 2 2.1% 1356 152596 9.2 U ROM2 SGDID:S000004363, Chr XII from 862713-866783, Verified ORF, "GDP/GTP exchange protein (GEP) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEP" * Jamie_Ashy_SPB_24.08460.08460.2 1.6365 0.1271 14.8 1359.2722 1358.5798 5215.7 447 3.356 50.0 1 K.AGYYLFTIANKP.N * Jamie_Ashy_SPB_11.03849.03849.2 1.9373 0.1491 31.0 1801.4722 1802.8491 6070.7 2 4.292 37.5 1 M.ENNPIDSDDIAAEVGSR.R Reverse_YPR026W 2 2 2.1% 1211 136920 5.4 U ATH1 SGDID:S000006230, Chr XVI from 615376-619011, Verified ORF, "Acid trehalase required for utilization of extracellular trehalose" * Jamie_Ashy_SPB_37.13279.13279.2 1.756 0.0749 15.0 1299.8121 1302.2964 5924.6 4 3.245 60.0 1 R.ENESGNYFNID.Q * Jamie_Ashy_SPB_17.06001.06001.2 1.9126 0.0238 10.8 1435.2322 1434.6353 4413.8 80 3.082 46.2 1 K.LLTKIGANTFAGNN.I Reverse_YKL014C 2 2 2.0% 1764 203286 7.3 U URB1 SGDID:S000001497, Chr XI from 416556-411262, reverse complement, Verified ORF, "Nucleolar protein required for the normal accumulation of 25S and 5.8S rRNAs, associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit" * Jamie_Ashy_SPB_103.36834.36834.2 1.2638 0.1414 10.9 2562.2722 2563.0146 6137.3 447 3.82 18.2 1 K.EHLPATPSGLLDVIRSISFWILP.N * Jamie_Ashy_SPB_8.02721.02721.3 1.9784 0.1025 66.8 1270.8243 1266.3748 4055.3 36 3.841 36.4 1 K.GMPDSSADVCVK.N Reverse_YMR219W 2 2 2.0% 1658 187137 4.5 U ESC1 SGDID:S000004832, Chr XIII from 707132-712108, Verified ORF, "Protein localized to the nuclear periphery, involved in telomeric silencing; interacts with PAD4-domain of Sir4p" * Jamie_Ashy_SPB_83.29730.29730.2 1.753 0.0591 13.0 1598.8922 1595.9694 6175.3 3 3.878 43.3 1 A.VDVVKGVGKVVAGIVR.I * Jamie_Ashy_SPB_30.10710.10710.2 1.8021 0.0675 12.7 2106.612 2108.1882 7030.2 1 3.5 37.5 1 A.GDESYRQHVKHEDSEYK.E Reverse_YJR138W 2 2 2.0% 1584 181948 8.8 U IML1 SGDID:S000003899, Chr X from 684482-689236, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane" * Jamie_Ashy_SPB_33.11775.11775.2 1.4074 2.0E-4 61.6 2266.652 2265.5427 7334.7 64 2.482 30.6 1 K.VEFANKDYDNMFESLSKVK.K * Jamie_Ashy_SPB_119.42696.42696.2 1.861 0.1885 50.0 1594.5521 1591.7672 5473.1 40 4.362 41.7 1 R.QQSGFQEKKQRLN.R YJR138W 2 2 2.0% 1584 181948 8.8 U IML1 SGDID:S000003899, Chr X from 684482-689236, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane" * Jamie_Ashy_SPB_27.09518.09518.3 2.0941 0.0010 38.9 1480.2244 1482.8148 2791.4 372 3.28 36.4 1 K.APLHIVPLFRYR.D * Jamie_Ashy_SPB_83.29797.29797.2 1.4317 0.0172 62.1 2459.5322 2461.835 6928.4 97 3.221 25.0 1 R.GEDRITLVNRKWHWKKHEK.C YNL250W 2 2 2.0% 1312 152568 6.1 U RAD50 SGDID:S000005194, Chr XIV from 175411-179349, Verified ORF, "Subunit of MRX complex, with Mre11p and Xrs2p, involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining" * Jamie_Ashy_SPB_17.06120.06120.2 3.1288 0.1183 12.5 1589.6721 