Abstract
In these experiments we demonstrate that purified RAS proteins, whether derived from the yeast RAS1 or RAS2 or the human H-ras genes, activate yeast adenylate cyclase in the presence of guanine nucleotides. These results confirm the prediction of earlier genetic and biochemical data and for the first time provide a complete biochemical assay for RAS protein function. Furthermore, we observe a biochemical difference between the RAS2 and RAS2val19 proteins in their ability to activate adenylate cyclase after preincubation with GTP.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenylyl Cyclases / metabolism*
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Enzyme Activation / drug effects
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Fungal Proteins / genetics
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Fungal Proteins / isolation & purification
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Fungal Proteins / pharmacology*
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Guanine Nucleotides / pharmacology
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Humans
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Membrane Proteins / genetics
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Membrane Proteins / isolation & purification
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Membrane Proteins / pharmacology*
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Mutation
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Oncogenes*
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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ras Proteins*
Substances
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Fungal Proteins
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Guanine Nucleotides
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Membrane Proteins
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Saccharomyces cerevisiae Proteins
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RAS1 protein, S cerevisiae
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RAS2 protein, S cerevisiae
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ras Proteins
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Adenylyl Cyclases