DTASelect v2.0.16 /data/1/catclw/Projects/SPB/phospho2 /garibaldi/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. -p 1 --fp 0.1 --modstat --sp --noxc --nodcn --extra -e con true Use criteria 0.0 Minimum peptide confidence 0.1 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling con Exclude protein names matching 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YBR109C 11 20 48.3% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * 02142006-Holinger-04_itms_5.05114.05114.2 3.5062 0.2184 98.2 1427.6946 1428.5413 5976.2 1 4.665 75.0 2 A.FALFDKDNNGSIS.S * 02142006-Holinger-05_itms_5.04792.04792.2 3.3829 0.2266 99.4 1586.8098 1587.7269 7974.2 15 5.29 53.6 1 M.RSLGLSPSEAEVNDL.M * 02142006-Holinger-03_itms_6.04978.04978.2 3.4815 0.2202 99.4 1741.7749 1742.8549 7464.0 1 5.316 67.9 1 N.DLMNEIDVDGNHQIE.F * 02142006-Holinger-06_itms_9.06789.06789.2 3.2751 0.2488 99.2 1975.8845 1977.1096 7250.4 1 5.216 56.2 2 N.DLMNEIDVDGNHQIEFS.E * 02142006-Holinger-05_itms_7.05912.05912.2 3.4142 0.2503 100.0 1649.7589 1650.7417 6987.7 1 5.727 65.4 1 E.IDVDGNHQIEFSEF.L * 02142006-Holinger-04_itms_7.05754.05754.2 4.6568 0.3089 100.0 1509.7107 1510.5975 11281.2 1 6.108 83.3 4 K.SNDSEQELLEAFK.V * 02142006-Holinger-04_itms_7.05882.05882.2 3.2218 0.288 100.0 1308.6361 1309.4155 6024.1 1 5.439 70.0 1 N.DSEQELLEAFK.V * 02142006-Holinger-03_itms_5.04506.04506.2 3.7199 0.2998 100.0 1277.6066 1278.4198 7281.2 1 6.161 90.0 1 K.LTDAEVDDMLR.E * 02142006-Holinger-03_itms_5.04458.04458.1 2.3165 0.2982 100.0 1277.6075 1278.4198 4346.5 10 6.174 60.0 1 K.LTDAEVDDMLR.E * 02142006-Holinger-06_itms_9.07126.07126.2 4.2359 0.3065 100.0 1505.7198 1506.6678 7179.3 1 6.179 79.2 3 K.LTDAEVDDMLREV.S * 02142006-Holinger-03_itms_7.05292.05292.2 4.002 0.2421 99.4 1392.6357 1393.5084 7122.5 1 5.267 86.4 3 L.TDAEVDDMLREV.S YOR257W 8 16 47.2% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * 02142006-Holinger-04_itms_6.05513.05513.2 5.4766 0.3126 100.0 2392.2231 2393.6099 7614.4 1 5.881 50.0 5 R.SSLQSGPLNSELLEEQKQEIY.E * 02142006-Holinger-03_itms_7.05272.05272.2 3.1378 0.3366 100.0 1487.5945 1488.5708 6657.2 1 6.481 68.2 1 L.FDMNNDGFLDYH.E * 02142006-Holinger-03_itms_7.05274.05274.2 3.1871 0.2848 100.0 1336.5842 1337.388 7110.6 1 5.827 75.0 1 M.NNDGFLDYHEL.K * 02142006-Holinger-04_itms_6.05647.05647.2 3.4654 0.2133 99.4 1222.5437 1223.2842 8004.5 2 5.354 83.3 2 N.NDGFLDYHEL.K * 02142006-Holinger-04_itms_8.06438.06438.2 4.9019 0.362 100.0 1666.79 1667.7667 6495.1 1 6.859 84.6 2 R.EILDLIDEYDSEGR.H * 02142006-Holinger-04_itms_7.05793.05793.2 4.9484 0.3831 100.0 1537.741 1538.6512 6242.8 1 6.399 87.5 3 E.ILDLIDEYDSEGR.H * 02142006-Holinger-03_itms_6.05098.05098.2 2.8899 0.339 100.0 1606.7664 1607.773 7385.6 1 6.013 61.5 1 K.ELGETLTDEELRAM.I * 02142006-Holinger-03_itms_6.04887.04887.2 3.8193 0.2946 100.0 1465.6366 1466.498 7859.1 1 6.715 75.0 1 M.IEEFDLDGDGEIN.E YJR053W 17 37 28.2% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * 02142006-Holinger-04_itms_7.06107.06107.2 5.7027 0.4072 100.0 2376.1694 2377.5671 6055.3 1 8.274 65.0 9 L.TLNGLDEPETSFEELNTTLPR.F * 02142006-Holinger-03_itms_7.05471.05471.2 5.4362 0.4474 100.0 2047.9915 2049.1987 10136.2 1 7.913 67.6 3 N.GLDEPETSFEELNTTLPR.F * 02142006-Holinger-06_itms_7.05916.05916.2 3.0155 0.1979 98.0 2129.047 2130.316 3758.5 9 4.593 44.4 3 R.FQSHETLTLEENVPPLSTS.T * 02142006-Holinger-03_itms_8.06316.06316.2 4.5904 0.4443 100.0 2403.9138 2405.3755 6437.9 1 7.39 55.3 2 K.QADDDQDMEVDQDDEFLNDF.Q * 02142006-Holinger-04_itms_9.07296.07296.2 4.0272 0.1902 100.0 2808.095 2809.7983 8171.2 1 5.731 45.5 1 K.QADDDQDMEVDQDDEFLNDFQEF.Q * 02142006-Holinger-03_itms_9.06372.06372.2 4.0038 0.3883 100.0 2275.8555 2277.2446 7627.5 1 6.433 50.0 2 Q.ADDDQDMEVDQDDEFLNDF.Q * 02142006-Holinger-02_itms_8.05921.05921.2 2.7649 0.2262 97.3 1356.5614 1357.3728 7387.4 3 4.528 75.0 2 E.VDQDDEFLNDF.Q * 02142006-Holinger-05_itms_10.07276.07276.2 2.7733 0.1937 96.6 1760.7395 1761.7955 5922.0 1 4.494 61.5 1 E.VDQDDEFLNDFQEF.Q * 02142006-Holinger-02_itms_4.03404.03404.2 4.2884 0.2516 100.0 1346.6826 1347.465 7213.2 1 6.952 90.9 1 K.IDNDTQDTILAK.F * 02142006-Holinger-04_itms_7.05961.05961.2 4.0679 0.3752 100.0 1839.8221 1840.8921 7136.2 1 6.876 62.5 2 T.GFEELEGEAIDETISSN.D * 02142006-Holinger-05_itms_5.04456.04456.2 1.8439 0.2005 95.4 1185.3115 1187.3788 5183.6 10 4.355 68.2 1 K.SSSSLPLKISPA.Q * 02142006-Holinger-02_itms_4.03480.03480.2 1.966 0.1643 96.6 1175.5706 1176.2712 4811.5 215 4.509 61.1 1 K.WEGNENVLSK.F * 02142006-Holinger-05_itms_6.05348.05348.2 4.379 0.3008 100.0 1514.8048 1515.768 6543.2 2 5.956 79.2 3 K.VIGNMILDEQNLR.W * 02142006-Holinger-06_itms_10.07504.07504.2 4.3428 0.3271 100.0 2309.192 2309.5781 7325.1 1 6.297 52.4 1 S.VSEEEADPFAGIPEINLPPVGK.S * 02142006-Holinger-06_itms_10.07448.07448.2 4.1169 0.4353 100.0 2122.0806 2123.3672 7970.0 1 7.328 57.9 3 S.EEEADPFAGIPEINLPPVGK.S * 02142006-Holinger-06_itms_10.07259.07259.2 2.8329 0.0354 94.9 1447.769 1448.6616 5860.8 3 4.336 68.2 1 L.ASWFIPRDETII.S * 02142006-Holinger-05_itms_8.06268.06268.2 2.3014 0.1327 98.8 1534.8027 1535.7399 6497.7 140 4.703 50.0 1 L.ASWFIPRDETIIS.V YOR373W 28 47 23.1% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * 02142006-Holinger-03_itms_7.05318.05318.1 2.3175 0.3432 100.0 1401.6475 1402.5059 4373.8 1 6.274 53.8 1 K.QYFPGIGSGGGTNF.G * 02142006-Holinger-03_itms_7.05247.05247.2 2.5275 0.1101 96.6 1431.7926 1429.6104 6995.2 14 4.494 62.5 1 A.NKVPESDLIVSDL.V * 02142006-Holinger-03_itms_7.05520.05520.2 4.9546 0.4197 100.0 1413.7878 1414.639 4974.9 1 7.969 87.5 4 K.VPESDLIVSDLVK.D * 02142006-Holinger-06_itms_10.07519.07519.2 2.3372 0.1081 96.0 1058.6155 1059.248 6574.0 18 4.444 66.7 1 S.DLVKDLSGVL.E * 02142006-Holinger-06_itms_8.06285.06285.2 2.3434 0.0436 95.0 1114.5565 1115.231 3806.8 106 4.336 66.7 1 E.AHDVFDGILQ.K * 02142006-Holinger-02_itms_6.04862.04862.1 2.4471 0.2581 100.0 1034.5548 1035.1852 5041.4 3 5.441 62.5 1 H.DVFDGILQK.Q * 02142006-Holinger-03_itms_7.05322.05322.2 5.5873 0.2536 100.0 2618.2195 2619.7551 10013.2 1 6.862 52.3 1 K.IQEEENLANSDDTPLDTPKFNDL.F * 02142006-Holinger-03_itms_6.05231.05231.2 5.4047 0.493 100.0 2376.1765 2378.465 8021.2 1 9.122 50.0 1 Q.EEENLANSDDTPLDTPKFNDL.F * 02142006-Holinger-04_itms_6.05535.05535.2 5.352 0.4114 100.0 1989.9678 1991.1187 8128.9 1 7.187 58.8 3 E.NLANSDDTPLDTPKFNDL.F * 02142006-Holinger-03_itms_6.04733.04733.2 4.0604 0.2726 100.0 1583.7264 1584.6794 5247.0 1 6.395 76.9 1 K.LSGSPSYDSDWEKI.L * 02142006-Holinger-03_itms_5.04485.04485.2 3.0402 0.2041 94.2 1383.6093 1384.4418 5013.9 86 4.304 63.6 1 S.GSPSYDSDWEKI.L * 02142006-Holinger-05_itms_8.06351.06351.2 3.5289 0.1355 94.2 1496.6942 1497.6012 6914.4 4 4.307 70.8 4 S.GSPSYDSDWEKIL.K * 02142006-Holinger-04_itms_7.06116.06116.2 3.0052 0.1044 95.0 1168.5569 1169.2762 4958.2 3 4.317 87.5 2 S.YDSDWEKIL.K * 02142006-Holinger-06_itms_12.08895.08895.2 3.237 0.2521 99.1 2334.1475 2335.5908 4210.5 1 4.842 47.5 1 L.NLSDNEMNTLEGIPSNVVQLF.C * 02142006-Holinger-06_itms_11.08059.08059.2 4.9741 0.3604 100.0 1832.9158 1833.9928 8517.2 1 6.122 66.7 5 L.NLSNNEINGIIDFEQL.I * 02142006-Holinger-05_itms_6.05334.05334.2 3.7056 0.4029 100.0 1426.8042 1427.6438 8691.1 1 7.402 70.8 1 E.VLDLSNNNIIGVR.N * 02142006-Holinger-03_itms_6.04986.04986.2 2.2653 0.1739 99.2 1139.645 1140.325 4268.8 54 5.25 60.0 1 K.VLNLNGNPLVS.I * 02142006-Holinger-06_itms_10.07609.07609.2 4.2002 0.3639 100.0 1784.9915 1784.063 7751.8 1 6.471 68.8 3 K.VLNLNGNPLVSIVESSK.M * 02142006-Holinger-04_itms_6.05401.05401.2 3.0437 0.352 100.0 1229.675 1230.4038 6375.8 1 6.103 77.3 1 N.GNPLVSIVESSK.M * 02142006-Holinger-06_itms_9.06852.06852.2 3.1832 0.2684 99.2 1678.7814 1679.826 4499.4 3 5.138 66.7 2 L.QNYKLDDQFTFPY.Q * 02142006-Holinger-06_itms_9.06944.06944.2 3.571 0.3157 100.0 1550.7201 1551.6953 3445.4 1 6.254 77.3 1 Q.NYKLDDQFTFPY.Q * 02142006-Holinger-04_itms_7.05819.05819.2 4.8187 0.1793 97.1 1628.8031 1629.8094 5203.0 2 4.523 70.8 4 K.LDDQFTFPYQNLK.I * 02142006-Holinger-05_itms_6.05044.05044.2 3.2204 0.2053 97.5 1004.5797 1005.2022 5503.3 1 4.54 93.8 1 K.ILKLDGFAQ.L * 02142006-Holinger-06_itms_9.07009.07009.2 3.2112 0.2522 99.4 1117.6686 1118.3617 6584.4 1 5.359 83.3 1 K.ILKLDGFAQL.S * 02142006-Holinger-04_itms_7.06246.06246.2 2.0693 0.1418 90.3 1056.5376 1057.1913 5029.5 22 4.124 75.0 1 R.DFTHLPVDL.S * 02142006-Holinger-04_itms_7.06255.06255.1 2.1654 0.2142 100.0 1056.5398 1057.1913 3602.9 7 5.001 68.8 1 R.DFTHLPVDL.S * 02142006-Holinger-06_itms_7.05712.05712.2 2.8865 0.2682 99.4 1271.6724 1272.4435 7371.5 1 5.353 75.0 1 R.DFTHLPVDLSK.E * 02142006-Holinger-06_itms_10.07485.07485.2 2.9627 0.074 95.0 1203.6682 1204.4087 4972.3 2 4.329 83.3 1 L.SKELPFLQEL.H YKL042W 8 13 22.9% 363 42271 7.9 U SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane" * 02142006-Holinger-06_itms_7.05718.05718.2 3.5549 0.3009 100.0 1796.8202 1797.9182 2556.2 2 6.156 73.1 3 K.SSRLYDDYYNIPYQ.Y * 02142006-Holinger-03_itms_8.05834.05834.2 3.1991 0.3353 100.0 2256.992 2258.4634 4900.9 5 5.436 41.2 1 R.LYDDYYNIPYQYSNPTPM.N * 02142006-Holinger-06_itms_8.06178.06178.2 5.1191 0.414 100.0 2527.1357 2528.7546 4054.5 1 7.718 52.6 3 R.LYDDYYNIPYQYSNPTPMNR.D * 02142006-Holinger-03_itms_5.04527.04527.2 5.3281 0.4396 100.0 2078.9302 2080.2275 8497.4 1 7.332 70.6 1 K.VKDPMVDDDPVSENYDQI.N * 02142006-Holinger-03_itms_6.04823.04823.2 2.833 0.2668 99.3 1728.7916 1729.859 4473.0 1 5.035 66.7 1 R.SEDGNNDRMSPLPSPL.N * 02142006-Holinger-03_itms_6.04779.04779.2 2.6763 0.1045 93.7 1642.8812 1642.7809 6917.4 31 4.261 42.9 1 S.EDGNNDRMSPLPSPL.N * 02142006-Holinger-03_itms_5.04582.04582.2 2.4637 0.1464 99.1 1526.6904 1527.7474 6544.2 1 4.861 66.7 1 T.VKVNPSDDDIMMY.E * 02142006-Holinger-03_itms_8.05939.05939.2 3.5575 0.2072 99.2 1517.7692 1518.7252 7630.1 1 5.13 75.0 2 K.LSLNNQLQELQSM.M YPL079W 3 4 19.4% 160 18274 10.4 U RPL21B SGDID:S000006000, Chr XVI from 406633-406643,407065-407536, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl21Ap and has similarity to rat L21 ribosomal protein" 02142006-Holinger-04_itms_6.05483.05483.2 2.2287 0.3342 99.4 1021.5591 1022.1864 5085.0 1 5.292 81.2 1 I.YKVGDIVDI.K 02142006-Holinger-06_itms_6.05425.05425.2 3.7288 0.3496 100.0 2171.1504 2172.4429 5939.4 1 6.359 50.0 1 S.RIVSTEGNVPQTLAPVPYET.F 02142006-Holinger-02_itms_9.06702.06702.2 3.9086 0.3673 100.0 2275.1912 2276.5916 6518.5 2 7.111 47.5 2 R.IVSTEGNVPQTLAPVPYETFI.