Abstract
Alpha factor induces arrest of yeast a cells in G1 and transcription of genes involved in mating. Prior work indicates that FUS3, a member of the MAP kinase family, and FAR1, whose molecular activity is unknown, contribute to cell cycle arrest by inhibiting G1 cyclins. Here we show that FAR1 is a substrate for FUS3 and that this phosphorylation regulates association of FAR1 with CDC28-CLN2 kinase. We show also that FAR1 is phosphorylated in vitro by the CDC28-CLN2 complex and in vivo in a CDC28-dependent manner. Mutational analysis of FAR1 reveals a correlation between its ability to associate with CDC28-CLN2 and to arrest the cell cycle. These results suggest that FAR1 protein is the link between the signaling pathway and the cell cycle machinery.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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CDC28 Protein Kinase, S cerevisiae / metabolism
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Cell Cycle / physiology*
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Cell Cycle Proteins*
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Cyclin-Dependent Kinase Inhibitor Proteins
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DNA Mutational Analysis
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Fungal Proteins / metabolism*
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Mating Factor
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Mitogen-Activated Protein Kinases*
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Molecular Sequence Data
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Peptides
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Phosphorylation
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Precipitin Tests
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Protein Binding
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Repressor Proteins*
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins*
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Signal Transduction / physiology*
Substances
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Cell Cycle Proteins
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Cyclin-Dependent Kinase Inhibitor Proteins
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FAR1 protein, S cerevisiae
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Fungal Proteins
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Peptides
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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Mating Factor
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CDC28 Protein Kinase, S cerevisiae
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FUS3 protein, S cerevisiae
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Mitogen-Activated Protein Kinases