Abstract
Transmission of an external signal from receptors to downstream targets is often mediated by a conserved set of protein kinases that act in sequence (a kinase cascade). In haploid strains of Saccharomyces cerevisiae, a signal initiated by peptide pheromones is transmitted through this kinase cascade to a transcription factor STE12, which is required for the expression of many mating-specific genes. Here it was shown that in diploids some of the same kinases and STE12 are required for filamentous growth, but the pheromone receptors and guanosine triphosphate-binding protein are not required for filament formation. Thus, a similar kinase cascade is activated by different signals in haploids and diploids and mediates different developmental outcomes in the two cell types.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Fungal Proteins / metabolism*
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GTP-Binding Proteins / metabolism
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Genes, Fungal
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Mating Factor
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Mutation
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Peptides / metabolism*
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Peptides / pharmacology
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Phenotype
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Protein Kinases / metabolism*
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Receptors, Mating Factor
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Receptors, Peptide / metabolism
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Signal Transduction*
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Transcription Factors / metabolism*
Substances
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Fungal Proteins
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Peptides
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Receptors, Mating Factor
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Receptors, Peptide
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STE12 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Mating Factor
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Protein Kinases
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GTP-Binding Proteins