1588.8163 6549.1 25 3.504 58.3 1 E.KNNEMIILENQIE.K * Jamie_Ashy_SPB_67.23967.23967.2 1.6613 0.0105 64.8 1556.6721 1557.7435 6555.1 97 3.043 45.8 1 R.YTNALYNLNTIEK.D YFL008W 2 2 2.0% 1225 141280 6.5 U SMC1 SGDID:S000001886, Chr VI from 119424-123101, Verified ORF, "Subunit of the multiprotein cohesin complex, essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure" * Jamie_Ashy_SPB_11.03793.03793.2 1.9683 0.0866 15.5 1513.8522 1514.7684 5326.6 1 4.271 59.1 1 E.KRIESLQKDLQR.Q * Jamie_Ashy_SPB_6.02004.02004.2 3.9636 0.179 100.0 1475.5322 1476.546 6185.6 2 5.48 70.8 1 I.DELSNGQRSNSIR.A Reverse_YLR383W 2 2 2.0% 1114 128008 7.5 U SMC6 SGDID:S000004375, Chr XII from 885288-888632, Verified ORF, "Protein involved in structural maintenance of chromosomes; required for interchromosomal and sister chromatid recombination; homologous to S. pombe rad18" * Jamie_Ashy_SPB_44.15632.15632.2 1.8717 0.0763 15.2 1457.8722 1457.8406 6383.8 105 3.586 45.8 1 I.DKLKKVILTTGIK.R * Jamie_Ashy_SPB_7.02268.02268.2 1.9796 0.1114 32.9 919.9322 919.02563 6696.3 16 3.824 75.0 1 F.ANLSSSIAR.Q Reverse_YNR016C 3 3 1.9% 2233 250351 6.3 U ACC1 SGDID:S000005299, Chr XIV from 661376-654675, reverse complement, Verified ORF, "Acetyl-CoA carboxylase, biotin containing enzyme that catalyzes the carboxylation of acetyl-CoA to form malonyl-CoA; required for de novo biosynthesis of long-chain fatty acids" * Jamie_Ashy_SPB_4.01413.01413.2 1.9371 0.0352 12.3 1502.0322 1502.714 5173.2 228 3.412 41.7 1 P.ALSKNSLQSRLER.Y * Jamie_Ashy_SPB_94.33532.33532.3 1.9912 0.0025 33.3 2335.0745 2338.6714 4732.6 109 2.476 26.3 1 K.FTIDNAVVVFQRGRPYEPTK.V * Jamie_Ashy_SPB_27.09668.09668.2 1.2852 0.1447 12.4 1008.09216 1010.135 3192.0 166 3.694 55.6 1 L.PVPAGTQPDK.I Reverse_YJL005W 2 2 1.9% 2026 227832 7.3 U CYR1 SGDID:S000003542, Chr X from 425072-431152, Verified ORF, "Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; involved in cell cycle control and glucose and nitrogen repression of sporulation" * Jamie_Ashy_SPB_114.40863.40863.1 1.2758 0.1756 20.0 863.33 863.0446 2343.3 244 3.933 57.1 1 R.SALIKPYA.I * Jamie_Ashy_SPB_62.22356.22356.4 3.234 0.0081 65.2 3609.5764 3612.1233 9076.9 377 2.496 18.4 1 K.NLQLFSFRLATKINDDTEDGYRELQRILIK.D YLR422W 2 2 1.9% 1932 221562 7.8 U YLR422W SGDID:S000004414, Chr XII from 965893-971691, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_11.03972.03972.2 1.671 0.0864 13.5 1379.2522 1379.5963 2917.2 33 3.862 55.0 1 G.ELLSYEIDKLR.T * Jamie_Ashy_SPB_111.39712.39712.2 1.4227 0.1469 14.7 2630.612 2631.0825 6332.0 72 3.708 16.7 1 R.AVSLSVTVKEIIGKQEAEKVLSTSL.V YBL100W-B 3 3 1.9% 1770 202214 7.7 U YBL100W-B SGDID:S000002149, Chr II from 29932-31224,31226-35245, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" * Jamie_Ashy_SPB_34.12206.12206.2 1.9052 0.0262 13.6 1351.2122 