The yeast SNF2/SWI2 protein has DNA-stimulated ATPase activity required for transcriptional activation

Genes Dev. 1993 Apr;7(4):583-91. doi: 10.1101/gad.7.4.583.

Abstract

The yeast SNF2 (SWI2) protein functions with SNF5, SNF6, SWI1, and SWI3 in the transcriptional activation of many differently regulated genes. These proteins appear to facilitate activation by gene-specific regulatory proteins. SNF2 is highly conserved among eukaryotes and defines a family of proteins with similarity to helicases and nucleic acid-dependent NTPases. Here, we present genetic and biochemical evidence that SNF2 has DNA-stimulated ATPase activity. Mutations in the nucleoside triphosphate (NTP)-binding motif and other conserved motifs impair SNF2 function. Swapping experiments with another member of this family indicate that the helicase-related domains are functionally interchangeable. Finally, bacterially expressed SNF2 protein has ATPase activity that is stimulated by double-stranded DNA, and mutation of the NTP-binding site abolishes this activity. Deletion analysis shows that the helicase-like region of SNF2 is necessary, but not sufficient, for transcriptional activation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Adenosine Triphosphate / metabolism*
  • Base Sequence
  • Binding Sites / genetics
  • Cell Cycle Proteins*
  • Chromatin / metabolism
  • Conserved Sequence
  • DNA Helicases / genetics
  • DNA Mutational Analysis
  • DNA, Fungal / analysis
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / physiology
  • Fungal Proteins / genetics*
  • Hydrolysis
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nuclear Proteins*
  • Oligonucleotide Probes
  • Protein Binding
  • Recombinant Fusion Proteins / physiology
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / genetics*
  • Transcription Factors / physiology
  • Transcription, Genetic*

Substances

  • Cell Cycle Proteins
  • Chromatin
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Nuclear Proteins
  • Oligonucleotide Probes
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Adenosine Triphosphate
  • Adenosine Triphosphatases
  • SNF2 protein, S cerevisiae
  • STH1 protein, S cerevisiae
  • DNA Helicases