DTASelect v2.0.21 /data/1/catclw/Projects/Jamie/December/TiO2_step345/splitted /garibaldi/people-b/applications/yates/dbase/SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta SEQUEST 3.0 in SQT format. --trypstat -e con --fp 0.1 -p 1 true Use criteria 0.0 Minimum peptide confidence 0.1 Peptide false positive rate 0.0 Minimum protein confidence 1.0 Protein false positive rate 1 Minimum charge state 16 Maximum charge state 0.0 Minimum ion proportion 1000 Maximum Sp rank -1.0 Minimum Sp score Include Modified peptide inclusion Any Tryptic status requirement false Multiple, ambiguous IDs allowed Ignore Peptide validation handling XCorr Purge duplicate peptides by protein false Include only loci with unique peptide true Remove subset proteins Ignore Locus validation handling con Exclude protein names matching 0 Minimum modified peptides per locus 1000 Minimum redundancy for low coverage loci 1 Minimum peptides per locus Locus Sequence Count Spectrum Count Sequence Coverage Length MolWt pI Validation Status Descriptive Name Unique FileName XCorr DeltCN Conf% M+H+ CalcM+H+ TotalIntensity SpR ZScore IonProportion Redundancy Sequence YOR257W 3 8 40.4% 161 18751 4.6 U CDC31 SGDID:S000005783, Chr XV from 811006-811491, Verified ORF, "Component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; interacts with Kar1p" * Jamie_03_itms_8.16444.16444.3 3.2336 0.1787 100.0 2940.0244 2940.21 8850.9 356 4.999 20.7 1 K.QEIYEAFSLFDMNNDGFLDYHELK.V * Jamie_04_itms_3.22063.22063.3 3.9788 0.3493 100.0 4170.054 4170.5425 7509.0 1 5.517 20.1 1 R.VAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAI.C * Jamie_05_itms_4.20943.20943.3 3.7047 0.3179 100.0 4633.014 4633.953 4624.2 1 6.759 24.4 6 R.VAKELGETLTDEELRAMIEEFDLDGDGEINENEFIAICTDS.- YBR109C 2 6 25.2% 147 16135 4.3 U CMD1 SGDID:S000000313, Chr II from 458356-457913, reverse complement, Verified ORF, "Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin" * Jamie_04_itms_4.23386.23386.4 3.2677 0.2079 100.0 4107.9766 4109.526 7621.3 37 4.158 18.1 1 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q * Jamie_03_itms_13.20957.20957.3 5.3755 0.5193 100.0 4108.494 4109.526 6128.2 1 8.643 27.8 5 R.SLGLSPSEAEVNDLMNEIDVDGNHQIEFSEFLALMSR.Q YOR286W 1 1 18.8% 149 16697 9.6 U YOR286W SGDID:S000005812, Chr XV from 850277-850726, Uncharacterized ORF, "The authentic, non-tagged protein was localized to the mitochondria" * Jamie_03_itms_8.16226.16226.3 2.8655 0.2015 100.0 3026.9043 3027.5405 10416.3 334 3.914 21.3 1 K.HSTGILSRTVSARSPTLVLRTFTTKAPK.I YPL124W 4 5 15.0% 253 29280 9.5 U SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication" * Jamie_03_itms_2.10677.10677.4 4.5493 0.2207 100.0 4382.0566 4382.572 5579.6 48 4.227 18.0 1 R.SSDNINKEGAREDRSSQIHIENEST*EDILKILSSSFHN.- * Jamie_03_itms_2.05408.05408.2 3.6077 0.3247 100.0 1923.0521 1923.9855 5081.8 1 5.518 63.3 2 R.SSQIHIENEST*EDILK.I * Jamie_03_itms_4.13572.13572.3 3.1523 0.1963 100.0 2809.5544 2809.9604 7181.4 64 4.809 26.1 1 R.SSQIHIENES*TEDILKILSSSFHN.- * Jamie_03_itms_4.13151.13151.3 3.2397 0.2378 100.0 2889.2344 2889.9604 6503.2 180 4.376 25.0 1 R.SSQIHIENEST*EDILKILSS*SFHN.