The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition

Genes Dev. 1994 Apr 1;8(7):843-54. doi: 10.1101/gad.8.7.843.

Abstract

In characterizing a series of yeast (Saccharomyces cerevisiae) mutants synthetic lethal with U1 RNA, we have identified a yeast gene (MUD2) with sequence similarity to the well-studied metazoan splicing factor U2AF65. The biochemical characterization indicates that the MUD2 gene product (MUD2P) contacts pre-mRNA directly and is a component of the pre-mRNA-U1 snRNP complex (commitment complex) that forms during early spliceosome assembly in yeast extracts. Unlike U1 snRNP itself, the association of MUD2P with pre-mRNA is dependent on a proper yeast branchpoint sequence. Genetic experiments indicate that MUD2P affects U2 snRNP addition. Moreover, experiments in the two-hybrid system show that PRP11P, a recently identified component of U2 snRNP, can interact directly with MUD2P. The experiments identify a specific inter-snRNP protein-protein contact that occurs during spliceosome assembly and more generally support substantial functional similarity between U2AF65 and MUD2P.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cloning, Molecular
  • Consensus Sequence
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Genes, Lethal
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral / metabolism
  • Models, Genetic
  • Molecular Sequence Data
  • RNA Precursors / metabolism*
  • RNA Processing, Post-Transcriptional
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins / biosynthesis
  • Ribonucleoprotein, U1 Small Nuclear / metabolism
  • Ribonucleoprotein, U2 Small Nuclear / metabolism*
  • Ribonucleoproteins / chemistry
  • Ribonucleoproteins / genetics
  • Ribonucleoproteins / metabolism*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Spliceosomes / metabolism
  • Splicing Factor U2AF
  • Viral Envelope Proteins / metabolism

Substances

  • Fungal Proteins
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Hemagglutinins, Viral
  • MUD2 protein, S cerevisiae
  • PRP11 protein, S cerevisiae
  • RNA Precursors
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Ribonucleoprotein, U1 Small Nuclear
  • Ribonucleoprotein, U2 Small Nuclear
  • Ribonucleoproteins
  • Saccharomyces cerevisiae Proteins
  • Splicing Factor U2AF
  • Viral Envelope Proteins

Associated data

  • GENBANK/U22449