The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence

Mol Cell Biol. 1983 Mar;3(3):439-47. doi: 10.1128/mcb.3.3.439-447.1983.

Abstract

The SUC2 gene of Saccharomyces cerevisiae encodes two differently regulated mRNAs (1.8 and 1.9 kilobases) that differ at their 5' ends. The larger RNA encodes a secreted, glycosylated form of invertase and the smaller RNA encodes an intracellular, nonglycosylated form. We have determined the nucleotide sequence of the amino-terminal coding region of the SUC2 gene and its upstream flanking region and have mapped the 5' ends of the SUC2 mRNAs relative to the DNA sequence. The 1.9-kilobase RNA contains a signal peptide coding sequence and presumably encodes a precursor to secreted invertase. The 1.8-kilobase RNA does not include the complete coding sequence for the signal peptide. The nucleotide sequence data prove that SUC2 is a structural gene for invertase, and translation of the coding information provides the complete amino acid sequence of an S. cerevisiae signal peptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Genes
  • Glycoside Hydrolases / biosynthesis*
  • Glycoside Hydrolases / genetics
  • RNA, Messenger / metabolism*
  • Saccharomyces cerevisiae / enzymology*
  • beta-Fructofuranosidase

Substances

  • RNA, Messenger
  • Glycoside Hydrolases
  • beta-Fructofuranosidase

Associated data

  • GENBANK/K00540
  • GENBANK/V01311