Synaptonemal complex morphogenesis and sister-chromatid cohesion require Mek1-dependent phosphorylation of a meiotic chromosomal protein

Genes Dev. 1998 Nov 15;12(22):3551-63. doi: 10.1101/gad.12.22.3551.

Abstract

Development of yeast meiotic chromosome cores into full-length synaptonemal complexes requires the MEK1 gene product, a meiosis-specific protein kinase homolog. The Mek1 protein associates with meiotic chromosomes and colocalizes with the Red1 protein, which is a component of meiotic chromosome cores. Mek1 and Red1 interact physically in meiotic cells, as demonstrated by coimmunoprecipitation and the two-hybrid protein system. Hop1, another protein associated with meiotic chromosome cores, also interacts with Mek1 but only in the presence of Red1. Red1 displays Mek1-dependent phosphorylation, both in vitro and in vivo, and Mek1 kinase activity is necessary for Mek1 function in vivo. Fluorescent in situ hybridization analysis indicates that Mek1-mediated phosphorylation of Red1 is required for meiotic sister-chromatid cohesion, raising the possibility that cohesion is regulated by protein phosphorylation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatids / genetics*
  • Chromosomes / genetics*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • In Situ Hybridization, Fluorescence
  • MAP Kinase Kinase 1
  • Macromolecular Substances
  • Meiosis / genetics*
  • Mitogen-Activated Protein Kinase Kinases*
  • Nuclear Proteins
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Phosphorylation
  • Protein Binding / genetics
  • Protein Kinases / genetics
  • Protein Kinases / metabolism
  • Protein Serine-Threonine Kinases / genetics*
  • Protein-Tyrosine Kinases / genetics*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Synaptonemal Complex / genetics*

Substances

  • DNA-Binding Proteins
  • Fungal Proteins
  • HOP1 protein, S cerevisiae
  • Macromolecular Substances
  • Nuclear Proteins
  • Phosphoproteins
  • RED1 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Zip1 protein, S cerevisiae
  • Protein Kinases
  • Protein-Tyrosine Kinases
  • Protein Serine-Threonine Kinases
  • MAP Kinase Kinase 1
  • Mitogen-Activated Protein Kinase Kinases