Abstract
C. elegans cki-1 encodes a member of the CIP/KIP family of cyclin-dependent kinase inhibitors, and functions to link postembryonic developmental programs to cell cycle progression. The expression pattern of cki-1::GFP suggests that cki-1 is developmentally regulated in blast cells coincident with G1, and in differentiating cells. Ectopic expression of CKI-1 can prematurely arrest cells in G1, while reducing cki-1 activity by RNA-mediated interference (RNAi) causes extra larval cell divisions, suggesting a role for cki-1 in the developmental control of G1/S. cki-1 activity is required for the suspension of cell cycling that occurs in dauer larvae and starved L1 larvae in response to environmental signals. In vulva precursor cells (VPCs), a pathway of heterochronic genes acts via cki-1 to maintain VPCs in G1 during the L2 stage.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Caenorhabditis elegans / genetics
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Caenorhabditis elegans / growth & development*
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Caenorhabditis elegans Proteins*
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Cell Cycle Proteins / genetics*
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Cell Cycle Proteins / physiology
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Cell Differentiation
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Cell Division
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Cyclin-Dependent Kinase Inhibitor Proteins
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclin-Dependent Kinase Inhibitor p27
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Cyclin-Dependent Kinases / antagonists & inhibitors*
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Cyclins / genetics*
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Cyclins / physiology
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Enzyme Inhibitors*
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G1 Phase
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Gene Expression Regulation, Developmental*
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Genes, Helminth
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Genes, cdc
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Gonads / cytology
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Gonads / growth & development
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Green Fluorescent Proteins
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Luminescent Proteins / biosynthesis
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Luminescent Proteins / genetics
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Microtubule-Associated Proteins / genetics*
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Microtubule-Associated Proteins / physiology
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / metabolism
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S Phase
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Tumor Suppressor Proteins*
Substances
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Caenorhabditis elegans Proteins
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Cell Cycle Proteins
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Cyclin-Dependent Kinase Inhibitor Proteins
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins
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Enzyme Inhibitors
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Luminescent Proteins
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Microtubule-Associated Proteins
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Recombinant Fusion Proteins
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Tumor Suppressor Proteins
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cki-1 protein, C elegans
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Green Fluorescent Proteins
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Cyclin-Dependent Kinase Inhibitor p27
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Cyclin-Dependent Kinases