Loss of the plasma membrane-bound protein Gas1p in Saccharomyces cerevisiae results in the release of beta1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity

J Bacteriol. 1998 Mar;180(6):1418-24. doi: 10.1128/JB.180.6.1418-1424.1998.

Abstract

Deletion of GAS1/GGP1/CWH52 results in a lower beta-glucan content of the cell wall and swollen, more spherical cells (L. Popolo, M. Vai, E. Gatti, S. Porello, P. Bonfante, R. Balestrini, and L. Alberghina, J. Bacteriol. 175:1879-1885, 1993; A. F. J. Ram, S. S. C. Brekelmans, L. J. W. M. Oehlen, and F. M. Klis, FEBS Lett. 358:165-170, 1995). We show here that gas1delta cells release beta1,3-glucan into the medium. Western analysis of the medium proteins with beta1,3-glucan- and beta1,6-glucan-specific antibodies showed further that at least some of the released beta1,3-glucan was linked to protein as part of a beta1,3-glucan-beta1,6-glucan-protein complex. These data indicate that Gas1p might play a role in the retention of beta1,3-glucan and/or beta-glucosylated proteins. Interestingly, the defective incorporation of beta1,3-glucan in the cell wall was accompanied by an increase in chitin and mannan content in the cell wall, an enhanced expression of cell wall protein 1 (Cwp1p), and an increase in beta1,3-glucan synthase activity, probably caused by the induced expression of Fks2p. It is proposed that the cell wall weakening caused by the loss of Gas1p induces a set of compensatory reactions to ensure cell integrity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Carbohydrate Metabolism
  • Carbohydrates / analysis
  • Cell Wall / chemistry
  • Cell Wall / metabolism
  • Chitin / metabolism
  • Cloning, Molecular
  • Culture Media, Conditioned / analysis
  • DNA, Fungal / genetics
  • DNA, Fungal / isolation & purification
  • Fungal Proteins / analysis
  • Fungal Proteins / metabolism
  • Gene Expression
  • Glucans / immunology
  • Glucans / metabolism*
  • Glucosyltransferases*
  • Glycoproteins / metabolism
  • Mannans / metabolism
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / metabolism*
  • Membrane Proteins / metabolism
  • Plasmids
  • Polymerase Chain Reaction
  • RNA, Fungal / analysis
  • Recombination, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Deletion
  • beta-Glucans*

Substances

  • CWP1 protein, S cerevisiae
  • Carbohydrates
  • Culture Media, Conditioned
  • DNA, Fungal
  • Fungal Proteins
  • GAS1 protein, S cerevisiae
  • Glucans
  • Glycoproteins
  • Mannans
  • Membrane Glycoproteins
  • Membrane Proteins
  • RNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • beta-Glucans
  • Chitin
  • beta-1,6-glucan
  • beta-1,3-glucan
  • Glucosyltransferases
  • GSC2 protein, S cerevisiae