Abstract
Development of yeast meiotic chromosome cores into full-length synaptonemal complexes requires the MEK1 gene product, a meiosis-specific protein kinase homolog. The Mek1 protein associates with meiotic chromosomes and colocalizes with the Red1 protein, which is a component of meiotic chromosome cores. Mek1 and Red1 interact physically in meiotic cells, as demonstrated by coimmunoprecipitation and the two-hybrid protein system. Hop1, another protein associated with meiotic chromosome cores, also interacts with Mek1 but only in the presence of Red1. Red1 displays Mek1-dependent phosphorylation, both in vitro and in vivo, and Mek1 kinase activity is necessary for Mek1 function in vivo. Fluorescent in situ hybridization analysis indicates that Mek1-mediated phosphorylation of Red1 is required for meiotic sister-chromatid cohesion, raising the possibility that cohesion is regulated by protein phosphorylation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatids / genetics*
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Chromosomes / genetics*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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In Situ Hybridization, Fluorescence
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MAP Kinase Kinase 1
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Macromolecular Substances
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Meiosis / genetics*
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Mitogen-Activated Protein Kinase Kinases*
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Nuclear Proteins
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Phosphoproteins / genetics
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Phosphoproteins / metabolism
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Phosphorylation
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Protein Binding / genetics
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Protein Kinases / genetics
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Protein Kinases / metabolism
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Protein Serine-Threonine Kinases / genetics*
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Protein-Tyrosine Kinases / genetics*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Synaptonemal Complex / genetics*
Substances
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DNA-Binding Proteins
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Fungal Proteins
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HOP1 protein, S cerevisiae
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Macromolecular Substances
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Nuclear Proteins
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Phosphoproteins
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RED1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Zip1 protein, S cerevisiae
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Protein Kinases
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Protein-Tyrosine Kinases
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Protein Serine-Threonine Kinases
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MAP Kinase Kinase 1
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Mitogen-Activated Protein Kinase Kinases