Abstract
We have investigated the transport of maltose in a genetically defined maltose-fermenting strain of Saccharomyces cerevisiae carrying the MAL1 locus. Two kinetically different systems were identified: a high-affinity transporter with a Km of 4 mM and a low-affinity transporter with a Km of 70 to 80 mM. The high-affinity maltose transporter is maltose inducible and is encoded by the MAL11 (and/or MAL61) gene of the MAL1 (and/or MAL6) locus. The low-affinity maltose transporter is expressed constitutively and is not related to MAL11 and/or MAL61. Both maltose transporters are subject to glucose-induced inactivation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Biological Transport / drug effects
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Carrier Proteins / genetics*
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Fungal Proteins / genetics*
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Genes, Fungal
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Glucose / pharmacology
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Kinetics
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Maltose / metabolism
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Monosaccharide Transport Proteins*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Symporters*
Substances
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Carrier Proteins
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Fungal Proteins
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Monosaccharide Transport Proteins
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Saccharomyces cerevisiae Proteins
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Symporters
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maltose transport system, S cerevisiae
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Maltose
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Glucose