Identification and characterization of a novel yeast gene: the YGP1 gene product is a highly glycosylated secreted protein that is synthesized in response to nutrient limitation

Mol Cell Biol. 1994 Apr;14(4):2740-54. doi: 10.1128/mcb.14.4.2740-2754.1994.

Abstract

Nutrient starvation in the yeast Saccharomyces cerevisiae leads to a number of physiological changes that accompany entry into stationary phase. The expression of genes whose products play a role in stress adaptation is regulated in a manner that allows the cell to sense and respond to changing environmental conditions. We have identified a novel yeast gene, YGP1, that displays homology to the sporulation-specific SPS100 gene. The expression of YGP1 is regulated by nutrient availability. The gene is expressed at a basal level during "respiro-fermentative" (logarithmic) growth. When the glucose concentration in the medium falls below 1%, the YGP1 gene is derepressed and the gene product, gp37, is synthesized at levels up to 50-fold above the basal level. The glucose-sensing mechanism is independent of the SNF1 pathway and does not operate when cells are directly shifted to a low glucose concentration. The expression of YGP1 also responds to the depletion of nitrogen and phosphate, indicating a general response to nutrient deprivation. These results suggest that the YGP1 gene product may be involved in cellular adaptations prior to stationary phase and may be a useful marker protein for monitoring early events associated with the stress response.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alkaline Phosphatase / biosynthesis
  • Alkaline Phosphatase / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Western
  • Cloning, Molecular
  • Culture Media
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • Escherichia coli
  • Fermentation
  • Fungal Proteins / biosynthesis*
  • Fungal Proteins / genetics*
  • Fungal Proteins / isolation & purification
  • Gene Expression / drug effects
  • Genes, Fungal*
  • Glucose / metabolism
  • Glucose / pharmacology
  • Glycoproteins / biosynthesis*
  • Glycoproteins / genetics*
  • Glycoproteins / isolation & purification
  • Glycoside Hydrolases / biosynthesis
  • Glycoside Hydrolases / genetics
  • Glycosylation
  • Kinetics
  • Molecular Sequence Data
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / metabolism
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Spores, Fungal / physiology
  • Trehalase / genetics
  • beta-Fructofuranosidase

Substances

  • Culture Media
  • DNA, Fungal
  • Fungal Proteins
  • Glycoproteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • YGP1 protein, S cerevisiae
  • Alkaline Phosphatase
  • Glycoside Hydrolases
  • beta-Fructofuranosidase
  • Trehalase
  • Glucose

Associated data

  • GENBANK/X73030