Abstract
The PEL1/PGS1 gene of the yeast Saccharomyces cerevisiae is essential for the viability of rho-/rho degrees mutants and the normal cardiolipin content of cells. The PEL1-GFP fusion gene has been found to complement the pel1/pgs1 mutation and its fluorescent protein was localized to mitochondria similarly to the beta-galactosidase activity of a protein encoded by the PEL1-lacZ fusion gene. The expression of the PEL1-lacZ reporter gene was repressed in cells grown in the presence of inositol and choline, reduced in the ino2 and ino4 strains, but constitutive in the opi1 null-mutant strain. The results demonstrate that Pel1p, playing a vital role in cells impaired in the mitochondrial DNA, is localized in the mitochondria and expressed in response to inositol and choline.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Choline / pharmacology
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Endopeptidase K / metabolism
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Gene Expression Regulation, Fungal / drug effects*
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Genes, Reporter / genetics
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Genetic Complementation Test
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Green Fluorescent Proteins
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Immunohistochemistry
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Inositol / pharmacology
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Lac Operon / genetics
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Luminescent Proteins / genetics
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Mitochondria / enzymology*
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Mitochondria / genetics
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Mutagenesis / genetics
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Recombinant Fusion Proteins / genetics
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
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Transferases (Other Substituted Phosphate Groups) / genetics*
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Transformation, Genetic / genetics
Substances
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Luminescent Proteins
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Recombinant Fusion Proteins
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Green Fluorescent Proteins
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Inositol
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Transferases (Other Substituted Phosphate Groups)
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CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase
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Endopeptidase K
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Choline