Abstract
Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously known protein have been noted. The protein is very hydrophobic and is predicted to have a membraneous localisation. In agreement with this hypothesis, the analysis of membrane proteins from stationary-phase cells showed that a strain carrying this gene on a multicopy vector overproduces a protein of 30 kDa. This protein was recognized by antibodies directed against the N-terminal portion of the gene product. Considering its induction in response to heat shock and the apparent molecular weight of its product, this gene was designated HSP30.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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Codon
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DNA, Fungal / isolation & purification
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Gene Expression Regulation, Fungal
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Genes, Fungal*
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HSP30 Heat-Shock Proteins
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Heat-Shock Proteins / genetics*
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Heat-Shock Proteins / metabolism
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins
Substances
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Codon
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DNA, Fungal
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Fungal Proteins
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HSP30 Heat-Shock Proteins
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HSP30 protein, S cerevisiae
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Heat-Shock Proteins
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Membrane Proteins
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Saccharomyces cerevisiae Proteins
Associated data
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GENBANK/M93123
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GENBANK/X68488
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GENBANK/X68489
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GENBANK/X68490
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GENBANK/X71675
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GENBANK/X71676
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GENBANK/X71677
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GENBANK/X71678
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GENBANK/X71679
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GENBANK/X71680