srd1, a Saccharomyces cerevisiae suppressor of the temperature-sensitive pre-rRNA processing defect of rrp1-1

Genetics. 1990 Mar;124(3):497-504. doi: 10.1093/genetics/124.3.497.

Abstract

We define a new gene, SRD1, involved in the processing of pre-rRNA to mature rRNA. The SRD1 gene was identified by selecting for second-site suppressors of the previously described rrp1-1 mutation. The rrp1-1 mutation causes temperature-sensitive growth, a conditional defect in processing of 27S pre-rRNA to mature 25S rRNA, and a nonconditional increase in sensitivity to several aminoglycoside antibiotics. All srd1 alleles identified are recessive and apparently specific to the rrp1-1 mutation. Although a mutation of SRD1 suppresses the pre-rRNA processing defect, drug sensitivity and thermolethality of a point mutation of RRP1, it is unable to suppress a rrp1-disruption allele. We suggest that the SRD1 gene product either interacts with or regulates the RRP1 product.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Base Sequence
  • Blotting, Southern
  • Crosses, Genetic
  • Genes, Fungal
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • RNA Precursors / metabolism*
  • RNA Processing, Post-Transcriptional*
  • RNA, Ribosomal / metabolism*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / growth & development
  • Suppression, Genetic*
  • Temperature

Substances

  • RNA Precursors
  • RNA, Ribosomal