- YIL149C 42 64 17.8% 1679 195140 6.0 U MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length" * 02142006-Holinger-06_itms_9.06996.06996.2 4.3293 0.4245 100.0 1818.9839 1820.0984 5507.9 1 7.101 63.3 1 L.NVPFESLQGVTYPVLR.K * 02142006-Holinger-03_itms_5.04511.04511.2 2.4245 0.2819 100.0 1361.7446 1362.5687 6095.9 1 5.541 59.1 1 F.ESLQGVTYPVLR.K * 02142006-Holinger-06_itms_6.05228.05228.2 2.6623 0.1428 98.9 1145.6726 1146.375 5259.2 1 4.769 77.8 1 S.LQGVTYPVLR.K * 02142006-Holinger-04_itms_6.05167.05167.2 2.9743 0.1084 99.2 1081.5209 1082.198 2971.3 3 5.145 92.9 2 R.VKEEYDIW.Q * 02142006-Holinger-04_itms_6.05435.05435.2 3.8278 0.2149 98.7 1278.6613 1277.5397 7322.6 2 4.739 83.3 3 K.LMEMENILQR.C * 02142006-Holinger-05_itms_6.05109.05109.2 3.4139 0.4015 100.0 1206.6777 1207.4124 7900.8 1 7.256 86.4 1 T.TSAVSPTVGKLF.S * 02142006-Holinger-05_itms_6.05098.05098.1 2.0467 0.2766 100.0 1105.6301 1106.3073 4930.9 3 5.084 65.0 1 T.SAVSPTVGKLF.S * 02142006-Holinger-05_itms_6.05059.05059.2 2.9764 0.2581 100.0 1018.59546 1019.2291 6475.5 1 6.011 83.3 2 S.AVSPTVGKLF.S * 02142006-Holinger-05_itms_6.05043.05043.1 2.2149 0.2155 100.0 1018.59656 1019.2291 4567.8 2 5.245 66.7 1 S.AVSPTVGKLF.S * 02142006-Holinger-05_itms_5.04866.04866.2 1.6638 0.0573 93.6 947.55896 948.1503 3657.1 10 4.264 75.0 1 A.VSPTVGKLF.S * 02142006-Holinger-03_itms_6.04688.04688.2 3.296 0.3825 100.0 1320.7291 1321.5107 6706.7 1 7.239 81.8 1 R.STELLETVSLTK.R * 02142006-Holinger-05_itms_6.05407.05407.2 3.2686 0.189 97.5 1428.7745 1429.6133 5715.0 1 4.54 79.2 1 T.ASPLSQDELISLR.K * 02142006-Holinger-03_itms_5.04495.04495.2 3.0838 0.0749 99.3 1960.0112 1961.1333 5985.3 2 4.936 44.1 1 K.ILESSNIVNENDSQAIIT.E * 02142006-Holinger-05_itms_5.04697.04697.2 4.2044 0.2771 99.4 2245.1409 2246.4363 10278.6 1 5.291 52.6 2 K.ILESSNIVNENDSQAIITER.L * 02142006-Holinger-06_itms_9.06948.06948.2 4.7611 0.1552 98.8 2118.1226 2118.3916 7307.8 3 4.729 52.9 1 R.LVEFSNVNELQEKNVELL.N * 02142006-Holinger-06_itms_8.06492.06492.2 4.0772 0.352 100.0 2231.1682 2232.4956 5657.7 6 5.461 41.7 1 R.LVEFSNVNELQEKNVELLN.C * 02142006-Holinger-03_itms_7.05461.05461.2 2.9185 0.1323 95.1 1157.645 1158.3373 4910.3 1 4.338 88.9 1 A.KDAIIELENI.N * 02142006-Holinger-03_itms_6.04777.04777.2 4.5619 0.2339 100.0 1342.7258 1343.5199 9087.6 1 5.895 81.8 2 K.DAIIELENINAK.M * 02142006-Holinger-03_itms_6.04902.04902.2 3.2174 0.2531 99.2 1243.5872 1244.3434 7586.4 1 5.055 83.3 1 K.TTLEDFENFK.G * 02142006-Holinger-06_itms_9.07042.07042.2 4.0654 0.2241 99.2 1413.6913 1414.5548 7205.7 4 5.242 68.2 6 K.TTLEDFENFKGL.A * 02142006-Holinger-04_itms_7.06069.06069.2 3.4527 0.2727 100.0 1311.6432 1313.4497 7985.4 1 5.699 80.0 1 T.TLEDFENFKGL.A * 02142006-Holinger-03_itms_6.04822.04822.2 2.4977 0.1356 96.0 1070.5216 1071.2335 4795.9 1 4.432 81.2 1 R.MLEEAIDHL.K * 02142006-Holinger-05_itms_4.04190.04190.2 2.7838 0.2255 100.0 1579.8392 1580.7783 4003.3 2 5.382 57.7 1 K.ETASFIPTKESLTR.D * 02142006-Holinger-03_itms_5.04572.04572.2 2.5989 0.1953 97.9 1004.56494 1005.15643 5358.7 7 4.564 87.5 1 K.SLLTELSNK.E * 02142006-Holinger-02_itms_5.04248.04248.2 5.0545 0.4144 100.0 1806.891 1807.9548 6740.4 1 7.748 76.7 2 K.FLDQNSDASTLEPTLR.K * 02142006-Holinger-06_itms_8.06642.06642.2 4.5259 0.3554 100.0 1936.0017 1937.154 8123.9 1 6.134 68.8 1 Q.AYEEIISSNENALIELK.N * 02142006-Holinger-03_itms_6.05012.05012.2 4.8814 0.2428 100.0 1701.8945 1702.8993 6611.3 1 6.029 78.6 3 Y.EEIISSNENALIELK.N * 02142006-Holinger-03_itms_5.04468.04468.2 3.8129 0.2504 99.4 1443.8092 1444.6683 7295.1 3 5.317 66.7 1 E.IISSNENALIELK.N * 02142006-Holinger-03_itms_6.04968.04968.2 2.4486 0.1437 99.2 946.54834 945.1039 4210.1 7 5.118 85.7 1 K.DLSELVIR.L * 02142006-Holinger-06_itms_11.07901.07901.2 2.7662 0.1391 99.3 1057.6306 1058.2633 5290.8 10 4.937 81.2 1 K.DLSELVIRL.E * 02142006-Holinger-02_itms_5.04274.04274.2 4.1676 0.4099 100.0 1531.7979 1532.6909 6355.5 1 7.196 87.5 1 R.ELISNIEQTESLR.V * 02142006-Holinger-04_itms_6.05244.05244.2 3.9863 0.3831 100.0 1759.9163 1760.939 9148.9 1 7.565 71.4 2 R.ELISNIEQTESLRVE.N * 02142006-Holinger-04_itms_5.05066.05066.2 4.801 0.4018 100.0 1960.9916 1962.121 8194.3 1 8.083 65.6 2 R.ELISNIEQTESLRVENS.V * 02142006-Holinger-06_itms_8.06129.06129.2 3.1806 0.1379 99.2 1391.7849 1392.6354 8901.0 1 5.233 68.2 2 S.AVIIDEFKDITK.E * 02142006-Holinger-05_itms_8.06305.06305.2 3.4218 0.1388 96.6 1392.7201 1390.5304 7900.4 3 4.506 72.7 2 K.DIEKLTNEISDL.K * 02142006-Holinger-02_itms_4.03472.03472.2 3.2627 0.2902 100.0 1640.6953 1641.6422 5226.1 1 7.413 85.7 1 L.TFLDNKGSGEDAEEE.L * 02142006-Holinger-03_itms_7.05438.05438.2 2.7544 0.2711 99.1 1326.6382 1327.4363 5245.4 1 4.855 63.6 1 S.SLQSFQNPFTAS.Q * 02142006-Holinger-05_itms_6.05262.05262.2 4.5157 0.3958 100.0 2052.9917 2054.1777 6733.7 1 7.87 47.4 2 K.AAINFSNVTDLTNNSTDGAK.I * 02142006-Holinger-06_itms_8.06184.06184.2 3.3311 0.2542 98.9 2267.122 2268.4424 7938.0 1 4.814 47.6 1 K.AAINFSNVTDLTNNSTDGAKIT.E * 02142006-Holinger-03_itms_6.04681.04681.2 5.3276 0.4852 100.0 1981.9523 1983.099 7152.9 1 8.398 66.7 2 A.AINFSNVTDLTNNSTDGAK.I * 02142006-Holinger-02_itms_6.04790.04790.2 3.1874 0.2156 92.3 1351.6497 1352.4435 4073.9 1 4.2 77.3 1 A.INFSNVTDLTNN.S * 02142006-Holinger-05_itms_6.05398.05398.2 5.8968 0.455 100.0 2126.0547 2126.2847 8070.9 1 7.971 63.2 3 A.INFSNVTDLTNNSTDGAKIT.E YDR356W 22 61 17.2% 944 111782 7.1 U SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner" * 02142006-Holinger-02_itms_8.05923.05923.1 2.2032 0.3249 100.0 1041.4735 1042.1945 5252.1 1 5.499 75.0 1 K.NMEFTPVGF.I * 02142006-Holinger-05_itms_8.06143.06143.1 2.7173 0.1808 100.0 1282.6532 1283.528 3362.6 1 5.226 70.0 1 K.NMEFTPVGFIK.S * 02142006-Holinger-06_itms_9.06710.06710.2 3.6273 0.3551 100.0 1282.6552 1283.528 6410.8 7 5.697 75.0 2 K.NMEFTPVGFIK.S * 02142006-Holinger-05_itms_8.06187.06187.2 2.541 0.0547 92.8 1168.6082 1169.4242 5264.5 1 4.223 83.3 1 N.MEFTPVGFIK.S * 02142006-Holinger-05_itms_7.05533.05533.2 1.8629 0.2039 96.5 1037.5674 1038.2316 3652.6 67 4.482 75.0 1 M.EFTPVGFIK.S * 02142006-Holinger-03_itms_7.05499.05499.2 3.4415 0.3083 100.0 1382.6849 1383.4979 6633.9 1 6.051 81.8 3 R.SIDDTIDSTRLF.S * 02142006-Holinger-05_itms_8.06583.06583.2 2.3549 0.1019 95.5 1599.7809 1600.6788 4252.4 2 4.377 53.8 1 F.SEASQFDDSFPEIK.A * 02142006-Holinger-03_itms_5.04615.04615.2 3.4418 0.377 100.0 1312.609 1313.4064 4748.7 1 6.504 85.0 1 A.SQFDDSFPEIK.A * 02142006-Holinger-03_itms_6.04866.04866.2 4.1919 0.3398 100.0 1383.6453 1384.4851 4635.5 1 6.629 81.8 3 A.SQFDDSFPEIKA.N * 02142006-Holinger-03_itms_5.04624.04624.2 5.8811 0.3459 100.0 2141.9443 2143.1792 9145.9 1 7.767 67.6 1 K.NDTLNNYDTLEEETDDLK.N * 02142006-Holinger-04_itms_5.04623.04623.2 5.018 0.2929 100.0 1830.9252 1831.9744 10509.2 1 6.998 66.7 4 K.TVKDQVLELENNSDVQ.S * 02142006-Holinger-05_itms_7.05590.05590.2 4.8827 0.3668 100.0 2032.0521 2032.212 8449.5 1 6.687 70.6 2 K.TVKDQVLELENNSDVQSL.K * 02142006-Holinger-05_itms_7.05683.05683.2 3.2676 0.1697 99.3 1418.6978 1419.5927 7796.7 1 4.922 77.3 2 K.NLMNELNELKSN.A * 02142006-Holinger-05_itms_9.06679.06679.2 2.6509 0.1153 94.2 1317.7468 1318.5559 6538.9 1 4.31 70.0 1 K.VKQLENDLFVI.K * 02142006-Holinger-03_itms_7.05389.05389.2 2.3419 0.2221 93.2 1218.6769 1219.4233 3958.5 5 4.246 77.8 1 K.QLENDLFVIK.K * 02142006-Holinger-06_itms_20.13380.13380.2 3.8323 0.1947 99.3 1620.8085 1621.7386 5597.8 1 4.998 73.1 23 Y.KESAKDSEDKIEEL.K * 02142006-Holinger-04_itms_5.05061.05061.2 3.6901 0.2769 100.0 1388.7327 1389.5455 6367.2 1 5.497 81.8 1 Q.NLKLQEDEISSL.K * 02142006-Holinger-06_itms_4.04208.04208.2 2.9225 0.1828 99.3 1317.7089 1318.4697 5871.7 3 4.945 75.0 1 L.SLENDRLLTEK.E * 02142006-Holinger-05_itms_7.05821.05821.2 2.4607 0.1621 92.9 1190.6875 1191.413 5407.9 59 4.231 66.7 1 K.VINDYLNKVL.A * 02142006-Holinger-04_itms_5.05079.05079.2 4.6474 0.424 100.0 2043.8903 2045.0802 6420.0 1 7.47 53.1 5 L.NISLPDDDELDRDYYNS.H * 02142006-Holinger-03_itms_5.04648.04648.2 3.904 0.2845 100.0 1842.8083 1843.8982 6747.0 1 5.831 64.3 1 N.ISLPDDDELDRDYYN.S * 02142006-Holinger-04_itms_5.05027.05027.2 4.3258 0.3324 100.0 1929.8419 1930.9763 7583.7 1 6.315 56.7 4 N.ISLPDDDELDRDYYNS.H YOR234C 1 1 16.8% 107 12168 11.1 U RPL33B SGDID:S000005760, Chr XV from 779405-779387,778859-778555, reverse complement, Verified ORF, "Ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Ap and has similarity to rat L35a; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable" YPL143W 1 1 16.8% 107 12154 11.1 U RPL33A SGDID:S000006064, Chr XVI from 282121-282139,282665-282969, Verified ORF, "N-terminally acetylated ribosomal protein L37 of the large (60S) ribosomal subunit, nearly identical to Rpl33Bp and has similarity to rat L35a; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable" 02142006-Holinger-06_itms_7.05904.05904.2 3.159 0.2219 95.7 1893.0516 1894.1783 5772.7 3 4.403 44.1 1 R.VNNPNVSLIKIEGVATPQ.E YPL124W 8 13 15.0% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * 02142006-Holinger-06_itms_10.07645.07645.1 2.3167 0.1488 100.0 1004.5145 1005.1583 2172.3 1 5.499 68.8 3 R.LPPPPFSSY.G * 02142006-Holinger-02_itms_4.03465.03465.2 2.3932 0.2607 98.2 1101.5537 1102.1893 3907.1 3 4.645 81.2 1 Q.NVIDDQRLE.I * 02142006-Holinger-03_itms_5.04534.04534.2 2.7364 0.0718 98.8 1214.6403 1215.3489 6229.9 57 4.694 77.8 1 Q.NVIDDQRLEI.K * 02142006-Holinger-05_itms_6.05390.05390.2 4.308 0.3283 100.0 1634.8463 1635.8143 8353.9 1 6.591 76.9 2 L.ERIVYDQGTVIDNL.T * 02142006-Holinger-04_itms_6.05178.05178.2 3.5143 0.2612 100.0 1505.8195 1506.6989 9049.7 4 5.713 62.5 3 E.RIVYDQGTVIDNL.T * 02142006-Holinger-04_itms_5.05081.05081.3 3.8756 0.2046 100.0 1693.8845 1694.8821 7172.8 9 5.492 42.9 1 R.IVYDQGTVIDNLTSR.I * 02142006-Holinger-04_itms_5.05093.05093.2 5.1651 0.3402 100.0 1693.891 1694.8821 7477.2 1 6.626 75.0 1 R.IVYDQGTVIDNLTSR.I * 02142006-Holinger-06_itms_9.06880.06880.2 3.7133 0.4179 100.0 1908.0181 1909.1466 7329.1 1 7.014 59.4 1 R.IVYDQGTVIDNLTSRIT.R YFR028C 11 18 14.7% 551 61907 8.0 U CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit" * 02142006-Holinger-06_itms_9.06997.06997.2 4.1921 0.3946 100.0 1469.7706 1470.6653 7876.1 1 7.146 81.8 6 R.SVYLDNTIEFLR.G * 02142006-Holinger-06_itms_9.06854.06854.2 3.0856 0.2043 100.0 1382.7358 1383.5872 7092.5 3 5.651 65.0 1 S.VYLDNTIEFLR.G * 02142006-Holinger-06_itms_9.07017.07017.2 2.4183 0.2147 99.2 963.4443 964.12665 5514.4 1 5.135 92.9 1 S.FHLDFGPM.N * 02142006-Holinger-03_itms_9.06841.06841.1 1.5372 0.1942 91.9 1148.5497 1149.3496 2786.8 3 4.864 55.6 1 L.AQVDPPFMPF.R * 02142006-Holinger-03_itms_5.04411.04411.2 1.789 0.0719 91.7 993.518 994.13806 5889.3 28 4.181 78.6 1 Q.DVVYGVWR.A * 02142006-Holinger-05_itms_8.06088.06088.2 2.3491 0.2268 95.7 901.4831 902.0379 4762.7 1 4.378 85.7 1 K.GLIDLHSF.N * 02142006-Holinger-04_itms_6.05658.05658.2 2.6641 0.2476 99.3 1192.5311 1193.2578 7467.3 1 4.936 83.3 1 Y.EHVEFGDFNV.L * 02142006-Holinger-06_itms_9.07230.07230.2 3.6008 0.3653 100.0 1305.6162 1306.4174 3895.7 1 5.814 80.0 2 Y.EHVEFGDFNVL.T * 02142006-Holinger-05_itms_9.06630.06630.2 2.4607 0.2703 99.2 1395.7594 1396.6262 5624.9 1 5.053 75.0 1 G.LYPLISLEEYR.L * 02142006-Holinger-04_itms_6.05442.05442.2 4.0034 0.33 100.0 1470.7783 1471.6102 7926.1 1 6.283 70.8 1 R.VAQNNIEGELRDL.T * 02142006-Holinger-04_itms_6.05247.05247.2 3.0098 0.2156 99.2 1058.5521 1059.1643 4787.8 2 5.203 87.5 2 N.NIEGELRDL.T Reverse_YDR382W 1 1 13.6% 110 11050 4.1 U RPP2B SGDID:S000002790, Chr IV from 1239482-1239814, Verified ORF, "Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm" * 02142006-Holinger-06_itms_20.13365.13365.2 1.616 0.2047 96.8 1103.821 1105.1063 5110.8 6 4.515 46.4 1 A.DGGAAAGAAGAAASS.A YBR118W 5 8 12.7% 458 50033 9.0 U TEF2 SGDID:S000000322, Chr II from 477665-479041, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" YPR080W 5 8 12.7% 458 50033 9.0 U TEF1 SGDID:S000006284, Chr XVI from 700592-701968, Verified ORF, "Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes" 02142006-Holinger-03_itms_10.07188.07188.1 2.0023 0.3149 100.0 1102.597 1103.3062 3242.6 1 5.545 61.1 1 K.TVPFVPISGW.N 02142006-Holinger-03_itms_9.06849.06849.2 2.4727 0.1116 92.1 2047.9846 2049.3057 6087.5 151 4.196 33.3 1 K.TVPFVPISGWNGDNMIEAT.T 02142006-Holinger-05_itms_9.06646.06646.2 4.4068 0.3146 100.0 1555.84 1556.7563 7263.8 1 5.585 80.8 3 K.TLLEAIDAIEQPSR.P 02142006-Holinger-06_itms_8.06537.06537.2 3.8057 0.3578 100.0 1476.8182 1477.8022 7772.9 1 6.026 73.1 2 R.VETGVIKPGMVVTF.A 02142006-Holinger-06_itms_8.06461.06461.2 2.3148 0.2622 98.8 1283.645 1284.457 5145.6 73 4.71 55.0 1 E.AFSEYPPLGRF.A YDL229W 6 10 12.2% 613 66602 5.4 U SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; may be involved in the folding of newly-synthesized polypeptide chains; member of the heat shock protein 70 (HSP70) family; interacts with the phosphatase subunit Reg1p" * 02142006-Holinger-05_itms_7.05936.05936.2 3.0091 0.2737 100.0 1493.7684 1494.6873 5044.2 1 5.738 66.7 1 S.FVAFTPEERLIGD.A 02142006-Holinger-03_itms_5.04600.04600.2 2.4769 0.1007 91.7 1396.7723 1397.6116 7299.2 2 4.182 70.8 1 F.KVIDVDGNPVIEV.Q 02142006-Holinger-05_itms_6.05447.05447.2 3.2414 0.3397 100.0 1564.8168 1565.769 2896.6 1 5.789 69.2 2 K.AVITVPAYFNDAQR.Q 02142006-Holinger-04_itms_9.06939.06939.2 3.521 0.2949 100.0 1250.5752 1251.3379 6491.1 1 5.443 77.8 2 Q.DFDTNLLEHF.K 02142006-Holinger-06_itms_9.06694.06694.2 3.1484 0.211 99.3 1602.7339 1603.6825 5292.9 1 4.888 73.1 1 D.SLFDGEDFESSLTR.A 02142006-Holinger-04_itms_7.06254.06254.2 3.2721 0.2426 99.4 1278.6605 1279.4326 4845.4 1 5.366 85.0 3 K.ENTLLGEFDLK.N YHR021C 1 1 12.2% 82 8865 9.1 U RPS27B SGDID:S000001063, Chr VIII from 148662-148660,148109-147864, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Ap and has similarity to rat S27 ribosomal protein" YKL156W 1 1 12.2% 82 8879 9.1 U RPS27A SGDID:S000001639, Chr XI from 158619-158621,158972-159217, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Bp and has similarity to rat S27 ribosomal protein" 02142006-Holinger-05_itms_7.05536.05536.2 3.3775 0.3322 100.0 1134.6532 1135.3489 7252.7 1 6.322 88.9 1 M.VLVQDLLHPT.A YNL225C 8 19 11.9% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * 02142006-Holinger-05_itms_7.05466.05466.2 3.9275 0.2341 100.0 1307.7065 1308.5786 8266.5 1 5.966 85.0 1 R.MLENVILGYQK.K * 02142006-Holinger-04_itms_6.05496.05496.2 5.4217 0.2195 100.0 1529.8469 1530.7588 6887.6 1 5.877 87.5 5 K.IEEQTVLIENLTK.D * 02142006-Holinger-02_itms_13.08735.08735.1 2.0477 0.3032 100.0 1214.7172 1215.4753 3037.9 70 5.735 55.0 1 K.AELFEIPIGIL.F * 02142006-Holinger-03_itms_12.08628.08628.1 2.0173 0.1995 100.0 1143.6707 1144.3965 3263.9 1 5.337 66.7 1 A.ELFEIPIGIL.F * 02142006-Holinger-03_itms_6.04804.04804.2 3.5649 0.36 100.0 1580.6792 1581.6324 6264.6 1 6.64 83.3 1 L.FFDLYDSEENSSK.L * 02142006-Holinger-05_itms_7.05978.05978.2 5.7109 0.3583 100.0 1693.7788 1694.7919 9740.1 1 7.255 84.6 8 L.FFDLYDSEENSSKL.D * 02142006-Holinger-04_itms_5.05001.05001.2 2.8787 0.1624 98.0 1146.6151 1147.2706 6229.2 69 4.599 72.2 1 T.NTLSKELDNL.R * 02142006-Holinger-04_itms_6.05283.05283.2 2.4486 0.1969 94.1 1085.6604 1086.317 6816.1 141 4.29 66.7 1 Q.NTVKEIVLAV.G YLR457C 3 5 11.6% 319 37354 10.2 U NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of the mitotic apparatus containing a coiled-coil domain, essential for the G2/M transition" * 02142006-Holinger-05_itms_6.05205.05205.2 2.889 0.2591 99.3 1226.6193 1227.3623 5921.7 1 5.003 83.3 1 I.FQTIRDVFSN.D * 02142006-Holinger-04_itms_6.05634.05634.2 4.2864 0.3211 100.0 1552.7906 1553.7094 9074.6 1 6.802 73.1 3 R.ILEDLLDSSNIHPS.Y * 02142006-Holinger-03_itms_5.04232.04232.2 3.2361 0.201 98.7 1546.7319 1547.7258 4327.5 1 4.75 70.8 1 T.YNRDGNIPEMQPL.Q YDL083C 2 7 10.5% 143 15847 10.3 U RPS16B SGDID:S000002241, Chr IV from 307789-307766,307333-306926, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps16Ap and has similarity to E. coli S9 and rat S16 ribosomal proteins" YMR143W 2 7 10.5% 143 15847 10.3 U RPS16A SGDID:S000004751, Chr XIII from 551927-551950,552495-552902, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps16Bp and has similarity to E. coli S9 and rat S16 ribosomal proteins" 02142006-Holinger-06_itms_9.07001.07001.2 5.1595 0.3665 100.0 1636.9352 1637.917 8230.7 1 7.46 75.0 5 K.VNGSPITLVEPEILR.F 02142006-Holinger-06_itms_9.06856.06856.2 2.8585 0.2229 99.3 1423.821 1424.6805 7321.5 37 4.874 54.2 2 N.GSPITLVEPEILR.F YHL015W 1 1 9.9% 121 13907 9.5 U RPS20 SGDID:S000001007, Chr VIII from 75409-75774, Verified ORF, "Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; has similarity to E. coli S10 and rat S20 ribosomal proteins" * 02142006-Holinger-05_itms_7.05759.05759.2 3.1313 0.123 99.3 1387.766 1388.647 9068.0 1 4.96 81.8 1 R.YIDLEAPVQIVK.R YBL003C 1 1 9.8% 132 13989 10.7 U HTA2 SGDID:S000000099, Chr II from 235795-235397, reverse complement, Verified ORF, "One of two nearly identical (see also HTA1) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " YDR225W 1 1 9.8% 132 13989 10.7 U HTA1 SGDID:S000002633, Chr IV from 915524-915922, Verified ORF, "One of two nearly identical (see also HTA2) histone H2A subtypes; core histone required for chromatin assembly and chromosome function; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p " 02142006-Holinger-05_itms_8.06103.06103.2 2.4476 0.1743 98.0 1444.7347 1445.5693 5582.9 2 4.605 62.5 1 R.NDDELNKLLGNVT.I YML078W 1 1 9.3% 182 19919 8.7 U CPR3 SGDID:S000004543, Chr XIII from 111002-111550, Verified ORF, "Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria" * 02142006-Holinger-06_itms_9.06909.06909.2 2.6416 0.1077 95.6 1760.7507 1762.9597 5884.8 61 4.36 40.6 1 D.LTNGFGGKSIYGSKFAD.E YFR031C-A 3 4 8.7% 254 27408 11.1 U RPL2A SGDID:S000002104, Chr VI from 221406-221403,221255-220495, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl2Bp and has similarity to E. coli L2 and rat L8 ribosomal proteins" YIL018W 3 4 8.7% 254 27408 11.1 U RPL2B SGDID:S000001280, Chr IX from 316766-316769,317170-317930, Verified ORF, "Protein component of the large (60S) ribosomal subunit, identical to Rpl2Ap and has similarity to E. coli L2 and rat L8 ribosomal proteins; expression is upregulated at low temperatures" 02142006-Holinger-03_itms_9.06772.06772.2 3.1606 0.2376 99.2 1936.0818 1937.2438 7749.5 28 5.07 39.5 1 K.ASLNVGNVLPLGSVPEGTIV.S 02142006-Holinger-03_itms_9.06388.06388.2 3.1382 0.329 100.0 2023.114 2024.322 6600.2 14 6.252 37.5 1 K.ASLNVGNVLPLGSVPEGTIVS.N 02142006-Holinger-03_itms_8.06182.06182.2 4.22 0.2687 100.0 2137.1592 2138.4258 6660.6 1 6.109 45.2 2 K.ASLNVGNVLPLGSVPEGTIVSN.V YDL130W 1 1 8.5% 106 10668 4.0 U RPP1B SGDID:S000002288, Chr IV from 229906-230019,230321-230527, Verified ORF, "Ribosomal protein P1 beta, component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; accumulation is regulated by phosphorylation and interaction with the P2 stalk component" * 02142006-Holinger-05_itms_10.07428.07428.2 2.3113 0.0836 98.2 1020.8487 1022.1864 4172.4 3 4.631 68.8 1 K.DLKEILSGF.H YLL050C 1 1 8.4% 143 15901 5.2 U COF1 SGDID:S000003973, Chr XII from 40413-40400,40220-39803, reverse complement, Verified ORF, "Cofilin, promotes actin filament depolarization in a pH-dependent manner; binds both actin monomers and filaments and severs filaments , thought to be regulated by phosphorylation at SER4, ubiquitous and essential in eukaryotes" * 02142006-Holinger-05_itms_11.08312.08312.2 2.1219 0.1612 92.2 1347.594 1345.4984 4555.2 6 4.201 50.0 1 Y.EINGNEGKRSKI.V YPL255W 2 5 8.3% 385 45384 6.5 U BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p" * 02142006-Holinger-05_itms_8.06119.06119.2 3.8225 0.2721 99.2 2125.1003 2126.3708 3730.6 1 5.097 50.0 2 L.SNSQIPFIPPQEDDPLLSK.L * 02142006-Holinger-03_itms_6.04930.04930.2 4.4486 0.2676 99.2 1668.8159 1669.8285 6364.4 1 5.139 79.2 3 R.LYQEEDLKNFEIQ.T YBR031W 3 4 8.3% 362 39092 10.6 U RPL4A SGDID:S000000235, Chr II from 300166-301254, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Bp and has similarity to E. coli L4 and rat L4 ribosomal proteins" YDR012W 3 4 8.3% 362 39062 10.6 U RPL4B SGDID:S000002419, Chr IV from 471850-472938, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl4Ap and has similarity to E. coli L4 and rat L4 ribosomal proteins" 02142006-Holinger-03_itms_8.05953.05953.1 1.6706 0.1698 100.0 1067.6395 1068.2988 7112.5 1 5.027 61.1 1 K.IPEIPLVVST.D 02142006-Holinger-06_itms_10.07865.07865.2 3.9085 0.423 100.0 1881.071 1882.2047 4481.7 1 6.754 53.1 1 K.IPEIPLVVSTDLESIQK.T 02142006-Holinger-05_itms_5.04900.04900.2 2.9103 0.3194 100.0 1374.7281 1375.5645 6021.5 20 5.75 50.0 2 K.VGYTLPSHIISTS.D YHL001W 1 3 8.0% 138 15153 10.9 U RPL14B SGDID:S000000993, Chr VIII from 104272-104400,104799-105086, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Ap and has similarity to rat L14 ribosomal protein" YKL006W 1 3 8.0% 138 15167 10.9 U RPL14A SGDID:S000001489, Chr XI from 431549-431677,432076-432363, Verified ORF, "N-terminally acetylated protein component of the