Multiple transduction pathways regulate the sodium-extrusion gene PMR2/ENA1 during salt stress in yeast

FEBS Lett. 1996 Mar 11;382(1-2):89-92. doi: 10.1016/0014-5793(96)00157-3.

Abstract

The yeast PMR2/ENA1 gene encodes an ATPase involved in sodium extrusion and induced by NaCl. At low salt concentrations (0.3 M) induction is mediated by the HOG-MAP kinase pathway, a system activated by non-specific osmotic stress. At high salt concentrations (0.8 M) induction is mediated by the protein phosphatase calcineurin and is specific for sodium. Protein kinase A and Sis2p/Hal3p modulate PMR2/ENA1 expression as negative and positive factors, respectively but Sis2p/Hal3p does not participate in the transduction of the salt signal. Salt stress decreases the level of cAMP and the resulting decrease in protein kinase A activity may contribute to HOG-mediated induction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Calcineurin
  • Calcium-Calmodulin-Dependent Protein Kinases / physiology
  • Calmodulin-Binding Proteins / antagonists & inhibitors
  • Calmodulin-Binding Proteins / physiology
  • Cation Transport Proteins*
  • Cell Cycle Proteins*
  • Cyclic AMP-Dependent Protein Kinases / physiology
  • Enzyme Inhibitors / pharmacology
  • Fungal Proteins / physiology
  • Gene Expression Regulation, Fungal / drug effects*
  • Genes, Fungal / genetics
  • Genes, Reporter
  • Ion Transport
  • Osmotic Pressure
  • Phosphoprotein Phosphatases / antagonists & inhibitors
  • Phosphoprotein Phosphatases / physiology
  • Recombinant Fusion Proteins / biosynthesis
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction / physiology*
  • Sodium / metabolism
  • Sodium Chloride / pharmacology*
  • Sodium-Potassium-Exchanging ATPase
  • Tacrolimus / pharmacology
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Calmodulin-Binding Proteins
  • Cation Transport Proteins
  • Cell Cycle Proteins
  • ENA1 protein, S cerevisiae
  • Enzyme Inhibitors
  • Fungal Proteins
  • Recombinant Fusion Proteins
  • SIS2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sodium Chloride
  • Sodium
  • Cyclic AMP-Dependent Protein Kinases
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Calcineurin
  • Phosphoprotein Phosphatases
  • beta-Galactosidase
  • Adenosine Triphosphatases
  • Sodium-Potassium-Exchanging ATPase
  • Tacrolimus