1353.4686 6467.7 130 3.639 50.0 1 R.ALKEVDADISEY.N * Jamie_Ashy_SPB_80.28683.28683.2 1.4385 0.1212 11.7 2574.2722 2574.8938 5751.8 334 3.591 25.0 1 R.ALKEVDADISEYNILPSPVRSRT.P * Jamie_Ashy_SPB_99.35344.35344.2 1.9102 0.0369 12.8 1327.4521 1324.4735 4093.9 262 3.462 45.0 1 R.VNYELENIKTT.R Reverse_YER093C 2 2 1.9% 1430 164368 8.7 U TSC11 SGDID:S000000895, Chr V from 347608-343316, reverse complement, Verified ORF, "Subunit of TORC2 (Tor2p-Lst8p-Avo1-Avo2-Tsc11p-Bit61p), a membrane-associated complex that regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; involved in sphingolipid metabolism; contains a RasGEFN domain" * Jamie_Ashy_SPB_20.07198.07198.3 2.3096 0.0924 65.1 2800.8245 2800.9072 4735.8 26 3.271 21.2 1 K.SSENTITSPSSSTINKASSIQTSSNDR.S * Jamie_Ashy_SPB_39.14046.14046.2 1.6207 0.1818 26.5 1350.7922 1350.4686 6617.8 444 3.553 37.5 1 E.NTITSPSSSTINK.A YIL126W 2 2 1.9% 1359 156742 6.7 U STH1 SGDID:S000001388, Chr IX from 117992-122071, Verified ORF, "ATPase component of the ATP-dependent RSC chromatin remodeling complex required for kinetochore function in chromosome segregation; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p" * Jamie_Ashy_SPB_10.03498.03498.2 2.1304 0.1064 33.1 1443.8121 1443.5774 4430.6 155 3.443 50.0 1 L.HNQMEKDEQKR.I * Jamie_Ashy_SPB_17.05904.05904.2 2.0377 0.0594 87.2 1807.7522 1808.0416 4607.0 415 3.355 32.1 1 R.SADEKILFDKIDKER.M YBL088C 2 2 1.8% 2787 321568 6.9 U TEL1 SGDID:S000000184, Chr II from 59379-51016, reverse complement, Verified ORF, "Protein kinase, primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene" * Jamie_Ashy_SPB_28.09840.09840.2 3.3433 0.1114 16.0 1817.7322 1817.0953 8095.1 15 4.125 44.1 1 L.GSIIQLANYVQLAIANGA.P * Jamie_Ashy_SPB_44.15748.15748.3 2.0779 0.0701 45.1 3428.8145 3429.0 7140.6 288 3.234 17.7 1 K.VVPLGPKAGIIEFVANSTSLHQILSKLHTNDK.I YJR066W 3 3 1.8% 2470 281138 7.2 U TOR1 SGDID:S000003827, Chr X from 559330-566742, Verified ORF, "PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis" * Jamie_Ashy_SPB_96.34346.34346.2 1.8631 0.1313 21.4 1927.9521 1930.3439 6048.0 91 4.208 29.4 1 E.LAVPGTYFPGKPTIRIAK.F * Jamie_Ashy_SPB_67.24014.24014.2 1.5768 0.1118 12.4 1807.2522 1805.911 7189.5 272 3.777 34.4 1 K.GAITVEEAANMEAEQQN.E * Jamie_Ashy_SPB_8.02802.02802.2 1.9474 0.0481 15.6 1159.4122 1160.3158 4598.2 91 3.261 61.1 1 T.DKLTGNDIKR.F YFR019W 3 3 1.8% 2278 257447 8.3 U FAB1 SGDID:S000001915, Chr VI from 184490-191326, Verified ORF, "1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis" * Jamie_Ashy_SPB_21.07530.07530.2 2.5595 4.0E-4 17.3 1337.5922 1337.562 4860.0 3 3.308 70.0 1 T.DVYLKRAESKK.L * Jamie_Ashy_SPB_54.19284.19284.2 1.8416 0.0619 13.0 2246.0522 2247.512 7671.0 4 3.315 31.6 1 K.AVNWNSTFAIFAKSYLPSET.D * Jamie_Ashy_SPB_3.00812.00812.2 1.5358 0.1073 12.3 1317.5322 1316.4998 