large (60S) ribosomal subunit, nearly identical to Rpl14Bp and has similarity to rat L14 ribosomal protein; rpl14a csh5 double null mutant exhibits synthetic slow growth" 02142006-Holinger-04_itms_7.05899.05899.2 3.802 0.341 100.0 1212.7244 1213.46 6753.4 1 5.586 80.0 3 K.LAAIVEIIDQK.K YCR031C 1 1 8.0% 137 14537 10.7 U RPS14A SGDID:S000000627, Chr III from 178215-178209,177901-177495, reverse complement, Verified ORF, "Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Bp and similar to E. coli S11 and rat S14 ribosomal proteins" YJL191W 1 1 8.0% 138 14650 10.5 U RPS14B SGDID:S000003727, Chr X from 73786-73795,74204-74610, Verified ORF, "Ribosomal protein 59 of the small subunit, required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; nearly identical to Rps14Ap and similar to E. coli S11 and rat S14 ribosomal proteins" 02142006-Holinger-03_itms_5.04419.04419.2 2.1679 0.1822 99.3 1237.5865 1238.3422 5216.8 47 4.913 50.0 1 Y.ASFNDTFVHVT.D YNR074C 2 2 7.9% 378 41335 7.4 U AIF1 SGDID:S000005357, Chr XIV from 778738-777602, reverse complement, Verified ORF, "Mitochondrial cell death effector that translocates to the nucleus in response to apoptotic stimuli, homolog of mammalian Apoptosis-Inducing Factor, putative reductase" * 02142006-Holinger-02_itms_8.06412.06412.2 2.5597 0.1446 98.9 2385.1943 2384.525 7763.4 267 4.784 26.1 1 G.TEASLVDADCLETGHAPSGVSLGP.N * 02142006-Holinger-03_itms_6.04925.04925.2 2.9313 0.2112 96.0 1782.7552 1783.8949 6730.9 1 4.436 50.0 1 L.ETGHAPSGVSLGPNAGFGQ.F YJL190C 1 1 7.7% 130 14626 9.9 U RPS22A SGDID:S000003726, Chr X from 75301-74909, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Bp and has similarity to E. coli S8 and rat S15a ribosomal proteins" YLR367W 1 1 7.7% 130 14626 9.9 U RPS22B SGDID:S000004359, Chr XII from 856441-856573,857057-857316, Verified ORF, "Protein component of the small (40S) ribosomal subunit; nearly identical to Rps22Ap and has similarity to E. coli S8 and rat S15a ribosomal proteins" 02142006-Holinger-04_itms_6.05159.05159.2 2.4906 0.09 97.7 1027.6162 1029.1381 5961.2 112 4.553 77.8 1 V.LADALNAINN.A YBL002W 1 1 7.6% 131 14237 10.1 U HTB2 SGDID:S000000098, Chr II from 236495-236890, Verified ORF, "One of two nearly identical (see HTB1) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation" YDR224C 1 1 7.6% 131 14252 10.1 U HTB1 SGDID:S000002632, Chr IV from 914706-914311, reverse complement, Verified ORF, "One of two nearly identical (see HTB2) histone H2B subtypes required for chromatin assembly and chromosome function; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation " 02142006-Holinger-04_itms_7.05858.05858.2 3.063 0.3407 100.0 1240.6011 1241.3458 7147.0 1 6.471 83.3 1 L.NSFVNDIFER.I YER074W 1 1 7.4% 135 15329 10.5 U RPS24A SGDID:S000000876, Chr V from 306319-306321,306788-307192, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein" YIL069C 1 1 7.4% 135 15329 10.5 U RPS24B SGDID:S000001331, Chr IX from 232366-232364,231954-231550, reverse complement, Verified ORF, "Protein component of the small (40S) ribosomal subunit; identical to Rps24Ap and has similarity to rat S24 ribosomal protein" 02142006-Holinger-05_itms_6.05397.05397.2 3.014 0.3255 100.0 1167.62 1168.3378 4660.1 4 5.707 72.2 1 K.QFVVDVLHPN.R YDR072C 1 1 7.0% 527 60873 7.9 U IPT1 SGDID:S000002479, Chr IV from 591341-589758, reverse complement, Verified ORF, "Inositolphosphotransferase 1, involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), which is the most abundant sphingolipid in cells, mutation confers resistance to the antifungals syringomycin E and DmAMP1 in some growth media" * 02142006-Holinger-03_itms_10.07371.07371.3 1.9379 0.0709 100.0 4133.219 4129.798 8148.5 113 5.446 13.2 1 V.ILHLTAPILTAVYLYVFQPPGTLKCFS*FALGLQNIAG.V YKL096W 1 1 6.7% 239 24268 4.7 U CWP1 SGDID:S000001579, Chr XI from 260776-261495, Verified ORF, "Cell wall mannoprotein, linked to a beta-1,3- and beta-1,6-glucan heteropolymer through a phosphodiester bond; involved in cell wall organization" * 02142006-Holinger-06_itms_9.06871.06871.2 3.0045 0.2022 92.0 1728.8153 1729.8834 9828.8 6 4.193 46.7 1 K.SSSGFYAIKDGSSYIF.S YJL158C 1 1 6.6% 227 23242 4.7 U CIS3 SGDID:S000003694, Chr X from 122865-122182, reverse complement, Verified ORF, "Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family" YKL164C 1 1 4.4% 341 34622 6.5 U PIR1 SGDID:S000001647, Chr XI from 142824-141799, reverse complement, Verified ORF, "O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle" YKL163W 1 1 4.6% 325 33004 5.7 U PIR3 SGDID:S000001646, Chr XI from 144406-145383, Verified ORF, "O-glycosylated covalently-bound cell wall protein required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway" YJL160C 1 1 5.2% 287 30215 9.0 U YJL160C SGDID:S000003696, Chr X from 118820-117957, reverse complement, Uncharacterized ORF, "Hypothetical protein" YJL159W 1 1 3.9% 387 38619 5.4 U HSP150 SGDID:S000003695, Chr X from 120444-121607, Verified ORF, "O-mannosylated heat shock protein that is secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation" 02142006-Holinger-03_itms_7.05293.05293.2 2.5504 0.1584 97.5 1635.7869 1636.804 5011.3 34 4.541 46.4 1 R.QFQFDGPPPQAGAIY.A YLR212C 4 12 6.3% 473 52628 4.7 U TUB4 SGDID:S000004202, Chr XII from 566283-564862, reverse complement, Verified ORF, "Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body" * 02142006-Holinger-04_itms_9.06967.06967.2 4.6817 0.3797 100.0 1809.8417 1810.9734 5736.7 1 7.871 73.3 3 M.MDSEPSVIADVENTFR.G * 02142006-Holinger-04_itms_9.06959.06959.2 4.6624 0.4369 100.0 1678.8011 1679.7808 7267.0 1 7.484 71.4 4 M.DSEPSVIADVENTFR.G * 02142006-Holinger-04_itms_8.06714.06714.2 4.6692 0.5032 100.0 1563.7734 1564.6921 5125.6 1 8.71 84.6 4 D.SEPSVIADVENTFR.G * 02142006-Holinger-03_itms_6.04707.04707.2 3.4098 0.2486 100.0 1632.7421 1630.7051 7770.5 1 5.439 65.4 1 K.IDKEIDSTDNFEGF.Q YPL229W 1 1 6.3% 206 23260 6.3 U YPL229W SGDID:S000006150, Chr XVI from 117067-117687, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-02_itms_4.03498.03498.1 1.572 0.1354 100.0 1205.6075 1206.2695 2019.1 151 5.068 41.7 1 N.PGSMSSSPPNSAS.S YOR096W 1 1 6.3% 190 21622 9.8 U RPS7A SGDID:S000005622, Chr XV from 505794-505937,506339-506767, Verified ORF, "Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with Kti11p; deletion causes hypersensitivity to zymocin; has similarity to rat S7 and Xenopus S8 ribosomal proteins" * 02142006-Holinger-04_itms_10.07759.07759.1 2.5786 0.2242 100.0 1197.7306 1198.4912 5913.6 72 5.112 54.5 1 K.ALAIFVPVPSLA.G YGL147C 1 1 6.3% 191 21569 9.7 U RPL9A SGDID:S000003115, Chr VII from 228334-227759, reverse complement, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Bp and has similarity to E. coli L6 and rat L9 ribosomal proteins" YNL067W 1 1 6.3% 191 21657 9.7 U RPL9B SGDID:S000005011, Chr XIV from 499682-500257, Verified ORF, "Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl9Ap and has similarity to E. coli L6 and rat L9 ribosomal proteins" 02142006-Holinger-06_itms_7.06057.06057.2 2.4169 0.2103 99.3 1345.7192 1346.526 6624.8 4 5.02 63.6 1 R.NVPVRDGVTIEF.S YAL047C 3 4 6.1% 622 72105 5.1 U SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization" * 02142006-Holinger-06_itms_9.07188.07188.2 3.9917 0.3521 100.0 2205.057 2206.3306 8441.7 1 5.398 47.5 2 K.DGNAPSLGNDTDFRNSIIEGL.N * 02142006-Holinger-06_itms_8.06640.06640.2 3.4454 0.0934 95.4 1456.807 1457.6671 6793.7 2 4.352 70.8 1 R.NSIIEGLNLEINK.L * 02142006-Holinger-03_itms_6.04879.04879.2 3.1773 0.1595 99.3 1268.6519 1269.3971 6346.8 1 4.949 85.0 1 K.LNEQSHLLDSL.E YBR127C 2 2 5.4% 517 57749 5.1 U VMA2 SGDID:S000000331, Chr II from 492816-491263, reverse complement, Verified ORF, "Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm" * 02142006-Holinger-06_itms_9.06892.06892.2 3.7052 0.1612 98.4 1930.0721 1931.2395 7351.4 2 4.674 52.9 1 R.LNYNTVSGVNGPLVILEK.V * 02142006-Holinger-06_itms_6.05346.05346.2 2.6342 0.2177 95.7 1047.5905 1048.2273 4749.1 101 4.388 66.7 1 R.GQKIPIFSAS.G YLR075W 1 1 5.0% 221 25361 10.0 U RPL10 SGDID:S000004065, Chr XII from 282928-283593, Verified ORF, "Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subunits; regulates translation initiation; has similarity to rat L10 ribosomal protein and to members of the QM gene family" * 02142006-Holinger-06_itms_10.07818.07818.2 3.4056 0.3278 100.0 1246.7216 1247.4801 8199.3 1 6.051 80.0 1 R.VDIGQIIFSVR.T Reverse_YOL010W 1 1 4.6% 367 40171 8.8 U RCL1 SGDID:S000005370, Chr XV from 307938-309041, Verified ORF, "RNA terminal phosphate cyclase-like protein involved in rRNA processing at sites A0, A1, and A2; does not possess detectable RNA cyclase activity" * 02142006-Holinger-06_itms_13.09676.09676.2 2.0884 0.1742 98.0 1754.7312 1756.0176 4088.3 19 4.607 37.5 1 V.LHVEGGGLPPSGRKLTH.L Reverse_YDR410C 1 1 4.6% 239 27888 7.9 U STE14 SGDID:S000002818, Chr IV from 1293081-1292362, reverse complement, Verified ORF, "Farnesyl cysteine-carboxyl methyltransferase, mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane " * 02142006-Holinger-04_itms_19.13198.13198.2 1.5416 0.0699 94.8 1275.6882 1276.6049 6208.5 233 4.316 45.0 1 V.FIFIVLSLPNL.L YGR192C 1 1 4.5% 332 35747 7.0 U TDH3 SGDID:S000003424, Chr VII from 883815-882817, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " YJR009C 1 1 4.5% 332 35847 7.0 U TDH2 SGDID:S000003769, Chr X from 454595-453597, reverse complement, Verified ORF, "Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2, involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell-wall " 02142006-Holinger-03_itms_9.06523.06523.2 3.3464 0.3311 100.0 1550.8011 1551.8995 6946.6 1 5.396 64.3 1 K.VVITAPSSTAPMFVM.G Reverse_YHR199C 1 4 4.5% 310 34113 9.1 U YHR199C SGDID:S000001242, Chr VIII from 498422-497490, reverse complement, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * 02142006-Holinger-04_itms_10.07867.07867.2 3.5976 0.2047 96.3 1379.8428 1381.7422 4802.6 10 4.479 69.2 4 F.ISALALGTLVPVVK.S YPR125W 1 1 4.4% 454 52175 9.3 U YPR125W SGDID:S000006329, Chr XVI from 787957-789321, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-05_itms_7.05762.05762.2 2.1307 0.1657 95.3 2319.0012 2317.6042 9545.4 6 4.34 31.6 1 G.VPESRQQLIEVARLFTDDTV.L YFL039C 1 2 4.3% 375 41690 5.7 U ACT1 SGDID:S000001855, Chr VI from 54695-54686,54377-53260, reverse complement, Verified ORF, "Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions" * 02142006-Holinger-04_itms_6.05546.05546.2 