Abstract
The hop2 mutant of S. cerevisiae displays a novel phenotype: meiotic chromosomes form nearly wild-type amounts of synaptonemal complex, but most chromosomes are engaged in synapsis with nonhomologous partners. The meiosis-specific Hop2 protein localizes to chromosomes prior to and during synapsis and in the absence of the double-strand breaks that initiate recombination. hop2 strains sustain a wild-type level of meiotic double-strand breaks, but these breaks remain unrepaired. The hop2 mutant arrests at the pachytene stage of meiotic prophase with the RecA-like protein Dmc1 located at numerous sites along synapsed chromosomes. We propose that the Hop2 protein functions to prevent synapsis between nonhomologous chromosomes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Cycle Proteins*
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Chromosomes / genetics*
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Cloning, Molecular
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DNA Damage
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Fungal Proteins / chemistry*
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Fungal Proteins / genetics*
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Fungal Proteins / isolation & purification
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Fungal Proteins / physiology*
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Gene Conversion
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Gene Expression Regulation, Fungal
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Meiosis / genetics*
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Molecular Sequence Data
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Mutation
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Synaptonemal Complex / genetics
Substances
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Cell Cycle Proteins
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DMC1 protein, S cerevisiae
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DNA-Binding Proteins
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Fungal Proteins
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HOP2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins