Abstract
A sensitized genetic screen was carried out to identify essential genes involved in silencing in Saccharomyces cerevisiae. This screen identified temperature-sensitive alleles of ORC2 and ORC5, as described elsewhere, and ABF1, NPL3, and YCL54, as described here. Alleles of ABF1 that caused silencing defects provided the genetic proof of Abflp's role in silencing. The roles of Npl3p and Ycl54p are less clear. These proteins did not act exclusively through any one of the three protein binding sites of the HMR-E silencer. Unlike the orc2, orc5, and abf1 mutations that were isolated in the same (or a similar) screen for silencing mutants, neither temperature-sensitive mutation in NPL3 or YCL54 caused overt replication defects.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Amino Acid Sequence
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Cell Cycle
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Escherichia coli / genetics
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Fungal Proteins / genetics
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Fungal Proteins / physiology*
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Gene Expression Regulation, Fungal*
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Mating Factor
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Methyltransferases*
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Molecular Sequence Data
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Mutagenesis
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology*
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Peptides / genetics
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RNA-Binding Proteins*
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Regulatory Sequences, Nucleic Acid
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Temperature
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Transcription, Genetic*
Substances
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ABF1 protein, S cerevisiae
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DNA-Binding Proteins
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Fungal Proteins
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NPL3 protein, S cerevisiae
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Nuclear Proteins
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Peptides
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RNA-Binding Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Mating Factor
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Methyltransferases
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SPB1 protein, S cerevisiae