Abstract
The commitment complex is the first defined step in the yeast (S. cerevisiae) splicing pathway. It contains U1 snRNP as well as Mud2p, which resembles human U2AF65. In a genetic screen, we identified the yeast gene MSL-5, which is a novel commitment complex component. Genetic and biochemical criteria indicate a direct interaction between Msl5p and both Mud2p and the U1 snRNP protein Prp40p. This defines a bridge between the two ends of the intron. Msl5p (renamed BBP for branchpoint bridging protein) has a mammalian ortholog, the splicing factor SF1. Our results show that SF1 interacts strongly with human U2AF65, and that SF1 is a bona fide E complex component. This implies that aspects of these novel cross-intron protein-protein interactions are conserved between yeast and mammals.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Conserved Sequence*
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DNA-Binding Proteins*
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Fungal Proteins / genetics
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Fungal Proteins / metabolism
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Humans
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Introns / genetics*
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Mammals
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Mutagenesis / physiology
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RNA Splicing / genetics
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RNA Splicing Factors
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Ribonucleoprotein, U1 Small Nuclear / genetics
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Ribonucleoprotein, U1 Small Nuclear / metabolism
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Ribonucleoproteins / genetics
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Ribonucleoproteins / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Splicing Factor U2AF
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Transcription Factors*
Substances
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DNA-Binding Proteins
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Fungal Proteins
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MUD2 protein, S cerevisiae
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RNA Splicing Factors
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RNA-Binding Proteins
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Recombinant Fusion Proteins
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Ribonucleoprotein, U1 Small Nuclear
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Ribonucleoproteins
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SF1 protein, S cerevisiae
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SF1 protein, human
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Saccharomyces cerevisiae Proteins
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Splicing Factor U2AF
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Transcription Factors