4061.6 22 3.622 55.0 1 E.HIFTDSDVIIR.E Reverse_YGR271W 2 2 1.8% 1967 224828 6.5 U SLH1 SGDID:S000003503, Chr VII from 1031797-1037700, Verified ORF, "Putative RNA helicase related to Ski2p, involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses" * Jamie_Ashy_SPB_104.37344.37344.3 2.3652 0.0428 49.2 2605.0144 2603.9556 5931.7 27 3.546 25.0 1 K.KQQPTMEIFGQESFTYDKIRR.L * Jamie_Ashy_SPB_18.06330.06330.2 1.8079 0.1279 10.3 1706.3121 1707.9873 6936.1 170 3.022 38.5 1 D.FYFMGIQRNVGLFD.A Reverse_YLR422W 2 2 1.8% 1932 221561 7.8 U YLR422W SGDID:S000004414, Chr XII from 965893-971691, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_68.24261.24261.2 1.7936 0.1832 30.6 1847.4722 1848.0654 6238.8 40 3.988 33.3 1 I.IESFSQYSPFRSSAIK.T * Jamie_Ashy_SPB_60.21607.21607.2 1.838 0.0418 11.5 1940.0521 1937.0758 5125.2 53 3.078 32.4 1 K.NILGGWGLDADKSSNFNQ.S YMR162C 2 2 1.8% 1656 188318 6.8 U DNF3 SGDID:S000004772, Chr XIII from 583920-578950, reverse complement, Verified ORF, "Non-essential P-type ATPase that is a potential aminophospholipid translocase, localizes to the trans-Golgi, likely involved in protein transport" * Jamie_Ashy_SPB_105.37630.37630.2 1.6445 0.1559 10.3 2104.7122 2104.4082 8570.1 1 4.312 34.2 1 S.SSPDELALVTAARDLGYIVL.N * Jamie_Ashy_SPB_35.12334.12334.2 1.5304 0.0083 64.1 1182.9521 1180.3678 4863.7 255 2.657 55.6 1 K.SLSMKNSRTR.D Reverse_YDR227W 2 2 1.8% 1358 152061 9.0 U SIR4 SGDID:S000002635, Chr IV from 917565-921641, Verified ORF, "Silent information regulator that, together with SIR2 and SIR3, is involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; potentially phosphorylated by Cdc28p; some alleles of SIR4 prolong lifespan" * Jamie_Ashy_SPB_40.14191.14191.2 1.9191 0.1053 10.4 1341.9122 1344.424 5956.7 45 3.889 50.0 1 N.SQQTGTQSHTGLV.D * Jamie_Ashy_SPB_70.25016.25016.2 1.9555 0.0696 16.7 1346.7322 1346.5236 5110.8 56 3.886 50.0 1 Q.SLSVKDTKNEPK.E YJL129C 2 2 1.8% 1235 141073 8.5 U TRK1 SGDID:S000003665, Chr X from 177224-173517, reverse complement, Verified ORF, "Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter" * Jamie_Ashy_SPB_7.02334.02334.2 1.4317 0.0302 63.9 1052.0122 1053.2213 3956.6 313 3.073 62.5 1 K.LFSGEMVNR.D * Jamie_Ashy_SPB_13.04376.04376.2 2.0235 0.0398 84.7 1607.6522 1607.013 5670.2 160 2.964 41.7 1 K.KASKKYLMKHFPK.A YLR087C 3 4 1.7% 2958 338258 5.9 U CSF1 SGDID:S000004077, Chr XII from 315732-306856, reverse complement, Verified ORF, "Protein required for fermentation at low temperature" * Jamie_Ashy_SPB_14.05019.05019.2 1.798 0.0764 15.8 1350.8722 1350.5328 3987.8 9 3.075 60.0 2 Y.QMSEKSLEKNR.T * Jamie_Ashy_SPB_54.19324.19324.2 1.7098 0.1037 15.7 1696.0122 1693.0295 7449.9 6 4.222 42.3 1 S.ELQLVMDQTKLMSR.T * Jamie_Ashy_SPB_77.27760.27760.2 1.5042 0.1799 10.6 2725.0923 2727.1877 7163.6 350 4.138 18.8 1 