3.9292 0.2553 99.3 1790.8986 1791.9554 5997.3 1 4.922 70.0 2 K.SYELPDGQVITIGNER.F YDL055C 1 1 4.2% 361 39566 6.3 U PSA1 SGDID:S000002213, Chr IV from 356759-355674, reverse complement, Verified ORF, "GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure" * 02142006-Holinger-06_itms_10.07750.07750.2 2.6954 0.2045 96.5 1725.9629 1726.0814 4849.0 5 4.493 50.0 1 Y.ILNPEVIDLIEMKPT.S YLR340W 1 1 4.2% 312 33717 4.8 U RPP0 SGDID:S000004332, Chr XII from 805887-806825, Verified ORF, "Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphorylated on serine 302" * 02142006-Holinger-04_itms_7.05972.05972.2 3.4016 0.2477 100.0 1470.7399 1471.6042 8059.7 13 5.846 62.5 1 S.SILDITDEELVSH.F YIL011W 1 1 4.1% 269 26308 4.3 U TIR3 SGDID:S000001273, Chr IX from 333724-334533, Verified ORF, "Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth" * 02142006-Holinger-01_itms_26.15889.15889.2 1.2356 0.1675 94.5 955.6212 954.9231 2839.4 25 4.312 50.0 1 S.ATASSDASSAS.E YML092C 1 1 4.0% 250 27162 5.7 U PRE8 SGDID:S000004557, Chr XIII from 86739-85987, reverse complement, Verified ORF, "20S proteasome beta-type subunit" * 02142006-Holinger-05_itms_16.10851.10851.2 2.0062 0.0172 90.9 1048.6426 1048.1815 3821.7 39 4.151 50.0 1 V.IATEKKSSSP.L YDR502C 1 1 3.9% 384 42256 5.4 U SAM2 SGDID:S000002910, Chr IV from 1454454-1453300, reverse complement, Verified ORF, "S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)" YLR180W 1 1 3.9% 382 41818 5.2 U SAM1 SGDID:S000004170, Chr XII from 515264-516412, Verified ORF, "S-adenosylmethionine synthetase, catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; one of two differentially regulated isozymes (Sam1p and Sam2p)" 02142006-Holinger-06_itms_6.05133.05133.2 3.1028 0.1482 99.2 1444.7632 1445.6182 6271.7 7 5.117 60.7 1 R.FVIGGPQGDAGLTGR.K YMR055C 1 1 3.9% 306 35028 8.6 U BUB2 SGDID:S000004659, Chr XIII from 387020-386100, reverse complement, Verified ORF, "Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage" * 02142006-Holinger-06_itms_9.06766.06766.2 2.6193 0.2127 99.2 1465.702 1466.5902 5301.7 2 5.134 59.1 1 R.QFPDFDADEIIR.L YDL014W 1 1 3.7% 327 34465 10.2 U NOP1 SGDID:S000002172, Chr IV from 427361-428344, Verified ORF, "Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin" * 02142006-Holinger-04_itms_7.05957.05957.2 3.5241 0.3415 100.0 1216.6627 1217.4075 5835.7 1 5.757 77.3 1 M.GGLDELFIAPGK.K YKL060C 1 2 3.6% 359 39621 5.8 U FBA1 SGDID:S000001543, Chr XI from 327131-326052, reverse complement, Verified ORF, "Fructose 1,6-bisphosphate aldolase, a cytosolic enzyme required for glycolysis and gluconeogenesis; catalyzes the conversion of fructose 1,6 bisphosphate into two 3-carbon products: glyceraldehyde-3-phosphate and dihydroxyacetone phosphate" * 02142006-Holinger-06_itms_8.06634.06634.2 3.1677 0.371 100.0 1399.7308 1400.5742 8094.9 3 6.427 58.3 2 K.TGVIVGEDVHNLF.T YML110C 1 1 3.6% 307 34685 6.6 U COQ5 SGDID:S000004578, Chr XIII from 50954-50031, reverse complement, Verified ORF, "2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; located in mitochondria" * 02142006-Holinger-04_itms_8.06539.06539.2 2.302 0.1443 92.8 1008.58777 1009.06055 4558.4 406 4.227 60.0 1 I.DVAGGSGDIAF.G Reverse_YBR257W 1 1 3.6% 279 32881 9.2 U POP4 SGDID:S000000461, Chr II from 728880-729719, Verified ORF, "Subunit of both RNase MRP, which cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA precursors to generate mature 5' ends; binds to the RPR1 RNA subunit in Rnase P" * 02142006-Holinger-03_itms_6.04747.04747.2 2.6355 0.1531 92.1 1192.615 1193.4288 6524.9 14 4.197 72.2 1 D.TIKNEYALKL.C YOL154W 1 1 3.6% 249 27567 5.0 U ZPS1 SGDID:S000005514, Chr XV from 34657-35406, Verified ORF, "Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH" * 02142006-Holinger-01_itms_15.09867.09867.2 1.4308 0.0695 92.9 988.50995 986.1558 2384.1 433 4.238 68.8 1 K.FSSGKSIIF.A YNR075W 1 1 3.5% 374 43867 7.5 U COS10 SGDID:S000005358, Chr XIV from 779916-781040, Verified ORF, "Protein of unknown function, member of a family of conserved, often subtelomerically-encoded proteins" * 02142006-Holinger-04_itms_5.05118.05118.2 1.0315 0.2448 99.4 1518.7579 1517.6595 1922.5 336 5.299 37.5 1 P.DMSDFFICANLSP.A YAL038W 1 3 3.4% 500 54545 7.7 U CDC19 SGDID:S000000036, Chr I from 71787-73289, Verified ORF, "Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration" * 02142006-Holinger-05_itms_7.05903.05903.2 4.29 0.4025 100.0 1724.9156 1725.9365 4389.5 1 6.885 62.5 3 K.GVNLPGTDVDLPALSEK.D YML085C 1 2 3.4% 447 49800 5.1 U TUB1 SGDID:S000004550, Chr XIII from 99400-99376,99259-97941, reverse complement, Verified ORF, "Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules" YML124C 1 2 3.4% 445 49694 5.2 U TUB3 SGDID:S000004593, Chr XIII from 23684-23660,23361-22049, reverse complement, Verified ORF, "Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p" 02142006-Holinger-06_itms_9.06755.06755.2 4.3284 0.3282 100.0 1744.9209 1745.9701 4643.7 1 6.529 75.0 2 R.AIYVDLEPNVIDEVR.N YCR012W 1 1 3.4% 416 44738 7.6 U PGK1 SGDID:S000000605, Chr III from 137743-138993, Verified ORF, "3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis" * 02142006-Holinger-06_itms_9.06688.06688.2 2.9147 0.349 100.0 1439.8271 1440.683 6336.7 1 6.092 61.5 1 K.ASAPGSVILLENLR.Y YKL218C 1 1 3.4% 326 34899 7.2 U SRY1 SGDID:S000001701, Chr XI from 18339-17359, reverse complement, Verified ORF, "3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required for survival in the presence of hydroxyaspartate" * 02142006-Holinger-05_itms_8.06446.06446.2 2.1624 0.1216 90.8 1138.5924 1139.3396 4249.9 202 4.144 55.0 1 Q.LAAEHGFALIP.P YHR172W 2 2 3.3% 823 96825 6.4 U SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * 02142006-Holinger-05_itms_5.04787.04787.2 2.4953 0.1659 99.2 1132.6415 1133.3329 6195.7 85 5.135 55.6 1 R.YTNNIPLLGK.L * 02142006-Holinger-03_itms_6.05060.05060.2 4.2184 0.3186 100.0 2090.9326 2092.2227 5322.4 2 5.451 59.4 1 R.YFNDYEPSDPETPIEFK.I YOR360C 1 1 3.2% 526 61000 6.6 U PDE2 SGDID:S000005887, Chr XV from 1014817-1013237, reverse complement, Verified ORF, "High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon" * 02142006-Holinger-02_itms_12.08257.08257.2 2.3254 0.1992 99.1 1790.9572 1793.931 4113.7 1 4.84 50.0 1 G.INKNSYGTVNGNSVPTQ.A Reverse_YHL021C 1 1 3.2% 465 53135 7.5 U YHL021C SGDID:S000001013, Chr VIII from 65856-64459, reverse complement, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * 02142006-Holinger-06_itms_9.06705.06705.2 2.0425 0.2294 95.3 1621.7864 1624.7068 2813.3 102 4.34 42.9 1 A.YHANVSTAQSANVDF.T Reverse_YDR190C 1 1 3.2% 463 50453 5.9 U RVB1 SGDID:S000002598, Chr IV from 841990-840599, reverse complement, Verified ORF, "Essential protein involved in transcription regulation; component of chromatin remodeling complexes; required for assembly and function of the INO80 complex; member of the RUVB-like protein family" * 02142006-Holinger-04_itms_8.06473.06473.2 2.3104 0.1204 94.7 1680.8379 1678.0281 5240.0 1 4.315 50.0 1 L.TRVILLRDILDPPVG.H Reverse_YDR014W 1 2 3.1% 647 74722 5.9 U RAD61 SGDID:S000002421, Chr IV from 474043-475986, Verified ORF, "Protein of unknown function; mutation confers radiation sensitivity" * 02142006-Holinger-02_itms_8.06096.06096.2 4.1156 0.0908 91.7 2304.0625 2305.7476 7330.0 1 4.183 42.1 2 H.VEMNTLLILIKILSDDDLMD.K Reverse_YFR051C 1 1 3.1% 546 60628 5.2 U RET2 SGDID:S000001947, Chr VI from 251790-250150, reverse complement, Verified ORF, "Delta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER" * 02142006-Holinger-06_itms_9.06736.06736.2 2.2896 0.2535 90.4 1646.7799 1643.8553 5623.8 210 4.13 37.5 1 H.GARQSTAMPSAVPAVEA.A Reverse_YJR002W 1 1 3.0% 593 66953 4.7 U MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, which is required for pre-18S rRNA processing, interacts with and controls the stability of Imp3p and Imp4p, essential for viability; similar to human Mpp10p" * 02142006-Holinger-05_itms_7.05716.05716.2 3.3401 0.0941 92.9 2027.9716 2029.004 9586.4 21 4.242 41.2 1 A.STKEDDMSPEEDNESLSD.N Reverse_YOR313C 1 2 3.0% 338 38591 9.6 U SPS4 SGDID:S000005840, Chr XV from 902869-901853, reverse complement, Verified ORF, "Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage" * 02142006-Holinger-03_itms_9.06410.06410.2 2.8433 0.1848 94.3 1113.7496 1112.4012 4954.9 1 4.311 72.2 2 T.QALIIRTLAI.K Reverse_YLR114C 1 1 2.9% 764 86426 4.5 U YLR114C SGDID:S000004104, Chr XII from 377239-374945, reverse complement, Uncharacterized ORF, "Mutation is syntheticly lethal with apl2 vps1 double mutant" * 02142006-Holinger-06_itms_20.13733.13733.2 1.4762 0.1504 94.1 2612.152 2614.87 6981.4 40 4.31 26.2 1 D.YLLTFYTFTEEFSHSGDPLAQF.P YBR158W 1 2 2.9% 549 62686 9.1 U AMN1 SGDID:S000000362, Chr II from 556543-558192, Verified ORF, "Protein required for daughter cell separation, multiple mitotic checkpoints, and chromosome stability; contains 12 degenerate leucine-rich repeat motifs; expression is induced by the Mitotic Exit Network (MEN)" * 02142006-Holinger-05_itms_6.05213.05213.2 3.7088 0.1759 98.9 1827.8562 1829.9293 6820.0 2 4.8 53.3 2 G.FAGCDVDDAGIWEFAR.L Reverse_YPR127W 1 1 2.9% 345 38601 6.0 U YPR127W SGDID:S000006331, Chr XVI from 790079-791116, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-03_itms_10.07042.07042.2 2.0913 0.1189 91.2 1088.6372 1086.19 4371.1 42 4.162 66.7 1 L.QALTISNNQP.R Reverse_YML041C 1 1 2.9% 280 32021 9.5 U VPS71 SGDID:S000004505, Chr XIII from 195755-194913, reverse complement, Verified ORF, "Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin; required for vacuolar protein sorting" * 02142006-Holinger-03_itms_18.11781.11781.2 1.372 0.1672 95.1 898.6111 896.90497 2827.7 132 4.338 57.1 1 N.GCNVCSSI.S YNL126W 2 2 2.8% 846 98227 7.5 U SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque" * 02142006-Holinger-04_itms_7.06162.06162.2 2.4347 0.2205 98.2 1233.6627 1234.3977 4197.0 2 4.61 72.2 1 K.SQNQFDLIRL.N * 02142006-Holinger-05_itms_8.06083.06083.2 3.3729 0.2119 99.3 1631.7946 1632.7673 8153.7 1 5.036 69.2 1 K.TLNIDELESVHNTF.L Reverse_YJR062C 1 1 2.8% 457 51897 5.2 U NTA1 SGDID:S000003823, Chr X from 554763-553390, reverse complement, Verified ORF, "Amidase, removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation" * 02142006-Holinger-04_itms_11.08396.08396.2 2.5056 0.0616 93.5 1550.7805 1551.7771 6424.2 2 4.256 75.0 1 L.FTKELVTDKIVEE.S YHR143W 1 2 2.8% 325 33417 4.5 U DSE2 SGDID:S000001186, Chr VIII from 385513-386490, Verified