Abstract
Meiosis and expression of early meiotic genes in the budding yeast Saccharomyces cerevisiae depend upon Rim11p, Ume6p, and Ime1p. Rim11p (also called Mds1p and ScGSK3) is a protein kinase related to glycogen synthase kinase 3 (GSK3); Ume6p is an architectural transcription factor; and Imelp is a Ume6p-binding protein that provides a transcriptional activation domain. Rim11p is required for Ime1p-Ume6p interaction, and prior studies have shown that Rim11p binds to and phosphorylates Ime1p. We show here that Rim11p binds to and phosphorylates Ume6p, as well. Amino acid substitutions in Ume6p that alter a consensus GSK3 site reduce or abolish Rim11p-Ume6p interaction and Rim11p-dependent phosphorylation, and they cause defects in interaction between Ume6p and Ime1p and in meiotic gene expression. Therefore, interaction between Rim11p and Ume6p, resulting in phosphorylation of Ume6p, is required for Ime1p-Ume6p complex formation. Rim11p, like metazoan GSK3beta, phosphorylates both interacting subunits of a target protein complex.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Base Sequence
-
Calcium-Calmodulin-Dependent Protein Kinases / genetics
-
Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / metabolism*
-
Fungal Proteins / genetics
-
Fungal Proteins / metabolism*
-
Gene Expression Regulation, Fungal
-
Glycogen Synthase Kinase 3
-
Glycogen Synthase Kinases
-
Intracellular Signaling Peptides and Proteins
-
Meiosis / genetics
-
Models, Biological
-
Oligodeoxyribonucleotides / genetics
-
Phosphorylation
-
Protein Binding
-
Protein Serine-Threonine Kinases / genetics
-
Protein Serine-Threonine Kinases / metabolism*
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Repressor Proteins*
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / metabolism*
-
Saccharomyces cerevisiae Proteins*
-
Transcription Factors / genetics
-
Transcription Factors / metabolism*
Substances
-
DNA-Binding Proteins
-
Fungal Proteins
-
Intracellular Signaling Peptides and Proteins
-
Oligodeoxyribonucleotides
-
Recombinant Fusion Proteins
-
Repressor Proteins
-
Saccharomyces cerevisiae Proteins
-
Transcription Factors
-
UME6 protein, S cerevisiae
-
Glycogen Synthase Kinases
-
Protein Serine-Threonine Kinases
-
RIM11 protein, S cerevisiae
-
Calcium-Calmodulin-Dependent Protein Kinases
-
Glycogen Synthase Kinase 3