Abstract
A protein essential for pre-mRNA splicing, the U2 auxiliary factor (U2AF), is composed of a large and small subunit. Previously we cloned and characterized both subunits, spU2AF59 and spU2AF23, from fission yeast. We now report a novel U2AF-associated-protein, spUAP2, which interacts with both subunits. SpUAP2 contains a classical and a degenerate RNA recognition motif (RRM), both of which are required for interaction with spU2AF59. Interaction also requires the arginine/serine-rich region and the first RRM of spU2AF59. A null allele of the gene for spUAP2 is lethal.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Binding Sites / genetics
-
DNA Primers / genetics
-
DNA, Fungal / genetics
-
Fungal Proteins / genetics*
-
Fungal Proteins / metabolism
-
Genes, Fungal*
-
Molecular Sequence Data
-
Nuclear Proteins*
-
RNA Splicing
-
RNA, Fungal / genetics
-
RNA, Fungal / metabolism
-
RNA-Binding Proteins*
-
Ribonucleoproteins / metabolism*
-
Saccharomyces cerevisiae / genetics
-
Schizosaccharomyces / genetics*
-
Schizosaccharomyces / metabolism*
-
Schizosaccharomyces pombe Proteins*
-
Sequence Homology, Amino Acid
-
Splicing Factor U2AF
Substances
-
DNA Primers
-
DNA, Fungal
-
Fungal Proteins
-
Nuclear Proteins
-
RNA, Fungal
-
RNA-Binding Proteins
-
Ribonucleoproteins
-
Schizosaccharomyces pombe Proteins
-
Splicing Factor U2AF
-
UAP2 protein, S pombe