Abstract
Cell division in haploid yeast gives rise to a "mother" cell capable of mating-type switching and a "daughter" cell that is not. Switching is initiated by the HO endonuclease, whose gene is only transcribed in cells that have previously given birth to a bud (mother cells). HO expression depends on a minimyosin, She1p/Myo4p, which accumulates preferentially in growing buds. We describe a gene, ASH1, that is necessary to repress HO in daughters. ASH1 encodes a zinc finger protein whose preferential accumulation in daughter cell nuclei at the end of anaphase depends on She1p/Myo4p. The greater abundance of Ash1p in daughter cells is responsible for restricting HO expression to mother cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anaphase
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Base Sequence
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Cell Nucleus / metabolism*
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Cloning, Molecular
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Crosses, Genetic
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DNA-Binding Proteins*
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Deoxyribonucleases, Type II Site-Specific / biosynthesis*
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Deoxyribonucleases, Type II Site-Specific / genetics
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Epitopes / analysis
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Fungal Proteins / biosynthesis
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Fungal Proteins / metabolism*
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Gene Expression
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Genes, Fungal*
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Genes, Mating Type, Fungal*
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Molecular Sequence Data
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Mutagenesis
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Myosin Heavy Chains*
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Myosin Type V*
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Myosins / biosynthesis
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Myosins / metabolism*
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Open Reading Frames
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Repressor Proteins*
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Species Specificity
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Transcription Factors / biosynthesis*
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription, Genetic
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Zinc Fingers
Substances
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ASH1 protein, S cerevisiae
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DNA-Binding Proteins
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Epitopes
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Fungal Proteins
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Myo4 protein, S cerevisiae
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Recombinant Proteins
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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HO protein, S cerevisiae
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SCEI protein, S cerevisiae
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Deoxyribonucleases, Type II Site-Specific
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Myosin Type V
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Myosin Heavy Chains
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Myosins