Abstract
In eukaryotes, termination of messenger RNA (mRNA) translation is mediated by the release factors eRF1 and eRF3. Using Saccharomyces cerevisiae as a model organism, we have identified a member of the DEAD-box protein (DBP) family, the DEAD-box RNA helicase and mRNA export factor Dbp5, as a player in translation termination. Dbp5 interacts genetically with both release factors and the polyadenlyate-binding protein Pab1. A physical interaction was specifically detected with eRF1. Moreover, we show that the helicase activity of Dbp5 is required for efficient stop-codon recognition, and intact Dbp5 is essential for recruitment of eRF3 into termination complexes. Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Codon, Terminator
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DEAD-box RNA Helicases / genetics
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DEAD-box RNA Helicases / metabolism*
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Mutation
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Nucleocytoplasmic Transport Proteins / genetics
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Nucleocytoplasmic Transport Proteins / metabolism*
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Peptide Chain Termination, Translational*
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Peptide Termination Factors / genetics
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Peptide Termination Factors / metabolism
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Poly(A)-Binding Proteins / genetics
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Poly(A)-Binding Proteins / metabolism
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Polyribosomes / metabolism
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RNA Helicases / genetics
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RNA Helicases / metabolism*
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RNA Stability
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RNA, Fungal / metabolism
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RNA, Messenger / metabolism
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
Substances
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Codon, Terminator
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Nucleocytoplasmic Transport Proteins
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Peptide Termination Factors
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Poly(A)-Binding Proteins
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RNA, Fungal
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RNA, Messenger
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SUP45 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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pab1 protein, S cerevisiae
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peptide-chain-release factor 3
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DBP5 protein, S cerevisiae
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NAM7 protein, S cerevisiae
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DEAD-box RNA Helicases
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RNA Helicases