GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae

J Bacteriol. 1986 Apr;166(1):313-8. doi: 10.1128/jb.166.1.313-318.1986.

Abstract

The gene encoding the galactose permease of Saccharomyces cerevisiae (GAL2) was cloned. The clone restores galactose permease activity to gal2 yeasts and is regulated by galactose in a manner similar to other GAL gene products (GAL1, -7, and -10). Experiments with temperature-conditional secretory mutants indicated that transport of the GAL2 gene product to the cell surface requires a functional secretory pathway. In addition, gene fusions were constructed between the GAL2 gene and the Escherichia coli lacZ gene. The GAL2-lacZ gene fusions code for galactose-regulated beta-galactosidase activity in yeasts. The beta-galactosidase activity was found to be membrane bound.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biological Transport
  • Cell Membrane / enzymology
  • Genes
  • Genes, Fungal*
  • Genetic Engineering
  • Membrane Transport Proteins / biosynthesis
  • Membrane Transport Proteins / genetics*
  • Monosaccharide Transport Proteins
  • Mutation
  • Plasmids
  • Promoter Regions, Genetic
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • beta-Galactosidase / analysis
  • beta-Galactosidase / genetics

Substances

  • Membrane Transport Proteins
  • Monosaccharide Transport Proteins
  • galactose permease
  • beta-Galactosidase