- YIL061C 1 1 13.3% 300 34447 10.0 U SNP1 SGDID:S000001323, Chr IX from 245556-244654, reverse complement, Verified ORF, "Component of U1 snRNP required for mRNA splicing via spliceosome; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein" * Jamie_03_itms_25.28871.28871.4 1.7887 0.5369 100.0 4371.3765 4373.6104 6095.7 81 2.908 10.7 1 S.SS*AYSADRYGSSTLDARYRGNRPLLSAATPTAAVTSVY*KS.R YFR028C 2 2 12.7% 551 61907 8.0 U CDC14 SGDID:S000001924, Chr VI from 210056-208401, reverse complement, Verified ORF, "Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit" * Jamie_03_itms_17.24023.24023.4 4.4694 0.4213 100.0 5196.5366 5197.7397 6087.4 1 7.545 18.2 1 R.VYLGAYDYTPEDTDELVFFTVEDAIFYNSFHLDFGPMNIGHLYR.F * Jamie_03_itms_5.14398.14398.3 3.8496 0.3398 100.0 3012.2644 3013.3398 8389.9 20 5.711 26.0 1 K.HFEDIGIQHLDLIFEDGTCPDLSIVK.N YKL060C 1 1 12.3% 359 39621 5.8 U FBA1 SGDID:S000001543, Chr XI from 327131-326052, reverse complement, Verified ORF, "Fructose 1,6-bisphosphate aldolase, a cytosolic enzyme required for glycolysis and gluconeogenesis; catalyzes the conversion of fructose 1,6 bisphosphate into two 3-carbon products: glyceraldehyde-3-phosphate and dihydroxyacetone phosphate" * Jamie_03_itms_6.14632.14632.4 4.0067 0.3206 100.0 5038.9365 5040.5103 5972.9 1 5.629 18.2 1 R.MAAMDQWLEMEIGITGGEEDGVNNENADKEDLYTKPEQVYNVYK.A YPL131W 1 3 10.4% 297 33715 6.8 U RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 ribosomal proteins; binds 5S rRNA and is required for 60S subunit assembly" * Jamie_05_itms_3.14011.14011.3 6.3056 0.5109 100.0 3500.6943 3500.7944 6387.6 1 8.748 30.8 3 K.LGLDETYKGVEEVEGEYELTEAVEDGPRPFK.V YNR040W 1 1 9.4% 256 28636 10.1 U YNR040W SGDID:S000005323, Chr XIV from 699692-700462, Uncharacterized ORF, "Hypothetical protein" * Jamie_04_itms_5.26973.26973.3 2.0181 0.2119 100.0 2584.1343 2579.9163 8487.1 53 4.393 19.6 1 K.TFGPVALGVLPVILAAATKHVT*S*R.L Reverse_YBR192W 1 1 9.3% 377 42102 9.6 U RIM2 SGDID:S000000396, Chr II from 607647-608780, Verified ORF, "Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family" * Jamie_03_itms_5.14433.14433.4 3.2225 0.1449 93.9 4097.7363 4093.6348 11114.0 15 3.51 19.6 1 K.FLSRFGEQKYVNGIIGLTEKFHTGAQIVY*NISKPR.T YLR332W 1 1 8.2% 376 39146 5.7 U MID2 SGDID:S000004324, Chr XII from 790676-791806, Verified ORF, "O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p" * Jamie_03_itms_4.13120.13120.3 2.1924 0.4249 100.0 3074.2444 3076.801 8611.9 3 2.605 21.7 1 F.SFSSDSSTSSSSSASS*DSSSSSSFSISSTS*A.T YNL064C 1 1 8.1% 409 44671 6.3 U YDJ1 SGDID:S000005008, Chr XIV from 507098-505869, reverse complement, Verified ORF, "Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein translocation across membranes; member of the DnaJ family" * Jamie_04_itms_12.32153.32153.3 2.9878 0.2078 100.0 3564.4143 3563.8965 3848.9 10 5.104 18.0 1 R.DGDDLVYEAEIDLLTAIAGGEFALEHVSGDWLK.V Reverse_YKL141W 1 1 8.1% 198 22068 10.2 U SDH3 SGDID:S000001624, Chr XI from 179672-180268, Verified ORF, "Cytochrome b subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone" * Jamie_03_itms_16.22864.22864.2 1.5115 0.1493 94.1 1796.9122 1795.9921 7491.4 257 3.785 36.7 1 R.IAGGYHIAFLYAFS*GK.I YGL075C 1 1 7.2% 387 44585 8.3 U MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required for insertion of the newly duplicated SPB into the nuclear envelope; potentially phosphorylated by Cdc28p" * Jamie_03_itms_13.20917.20917.3 3.0255 0.2455 100.0 3119.1843 3118.4395 8522.8 1 4.517 26.9 1 R.EILENAFIGVDSIPSDFIVSMNLNSPS*K.F YNL225C 1 2 5.9% 581 67400 6.0 U CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration" * Jamie_03_itms_2.09894.09894.4 5.9006 0.3744 100.0 4204.4966 4205.8145 7801.1 5 