ORF, "Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP" * 02142006-Holinger-06_itms_6.05115.05115.2 2.2388 0.1015 95.6 876.5237 876.98236 3773.2 40 4.364 62.5 2 T.STLVPSSSV.D YMR001C 1 2 2.7% 705 81031 9.0 U CDC5 SGDID:S000004603, Chr XIII from 271136-269019, reverse complement, Verified ORF, "Polo-like kinase with similarity to Xenopus Plx1 and S. pombe Plo1p; found at bud neck, nucleus and SPBs; has multiple functions in mitosis and cytokinesis through phosphorylation of substrates; may be a Cdc28p substrate" * 02142006-Holinger-06_itms_9.06739.06739.2 3.9725 0.2952 100.0 2194.046 2194.3594 4586.9 1 6.028 52.8 2 M.SEAPNFEDIPEEQSLVNFK.D Reverse_YER068W 1 1 2.7% 587 65354 8.1 U MOT2 SGDID:S000000870, Chr V from 293048-294811, Verified ORF, "Component of the CCR4-NOT transcription regulatory complex, which represses transcription, at least in part, by inhibiting functional TBP-DNA interactions and also aids in transcription elongation; interacts with C-terminal region of Not1p" * 02142006-Holinger-05_itms_7.05792.05792.2 2.6398 0.1312 90.8 1721.8098 1721.821 6643.3 1 4.147 53.3 1 N.ETTPTTHHGLSDNLTP.L YDR385W 3 3 2.6% 842 93289 6.3 U EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" YOR133W 3 3 2.6% 842 93289 6.3 U EFT1 SGDID:S000005659, Chr XV from 575098-577626, Verified ORF, "Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin" 02142006-Holinger-06_itms_9.06912.06912.2 2.6739 0.1571 95.7 1210.5927 1211.3623 4159.6 2 4.383 83.3 1 R.LWGDSFFNPK.T 02142006-Holinger-04_itms_7.05952.05952.2 3.1977 0.2804 100.0 1282.7037 1283.4673 6302.6 1 5.766 77.3 1 K.NANDLPKLVEGL.K 02142006-Holinger-04_itms_7.05808.05808.2 2.4235 0.243 99.1 1097.6237 1098.2847 6298.1 9 4.843 72.2 1 A.NDLPKLVEGL.K Reverse_YEL041W 1 1 2.6% 495 55874 5.8 U YEL041W SGDID:S000000767, Chr V from 75944-77431, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-06_itms_6.05013.05013.2 2.3481 0.248 99.1 1351.6135 1352.3948 3501.3 1 4.863 58.3 1 Y.LNSTDEGVITESS.L YMR068W 1 1 2.6% 426 47140 9.3 U AVO2 SGDID:S000004672, Chr XIII from 406303-407583, Verified ORF, "Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth" * 02142006-Holinger-03_itms_17.11250.11250.2 1.8279 0.0579 90.5 1135.7815 1133.1167 4128.0 10 4.137 55.0 1 D.SFSSSSNSSSR.L YGR279C 1 2 2.6% 386 40173 4.8 U SCW4 SGDID:S000003511, Chr VII from 1049964-1048804, reverse complement, Verified ORF, "Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating" * 02142006-Holinger-04_itms_6.05196.05196.2 3.3013 0.1116 99.3 1159.6505 1160.3585 5344.6 6 5.031 77.8 2 L.AQLTDFPVIR.L YCR089W 1 1 2.4% 1609 166036 4.9 U FIG2 SGDID:S000000685, Chr III from 267430-272259, Verified ORF, "Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating" * 02142006-Holinger-05_itms_13.09540.09540.3 2.4394 0.2599 100.0 4363.492 4365.0146 11041.4 9 4.527 17.1 1 L.LFPALSTEMINTTVVSRKTLIISTEVCSHSKCVPTVITE.V YIL014W 1 1 2.4% 630 72410 5.6 U MNT3 SGDID:S000001276, Chr IX from 326101-327993, Verified ORF, "Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation" * 02142006-Holinger-03_itms_9.06777.06777.2 2.5602 0.1724 90.3 1692.8535 1691.879 3724.1 29 4.122 42.9 1 E.SIAKISETAKETEQR.V YBR248C 1 1 2.4% 552 61068 5.5 U HIS7 SGDID:S000000452, Chr II from 716460-714802, reverse complement, Verified ORF, "Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor" * 02142006-Holinger-06_itms_20.13738.13738.2 1.8677 0.1606 98.0 1394.709 1395.5522 7017.7 6 4.595 45.8 1 G.SVESPKSTGLNYI.D YBL045C 1 1 2.4% 457 50228 7.3 U COR1 SGDID:S000000141, Chr II from 135519-134146, reverse complement, Verified ORF, "Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain" * 02142006-Holinger-04_itms_5.04665.04665.2 1.9539 0.0614 91.3 1112.6202 1113.1693 5616.2 1 4.164 65.0 1 L.AVEGEPVNSPN.Y YNL009W 1 1 2.4% 420 47856 9.2 U IDP3 SGDID:S000004954, Chr XIV from 614822-616084, Verified ORF, "Peroxisomal NADP-dependent isocitrate dehydrogenase, catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids" * 02142006-Holinger-03_itms_19.12340.12340.2 1.2381 0.06 90.6 963.7325 963.1982 3297.7 1 4.131 61.1 1 F.PKSGGIAMAM.F YKL072W 1 1 2.3% 766 88836 8.6 U STB6 SGDID:S000001555, Chr XI from 299226-301526, Verified ORF, "Protein that binds Sin3p in a two-hybrid assay" * 02142006-Holinger-04_itms_6.05585.05585.2 3.3512 0.1842 94.2 2086.9712 2085.556 8000.1 114 4.307 41.2 1 D.IFKFKKVVKSTVQDMTGK.G YJL019W 1 1 2.3% 682 79175 5.3 U MPS3 SGDID:S000003556, Chr X from 402813-404861, Verified ORF, "Essential integral membrane protein required for spindle pole body duplication and for nuclear fusion, localizes to the spindle pole body half bridge, interacts with DnaJ-like chaperone Jem1p and with centrin homolog Cdc31p" * 02142006-Holinger-06_itms_11.08496.08496.2 3.1075 0.3291 100.0 1801.9435 1803.0221 5788.1 1 6.103 63.3 1 R.LLPSNLVNFENDINSL.T YER143W 1 1 2.3% 428 47354 5.1 U DDI1 SGDID:S000000945, Chr V from 456314-457600, Verified ORF, "DNA damage-inducible v-SNARE binding protein, contains a ubiquitin-associated (UBA) domain, may act as a negative regulator of constitutive exocytosis, may play a role in S-phase checkpoint control" * 02142006-Holinger-05_itms_6.05062.05062.2 2.1091 0.172 92.4 1073.5289 1074.2163 5172.4 192 4.205 66.7 1 L.DTDIDVLIGL.D YGL075C 1 1 2.3% 387 44585 8.3 U MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p" * 02142006-Holinger-05_itms_7.05775.05775.2 2.6962 0.1938 99.3 1013.0403 1014.2505 6488.0 38 4.917 75.0 1 K.LTESPIKLL.S Reverse_YOR239W 1 1 2.2% 628 71486 4.7 U ABP140 SGDID:S000005765, Chr XV from 784857-785687,785689-786744, Verified ORF, "Nonessential protein that binds actin filaments and localizes to actin patches and cables, has similarity to S-adenosylmethionine (AdoMet)-dependent methyltransferases" * 02142006-Holinger-01_itms_17.10882.10882.2 1.6681 0.0283 95.5 1560.9424 1562.8671 3958.5 415 4.377 42.3 1 A.SFVFIMVAIDVSHP.E YOR025W 1 2 2.2% 447 50524 9.1 U HST3 SGDID:S000005551, Chr XV from 378218-379561, Verified ORF, "Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism" * 02142006-Holinger-01_itms_17.10983.10983.2 2.0297 0.0708 94.1 1048.6483 1050.1407 8663.9 1 4.296 66.7 2 S.PPASRSGSMC.S Reverse_YHR179W 1 1 2.2% 400 45011 6.6 U OYE2 SGDID:S000001222, Chr VIII from 462502-463704, Verified ORF, "Widely conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye3p with slight differences in ligand binding and catalytic properties; may be involved in sterol metabolism" Reverse_YPL171C 1 1 2.2% 400 44920 5.6 U OYE3 SGDID:S000006092, Chr XVI from 227370-226168, reverse complement, Verified ORF, "Widely conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with slight differences in ligand binding and catalytic properties; may be involved in sterol metabolism" 02142006-Holinger-03_itms_6.05132.05132.2 2.0587 0.1134 92.5 1049.5402 1050.1998 5596.0 113 4.214 62.5 1 V.ADVVELTFR.A Reverse_YMR094W 1 1 2.1% 478 56336 9.1 U CTF13 SGDID:S000004700, Chr XIII from 455824-457260, Verified ORF, "Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis" * 02142006-Holinger-04_itms_6.05217.05217.2 2.5875 0.2156 99.4 1205.6545 1206.4056 7404.7 439 5.373 55.6 1 G.TRSIQNMRSL.R Reverse_YLR337C 1 1 2.0% 817 82594 9.9 U VRP1 SGDID:S000004329, Chr XII from 805106-802653, reverse complement, Verified ORF, "Proline-rich, actin-associated protein involved in cytoskeletal organization and cytokinesis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)" * 02142006-Holinger-06_itms_19.12945.12945.2 1.9147 0.221 97.1 1561.8734 1563.8369 5033.6 33 4.526 40.0 1 V.NPLSPPPPATPPLPPA.H YHR015W 1 1 2.0% 659 75920 9.4 U MIP6 SGDID:S000001057, Chr VIII from 134547-136526, Verified ORF, "Putative RNA-binding protein, interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export" * 02142006-Holinger-03_itms_5.04364.04364.2 2.4046 0.1943 98.2 1471.7794 1474.6965 5456.1 1 4.641 54.2 1 L.DYFSTIGPIKSVF.I YML031W 1 1 2.0% 655 74134 8.3 U NDC1 SGDID:S000004493, Chr XIII from 214189-216156, Verified ORF, "Nuclear envelope protein with multiple putative transmembrane domains, required for nuclear pore complex assembly and spindle pole body duplication; required for meiosis II" * 02142006-Holinger-05_itms_8.06081.06081.2 4.0204 0.2853 100.0 1626.7941 1627.7966 5826.8 1 5.803 79.2 1 E.NFFQAPQDYVLER.V Reverse_YIR039C 1 1 2.0% 537 58214 4.3 U YPS6 SGDID:S000001478, Chr IX from 432107-430494, reverse complement, Verified ORF, "Putative GPI-anchored aspartic protease" * 02142006-Holinger-03_itms_12.08727.08727.2 1.7074 0.1203 97.5 1104.7222 1104.3502 3632.7 2 4.549 60.0 1 L.IAVSQATIIMG.P Reverse_YCR045C 1 1 2.0% 491 55068 5.4 U YCR045C SGDID:S000000641, Chr III from 209605-208130, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-05_itms_11.08424.08424.2 2.5033 0.1115 90.1 1275.6887 1276.4412 5622.5 60 4.114 66.7 1 V.KTCHEVIFEL.G YDL063C 1 1 1.9% 620 70164 5.2 U YDL063C SGDID:S000002221, Chr IV from 340134-338272, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-05_itms_12.08511.08511.2 2.1445 0.2279 97.3 1297.6708 1298.6061 4958.2 417 4.526 50.0 1 E.IAIDLITAIIEI.V YLL024C 2 2 1.9% 639 69470 5.1 U SSA2 SGDID:S000003947, Chr XII from 97484-95565, reverse complement, Verified ORF, "ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shock protein 70 (HSP70) family; associated with the chaperonin-containing T-complex; present in the cytoplasm, vacuolar membrane and cell wall" * 02142006-Holinger-06_itms_10.07282.07282.2 3.7725 0.3305 100.0 1362.7573 1363.5939 6296.7 1 6.605 81.8 1 S.LLSIEDGIFEVK.A * 02142006-Holinger-03_itms_6.04862.04862.2 1.9627 0.0687 98.9 1136.5867 1137.275 4721.8 97 4.808 61.1 1 L.SIEDGIFEVK.A Reverse_YDR458C 1 1 1.8% 663 76377 7.8 U YDR458C SGDID:S000002866, Chr IV from 1382036-1380045, reverse complement, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery" * 02142006-Holinger-06_itms_6.05194.05194.2 2.3587 0.1283 93.4 1357.6837 1358.7942 4904.5 49 4.255 59.1 1 Q.IKKILFLVGLTI.F Reverse_YIL155C 1 1 1.8% 649 72389 7.9 U GUT2 SGDID:S000001417, Chr IX from 53708-51759, reverse complement, Verified ORF, "Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner" * 02142006-Holinger-06_itms_16.11472.11472.2 1.6304 0.1661 95.8 1146.7457 1147.2737 4133.6 78 4.424 50.0 1 R.VVGQTASGKKGD.A YER020W 1 1 1.8% 449 50448 6.4 U GPA2 SGDID:S000000822, Chr V from 195167-196516, Verified ORF, "Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery" * 02142006-Holinger-02_itms_4.03490.03490.2 2.5641 0.0983 