Abstract
The structure of hus1+, a Schizosaccharomyces pombe gene required for S-M and DNA damage checkpoints, has been determined. Expression of hus1+ requires splicing of five exons, including a microexon that is only 13 nucleotides long. hus1+ is predicted to encode a 33 kDa protein with no similarity to sequences in any database, including the entire S. cerevisiae genome. Yeast strains disrupted for the hus1+ gene are viable but checkpoint-defective. Polyclonal antibodies were raised against bacterially expressed Hus1 protein, and used to study Hus1 regulation. Hus1 protein levels are not affected by S-phase arrest, and are not altered by mutations in other checkpoint genes, suggesting that Hus1 is not regulated at the transcriptional or translational levels.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Cell Cycle Proteins / chemistry
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Cell Cycle Proteins / genetics*
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Cell Cycle Proteins / metabolism
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Chromosome Mapping
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Cloning, Molecular
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DNA Damage / genetics*
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DNA, Complementary
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Exons / genetics
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Gene Expression Regulation, Fungal / genetics
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Genes, Fungal*
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Introns / genetics
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Microscopy, Fluorescence
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Mitosis*
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Molecular Sequence Data
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Mutation / genetics
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Open Reading Frames
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S Phase*
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Schizosaccharomyces / chemistry
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Schizosaccharomyces / genetics*
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Schizosaccharomyces pombe Proteins
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Transcription, Genetic / genetics
Substances
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Cell Cycle Proteins
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DNA, Complementary
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Schizosaccharomyces pombe Proteins
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hus1 protein, S pombe