A role for fission yeast Rab GTPase Ypt7p in sporulation

Jun Kashiwazaki; Taro Nakamura; Tomoko Iwaki; Kaoru Takegawa; Chikashi Shimoda

doi:10.1247/csf.30.43

A role for fission yeast Rab GTPase Ypt7p in sporulation

Cell Struct Funct. 2005;30(2):43-9. doi: 10.1247/csf.30.43.

Authors

Jun Kashiwazaki¹, Taro Nakamura, Tomoko Iwaki, Kaoru Takegawa, Chikashi Shimoda

Affiliation

¹ Department of Biology, Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.

PMID: 16357443
DOI: 10.1247/csf.30.43

Abstract

Ypt7p, a fission yeast (Schizosaccharomyces pombe) homologue of Rab7 GTPase, mediates fusion of endosomes to vacuoles and homotypic vacuole fusion. Here, we report that Ypt7p plays important roles in sporulation. Most ypt7Delta asci produced less than four spores, which were apparently immature and germinated at low frequency. Furthermore, ypt7Delta cells were defective in development of the forespore membranes. Vacuoles in sporulating cells were found to undergo extensive homotypic vacuole fusion to form a few large compartments occupying the entire cytoplasm of asci. This extensive vacuole fusion depended on Ypt7p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Fungal
Membrane Fusion / genetics
Membrane Fusion / physiology*
Microscopy, Fluorescence
Mutation
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism
Saccharomyces cerevisiae Proteins / physiology*
Schizosaccharomyces / cytology
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism*
Spores, Fungal / physiology*
Vacuoles / physiology
rab GTP-Binding Proteins / genetics
rab GTP-Binding Proteins / metabolism
rab GTP-Binding Proteins / physiology*

Substances

Saccharomyces cerevisiae Proteins
YPT7 protein, S cerevisiae
rab GTP-Binding Proteins