Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59

Curr Genet. 1997 Nov;32(5):323-30. doi: 10.1007/s002940050284.

Abstract

A protein essential for pre-mRNA splicing, the U2 auxiliary factor (U2AF), is composed of a large and small subunit. Previously we cloned and characterized both subunits, spU2AF59 and spU2AF23, from fission yeast. We now report a novel U2AF-associated-protein, spUAP2, which interacts with both subunits. SpUAP2 contains a classical and a degenerate RNA recognition motif (RRM), both of which are required for interaction with spU2AF59. Interaction also requires the arginine/serine-rich region and the first RRM of spU2AF59. A null allele of the gene for spUAP2 is lethal.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites / genetics
  • DNA Primers / genetics
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes, Fungal*
  • Molecular Sequence Data
  • Nuclear Proteins*
  • RNA Splicing
  • RNA, Fungal / genetics
  • RNA, Fungal / metabolism
  • RNA-Binding Proteins*
  • Ribonucleoproteins / metabolism*
  • Saccharomyces cerevisiae / genetics
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Amino Acid
  • Splicing Factor U2AF

Substances

  • DNA Primers
  • DNA, Fungal
  • Fungal Proteins
  • Nuclear Proteins
  • RNA, Fungal
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Schizosaccharomyces pombe Proteins
  • Splicing Factor U2AF
  • UAP2 protein, S pombe

Associated data

  • GENBANK/U97681