Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae

Science. 1996 Nov 15;274(5290):1201-4. doi: 10.1126/science.274.5290.1201.

Abstract

Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans. A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36S particle that contains at least seven different proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase*
  • Cell Cycle Proteins / analysis
  • Cell Cycle Proteins / chemistry*
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Cell Division
  • Centrifugation, Density Gradient
  • Cyclins / metabolism
  • Fluorescent Antibody Technique, Indirect
  • Fungal Proteins / analysis
  • Fungal Proteins / chemistry*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • G1 Phase
  • Genes, Fungal
  • Ligases / chemistry*
  • Ligases / metabolism
  • Mitosis*
  • Saccharomyces cerevisiae / chemistry*
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / genetics
  • Ubiquitin-Protein Ligases
  • Ubiquitins / metabolism

Substances

  • BIME protein, Emericella nidulans
  • Cell Cycle Proteins
  • Cyclins
  • Fungal Proteins
  • Ubiquitins
  • Ubiquitin-Protein Ligases
  • Ligases