Abstract
Nutrient starvation in the yeast Saccharomyces cerevisiae leads to a number of physiological changes that accompany entry into stationary phase. The expression of genes whose products play a role in stress adaptation is regulated in a manner that allows the cell to sense and respond to changing environmental conditions. We have identified a novel yeast gene, YGP1, that displays homology to the sporulation-specific SPS100 gene. The expression of YGP1 is regulated by nutrient availability. The gene is expressed at a basal level during "respiro-fermentative" (logarithmic) growth. When the glucose concentration in the medium falls below 1%, the YGP1 gene is derepressed and the gene product, gp37, is synthesized at levels up to 50-fold above the basal level. The glucose-sensing mechanism is independent of the SNF1 pathway and does not operate when cells are directly shifted to a low glucose concentration. The expression of YGP1 also responds to the depletion of nitrogen and phosphate, indicating a general response to nutrient deprivation. These results suggest that the YGP1 gene product may be involved in cellular adaptations prior to stationary phase and may be a useful marker protein for monitoring early events associated with the stress response.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alkaline Phosphatase / biosynthesis
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Alkaline Phosphatase / genetics
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Cloning, Molecular
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Culture Media
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DNA, Fungal / genetics
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DNA, Fungal / metabolism
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Escherichia coli
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Fermentation
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Fungal Proteins / biosynthesis*
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Fungal Proteins / genetics*
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Fungal Proteins / isolation & purification
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Gene Expression / drug effects
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Genes, Fungal*
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Glucose / metabolism
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Glucose / pharmacology
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Glycoproteins / biosynthesis*
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Glycoproteins / genetics*
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Glycoproteins / isolation & purification
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Glycoside Hydrolases / biosynthesis
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Glycoside Hydrolases / genetics
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Glycosylation
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Kinetics
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Molecular Sequence Data
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / metabolism
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Restriction Mapping
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Homology, Amino Acid
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Spores, Fungal / physiology
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Trehalase / genetics
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beta-Fructofuranosidase
Substances
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Culture Media
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DNA, Fungal
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Fungal Proteins
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Glycoproteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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YGP1 protein, S cerevisiae
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Alkaline Phosphatase
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Glycoside Hydrolases
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beta-Fructofuranosidase
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Trehalase
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Glucose