6.177 21.2 2 K.ILCDKFDKLTIDEKEILKGCNEEIKIKLERLNER.L YGR234W 1 1 5.3% 399 44646 6.3 U YHB1 SGDID:S000003466, Chr VII from 959908-961107, Verified ORF, "Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses" * Jamie_04_itms_9.29866.29866.2 1.2028 0.2466 100.0 2231.372 2228.381 4305.8 198 4.185 17.5 1 R.T*NQKVGAQPNALAT*TVLAAAK.N YJR053W 3 4 5.2% 574 66087 5.9 U BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis" * Jamie_03_itms_3.11003.11003.3 2.3741 0.2648 100.0 3531.9543 3535.8586 9673.0 49 4.906 19.0 1 K.KLASWFIPRDET*IISVDEETIMDESTVNSK.R * Jamie_03_itms_3.11003.11003.2 2.0532 0.26 100.0 2354.9722 2356.5146 9673.0 1 4.297 40.0 2 R.DETIISVDEETIMDESTVNSK.R * Jamie_03_itms_3.10922.10922.3 3.1081 0.2402 100.0 2356.0144 2356.5146 10056.1 35 4.742 30.0 1 R.DETIISVDEETIMDESTVNSK.R Reverse_YBL097W 1 1 3.7% 754 86230 4.7 U BRN1 SGDID:S000000193, Chr II from 40828-43092, Verified ORF, "Essential protein required for chromosome condensation, likely to function as an intrinsic component of the condensation machinery, may influence multiple aspects of chromosome transmission and dynamics" * Jamie_03_itms_5.14033.14033.4 2.8478 0.161 91.2 3528.2966 3524.9624 8068.2 9 4.179 22.8 1 K.FLPDIILEQDLEKMKIT*EFEVLT*TELVR.N YOL122C 1 1 3.3% 575 63264 6.6 U SMF1 SGDID:S000005482, Chr XV from 91418-89691, reverse complement, Verified ORF, "Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins" * Jamie_05_itms_6.30571.30571.2 2.8077 0.2633 100.0 2143.872 2142.3523 9429.0 3 4.81 36.1 1 G.T*IFMLALLLSGQSAGVVCT*.M YGL006W 1 1 3.0% 1173 130861 7.4 U PMC1 SGDID:S000002974, Chr VII from 485925-489446, Verified ORF, "Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium" * Jamie_03_itms_23.27418.27418.4 3.3223 0.1672 100.0 4282.7363 4280.5713 8137.0 22 3.365 17.2 1 K.YLKTDKNAGIS*LPEISNYRKTNRYKNY*GDNSLPER.I YLR188W 1 1 3.0% 695 75950 9.8 U MDL1 SGDID:S000004178, Chr XII from 528302-530389, Verified ORF, "Half-type ATP-binding cassette (ABC) transporter of the inner mitochondrial membrane, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress" * Jamie_03_itms_12.20087.20087.3 2.7889 0.1717 100.0 2370.7444 2372.871 12016.7 12 4.126 30.0 1 K.LT*CVMMILAPPLGAMALIYGR.K Reverse_YOL087C 1 1 2.9% 1116 125382 6.7 U YOL087C SGDID:S000005447, Chr XV from 158636-155286, reverse complement, Uncharacterized ORF, "Hypothetical protein" * Jamie_03_itms_13.20778.20778.4 3.3418 0.1423 97.0 3877.6565 3876.2297 7358.7 322 4.558 18.8 1 K.DKNNKKS*ESNYGTDLTLSNT*LDKKKLSFIKKR.L YBR133C 1 1 2.7% 827 95153 6.4 U HSL7 SGDID:S000000337, Chr II from 504281-501798, reverse complement, Verified ORF, "Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization and periodic Hsl1p-dependent phosphorylation; required along with Hsl1p for bud neck recruitment, phosphorylation, and degradation of Swe1p" * Jamie_03_itms_4.13165.13165.2 2.9531 0.2656 100.0 2581.3523 2581.6624 8778.6 1 5.602 38.1 1 K.DTVQDEDDFTVEFSQSSLNEFK.I YAL047C 1 1 2.7% 622 72105 5.1 U SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization" * Jamie_03_itms_4.13773.13773.2 2.8545 0.2334 100.0 1998.4321 1999.1761 10051.3 1 6.311 53.1 1 R.ETLEETLELSSDYVLEK.M YOR048C 1 1 2.4% 1006 115934 6.8 U RAT1 SGDID:S000005574, Chr XV from 421650-418630, reverse complement, Verified ORF, "Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as mRNA transcription termination" * Jamie_03_itms_15.22212.22212.3 2.627 0.1708 94.1 