Abstract
Saccharomyces cerevisiae mutations that cause a requirement for SSD1-v for viability were isolated, yielding one new gene, LAS1, and three previously identified genes, SIT4, BCK1/SLK1, and SMP3. Three of these genes, LAS1, SIT4, and BCK1/SLK1, encode proteins that have roles in bud formation or morphogenesis. LAS1 is essential and loss of LAS1 function causes the cells to arrest as 80% unbudded cells and 20% large budded cells that accumulate many vesicles at the mother-daughter neck. Overexpression of LAS1 results in extra cell surface projections in the mother cell, alterations in actin and SPA2 localization, and the accumulation of electron-dense structures along the periphery of both the mother cell and the bud. The nuclear localization of LAS1 suggests a role of LAS1 for regulating bud formation and morphogenesis via the expression of components that function directly in these processes.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Cell Size
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Cell Wall / metabolism
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Fungal Proteins / genetics
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Fungal Proteins / physiology*
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Genes, Fungal*
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Mitogen-Activated Protein Kinase Kinases*
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Molecular Sequence Data
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Morphogenesis
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology*
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Phosphoprotein Phosphatases / genetics
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Phosphoprotein Phosphatases / physiology
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Protein Kinases / genetics
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Protein Kinases / physiology
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Protein Phosphatase 2
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Recombinant Fusion Proteins / biosynthesis
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Saccharomyces cerevisiae / cytology*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae Proteins*
Substances
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Fungal Proteins
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LAS1 protein, S cerevisiae
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Nuclear Proteins
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Recombinant Fusion Proteins
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SMP3 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Protein Kinases
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BCK1 protein, S cerevisiae
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Mitogen-Activated Protein Kinase Kinases
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Phosphoprotein Phosphatases
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Protein Phosphatase 2
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SIT4 protein, S cerevisiae