Abstract
Schizosaccharomyces pombe has four alpha-amylase homologs (Aah1p-Aah4p) with a glycosylphosphatidylinositol (GPI) modification site at the C-terminal end. Disruption mutants of aah genes were tested for mislocalization of vacuolar carboxypeptidase Y (CPY), and aah3Delta was found to secrete CPY. The conversion rate from pro- to mature CPY was greatly impaired in aah3Delta, and fluorescence microscopy inidicated that a sorting receptor for CPY, Vps10p, mislocalized to the vacuolar membrane. These results indicate that aah3Delta had a defect in the retrograde transport of Vps10p, and that Aah3p is the first S. pombe specific protein required for vacuolar protein sorting.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cathepsin A / metabolism
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Fluorescence
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Glycosylphosphatidylinositols / genetics
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Glycosylphosphatidylinositols / physiology*
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Membrane Proteins / genetics
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Membrane Proteins / physiology*
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Microscopy, Fluorescence
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Mutation / physiology
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Plasmids / genetics
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Schizosaccharomyces / genetics
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Schizosaccharomyces / metabolism*
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Schizosaccharomyces pombe Proteins / genetics
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Schizosaccharomyces pombe Proteins / metabolism
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Schizosaccharomyces pombe Proteins / physiology*
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Vacuoles / genetics
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Vacuoles / metabolism*
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alpha-Amylases / genetics
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alpha-Amylases / metabolism
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alpha-Amylases / physiology*
Substances
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Glycosylphosphatidylinositols
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Membrane Proteins
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Schizosaccharomyces pombe Proteins
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AAH3 protein, S pombe
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alpha-Amylases
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Cathepsin A