D.LTLLFTHPDVISMYGTGLVSDLSVF.F YBL004W 2 2 1.7% 2493 287559 7.0 U UTP20 SGDID:S000000100, Chr II from 227639-235120, Uncharacterized ORF, "Possible snoRNA-binding protein, based on computational analysis of large-scale protein-protein interaction data" * Jamie_Ashy_SPB_111.39787.39787.2 1.2514 0.1696 13.1 2604.372 2603.869 6629.4 17 3.801 21.4 1 I.SKLVADLNSYSSSRMHEYDFPR.I * Jamie_Ashy_SPB_63.22430.22430.2 1.6283 0.1069 84.4 2307.7122 2308.7764 7475.5 2 3.547 30.0 1 R.SKRAILAVNAPQISADKKLRK.H Reverse_YPL085W 3 3 1.7% 2195 241694 5.4 U SEC16 SGDID:S000006006, Chr XVI from 387064-393651, Verified ORF, "COPII vesicle coat protein required for ER transport vesicle budding and autophagosome formation; Sec16p is bound to the periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p" * Jamie_Ashy_SPB_37.13273.13273.2 1.3742 0.0375 63.6 1405.9521 1404.6708 6184.1 246 2.753 45.0 1 K.KMNVNKKEEKR.N * Jamie_Ashy_SPB_15.05286.05286.2 1.9839 0.0584 14.6 1572.0521 1571.9463 6817.3 435 2.986 37.5 1 F.VQFILLLFHALEK.Q * Jamie_Ashy_SPB_2.00629.00629.3 2.2928 0.1191 10.6 1580.9644 1583.6667 5557.1 264 4.205 32.7 1 R.SSLHAATEEYEQSM.A Reverse_YIL149C 2 2 1.7% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * Jamie_Ashy_SPB_33.11734.11734.2 1.9886 0.0566 15.6 1565.0122 1566.708 4924.4 11 3.423 50.0 1 K.QLTKDQKGEYNEL.K * Jamie_Ashy_SPB_108.38900.38900.2 1.8761 0.148 28.1 1837.9122 1836.0146 4813.2 106 3.605 32.1 1 R.YRSREENLQDKLGNL.E YMR128W 2 2 1.7% 1267 144954 6.3 U ECM16 SGDID:S000004735, Chr XIII from 523695-527498, Verified ORF, "Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis" * Jamie_Ashy_SPB_5.01635.01635.3 2.1096 0.0626 55.7 1369.3444 1369.5144 5072.6 329 3.253 34.1 1 R.FDSTAKEDTKVK.F * Jamie_Ashy_SPB_8.02857.02857.2 1.7378 0.0376 78.5 1052.1322 1052.2769 4576.9 1 3.033 68.8 1 K.FMTDGVLLR.E YGL206C 2 2 1.6% 1653 187233 5.2 U CHC1 SGDID:S000003174, Chr VII from 107508-102547, reverse complement, Verified ORF, "Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport and endocytosis; two heavy chains form the clathrin triskelion structural component; the light chain (CLC1) is thought to regulate function" * Jamie_Ashy_SPB_15.05229.05229.2 1.6393 0.1564 18.6 1959.3722 1962.121 4562.7 1 3.89 38.9 1 R.ETKDGTNSVAIVDLAKGNE.V * Jamie_Ashy_SPB_3.01118.01118.2 1.8993 0.0258 15.7 870.9922 872.95 2940.6 156 2.867 66.7 1 K.ETTDIFF.P YIL030C 2 2 1.6% 1319 151454 6.3 U SSM4 SGDID:S000001292, Chr IX from 300008-296049, reverse complement, Verified ORF, "Ubiquitin-protein ligase of the ER/nuclear envelope, required for degradation of Alpha2p and other proteins containing a Deg1 degradation signal; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation" * Jamie_Ashy_SPB_6.01868.01868.2 2.02 0.1333 32.9 1279.7722 1280.5588 4398.2 50 3.441 55.0 1 V.FHKIGPRLSPK.D * Jamie_Ashy_SPB_113.40438.40438.1 1.0788 0.0074 74.5 1161.07 1162.2657 5430.6 57 3.371 38.9 1 R.ENGMSNPTRR.L YDR150W 2 2 1.5% 2748 313032 5.4 U NUM1 SGDID:S000002557, Chr IV from 755623-763869, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" * Jamie_Ashy_SPB_116.41776.41776.4 3.1601 0.1209 12.1 3087.3364 3089.5398 4610.1 13 3.975 25.4 1 S.IDFLKEKCEKYQMLIISKHDYEEK.Q * Jamie_Ashy_SPB_46.16335.16335.2 1.5708 0.0084 63.6 1861.3322 1863.9388 6542.0 10 3.311 40.6 1 R.QQSLNSNSTSKTTHSSR.N Reverse_YMR207C 2 2 1.5% 2123 241784 8.0 U HFA1 SGDID:S000004820, Chr XIII from 683563-677192, reverse complement, Verified ORF, "Mitochondrial acetyl-coenzyme A carboxylase, catalyzes the production of malonyl-CoA in mitochondrial fatty acid biosynthesis" * Jamie_Ashy_SPB_35.12396.12396.3 2.8419 0.0745 78.3 2419.4944 2418.8333 8050.2 45 3.328 28.9 1 K.KKLEDILLSIEFEEINKDIK.K * Jamie_Ashy_SPB_16.05454.05454.2 2.4064 0.0382 17.6 1367.8922 1367.5509 3674.0 3 3.32 63.6 1 L.NGLGENLQRPLR.N YDL171C 2 2 1.5% 2145 238100 6.6 U GLT1 SGDID:S000002330, Chr IV from 155641-149204, reverse complement, Verified ORF, "NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source" * Jamie_Ashy_SPB_45.15969.15969.2 1.7295 0.1413 10.5 1846.6721 1847.0873 5384.0 141 3.617 36.7 1 L.RRNVVVQTDGQLRTGF.D * Jamie_Ashy_SPB_106.37939.37939.2 1.4782 0.0010 61.4 1746.5521 1747.0507 4246.6 14 2.92 36.7 1 R.FGVRNSGATIVVERIK.G YGL006W 2 2 1.5% 1173 130861 7.4 U PMC1 SGDID:S000002974, Chr VII from 485925-489446, Verified ORF, "Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium" * Jamie_Ashy_SPB_22.07869.07869.2 2.0111 0.0126 12.0 1231.5922 1230.4069 4922.3 70 3.367 55.0 1 D.EIKNLASDALR.A * Jamie_Ashy_SPB_22.07870.07870.2 1.4558 0.1097 13.2 997.2922 995.2131 4121.6 41 3.392 62.5 1 A.LRAISVAHK.D Reverse_YBL088C 2 2 1.4% 2787 321567 6.9 U TEL1 SGDID:S000000184, Chr II from 59379-51016, reverse complement, Verified ORF, "Protein kinase, primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene" * Jamie_Ashy_SPB_46.16352.16352.2 1.955 0.0449 12.5 2069.0723 2071.1704 6378.0 25 3.333 34.4 1 K.LARYSEQNENRDNNSIF.K * Jamie_Ashy_SPB_56.19993.19993.2 1.6766 0.1818 29.3 2649.0723 2649.9778 8239.4 15 4.186 26.2 1 W.VSRGGRDRLRFDPLAFWSNKDR.S YER172C 2 2 1.4% 2163 246183 5.5 U BRR2 SGDID:S000000974, Chr V from 536016-529525, reverse complement, Verified ORF, "RNA-dependent ATPase RNA helicase involved in the facilitation and disruption of snRNA interactions, required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis" * Jamie_Ashy_SPB_31.10960.10960.2 1.5615 0.0559 71.5 1478.4521 1478.7806 5283.8 58 2.782 45.5 1 R.EFQRRLAFLGIK.V * Jamie_Ashy_SPB_61.21909.21909.2 1.8456 0.0624 13.2 1898.2722 1899.281 6971.8 388 3.435 29.4 1 K.VVPLINVVVDILSANGYL.N Reverse_YMR012W 2 2 1.4% 1277 145165 6.4 U CLU1 SGDID:S000004614, Chr