Abstract
The gene encoding ISP42, an integral outermembrane protein located at the yeast mitochondrial protein import site was cloned, sequenced and modified. Yeast cells depleted of ISP42 accumulate uncleaved mitochondrial precursor proteins and then die. ISP42 is the first mitochondrial membrane protein shown to be indispensable for protein import and cell viability.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
-
Fungal Proteins / genetics*
-
Fungal Proteins / metabolism
-
Genes, Fungal*
-
Kinetics
-
Membrane Proteins / genetics
-
Membrane Transport Proteins*
-
Mitochondria / metabolism*
-
Mitochondrial Membrane Transport Proteins
-
Molecular Sequence Data
-
Restriction Mapping
-
Saccharomyces cerevisiae / drug effects
-
Saccharomyces cerevisiae / genetics*
-
Saccharomyces cerevisiae / growth & development
-
Saccharomyces cerevisiae Proteins*
Substances
-
Fungal Proteins
-
Membrane Proteins
-
Membrane Transport Proteins
-
Mitochondrial Membrane Transport Proteins
-
Saccharomyces cerevisiae Proteins
-
Tom40 protein, S cerevisiae
-
Carbonyl Cyanide m-Chlorophenyl Hydrazone