Hexokinase PII has a double cytosolic-nuclear localisation in Saccharomyces cerevisiae

F Randez-Gil; P Herrero; P Sanz; J A Prieto; F Moreno

doi:10.1016/s0014-5793(98)00289-0

Hexokinase PII has a double cytosolic-nuclear localisation in Saccharomyces cerevisiae

FEBS Lett. 1998 Apr 3;425(3):475-8. doi: 10.1016/s0014-5793(98)00289-0.

Authors

F Randez-Gil¹, P Herrero, P Sanz, J A Prieto, F Moreno

Affiliation

¹ Departamento de Biotecnología de Alimentos, Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Burjassot Valencia, Spain.

PMID: 9563516
DOI: 10.1016/s0014-5793(98)00289-0

Abstract

We describe here that the HXK2 gene product, isoenzyme PII of hexokinase, is located in both the nucleus and the cytoplasm of Saccharomyces cerevisiae cells. This conclusion is supported by assays of hexokinase-specific activity in isolated nuclei from wild-type and hxk1lhxk2 double mutant strains, by immunoblot experiments using anti-Hxk2 antibodies and by observation of the fluorescence distribution of a Hxk2-GFP fusion protein in cells transformed with the HXK2::gfp gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Fractionation
Cell Nucleus / enzymology
Cytoplasm / enzymology
Enzyme Repression / physiology
Fluorescent Antibody Technique
Fungal Proteins / metabolism
Glucose / metabolism
Hexokinase / metabolism*
Isoenzymes / metabolism
Mutation / genetics
Phosphorylation
Protoplasts / enzymology
Recombinant Proteins / metabolism
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae / genetics

Substances

Fungal Proteins
Isoenzymes
Recombinant Proteins
Hexokinase
Glucose