2862.2944 2866.0522 7760.2 1 3.368 28.3 1 K.TY*MT*CDGVLNLPSVETLLQHLGSR.E YOR373W 2 8 2.2% 851 94104 7.0 U NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole body outer plaque, required for exit from mitosis" * Jamie_03_itms_2.07026.07026.2 3.8731 0.3226 100.0 2142.9521 2143.2659 7755.3 1 7.153 50.0 2 R.VEDITSISEVNTSFNETEK.Q * Jamie_03_itms_2.07278.07278.2 3.9202 0.3824 100.0 2222.6921 2223.2659 6630.0 1 6.488 58.3 6 R.VEDITSISEVNTS*FNETEK.Q YMR164C 1 1 2.2% 758 85050 7.8 U MSS11 SGDID:S000004774, Chr XIII from 589549-587273, reverse complement, Verified ORF, "Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of MUC1 and STA2 in response to nutritional signals" * Jamie_05_itms_9.34967.34967.2 1.197 0.3509 100.0 2020.9321 2023.8491 5775.4 311 5.022 18.8 1 N.TRNNTAEET*TPT*NDNNA.N YDR135C 1 1 2.0% 1515 171120 8.4 U YCF1 SGDID:S000002542, Chr IV from 727546-722999, reverse complement, Verified ORF, "Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin; similar to human cystic fibrosis protein CFTR" * Jamie_03_itms_10.18762.18762.4 3.7419 0.1428 100.0 3472.6165 3474.8064 9700.0 1 4.723 23.3 1 R.ESSIPVEGELEQLQKLNDLDFGNSDAISLRR.A YOR069W 1 1 1.9% 675 76484 5.2 U VPS5 SGDID:S000005595, Chr XV from 453769-455796, Verified ORF, "Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p" * Jamie_03_itms_16.23249.23249.2 1.6998 0.1407 100.0 1778.2922 1778.9193 6286.7 23 4.379 50.0 1 K.EFQT*LERRYNLTK.K YLR413W 1 1 1.8% 675 72698 5.1 U YLR413W SGDID:S000004405, Chr XII from 951152-953179, Uncharacterized ORF, "Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery" * Jamie_04_itms_18.38175.38175.1 1.2389 0.255 100.0 1329.0 1329.6294 1364.6 30 3.91 31.8 1 I.LLPICALLTLTT.F YMR092C 1 1 1.5% 615 67326 5.6 U AIP1 SGDID:S000004698, Chr XIII from 453478-451631, reverse complement, Verified ORF, "Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to restrict cofilin localization to cortical patches; contains WD repeats" * Jamie_04_itms_18.38208.38208.1 1.2228 0.2408 100.0 979.04 978.9023 3191.3 42 4.404 37.5 1 T.PSTLVS*S*GA.D YPL058C 1 1 1.1% 1511 171064 6.8 U PDR12 SGDID:S000005979, Chr XVI from 450374-445839, reverse complement, Verified ORF, "Plasma membrane weak-acid-inducible ATP-binding cassette (ABC) transporter, required for weak organic acid resistance, strongly induced by sorbate and benzoate, regulated by War1p, mutants exhibit sorbate hypersensitivity" * Jamie_04_itms_7.28590.28590.2 1.6759 0.3138 100.0 1909.8121 1911.9427 9865.5 21 5.692 37.5 1 M.DNNTGYLVNPTATENCQ.Y YDL112W 1 1 1.1% 1436 165047 6.1 U TRM3 SGDID:S000002270, Chr IV from 258915-263225, Verified ORF, "2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs" * Jamie_05_itms_5.27717.27717.2 1.2038 0.2356 100.0 2065.7322 2064.133 6481.0 233 3.797 23.3 1 K.LEEHAQT*IISLSY*SRR.S YMR176W 1 1 0.6% 1411 162700 6.8 U ECM5 SGDID:S000004788, Chr XIII from 611739-615974, Verified ORF, "Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibits cellular volume up to four times greater than wild-type, also large drooping buds with elongated necks" * Jamie_04_itms_19.38487.38487.1 1.1011 0.248 100.0 1004.89 1006.2328 3739.3 165 4.178 35.7 1 T.IPLKVSYW.T Proteins Peptide IDs Spectra Unfiltered 10593 54665 67160 Filtered 34 44 64 Forward matches 30 40 60 Decoy matches 4 4 4 Forward FP rate 13.33 10.0 6.67 Classification Nonredundant Proteins Redundant Proteins Unclassified 0 0