RSC, an essential, abundant chromatin-remodeling complex

Cell. 1996 Dec 27;87(7):1249-60. doi: 10.1016/s0092-8674(00)81820-6.

Abstract

A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWII/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetyltransferases / metabolism
  • Adenosine Triphosphatases / chemistry*
  • Adenosine Triphosphate / metabolism
  • Amino Acid Sequence
  • Cell Cycle Proteins*
  • Chromatin / ultrastructure*
  • Chromosomal Proteins, Non-Histone / physiology*
  • DNA Helicases*
  • DNA-Binding Proteins / physiology*
  • Fungal Proteins / physiology*
  • Gene Expression Regulation, Fungal
  • Genes, Fungal
  • Histone Acetyltransferases
  • Macromolecular Substances
  • Mass Spectrometry
  • Molecular Sequence Data
  • Nuclear Proteins / physiology*
  • Nucleosomes / ultrastructure*
  • Protein Structure, Secondary
  • RNA Polymerase II / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Trans-Activators*
  • Transcription Factors / chemistry*
  • Transcription Factors / physiology*

Substances

  • Cell Cycle Proteins
  • Chromatin
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • Fungal Proteins
  • Macromolecular Substances
  • Nuclear Proteins
  • Nucleosomes
  • RSC complex, S cerevisiae
  • SNF12 protein, S cerevisiae
  • SWI3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • Adenosine Triphosphate
  • Acetyltransferases
  • Histone Acetyltransferases
  • RNA Polymerase II
  • Adenosine Triphosphatases
  • SNF2 protein, S cerevisiae
  • STH1 protein, S cerevisiae
  • DNA Helicases