90.3 968.4945 969.03613 5171.3 7 4.122 78.6 1 S.LSEYDQTL.M Reverse_YNL298W 1 1 1.7% 842 93909 9.1 U CLA4 SGDID:S000005242, Chr XIV from 68915-71443, Verified ORF, "Cdc42p activated signal transducing kinase of the PAK (p21-activated kinase) family, involved in septin ring assembly and cytokinesis; directly phosphorylates septins Cdc3p and Cdc10p; other yeast PAK family members are Ste20p and Skm1p" * 02142006-Holinger-04_itms_7.06260.06260.2 2.8173 0.0062 91.0 1517.7671 1518.812 4143.8 2 4.155 57.7 1 Q.SPDANVTVKKLKSM.I YHL024W 1 1 1.7% 713 80109 5.8 U RIM4 SGDID:S000001016, Chr VIII from 56647-58788, Verified ORF, "Putative RNA-binding protein required for the expression of early and middle sporulation genes" * 02142006-Holinger-01_itms_16.10232.10232.2 1.708 0.1803 94.2 1167.7074 1168.3502 2229.1 179 4.306 45.5 1 P.PPSGLDGSMIPP.P YML020W 1 1 1.7% 664 76138 8.1 U YML020W SGDID:S000004482, Chr XIII from 231149-233143, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-04_itms_6.05242.05242.2 2.1799 0.2163 92.3 1189.5491 1192.4008 5684.7 1 4.208 70.0 1 I.ISNNVKITFVG.S YDR049W 1 1 1.7% 632 72733 8.9 U YDR049W SGDID:S000002456, Chr IV from 553251-555149, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-02_itms_17.10998.10998.2 2.3429 0.1506 90.7 1276.6919 1277.4601 3950.0 105 4.141 55.0 1 E.TLIEQAVNFLE.H YPR036W 1 1 1.7% 478 54416 6.4 U VMA13 SGDID:S000006240, Chr XVI from 643833-645269, Verified ORF, "Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase" * 02142006-Holinger-01_itms_26.15955.15955.2 1.5665 0.0264 91.6 887.7318 885.9927 2931.3 19 4.174 78.6 1 L.IHLLSTSD.N Reverse_YPL161C 1 2 1.6% 633 70993 5.0 U BEM4 SGDID:S000006082, Chr XVI from 246219-244318, reverse complement, Verified ORF, "Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p" * 02142006-Holinger-04_itms_8.06563.06563.2 2.8893 0.1066 92.3 1088.6064 1090.3325 4989.9 8 4.209 77.8 2 S.NCLIMLAAAL.A Reverse_YDL003W 1 1 1.6% 566 63290 4.6 U MCD1 SGDID:S000002161, Chr IV from 444680-446380, Verified ORF, "Essential protein required for sister chromatid cohesion in mitosis and meiosis; subunit of the cohesin complex; expression is cell cycle regulated and peaks in S phase " * 02142006-Holinger-04_itms_14.10399.10399.2 1.7376 0.2033 91.8 926.68427 926.0605 2354.2 267 4.187 62.5 1 S.AVEGPTPRV.K YBL105C 1 1 1.5% 1151 131519 7.1 U PKC1 SGDID:S000000201, Chr II from 17696-14241, reverse complement, Verified ORF, "Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)" * 02142006-Holinger-05_itms_7.05963.05963.2 3.2384 0.069 92.9 2097.0261 2099.3916 6754.7 1 4.231 53.1 1 V.EKQYQEANTKLTKLYQI.D YER125W 1 1 1.5% 809 91816 6.7 U RSP5 SGDID:S000000927, Chr V from 410185-412614, Verified ORF, "Ubiquitin-protein ligase involved in ubiquitin-mediated protein degradation; plays a role in heat shock element (HSE)-mediated gene expression and multivesicular body sorting; contains a hect (homologous to E6-AP carboxyl terminus) domain" * 02142006-Holinger-06_itms_10.07835.07835.2 2.2165 0.194 99.1 1362.7133 1363.5559 4049.4 4 4.826 68.2 1 R.DVFRSPDPFAVL.T YPR175W 1 1 1.5% 689 78340 6.2 U DPB2 SGDID:S000006379, Chr XVI from 888970-891039, Verified ORF, "Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for normal yeast chromosomal replication; expression peaks at the G1/S phase boundary; potential Cdc28p substrate" * 02142006-Holinger-04_itms_12.09001.09001.2 1.7409 0.0338 90.6 1146.6442 1147.3195 3388.5 312 4.131 61.1 1 N.LLGRDAQNFL.L Reverse_YBR299W 1 1 1.5% 584 68142 5.7 U MAL32 SGDID:S000000503, Chr II from 805345-807099, Verified ORF, "Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C" Reverse_YGR292W 1 1 1.5% 584 68094 5.7 U MAL12 SGDID:S000003524, Chr VII from 1076605-1078359, Verified ORF, "Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus" 02142006-Holinger-01_itms_14.08778.08778.2 1.7035 0.1831 90.2 901.45557 901.0252 2958.5 35 4.115 62.5 1 S.GHAVEGVTM.I Reverse_YKL173W 1 1 1.4% 1008 114041 5.9 U SNU114 SGDID:S000001656, Chr XI from 122522-125548, Verified ORF, "GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2" * 02142006-Holinger-02_itms_6.05184.05184.2 3.0297 0.1617 92.6 1504.7482 1505.6648 7900.4 94 4.222 53.8 1 R.LDTEFGASEIVPVQ.G YIL108W 1 10 1.4% 696 77414 7.5 U YIL108W SGDID:S000001370, Chr IX from 160884-162974, Uncharacterized ORF, "Putative metalloprotease" * 02142006-Holinger-05_itms_7.05554.05554.2 3.9276 0.2657 99.3 1219.6621 1221.3989 5410.8 1 4.917 88.9 10 G.SKDIDLFNLR.E Reverse_YNR008W 1 1 1.4% 661 75393 6.7 U LRO1 SGDID:S000005291, Chr XIV from 640398-642383, Verified ORF, "Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis; putative homolog of human lecithin cholesterol acyltransferase" * 02142006-Holinger-05_itms_17.11437.11437.2 1.7002 0.2229 96.1 842.51263 843.01105 2224.7 310 4.472 56.2 1 E.IGTSIVGPV.M YEL036C 1 1 1.4% 500 58182 5.3 U ANP1 SGDID:S000000762, Chr V from 84552-83050, reverse complement, Verified ORF, "Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol" * 02142006-Holinger-03_itms_19.12170.12170.2 1.2386 0.0923 91.1 810.56726 812.76495 1917.4 7 4.155 66.7 1 I.YEPSSDD.L YMR280C 1 2 1.3% 1433 160485 9.0 U CAT8 SGDID:S000004893, Chr XIII from 831328-827027, reverse complement, Verified ORF, "Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements" * 02142006-Holinger-06_itms_8.06386.06386.2 2.8266 0.1424 92.6 1762.7714 1763.9047 9298.7 63 4.22 38.2 2 A.SADPGTNKKAVTNAGANF.K YKL215C 1 1 1.3% 1286 140427 6.8 U YKL215C SGDID:S000001698, Chr XI from 30688-26828, reverse complement, Uncharacterized ORF, "Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm" * 02142006-Holinger-05_itms_11.08337.08337.2 2.2646 0.1743 96.1 1779.885 1778.842 3914.2 361 4.471 34.4 1 K.SSPNEQEGILEGNSGEM.V Reverse_YPL160W 1 1 1.3% 1090 124141 5.8 U CDC60 SGDID:S000006081, Chr XVI from 246989-250261, Verified ORF, "Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA" * 02142006-Holinger-05_itms_13.09355.09355.2 2.0257 0.0879 96.6 1501.7227 1502.7942 3606.3 232 4.512 46.2 1 R.LYDLAALVGKDVPK.S Reverse_YNL267W 1 1 1.3% 1066 119923 6.5 U PIK1 SGDID:S000005211, Chr XIV from 140879-144079, Verified ORF, "Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokineses through the actin cytoskeleton" * 02142006-Holinger-05_itms_8.06314.06314.2 2.4556 0.1987 93.2 1485.0029 1484.5626 5417.0 21 4.252 50.0 1 F.HDNLSAIGGKDDLE.A YLR249W 1 1 1.3% 1044 115945 6.0 U YEF3 SGDID:S000004239, Chr XII from 636782-639916, Verified ORF, "Translational elongation factor, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing EF-1 alpha from the ribosomal complex; contains two ABC cassettes; binds and hydrolyses ATP" * 02142006-Holinger-06_itms_9.06834.06834.2 2.6395 0.161 98.9 1525.8683 1526.8162 5196.5 1 4.779 69.2 1 K.LVEDPQVIAPFLGK.L YDR475C 1 1 1.3% 876 98691 9.6 U YDR475C SGDID:S000002883, Chr IV from 1410084-1407454, reverse complement, Uncharacterized ORF, "Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence" * 02142006-Holinger-03_itms_7.05527.05527.2 3.3706 0.1515 91.8 1207.737 1206.3011 4675.1 8 4.177 75.0 1 V.KSNDSNSGKRI.K Reverse_YLL054C 1 1 1.3% 769 89163 7.8 U YLL054C SGDID:S000003977, Chr XII from 35203-32894, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-06_itms_6.05008.05008.2 1.573 0.1048 91.5 1095.543 1095.1974 5455.1 6 4.172 50.0 1 T.LNNSKVGFSE.K Reverse_YJL103C 1 1 1.3% 618 70382 7.9 U YJL103C SGDID:S000003639, Chr X from 230798-228942, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-01_itms_27.16482.16482.2 1.7509 0.1789 91.3 867.57007 869.9134 5435.2 33 4.165 71.4 1 T.IKSSTSSC.A Reverse_YKL204W 1 1 1.3% 632 69762 9.5 U EAP1 SGDID:S000001687, Chr XI from 53705-55603, Verified ORF, "eIF4E-associated protein, binds eIF4E and inhibits cap-dependent translation, also functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade" * 02142006-Holinger-01_itms_7.04895.04895.1 1.0454 0.2275 97.0 815.21497 814.87354 1969.8 1 4.972 50.0 1 S.KHTAAATD.S YKR095W 1 1 1.2% 1875 218454 5.2 U MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs" * 02142006-Holinger-06_itms_10.07492.07492.2 3.4127 0.3172 99.7 2369.2441 2370.6218 7678.4 1 5.377 40.9 1 Y.TAADKEVNNSTNGPGLNNILITL.R Reverse_YDR159W 1 1 1.2% 1301 149568 8.9 U SAC3 SGDID:S000002566, Chr IV from 771872-775777, Verified ORF, "Nuclear pore-associated protein, forms a complex with Thp1p that is involved in transcription and in mRNA export from the nucleus" * 02142006-Holinger-04_itms_10.07794.07794.2 2.6798 0.2464 97.9 1689.8328 1690.9086 5834.4 16 4.578 42.9 1 S.FILNKDSSSLMFSSN.V YDR017C 1 1 1.2% 1050 119550 6.8 U KCS1 SGDID:S000002424, Chr IV from 482263-479111, reverse complement, Verified ORF, "Inositol hexaphosphate kinase, phosphorylates inositol hexakisphosphate (InsP6) to diphosphoinositol polyphosphates, required for proper vacuole morphology and involved in salt stress response, contains two leucine heptad repeats" * 02142006-Holinger-05_itms_10.07737.07737.2 3.4237 0.1042 90.4 1563.8369 1565.776 7608.5 6 4.117 62.5 1 K.VNLIDFARCVTKE.D YNL278W 1 1 1.2% 1060 118280 8.9 U CAF120 SGDID:S000005222, Chr XIV from 113271-116453, Verified ORF, "Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation" * 02142006-Holinger-06_itms_10.07358.07358.2 2.4913 0.1755 91.1 1348.7443 1350.4711 7162.1 27 4.155 54.2 1 L.GSADLFERADGVL.S YFR029W 1 1 1.2% 678 76286 7.5 U PTR3 SGDID:S000001925, Chr VI from 210925-212961, Verified ORF, "Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes" * 02142006-Holinger-06_itms_7.05720.05720.2 1.4515 0.0999 98.2 955.5288 953.0422 2696.2 387 4.67 57.1 1 E.YGPNFNQL.T YNL299W 1 1 1.2% 642 74179 7.0 U TRF5 SGDID:S000005243, Chr XIV from 66517-68445, Verified ORF, "Poly (A) polymerase involved in nuclear RNA quality control based on: homology with Trf4p, genetic interactions with TRF4 mutants, physical interaction with Mtr4p (TRAMP subunit), and by direct assay; disputed role as a sigma DNA polymerase" * 02142006-Holinger-04_itms_7.06261.06261.2 2.2261 0.1298 92.0 926.54425 926.19055 4414.7 270 4.188 78.6 1 V.IVKTRVPI.I YPL242C 1 1 1.1% 1495 172830 8.9 U IQG1 SGDID:S000006163, Chr XVI from 95109-90622, reverse complement, Verified ORF, "Essential protein required for determination of budding pattern, promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family" * 02142006-Holinger-03_itms_5.04381.04381.2 2.7795 0.1226 96.1 1822.787 1824.0488 7454.8 46 4.467 43.3 1 K.DFSPVHKSKFGNFKNA.V Reverse_YMR129W 