Abstract
Four putative yeast transcription factors (Hal6-9p) have been identified which upon overexpression in multicopy plasmids increase sodium and lithium tolerance. This effect is mediated, at least in part, by increased expression of the Enalp Na+/Li+ extrusion pump. Hal6p and Hal7p are bZIP proteins and their gene disruptions affected neither salt tolerance nor ENA1 expression. Hal8p and Hal9p are putative zinc fingers and their gene disruptions decreased both salt tolerance and ENA1 expression. Therefore, Hal8p and Hal9p, but not Hal6p and Hal7p, qualify as transcriptional activators of ENA1 under physiological conditions. Hal8p seems to mediate the calcineurin-dependent part of ENA1 expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptation, Physiological
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Adenosine Triphosphatases / genetics
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Amino Acid Sequence
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Cation Transport Proteins*
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Cell Division
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Chromosome Mapping
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Culture Media
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal
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Leucine Zippers
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Lithium / pharmacology*
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Molecular Sequence Data
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Sodium / pharmacology*
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Sodium-Potassium-Exchanging ATPase
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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Cation Transport Proteins
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Culture Media
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DNA-Binding Proteins
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ENA1 protein, S cerevisiae
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Lithium
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Sodium
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Adenosine Triphosphatases
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Sodium-Potassium-Exchanging ATPase