XIII from 291133-294966, Verified ORF, "eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant" * Jamie_Ashy_SPB_15.05130.05130.2 1.5043 0.0923 12.4 1184.7722 1185.3683 6763.3 282 3.363 44.4 1 L.QIGFKYSVSR.I * Jamie_Ashy_SPB_8.02726.02726.2 1.6076 0.0848 14.8 1078.9521 1078.2804 4122.1 251 3.049 64.3 1 Y.RVEHMHLR.N YPR117W 2 2 1.3% 2489 285902 8.3 U YPR117W SGDID:S000006321, Chr XVI from 760023-767492, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_32.11458.11458.3 2.4344 0.0659 67.3 1817.6044 1813.1912 5087.1 74 3.189 34.4 1 K.SVTILKELLGNVLTGVR.T * Jamie_Ashy_SPB_26.09261.09261.3 2.5256 0.2207 38.3 1745.2743 1742.9292 6483.1 17 4.024 38.3 1 V.PRSSTSSFFSSSPVLR.H YIL129C 2 2 1.3% 2376 269856 6.3 U TAO3 SGDID:S000001391, Chr IX from 113237-106107, reverse complement, Verified ORF, "Protein involved in cell morphogenesis and proliferation, associated with protein kinase Cbk1p; mutants activate OCH1 transcription" * Jamie_Ashy_SPB_56.20132.20132.2 1.7353 0.0858 12.2 1951.0922 1951.2458 6818.5 101 3.362 34.4 1 I.LFGTIIEAIPCCLSISR.T * Jamie_Ashy_SPB_26.09285.09285.2 1.5562 0.0231 63.1 1480.0322 1480.661 5672.6 405 2.729 41.7 1 R.DRTAITTGIEFQK.N Reverse_YHL030W 2 2 1.3% 1868 210430 6.5 U ECM29 SGDID:S000001022, Chr VIII from 40082-45688, Verified ORF, "Major component of the proteasome; tethers the proteasome core particle to the regulatory particle, and enhances the stability of the proteasome" * Jamie_Ashy_SPB_15.05094.05094.2 2.141 0.0536 17.4 1284.8322 1284.4111 6782.9 47 3.525 60.0 1 S.EGVSFLFDNQK.S * Jamie_Ashy_SPB_36.12979.12979.2 1.6128 0.0211 65.7 1450.1122 1449.7336 5958.4 398 3.098 45.8 1 R.DIGKSALLLSYLR.V Reverse_YDL171C 2 2 1.1% 2145 238100 6.6 U GLT1 SGDID:S000002330, Chr IV from 155641-149204, reverse complement, Verified ORF, "NAD(+)-dependent glutamate synthase (GOGAT), synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source" * Jamie_Ashy_SPB_22.07610.07610.2 2.1711 0.1506 47.1 1465.9521 1466.7642 4812.1 384 3.67 41.7 1 F.KKLYESTAKAKAK.A * Jamie_Ashy_SPB_8.02684.02684.3 3.0788 0.1292 30.6 1247.8744 1245.5046 4905.7 83 3.791 42.5 1 T.DVLFLDGPKLK.G Reverse_YKR054C 2 2 1.0% 4092 471351 6.3 U DYN1 SGDID:S000001762, Chr XI from 547567-535289, reverse complement, Verified ORF, "Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p" * Jamie_Ashy_SPB_89.31918.31918.3 2.7363 0.1507 12.3 2469.4143 2473.7078 7037.9 209 4.557 27.5 1 R.ENLHGFYTEYISSIICNGILE.Q * Jamie_Ashy_SPB_83.29869.29869.3 2.4375 0.106 75.5 2523.9243 2520.934 6675.9 28 3.969 25.0 1 R.VLKLGSSHEIVEYQAEKWFKK.I Reverse_YPR117W 2 2 1.0% 2489 285902 8.3 U YPR117W SGDID:S000006321, Chr XVI from 760023-767492, Uncharacterized ORF, "Hypothetical protein" * Jamie_Ashy_SPB_35.12298.12298.2 1.496 0.0087 64.1 1149.0521 1146.3323 4305.9 6 3.06 61.1 1 K.KIGEKSQISR.L * Jamie_Ashy_SPB_38.13474.13474.2 1.6012 0.1213 16.7 1842.6322 1842.0581 6960.6 22 3.173 43.3 1 G.TTFTDPFSIAPIPYDR.L Reverse_YLR106C 4 4 0.9% 4910 559315 5.0 U MDN1 SGDID:S000004096, Chr XII from 363739-349007, reverse complement, Verified ORF, "Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p), may mediate ATP-dependent remodeling of 60S subunits and subsequent export from nucleoplasm to cytoplasm" * Jamie_Ashy_SPB_43.15357.15357.2 1.6439 0.1679 23.0 1427.4722 1424.494 6776.9 83 3.304 41.7 1 K.NLDDPMGDSMDVD.E * Jamie_Ashy_SPB_6.02168.02168.2 1.6983 0.0319 12.5 1067.7322 1068.2175 5910.8 410 2.75 64.3 1 D.EYDPFLKR.F * Jamie_Ashy_SPB_55.19809.19809.2 1.7149 0.1146 15.0 1417.7122 1416.6176 5539.5 58 3.866 45.8 1 D.QDNAINLATKLSK.I * Jamie_Ashy_SPB_35.12380.12380.2 1.4375 0.0056 63.5 1057.5322 1057.2815 4524.0 18 2.505 75.0 1 K.ILDRVSVVR.T YKR054C 2 2 0.8% 4092 471351 6.3 U DYN1 SGDID:S000001762, Chr XI from 547567-535289, reverse complement, Verified ORF, "Cytoplasmic heavy chain dynein, microtubule motor protein, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p" * Jamie_Ashy_SPB_38.13406.13406.2 1.9627 0.101 10.9 1513.2722 1513.6594 5684.5 156 3.06 45.8 1 Q.AESEFSKNMLDLE.K * Jamie_Ashy_SPB_58.20768.20768.2 1.5188 0.103 12.8 2141.132 2142.5864 8100.2 281 3.371 27.8 1 R.KPISPTLTPPEALKILEFF.N Reverse_YOL081W 2 2 0.8% 3079 351669 7.1 U IRA2 SGDID:S000005441, Chr XV from 171069-180308, Verified ORF, "GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, has similarity to Ira1p and human neurofibromin" * Jamie_Ashy_SPB_32.11436.11436.2 1.7182 0.1082 87.3 2068.9521 2071.474 6324.0 2 3.857 38.2 1 K.SEDFLGILSVLLLHCLLK.T * Jamie_Ashy_SPB_15.05134.05134.2 1.6644 0.0305 12.3 799.9122 800.89844 3638.9 43 2.88 70.0 1 I.NRFAHR.N Reverse_YBR140C 2 2 0.8% 3092 350980 6.6 U IRA1 SGDID:S000000344, Chr II from 526622-517344, reverse complement, Verified ORF, "GTPase-activating protein that negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase" * Jamie_Ashy_SPB_118.42187.42187.2 1.9211 0.1546 27.4 1684.2522 1686.9268 8110.2 354 4.026 41.7 1 D.EDRKQLDMERLPR.S * Jamie_Ashy_SPB_23.08106.08106.3 1.9143 0.0165 37.3 1365.6843 1367.5883 3735.8 22 3.087 34.1 1 R.ETTIIAQVLNHK.E YDR457W 2 2 0.6% 3268 374186 5.2 U TOM1 SGDID:S000002865, Chr IV from 1369780-1379586, Verified ORF, "E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators" * Jamie_Ashy_SPB_84.30162.30162.2 1.7468 0.1859 33.3 1513.1522 1512.7863 4774.9 375 3.447 37.5 1 K.ALYVYEIILAKSE.L * Jamie_Ashy_SPB_9.03254.03254.2 1.7075 0.0289 12.5 863.7322 866.00433 5186.8 4 3.597 75.0 1 R.VENLFFP.F Proteins Peptide IDs Spectra Unfiltered 11323 90679 96014 Filtered 1052 3148 3254 Forward matches 568 2006 2110 Decoy matches 484 1142 1144 Forward FP rate 85.21 56.93 54.22 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0