A yeast actin-binding protein is encoded by SAC6, a gene found by suppression of an actin mutation

Science. 1989 Jan 13;243(4888):231-3. doi: 10.1126/science.2643162.

Abstract

The protein encoded by SAC6, a gene that can mutate to suppress a temperature-sensitive defect in the yeast actin gene, has been identified as a 67-kilodalton actin-binding protein (ABP 67) that associates with all identifiable actin structures. This finding demonstrates the in vivo functional importance of the actin-ABP 67 interaction previously established in vitro and illustrates the use of suppressor analysis to identify physically interacting proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / genetics*
  • Actins / metabolism
  • Genes*
  • Genes, Fungal*
  • Microfilament Proteins / genetics*
  • Microfilament Proteins / metabolism
  • Mutation*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Suppression, Genetic*

Substances

  • Actins
  • Microfilament Proteins