Abstract
We have determined the nucleotide sequence of a segment of VII of the yeast Saccharomyces cerevisiae contained in the cosmid clone pEGH101 for a total of 7 kbp. This sequence contains a large open reading frame (ORF) called G9365, coding for a protein of 1967 amino acids that shows a significant homology with the product of the SKI2 gene of S. cerevisiae and contains domains characteristics of RNA-helicases. The ORF is transcribed in vegetative cells but it is not essential for viability as demonstrated by gene disruption.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Chromosomes, Fungal / genetics*
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Fungal Proteins / genetics*
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Genes, Fungal / genetics*
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Molecular Sequence Data
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Open Reading Frames / genetics
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Phenotype
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RNA Helicases
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RNA Nucleotidyltransferases / genetics*
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RNA, Double-Stranded / analysis
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RNA, Fungal / analysis
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Spores, Fungal
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Transcription, Genetic
Substances
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Fungal Proteins
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RNA, Double-Stranded
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RNA, Fungal
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SKI2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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RNA Nucleotidyltransferases
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RNA Helicases
Associated data
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GENBANK/U35242
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SWISSPROT/P15938
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SWISSPROT/P24783
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SWISSPROT/P35207