Abstract
Subcellular localization of enzymes of arginine metabolism in Saccharomyces cerevisiae was studied by partial fractionation and stepwise homogenization of spheroplast lysates. These enzymes could clearly be divided into two groups. The first group comprised the five enzymes of the acetylated compound cycle, i.e., acetylglutamate synthase, acetylglutamate kinase, acetylglutamyl-phosphate reductase, acetylornithine aminotransferase, and acetylornithine-glutamate acetyltransferase. These enzymes were exclusively particulate. Comparison with citrate synthase and cytochrome oxidase, and results from isopycnic gradient analysis, suggested that these enzymes were associated with the mitochondria. By contrast, enzymatic activities going from ornithine to arginine, i.e., arginine pathway-specific carbamoylphosphate synthetase, ornithine carbamoyltransferase, argininosuccinate synthetase, and argininosuccinate lyase, and the two first catabolic enzymes, arginase and ornithine aminotransferase, were in the "soluble" fraction of the cell.
MeSH terms
-
Acetamides
-
Acetyl Coenzyme A
-
Acetyltransferases / analysis
-
Aldehyde Oxidoreductases / analysis
-
Arginase / analysis
-
Argininosuccinate Lyase / analysis
-
Argininosuccinate Synthase / analysis
-
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / analysis
-
Glutamates
-
Mitochondria / enzymology
-
Organophosphorus Compounds
-
Ornithine
-
Ornithine Carbamoyltransferase / analysis
-
Ornithine-Oxo-Acid Transaminase / analysis
-
Phosphotransferases (Carboxyl Group Acceptor)
-
Phosphotransferases / analysis
-
Saccharomyces cerevisiae / enzymology*
-
Subcellular Fractions / enzymology
-
Transaminases / analysis
Substances
-
Acetamides
-
Glutamates
-
Organophosphorus Compounds
-
Acetyl Coenzyme A
-
Ornithine
-
Aldehyde Oxidoreductases
-
N-acetyl-gamma-glutamyl-phosphate reductase
-
Ornithine Carbamoyltransferase
-
Acetyltransferases
-
glutamate acetyltransferase
-
Transaminases
-
Ornithine-Oxo-Acid Transaminase
-
Phosphotransferases
-
Phosphotransferases (Carboxyl Group Acceptor)
-
acetylglutamate kinase
-
Arginase
-
Argininosuccinate Lyase
-
Argininosuccinate Synthase
-
Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)