Abstract
Cyclophilins are cyclosporin A sensitive peptidyl-prolyl cis-trans isomerases found in the cytoplasm of a wide range of species from Escherichia coli to man. Cyclophilin homologues are translocated into mitochondria, the endoplasmic reticulum and the bacterial periplasmic space. Here, the nucleotide sequence of a non-essential fifth Saccharomyces cerevisiae cyclophilin homologue encoded by chromosome XII is presented. As expected from its hydrophobic signal sequence and a hydrophilic carboxy terminus ending in the tetrapeptide HDEL, epitope-tagged cyclophilin D was found associated with the endoplasmic reticulum.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Isomerases / genetics*
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Amino Acid Isomerases / isolation & purification
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Amino Acid Isomerases / metabolism
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Amino Acid Sequence
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Base Sequence
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Biological Transport
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Carrier Proteins / genetics*
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism
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Cell Compartmentation
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Endoplasmic Reticulum / enzymology
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Endoplasmic Reticulum / metabolism
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Genes, Fungal / genetics*
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Immunohistochemistry
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Molecular Sequence Data
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Multigene Family / genetics*
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Oligopeptides / genetics
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Oligopeptides / immunology
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Peptidylprolyl Isomerase
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Sequence Homology, Amino Acid
Substances
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Carrier Proteins
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Oligopeptides
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Amino Acid Isomerases
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Peptidylprolyl Isomerase