Abstract
Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA library of Schizosaccharomyces pombe by subtractive screening. Transcription of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dependent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA primary structures revealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino-acid sequences of isp5 and isp6 were found to show significant similarities to those of amino-acid permeases and proteinase B of Saccharomyces cerevisiae, respectively. Disruption of isp6 arrested the cell cycle prior to conjugation and caused a drastic blocking effect on spore formation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acid Transport Systems
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Carrier Proteins*
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Cell Cycle
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DNA, Complementary / genetics
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DNA, Fungal / genetics
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Fungal Proteins / genetics*
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Gene Expression Regulation, Fungal*
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Genes, Fungal*
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Membrane Transport Proteins / genetics*
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Molecular Sequence Data
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / physiology
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Schizosaccharomyces pombe Proteins*
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Sequence Alignment
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Sequence Homology, Amino Acid
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Serine Endopeptidases / genetics*
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Sex Differentiation / genetics
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Spores, Fungal
Substances
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Amino Acid Transport Systems
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Carrier Proteins
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DNA, Complementary
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DNA, Fungal
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Fungal Proteins
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ISP3 protein, S pombe
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Membrane Transport Proteins
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Schizosaccharomyces pombe Proteins
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isp4 protein, S pombe
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isp7 protein, S pombe
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Serine Endopeptidases
Associated data
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GENBANK/D14060
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GENBANK/D14061
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GENBANK/D14062
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GENBANK/D14063
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GENBANK/D14064