Abstract
Iron must cross biological membranes to reach essential intracellular enzymes. Two proteins in the plasma membrane of yeast--a multicopper oxidase, encoded by the FET3 gene, and a permease, encoded by the FTR1 gene--were shown to mediate high-affinity iron uptake. FET3 expression was required for FTR1 protein to be transported to the plasma membrane. FTR1 expression was required for apo-FET3 protein to be loaded with copper and thus acquire oxidase activity. FTR1 protein also played a direct role in iron transport. Mutations in a conserved sequence motif of FTR1 specifically blocked iron transport.
MeSH terms
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Amino Acid Sequence
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Binding Sites
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Biological Transport
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism*
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Cell Membrane / metabolism
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Ceruloplasmin*
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Copper / metabolism
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Copper / pharmacology
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Endoplasmic Reticulum / metabolism
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Ferric Compounds / metabolism
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Ferritins / chemistry
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Ferritins / metabolism
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Ferrous Compounds / metabolism
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Genes, Fungal
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Golgi Apparatus / metabolism
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Iron / metabolism*
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Membrane Transport Proteins / chemistry
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Membrane Transport Proteins / genetics*
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Membrane Transport Proteins / metabolism*
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Models, Biological
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Molecular Sequence Data
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Multienzyme Complexes / metabolism*
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Mutation
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Open Reading Frames
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Oxidation-Reduction
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Oxidoreductases / metabolism*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Transformation, Genetic
Substances
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Carrier Proteins
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FTR1 protein, S cerevisiae
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Ferric Compounds
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Ferrous Compounds
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Membrane Transport Proteins
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Multienzyme Complexes
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Saccharomyces cerevisiae Proteins
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Copper
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Ferritins
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Iron
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Oxidoreductases
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Ceruloplasmin
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FET3 protein, S cerevisiae
Associated data
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GENBANK/P38993
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GENBANK/U18917
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GENBANK/X73124
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GENBANK/Z36076
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GENBANK/Z67998