1 1 1.1% 1337 151652 6.7 U POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore complexes during S-phase;" * 02142006-Holinger-04_itms_7.06185.06185.2 3.1559 0.1072 90.5 1781.8633 1783.9817 4403.3 435 4.14 46.4 1 E.KSFLNETVKTIHHND.Q YKL197C 1 1 1.1% 1043 117276 7.0 U PEX1 SGDID:S000001680, Chr XI from 73870-70739, reverse complement, Verified ORF, "AAA-family ATPase peroxin required for peroxisome biogenesis, contains two 230 amino acid ATP-binding AAA cassettes, upregulated in anaerobiosis; Pex1p and Pex6p interact via their N-terminal AAA-cassettes" * 02142006-Holinger-05_itms_5.04634.04634.2 2.6744 0.2349 99.2 1195.5776 1198.2804 4208.9 1 5.064 70.0 1 Q.EFGIAVHSHNS.D Reverse_YLR014C 1 1 1.1% 904 102724 7.4 U PPR1 SGDID:S000004004, Chr XII from 174981-172267, reverse complement, Verified ORF, "Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of genes involved in uracil biosynthesis; activity may be modulated by interaction with Tup1p" * 02142006-Holinger-05_itms_6.05313.05313.2 1.8894 0.1379 92.8 1196.6329 1197.463 4093.9 9 4.229 66.7 1 P.IHFKTPLEVI.P YLR219W 1 1 1.1% 728 80530 9.1 U MSC3 SGDID:S000004209, Chr XII from 574153-576339, Verified ORF, "Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate" * 02142006-Holinger-05_itms_12.08812.08812.2 1.4258 0.2636 93.9 828.51276 830.7862 2607.6 75 4.278 57.1 1 Y.HTDAASDN.A Reverse_YKL213C 1 1 1.1% 715 79506 5.0 U DOA1 SGDID:S000001696, Chr XI from 34108-31961, reverse complement, Verified ORF, "WD repeat protein required for ubiquitin-mediated protein degradation, forms complex with Cdc48p, plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase" * 02142006-Holinger-01_itms_28.17167.17167.1 1.4359 0.2598 91.2 733.35406 733.7563 2939.2 8 4.925 57.1 1 K.KDNGTAGA.G Reverse_YMR280C 1 1 1.0% 1433 160485 9.0 U CAT8 SGDID:S000004893, Chr XIII from 831328-827027, reverse complement, Verified ORF, "Zinc cluster transcriptional activator necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements" * 02142006-Holinger-05_itms_7.05632.05632.2 3.336 0.2479 96.9 1679.792 1680.8855 5264.5 12 4.522 50.0 1 K.SDMNDKMLDLNSPLS.F YPL217C 1 1 1.0% 1183 135571 6.8 U BMS1 SGDID:S000006138, Chr XVI from 143170-139619, reverse complement, Verified ORF, "Essential conserved nucleolar GTP-binding protein required for synthesis of 40S ribosomal subunits and for processing of the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, has similarity to Tsr1p" * 02142006-Holinger-06_itms_11.08077.08077.2 1.9043 0.0949 93.4 1303.5824 1305.2108 6041.9 395 4.255 50.0 1 L.EDGNPSEQAEDN.S Reverse_YGL049C 1 1 1.0% 914 103899 7.6 U TIF4632 SGDID:S000003017, Chr VII from 409607-406863, reverse complement, Verified ORF, "Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); homologous to Tif4631p" * 02142006-Holinger-03_itms_5.04620.04620.2 1.7985 0.0601 96.4 1028.5641 1029.011 4427.7 250 4.493 68.8 1 R.NSSYRNSNS.Q Reverse_YIL169C 1 1 1.0% 995 99736 4.6 U YIL169C SGDID:S000001431, Chr IX from 26106-23119, reverse complement, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-02_itms_17.11201.11201.2 1.476 0.111 97.3 1077.7306 1075.1174 3623.5 393 4.537 55.6 1 T.YSQSSVTTTT.T YKL073W 1 1 1.0% 881 99572 5.3 U LHS1 SGDID:S000001556, Chr XI from 296074-298719, Verified ORF, "Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; member of the Hsp70 family; localizes to the lumen of the ER; regulated by the unfolded protein response pathway" * 02142006-Holinger-05_itms_6.05041.05041.2 2.4506 0.1662 95.8 955.5271 957.0306 3556.5 39 4.417 75.0 1 K.QFGGYGTNL.P YBR098W 1 2 1.0% 691 78764 7.5 U MMS4 SGDID:S000000302, Chr II from 441509-443584, Verified ORF, "Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination and DNA repair" * 02142006-Holinger-04_itms_6.05316.05316.2 2.4481 0.0784 90.6 807.4647 807.9219 3106.8 38 4.134 83.3 2 M.SQIVDFV.E YHL035C 1 1 0.9% 1592 180925 8.5 U YHL035C SGDID:S000001027, Chr VIII from 32754-27976, reverse complement, Uncharacterized ORF, "Protein of unknown function, member of the ATP-binding cassette (ABC) family, potential Cdc28p substrate" YLL048C 1 1 0.8% 1661 189161 8.1 U YBT1 SGDID:S000003971, Chr XII from 46264-41279, reverse complement, Verified ORF, "Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mammalian bile transporters" 02142006-Holinger-01_itms_17.10894.10894.2 1.8439 0.2374 93.6 1387.8408 1390.5333 3230.1 15 4.272 42.3 1 L.TEIGEKGITLSGGQ.K Reverse_YDR301W 1 1 0.9% 1357 153404 5.9 U CFT1 SGDID:S000002709, Chr IV from 1063346-1067419, Verified ORF, "RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF" * 02142006-Holinger-05_itms_17.11736.11736.2 2.1575 0.2341 98.7 1300.1857 1297.4045 3526.2 86 4.761 50.0 1 V.TSVTGSVEEQFI.E YPR049C 1 1 0.9% 1178 135025 5.7 U ATG11 SGDID:S000006253, Chr XVI from 664670-661134, reverse complement, Verified ORF, "Peripheral membrane protein required for delivery of aminopeptidase I (Lap4p) to the vacuole in the cytoplasm-to-vacuole targeting pathway; also required for peroxisomal degradation (pexophagy)" * 02142006-Holinger-03_itms_6.05150.05150.2 2.3151 0.1476 90.5 1256.595 1257.4473 5970.1 2 4.139 65.0 1 Q.MLDAHDNSLIK.S Reverse_YMR287C 1 1 0.9% 969 110822 9.0 U MSU1 SGDID:S000004900, Chr XIII from 845344-842435, reverse complement, Verified ORF, "RNase, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs" * 02142006-Holinger-04_itms_18.12199.12199.2 1.3195 0.0711 95.4 978.9877 980.10876 2462.3 30 4.359 56.2 1 K.LTGRAFSLD.P Reverse_YPR164W 1 1 0.8% 1407 161237 5.9 U MMS1 SGDID:S000006368, Chr XVI from 870699-874922, Verified ORF, "Protein likely involved in protection against replication-dependent DNA damage; mutants are sensitive to methyl methanesulfonate (MMS); implicated in regulation of Ty1 transposition" * 02142006-Holinger-04_itms_8.06319.06319.2 2.0793 0.1255 92.0 1207.6156 1206.2144 4432.3 121 4.194 50.0 1 V.QSNRTDNINSG.T Reverse_YDR310C 1 7 0.8% 1062 118201 6.2 U SUM1 SGDID:S000002718, Chr IV from 1084310-1081122, reverse complement, Verified ORF, "Transcriptional repressor required for repression of middle sporulation-specific genes during mitosis; regulated by the pachytene checkpoint; a dominant mutation acts as a suppressor of silencing defects of SIR2 mutations" * 02142006-Holinger-05_itms_15.10721.10721.2 1.9432 0.3392 98.2 960.5913 961.1241 4313.8 130 4.673 50.0 7 I.SASRPKPSM.S YKL210W 1 1 0.8% 1024 114266 5.1 U UBA1 SGDID:S000001693, Chr XI from 39164-42238, Verified ORF, "Ubiquitin activating enzyme, involved in ubiquitin-mediated protein degradation and essential for viability" * 02142006-Holinger-02_itms_7.05688.05688.1 2.1114 0.2225 100.0 850.4812 850.90356 2276.3 16 5.005 64.3 1 H.GLEDGNFV.R YCR057C 1 1 0.8% 923 103983 5.1 U PWP2 SGDID:S000000653, Chr III from 223223-220452, reverse complement, Verified ORF, "Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis" * 02142006-Holinger-04_itms_14.10105.10105.1 1.7572 0.123 91.4 766.5347 767.85956 2212.6 250 4.916 50.0 1 E.WLAFGSS.K Reverse_YBL088C 1 1 0.7% 2787 321567 6.9 U TEL1 SGDID:S000000184, Chr II from 59379-51016, reverse complement, Verified ORF, "Protein kinase, primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; homolog of human ataxia telangiectasia (ATM) gene" * 02142006-Holinger-06_itms_10.07350.07350.2 3.4213 0.1771 94.9 2150.0361 2151.5571 6836.0 2 4.336 41.7 1 I.ALQVYNALQIISGLRLYKS.C YGR109W-B 1 1 0.7% 1547 178419 8.8 U YGR109W-B SGDID:S000007347, Chr VII from 707614-708463,708465-712258, transposable_element_gene, "TyB Gag-Pol protein; proteolytically processed to make the Gag, RT, PR, and IN proteins that are required for retrotransposition" YIL082W-A 1 1 0.7% 1498 172454 9.2 U YIL082W-A SGDID:S000003537, Chr IX from 205632-206482,206484-210129, transposable_element_gene, "Hypothetical protein" 02142006-Holinger-04_itms_8.06662.06662.2 2.2967 0.1761 91.2 1243.6528 1244.4302 4276.2 103 4.16 65.0 1 A.QFPIPEEASIL.E YIL159W 1 2 0.7% 1375 156851 7.9 U BNR1 SGDID:S000001421, Chr IX from 41825-45952, Verified ORF, "Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNI1" 02142006-Holinger-05_itms_15.10440.10440.2 1.2866 0.0081 91.2 1004.6176 1006.2328 1897.9 36 4.157 50.0 2 P.PPPPPPPPLP.Q Reverse_YPR026W 1 1 0.7% 1211 136920 5.4 U ATH1 SGDID:S000006230, Chr XVI from 615376-619011, Verified ORF, "Acid trehalase required for utilization of extracellular trehalose" * 02142006-Holinger-03_itms_19.12394.12394.2 1.387 0.1706 93.9 966.5175 966.9395 3743.4 2 4.287 62.5 1 L.YSQSSSGHN.L YNL163C 1 1 0.7% 1110 124464 5.1 U RIA1 SGDID:S000005107, Chr XIV from 330075-326743, reverse complement, Verified ORF, "Cytoplasmic GTPase involved in biogenesis of the 60S ribosome; has similarity to translation elongation factor 2 (Eft1p and Eft2p)" * 02142006-Holinger-05_itms_18.12310.12310.2 1.9446 0.1407 93.6 876.5226 877.0311 4523.4 41 4.272 71.4 1 I.KNGFQLAV.S YJL005W 1 2 0.6% 2026 227832 7.3 U CYR1 SGDID:S000003542, Chr X from 425072-431152, Verified ORF, "Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; involved in cell cycle control and glucose and nitrogen repression of sporulation" * 02142006-Holinger-06_itms_11.07941.07941.2 3.18 0.1179 92.9 1303.5834 1304.4827 4927.3 2 4.242 63.6 2 N.ELESLPAGFVEL.K YML103C 1 1 0.6% 1655 188576 5.6 U NUP188 SGDID:S000004571, Chr XIII from 67549-62582, reverse complement, Verified ORF, "Subunit of the nuclear pore complex (NPC), involved in the structural organization of the complex and of the nuclear envelope, also involved in nuclear envelope permeability, interacts with Pom152p and Nic96p" * 02142006-Holinger-05_itms_5.04802.04802.2 2.3279 0.104 92.1 1057.5614 1059.2914 5216.5 227 4.197 61.1 1 I.TTILILGLNT.S YDR150W 1 1 0.4% 2748 313032 5.4 U NUM1 SGDID:S000002557, Chr IV from 755623-763869, Verified ORF, "Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex" * 02142006-Holinger-01_itms_26.15846.15846.2 1.5533 0.0379 92.2 999.64825 1001.1021 3681.9 286 4.203 61.1 1 A.SFMSRAGSAS.R YPR117W 1 1 0.4% 2489 285902 8.3 U YPR117W SGDID:S000006321, Chr XVI from 760023-767492, Uncharacterized ORF, "Hypothetical protein" * 02142006-Holinger-04_itms_18.12127.12127.2 1.2833 0.2596 95.8 969.69073 970.0239 2652.0 209 4.406 43.8 1 L.SFGSSLDEK.V Proteins Peptide IDs Spectra Unfiltered 10154 34401 51016 Filtered 167 351 562 Forward matches 118 302 501 Decoy matches 49 49 61 Forward FP rate 41.53 16.23 12.18 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0